Search Results (100)

Camelina sativa sprouts were investigated as a source of glucosinolates (GLSs), and their modulation in response to germination time and elicitor treatments was evaluated. Total GLS content significantly decreased during sprouting, from 21.18 µmol/g DW in seeds to 0.75 µmol/g DW at 24 days, with 5-day-old sprouts selected as the optimal harvest stage, with 8.82 ± 0.08 µmol g⁻¹ DW. HPLC analysis identified three major aliphatic GLSs (GLS9, GLS10, GLS11), with GLS10 being the most abundant. Prior to treatment, tolerance assays showed that glucose (75 mM) and DL-methionine (2.5 mM) significantly increased total GLS content, whereas sulfur supplementation had no effect. Genotype-dependent responses were observed among Calena, Alan, and Pearl sprouts. Two-way ANOVA revealed a significant interaction between genotype and elicitor for total GLS content. Glucose and DL-methionine enhanced GLS accumulation in a cultivar-specific manner, with DL-methionine being more effective in Pearl, while Calena and Alan were more responsive to glucose. Sulfur treatments did not induce GLS accumulation in any genotype or condition tested. At the individual compound level, GLS9 was consistently increased by both elicitors, whereas GLS10 and GLS11 showed genotype and treatment-specific responses. Overall, these findings highlight the potential to increase the total GLS content in camelina sprouts through targeted elicitation and cultivar selection. By optimizing elicitor type, concentration, and timing, camelina sprouts could become a richer source of bioactive compounds. Full article

Broccoli (Brassica oleracea L. var. Italica) is a rich source of glucosinolates, bioactive compounds whose levels are markedly affected by postharvest senescence. The present study evaluated the effects of hormonal regulators associated with senescence—cytokinins, ethylene, and the ethylene-action inhibitor 1-methylcyclopropene (1-MCP)—on glucosinolate metabolism during postharvest storage of broccoli heads. Broccoli heads were treated with 6-benzylaminopurine (BAP), ethephon, or 1-MCP and stored at 20 °C for five days in darkness. Senescence progression was evaluated through superficial color and chlorophyll content. Glucosinolate pattern and gene expression involved in glucosinolate biosynthesis and degradation was also analyzed. Postharvest storage induced rapid yellowing, chlorophyll loss, and a general decline in glucosinolate content in control samples. BAP and 1-MCP treatments significantly delayed senescence, preserved chlorophyll levels, and reduced glucosinolate losses, particularly for indolic glucosinolates such as glucobrassicin and neoglucobrassicin. In the case of BAP-treated samples, glucobrassicin and neoglucobrassicin contents were 117% and 93% higher, respectively, compared to the controls on day 5. In the case of 1-MCP-treated samples, greater differences were detected on day 3, with glucobrassicin and neoglucobrassicin levels 69% and 66% higher, respectively. In contrast, ethephon accelerated senescence and promoted a more pronounced decrease in both aliphatic and indolic glucosinolates. Gene expression analyses showed a general downregulation of aliphatic glucosinolate biosynthetic genes during storage, whereas indolic pathway genes exhibited hormone-dependent responses. Overall, the results indicate that senescence-associated hormonal regulation plays a central role in glucosinolate metabolism during broccoli postharvest storage, and that BAP and 1-MCP treatments are effective strategies to maintain nutritional quality by delaying senescence and glucosinolate degradation. Full article

Rapeseed (Brassica napus L.) is a globally important oilseed crop; however, its ‘double-low’ cultivars exhibit substantially reduced glucosinolate levels in vegetative tissues. To investigate whether UV-B radiation could be used to enhance glucosinolate accumulation, we systematically examined the modulation of glucosinolate profiles and associated biosynthetic pathways in leaves of the ‘double-low’ cultivar NY4 under white light (WL) supplemented with two UV-B intensities: low-intensity UV-B (UVBL, 0.1 W m⁻²) and high-intensity UV-B (UVBH, 0.4 W m⁻²). Rapeseed seedlings were treated for 21 days under a 16 h photoperiod, and leaf samples were collected at the end of the treatment period, with three biological replicates per condition. Compared with the WL control, UVBL significantly increased total glucosinolate content by 64.57%, driven predominantly by elevated accumulation of progoitrin and neoglucobrassicin. In contrast, UVBH reduced total glucosinolate levels but markedly elevated gluconasturtiin content. Transcriptome analysis revealed that UVBL upregulated key genes involved in glucosinolate biosynthesis (e.g., MAM, IPMDH, CYP79F1, and SOT17/18) and transcription factors (e.g., MYB28, MYB34, MYB51, and MYB122). Conversely, UVBH downregulated genes associated with side-chain elongation of aliphatic glucosinolates and secondary modification of indolic glucosinolate. Collectively, these results demonstrate that low-intensity UV-B radiation can effectively boost total glucosinolate content in rapeseed leaves via transcriptional reprogramming. Full article

Broccoli is rich in glucosinolates (GSLs), secondary metabolites that contribute to both plant defense and human health. Optimizing the composition of major aliphatic GSLs is an important breeding objective, yet robust molecular markers for marker-assisted selection (MAS) remain limited. In this study, candidate gene-based kompetitive allele-specific PCR (KASP) markers were developed from conserved GSL biosynthesis genes, focusing on AOP2 and GSL-OH selected from 19 GSL-related genes. Marker–trait associations were evaluated in a natural broccoli population and further validated in an independent F₂ population. Among the tested markers, S101, located in AOP2, exhibited consistent genotype-dependent effects on GNA and PRO across both populations, supporting its stable predictive value. Receiver operating characteristic (ROC) analysis further confirmed strong classification performance of S101 for distinguishing high- and low-content genotypes of these traits in the F₂ population. In contrast, S074 and S035 showed population-dependent effects, with significant associations detected only in the natural population. Although association signals were reduced under mixed linear model (MLM) analysis with false discovery rate (FDR) correction, major loci identified under the general linear model (GLM) framework remained detectable. Overall, these results demonstrate the potential of candidate gene-based KASP markers for improving aliphatic GSL composition in broccoli through marker-assisted selection. Full article

Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot, has a significant impact on cabbage production worldwide. The goal of this research was to evaluate the effect of preventive foliar treatments with Bacillus velezensis strain RD-FC 88 on the primary and secondary metabolism of Xcc-infected cabbage cv. Futoški plants. Special attention was given to measuring metabolites’ changes, aiming to determine the influence of the applied biocontrol treatment on the development of plant immune response and resistance to pathogen. This study reports the first comprehensive biochemical and physiological analysis of the interaction between host plant, biocontrol strain and pathogen, thus providing novel insight into black rot management. Pathogen inoculation caused a significant decrease in the majority of measured metabolites, including most free amino acids (Gln, Ala, BCAA), phenolics, and glucosinolates. Preventive application of B. velezensis strain in Xcc-infected plants restored the levels of aromatic amino acids, Asp, Glu, Leu, Val, and Ala to control values. A similar pattern was observed in aliphatic glucosinolates sinigrin and glucoiberin, as well as for the indolic glucosinolate 4-methoxy-glucobrassicin. Additionally, increased accumulation of hydroxybenzoic acids, hydroxycinnamic acids, and kaempferol derivatives was also observed in the plants treated with the biocontrol strain and subsequently infected with Xcc, compared to plants solely infected with Xcc. The obtained results imply that the RD-FC 88 strain holds potential as an efficient priming agent, capable of stimulating cabbage cv. Futoški defense responses and enhancing its resistance to Xcc. Full article

Drought stress induces pronounced metabolic and transcriptional reprogramming of glucosinolate (GLS) biosynthesis in Brassica oleracea. An integrative approach combining HPLC-based quantification of individual GLSs, quantitative real-time PCR of core biosynthetic and regulatory genes, correlation-based network analysis, and in silico promoter characterization was applied to evaluate drought responses across genetically diverse accessions. Drought triggered strong, accession-specific shifts in GLS composition, with sinigrin content increasing from 35.9% to 55.1% in BR1 and glucoerucin reaching up to 80.2% in CCP1, while indolic GLSs such as glucobrassicin and neoglucobrassicin accounted for >75% of total GLSs in CV2 and CCP3. Hierarchical clustering separated accessions into four distinct drought response clusters independent of morphotype. Correlation analysis revealed drought-induced rewiring of GLS interdependencies, characterized by strengthened positive associations among aliphatic GLSs (r > 0.75). Gene expression profiling identified conserved MYB-centered regulatory modules (MYB28, MYB29, MYB34, MYB122) alongside strong accession-specific induction of CYP79F1 (up to 6.3-fold), FMOGS-OX5 (up to 4.8-fold), and ST5a (up to 5.1-fold). Promoter analysis revealed enrichment of ABA- and stress-responsive cis-regulatory elements. These findings delineate a genotype-dependent regulatory framework underlying GLS plasticity and identify quantitative metabolic and transcriptional markers relevant for breeding drought-resilient Brassica cultivars. Full article

Brassica juncea (L.) Coss. var. foliosa Bailey contains high glucosinolate (GSL) levels that define its flavor and defense properties. However, the regulatory mechanisms controlling GSL biosynthesis in Brassica crops remain unclear. Here, four MYB29 homologs were identified in allotetraploid Brassica juncea. These BjuMYB29 proteins localize to the nucleus and possess transcriptional activation activity. Evolutionary analysis suggests polyploidization-driven expansion of MYB genes contributed to GLS diversification in Brassica species. Expression profiling showed distinct spatiotemporal and herbivory-responsive patterns among BjuMYB29 homologs. Heterologous expression of BjuA03.MYB29 and BjuA10.MYB29 in Arabidopsis enhanced insect resistance via GSL accumulation. Although both homologs upregulate aliphatic GSL biosynthetic genes, they differentially regulate indolic GSLs, with BjuA03.MYB29 suppressing and BjuA10.MYB29 enhancing their accumulation, potentially through differential control of CYP79B2. These results reveal subfunctionalization among MYB29 homologs in GSL regulation. This functional diversification of MYB29 homologs offers novel targets for precision breeding of Brassica crops with customized GSL profiles to optimize pest resistance and nutritional quality. Full article

The toxicity of glucosinolate, isothiocyanate and sinapin limits canola meal’s use as non-ruminant animal feed. While monoculture microbial biorefining has been explored, the potential and capability of insect-associated microbiomes in this context remain underexplored. Herein, we extracted the gut and frass extracts from canola feeding larvae of Heliothis moth (HP), cabbage white (WCF) and cabbage looper (CL). Canola meal was fermented for one week with these extracts, followed by liquid chromatography–mass spectrometry (LC-MS)-assisted metabolomics analysis. Elevated branched-SCFAs 2-hydroxy butyrate and 3-hydroxy butyrate and propionate were observed in HP and WCF ferments, respectively. Aliphatic glucosinolates and sinapins showed ≥2-fold depletion in the HP and WCF frass ferments. In gut extract and frass-fermented canola meal, particularly of the HP group, tryptophan, tyrosine, and cysteine and glutathione metabolism were the most impactful pathways, aiding biogenic amine and branched-SCFA synthesis. S-adenosyl methionine (SAM) led salvaging, playing a key role in amino acid recycling via mercapturate metabolism, oxidative stress handling via the methionine and cysteine metabolism pathway, and sinapin metabolism through syringate degradation. These findings highlight the metabolic mechanism of brassica herbivore insect gut microbiome in detoxifying and adding value to canola meal. Such microbial communities have the potential to upcycle canola meal into a nutrient-rich feed additive with gut-health-promoting properties. Full article

Glucosinolates are secondary metabolites of the Brassicales, playing a role in plant protection and as health-promoting compounds. Here, Na₂SO₄ was used to modulate the aliphatic glucosinolate content in different organs of Eruca sativa Mill. In flowers, which accumulate the highest amount of glucosinolates, Na₂SO₄ increased the concentration of glucoraphanin, in roots of glucoerucin and in apical leaves it doubled the amount of dimeric 4-mercaptobutyl glucosinolate. The biosynthetic gene Branched-Chain Aminotransferase 4 was also induced in roots at the highest salt concentration, while in leaves all tested genes biosynthetic genes were downregulated or unaffected. Cytochromes P450 83A1 monooxygenase was downregulated at the highest salt concentration in all organs. Overall, E. sativa is a reliable source of glucosinolates, which can be modulated with Na₂SO₄. Full article

The quest to establish permanent outposts in space, the Moon, and Mars requires growing plants for nutrition, water purification, and carbon/nutrient recycling, as well as the psychological well-being of crews and personnel on extra-terrestrial platforms/outposts. To achieve these essential goals, the safety, quality, and sustainability of plant material grown in space should be comparable to Earth-grown crops. In this study, radish plants were grown at 2500 ppm CO₂ in two successive grow-outs on the International Space Station and at similar CO₂ partial pressure at the Kennedy Space Center. An additional control experiment was performed at the University of Louisiana Lafayette laboratory, at ambient CO₂. Subsequent analyses of glucosinolate and sugar species and content showed that regardless of growth condition, glucoraphasatin, glucoraphenin, glucoerucin, glucobrassicin, 4-hydroxyglucobrassicin, 4-methoxyglucobrassicin, and three aliphatic GSLs tentatively assigned to 3-methylpentyl GSL, 4-methylpentyl GSL, and n-hexyl GSL were present in all examined plants. The most common sugars were fructose, glucose, and sucrose, but some plants also contained galactose, maltose, rhamnose, and trehalose. The variability of individual secondary metabolite abundances was not related to gravity conditions but appeared more sensitive to CO₂ concentration. No indication was found that radish cultivation in space resulted in stress(es) that increased glucosinolate secondary metabolism. Flavor and nutrient components in space-grown plants were comparable to cultivation on Earth. Full article

Neurodegenerative, neurodevelopmental, and psychiatric disorders, as well as epilepsy, affect millions of people. Due to their impact on patients’ quality of life, they represent a major health issue. Natural compounds are arising as new treatments for these diseases. Particularly, glucosinolates (GLS) are secondary metabolites found in Cruciferae family plants. Their basic structure consists of a glucose unit linked to a thiohydroximate-O-sulfonate group and an aliphatic, aralkyl, or indolyl side chain, depending on their precursor amino acid. Specifically, aliphatic GLS derive from methionine, aromatic ones from phenylalanine, and indolic ones from tryptophan. Myrosinase (thioglucoside glucohydrolase) is the crucial enzyme for GLS degradation, leading to the production of isothiocyanates (ITCs). ITCs attracted considerable scientific interest for their protective effects against various diseases, thanks to their antioxidant, anti-inflammatory, and neuroprotective properties. Here, we collected the latest evidence regarding ITC effects in neurodegenerative, neurodevelopmental, and psychiatric disorders, including preclinical and clinical studies published in the last decade. These studies evidenced ITCs’ neuroprotective effects, exerted mainly through antioxidant and anti-inflammatory mechanisms. Thus, ITCs’ integration, also through the diet, may represent a safe and efficacious strategy to improve health and limit the risk of neurological and psychiatric disorders. However, new large-scale trials are needed to determine their therapeutic potential, particularly for diseases with no clinical evidence. Full article

(1) Background: At the time of harvest, the stems of broccoli are frequently discarded. (2) Methods: In this study, the sulforaphane content and glucosinolate profile of broccoli stems were analyzed at different temperature treatments. (3) Results: Thermal treatment of broccoli stems for 1 h resulted in maximal sulforaphane content at 50 °C, with a subsequent progressive reduction in concentration correlating to elevated temperatures. Metabolomic analysis was conducted on broccoli stem samples subjected to 25 °C (CK), 50 °C, and 80 °C treatments. Among the 25 identified GSLs, the 50 °C-treated samples demonstrated significantly reduced GSL accumulation, whereas the 80 °C group exhibited maximal GSL retention. Indole derivatives predominated among the three GSL subclasses (aliphatic, aromatic, and indole), accounting for approximately 70% of total GSLs across all groups. The observed GSL depletion at 50 °C correlated with enhanced sulforaphane biosynthesis. Comparative analysis further indicated that 80 °C treatment induced a more pronounced elevation of indole GSLs compared to aliphatic and aromatic counterparts in broccoli stems. (4) Conclusions: The results demonstrated that indole GSLs in broccoli stems exhibit superior thermal stability. Moderate thermal treatments effectively enhance sulforaphane content, whereas exposure to 80 °C significantly increases total GSL content. Full article

While a balanced diet can fulfill most nutritional needs, optimizing the composition of specific foods like broccoli can amplify their health benefits. Background/Objectives: Broccoli (Brassica oleracea L. Italica group) is a widely consumed cruciferous vegetable valued for its gastrointestinal and immune health benefits. However, the individual contributions and interactions of broccoli glucosinolates, as they hydrolyze into bioactive isothiocyanates, remain poorly understood. Methods: This study investigated mixtures of four major aliphatic glucosinolates—glucoraphanin, gluconapin, progoitrin, and sinigrin—in individual and combinational models to assess their effects on human colorectal cell proliferation. Results: Combination index analysis revealed moderate to strong antagonistic interactions among these glucosinolates, with the most significant antagonism observed during enzymatic hydrolysis by myrosinase. Mixture analysis identified an optimal glucosinolate ratio including glucoraphanin (81–84%), gluconapin (9–19%), and others (0–7%) to maximize their antiproliferative effects (adjusted R² > 0.80). This optimal profile was achievable within the target broccoli mapping population. Testing the near-optimal VB067 isogenic broccoli line showed a 44% increase in antiproliferative activity compared to the initial breeding parent or an average sister line. Conclusions: This study highlights the potential of leveraging nutrient–nutrient interactions to guide molecular breeding and produce functional varieties of cruciferous vegetables with optimized health benefits. Full article

Glucosinolates (GSLs) are nitrogen/sulfur-containing glycosides widely present in the order of Brassicales, particularly in the Brassicaceae family. Camelina (Camelina sativa (L.) Crantz) is an oilseed plant belonging to this family. Its seeds, in addition to a distinctive fatty acid composition, contain three aliphatic GSLs: glucoarabin, glucocamelinin, and homoglucocamelinin. Our study explored the impact of these GSLs purified from Camelina press cake, a by-product of Camelina oil production, on yeast chronological aging, which is the established model for simulating the aging of post-mitotic quiescent mammalian cells. Supplementing yeast cells with GSLs extends the chronological lifespan (CLS) in a dose-dependent manner. This enhancement relies on an improved mitochondrial respiration efficiency, resulting in a drastic decrease of superoxide anion levels and an increase in ATP production. Furthermore, GSL supplementation affects carbon metabolism. In particular, GSLs support the pro-longevity preservation of TCA cycle enzymatic activities and enhanced glycerol catabolism. These changes contribute positively to the phosphorylating respiration and to an increase in trehalose storage: both of which are longevity-promoting prerequisites. Full article

Soil-borne diseases threaten sustainable agriculture, traditionally managed by chemical fumigants, whose use is now restricted due to environmental and health concerns. This study evaluates the biofumigation potential of Brassicaceae species, specifically Brassica carinata A. Braun., Brassica juncea (L.) Vassiliĭ Matveievitch Czernajew., Raphanus sativus L., and Sinapis alba L., cultivated in central Spain. Field trials across two growing cycles assessed biomass production, glucosinolate (GSL) concentration, photosynthetically active radiation (PAR) interception, and radiation use efficiency (RUE). Biomass production varied across species and sampling dates, with S. alba and R. sativus outperforming other species in shorter cycles, while B. juncea and B. carinata showed a more efficient GSL profile regarding soil-borne disease control, particularly in aliphatic GSLs like sinigrin. Results highlight B. juncea and B. carinata as potent biofumigants due to their high GSL levels, whereas S. alba and R. sativus are more suited to early biomass production. The study also explores the chlorophyll content index (SPAD) as a potential field indicator of GSL concentration, providing a practical approach for optimizing biofumigation timing. These findings support the selection of specific Brassicaceae species adapted to climatic conditions and crop cycles for effective biofumigation in sustainable agricultural practices. Full article