Search Results (23,592)

This study aimed to investigate the molecular mechanisms underlying dopaminergic injury induced by gestational and lactational atrazine (ATR) exposure in rat offspring, with a particular focus on non-coding RNA-mediated regulation. Pregnant rats were exposed to ATR during gestation and lactation. Offspring underwent behavioral testing at postnatal day 21 (PND21) and were sacrificed for midbrain tissue collection at PND28. Behavioral alterations, histopathological changes in the substantia nigra, and dopaminergic marker expression were assessed to evaluate ATR-induced neurotoxicity. Whole-transcriptome sequencing was then performed to identify differentially expressed mRNAs, miRNAs, and lncRNAs, followed by co-expression, protein–protein interaction, and competing endogenous RNA (ceRNA) network analyses. Key targets were validated by qRT-PCR. Candidate molecules identified from transcriptomic and ceRNA analyses were further examined in an ATR-induced neurotoxicity model established in RA-differentiated SK-N-SH cells. Dual-luciferase reporter, Ago2-RNA immunoprecipitation, and biotin-labeled RNA pull-down assays were used to examine putative binding relationships and molecular interactions. In addition, lentivirus-mediated Elavl4 overexpression was performed to further evaluate the role of this candidate regulator in ATR-induced Nurr1 downregulation. Gestational and lactational ATR exposure induced significant behavioral abnormalities in rat offspring. These changes were accompanied by histopathological alterations in the substantia nigra, including reduced TH immunoreactivity, as well as abnormal expression of dopaminergic markers, characterized by decreased TH and Nurr1 levels and increased α-syn expression. Together, these findings indicate the presence of dopaminergic injury. Whole-transcriptome analysis further revealed widespread dysregulation of mRNAs, miRNAs, and lncRNAs in ATR-exposed offspring. Subsequent integrative analysis suggested a potential ceRNA regulatory relationship among Elavl4, miR-301a-5p, and Nurr1, which was further supported by qRT-PCR. Dual-luciferase reporter, RIP, and RNA pull-down assays supported direct interactions between miR-301a-5p and both Elavl4 and Nurr1, as well as their association with the Ago2-containing silencing complex. Moreover, Elavl4 overexpression partially reversed ATR-induced Nurr1 downregulation in vitro. Gestational and lactational ATR exposure induced behavioral abnormalities and dopaminergic injury in rat offspring. Whole-transcriptome analysis combined with experimental validation suggests a potential association between the Elavl4/miR-301a-5p/Nurr1 ceRNA axis and ATR-induced dopaminergic injury, providing insight into the post-transcriptional mechanisms underlying developmental neurotoxicity. Full article

Internet Gaming Disorder (IGD) has emerged as an increasingly prevalent behavioral health concern among late adolescent university students, a vulnerable population with emotional distress due to the developmental changes and academic pressures. This research explored the direct correlations between IGD and Mental Health Disorder (MHD), such as depression, anxiety, and stress in Saudi Arabia (SA) with study discipline as a moderator. A total of 480 students participated in the developed self-structured questionnaire, and Partial Least Squares Structural Equation Modeling (PLS-SEM) was employed to analyze the obtained data. The results showed that IGD can exert a positive and significant association with all three aspects of MHD. Moreover, the PLS-SEM slope analysis indicated that study discipline can significantly moderate the link from IGD to both anxiety and depression, with university students in health, science, and engineering fields displaying higher symptoms of depression and anxiety as compared to their peers in humanities and social sciences. However, study discipline failed to moderate the link from IGD to stress. These findings can be interpreted through maladaptive coping mechanisms and behavioral addiction, whereby extreme IG can contribute to social withdrawal, reduce sleep quality, and worsen stress regulation, specifically during the late adolescence period. The results extend current research on IGD by emphasizing the disciplinary differences in mental health vulnerability and offering more empirical evidence from a Middle Eastern context. The study highlighted the urgent need for discipline-oriented mental health screening and targeted proactive interventions to deal with unsettled IG attitude within a higher education context. Full article

SiO₂ aerogel cement composites (SACCs) are promising for building insulation, but how their residual thermal performance evolves after high-temperature exposure remains unclear, limiting fire protection assessment. In this study, SACC specimen with aerogel contents of 0%, 5%, 7%, and 10% were heat-treated at 400, 600, 700, 800, and 1000 °C. After cooling, their post-exposure thermal performance and microstructure were characterized via mass loss, density, thermal conductivity, MIP, and SEM. Results obtained at room temperature showed that with increasing treatment temperature, thermal conductivity first decreases and then increases, reaching a minimum after 700 °C treatment for the A7 specimens (from 0.092 to 0.063 W/(m·K)). Microstructural analysis of cooled specimens revealed that this non-monotonic behavior arises from three heat-induced changes: the cement matrix, aerogel aggregates, and the interfacial gap between them. After treatment at 700 °C, the gap corresponds to a Knudsen number of 0.01–0.02, entering the slip-flow regime. Combined with the low thermal conductivity of the cement matrix, this yields the best insulation. After treatment at 800 °C and above, the gap exceeded 60 μm, shifting heat transfer to the continuum regime and reducing insulation capacity. A thermal conductivity prediction model based on these post-exposure mechanisms agreed well with the experimental results. Full article

Atmospheric heatwaves (AHWs) and marine heatwaves (MHWs) are intensifying under climate change, yet their coupled behavior in the Red Sea remains insufficiently quantified. This study investigates the spatial and temporal characteristics of AHWs, MHWs, and their concurrent occurrence across the Red Sea from 1990 to 2024 using ERA5 surface air temperature (SAT) and NOAA OISST v2.1 satellite-derived sea surface temperature (SST). Remote-sensing daily satellite-derived Level-4 (L4) OISST products were used in this study to enable spatially complete and temporally consistent detection of MHWs in this narrow, semi-enclosed basin despite contamination and coastal sampling constraints. Both SAT and SST exhibit statistically significant warming trends (p < 0.05), with basin mean increases of 0.40 ± 0.07 °C/decade and 0.31 ± 0.05 °C/decade, respectively. The strongest warming was observed in the central and northern Red Sea. This warming is accompanied by significant increases in the frequency and duration of AHWs, MHWs, and their concurrent AHW-MHW events, particularly after 2010, indicating a shift toward more frequent heatwave conditions. AHWs occur more frequently than MHWs across the Red Sea, whereas MHWs exhibit long duration, particularly in the northern Red Sea, where annual durations exceed 45–50 days/year. Concurrent AHW-MHW events account for about 66% of MHWs in the Red Sea, and their characteristics show a significant increasing trend across the entire basin. These findings identify the Red Sea as a regional hotspot of increasing concurrent heatwave events and highlight the importance of satellite-based monitoring for assessing evolving climate risks in semi-enclosed basins. Full article

Mechanically activated pyrite plays an important role in gold extraction and coal utilization, but its reactivity may change markedly during storage. This study investigates how air deactivation during storage affects the crystal structure and subsequent thermal decomposition behavior of mechanically activated pyrite. Pyrite was mechanically activated and then stored in air for 0, 7 and 180 days. X-ray diffraction (XRD) combined with Rietveld refinement was used to characterize variations in lattice parameters and unit-cell-related structural features, while non-isothermal thermogravimetric–differential scanning calorimetry (TG-DSC) under an argon atmosphere, together with the Flynn–Wall–Ozawa (FWO) method, was applied to evaluate the decomposition kinetics. Air deactivation induced a non-monotonic evolution of lattice parameters and unit-cell volume, which is attributed to combined effects of residual stress relaxation and air-induced surface-related modification during storage. All samples exhibited two mass-loss stages during heating, reflecting stepwise thermal decomposition, and their decomposition behavior varied systematically with deactivation time. The apparent activation energy depended on both conversion fraction and deactivation degree, and nucleation-and-growth-type mechanisms were found to dominate the decomposition process, with their relative contributions evolving with storage time. These results clarify how prior air-deactivation history influences the structural evolution and subsequent thermal decomposition behavior of mechanically activated pyrite and provide useful insight for its storage and utilization in related processes. Full article

This study examined how the extraction medium used to obtain Quercus robur extracts influenced the properties of electrospun poly(vinyl alcohol) (PVA) mats intended for potential active packaging applications. Extracts prepared with 50% ethanol and with a choline chloride:lactic acid:water system were incorporated into PVA spinning solutions, and their effects on solution properties, fiber morphology, thermal behavior, crosslinking response, and polyphenol release were evaluated. The type of extraction medium affected both the electrospinning process and the structure of the resulting materials. Ethanol-derived extracts reduced solution viscosity and promoted the formation of thinner fibers, whereas systems containing the choline chloride:lactic acid:water-derived extract showed higher conductivity and lower electrospinning stability. Crosslinking with tannic acid in water led to the collapse of the fibrous structure, while ethanolic tannic acid treatment preserved the nanofibrous morphology more effectively. FTIR analysis indicated differences in intermolecular interactions within the polymer matrix, consistent with the observed changes in structural stability and release behavior. Thermal analysis showed that ethanol-derived extracts lowered the thermal stability of the PVA matrix, whereas the choline chloride:lactic acid:water-derived system altered the degradation pathway and increased the amount of solid residue formed during heating. Release studies demonstrated a rapid burst release for ethanol-based mats and a more sustained release profile for mats containing the choline chloride:lactic acid:water-derived extract. Selected extract-containing and ethanol–tannic acid-crosslinked mats also showed antibacterial activity against Staphylococcus aureus. The results showed that the extraction medium significantly affected polymer–extract interactions and the functional properties of electrospun PVA mats. At the same time, the conclusions refer specifically to the tested solvent systems, and broader generalization to other natural deep eutectic solvent-type formulations requires further comparative studies. Full article

This work establishes a map between deposition, structure, and properties that enables the design of Ni-P coatings for advanced surface engineering applications. The coatings were electrodeposited on 316L stainless steel substrates using electrolytes of different phosphorus contents, achieved by systematically varying the phosphorous acid (H₃PO₃) concentrations. The influence of phosphorus content and intrinsic pH on elemental composition, cathodic current efficiency (CCE), thickness, microstructure, surface topography, crystalline structure, optical properties, and tribological behavior was investigated. The incorporation of phosphorus follows the H₃PO₃ concentration increase in a non-linear trend, achieving a maximum value of 22.17 at.% P at the highest bath concentration. The CCE presented an opposite trend, decreasing from approximately 96% to 40%, due to intense activity of hydrogen evolution reactions, and evidencing indirect phosphorus incorporation mechanisms. A transition from crystalline to amorphous structures was observed as the phosphorus content increased, being accompanied by grain refinement and significant roughness reduction to a minimum Sa = 8 ± 1 nm at ~15 at.% P. The optical properties, such as diffuse reflectivity and CIE Lab* color coordinates, were strongly correlated to surface roughness and microstructural evolution, demonstrating the influence of phosphorus through structural changes. Tribological behavior of the coatings revealed a complex interplay between composition, roughness, and wear mechanisms. The lower and more stable coefficients of friction were observed for high phosphorus coatings, although their durability depended on the balance between brittleness and grain refinement. The results demonstrate the combined role of phosphorus concentration and intrinsic pH changes as an effective tool for tailoring the structural, optical, and tribological properties of electrodeposited Ni-P coatings. Full article

We study the long-time behavior of nonlinear stochastic evolution equations in a separable Hilbert space driven by a Q-Wiener process. The linear part of the equation is generated by a strongly continuous semigroup with an exponential dichotomy, which provides fixed rates of decay and growth. The nonlinear drift and diffusion terms are globally Lipschitz and become small as time tends to infinity. Our main result shows that under these conditions, the mean-square Lyapunov exponents of the nonlinear system coincide with those of the linear part. In other words, nonlinear stochastic perturbations that decay in time do not change the main growth or decay rates of solutions in the mean-square sense. This result provides simple and verifiable criteria ensuring that the long-time Lyapunov behavior of the nonlinear stochastic equation is fully determined by the linear semigroup, even in the presence of time-dependent stochastic perturbations. Full article

Objectives: Government surveillance of American Muslims has grown following 9/11, yet little scholarship has analyzed how this activity impacts political participation. We examine racial and ethnic variation in American Muslims’ experiences of state surveillance, as well as the connection between those experiences and voter turnout. Methods: Using a survey of 1000 American Muslims, we identify racial and ethnic patterns in being singled out in airports and by the police. We then analyze how being stopped in these venues shaped turnout behavior in the 2016 presidential election. Results: Black Muslims are more likely to encounter surveillance from the police, while Muslims who identify as Asian report the highest degree of monitoring in airports. We find that police encounters are linked to decreased electoral participation, but being singled out by airport security is not tied to a change in turnout. Conclusions: These findings provide a more detailed and comprehensive understanding of who is impacted by surveillance in the US and how that surveillance shapes American democracy. Full article

The obligate mutualism between Ficus and its pollinating wasps provides a suitable system to investigate these dynamics because it encompasses two contrasting pollination modes: active and passive. Here we compared pollen traits in an actively pollinated fig tree, Ficus citrifolia, and a passively pollinated species, F. obtusiuscula, examining pollen both at anther presentation and after deposition on the bodies of their pollinating wasps. Pollen morphology, hydration-related behavior, cytology, and reserve composition were characterized using scanning electron microscopy (conventional and modified), light and transmission electron microscopy, histochemical assays, and viability tests. Across species, pollen traits at anthesis showed broad overlap in morphology, viability and major reserve classes, indicating that these characteristics are not consistently predicted by pollination mode alone. In both species, pollen was bicellular, harmomegathic and highly viable at presentation, consistent with resilience during transport. The main divergence emerged after pollen transfer to the pollinator. In the actively pollinated species, pollen recovered from wasp thoracic pockets exhibited pronounced intracellular remodeling, including vacuolization, starch depletion, lipid redistribution and localized cytoplasmic degradation. By contrast, pollen of the passively pollinated species retained a comparatively stable cytological organization after transport despite changes in reserve distribution. These results suggest that the more pronounced cytoplasmic reorganization observed in the pollen of the actively pollinated species after deposition on the wasp body may represent a preparatory phase for rapid germination following pollination, reflecting the stronger dependence of larval development on successful flower fertilization in actively pollinated figs. More broadly, our study provides the first comparative account of pollen structural and cytophysiological dynamics on fig-wasp bodies, linking pollen cell biology to pollinator-mediated dispersal and highlighting how different pollination strategies may impose distinct selective pressures on male gametophytes. Full article

Background: In older people, syphilis diagnosis might be undervalued due to both clinical conditions and age-related changes that obscure symptom presentation and physician discomfort with sexual history-taking, creating a dual barrier to timely recognition. Methods: Case presentation with literature review. Results: An 80-year-old woman was referred to the Dermatology Department of Cagliari University by her oncologist, with a 2-month history of intermittent episodes of pruritus associated with papular–nodular skin lesion eruptions, accompanied with asthenia, night sweats, and unintentional weight loss, indicative of a paraneoplastic syndrome or an adverse drug reaction. Careful evaluation indicated the need to perform serological testing, which confirmed secondary syphilis (RPR 1:64 and TPHA 1:5120). Specific questioning regarding sexual behaviors pointed out oral and anal intercourse. The 83-year-old husband did not have active lesions at visit but reported a self-healing generalized skin rash, episodes of asthenia, arthralgia, and headache he had never suffered before. Blood tests showed positive RPR 1:64 and TPHA 1:5120. Targeted sexual history assessment disclosed patient’s engaging with commercial sex workers, clarifying the chain of transmission in this conjugal STI case. Treatment with Benzathine penicillin G 2.4 million units IM in a single dose resulted in complete recovery in both patients. Conclusions: The observation highlights the importance of maintaining a high index of suspicion for syphilis even at advanced age. Persistent stigma regarding elderly sexuality should be faced, and targeted interventions are necessary to improve the clinician’s ability to identify STIs in older adults, but also to reduce sexual stigma and taboo persistence in the general population. Full article

In the process of extracting and transporting waxy crude oil, pipeline blockages resulting from wax deposition significantly impede production efficiency and lead to substantial economic losses. Ethylene vinyl acetate copolymer (EVA) is a widely used chemical wax inhibitor; however, its performance is influenced by multiple factors, including its molecular structure, concentration, and the carbon number distribution of the wax system. A systematic elucidation of its mechanism of action and associated phase changes is therefore necessary. In this study, differential scanning calorimetry (DSC) was employed to systematically investigate the thermal behavior of a wax system with a broad carbon number distribution (C5–C50). The objectives were to analyze the influence of EVA concentration, vinyl acetate (VA) content, and molecular weight on the phase transition parameters, to elucidate the wax inhibition mechanism, and to construct a phase prediction model based on the Flory–Huggins theory. The results demonstrate that the wax appearance temperature (WAT), phase transition temperature, and phase transition enthalpy of the wax systems increase monotonically with carbon number. Furthermore, the addition of EVA was found to significantly reduce both the WAT and the amount of wax precipitated. The optimal structural parameters were identified as a VA content of 10%, a number average molecular weight of 20,000, and an optimal concentration of 800 ppm. The medium-carbon wax system (C16–C30) was found to be the most sensitive to the EVA response. The established phase model exhibited high predictive accuracy, with a mean relative error of less than 4%, a root mean square error (RMSE) of 0.32%, and a coefficient of determination (R²) of 0.987, thereby providing preliminary insights and a practical tool for optimizing EVA wax inhibitor formulations under simplified conditions and guiding their potential engineering applications. Full article

Ototoxicity represents a clinically significant complication of anticancer therapy in pediatric patients. Cytotoxic agents used in oncology, particularly platinum-based chemotherapy, may induce damage to the auditory and vestibular systems, resulting in hearing loss, tinnitus, and balance disturbances. Even mild hearing impairment during childhood may negatively affect speech perception, language development, communication abilities, and subsequent educational and psychosocial functioning. This narrative review aims to synthesize current evidence on treatment-related ototoxicity in children, with particular focus on commonly implicated therapies, clinical consequences, diagnostic approaches, and potential preventive strategies. A focused literature search was conducted in PubMed for publications from 2019 to 2025 addressing ototoxicity associated with pediatric anticancer treatment and audiological monitoring methods. The analysis indicates that platinum-based compounds, especially cisplatin and carboplatin, remain the primary agents associated with ototoxicity, with reported incidence ranging from approximately 20–70% for cisplatin and 10–30% for carboplatin. Additional risk factors include young age, baseline hearing status, renal function, and exposure to other ototoxic agents such as aminoglycoside antibiotics. Early detection relies on comprehensive audiological monitoring combining behavioral and objective methods, including pure-tone audiometry, extended high-frequency audiometry, otoacoustic emissions, and auditory brainstem response testing. Standardized grading systems such as ASHA, Brock, Chang, and SIOP Boston criteria play a key role in identifying and classifying ototoxic changes. Emerging research focuses on improved monitoring protocols, biomarker identification, and the development of otoprotective strategies, including sodium thiosulfate and experimental molecular therapies. Implementing systematic hearing monitoring and preventive strategies is essential to reduce long-term auditory complications and improve quality of life in pediatric cancer survivors. Full article

Variable cross-section cement–soil pipe piles are an innovative soft ground improvement technology. They are tubular, special-shaped cement–soil mixing piles characterized by a tapered profile along the pile shaft (larger diameter at the top and smaller at the bottom) and an internal soil core. They offer advantages including reduced material consumption, lower engineering cost, and shorter construction duration. However, the systematic theoretical understanding of their bearing performance remains insufficient. In this study, the bearing mechanism and influencing parameters of variable cross-section pipe piles were systematically investigated via full-scale field tests, numerical simulations, and laboratory model tests. An exponential decay constitutive model considering the strain-softening behavior of cement–soil was developed and implemented through secondary development in the ABAQUS platform for parametric analysis. Laboratory model tests were further conducted to advance the understanding of the bearing mechanism of variable cross-section pipe piles. The results show that the ultimate bearing capacity of the proposed variable cross-section cement–soil pipe pile is approximately 189% higher than that of the conventional ones. The expanded outer diameter and expanded height are the dominant factors affecting the bearing capacity, while the inner diameter and pile length have a comparatively minimal influence: increasing the expanded outer diameter from 0.6 m to 1.2 m and the expanded height from 0 m to 5 m increased the ultimate bearing capacity from 445 kN to 868 kN and 936 kN, respectively. The effective pile length is determined to be 6 m, and the recommended minimum wall thickness of the pipe pile is 1/4 of the inner diameter. Laboratory tests further demonstrated an abrupt change in axial force at the variable section. The findings provide reliable theoretical support for the engineering design and field application of cement–soil variable cross-section pipe piles. Full article

The increasing deployment of shared transmission corridors for High-Voltage Alternating Current (HVAC) and High-Voltage Direct Current (HVDC) systems has intensified the need to evaluate electromagnetic compatibility in hybrid overhead line configurations. This study presents an analytical methodology to estimate the electric field magnitude and magnetic flux density generated by hybrid HVAC–HVDC transmission lines under steady-state operating conditions. The electric field is determined using the Maxwell potential matrix combined with the image method, while the magnetic field is obtained from a formulation based on the Biot–Savart law. Two representative case studies were analyzed with identical electrical operating conditions but different transverse conductor arrangements to evaluate the influence of geometry on the electromagnetic environment of the corridor. The results show that variations in the spatial configuration of the conductors produce noticeable changes in the location and magnitude of the electric and magnetic field maxima across the right-of-way. These findings demonstrate that conductor geometry plays a key role in the electromagnetic behavior of hybrid corridors and should be considered in the design and assessment of HVAC–HVDC transmission systems. Full article