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20 pages, 351 KB  
Article
The Role of Ritual Prayer (Ṣalāh) in Self-Purification and Identity Formation: An Islamic Educational Perspective
by Adeeb Obaid Alsuhaymi and Fouad Ahmed Atallah
Religions 2025, 16(11), 1347; https://doi.org/10.3390/rel16111347 (registering DOI) - 25 Oct 2025
Abstract
Ritual prayer (ṣalāh) is one of the most central and enduring practices in Islam, widely recognized for its spiritual significance. However, its educational and formative role in shaping the Muslim’s inner self and moral identity remains insufficiently explored in contemporary scholarship. This paper [...] Read more.
Ritual prayer (ṣalāh) is one of the most central and enduring practices in Islam, widely recognized for its spiritual significance. However, its educational and formative role in shaping the Muslim’s inner self and moral identity remains insufficiently explored in contemporary scholarship. This paper aims to examine ritual prayer as a core pedagogical tool within Islamic education, focusing on its transformative power in the processes of self-purification (tazkiyah) and identity formation. The study seeks to analyze the ethical and psychological dimensions of ṣalāh, drawing on classical Islamic sources, as well as integrating insights from contemporary critical philosophy—particularly Byung-Chul Han’s Vita Contemplativa—and Islamic virtue ethics, including perspectives such as those advanced by Elizabeth Bucar. Through this framework, the paper explores how prayer shapes inner dispositions like humility, mindfulness, sincerity, patience, and submission, reinforcing both spiritual awareness and communal belonging. Employing a descriptive-analytical methodology, the study engages Qur’anic verses, prophetic traditions, and traditional pedagogical literature to investigate how ṣalāh functions as a lived and repeated experience that cultivates the soul and molds ethical behavior. The discussion highlights how regular performance of prayer integrates belief with action and contributes to the formation of a reflective and morally grounded Muslim identity. This paper contributes to the field of Islamic Practical Theology by demonstrating how ritual prayer operates as a dynamic and holistic model for moral and spiritual development. It provides educators and scholars with a theoretical and applied vision for incorporating ṣalāh-based character education into Islamic curricula. Future research may explore how prayer interacts with modern lifestyles, digital spiritual practices, and intergenerational transmission of religious identity in diverse contexts. Full article
(This article belongs to the Special Issue Islamic Practical Theology)
32 pages, 6328 KB  
Article
A Combined Experimental, Theoretical, and Simulation Approach to the Effects of GNPs and MWCNTs on Joule Heating Behavior of 3D Printed PVDF Nanocomposites
by Giovanni Spinelli, Rosella Guarini, Rumiana Kotsilkova, Evgeni Ivanov and Vladimir Georgiev
Polymers 2025, 17(21), 2835; https://doi.org/10.3390/polym17212835 (registering DOI) - 24 Oct 2025
Abstract
The thermal behavior of 3D-printed polyvinylidene fluoride (PVDF)-based composites enhanced with carbon nanotubes (CNTs), graphene nanoplatelets (GNPs), and their hybrid formulations was investigated under Joule heating at applied voltages of 2, 3, and 4 V. The influence of filler type and weight fraction [...] Read more.
The thermal behavior of 3D-printed polyvinylidene fluoride (PVDF)-based composites enhanced with carbon nanotubes (CNTs), graphene nanoplatelets (GNPs), and their hybrid formulations was investigated under Joule heating at applied voltages of 2, 3, and 4 V. The influence of filler type and weight fraction on both electrical and thermal conductivity was systematically assessed using a Design of Experiments (DoE) approach. Response Surface Methodology (RSM) was employed to derive an analytical relationship linking conductivity values to filler loading, revealing clear trends and interaction effects. Among all tested formulations, the composite containing 6 wt% of GNPs exhibited the highest performance in terms of thermal response and electrical conductivity, reaching a steady-state temperature of 88.1 °C under an applied voltage of just 4 V. This optimal formulation was further analyzed through multiphysics simulations, validated against experimental data and theoretical predictions, to evaluate its effectiveness for potential practical applications—particularly in de-icing systems leveraging Joule heating. The integrated experimental–theoretical–numerical workflow proposed herein offers a robust strategy for guiding the development and optimization of next-generation polymer nanocomposites for thermal management technologies. Full article
(This article belongs to the Section Polymer Composites and Nanocomposites)
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23 pages, 1745 KB  
Article
Multi-Dimensional Risks and Eco-Environmental Responses of Check Dam Systems: Evidence from a Typical Watershed in China’s Loess Plateau
by Yujie Yang, Shengdong Cheng, Penglei Hang, Zhanbin Li, Heng Wu, Ganggang Ke, Xingyue Guo and Yunzhe Zhen
Sustainability 2025, 17(21), 9477; https://doi.org/10.3390/su17219477 (registering DOI) - 24 Oct 2025
Abstract
Deteriorating check dams pose significant threats to human safety and property, while impeding eco-environmental restoration in soil–water conservation systems in vulnerable watersheds like the Jiuyuangou Basin on China’s Loess Plateau. This study aimed to develop a comprehensive risk assessment framework for the check [...] Read more.
Deteriorating check dams pose significant threats to human safety and property, while impeding eco-environmental restoration in soil–water conservation systems in vulnerable watersheds like the Jiuyuangou Basin on China’s Loess Plateau. This study aimed to develop a comprehensive risk assessment framework for the check dam system in the Jiuyuangou Basin, China, to mitigate its threats to safety and eco-environmental restoration. A multi-index and multilevel risk evaluation system was established for check dam systems in the Jiuyuangou Basin, utilizing data gathering, hydrological statistics, numerical computation, and various methodologies. The index weights were determined via the fuzzy analytic hierarchy process with an integrated modeling framework for key parameters. Finally, the risk level of the check dam system in the Jiuyuangou Basin was assessed based on the comprehensive score. The results show that (1) nearly half of the check dams are at mild risk, approximately 25% are at moderate risk, and a few are basically safe. (2) Among various types of risk, the distribution of engineering risk is relatively uniform, environmental risk is generally high, loss risk is relatively concentrated, and management risk is particularly prominent. This research provides a scientific foundation for optimizing check dam governance, enhancing sediment control, and strengthening ecological service functions in vulnerable watersheds. Full article
(This article belongs to the Special Issue Ecological Water Engineering and Ecological Environment Restoration)
51 pages, 6941 KB  
Review
From Fundamentals of Laser-Induced Breakdown Spectroscopy to Recent Advancements in Cancer Detection and Calcified Tissues Analysis: An Overview (2015–2025)
by Muhammad Mustafa Dastageer, Khurram Siraj, Johannes David Pedarnig, Dacheng Zhang, Muhammad Qasim, Muhammad Shahzad Abdul Rahim, Saba Mushtaq, Qaneeta Younas and Bareera Hussain
Molecules 2025, 30(21), 4176; https://doi.org/10.3390/molecules30214176 (registering DOI) - 24 Oct 2025
Abstract
Laser-induced breakdown spectroscopy (LIBS) is a promising elemental analysis technique that has rapidly evolved in numerous fields, including biomedical research and medical sciences, over the last two decades. In combination with other methods, it has the potential to examine complex biological structures and [...] Read more.
Laser-induced breakdown spectroscopy (LIBS) is a promising elemental analysis technique that has rapidly evolved in numerous fields, including biomedical research and medical sciences, over the last two decades. In combination with other methods, it has the potential to examine complex biological structures and their species distributions. The present work first develops the basic understanding of LIBS and then reviews its evolution in oncological diagnosis and calcified tissue analysis from medical perspectives over the last 11 years. LIBS can potentially improve early cancer detection and monitor treatment outcomes, ultimately enhancing patient care and diagnosis. It has effectively differentiated between malignant and normal tissues and also classifies cancer stages and types based on disease severity. Its applications for categorising and identifying calcified tissues are attractive for inspecting minerals, while soft tissue is more challenging, given the potential for significant matrix effects. This review article deals with the following aspects of LIBS and its application: (i) the fundamentals of this analytical measurement method, (ii) the matrix effect and its influence on the LIBS analyses of various biological tissues, (iii) the role of signal enhancement methodologies and artificial intelligence models to advance the method for analyses of biological sample materials, and (iv) applications of LIBS in cancer and calcified tissues investigations. This article also addresses challenges and opportunities encountered in these applications and discusses prospects, providing a comprehensive overview of the current state and potential advancement in LIBS technology. Full article
(This article belongs to the Special Issue Review Papers in Analytical Chemistry, 2nd Edition)
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26 pages, 3483 KB  
Review
UHPLC-MS/MS for Antipsychotic Drug Monitoring: A Systematic Review of Clinical and Analytical Performance
by Ciprian-Ionuț Băcilă, Bianca-Maria Macavei, Monica Cornea, Bogdan Ioan Vintilă, Andrei Lomnășan, Claudia Elena Anghel, Andreea Maria Grama, Cristina Elena Dobre, Claudia Marina Ichim and Gabriela Cioca
J. Clin. Med. 2025, 14(21), 7544; https://doi.org/10.3390/jcm14217544 (registering DOI) - 24 Oct 2025
Abstract
Background/Objectives: Therapeutic drug monitoring (TDM) of antipsychotic medications plays an important role in optimizing treatment efficacy, reducing adverse effects, and supporting adherence. While Ultra-High Performance Liquid Chromatography–Tandem Mass Spectrometry (UHPLC–MS/MS) has long been the gold standard for antipsychotic quantification, recent advances in [...] Read more.
Background/Objectives: Therapeutic drug monitoring (TDM) of antipsychotic medications plays an important role in optimizing treatment efficacy, reducing adverse effects, and supporting adherence. While Ultra-High Performance Liquid Chromatography–Tandem Mass Spectrometry (UHPLC–MS/MS) has long been the gold standard for antipsychotic quantification, recent advances in automated platforms and microsampling raise questions about its current clinical practicality. This systematic review evaluated the clinical applicability and analytical performance of UHPLC-based methods for monitoring antipsychotic drugs, focusing on precision, recovery, matrix effects, and suitability across various biological matrices. Methods: A systematic search of PubMed, Scopus, and Web of Science was conducted for studies published between 2013 and 2024 involving UHPLC-based quantification of antipsychotics in clinical samples from adult patients. Data on analytical parameters, sample matrices, and study characteristics were extracted. A custom quality checklist was used to assess methodological rigor. In addition to qualitative synthesis, non-traditional quantitative approaches were applied, including descriptive aggregation of recovery, matrix effects, and precision across studies, as well as correlation analyses to explore relationships among performance parameters. Results: Twelve studies were included, spanning a range of typical and atypical antipsychotics and metabolites. Plasma and serum demonstrated the highest analytical reliability (recovery >90%, minimal matrix effects), while dried blood spots (DBSs), whole blood, and oral fluid showed greater variability. Clinically, UHPLC–MS/MS enabled more accurate dose adjustments and identification of non-adherence, outperforming immunoassays in sensitivity, specificity, and metabolite detection. Microsampling methods showed promise for outpatient and decentralized care but require further clinical validation. Conclusions: UHPLC–MS/MS remains the most robust and reliable method for TDM of antipsychotics, especially when quantification of active metabolites is required. While logistical barriers remain, technological advances may enhance feasibility and support broader integration into routine psychiatric care. Full article
(This article belongs to the Special Issue Advancements and Future Directions in Clinical Psychosis)
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33 pages, 2850 KB  
Review
Network Traffic Analysis Based on Graph Neural Networks: A Scoping Review
by Ruonan Wang, Jinjing Zhao, Hongzheng Zhang, Liqiang He, Hu Li and Minhuan Huang
Big Data Cogn. Comput. 2025, 9(11), 270; https://doi.org/10.3390/bdcc9110270 (registering DOI) - 24 Oct 2025
Abstract
Network traffic analysis is crucial for understanding network behavior and identifying underlying applications, protocols, and service groups. The increasing complexity of network environments, driven by the evolution of the Internet, poses significant challenges to traditional analytical approaches. Graph Neural Networks (GNNs) have recently [...] Read more.
Network traffic analysis is crucial for understanding network behavior and identifying underlying applications, protocols, and service groups. The increasing complexity of network environments, driven by the evolution of the Internet, poses significant challenges to traditional analytical approaches. Graph Neural Networks (GNNs) have recently garnered considerable attention in network traffic analysis due to their ability to model complex relationships within network flows and between communicating entities. This scoping review systematically surveys major academic databases, employing predefined eligibility criteria to identify and synthesize key research in the field, following the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) methodology. We present a comprehensive overview of a generalized architecture for GNN-based traffic analysis and categorize recent methods into three primary types: node prediction, edge prediction, and graph prediction. We discuss challenges in network traffic analysis, summarize solutions from various methods, and provide practical recommendations for model selection. This review also compiles publicly available datasets and open-source code, serving as valuable resources for further research. Finally, we outline future research directions to advance this field. This work offers an updated understanding of GNN applications in network traffic analysis and provides practical guidance for researchers and practitioners. Full article
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28 pages, 770 KB  
Review
Leveraging Artificial Intelligence and Modulation of Oxidative Stressors to Enhance Healthspan and Radical Longevity
by Donald D. Haines, Stephen Christopher Rose, Fred M. Cowan, Fadia F. Mahmoud, Albert A. Rizvanov and Arpad Tosaki
Biomolecules 2025, 15(11), 1501; https://doi.org/10.3390/biom15111501 (registering DOI) - 24 Oct 2025
Abstract
This review explores the transformative potentials of artificial intelligence (AI) in promoting healthspan and longevity. Healthspan focuses on enhancing quality of life free from chronic conditions, while longevity defines current lifespan limits within a particular species and encompasses biological aging at multiple levels. [...] Read more.
This review explores the transformative potentials of artificial intelligence (AI) in promoting healthspan and longevity. Healthspan focuses on enhancing quality of life free from chronic conditions, while longevity defines current lifespan limits within a particular species and encompasses biological aging at multiple levels. AI methodologies—including machine learning, deep learning, natural language processing, robotics, and data analytics—offer unprecedented tools to analyze complex biological data, accelerate biomarker discovery, optimize therapeutic interventions, and personalize medicine. Notably, AI has facilitated breakthroughs in identifying accurate biomarkers of biological age, developing precision medicine approaches, accelerating drug discovery, and enhancing genomic editing technologies such as CRISPR. Further, AI-based analysis of endogenous cytoprotection, especially the activity of molecules such as heme oxygenase, with particular application to hemolytic diseases. AI-driven robotics and automated monitoring systems significantly improve elderly care, lifestyle interventions, and clinical trials, demonstrating considerable potential to extend both healthspan and lifespan. However, the integration of AI into longevity research poses ethical and societal challenges, including concerns over privacy, equitable access, and broader implications of extended human lifespans. Strategic interdisciplinary collaboration, transparent AI methodologies, standardized data frameworks, and equitable policy approaches are essential to responsibly harness AI’s full potential in transforming longevity science and improving human health. Full article
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22 pages, 964 KB  
Systematic Review
Using Data Analytics in Financial Statement Fraud Detection and Prevention: A Systematic Review of Methods, Challenges, and Future Directions
by Michail Gkegkas, Dimitrios Kydros and Michail Pazarskis
J. Risk Financial Manag. 2025, 18(11), 598; https://doi.org/10.3390/jrfm18110598 - 24 Oct 2025
Abstract
Reliable financial reporting is critical for maintaining market confidence and guiding stakeholders’ decision-making, yet traditional audit methods often fail to detect sophisticated fraud schemes that are hidden within large volumes of transactional data. This systematic literature review synthesizes 43 empirical and theoretical studies [...] Read more.
Reliable financial reporting is critical for maintaining market confidence and guiding stakeholders’ decision-making, yet traditional audit methods often fail to detect sophisticated fraud schemes that are hidden within large volumes of transactional data. This systematic literature review synthesizes 43 empirical and theoretical studies published between 2010 and 2024 that utilize data analytics techniques for the prevention and detection of fraud in financial statements. Following the PRISMA guidelines, we conducted a four-phase review—identification, screening, eligibility assessment, and inclusion—to ensure transparency and reproducibility. Our analysis categorizes techniques into supervised machine learning classifiers (e.g., decision trees and neural networks), statistical anomaly detection methods, network-based analyses, and real-time monitoring frameworks. We evaluate each approach’s comparative effectiveness, highlight persistent challenges such as data imbalance, model interpretability, and governance constraints, and also trace evolving methodological trends over time. The review reveals that integrating predictive analytics and continuous monitoring into accounting information systems can transform audits from reactive investigations into proactive fraud prevention mechanisms. We conclude by proposing a future research agenda focusing on developing explainable AI models for audit applications, establishing robust data governance frameworks to support automated monitoring, and conducting longitudinal field studies to assess the real-world impact of analytics-driven controls. Full article
(This article belongs to the Section Applied Economics and Finance)
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32 pages, 2575 KB  
Review
Industrial Site Selection: Methodologies, Advances and Challenges
by Dongbo Wang, Yubo Zhu, Xidao Mao, Jianyi Wang and Xiaohui Ji
Appl. Sci. 2025, 15(21), 11379; https://doi.org/10.3390/app152111379 (registering DOI) - 23 Oct 2025
Abstract
Industrial site selection holds strategic importance in the layout of industrial facilities. Scientific decision-making in site selection not only enhances the economic and technical feasibility of a project but also lays the foundation for sustainable development. However, industrial site selection is considered an [...] Read more.
Industrial site selection holds strategic importance in the layout of industrial facilities. Scientific decision-making in site selection not only enhances the economic and technical feasibility of a project but also lays the foundation for sustainable development. However, industrial site selection is considered an NP-hard problem. The criteria used to evaluate site suitability, the methods proven effective under different conditions, big data sources introduced, and the key data gaps, methodological limitations, and research priorities to improve decision quality are important for researchers and engineers. Based on the Web of Science (WOS) core collection as the data source, this paper retrieved the literature related to the themes of “industrial site selection” and “facility location decision making”, and selected 149 highly relevant papers. It systematically categorizes three mainstream site selection methods: operations research-based methods; the application of geographic information systems in site selection; and the application of artificial intelligence in site selection. On this basis, this paper provides a systematic review of the overall industrial site selection process and methodologies, aiming to offer references for subsequent site selection analysis research and practical site selection work. An “MCDM–GIS–AI” technology convergence roadmap is also proposed for industrial site selection to identify remaining research gaps and offer a set of “good-practice guidelines” to inform both practical applications and future analytical studies. Full article
(This article belongs to the Special Issue Applications of Big Data and Artificial Intelligence in Geoscience)
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41 pages, 3705 KB  
Article
An HACCP-Inspired Post-Evaluation Framework for Highway Preventive Maintenance: Methodology and Case Application
by Naren Fang, Chen Wang and Huanyu Chang
Appl. Sci. 2025, 15(21), 11377; https://doi.org/10.3390/app152111377 - 23 Oct 2025
Abstract
With the increasing age and traffic load of highway networks in China, preventive maintenance has become a critical strategy for extending pavement service life and improving infrastructure sustainability. However, the lack of standardized post-evaluation systems has hindered the scientific assessment of maintenance effectiveness. [...] Read more.
With the increasing age and traffic load of highway networks in China, preventive maintenance has become a critical strategy for extending pavement service life and improving infrastructure sustainability. However, the lack of standardized post-evaluation systems has hindered the scientific assessment of maintenance effectiveness. This study proposes a systematic post-evaluation framework for highway preventive maintenance projects based on the Hazard Analysis and Critical Control Points (HACCP)-Inspired methodology (Applying Principles of Hazard Analysis and CCP Identification). Adopting a full life-cycle perspective, the framework identifies critical control points (CCPs) across pre-, mid-, and post-implementation phases, targeting six key dimensions: ecological and environmental hazards, resource utilization hazard, engineering safety risks, engineering quality risks, socioeconomic benefit hazards, and social living environment hazards. A multi-level evaluation indicator system is constructed using hierarchical clustering and weighted through the Analytic Hierarchy Process (AHP). The framework is applied to a preventive maintenance project on the Jinghuan Expressway in Tianjin, China, demonstrating strong practical applicability. The final evaluation score of 84.1 out of 100 confirms the technical adequacy of the project while revealing areas for improvement in clean energy adoption and substructure monitoring. This framework provides a robust basis for standardizing post-evaluation practices and promoting sustainable highway maintenance management. Full article
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32 pages, 3974 KB  
Article
An Integrated Approach to the Development and Implementation of New Technological Solutions
by Dariusz Plinta and Katarzyna Radwan
Sustainability 2025, 17(21), 9434; https://doi.org/10.3390/su17219434 - 23 Oct 2025
Abstract
Dynamic technological changes and the variability of market requirements pose significant challenges for modern manufacturing companies in the effective development and implementation of new technological solutions. The aim of the research was to develop an integrated approach covering all key stages of implementation—from [...] Read more.
Dynamic technological changes and the variability of market requirements pose significant challenges for modern manufacturing companies in the effective development and implementation of new technological solutions. The aim of the research was to develop an integrated approach covering all key stages of implementation—from formulating technological solutions, through selecting and evaluating variants, to preparing and managing production processes—under the conditions of a medium-sized manufacturing company specializing in the batch production of steel constructions. The analysis was based on an interdisciplinary approach, combining methods of creative design of new technological solutions, including Blue Ocean Strategy, value proposition design, and QFD methodology, with analytical approaches that include multi-criteria evaluation of solution variants, technical preparation of production, as well as the organization and management of production processes in modified organizational conditions. This approach enabled a comprehensive assessment of the developed solutions, taking into account both their operational potential and practical feasibility in realistic implementation conditions, through the use of case studies and simulations to validate the results. The results of the research indicate that integrating methods for creating new solutions with analytical assessment and simulation tools leads to a more precise and data-driven approach to process design, enabling better decision-making based on thorough analysis and predictive modeling. Furthermore, this approach allows for a significant reduction in the risk of implementation failure through early identification of potential problems. The conclusion of the study confirms that a comprehensive and interdisciplinary approach to the implementation of new technologies ensures better alignment with customer demands, reduces production downtime, and enhances product optimization and resource utilization, which are critical factors in building a sustainable competitive advantage for manufacturing companies. The proposed approach enables more deliberate design and organization of manufacturing processes, supporting their flexible adaptation to changing market and technological conditions. Full article
(This article belongs to the Special Issue Innovative Technologies for Sustainable Industrial Systems)
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17 pages, 639 KB  
Article
A Multi-Criteria AHP-Based Framework for Sustainable Municipal Waste Collection
by Mattia Cottes and Patrizia Simeoni
Sustainability 2025, 17(21), 9430; https://doi.org/10.3390/su17219430 - 23 Oct 2025
Abstract
The management of waste has become increasingly complex due to the growing volume and diversity of waste generated by modern societies. Effective collection systems are essential for mitigating environmental impacts and promoting sustainability. However, the increasing complexity of waste management requires a comprehensive [...] Read more.
The management of waste has become increasingly complex due to the growing volume and diversity of waste generated by modern societies. Effective collection systems are essential for mitigating environmental impacts and promoting sustainability. However, the increasing complexity of waste management requires a comprehensive approach that considers multiple criteria in order to evaluate the performance of these systems. This study evaluates the environmental performance of waste collection systems by comparing various methods using the Analytic Hierarchy Process (AHP). The research involves identifying key performance indicators (KPIs) that could be relevant for all the stakeholders involved and important for environmental sustainability. These KPIs are then used as criteria for the AHP model, allowing for a detailed comparison of each collection method. Data is collected from a case study in the Friuli-Venezia Giulia region in Italy. The preliminary results indicate significant variations in environmental performance and user fruitfulness across different collection methods. Door-to-door collection was found to be the preferred methodology with an absolute weight of 0.527. The AHP framework proves to be a robust tool for integrating diverse criteria and stakeholder preferences, facilitating informed decision-making in waste management. Moreover, it underscores the importance of adopting a holistic approach to evaluate and improve recycling systems. By leveraging AHP, policymakers and waste management professionals can identify optimal strategies that align with environmental sustainability goals. Full article
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23 pages, 1286 KB  
Review
Microplastics in Airborne Particulate Matter: A Comprehensive Review of Separation Techniques, In Vitro Toxicity and Health Impacts
by Dominika Uchmanowicz, Katarzyna Styszko, Xijuan Chen, Giulia Terribile, Rakshit Jakhar, Giulio Sancini and Justyna Pyssa
Int. J. Mol. Sci. 2025, 26(21), 10332; https://doi.org/10.3390/ijms262110332 - 23 Oct 2025
Abstract
Microplastics (MPs) are emerging airborne pollutants that can migrate through various environmental pathways, with air representing one of the most critical exposure routes. Their occurrence within suspended particulate matter (PM)—a major atmospheric pollutant associated with respiratory, cardiovascular, and neurological diseases—further amplifies the risks [...] Read more.
Microplastics (MPs) are emerging airborne pollutants that can migrate through various environmental pathways, with air representing one of the most critical exposure routes. Their occurrence within suspended particulate matter (PM)—a major atmospheric pollutant associated with respiratory, cardiovascular, and neurological diseases—further amplifies the risks posed by air pollution. The main sources of airborne MPs include tire and road wear, degradation of larger plastic debris, and wind-driven resuspension from soil and landfills. This review provides a comprehensive synthesis of current knowledge on airborne MPs, integrating methodological and toxicological perspectives. It summarizes sampling and separation procedures (filtration, chemical digestion, density separation) and analytical techniques for qualitative and quantitative identification. Particular emphasis is placed on the toxicological implications of MPs, including oxidative stress, inflammatory responses, and potential carcinogenicity, as revealed by in vitro and mechanistic studies. In light of the absence of standardized methodologies, this work highlights the urgent need for harmonized protocols linking environmental monitoring with biological toxicity assessment. By combining information on analytical workflows and cellular responses, this review serves as a key reference for developing environmentally relevant experimental designs and evaluating health risks associated with airborne microplastics. It therefore bridges the gap between environmental analysis and toxicological research, outlining future priorities for methodological standardization and risk assessment. Full article
(This article belongs to the Special Issue Molecular Research on Micropollutants in Various Enviroments)
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29 pages, 619 KB  
Review
Flavonoids as Markers in Herbal Medicine Quality Control: Current Trends and Analytical Perspective
by Julia Morais Fernandes, Charlotte Silvestre, Silvana M. Zucolotto, Julien Antih, Fabrice Vaillant, Aude Echallier and Patrick Poucheret
Separations 2025, 12(11), 289; https://doi.org/10.3390/separations12110289 - 23 Oct 2025
Abstract
Flavonoids, a ubiquitous class of plant secondary metabolites, are increasingly pivotal as chemical markers for ensuring the quality, safety, and efficacy of herbal medicines (HMs). Their broad distribution, biological activities, and detectability make them ideal for this role. This comprehensive review critically examines [...] Read more.
Flavonoids, a ubiquitous class of plant secondary metabolites, are increasingly pivotal as chemical markers for ensuring the quality, safety, and efficacy of herbal medicines (HMs). Their broad distribution, biological activities, and detectability make them ideal for this role. This comprehensive review critically examines current trends and analytical perspectives regarding flavonoids in HM quality control. We first explore advanced quality control strategies that move beyond single-compound quantification, including chemical fingerprinting, metabolomics, network pharmacology, and the innovative concept of Q-markers. The review then provides an in-depth analysis of the analytical techniques central to flavonoid analysis, from the routine use of HPTLC and HPLC-UV to advanced hyphenated systems like UHPLC-QTOF-MS, highlighting their applications in authentication, standardization, and adulteration detection. Furthermore, we emphasize the growing importance of modern data analysis workflows, particularly the integration of chemometrics and molecular networking, for interpreting complex datasets and identifying robust, bioactivity-relevant markers. By synthesizing recent research (2017–2024), this work underscores a paradigm shift towards holistic, multi-marker approaches and data-driven methodologies. It concludes that the synergistic application of advanced analytical techniques with sophisticated data modeling is essential for the future of HM quality control, ensuring reliable and standardized herbal products for global consumers. Full article
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21 pages, 963 KB  
Article
Expert Perspectives on Enhancing Analytical Methods for Multi-Ingredient Dietary Supplements (MIDS): A Qualitative Study
by Ingyeong Ko, Hae Jin Park, Kwang Suk Ko, Hyunsoo Kim and Jieun Oh
Foods 2025, 14(21), 3598; https://doi.org/10.3390/foods14213598 - 22 Oct 2025
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Abstract
The increasing demand for multi-ingredient dietary supplements (MIDS), driven by diverse consumer health needs, has introduced analytical challenges in product testing and quality control. These challenges stem from complex ingredient interactions, formulation variability, and the diverse physicochemical properties of the individual components. To [...] Read more.
The increasing demand for multi-ingredient dietary supplements (MIDS), driven by diverse consumer health needs, has introduced analytical challenges in product testing and quality control. These challenges stem from complex ingredient interactions, formulation variability, and the diverse physicochemical properties of the individual components. To examine these issues and explore practical solutions, this study employed semi-structured focus group interviews with 33 industry professionals and 10 analytical experts from academic and industry. Professionals reported major obstacles including the degradation or loss of trace components, interferences among ingredients, analytical difficulties with specific dosage forms, and the lack of standardized testing protocols. To mitigate these challenges, professionals reported implementing various combination strategies including substituting problematic raw materials and modifying analytical instruments and pretreatment procedures, in order to improve test reproducibility. These measures were developed internally and varied significantly across companies, reflecting the absence of a unified analytical framework for MIDS testing. Building on these insights, the analytical experts proposed systematic improvements including developing matrix-specific pretreatment protocols and optimized extraction strategies as well as regulatory harmonization to enhance analytical reliability and reproducibility. These findings provide critical insights into current field practices and inform the development of standardized methodologies for the analysis and quality assurance of MIDS. Full article
(This article belongs to the Section Nutraceuticals, Functional Foods, and Novel Foods)
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