Search Results (464)

HDAC8 is a histone deacetylase enzyme that plays a key role in the development of various diseases in humans, including cancers, neurodegenerative diseases, and alcohol use disorder. Although HDAC8 is classified as a Zn²⁺-dependent metalloenzyme, available data regarding the affinity of other biologically relevant ions, such as Fe²⁺, Ni²⁺, Co²⁺, and Mg²⁺, for the HDAC8 enzyme active site remain unclear and contradictory. The mechanism by which these ions compete with Zn²⁺ for the HDAC8 active site is not well understood. In this study, we performed density functional theory (DFT) calculations at the B3LYP/6-31+G(d) level of theory, combined with polarizable continuum model computations (PCM) in water (ε = 78) and methanol (ε = 32). The results show that Zn²⁺ remains the thermodynamically preferred cofactor across all modeled reactions. Although Fe²⁺ and Co²⁺ gain partial stabilization upon increasing coordination number, the associated entropic and desolvation penalties prevent them from outcompeting Zn²⁺ under physiologically relevant conditions. Only a limited number of substitution reactions for Fe²⁺ and Co²⁺ yield ∆G values near thermodynamic neutrality, and only in specific coordination states. In contrast, all modeled Ni²⁺ substitution reactions are unfavorable, and Mg²⁺ is strongly excluded from the HDAC8 active site in all reactions. The resulting metal preference hierarchy—Zn²⁺ > Co²⁺ ≈ Fe²⁺ > Ni²⁺ > Mg²⁺—supports experimental observations and clarifies the intrinsic selectivity of the HDAC8 enzyme towards Zn²⁺. These insights provide a molecular basis for understanding HDAC8 metallo-regulation and may guide the rational design of novel, isoform-specific HDACi with improved binding properties. Full article

Background/Objectives: Since 1988, the IARC has classified alcohol as a type 1 carcinogen, causally linked to seven types of cancer (oral cavity, pharynx, larynx, esophagus, colorectum, liver and breast carcinomas). Several agencies, such as the WHO and the IARC, hold that there is a direct monotonic association between any gram of alcohol consumed and the risk of cancer, regardless of the drinking pattern. On the other hand, an expanding body of evidence indicates that drinking pattern may substantially modify the effect of alcohol consumption. The Mediterranean alcohol-drinking pattern (MADP) includes different aspects of alcohol consumption, such as preference for red wine, moderate alcohol consumption with meals, spreading consumption over the week and avoiding binge drinking. Conformity to this pattern has shown inverse associations with all-cause mortality, cardiovascular disease and diabetes. However, its relationship with cancer incidence has not been studied yet. Our objective was to assess how alcohol consumption patterns, with particular emphasis on the MADP, relate to the incidence of the seven alcohol-related cancers. This information is needed to support cancer prevention recommendations that may go beyond the amount of alcohol consumed to also include the drinking pattern. Methods: We prospectively followed 19,541 participants in the SUN (“Seguimiento Universidad de Navarra”) cohort for a median of 13.8 years. We classified participants into four groups, namely, abstainers and three further groups according to their adherence to the MADP score (low, moderate and high). Results: A substantial reduction in the risk of alcohol-related cancer incidence was observed only in men for high versus low adherence to the MADP, with an adjusted hazard ratio (HR) of 0.44 (95% confidence intervals (CIs) (0.21–0.92)). The category of moderate adherence to the MADP showed a lower risk of cancer incidence with a tendency towards statistical significance (HR = 0.56, 95% CI, 0.30–1.06). For women, no result reached statistical significance. Conclusions: Based on the available evidence, separate messages by sex should be delivered. In men, the association between alcohol and cancer goes beyond the amount of alcohol consumed, and a Mediterranean drinking pattern may be beneficial even for alcohol-related cancers. Men should, therefore, receive an additional message: among alcohol consumers, greater adherence to the MADP may help lower their risk of developing alcohol-related cancers. No benefit is supported for the MADP against alcohol-related cancers in women. Full article

The growing interest in no- and low-alcohol (NoLo) wines reflects evolving consumer preferences toward moderation, health, and mindful drinking. This study investigates consumer perception and acceptance of NoLo wines within a traditional wine context (Trentino-Alto Adige, Italy), based on a survey of 344 people. Respondents were primarily between 18 and 34 years old. Descriptive results indicated low familiarity and purchase frequency but positive attitudes, especially among women and health-oriented consumers. Nonparametric tests revealed that gender significantly affected familiarity, social acceptance, and willingness to recommend NoLo wines, with women respondents showing higher engagement and acceptance. Age showed a weaker but still significant effect on familiarity, while consumers who regularly consumed NoLo beverages exhibited greater social acceptance and willingness to recommend. In addition, logit and probit models suggested that preference for mid-to-low alcohol levels and prior experience with alcohol-free drinks positively influenced purchase frequency. In contrast, traditional wine consumption habits and expenditure had no significant effects. These findings suggest that while NoLo wine adoption in a premium wine region such as Trentino-Alto Adige is in an emerging phase, it is underpinned by evolving young consumer motivations toward moderation, well-being, and social inclusivity, offering clear opportunities for targeted market development and product innovation. Full article

Alcohol level is a critical quality parameter in Chinese baijiu, significantly influencing its flavor profile, sensory characteristics, and overall quality, which in turn affect consumer preferences and the development of the liquor industry. Understanding the non-volatile compounds in strong-flavor baijiu (SFB) is essential for elucidating its taste and mouthfeel characteristics. This study aims to identify non-volatile compounds using a non-targeted metabolomics approach and investigate the differences between high- and low-alcohol SFB from the Jiangsu region. A total of 647 non-volatile compounds were quantified. The key differential metabolites were screened among different samples. In total, 110 key differential non-volatile compounds were identified and quantified, which displayed significant differences between high- and low-alcohol SFB samples and showed notable similarities in compound types. Furthermore, the variation in non-volatile profiles among samples of the same brand but different alcohol levels was investigated. The different brands and origins of SFB samples were identified using OPLS-DA. The results indicated that the key non-volatile metabolites in most of the high-alcohol samples were higher than those in low-alcohol samples. This study provides valuable insights into the impact of alcohol level on the non-volatile composition of SFB, offering a theoretical foundation for flavor characterization, quality control, and standardization in baijiu production. Full article

Orange peel waste has potential to be valorized from agro-industrial and food sectors to formulate products for personal hygiene and public health. This study presents the formulation of alcohol-based antibacterial gels incorporating essential oils extracted from Citrus sinensis orange peel waste and its sensory evaluation among 770 participants in a holistic approach. The orange essential oil, obtained via hydrodistillation, demonstrated a high limonene content of 96.5% by GC-MS. Antibacterial activity assessed by agar diffusion assays showed orange essential oil efficacy against Escherichia coli and Staphylococcus aureus, with inhibition zones of 25.9 mm and 23.62 mm, respectively. Two gel prototypes, GSA and GSB, were developed and sensorily evaluated. GSA was preferred for its superior appearance, spreadability, absorption, and smell, with 99% acceptability. Appearance and spread sensory parameters were the differentiators between both formulations according to user preferences. Thus, 93% of respondents are willing to use either GSA or GSB as a daily hygiene product over commercial ones. Although the gels exhibited reduced antibacterial activity relative to essential oil, with inhibition zones measuring 8.3 mm for E. coli and 9.0 mm for S. aureus, they retained satisfactory user acceptability. These findings support the use of citrus biowaste-derived essential oils in sustainable personal hygiene products. Full article

Second-grade watermelons are a lycopene-rich by-product currently discarded despite their potential for valorization. This study aimed to develop a consumer-accepted alcoholic liqueur by alcoholic maceration of watermelon pulp, optimizing the formulation for lycopene retention, antioxidant activity, and sensory quality. Three pulp ratios (15, 20, and 25% v/v) were macerated in 39% v/v cane alcohol for 5 days at 20 °C. The 20% pulp formulation (T2) achieved the best balance of lycopene (8.6 mg L⁻¹), DPPH antioxidant activity (580 µmol TE L⁻¹), redness (CIE a* = 14.1), and overall acceptability (7.6/9). Under nitrogen-flushed amber glass, T2 retained ≥82% lycopene and ≤10% antioxidant loss after 90 days at 20 °C, following first-order degradation kinetics (Ea = 43 kJ mol⁻¹). A minimum shelf-life of three months is projected under these experimental conditions. Life-cycle screening indicates 0.84 kg CO₂-eq L⁻¹, with a 1.9-year payback and 1.7 t CO₂-eq savings per ton of rescued fruit compared to landfill. These findings demonstrate the feasibility of producing a shelf-stable, naturally colored craft spirit with watermelon flavor, aligning with consumer preferences and circular economy principles in the alcoholic beverage sector. Full article

Background: In Siberian folk medicine, Sagan-Dalya (Rhododendron adamsii Rehder) of the Ericaceae family is used as a tonic and restorative in the form of infusions and decoctions. Pharmacological studies have shown that alcoholic extracts of this plant enhance performance and have anti-inflammatory and immunomodulatory effects. Rhododendron adamsii shoots accumulate essential oil (up to 1.6%), flavonoids (1.8–3.0%), tannins (up to 6.9%), phenolic carbolic acids, β-sitosterin, oleanolic and ursolic acids, simple phenolic compounds, and coumarins. Methods: Supercritical carbon dioxide extraction (SC-CO₂) is the most preferred environmentally friendly and selective method for extracting these natural compounds from the plant matrix of Rh. adamsii due to their high thermolability. Tandem mass spectrometry was applied to detect chemical compounds. Mass-spectrometry (MS) analysis was performed on an ion trap equipped with an ESI source in negative and positive ion modes. The capture rate was one spectrum/s for MS and two spectrum/s for MS/MS. All experiments were repeated three times. A four-stage ion separation mode (MS/MS mode) was implemented. Results: The operative parameters and working conditions have been optimized by different pressure (100–400 bars) and temperature (31–70 °C) regimes, and CO₂ flow rate (10–25 mL/min) with 1 C₂H₅OH as a co-solvent. The extraction time varied from 60 to 90 min. The maximum global yield of biologically active substances (BAS) from R. adamsii leaves and stems was observed under the following extraction conditions: Pressure: 350 bar, extraction temperature: 65 °C, extraction time: 1 h; the global yield of BAS was 8.5 mg/g of plant sample; the share of the co-solvent (C₂H₅OH) was 2%. In total, forty-nine different BAS were identified in the Rh. adamsii SC-CO₂ extracts. Conclusions: The obtained results may shed new light on the scientific basis for the traditional medicinal use of Rh. adamsii leaf and stem extracts. The pharmacological contribution of the identified phytocannabinoids requires further detailed study. It is hypothesized that the excellent transdermal permeability of supercritical extracts may open new therapeutic approaches using transdermal formulations based on SC-CO₂ extracts of Rh. adamsii. Full article

Chuanbai Rex (CR) and New Zealand white (NZ) rabbits are highly popular and widely produced for meat consumption in Sichuan, China. But comparative studies on nutritional and sensory qualities of meat from two breeds are still lacking. This study integrated lipidomic and volatilomic approaches to elucidate the breed-specific differences in the longissimus dorsi between CR and NZ (n = 5 per breed). Analysis of fatty acid composition revealed that CR had lower content of PUFA but with a more balanced n-6/n-3 PUFA ratio compared to NZ. LC-MS-based lipidomics identified 52 significantly different lipids between the two breeds, and CR had higher levels of phospholipids and sphingolipids, while NZ was richer in triglycerides and diglycerides. SPME-GC-MS analysis identified a total of 140 VOCs, including primarily aldehydes (>50%), alcohols (<20%), and hydrocarbons (<15%). CR contained unique aroma compounds such as acetoin and 2-(E)-heptenal, while NZ had more of nonanal and pentadecane. Pearson correlation analysis performed between differential lipid metabolites and characteristic VOCs showed that 22 lipid biomarkers were significantly correlated with seven key VOCs, suggesting breed-distinct pattern for flavor formation. Albeit a small sample size, this study provides preliminary insights into biochemical mechanisms determining rabbit meat quality and offers a scientific basis for developing premium rabbit meat products tailored to consumer preferences. Full article

The processing technique critically determines the quality of prepackaged braised meat products. This study aimed to evaluate the effects of an innovative processing method against traditional methods on the product’s shelf-life and quality attributes. Results: no significant difference in shelf-life was observed between the experimental and control groups. However, the innovative method significantly improved product quality. The experimental group exhibited a redder and bluer color, significantly higher hardness (2–4 times, p < 0.01) and chewiness, alongside better moisture retention and meat yield. Sensory evaluation confirmed an overall preference for the experimental group (p < 0.05). Flavor profile analysis revealed a greater number and more stable retention of key flavor compounds (alcohols, ketones, and ethers) in the experimental group. The innovative processing method optimizes traditional techniques by significantly enhancing the physicochemical, textural, sensory, and flavor properties of prepackaged braised meat, without compromising shelf-life, providing a novel strategy for producing high-quality products. Full article

Binge drinking is defined as consuming a large amount of alcohol in a short period of time, whereas hangover is a cluster of unpleasant mental symptoms and physical signs that typically manifest the next day after binge drinking. Binge drinking is a prevalent pattern of alcohol consumption, especially in adolescents, with dualistic effects on social behavior. While some studies demonstrate that a single episode of binge drinking enhances sociability and preference for social novelty, other studies indicate that repeating cycles of binge drinking and hangover can lead to persistent negative affect and consequently social withdawal. This is an integrative narrative review synthesizing human studies and animal models of binge drinking (also known as alcohol intoxication) and hangover (also known as alcohol withdrawal). The major databases consulted were PubMed, Scopus, and Web of Science. The search terms used were “binge drinking” or “hangover”and “social behavior” or “social brain” in combination with “rats”, “mice” or “humans”. Finding the missing link between structural and functional changes in the social brain in the context of binge drinking and hangover is crucial for developing novel therapeutic strategies for alcohol intoxication and withdrawal. This review focuses on changes in hypothalamic neurohormones and extrahypothalamic neurotransmitters in these states, and concludes with the statement that targeting neuropeptides such as corticotropin-releasing hormone (CRH) and arginine vasopressin (AVP) and their receptors, which are involved in both binge drinking and social behavior, may prevent repeated cycles of binge drinking and hangover from spiraling into alcohol addiction and, ultimately, social isolation. Full article

Fructophilic lactic acid bacteria (FLAB), Apilactobacillus kunkeei strains AG8 and AG9 were selected in the current study for in-depth analysis. Cultivation on fructose yeast peptone (FYP) medium with varying fructose concentrations (1%, 10%, and 30%) revealed that higher fructose levels promoted acetate production over lactate, confirming a heterofermentative metabolic profile. Ethanol production was negligible, consistent with the absence of alcohol dehydrogenase (ADH) activity. Enzyme assays showed fructokinase activity doubled at 30% fructose, while acetate kinase activity increased and L-lactate dehydrogenase activity decreased. This shift in enzyme ratios from 1:1 at 1% fructose to 10:1 or 15:1 at higher concentrations explains the metabolic preference for acetate. Apb. kunkeei is an obligate FLAB, growing poorly on glucose unless supplemented with external electron acceptors like pyruvate or oxygen. It lacks ADH, but retains acetaldehyde dehydrogenase (ALDH), enabling acetate production and additional ATP generation, enhancing biomass yield. The absence of the adhE gene contributes to NAD+/NADH imbalance and favors acetate production. Gene expression studies targeting fructose transport enzymes showed elevated expression of ABC transporters and carbohydrate metabolism genes in response to fructose. ADH expression remained low across sugar concentrations. Fructokinase gene expression was shown to be strain specific. Neither strain expressed the ABC transporter ATP-binding protein gene on glucose, nor the bacteriocin ABC transporter gene, correlating with the absence of antibacterial activity. These findings underscore the metabolic specialization of Apb. kunkeei, its reliance on fructose, and the role of ABC transporters in optimizing fermentation. The strain-specific gene expression and metabolic flexibility highlight its potential as a probiotic and feed additive in apiculture and biotechnology. Full article

Beer consumption patterns are evolving, with gluten-free beers (GFBs) and low- and no-alcohol beers (NABLABs) exhibiting continuous growth, underpinned by health-conscious consumers, younger generations’ preference for moderate drinking, and a rising awareness of gluten intolerance. This study investigates whether online search behaviour reflects these market changes and anticipates future consumption trends. A combined methodological framework was applied, integrating time-series analysis of Google Trends data—based on a decomposition model with a five-year forecast—with descriptive and semantic insights from Search Engine Optimisation (SEO) techniques using the specialised SEOZoom platform. The statistical decomposition enabled the identification of long-term trends, cyclical variations, and seasonal patterns in user interest. Italy was selected as a case study, representing a market where these niche segments have grown considerably despite a traditionally limited craft beer culture. The results reveal a steady rise in online interest in both GFB and NABLAB; GFB searches are primarily linked to health and dietary concerns, whilst NABLAB queries encompass a broader set of topics, including physiology, legislation, and consumption contexts. The forecasts confirm the persistence of this positive trend over the next five years. The approach demonstrates the potential of integrating digital and statistical tools to monitor emerging consumption dynamics and guide strategic decisions in the beverage sector. Full article

(1) Background: This article reviews the biological characteristics of phenolic acid compounds, focusing on their mechanisms of action in various liver diseases. (2) Methods: The review adheres to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. We utilized PubMed and Web of Science databases to search for relevant studies on the use of phenolic acids in liver diseases from 2015 to 2025. (3) Results: Phenolic acids can improve different types of liver diseases, including drug-induced liver injury (DILI), alcoholic liver disease (ALD), metabolic dysfunction-associated steatotic liver disease, liver fibrosis, and liver cancer. Their beneficial effects are attributed to mechanisms such as anti-inflammatory properties, antioxidant activity, regulation of lipid metabolism, inhibition of cell apoptosis, and modulation of gut microbiota. (4) Conclusion: Phenolic acids exhibit a good protective effect against various liver diseases and are associated with multiple signaling pathways. However, the primary target cells and specific molecular targets of phenolic acids remain unclear, necessitating further research to elucidate their protective mechanisms in liver diseases. Full article

Non-alcoholic fatty liver disease (NAFLD) is increasingly recognized as a systemic condition with neuropsychiatric comorbidities, including depression. Growing evidence for the neuroprotective, antidepressant, and anxiolytic potential of Portulaca oleracea (PO) extract, provides a compelling rationale for investigating its effects in the interaction between dietary models of NAFLD and vulnerability to stress-related disorders. Fifty-four 14- to 18-week-old male and female C57BL/6N mice were distributed in two equal groups and fed either a methionine- and choline-deficient diet (MCD) or a methionine- and choline-controlled diet (MC). Subsequently, half of each group was subjected to chronic unpredictable mild stress (CUMS) and PO treatment. MCD caused significant weight loss, whereas MC promoted weight gain. Behaviorally, MCD induced anhedonia- and anxiety-like behaviors, worsened by CUMS. MC diet reduced CUMS-induced anhedonia, though anxiety-like behavior emerged only under stress. Recognition memory was impaired in stressed MCD-fed mice, while MC-fed mice showed enhanced novel object preference. At the cellular level, MCD suppressed hippocampal microglia and caused cortical astrocyte dysfunction, whereas the MC diet promoted cortical neurogenesis potentiated through PO, abolished by chronic stress. These findings underscore the impact of dietary composition on PO’s systemic effects under chronic stress and support a mechanistic link between NAFLD-related dysfunction and depression-like phenotypes. Full article

Sake is a traditional Japanese alcoholic beverage that contains ethyl caproate (EC), a compound that enhances its economic value due to consumer preference. Procyanidins are polyphenolic compounds found in fruits such as apples, with procyanidin B2 (PB2) commonly used as a standard compound. Detecting EC and PB2 directly in intact or simply prepared beverages (e.g., dilution without organic solvent) requires liposomes as a model system. In this study, we focused on liquid-ordered/liquid-disordered liposomes, particularly reverse-domain liposomes, which were identified by fluorescence microscopy. EC and PB2 were found to increase and decrease the ratio of reverse-domain liposomes, respectively. The observation of cell-sized liposomes, combined with calibration curves, enabled a simplified analysis of EC and PB2. This approach has potential applications in the development, optimization, and practical implementation of food analytical methods for routine monitoring of beverage quality. These findings also provide a foundation for further studies on the biophysical and physiological properties of EC and PB2. Full article