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11 pages, 1200 KB  
Article
Estimating Whale Shark, Rhincodon typus, Length Using Multi-Stereo-Image Measurement
by Hiroto Yamamoto, Akira Sasaki, Tomoki Kanna, Yasushi Mitsunaga and Shinsuke Torisawa
Fishes 2025, 10(10), 513; https://doi.org/10.3390/fishes10100513 - 10 Oct 2025
Abstract
The whale shark Rhincodon typus is the largest known extant omnivorous fish species, reaching up to 17 m in length. Because of its slow growth and late maturity, R. typus is particularly vulnerable to human activities and is listed as endangered on the [...] Read more.
The whale shark Rhincodon typus is the largest known extant omnivorous fish species, reaching up to 17 m in length. Because of its slow growth and late maturity, R. typus is particularly vulnerable to human activities and is listed as endangered on the IUCN Red List. Understanding its biological characteristics, such as growth rate, is essential for their conservation. Non-invasive methods, including stereo-image measurements, have been used to measure the body length of the species over the years, which aggregates in coastal areas during specific life stages. This method enables us to estimate fish length by recording the target using a stereo camera, which commonly consists of two cameras. However, measurement errors increase in the setup as the target moves away from the camera. Therefore, we conducted a multi-stereo video shoot of a free-swimming whale shark in an aquarium tank and compared the performance of stereo cameras using two, three, and four cameras. The setups with three and four cameras outperformed the traditional two-camera stereo setup in terms of precision and accuracy, suggesting that a multi-stereo camera system can effectively estimate the body length of large animals such as whale sharks from a considerable distance. Full article
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25 pages, 1350 KB  
Article
Economic and Biological Impact of Eradication Measures for Xylella fastidiosa in Northern Portugal
by Talita Loureiro, Luís Serra, José Eduardo Pereira, Ângela Martins, Isabel Cortez and Patrícia Poeta
Environments 2025, 12(10), 372; https://doi.org/10.3390/environments12100372 - 9 Oct 2025
Abstract
Xylella fastidiosa was first detected in Portugal in 2019 in Lavandula dentata. In response, the national plant health authorities promptly established a Demarcated Zone in the affected area and implemented a series of eradication and control measures, including the systematic removal and [...] Read more.
Xylella fastidiosa was first detected in Portugal in 2019 in Lavandula dentata. In response, the national plant health authorities promptly established a Demarcated Zone in the affected area and implemented a series of eradication and control measures, including the systematic removal and destruction of infected and host plants. This study analyzes the economic and operational impacts of these eradication efforts in the northern region of Portugal, with a focus on Demarcated Zones such as the Porto Metropolitan Area, Sabrosa, Alijó, Baião, Mirandela, Mirandela II, and Bougado between 2019 and June 2023. During this period, about 412,500 plants were uprooted. The majority were Pteridium aquilinum (bracken fern), with 360,324 individuals (87.3%), reflecting its wide distribution and the large area affected. Olea europaea (olive tree) was the second most common species removed, with 7024 plants (1.7%), highlighting its economic relevance. Other notable species included Quercus robur (3511; 0.85%), Pelargonium graveolens (3509; 0.85%), and Rosa spp. (1106; 0.27%). Overall, destruction costs were estimated at about EUR 1.04 million, with replanting costs of roughly EUR 6.81 million. In parallel, prospection activities—conducted to detect early signs of infection and monitor disease spread—generated expenses of roughly EUR 5.94 million. While prospecting represents a significant financial investment, the results show that it is considerably more cost-effective than large-scale eradication. Prospection enables early detection and containment, preventing the widespread destruction of vegetation and minimizing disruption to agricultural production, biodiversity, and local communities. Importantly, our findings reveal a sharp decline in confirmed cases in the initial outbreak area—the Porto Demarcated Zone—from 124 cases in 2019 to just 5 in 2023, indicating the effectiveness of the eradication and monitoring measures implemented. However, the presence of 20 active Demarcated Zones across the country as of 2023 highlights the continued risk of spread and the need for sustained vigilance. The complexity of managing Xylella fastidiosa across ecologically and logistically diverse territories justifies the high costs associated with surveillance and targeted interventions. This study reinforces the strategic value of prospection as a proactive and sustainable tool for plant health management. Effective surveillance requires the integration of advanced methodologies aligned with the phenological stages of host plants and the biological cycle of vectors. Targeting high-risk locations, optimizing sample numbers, ensuring diagnostic accuracy, and maintaining continuous training for field teams are critical for improving efficiency and reducing costs. Ultimately, the findings underscore the need to refine and adapt monitoring and eradication strategies to contain the pathogen, safeguard agricultural systems, and prevent Xylella fastidiosa from becoming endemic in Portugal. Full article
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21 pages, 2925 KB  
Review
Tree Endotherapy: A Comprehensive Review of the Benefits and Drawbacks of Trunk Injection Treatments in Tree Care and Protection
by Alessandra Benigno, Chiara Aglietti, Viola Papini, Mario Riolo, Santa Olga Cacciola and Salvatore Moricca
Plants 2025, 14(19), 3108; https://doi.org/10.3390/plants14193108 - 9 Oct 2025
Abstract
Tree endotherapy has risen to prominence in the field of precision agriculture as an innovative and sustainable method of tree care, being respectful of both environmental protection and consumer health needs. A comprehensive review of the state of the art of research in [...] Read more.
Tree endotherapy has risen to prominence in the field of precision agriculture as an innovative and sustainable method of tree care, being respectful of both environmental protection and consumer health needs. A comprehensive review of the state of the art of research in this field has made it possible to spotlight the main advantages of tree infusion, which has undergone significant progress in step with technological innovation and an increased understanding of tree anatomy and physiology. The major criticalities associated with this technique, as well as the biological and technical–operational obstacles that still hinder its wider use, are also highlighted. What emerges is an innovative and rapidly expanding technique in tree care, in both the cultivation and phytosanitary management of fruit and ornamental trees. Some of the strengths of the endotherapy technique, such as the next-to-no water consumption, the strong reduction in the use of fertilizers and pesticides, the possibility of using biological control agents (BCAs) or other products of natural origin, the precision administration of the product inside the xylem of the tree, and the efficacy (20–90%) and persistence (1–2 years) of treatments, make it one of the cornerstones of sustainable tree protection at present. With a very low consumption of the “active ingredient”, endotherapy has a negligible impact on the external environment, minimizing the drift and dispersal of the active ingredient and thus limiting the exposure of non-target organisms such as beneficial insects, birds, and wildlife. The large-scale application of the technique would therefore also help to achieve an important goal in “climate-smart agriculture”, the saving of water resources, significantly contributing to climate change mitigation, especially in those areas of the planet where water is a precious resource. Full article
(This article belongs to the Section Plant Protection and Biotic Interactions)
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20 pages, 1670 KB  
Article
Exploring Bone Health Determinants in Youth Athletes Using Supervised and Unsupervised Machine Learning
by Nikolaos-Orestis Retzepis, Alexandra Avloniti, Christos Kokkotis, Theodoros Stampoulis, Dimitrios Balampanos, Dimitrios Draganidis, Anastasia Gkachtsou, Marietta Grammenou, Anastasia Maria Karaiskou, Danai Kelaraki, Maria Protopapa, Dimitrios Pantazis, Maria Emmanouilidou, Panagiotis Aggelakis, Nikolaos Zaras, Ilias Smilios, Ioannis G. Fatouros, Maria Michalopoulou and Athanasios Chatzinikolaou
Dietetics 2025, 4(4), 44; https://doi.org/10.3390/dietetics4040044 - 4 Oct 2025
Viewed by 152
Abstract
Background: Bone health in youth is influenced by both modifiable factors, such as nutrition and physical activity, and non-modifiable factors, such as biological maturation and heredity. Understanding how these elements interact to predict body composition may enhance the effectiveness of early interventions. Importantly, [...] Read more.
Background: Bone health in youth is influenced by both modifiable factors, such as nutrition and physical activity, and non-modifiable factors, such as biological maturation and heredity. Understanding how these elements interact to predict body composition may enhance the effectiveness of early interventions. Importantly, the integration of both supervised and unsupervised machine learning models enables a data-driven exploration of complex relationships, allowing for accurate prediction and subgroup discovery. Methods: This cross-sectional study examined 94 male athletes during the developmental period. Anthropometric, performance, and nutritional data were collected, and bone parameters were assessed using dual-energy X-ray absorptiometry (DXA). Three supervised machine learning models (Random Forest, Gradient Boosting, and Support Vector Regression) were trained to predict Total Body-Less Head (TBLH) values. Nested cross-validation assessed model performance. Unsupervised clustering (K-Means) was also applied to identify dietary intake profiles (calcium, protein, vitamin D). SHAP analysis was used for model interpretability. Results: The Random Forest model yielded the best predictive performance (R2 = 0.71, RMSE = 0.057). Weight, height, and handgrip strength were the most influential predictors. Clustering analysis revealed two distinct nutritional profiles; however, t-tests showed no significant differences in TBLH or regional BMD between the clusters. Conclusions: Machine learning, both supervised for accurate prediction and unsupervised for nutritional subgroup discovery, provides a robust, interpretable framework for assessing adolescent bone health. While dietary intake clusters did not align with significant differences in bone parameters, this finding underscores the multifactorial nature of skeletal development and highlights areas for further exploration. Full article
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15 pages, 1974 KB  
Article
A Flexible Electrochemical Sensor Based on Porous Ceria Hollow Microspheres Nanozyme for Sensitive Detection of H2O2
by Jie Huang, Xuanda He, Shuang Zou, Keying Ling, Hongying Zhu, Qijia Jiang, Yuxuan Zhang, Zijian Feng, Penghui Wang, Xiaofei Duan, Haiyang Liao, Zheng Yuan, Yiwu Liu and Jinghua Tan
Biosensors 2025, 15(10), 664; https://doi.org/10.3390/bios15100664 - 2 Oct 2025
Viewed by 399
Abstract
The development of cost-effective and highly sensitive hydrogen peroxide (H2O2) biosensors with robust stability is critical due to the pivotal role of H2O2 in biological processes and its broad utility across various applications. In this work, [...] Read more.
The development of cost-effective and highly sensitive hydrogen peroxide (H2O2) biosensors with robust stability is critical due to the pivotal role of H2O2 in biological processes and its broad utility across various applications. In this work, porous ceria hollow microspheres (CeO2-phm) were synthesized using a solvothermal synthesis method and employed in the construction of an electrochemical biosensor for H2O2 detection. The resulting CeO2-phm featured a uniform pore size centered at 3.4 nm and a high specific surface area of 168.6 m2/g. These structural attributes contribute to an increased number of active catalytic sites and promote efficient electrolyte penetration and charge transport, thereby enhancing its electrochemical sensing performance. When integrated into screen-printed carbon electrodes (CeO2-phm/cMWCNTs/SPCE), the CeO2-phm/cMWCNTs/SPCE-based biosensor exhibited a wide linear detection range from 0.5 to 450 μM, a low detection limit of 0.017 μM, and a high sensitivity of 2070.9 and 2161.6 μA·mM−1·cm−2—surpassing the performance of many previously reported H2O2 sensors. In addition, the CeO2-phm/cMWCNTs/SPCE-based biosensor possesses excellent anti-interference performance, repeatability, reproducibility, and stability. Its effectiveness was further validated through successful application in real sample analysis. Hence, CeO2-phm with solvothermal synthesis has great potential applications as a sensing material for the quantitative determination of H2O2. Full article
(This article belongs to the Special Issue Advances in Nanozyme-Based Biosensors)
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23 pages, 2040 KB  
Review
Soil Properties, Processes, Ecological Services and Management Practices of Mediterranean Riparian Systems
by Pasquale Napoletano, Noureddine Guezgouz, Lorenza Parato, Rosa Maisto, Imen Benradia, Sarra Benredjem, Teresa Rosaria Verde and Anna De Marco
Sustainability 2025, 17(19), 8843; https://doi.org/10.3390/su17198843 - 2 Oct 2025
Viewed by 231
Abstract
Riparian zones, located at the interface between terrestrial and aquatic systems, are among the most dynamic and ecologically valuable landscapes. These transitional areas play a pivotal role in maintaining environmental health by supporting biodiversity, regulating hydrological processes, filtering pollutants, and stabilizing streambanks. At [...] Read more.
Riparian zones, located at the interface between terrestrial and aquatic systems, are among the most dynamic and ecologically valuable landscapes. These transitional areas play a pivotal role in maintaining environmental health by supporting biodiversity, regulating hydrological processes, filtering pollutants, and stabilizing streambanks. At the core of these functions lie the unique characteristics of riparian soils, which result from complex interactions between water dynamics, sedimentation, vegetation, and microbial activity. This paper provides a comprehensive overview of the origin, structure, and functioning of riparian soils, with particular attention being paid to their physical, chemical, and biological properties and how these properties are shaped by periodic flooding and vegetation patterns. Special emphasis is placed on Mediterranean riparian environments, where marked seasonality, alternating wet–dry cycles, and increasing climate variability enhance both the importance and fragility of riparian systems. A bibliographic study, covering 25 years (2000–2025), was carried out through Scopus and Web of Science. The results highlight that riparian areas are key for carbon sequestration, nutrient retention, and ecosystem connectivity in water-limited regions, yet they are increasingly threatened by land use change, water abstraction, pollution, and biological invasions. Climate change exacerbates these pressures, altering hydrological regimes and reducing soil resilience. Conservation requires integrated strategies that maintain hydrological connectivity, promote native vegetation, and limit anthropogenic impacts. Preserving riparian soils is therefore fundamental to sustain ecosystem services, improve water quality, and enhance landscape resilience in vulnerable Mediterranean contexts. Full article
(This article belongs to the Section Sustainability, Biodiversity and Conservation)
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12 pages, 980 KB  
Article
HS-SPME-GC-MS Volatile Profile of “Aglio Rosso di Sulmona” (Sulmona Red Garlic) Floral Scape
by Samantha Reale, Rossella Ferretti, Alessandra Biancolillo, Valter Di Cecco, Luciano Di Martino, Marco Di Santo and Angelo Antonio D’Archivio
Chemosensors 2025, 13(10), 361; https://doi.org/10.3390/chemosensors13100361 - 2 Oct 2025
Viewed by 243
Abstract
Garlic (Allium Sativum L.) is a source of organosulphur compounds with well-known sensorial and biological activity. Organosulphur precursors of garlic aroma are also detected in the plant leaves, but limited literature on this subject is available. This study is aimed at the [...] Read more.
Garlic (Allium Sativum L.) is a source of organosulphur compounds with well-known sensorial and biological activity. Organosulphur precursors of garlic aroma are also detected in the plant leaves, but limited literature on this subject is available. This study is aimed at the characterization of the volatile profile of the floral scapes of Sulmona red garlic (aglio rosso di Sulmona) cultivated in the Abruzzo region (Italy). Floral scapes are manually removed from the plant before flowering and used as an ingredient of local gastronomy. The organosulphur volatile profile of the scapes is investigated by HS-SPME-GC-MS and compared to that provided by the clove. The GC-MS chromatogram of garlic clove, which is characterized by the predominant contribution of a few organosulphur organic compounds, is significantly more intense than that of the scapes. Almost all the organosulphur compounds contributing to the clove aroma were detected in the scape volatile profile, which, however, exhibits a more balanced contribution of major and minor organo sulphur compounds. Moreover, a significantly higher relative abundance of terpenes and aldehydes is observed in the scape aroma. The geographical/varietal origin of clove seeds (Sulmona versus Spain or France) and cultivation area interactively influence the aroma profile of Sulmona red garlic scapes. Full article
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26 pages, 10152 KB  
Article
Linking Acoustic Indices to Vegetation and Microclimate in a Historical Urban Garden: Setting the Stage for a Restorative Soundscape
by Alessia Portaccio, Francesco Chianucci, Francesco Pirotti, Marco Piragnolo, Marco Sozzi, Andrea Zangrossi, Miriam Celli, Marta Mazzella di Bosco, Monica Bolognesi, Enrico Sella, Maurizio Corbetta, Francesca Pazzaglia and Raffaele Cavalli
Land 2025, 14(10), 1970; https://doi.org/10.3390/land14101970 - 30 Sep 2025
Viewed by 338
Abstract
Urban soundscapes are increasingly recognized as fundamental for both ecological integrity and human well-being, yet the complex interplay between the vegetation structure, seasonal dynamics, and microclimatic factors in shaping these soundscapes remains poorly understood. This study tests the hypothesis that vegetation structure and [...] Read more.
Urban soundscapes are increasingly recognized as fundamental for both ecological integrity and human well-being, yet the complex interplay between the vegetation structure, seasonal dynamics, and microclimatic factors in shaping these soundscapes remains poorly understood. This study tests the hypothesis that vegetation structure and seasonally driven biological activity mediate the balance and the quality of the urban acoustic environment. We investigated seasonal and spatial variations in five acoustic indices (NDSI, ACI, AEI, ADI, and BI) within a historical urban garden in Castelfranco Veneto, Italy. Using linear mixed-effects models, we analyzed the effects of season, microclimatic variables, and vegetation characteristics on soundscape composition. Non-parametric tests were used to assess spatial differences in vegetation metrics. Results revealed strong seasonal patterns, with spring showing increased NDSI (+0.17), ADI (+0.22), and BI (+1.15) values relative to winter, likely reflecting bird breeding phenology and enhanced biological productivity. Among microclimatic predictors, temperature (p < 0.001), humidity (p = 0.014), and solar radiation (p = 0.002) showed significant relationships with acoustic indices, confirming their influence on both animal behaviour and sound propagation. Spatial analyses showed significant differences in acoustic patterns across points (Kruskal–Wallis p < 0.01), with vegetation metrics such as tree density and evergreen proportion correlating with elevated biophonic activity. Although the canopy height model did not emerge as a significant predictor in the models, the observed spatial heterogeneity supports the role of vegetation in shaping urban sound environments. By integrating ecoacoustic indices, LiDAR-derived vegetation data, and microclimatic parameters, this study offers novel insights into how vegetational components should be considered to manage urban green areas to support biodiversity and foster acoustically restorative environments, advancing the evidence base for sound-informed urban planning. Full article
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21 pages, 8988 KB  
Article
Investigation of the Substrate Selection Mechanism of Poly (A) Polymerase Based on Molecular Dynamics Simulations and Markov State Model
by Yongxin Jiang, Xueyan Duan, Jingxian Zheng, Fuyan Cao, Linlin Zeng and Weiwei Han
Int. J. Mol. Sci. 2025, 26(19), 9512; https://doi.org/10.3390/ijms26199512 - 29 Sep 2025
Viewed by 248
Abstract
RNA polymerases are essential enzymes that catalyze DNA transcription into RNA, vital for protein synthesis, gene expression regulation, and cellular responses. Non-template-dependent RNA polymerases, which synthesize RNA without a template, are valuable in biological research due to their flexibility in producing RNA without [...] Read more.
RNA polymerases are essential enzymes that catalyze DNA transcription into RNA, vital for protein synthesis, gene expression regulation, and cellular responses. Non-template-dependent RNA polymerases, which synthesize RNA without a template, are valuable in biological research due to their flexibility in producing RNA without predefined sequences. However, their substrate polymerization mechanisms are not well understood. This study examines Poly (A) polymerase (PAP), a nucleotide transferase superfamily member, to explore its substrate selectivity using computational methods. Previous research shows PAP’s polymerization efficiency for nucleoside triphosphates (NTPs) ranks ATP > GTP > CTP > UTP, though the reasons remain unclear. Using 500 ns Gaussian accelerated molecular dynamics simulations, stability analysis, secondary structure analysis, MM-PBSA calculations, and Markov state modeling, we investigate PAP’s differential polymerization efficiencies. Results show that ATP binding enhances PAP’s structural flexibility and increases solvent-accessible surface area, likely strengthening protein–substrate or protein–solvent interactions and affinity. In contrast, polymerization of other NTPs leads to a more open conformation of PAP’s two domains, facilitating substrate dissociation from the active site. Additionally, ATP binding induces a conformational shift in residues 225–230 of the active site from a loop to an α-helix, enhancing regional rigidity and protein stability. Both ATP and GTP form additional π–π stacking interactions with PAP, further stabilizing the protein structure. This theoretical study of PAP polymerase’s substrate selectivity mechanisms aims to clarify the molecular basis of substrate recognition and selectivity in its catalytic reactions. These findings offer valuable insights for the targeted engineering and optimization of polymerases and provide robust theoretical support for developing novel polymerases for applications in drug discovery and related fields. Full article
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15 pages, 5911 KB  
Article
Integrative Bioinformatics-Guided Analysis of Glomerular Transcriptome Implicates Potential Therapeutic Targets and Pathogenesis Mechanisms in IgA Nephropathy
by Tiange Yang, Mengde Dai, Fen Zhang and Weijie Wen
Bioengineering 2025, 12(10), 1040; https://doi.org/10.3390/bioengineering12101040 - 27 Sep 2025
Viewed by 352
Abstract
(1) Background: IgA nephropathy (IgAN) is a leading cause of chronic kidney disease worldwide. Despite its prevalence, the molecular mechanisms of IgAN remain poorly understood, partly due to limited research scale. Identifying key genes involved in IgAN’s pathogenesis is critical for novel diagnostic [...] Read more.
(1) Background: IgA nephropathy (IgAN) is a leading cause of chronic kidney disease worldwide. Despite its prevalence, the molecular mechanisms of IgAN remain poorly understood, partly due to limited research scale. Identifying key genes involved in IgAN’s pathogenesis is critical for novel diagnostic and therapeutic strategies. (2) Methods: We identified differentially expressed genes (DEGs) by analyzing public datasets from the Gene Expression Omnibus. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to elucidate the biological roles of DEGs. Hub genes were screened using weighted gene co-expression network analysis combined with machine learning algorithms. Immune infiltration analysis was conducted to explore associations between hub genes and immune cell profiles. The hub genes were validated using receiver operating characteristic curves and area under the curve. (3) Results: We identified 165 DEGs associated with IgAN and revealed pathways such as IL-17 signaling and complement and coagulation cascades, and biological processes including response to xenobiotic stimuli. Four hub genes were screened: three downregulated (FOSB, SLC19A2, PER1) and one upregulated (SOX17). The AUC values for identifying IgAN in the training and testing set ranged from 0.956 to 0.995. Immune infiltration analysis indicated that hub gene expression correlated with immune cell abundance, suggesting their involvement in IgAN’s immune pathogenesis. (4) Conclusion: This study identifies FOSB, SLC19A2, PER1, and SOX17 as novel hub genes with high diagnostic accuracy for IgAN. These genes, linked to immune-related pathways such as IL-17 signaling and complement activation, offer promising targets for diagnostic development and therapeutic intervention, enhancing our understanding of IgAN’s molecular and immune mechanisms. Full article
(This article belongs to the Special Issue Advanced Biomedical Signal Communication Technology)
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17 pages, 5442 KB  
Article
Solid-State Fermentation of Riceberry Rice with Mushroom Mycelium for Enhanced Beta-Glucan Production and Health Applications
by Jutamat Nacha, Hongyu Chen, Amorn Owatworakit, Kittirat Saharat, Anupong Makeudom and Sunita Chamyuang
Molecules 2025, 30(19), 3879; https://doi.org/10.3390/molecules30193879 - 25 Sep 2025
Viewed by 564
Abstract
Beta-glucans (β-glucans), polysaccharides found in cereals and fungi, are recognized for their prebiotic and potential anti-cancer activities, particularly in the colorectal area. This study aims to optimize the production of β-glucan through the solid-state fermentation of germinated Riceberry rice with Pleurotus ostreatus and [...] Read more.
Beta-glucans (β-glucans), polysaccharides found in cereals and fungi, are recognized for their prebiotic and potential anti-cancer activities, particularly in the colorectal area. This study aims to optimize the production of β-glucan through the solid-state fermentation of germinated Riceberry rice with Pleurotus ostreatus and evaluate the bioactivities of the resulting extract. The crude β-glucan extract, obtained with a recovery rate of 54.95% and 79.98% purity, demonstrated an effective extraction process, as confirmed by thermogravimetric analysis (TGA). Fourier-transform infrared spectroscopy (FTIR) analysis verified the presence of β-1,3/1,6-glycosidic linkages, characteristic of the bioactive β-glucans found in yeast and mushrooms. The biological assessment demonstrated the extract’s functional properties. At a concentration of 1 mg/mL, the crude β-glucan extract significantly promoted the growth of probiotics Lacticaseibacillus rhamnosus and Bacillus coagulans, exhibiting high Prebiotic Index (PI) values of 6.36 ± 0.72 and 115.70 ± 10.19, respectively, with PI values indicating strong prebiotic potential. For comparison, the standard prebiotic inulin yielded PI values of 0.41 ± 0.09 and 90.53 ± 2.28 for the same respective bacteria, highlighting the superior performance of the fungal-fermented β-glucan. Furthermore, the extract displayed efficacy in inhibiting colon cancer cells in preliminary in vitro tests. It reduced the viability of the SW480 colorectal cancer cell line by 66.23% and induced cell death in 27.94 ± 0.93% of the cells after 48 h of treatment, performing comparably to a commercial yeast β-glucan standard. Crucially, the extract showed no significant cytotoxicity toward the normal human colon cell line, CCD-841 CoN. These findings highlight the promising method of fungal solid-state fermentation on germinated Riceberry rice in the production of high-purity, bioactive β-glucans for use in functional foods. Full article
(This article belongs to the Special Issue New Development in Fermented Products—Third Edition)
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15 pages, 6815 KB  
Article
Structural Characterization, Cytotoxicity and Microbiological Activity of One-Step-Synthesized RGO/AuNPs Nanocomposites
by Boris Martinov, Dimitar Dimitrov, Tsvetelina Foteva, Aneliya Kostadinova and Anna Staneva
Materials 2025, 18(19), 4464; https://doi.org/10.3390/ma18194464 - 25 Sep 2025
Viewed by 242
Abstract
This study presents a green, single-step method for synthesizing nanocomposites based on reduced graphene oxide (RGO) and gold nanoparticles (AuNPs), using sodium citrate as a mild reducing and stabilizing agent. AuNPs were generated from chloroauric acid (HAuCl4) directly on the surface [...] Read more.
This study presents a green, single-step method for synthesizing nanocomposites based on reduced graphene oxide (RGO) and gold nanoparticles (AuNPs), using sodium citrate as a mild reducing and stabilizing agent. AuNPs were generated from chloroauric acid (HAuCl4) directly on the surface of graphene oxide (GO), which was simultaneously reduced to RGO. Structural characterization via Transmission Electron Microscopy (TEM), High Resolution TEM (HRTEM) and Selected Area Electron Diffraction (SAED) confirms spherical AuNPs (10–60 nm) distributed on RGO sheets, with indications of nanoparticle aggregation. Dynamic Light Scattering (DLS) and zeta potential analysis support these findings, suggesting colloidal instability with higher RGO content. Biological evaluation demonstrates dose-dependent cytotoxicity in HaCaT keratinocytes, with IC50 values (half maximal inhibitory concentration) decreasing as RGO content is increased. At moderate dilutions (1–25 µL/100 µL), the composites show acceptable cell viability (>70%). Antibacterial assays reveal strong synergistic effects against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis, with sample RGO/Au 0.500/0.175 g/L showing complete E. coli inhibition at low Au content (0.175 g/L). The composite retained activity even in protein-rich media, suggesting potential for antimicrobial applications. These findings highlight the potential of RGO/AuNPs composites as multifunctional materials for biomedical uses, particularly in antimicrobial coatings and targeted therapeutic strategies. Full article
(This article belongs to the Section Electronic Materials)
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17 pages, 1741 KB  
Article
Fabrication and Characterization of Curcumin-Complexed Nanoparticles Using Coconut Protein Nanoparticles
by Leila Ziaeifar, Maryam Salami, Gholamreza Askari, Zahra Emam-Djomeh, Raimar Loebenberg, Michael J Serpe and Neal M. Davies
Pharmaceutics 2025, 17(10), 1247; https://doi.org/10.3390/pharmaceutics17101247 - 24 Sep 2025
Viewed by 316
Abstract
Background/Objectives: Curcumin (Cur) has various biological properties, including anti-microbial, antioxidant, anticancer, anti-diabetic, anticarcinogenic, antitumor, and anti-inflammatory activities. However, using Cur in functional food products is challenging because of its low solubility in an aqueous environment, rapid degradation, and low bioavailability. Nanostructure delivery [...] Read more.
Background/Objectives: Curcumin (Cur) has various biological properties, including anti-microbial, antioxidant, anticancer, anti-diabetic, anticarcinogenic, antitumor, and anti-inflammatory activities. However, using Cur in functional food products is challenging because of its low solubility in an aqueous environment, rapid degradation, and low bioavailability. Nanostructure delivery systems provide a high surface area to volume ratio and sustainable release properties. Methods: Coconut protein nanoparticles (CPNPs) have been fabricated through heat treatment at 85 °C and pH 2 for 5 h. The formation of CPNP-Cur was used to improve Cur solubility, followed by antioxidant activity at neutral pH in an aqueous solution. Results: The maximum efficiency and loading capacity of Cur in CPNP were 96.6% and 19.32 µg/mg protein, respectively. Scanning electron microscopy indicated the spherical and organized shape of CPNP with a small size of 80 nm. The fluorescence quenching of CPNP-Cur confirmed the potential of Cur to bind to the tryptophane and tyrosine residues in CPNP. The structural properties of CPNP and CPNP-Cur were investigated using FTIR and X-ray diffraction. The antioxidant activity of samples, measured with the ABTS radical scavenging method, demonstrated that the antioxidant capacity of the aqueous solution of Cur was significantly enhanced through the encapsulation into CPNP. The steady release of Cur was observed in the simulated gastrointestinal tract, and the percentage of the cumulative release increased up to 29.2% after 4 h. Conclusions: Our findings suggest that CPNP was a suitable nanocarrier for Cur due to improved antioxidant activity and controlled release behavior. These results are valuable for the development of coconut protein nanoparticles to use as a novel nano-delivery system of bioactive components. Full article
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24 pages, 11361 KB  
Article
Analysis of Exposure to ALAN (Artificial Light at Night) in the Urban Space of Madrid and Toledo (Spain) and Its Impact on Human Circadian Rhythms: “Circadian Neurolighting”
by Nubi Leon Martinez and Mercedes Valiente Lopez
Urban Sci. 2025, 9(9), 386; https://doi.org/10.3390/urbansci9090386 - 22 Sep 2025
Viewed by 595
Abstract
This research explores the visual and non-visual (circadian) perception of light in humans and their brain responses in the urban spaces of two contrasted cities: Madrid and Toledo, in Spain. Lighting has been demonstrated to induce the synthesis of neurotransmitters, which can function [...] Read more.
This research explores the visual and non-visual (circadian) perception of light in humans and their brain responses in the urban spaces of two contrasted cities: Madrid and Toledo, in Spain. Lighting has been demonstrated to induce the synthesis of neurotransmitters, which can function as regulators or disruptors of our biological system, causing diseases due to the alteration in our circadian rhythms; these responses are contingent upon the technical properties of the lighting (type of incidence, intensity, colour temperature, and primarily, wavelength), as well as the time and duration of exposure. In Madrid, we analyse Gran Vía, an iconic and busy avenue with high commercial and touristic activity. This has resulted in an excess of illumination, which has a notable impact on the night environment and, in particular, on the biology of the human beings who transit and inhabit these areas. In contrast to Toledo—a small, protected city (a UNESCO World heritage site since 1986) that has maintained a low population density, controlled commercial areas, and a low level of urban lighting at night—Madrid represents an opposite situation. In Madrid, measurements of both lux and light spectrum were taken to demonstrate the excessive light incidence, along with the high emission of short-wavelength light produced by LED screens, which, at night, disrupt our circadian cycle. This paper demonstrates how artificial light at night (ALAN) affects human circadian rhythms. Furthermore, this study suggests directions for urban lighting design, considering human circadian rhythms. Full article
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41 pages, 1997 KB  
Review
COMP Is a Biomarker of Cartilage Destruction, Extracellular Matrix and Vascular Remodeling and Tissue Repair
by Margaret M. Smith and James Melrose
Int. J. Mol. Sci. 2025, 26(18), 9182; https://doi.org/10.3390/ijms26189182 - 19 Sep 2025
Viewed by 789
Abstract
This review covers the roles of cartilage oligomeric matrix protein (COMP), an established biomarker of cartilage breakdown in pathological tissues in osteoarthritis, and in emerging areas in extracellular matrix and vascular remodeling associated with trauma, fibrosis and cancer. COMP is produced by chondrocytes, [...] Read more.
This review covers the roles of cartilage oligomeric matrix protein (COMP), an established biomarker of cartilage breakdown in pathological tissues in osteoarthritis, and in emerging areas in extracellular matrix and vascular remodeling associated with trauma, fibrosis and cancer. COMP is produced by chondrocytes, tenocytes, myofibroblasts, and in some specialized tissue contexts, endothelial and vascular smooth muscle cells. COMP expression by tendon and cartilage cells is sensitive to weight bearing and tensional mechanical stimulation. Vascular smooth muscle cells are sensitive to shear forces which regulate COMP expression in vascular tissues in atherosclerosis and in carotid stenosis. COMP is a multivalent bridging molecule that stabilizes tissues. It facilitates the signaling of TGF-β and BMP-2 in chondrogenesis, osteogenesis, tissue fibrosis, vascular and ECM remodeling and tumor development by providing a multimeric environment through which growth factor binding and receptor activation can occur. Engineered COMP proteins have been used as molecular templates in the development of chimeric therapeutic proteins of potential application in repair biology. Tie2 (Angiopoietin-1 receptor, Tyrosine-protein kinase receptor TEK), when activated by an engineered COMP-inspired angiopoietin-2 pentamer, is a potent angiogenic molecule of obvious application in wound healing. COMP’s multifunctional properties show it is much more than a biomolecular marker protein through its ability to participate in many biological processes. Further studies are warranted to fully explore the biology of this fascinating molecule, particularly in the wound repair processes. Full article
(This article belongs to the Special Issue Molecular Research on Osteogenesis)
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