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20 pages, 4148 KiB  
Article
Automated Discrimination of Appearance Quality Grade of Mushroom (Stropharia rugoso-annulata) Using Computer Vision-Based Air-Blown System
by Meng Lv, Lei Kong, Qi-Yuan Zhang and Wen-Hao Su
Sensors 2025, 25(14), 4482; https://doi.org/10.3390/s25144482 - 18 Jul 2025
Abstract
The mushroom Stropharia rugoso-annulata is one of the most popular varieties in the international market because it is highly nutritious and has a delicious flavor. However, grading is still performed manually, leading to inconsistent grading standards and low efficiency. In this study, deep [...] Read more.
The mushroom Stropharia rugoso-annulata is one of the most popular varieties in the international market because it is highly nutritious and has a delicious flavor. However, grading is still performed manually, leading to inconsistent grading standards and low efficiency. In this study, deep learning and computer vision techniques were used to develop an automated air-blown grading system for classifying this mushroom into three quality grades. The system consisted of a classification module and a grading module. In the classification module, the cap and stalk regions were extracted using the YOLOv8-seg algorithm, then post-processed using OpenCV based on quantitative grading indexes, forming the proposed SegGrade algorithm. In the grading module, an air-blown grading system with an automatic feeding unit was developed in combination with the SegGrade algorithm. The experimental results show that for 150 randomly selected mushrooms, the trained YOLOv8-seg algorithm achieved an accuracy of 99.5% in segmenting the cap and stalk regions, while the SegGrade algorithm achieved an accuracy of 94.67%. Furthermore, the system ultimately achieved an average grading accuracy of 80.66% and maintained the integrity of the mushrooms. This system can be further expanded according to production needs, improving sorting efficiency and meeting market demands. Full article
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14 pages, 1435 KiB  
Article
Association Between Diet, Sociodemographic Factors, and Body Composition in Students of a Public University in Ecuador
by Angélica María Solís Manzano, María Victoria Padilla Samaniego, Verónica Patricia Sandoval Tamayo, Edgar Rolando Morales Caluña, Katherine Denisse Suarez Gonzalez, Tannia Valeria Carpio-Arias and Patricio Ramos-Padilla
Int. J. Environ. Res. Public Health 2025, 22(7), 1140; https://doi.org/10.3390/ijerph22071140 - 18 Jul 2025
Abstract
Body composition is associated with multiple factors. The main objective of this study is to determine the association between diet and sociodemographic factors on the body structure and composition of university students at a public university in Ecuador. This cross-sectional study allowed for [...] Read more.
Body composition is associated with multiple factors. The main objective of this study is to determine the association between diet and sociodemographic factors on the body structure and composition of university students at a public university in Ecuador. This cross-sectional study allowed for the collection of detailed body composition and dietary data from 204 students (41.7% men and 58.3% women, with an average age of 23.3 ± 4.4 years). The study was conducted using validated questionnaires and bioimpedance techniques. Statistical analysis included ANOVA tests, complemented by a PCA-Biplot, to examine the relationships between study variables. Statistical analysis revealed that men’s birthplace had a significant impact on several body measurements, such as hip circumference and weight, but no significant differences were observed in body structure and composition based on nutrient intake. Furthermore, larger upper-arm circumference in women was correlated with higher fat intake. The results of the multivariate analysis indicated a differential influence of dietary components on body composition. The study highlights the need for nutritional intervention strategies and educational programs that consider the diversity of students’ backgrounds to promote healthy habits and mitigate the negative effects of eating habits and irregular physical activity patterns on their health and body composition. Full article
(This article belongs to the Section Health Care Sciences)
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18 pages, 9419 KiB  
Article
STNet: Prediction of Underwater Sound Speed Profiles with an Advanced Semi-Transformer Neural Network
by Wei Huang, Junpeng Lu, Jiajun Lu, Yanan Wu, Hao Zhang and Tianhe Xu
J. Mar. Sci. Eng. 2025, 13(7), 1370; https://doi.org/10.3390/jmse13071370 - 18 Jul 2025
Abstract
The real-time acquisition of an accurate underwater sound velocity profile (SSP) is crucial for tracking the propagation trajectory of underwater acoustic signals, making it play a key role in ocean communication positioning. SSPs can be directly measured by instruments or inverted leveraging sound [...] Read more.
The real-time acquisition of an accurate underwater sound velocity profile (SSP) is crucial for tracking the propagation trajectory of underwater acoustic signals, making it play a key role in ocean communication positioning. SSPs can be directly measured by instruments or inverted leveraging sound field data. Although measurement techniques provide a good accuracy, they are constrained by limited spatial coverage and require a substantial time investment. The inversion method based on the real-time measurement of acoustic field data improves operational efficiency but loses the accuracy of SSP estimation and suffers from limited spatial applicability due to its stringent requirements for ocean observation infrastructures. To achieve accurate long-term ocean SSP estimation independent of real-time underwater data measurements, we propose a semi-transformer neural network (STNet) specifically designed for simulating sound velocity distribution patterns from the perspective of time series prediction. The proposed network architecture incorporates an optimized self-attention mechanism to effectively capture long-range temporal dependencies within historical sound velocity time-series data, facilitating an accurate estimation of current SSPs or prediction of future SSPs. Through the architectural optimization of the transformer framework and integration of a time encoding mechanism, STNet could effectively improve computational efficiency. For long-term forecasting (using the Pacific Ocean as a case study), STNet achieved an annual average RMSE of 0.5811 m/s, outperforming the best baseline model, H-LSTM, by 26%. In short-term forecasting for the South China Sea, STNet further reduced the RMSE to 0.1385 m/s, demonstrating a 51% improvement over H-LSTM. Comparative experimental results revealed that STNet outperformed state-of-the-art models in predictive accuracy and maintained good computational efficiency, demonstrating its potential for enabling accurate long-term full-depth ocean SSP forecasting. Full article
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21 pages, 625 KiB  
Article
An Effective Hybrid Sampling Strategy for Single-Split Evaluation of Classifiers
by Show-Jane Yen, Yue-Shi Lee and Yi-Jie Tang
Electronics 2025, 14(14), 2876; https://doi.org/10.3390/electronics14142876 - 18 Jul 2025
Abstract
Evaluating the classification accuracy of machine learning models typically involves multiple rounds of random training/test splits, model retraining, and performance averaging. However, this conventional approach is computationally expensive and time-consuming, especially for large datasets or complex models. To address this issue, we propose [...] Read more.
Evaluating the classification accuracy of machine learning models typically involves multiple rounds of random training/test splits, model retraining, and performance averaging. However, this conventional approach is computationally expensive and time-consuming, especially for large datasets or complex models. To address this issue, we propose an effective sampling approach that selects a single training/test split that closely approximates the results obtained from repeated random sampling. Our approach ensures that the sampled data closely reflects the classification performance of the original dataset. Our methods integrate advanced distribution distance metrics and feature weighting techniques tailored for numerical, categorical, and mixed-type datasets. The experimental results demonstrate that our method achieves over 95% agreement with multi-run average accuracy while reducing the overhead of computations by more than 90%. This approach offers a scalable, resource-efficient alternative for reliable model evaluation, particularly valuable in time-critical or resource-constrained applications. Full article
(This article belongs to the Special Issue Data Retrieval and Data Mining)
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27 pages, 3704 KiB  
Article
Explainable Machine Learning and Predictive Statistics for Sustainable Photovoltaic Power Prediction on Areal Meteorological Variables
by Sajjad Nematzadeh and Vedat Esen
Appl. Sci. 2025, 15(14), 8005; https://doi.org/10.3390/app15148005 - 18 Jul 2025
Abstract
Precisely predicting photovoltaic (PV) output is crucial for reliable grid integration; so far, most models rely on site-specific sensor data or treat large meteorological datasets as black boxes. This study proposes an explainable machine-learning framework that simultaneously ranks the most informative weather parameters [...] Read more.
Precisely predicting photovoltaic (PV) output is crucial for reliable grid integration; so far, most models rely on site-specific sensor data or treat large meteorological datasets as black boxes. This study proposes an explainable machine-learning framework that simultaneously ranks the most informative weather parameters and reveals their physical relevance to PV generation. Starting from 27 local and plant-level variables recorded at 15 min resolution for a 1 MW array in Çanakkale region, Türkiye (1 August 2022–3 August 2024), we apply a three-stage feature-selection pipeline: (i) variance filtering, (ii) hierarchical correlation clustering with Ward linkage, and (iii) a meta-heuristic optimizer that maximizes a neural-network R2 while penalizing poor or redundant inputs. The resulting subset, dominated by apparent temperature and diffuse, direct, global-tilted, and terrestrial irradiance, reduces dimensionality without significantly degrading accuracy. Feature importance is then quantified through two complementary aspects: (a) tree-based permutation scores extracted from a set of ensemble models and (b) information gain computed over random feature combinations. Both views converge on shortwave, direct, and global-tilted irradiance as the primary drivers of active power. Using only the selected features, the best model attains an average R2 ≅ 0.91 on unseen data. By utilizing transparent feature-reduction techniques and explainable importance metrics, the proposed approach delivers compact, more generalized, and reliable PV forecasts that generalize to sites lacking embedded sensor networks, and it provides actionable insights for plant siting, sensor prioritization, and grid-operation strategies. Full article
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21 pages, 9571 KiB  
Article
Performance Evaluation of Real-Time Image-Based Heat Release Rate Prediction Model Using Deep Learning and Image Processing Methods
by Joohyung Roh, Sehong Min and Minsuk Kong
Fire 2025, 8(7), 283; https://doi.org/10.3390/fire8070283 - 18 Jul 2025
Abstract
Heat release rate (HRR) is a key indicator for characterizing fire behavior, and it is conventionally measured under laboratory conditions. However, this measurement is limited in its widespread application to various fire conditions, due to its high cost, operational complexity, and lack of [...] Read more.
Heat release rate (HRR) is a key indicator for characterizing fire behavior, and it is conventionally measured under laboratory conditions. However, this measurement is limited in its widespread application to various fire conditions, due to its high cost, operational complexity, and lack of real-time predictive capability. Therefore, this study proposes an image-based HRR prediction model that uses deep learning and image processing techniques. The flame region in a fire video was segmented using the YOLO-YCbCr model, which integrates YCbCr color-space-based segmentation with YOLO object detection. For comparative analysis, the YOLO segmentation model was used. Furthermore, the fire diameter and flame height were determined from the spatial information of the segmented flame, and the HRR was predicted based on the correlation between flame size and HRR. The proposed models were applied to various experimental fire videos, and their prediction performances were quantitatively assessed. The results indicated that the proposed models accurately captured the HRR variations over time, and applying the average flame height calculation enhanced the prediction performance by reducing fluctuations in the predicted HRR. These findings demonstrate that the image-based HRR prediction model can be used to estimate real-time HRR values in diverse fire environments. Full article
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11 pages, 211 KiB  
Article
Splenic Torsion Following Blunt Abdominal Trauma
by Piotr Tomasz Arkuszewski, Agata Grochowska, Wiktoria Jachymczak and Karol Kamil Kłosiński
J. Clin. Med. 2025, 14(14), 5107; https://doi.org/10.3390/jcm14145107 - 18 Jul 2025
Abstract
Background/Objectives: Splenic torsion is a well-known and reported clinical problem. Splenic torsions after abdominal trauma represent a small group of cases that involve surgical management. They manifest primarily as abdominal pain, and the diagnosis is made based on imaging studies—ultrasound, CT, and [...] Read more.
Background/Objectives: Splenic torsion is a well-known and reported clinical problem. Splenic torsions after abdominal trauma represent a small group of cases that involve surgical management. They manifest primarily as abdominal pain, and the diagnosis is made based on imaging studies—ultrasound, CT, and MRI. Methods: This work aimed to analyze traumatic splenic torsions in terms of their clinical course, symptoms, timing, involvement of imaging techniques in the diagnosis, histopathological examination, and overall outcome. We searched databases using the desk research method under the keywords “splenic torsion”, “torsion”, and “spleen”, as well as in combination with “traumatic”, finding a total of eight cases, which we included in our analysis. Results: The eight cases were analyzed, comprising four females and four males, with an average age of 16.25 years (range 5–29 years). Traffic accidents were the most frequent cause of injury (five cases), while the circumstances were unclear in the remaining three. Immediate abdominal symptoms appeared in six patients. Splenic torsion was preoperatively diagnosed in five out of seven confirmed cases. A total of seven patients underwent laparotomy with splenectomy. In one case, laparoscopy converted to laparotomy with splenopexy preserved the spleen. Histopathology, performed in only two cases, confirmed splenic infarction in one patient; infarction status could not be determined in the remaining five due to missing data. Conclusions: Post-traumatic splenic torsions are a group of atypical injuries as the primary and immediate consequence of the trauma suffered is not anatomical–structural damage to the organ, such as a rupture. Mostly affecting young people, the cases described in the professional literature involve the main spleen, which was considered to be “wandering”, suggesting that this is a key predisposing factor for splenic torsion following blunt trauma and requiring diagnostic imaging for diagnosis. Full article
(This article belongs to the Special Issue Recent Advances in Therapy of Trauma and Surgical Critical Care)
20 pages, 359 KiB  
Article
Iterative Matrix Techniques Based on Averages
by María A. Navascués
Algorithms 2025, 18(7), 439; https://doi.org/10.3390/a18070439 - 17 Jul 2025
Abstract
Matrices have an important role in modern engineering problems like artificial intelligence, biomedicine, machine learning, etc. The present paper proposes new algorithms to solve linear problems involving finite matrices as well as operators in infinite dimensions. It is well known that the power [...] Read more.
Matrices have an important role in modern engineering problems like artificial intelligence, biomedicine, machine learning, etc. The present paper proposes new algorithms to solve linear problems involving finite matrices as well as operators in infinite dimensions. It is well known that the power method to find an eigenvalue and an eigenvector of a matrix requires the existence of a dominant eigenvalue. This article proposes an iterative method to find eigenvalues of matrices without a dominant eigenvalue. This algorithm is based on a procedure involving averages of the mapping and the independent variable. The second contribution is the computation of an eigenvector associated with a known eigenvalue of linear operators or matrices. Then, a novel numerical method for solving a linear system of equations is studied. The algorithm is especially suitable for cases where the iteration matrix has a norm equal to one or the standard iterative method based on fixed point approximation converges very slowly. These procedures are applied to the resolution of Fredholm integral equations of the first kind with an arbitrary kernel by means of orthogonal polynomials, and in a particular case where the kernel is separable. Regarding the latter case, this paper studies the properties of the associated Fredholm operator. Full article
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23 pages, 14080 KiB  
Article
Regional Ecological Environment Quality Prediction Based on Multi-Model Fusion
by Yiquan Song, Zhengwei Li and Baoquan Wei
Land 2025, 14(7), 1486; https://doi.org/10.3390/land14071486 - 17 Jul 2025
Abstract
Regional ecological environmental quality (EEQ) is a vital indicator for environmental management and supporting sustainable development. However, the absence of robust and accurate EEQ prediction models has hindered effective environmental strategies. This study proposes a novel approach to address this gap by integrating [...] Read more.
Regional ecological environmental quality (EEQ) is a vital indicator for environmental management and supporting sustainable development. However, the absence of robust and accurate EEQ prediction models has hindered effective environmental strategies. This study proposes a novel approach to address this gap by integrating the ecological index (EI) model with several predictive models, including autoregressive integrated moving average (ARIMA), convolutional neural network (CNN), long short-term memory (LSTM), and cellular automata (CA), to forecast regional EEQ. Initially, the spatiotemporal evolution of the input data used to calculate the EI score was analyzed. Subsequently, tailored prediction models were developed for each dataset. These models were sequentially trained and validated, and their outputs were integrated into the EI model to enhance the accuracy and coherence of the final EEQ predictions. The novelty of this methodology lies not only in integrating existing predictive models but also in employing an innovative fusion technique that significantly improves prediction accuracy. Despite data quality issues in the case study dataset led to higher prediction errors in certain regions, the overall results exhibit a high degree of accuracy. A comparison of long-term EI predictions with EI assessment results reveals that the R2 value for the EI score exceeds 0.96, and the kappa value surpasses 0.76 for the EI level, underscoring the robust performance of the integrated model in forecasting regional EEQ. This approach offers valuable insights into exploring regional EEQ trends and future challenges. Full article
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21 pages, 6787 KiB  
Article
Fast Calculation of Thermal-Fluid Coupled Transient Multi-Physics Field in Transformer Based on Extended Dynamic Mode Decomposition
by Yanming Cao, Kanghang He, Wenyuan Shangguan, Yuqi Wang and Chunjia Gao
Processes 2025, 13(7), 2282; https://doi.org/10.3390/pr13072282 - 17 Jul 2025
Abstract
With the development of digital power systems, the establishment of digital twin models for transformers is of great significance in enhancing power system stability. Consequently, greater demands are placed on the real-time performance and accuracy of thermal-fluid-coupled transient multi-physics field calculations for transformers. [...] Read more.
With the development of digital power systems, the establishment of digital twin models for transformers is of great significance in enhancing power system stability. Consequently, greater demands are placed on the real-time performance and accuracy of thermal-fluid-coupled transient multi-physics field calculations for transformers. However, traditional numerical methods, such as finite element or computational fluid dynamics techniques, often require days or even weeks to simulate full-scale high-fidelity transformer models containing millions of grid nodes. The high computational cost and long runtime make them impractical for real-time simulations in digital twin applications. To address this, this paper employs the dynamic mode decomposition (DMD) method in conjunction with Koopman operator theory to perform data-driven reduced-order modeling of the transformer’s thermal–fluid-coupled multi-physics field. A fast computational approach based on extended dynamic mode decomposition (EDMD) is proposed to enhance the modal decomposition capability of nonlinear systems and improve prediction accuracy. The results show that this method greatly improves computational efficiency while preserving accuracy in high-fidelity models with millions of grids. The errors in the thermal and flow field calculations remain below 3.06% and 3.01%, respectively. Furthermore, the computation time is reduced from hours to minutes, with the thermal field achieving a 97-fold speed-up and the flow field an 83-fold speed-up, yielding an average speed-up factor of 90. This enables fast computation of the transformer’s thermal–fluid-coupled field and provides effective support for the application of digital twin technology in multi-physics field simulations of power equipment. Full article
(This article belongs to the Section Chemical Processes and Systems)
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27 pages, 8154 KiB  
Article
Investigation into the Efficient Cooperative Planning Approach for Dual-Arm Picking Sequences of Dwarf, High-Density Safflowers
by Zhenguo Zhang, Peng Xu, Binbin Xie, Yunze Wang, Ruimeng Shi, Junye Li, Wenjie Cao, Wenqiang Chu and Chao Zeng
Sensors 2025, 25(14), 4459; https://doi.org/10.3390/s25144459 - 17 Jul 2025
Abstract
Path planning for picking safflowers is a key component in ensuring the efficient operation of robotic safflower-picking systems. However, existing single-arm picking devices have become a bottleneck due to their limited operating range, and a breakthrough in multi-arm cooperative picking is urgently needed. [...] Read more.
Path planning for picking safflowers is a key component in ensuring the efficient operation of robotic safflower-picking systems. However, existing single-arm picking devices have become a bottleneck due to their limited operating range, and a breakthrough in multi-arm cooperative picking is urgently needed. To address the issue of inadequate adaptability in current path planning strategies for dual-arm systems, this paper proposes a novel path planning method for dual-arm picking (LTSACO). The technique centers on a dynamic-weight heuristic strategy and achieves optimization through the following steps: first, the K-means clustering algorithm divides the target area; second, the heuristic mechanism of the Ant Colony Optimization (ACO) algorithm is improved by dynamically adjusting the weight factor of the state transition probability, thereby enhancing the diversity of path selection; third, a 2-OPT local search strategy eliminates path crossings through neighborhood search; finally, a cubic Bézier curve heuristically smooths and optimizes the picking trajectory, ensuring the continuity of the trajectory’s curvature. Experimental results show that the length of the parallelogram trajectory, after smoothing with the Bézier curve, is reduced by 20.52% compared to the gantry trajectory. In terms of average picking time, the LTSACO algorithm reduces the time by 2.00%, 2.60%, and 5.60% compared to DCACO, IACO, and the traditional ACO algorithm, respectively. In conclusion, the LTSACO algorithm demonstrates high efficiency and strong robustness, providing an effective optimization solution for multi-arm cooperative picking and significantly contributing to the advancement of multi-arm robotic picking systems. Full article
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23 pages, 6440 KiB  
Article
A Gravity Data Denoising Method Based on Multi-Scale Attention Mechanism and Physical Constraints Using U-Net
by Bing Liu, Houpu Li, Shaofeng Bian, Chaoliang Zhang, Bing Ji and Yujie Zhang
Appl. Sci. 2025, 15(14), 7956; https://doi.org/10.3390/app15147956 - 17 Jul 2025
Abstract
Gravity and gravity gradient data serve as fundamental inputs for geophysical resource exploration and geological structure analysis. However, traditional denoising methods—including wavelet transforms, moving averages, and low-pass filtering—exhibit signal loss and limited adaptability under complex, non-stationary noise conditions. To address these challenges, this [...] Read more.
Gravity and gravity gradient data serve as fundamental inputs for geophysical resource exploration and geological structure analysis. However, traditional denoising methods—including wavelet transforms, moving averages, and low-pass filtering—exhibit signal loss and limited adaptability under complex, non-stationary noise conditions. To address these challenges, this study proposes an improved U-Net deep learning framework that integrates multi-scale feature extraction and attention mechanisms. Furthermore, a Laplace consistency constraint is introduced into the loss function to enhance denoising performance and physical interpretability. Notably, the datasets used in this study are generated by the authors, involving simulations of subsurface prism distributions with realistic density perturbations (±20% of typical rock densities) and the addition of controlled Gaussian noise (5%, 10%, 15%, and 30%) to simulate field-like conditions, ensuring the diversity and physical relevance of training samples. Experimental validation on these synthetic datasets and real field datasets demonstrates the superiority of the proposed method over conventional techniques. For noise levels of 5%, 10%, 15%, and 30% in test sets, the improved U-Net achieves Peak Signal-to-Noise Ratios (PSNR) of 59.13 dB, 52.03 dB, 48.62 dB, and 48.81 dB, respectively, outperforming wavelet transforms, moving averages, and low-pass filtering by 10–30 dB. In multi-component gravity gradient denoising, our method excels in detail preservation and noise suppression, improving Structural Similarity Index (SSIM) by 15–25%. Field data tests further confirm enhanced identification of key geological anomalies and overall data quality improvement. In summary, the improved U-Net not only delivers quantitative advancements in gravity data denoising but also provides a novel approach for high-precision geophysical data preprocessing. Full article
(This article belongs to the Special Issue Applications of Machine Learning in Earth Sciences—2nd Edition)
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22 pages, 1718 KiB  
Review
A Review on Risk and Reliability Analysis in Photovoltaic Power Generation
by Ahmad Zaki Abdul Karim, Mohamad Shaiful Osman and Mohd. Khairil Rahmat
Energies 2025, 18(14), 3790; https://doi.org/10.3390/en18143790 - 17 Jul 2025
Abstract
Precise evaluation of risk and reliability is crucial for decision making and predicting the outcome of investment in a photovoltaic power system (PVPS) due to its intermittent source. This paper explores different methodologies for risk evaluation and reliability assessment, which can be categorized [...] Read more.
Precise evaluation of risk and reliability is crucial for decision making and predicting the outcome of investment in a photovoltaic power system (PVPS) due to its intermittent source. This paper explores different methodologies for risk evaluation and reliability assessment, which can be categorized into qualitative, quantitative, and hybrid qualitative and quantitative (HQQ) approaches. Qualitative methods include failure mode analysis, graphical analysis, and hazard analysis, while quantitative methods include analytical methods, stochastic methods, Bayes’ theorem, reliability optimization, multi-criteria analysis, and data utilization. HQQ methodology combines table-based and visual analysis methods. Currently, reliability assessment techniques such as mean time between failures (MTBF), system average interruption frequency index (SAIFI), and system average interruption duration index (SAIDI) are commonly used to predict PVPS performance. However, alternative methods such as economical metrics like the levelized cost of energy (LCOE) and net present value (NPV) can also be used. Therefore, a risk and reliability approach should be applied together to improve the accuracy of predicting significant aspects in the photovoltaic industry. Full article
(This article belongs to the Section B: Energy and Environment)
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16 pages, 1934 KiB  
Systematic Review
The Changes of Kahweol and Cafestol of Arabica Coffee from Bean to Consumption: A Systematic Literature Review
by A. Ita Juwita, Didah Nur Faridah, Dian Herawati, Nuri Andarwulan, Renata Kazimierczak and Dominika Średnicka-Tober
Beverages 2025, 11(4), 105; https://doi.org/10.3390/beverages11040105 - 17 Jul 2025
Abstract
The main diterpenes found in coffee, kahweol and cafestol, possess anti-inflammatory, anti-diabetic, and anticancer properties but are also reported to cause hypercholesterolemic effects. Their concentrations are known to be variable in coffee. This review aimed to discuss the concentrations of kahweol and cafestol [...] Read more.
The main diterpenes found in coffee, kahweol and cafestol, possess anti-inflammatory, anti-diabetic, and anticancer properties but are also reported to cause hypercholesterolemic effects. Their concentrations are known to be variable in coffee. This review aimed to discuss the concentrations of kahweol and cafestol from green coffee beans to brewed coffee. The results showed that the average concentrations of kahweol and cafestol in Arabica green beans were higher than in roasted and brewed coffee. The decrease in kahweol from green beans to roasted beans was 14.83%. In brewed coffee, kahweol was reduced by 90.26% and cafestol by 88.28%, compared to green beans. The changes in kahweol and cafestol levels were found to be influenced by various factors, including roasting methods and brewing techniques. The ratio of kahweol to cafestol in Arabica green beans was 1.7; in green coffee oil and roasted coffee oil, 1.2; in roasted beans, 1.3; and in brewed coffee, 1.1. In addition to their health-related functional properties, kahweol and cafestol concentrations and their ratio are suggested to be relevant markers in distinguishing between coffee species at various processing stages. Full article
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22 pages, 4837 KiB  
Article
Leveraging Historical Process Data for Recombinant P. pastoris Fermentation Hybrid Deep Modeling and Model Predictive Control Development
by Emils Bolmanis, Vytautas Galvanauskas, Oskars Grigs, Juris Vanags and Andris Kazaks
Fermentation 2025, 11(7), 411; https://doi.org/10.3390/fermentation11070411 - 17 Jul 2025
Abstract
Hybrid modeling techniques are increasingly important for improving predictive accuracy and control in biomanufacturing, particularly in data-limited conditions. This study develops and experimentally validates a hybrid deep learning model predictive control (MPC) framework for recombinant P. pastoris fed-batch fermentations. Bayesian optimization and grid [...] Read more.
Hybrid modeling techniques are increasingly important for improving predictive accuracy and control in biomanufacturing, particularly in data-limited conditions. This study develops and experimentally validates a hybrid deep learning model predictive control (MPC) framework for recombinant P. pastoris fed-batch fermentations. Bayesian optimization and grid search techniques were employed to identify the best-performing hybrid model architecture: an LSTM layer with 2 hidden units followed by a fully connected layer with 8 nodes and ReLU activation. This design balanced accuracy (NRMSE 4.93%) and computational efficiency (AICc 998). This architecture was adapted to a new, smaller dataset of bacteriophage Qβ coat protein production using transfer learning, yielding strong predictive performance with low validation (3.53%) and test (5.61%) losses. Finally, the hybrid model was integrated into a novel MPC system and experimentally validated, demonstrating robust real-time substrate feed control in a way that allows it to maintain specific target growth rates. The system achieved predictive accuracies of 6.51% for biomass and 14.65% for product estimation, with an average tracking error of 10.64%. In summary, this work establishes a robust, adaptable, and efficient hybrid modeling framework for MPC in P. pastoris bioprocesses. By integrating automated architecture searching, transfer learning, and MPC, the approach offers a practical and generalizable solution for real-time control and supports scalable digital twin deployment in industrial biotechnology. Full article
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