Search Results (756)

In the context of green building development, the lighting design of campus living rooms in hot summer and cold winter areas faces the dual challenges of glare control in summer and insufficient daylight in winter. Based on BIM technology, this study uses Revit 2016 modeling and the HYBPA 2024 performance analysis platform to simulate and optimize the daylighting performance of the campus activity center of Hunan City College in multiple rounds of iterations. It is found that the traditional single large-area external window design leads to uneven lighting in 70% of the area, and the average value of the lighting coefficient is only 2.1%, which is lower than the national standard requirement of 3.3%. Through the introduction of the hybrid system of “side lighting + top light guide”, combined with adjustable inner louver shading, the optimized average value of the lighting coefficient is increased to 4.8%, the uniformity of indoor illuminance is increased from 0.35 to 0.68, the proportion of annual standard sunshine hours (≥300 lx) reaches 68.7%, and the energy consumption of the artificial lighting is reduced by 27.3%. Dynamic simulation shows that the uncomfortable glare index at noon on the summer solstice is reduced from 30.2 to 22.7, which meets the visual comfort requirements. The study confirms that the BIM-driven “static-dynamic” simulation coupling method can effectively address climate adaptability issues. However, it has limitations such as insufficient integration with international healthy building standards, insufficient accuracy of meteorological data, and simplification of indoor dynamic shading factors. Future research can focus on improving meteorological data accuracy, incorporating indoor dynamic factors, and exploring intelligent daylighting systems to deepen and expand the method, promote the integration of cross-standard evaluation systems, and provide a technical pathway for healthy lighting environment design in summer-hot and winter-cold regions. Full article

Advanced computer vision (CV) and deep learning (DL) are essential for sustainable agriculture via automated vegetation management. This paper methodically reviews advancements in these technologies for agricultural settings, analyzing their fundamental principles, designs, system integration, and practical applications. The amalgamation of transformer topologies with convolutional neural networks (CNNs) in models such as YOLO (You Only Look Once) and Mask R-CNN (Region-Based Convolutional Neural Network) markedly enhances target recognition and semantic segmentation. The integration of LiDAR (Light Detection and Ranging) with multispectral imagery significantly improves recognition accuracy in intricate situations. Moreover, the integration of deep learning models with control systems, which include laser modules, robotic arms, and precision spray nozzles, facilitates the development of intelligent robotic mowing systems that significantly diminish chemical herbicide consumption and enhance operational efficiency relative to conventional approaches. Significant obstacles persist, including restricted environmental adaptability, real-time processing limitations, and inadequate model generalization. Future directions entail the integration of varied data sources, the development of streamlined models, and the enhancement of intelligent decision-making systems, establishing a framework for the advancement of sustainable agricultural technology. Full article

Nowadays, with the accumulation of large amounts of stress-response transcriptomic data in plants, it is possible to clarify the key genes and transcription factors (TFs) involved in these processes. Here, we present the comprehensive transcriptomic meta-analysis of the high light (HL) response in photosynthetic tissues of Arabidopsis thaliana (L.) Heynh., offering new insights into adaptation mechanisms of plants to excessive light and involved gene regulatory networks. We analyzed 21 experiments covering 58 HL conditions in total, yielding 218,000 instances of differentially expressed genes (DEGs) corresponding to 19,000 unique genes. Based on these data, we developed the publicly accessible AraLightDEGs resource, which offers multiple search filters for experimental conditions and gene characteristics, and we conducted a detailed meta-analysis using our R pipeline, AraLightMeta. Our meta-analysis highlighted distinct transcriptional programs between short- and long-term HL responses in leaves, revealing novel regulatory interactions and refining the understanding of key DEGs. In particular, long-term HL adaptation involves key TFs such as CRF3 and PTF1 regulating antioxidant and jasmonate signaling; ATWHY2, WHY3, and emb2746 coordinating chloroplast and mitochondrial gene expression; AT2G28450 governing ribosome biogenesis; and AT4G12750 controlling methyltransferase activity. We integrated these findings into a conceptual scheme illustrating transcriptional regulation and signaling processes in leaf cells responding to long-term HL stress. Full article

Blueberries are valued for their flavor and health benefits, but inconsistent ripeness at harvest complicates post-harvest food processing such as sorting and quality control. To address this, we propose a lightweight convolutional neural network (CNN) to detect blueberry ripeness in complex field environments, supporting efficient and automated food processing workflows. To meet the low-power and low-resource demands of embedded systems used in smart processing lines, we introduce a Grouped Large Kernel Reparameterization (GLKRep) module. This design reduces computational cost while enhancing the model’s ability to recognize ripe blueberries under complex lighting and background conditions. We also propose a Unified Adaptive Multi-Scale Fusion (UMSF) detection head that adaptively integrates multi-scale features using a dynamic receptive field. This enables the model to detect blueberries of various sizes accurately, a common challenge in real-world harvests. During training, a Semantics-Aware IoU (SAIoU) loss function is used to improve the alignment between predicted and ground truth regions by emphasizing semantic consistency. The model achieves 98.1% accuracy with only 2.6M parameters, outperforming existing methods. Its high accuracy, compact size, and low computational load make it suitable for real-time deployment in embedded sorting and grading systems, bridging field detection and downstream food-processing tasks. Full article

Photophores are light-producing organs found in many fish species living in the mesopelagic, bathypelagic, and abyssal layers of the ocean. They function to attract prey, confuse predators, and communicate with other individuals of the same species. Understanding the structure and function of photophores is crucial to exploring bioluminescence and the ecological adaptations of marine life in deep-sea environments. The present study is the first to investigate the photophore anatomy of the mesopelagic fish Ichthyococcus ovatus (Cocco, 1838), using specimens naturally stranded along the coast of the Strait of Messina. The morphology of the ventral photophores of I. ovatus includes four functional parts: a tank containing photogenic cells, a lens filter, a reflector surrounding the entire organ, and a pigmented layer. An immunohistochemical assay was conducted using anti-nNOS and anti-S100p antibodies. The presence of nNOS/NOS type I immunolabeling the pigmented layer surrounding the photophores and the nerve fibers reaching the lens suggests a potential role of neuronal nitric oxide signaling in modulating light shielding by the pigment sheath, controlling light exposure, and adjusting light focusing though the lens-associated nerves. S100p immunostaining was observed in the nerve fibers reaching the photophores, highlighting its potential involvement in regulating neuronal calcium levels and, consequently, influencing signal transmission to control bioluminescence output. A sensory feedback pathway from the photophore to the CNS is suggested. Within the lens and in the irregularly shaped cells located in the photophore’s lens, S100p immunolabeling could indicate active signaling and differentiation processes. These findings expand our understanding of light-emitting systems in mesopelagic fishes and offer a valuable foundation for future studies on the functional and evolutionary significance of photophores. Full article

With the rapid advancement of computer vision and deep learning technologies, the accuracy and efficiency of real-time gesture recognition have significantly improved. This paper introduces a gesture-controlled robot system based on thermal imaging sensors. By replacing traditional physical button controls, this design significantly enhances the interactivity and operational convenience of human–machine interaction. First, a thermal imaging gesture dataset is collected using Python3.9. Compared to traditional RGB images, thermal imaging can better capture gesture details, especially in low-light conditions, thereby improving the robustness of gesture recognition. Subsequently, a neural network model is constructed and trained using Keras, and the model is then deployed to a microcontroller. This lightweight model design enables the gesture recognition system to operate on resource-constrained embedded devices, achieving real-time performance and high efficiency. In addition, using a standalone thermal sensor for gesture recognition avoids the complexity of multi-sensor fusion schemes, simplifies the system structure, reduces costs, and ensures real-time performance and stability. The final results demonstrate that the proposed design achieves a model test accuracy of 99.05%. In summary, through its gesture recognition capabilities—featuring high accuracy, low latency, non-contact interaction, and low-light adaptability—this design precisely meets the core demands for “convenient, safe, and natural interaction” in rehabilitation, smart homes, and elderly assistive devices, showcasing clear potential for practical scenario implementation. Full article

The complex systems approach to cognitive–motor processing values multifractal nonlinearity as a key formalism in understanding internal interactions across multiple scales that preserve adequate task-directed behaviors. By using a computer task with increasing difficulty, we focused on the potential link between the difficulty threshold during a task, assessed by the individual’s score ceiling, and the corresponding level of multifractal nonlinearity in movement behavior, assessed based on a time series of cursor displacements. Entropy-based multifractality (MF) and multifractal nonlinearity obtained using a t-test comparison between the original and linearized surrogate series (t_MF) of the time series characterized individual adaptive capacity. A time-varying increase in the score helped in assessing performance when facing increasing difficulty. Twenty-one participants performed a herding task (7 min), which involves keeping three moving sheep near the center of a screen by controlling the mouse pointer as a repelling shepherd dog. The more the score increased, the more the increased herd movement amplitude amplified task difficulty. The time course of the score, score dynamics (score-dyn), markedly diverged across participants, exhibiting a ceiling effect in some during the last third of the task (phase 3). This observation led us to arbitrarily distinguish three phases of the same duration and focus on phase 3, where marked differences in score-dyn emerged. Hierarchical clustering of principal components, starting with principal component analysis, identified three clusters among the participants: cluster 1 was defined by an underrepresentation of score-dyn, MF, and t_MF; cluster 2 was defined by an overrepresentation of MF; and, as a critical outcome, cluster 3 was defined by an overrepresentation of score-dyn and t_MF. Accordingly, participants belonging to cluster 3 had the highest score-dyn and t_MF. Our interpretative hypothesis is that internal interactions that adequately perform the task are reflected in a high degree of multifractal nonlinearity. These findings extend the notion that multifractal nonlinearity is a useful conceptual framework for shedding light on adaptive behavior during complex tasks. Full article

To enable intelligent inspection of underground cable systems, this study presents a gecko-inspired quadruped robot that integrates multi-degree-of-freedom motion with a deep learning-based visual detection system. Inspired by the gecko’s flexible spine and leg structure, the robot exhibits strong adaptability to confined and uneven tunnel environments. The motion system is modeled using the standard Denavit–Hartenberg (D–H) method, with both forward and inverse kinematics derived analytically. A zero-impact foot trajectory is employed to achieve stable gait planning. For defect detection, the robot incorporates a binocular vision module and an enhanced YOLOv8 framework. The key improvements include a lightweight feature fusion structure (SlimNeck), a multidimensional coordinate attention (MCA) mechanism, and a refined MPDIoU loss function, which collectively improve the detection accuracy of subtle defects such as insulation aging, micro-cracks, and surface contamination. A variety of data augmentation techniques—such as brightness adjustment, Gaussian noise, and occlusion simulation—are applied to enhance robustness under complex lighting and environmental conditions. The experimental results validate the effectiveness of the proposed system in both kinematic control and vision-based defect recognition. This work demonstrates the potential of integrating bio-inspired mechanical design with intelligent visual perception to support practical, efficient cable inspection in confined underground environments. Full article

To further enhance the economic performance of fuel cell vehicles (FCVs), this study develops a model-adaptive model predictive control (MPC) strategy. This strategy leverages the dynamic relationship between proton exchange membrane fuel cell (PEMFC) output characteristics and temperature to track its optimal operating temperature (OOT), addressing challenges of temperature control accuracy and high energy consumption in the PEMFC thermal management system (TMS). First, PEMFC and TMS models were developed and experimentally validated. Subsequently, the PEMFC power–temperature coupling curve was experimentally determined under multiple operating conditions to serve as the reference trajectory for TMS multi-objective optimization. For MPC controller design, the TMS model was linearized and discretized, yielding a predictive model adaptable to different load demands for stack temperature across the full operating range. A multi-constrained quadratic cost function was formulated, aiming to minimize the deviation of the PEMFC operating temperature from the OOT while accounting for TMS parasitic power consumption. Finally, simulations under Worldwide Harmonized Light Vehicles Test Cycle (WLTC) conditions evaluated the OOT tracking performance of both PID and MPC control strategies, as well as their impact on stack efficiency and TMS energy consumption at different ambient temperatures. The results indicate that, compared to PID control, MPC reduces temperature tracking error by 33%, decreases fan and pump speed fluctuations by over 24%, and lowers TMS energy consumption by 10%. These improvements enhance PEMFC operational stability and improve FCV energy efficiency. Full article

Background: Precision medicine refers to the formulation of personalized drug regimens according to the individual characteristics of patients to achieve optimal efficacy and minimize adverse reactions. Additive manufacturing (AM), also known as three-dimensional (3D) printing, has emerged as an optimal solution for precision drug delivery, enabling customizable and the fabrication of multifunctional structures with precise control over morphology and release behavior in pharmaceutics. However, the influence of 3D printing parameters on the printed tablets, especially regarding in vitro and in vivo performance, remains poorly understood, limiting the optimization of manufacturing processes for controlled-release profiles. Objective: To establish the fabrication process of 3D-printed controlled-release tablets via comprehensively understanding the printing parameters using fused deposition modeling (FDM) combined with hot-melt extrusion (HME) technologies. HPMC-AS/HPC-EF was used as the drug delivery matrix and carbamazepine (CBZ) was used as a model drug to investigate the in vitro drug delivery performance of the printed tablets. Methodology: Thermogravimetric analysis (TGA) was employed to assess the thermal compatibility of CBZ with HPMC-AS/HPC-EF excipients up to 230 °C, surpassing typical processing temperatures (160–200 °C). The formation of stable amorphous solid dispersions (ASDs) was validated using differential scanning calorimetry (DSC), hot-stage polarized light microscopy (PLM), and powder X-ray diffraction (PXRD). A 15-group full factorial design was then used to evaluate the effects of the fan speed (20–100%), platform temperature (40–80 °C), and printing speed (20–100 mm/s) on the tablet properties. Response surface modeling (RSM) with inverse square-root transformation was applied to analyze the dissolution kinetics, specifically t_50% (time for 50% drug release) and Q_4h (drug released at 4 h). Results: TGA confirmed the thermal compatibility of CBZ with HPMC-AS/HPC-EF, enabling stable ASD formation validated by DSC, PLM, and PXRD. The full factorial design revealed that printing speed was the dominant parameter governing dissolution behavior, with high speeds accelerating release and low speeds prolonging release through porosity-modulated diffusion control. RSM quadratic models showed optimal fits for t_50% (R² = 0.9936) and Q_4h (R² = 0.9019), highlighting the predictability of release kinetics via process parameter tuning. This work demonstrates the adaptability of polymer composite AM for tailoring drug release profiles, balancing mechanical integrity, release kinetics, and manufacturing scalability to advance multifunctional 3D-printed drug delivery devices in pharmaceutics. Full article

Fungi, from saprophytes to pathogens, face predictable daily fluctuations in light, temperature, humidity, and nutrient availability. To cope, they have evolved an internal circadian clock that confers a major adaptive advantage. This review critically synthesizes current knowledge on the molecular architecture and physiological relevance of fungal circadian systems, moving beyond the canonical Neurospora crassa model to explore the broader phylogenetic diversity of timekeeping mechanisms. We examine the core transcription-translation feedback loop (TTFL) centered on the FREQUENCY/WHITE COLLAR (FRQ/WCC) system and contrast it with divergent and non-canonical oscillators, including the metabolic rhythms of yeasts and the universally conserved peroxiredoxin (PRX) oxidation cycles. A central theme is the clock’s role in gating cellular defenses against oxidative, osmotic, and nutritional stress, enabling fungi to anticipate and withstand environmental insults through proactive regulation. We provide a detailed analysis of chrono-pathogenesis, where the circadian control of virulence factors aligns fungal attacks with windows of host vulnerability, with a focus on experimental evidence from pathogens like Botrytis cinerea, Fusarium oxysporum, and Magnaporthe oryzae. The review explores the downstream pathways—including transcriptional cascades, post-translational modifications, and epigenetic regulation—that translate temporal signals into physiological outputs such as developmental rhythms in conidiation and hyphal branching. Finally, we highlight critical knowledge gaps, particularly in understudied phyla like Basidiomycota, and discuss future research directions. This includes the exploration of novel clock architectures and the emerging, though speculative, hypothesis of “chrono-therapeutics”—interventions designed to disrupt fungal clocks—as a forward-looking concept for managing fungal infections. Full article

Objectives: Light intensity is a critical environmental factor regulating plant growth, development, and stress adaptation. However, the physiological and molecular mechanisms underlying light responses in Aconitum kusnezoffii, a valuable alpine medicinal plant, remain poorly understood. This study aimed to elucidate the adaptive strategies of A. kusnezoffii under different light intensities through integrated physiological and transcriptomic analyses. Methods: Two-year-old A. kusnezoffii plants were exposed to three controlled light regimes (790, 620, and 450 lx). Leaf anatomical traits were assessed via histological sectioning and microscopic imaging. Antioxidant enzyme activities (CAT, POD, and SOD), membrane lipid peroxidation (MDA content), osmoregulatory substances, and carbon metabolites were quantified using standard biochemical assays. Transcriptomic profiling was conducted using Illumina RNA-seq, with differentially expressed genes (DEGs) identified through DESeq2 and functionally annotated via GO and KEGG enrichment analyses. Results: Moderate light (620 lx) promoted optimal leaf structure by enhancing palisade tissue development and epidermal thickening, while reducing membrane lipid peroxidation. Antioxidant defense capacity was elevated through higher CAT, POD, and SOD activities, alongside increased accumulation of soluble proteins, sugars, and starch. Transcriptomic analysis revealed DEGs enriched in photosynthesis, monoterpenoid biosynthesis, hormone signaling, and glutathione metabolism pathways. Key positive regulators (PHY and HY5) were upregulated, whereas negative regulators (COP1 and PIFs) were suppressed, collectively facilitating chloroplast development and photomorphogenesis. Trend analysis indicated a “down–up” gene expression pattern, with early suppression of stress-responsive genes followed by activation of photosynthetic and metabolic processes. Conclusions: A. kusnezoffii employs a coordinated, multi-level adaptation strategy under moderate light (620 lx), integrating leaf structural optimization, enhanced antioxidant defense, and dynamic transcriptomic reprogramming to maintain energy balance, redox homeostasis, and photomorphogenic flexibility. These findings provide a theoretical foundation for optimizing artificial cultivation and light management of alpine medicinal plants. Full article

Avian influenza viruses (AIVs) pose significant risks to occupational populations engaged in poultry farming, livestock handling, and live poultry market operations due to frequent exposure to infected animals and contaminated environments. This review synthesizes evidence on AIV exposure patterns and risk factors through a comprehensive analysis of viral characteristics, host dynamics, environmental influences, and human behaviors. The main routes of transmission include direct animal contact, respiratory contact during slaughter/milking, and environmental contamination (aerosols, raw milk, shared equipment). Risks increase as the virus adapts between species, survives longer in cold/wet conditions, and spreads through wild bird migration (long-distance transmission) and live bird trade (local transmission). Recommended control measures include integrated animal–human–environment surveillance, stringent biosecurity measures, vaccination, and education. These findings underscore the urgent need for global ‘One Health’ collaboration to assess risk and implement preventive measures against potentially pandemic strains of influenza A viruses, especially in light of undetected mild/asymptomatic cases and incomplete knowledge of viral evolution. Full article

Manual assembly remains essential in modern manufacturing, yet the increasing complexity of customised production imposes significant cognitive burdens and error rates on workers. Existing Spatial Augmented Reality (SAR) systems often operate passively, lacking adaptive interaction, real-time feedback and a control system with gesture. In response, we present Gest-SAR, a SAR framework that integrates a custom MediaPipe-based gesture classification model to deliver adaptive light-guided pick-to-place assembly instructions and real-time error feedback within a closed-loop interaction instance. In a within-subject study, ten participants completed standardised Duplo-based assembly tasks using Gest-SAR, paper-based manuals, and tablet-based instructions; performance was evaluated via assembly cycle time, selection and placement error rates, cognitive workload assessed by NASA-TLX, and usability test by post-experimental questionnaires. Quantitative results demonstrate that Gest-SAR significantly reduces cycle times with an average of 3.95 min compared to Paper (Mean = 7.89 min, p < 0.01) and Tablet (Mean = 6.99 min, p < 0.01). It also achieved 7 times less average error rates while lowering perceived cognitive workload (p < 0.05 for mental demand) compared to conventional modalities. In total, 90% of the users agreed to prefer SAR over paper and tablet modalities. These outcomes indicate that natural hand-gesture interaction coupled with real-time visual feedback enhances both the efficiency and accuracy of manual assembly. By embedding AI-driven gesture recognition and AR projection into a human-centric assistance system, Gest-SAR advances the collaborative interplay between humans and machines, aligning with Industry 5.0 objectives of resilient, sustainable, and intelligent manufacturing. Full article

In the rapidly evolving field of biomedical engineering, hydrogels have emerged as highly versatile biomaterials that bridge biology and technology through their high water content, exceptional biocompatibility, and tunable mechanical properties. This review provides an integrated overview of both natural and synthetic hydrogels, examining their structural properties, fabrication methods, and broad biomedical applications, including drug delivery systems, tissue engineering, wound healing, and regenerative medicine. Natural hydrogels derived from sources such as alginate, gelatin, and chitosan are highlighted for their biodegradability and biocompatibility, though often limited by poor mechanical strength and batch variability. Conversely, synthetic hydrogels offer precise control over physical and chemical characteristics via advanced polymer chemistry, enabling customization for specific biomedical functions, yet may present challenges related to bioactivity and degradability. The review also explores intelligent hydrogel systems with stimuli-responsive and bioactive functionalities, emphasizing their role in next-generation healthcare solutions. In modern medicine, temperature-, pH-, enzyme-, light-, electric field-, magnetic field-, and glucose-responsive hydrogels are among the most promising “smart materials”. Their ability to respond to biological signals makes them uniquely suited for next-generation therapeutics, from responsive drug systems to adaptive tissue scaffolds. Key challenges such as scalability, clinical translation, and regulatory approval are discussed, underscoring the need for interdisciplinary collaboration and continued innovation. Overall, this review fosters a comprehensive understanding of hydrogel technologies and their transformative potential in enhancing patient care through advanced, adaptable, and responsive biomaterial systems. Full article