Search Results (100)

This contributed paper presents results from efforts by the Association of Zoos and Aquariums (AZA) to investigate the impact of integrating conservation into AZA members’ organizational cultures. Part of this work included AZA setting goals related to organizational and professional culture, strategic communication, developing communities of practice, and promoting tools and resource-sharing. Prior to implementing the majority of these steps, a baseline assessment was administered to directors plus a random sample of AZA organizations in 2020—assessing how well conservation is integrated into the institutional culture and measures of perceived conservation impact. The same sample of organizations was re-surveyed in 2023 with the intent of a three-year cycle of surveying to monitor change and identify additional ways that AZA could support and strengthen a culture of conservation within the profession. These findings will help the zoological and broader conservation community assess opportunities to integrate conservation into organizational cultures across a broad association for the purpose of achieving the mission and impact. Full article

Non-human primates are frequently used in anatomical and physiological research due to their similarity to humans. Currently, echocardiographic reference values are missing. Therefore, this study evaluated echocardiographic parameters in Callithrix spp., focusing on marmoset species under human care. Using color Doppler, M-mode, and B-mode, in a MyLab Gamma device (Esaote, Italy), examinations were conducted on 168 clinical healthy animals of the species Callithrix penicillata, C. aurita, C. jacchus, and hybrids, of both sexes, with an average weight of 328 ± 71 g. The samples were collected from the Mucky Project and the São Bernardo do Campo Zoo in São Paulo, Brazil, between November 2021 and May 2022. The animals were restrained with isoflurane, using a mask for induction and maintenance at a rate of 1 to 3% with 100% oxygen. Parameters such as the ventricular and atrial diameter, shortening fraction, and ejection fraction, among others, were measured. Cardiac reference values were assessed. The mild insufficiencies of the mitral and tricuspid valves were the most common findings in cases with alterations. These data serve as a refence for monitoring cardiac health in marmosets, aiding in both conservation and the management of captive primates. Full article

Zoos and aquariums house a wide range of species, yet research remains heavily skewed toward mammals and camera-based technologies. This systematic literature review examines the use of computing technologies to monitor or interact with animals in zoos, aquariums, or wildlife parks, with a focus on taxonomic representation and technological diversity. A total of 125 studies published between 2014 and 2024 met the inclusion criteria, encompassing 151 recorded instances of technology use. Cameras and video systems were the most frequently used tools, appearing alone in 40% of studies and in combination with other technologies in an additional 18.4%, accounting for 48.3% of all technology instances. Most studies focused on only mammals (73.5%), and behavioral monitoring was the most common research aim (40.9%). These findings suggest an uneven distribution of research shaped more by convenience and familiarity than by welfare need, highlighting a critical opportunity to diversify both species focus and technological application. Increased investment in underrepresented species and underutilized tools will help ensure that research better reflects the full spectrum of animal needs and experiences. Full article

This study evaluates the health of a captive colony of Hamadryas baboons at Ravenna Zoo Safari (Italy), focusing on oxidative stress markers and biometric data. Forty-eight individuals were assessed during routine veterinary procedures: males underwent vasectomy, and females were checked for pregnancy. Biometric data collected included body weight, body length, and genital measurements in males, while females were evaluated for reproductive status. Oxidative stress was measured using two tests that assess both harmful pro-oxidant levels and the body’s antioxidant defenses. Results showed no significant differences in oxidative stress levels between sexes, although males and females differed in body weight. Pregnant and postpartum females exhibited higher oxidative stress, likely due to the metabolic and hormonal demands of reproduction. This supports the idea that reproductive activity increases the production of reactive oxygen species, requiring stronger antioxidant responses. In males, correlations between body weight and genital measurements suggest these could help estimate age in the absence of birth records. No link was found between oxidative stress and body weight, indicating limited age-related effects on these markers. Overall, the study highlights the importance of monitoring oxidative stress in captive primates to better understand the effects of reproduction and aging, and to improve welfare and management practices. Full article

The tree monitor lizards of the subgenus Hapturosaurus are an understudied but increasingly important group of reptiles in zoos. The routine blood analysis of reptiles in captivity is highly recommended; however, the lack of reliable reference values for many species severely limits their clinical use. This study aims to establish preliminary reference values (RV) for the main hematological and biochemical parameters in three species of the subgenus Hapturosaurus (Varanus macraei, Varanus prasinus and Varanus beccarii). Blood samples were taken from healthy adult individuals (n = 34), females (n = 18) and males (n = 16), maintained under controlled conditions in a zoo. A complete biochemical analysis was performed on all individuals, including alanine-aminotransferase, alkaline phosphatase, gamma-glutamyl transpeptidase, aspartate-aminotransferase, creatine kinase, bile acids, urea, uric acid, total solids, total proteins, albumin, glucose, fructosamine, lactate, cholesterol, triglycerides, amylase, calcium, phosphorus, calcium:phosphorus ratio, and magnesium. In addition, a complete hematological analysis was conducted on these individuals, including hematocrit, hemoglobin concentration, total erythrocyte and leukocyte counts, and leukocyte differential count. From the data obtained, reference intervals were established following the guidelines provided by the American Society for Veterinary Clinical Pathology (ASVCP). In this study, the results of the three species were unified to increase the sample size and to obtain more representative reference intervals. This unification was supported by a statistical analysis using the one-factor ANOVA test, which showed no statistically significant differences between species (p < 0.050). These data constitute an essential first step for the clinical practice and management of these Hapturosaurus species in zoos and specialized centers. Future collaboration with other institutions will help expand the dataset and enhance the accuracy of the established reference values. Full article

Background/Objectives: This study aims to validate the tests in the VIRTRAEL assessment module for individuals in the chronic stage of Acquired Brain Injury (ABI). Methods: A total of 32 participants (81.3% male) were assessed, including 20 individuals who had experienced a stroke (62.5%) and 12 who had experienced a traumatic brain injury (TBI). Cognitive functions such as attention (d2 Attention Test and Digit Subscale of the WAIS-III), learning-memory (HVLT-R and Letters and Numbers Subscale of the WAIS-III), and executive function (Similarities and Matrices Subscales of the WAIS-III and the Zoo Map Test, version 1 of the BADS Battery) were evaluated using standardized paper-and-pencil tests and computerized tests from the VIRTRAEL online platform. Convergent and divergent correlation analyses were conducted using SPSS version 28. Results: Significant correlations were observed between scores in both formats (ranging from r = 0.408 in planning to r = 0.818 in delayed verbal recall), except in reasoning and verbal memory recognition. Conclusions: The tests from the free online platform VIRTRAEL demonstrate adequate validity for the cognitive assessment of individuals with ABI. This suggests that VIRTRAEL is a valuable, accessible tool for monitoring cognitive status, potentially benefiting health, education, and social and occupational reintegration. Full article

Infections with the zoonotic pathogen Yersinia (Y.) pseudotuberculosis are commonly causing clinical diseases and acute deaths in various mammal and bird species in zoos. These findings prompted us to conduct a targeted study that included animals of 18 German and 1 Swiss zoo that had been affected by the pathogen previously. This study provides a comprehensive overview of susceptible zoo animal species, clinical signs, therapies, antimicrobial resistance, pathogen monitoring, and prophylactic measures. In addition, fecal samples from species with documented infections and organ samples from deceased mammals and birds from two of the participating zoos, the urban zoo Wilhelma and the rural Opel Zoo, were examined for Y. pseudotuberculosis using both direct plating and enrichment. The overall prevalence of Y. pseudotuberculosis was 3.1% at the Opel Zoo and 1.3% at the Wilhelma. Fecal samples yielded positive results in 1.4% of the tested samples from the Opel Zoo but none from the Wilhelma. Among carcasses, 16.7% and 1.7% tested positive at the Opel Zoo and the Wilhelma, respectively (p = 0.006). Y. pseudotuberculosis was significantly more frequently isolated during the cold season (p = 0.002). Affected animals often died suddenly, displaying no or only non-specific clinical signs, but postmortem examinations revealed septicemia with multiple bacterial organ abscesses. Rodents, ruminants, primates, and Piciformes were the most commonly affected orders. Considering the zoonotic potential of Y. pseudotuberculosis, this research underscores the importance of investigations in zoos in accordance with the targets of the One Health approach. Full article

Zoos and botanical gardens have a special role in the promotion, presentation and conservation of biodiversity in urbanised environments. Additionally, they provide special habitats for alien and invasive species. The formerly used methods of biodiversity assessments (e.g., light trapping, transect counts, etc.) are mostly labour-intensive and/or not efficient enough. In the Zoo of Debrecen, the efficacy and suitability of a synthetic (FLO) and a semisynthetic (SBL) lure for this purpose were proven. The qualitative and quantitative compositions of a moderately rich moth assemblage including 52 moth species were revealed, and the appearance and population dynamics of three invasive (Helicoverpa armigera, Autographa gamma and Cydalima pespectalis) and nine harmful pest species were also recorded. The results proved that the lures tested and traps used provide an easy-to-use, standardised and relatively cheap method for Zoos and botanical gardens to assess their biodiversity even in the case of limited resources. Full article

Tropical forest hornbills display complex social and reproductive behaviours that make them hard to reliably and frequently breed in ex situ facilities. This study investigated potential behavioural indicators of pair compatibility for two wreathed hornbills (Rhyticeros undulatus) at ARTIS Amsterdam Royal Zoo. We focused on behaviours linked to reproductive investment, such as time spent in proximity, vocalisation, and courtship display. Through systematic observations using ZooMonitor, we recorded behavioural and enclosure use data, as well as visitor presence. Key findings revealed that the male hornbill was more active and exploratory than the female. Courtship behaviours were more frequently performed by the male bird and were positively associated with perching at elevated positions. However, this behaviour decreased when the birds were in close proximity or inactive. Visitor presence influenced enclosure use, with the hornbills preferring higher perches during peak visitor times. We conclude that behaviours such as proximity, vocalisations, and courtship displays could potentially serve as indicators of compatibility in hornbill pairs but more data across more birds are needed for validation. Finally, we advocate for enclosure designs that consider hornbills’ natural behaviours and social needs to help ex situ facilities promote an environment conducive to pair bonding. Full article

Several species of amoebae in the genus Entamoeba forming four-nucleated mature cysts are pathogenic, but their identification is challenging due to a lack of distinctive morphological traits. Furthermore, host species cannot reliably be used as a criterion for identification. In this study, several isolates of four-nucleated cyst-forming Entamoeba species from animals at the Zoo Aquarium of Madrid are genetically identified. Entamoeba cysts were identified through microscopic analysis of faecal samples; none of the animals exhibited symptoms. DNA was extracted, and a fragment of the SSU-rRNA gene was PCR-amplified and sequenced to identify the species. Entamoeba dispar was identified in mantled guerezas (Colobus guereza), lar gibbons (Hylobates lar), greater rheas (Rhea americana) and giant anteaters (Myrmecophaga tridactyla). Entamoeba hartmnanni was detected in mandrills (Mandrillus sphinx) and chimpanzees (Pan troglodytes). Entamoeba nuttalli was detected in mandrills; the analysis of sequences available in GenBank revealed that this species has also been detected in human samples. The identification of the Entamoeba species across diverse host species underscores the need for genetic confirmation to prevent misidentifications. These findings expand the known host range of these species and emphasize the importance of regular monitoring to minimize transmission risks, including potential cross-species transmission to/from humans. Full article

This study examined the ecological status of the Streževo Reservoir in North Macedonia, focusing on zooplankton as an indicator of water quality. Built in 1982, the Streževo Reservoir serves several purposes, including irrigation, water supply, and hydropower generation. The research project investigated the seasonal and vertical variation in zooplankton abundance and biomass as well as the influence of environmental factors. Sampling was conducted seasonally (spring, summer, and autumn) in 2010 and 2011 across the longitudinal profile (epilimnion, metalimnion, and hypolimnion) of the reservoir at three sampling stations: the inflow of the Šemnica River, a central station in open water, and a site near the dam. The Streževo Reservoir is characterized by significantly pronounced seasonal and vertical temperature stratifications. The species diversity of the zooplankton was low, with only 21 taxa identified. Seasonal oscillations in abundance were statistically significant, with maximum values in the summer period and minimum values in spring. The Shannon diversity index displayed the lowest diversity values in the autumn, in the hypolimnion, and the highest values in the summer, in the metalimnion. The RDA analysis showed that temperature was the most important predictor of zooplankton abundance distribution, followed by Chl a concentration and TN. According to the Zooplankton Index of Quality Assessment (Zoo-IQ), during the investigated period the reservoir had good water quality in all three studied seasons, as well as through the whole profile. Overall, the study highlights the importance of zooplankton as an indicator of water quality and provides valuable insights into the ecological status of the Streževo Reservoir. The novelty of this study lies in its comprehensive examination of the interconnected dynamics affecting reservoir ecology, particularly as the present study is the first to perform such an analysis for the Streževo Reservoir. It highlights the impacts of thermal stratification on biochemical processes, the seasonal variations in dissolved oxygen and phosphorus levels due to phytoplankton activity, and the influences of temperature on zooplankton diversity and abundance. Furthermore, it introduces the Zoo-IQ index as an innovative tool for assessing water quality through zooplankton analysis, emphasizing its relevance as an early indicator of ecological changes in freshwater systems. Moreover, this multi-faceted approach underscores the complexity of reservoir ecosystems and the importance of proactive management strategies to the mitigation of water quality fluctuations. This study underlines the need for continuous monitoring and proactive management strategies to address the aging of reservoirs. Full article

In captive environments, mammals are frequently exposed to various parasitic protozoa and other vector-borne pathogens that can impact both animal health and public health. Monitoring these pathogens is essential for animal welfare and zoonotic disease control. This study aimed to investigate the prevalence of parasitic protozoa and other vector-borne pathogens in captive mammals through molecular detection methods at the Belo Horizonte Zoo, Brazil. Between November 2021 and March 2023, whole blood samples were collected from 40 mammals. Molecular analyses identified piroplasms, Leishmania spp., granulocytic/platelet Anaplasma/Ehrlichia spp., monocytic Ehrlichia spp., Bartonella spp. and hemotropic Mycoplasma spp. with a 72.5% positivity rate. Piroplasms were found in 22.5% (two Pantanal cats, two gorillas, one white rhinoceros, one spider monkey, one jaguar, one tufted capuchin and one hippo) and Leishmania spp. in 12.9% (four maned wolves). Granulocytic/platelet Anaplasma/Ehrlichia spp. were found in 12.5% of the samples (one gorilla and four maned wolves), Ehrlichia canis in 2.5% of the animals (one maned wolf), Bartonella spp. in 42.5% (six howler monkeys, two maned wolves, one gorilla, one white rhino, one southern tamandua, one common woolly monkey, one tufted capuchin, one brown brocket deer, one agouti, one cougar and one hippo), hemotropic Mycoplasma spp. in 17.5% (one gorilla, one maned wolf, one white rhino, one howler monkey, two common woolly monkeys and one European fallow deer). Five Artiodactyla members tested negative for A. marginale. Coinfections occurred in 34.5% of the positive samples. Sequencing revealed that Theileria spp. and Cytauxzoon spp. are closely related to Theileria bicornis and Cytauxzoon felis; Ehrlichia canis and Bartonella spp. are closely related to B. clarridgeiae and B. henselae; and hemotropic Mycoplasma spp. are closely related to Candidatus Mycoplasma haemominutum. Our results showed a high occurrence of vector-borne pathogens in captive animals, including zoonotic species, which may pose a risk to animal and human public health. Full article

Humans have long delighted in being entertained by captive wild animals. In zoos and aquariums, animal–visitor interactions allow visitors to experience direct or indirect contact with animals. Well-managed animal–visitor interactions can help zoos and aquariums contribute to their educational objectives by eliciting pro-conservation behaviours from visitors while providing good animal welfare for the animals involved. However, some interactions are dysfunctional because they cause physical and psychological harm to the animal and reinforce negative values and beliefs towards conservation. We aimed to assess how dysfunctional animal–visitor interactions are and whether accreditation by zoological associations reduced the number of such interactions. We visited Thai zoos in person and conducted online monitoring of facilities in Southeast Asia. We report 1165 animal–visitor interactions in nine Southeast Asian countries in 225 zoos and aquariums. Taking photos with animals (“selfies”) and riding on animals were scored as the animal–visitor interactions that were the most dysfunctional. The number of animal–visitor interactions was not less in accredited zoos, and there was little difference among countries. Behaviour change needs to form a strategy for change, in addition to robust legislation and improved efforts by zoological associations to push members to maintain standards and mentor non-accredited facilities. Full article

Strategically enriching the environment of zoo felids to encourage innate behaviors is essential to good welfare. To investigate how engagement differs among sensory-based enrichment, six items representing tactile (firehose log, Jolly Eggs), visual (mirror, movie), and olfactory (cinnamon, cologne) sensory categories were offered to three adult cheetah siblings. Each item was offered four times: twice during daytime hours and twice overnight (≥5 h/occasion). Static video monitoring was used to investigate engagement frequency and duration for each item and to observe grooming behaviors. Cheetahs engaged with visual enrichment more frequently (p < 0.05) and for greater (p < 0.05) periods than other items. Allogrooming occurred most frequently (p < 0.05) when the movie was provisioned at night. Tactile enrichment was only modestly engaged, with fewer but longer engagements (p < 0.05) at nighttime than at daytime. Autogrooming frequency did not vary among enrichment items but was greater (p < 0.05) at night than day. These findings showed that zoo cheetah responses to sensory enrichment items differed based on type and time of day. Visual stimuli were favored, whereas olfactory stimuli were generally ignored. This illustrates the benefit of diverse sensory-specific enrichment strategies. Such dynamic strategies can help overcome the challenge of individual preferences to encourage engagement reflective of the animal’s natural behaviors. Full article

Unusual or extraordinary circumstances can cause change to normal husbandry regimes and daily care of managed animals. Increased biosecurity due to disease risk, for example, results in animals experiencing restrictions. Outbreaks of Highly Pathogenic Avian Influenza (HPAI) have caused zoos to remove birds from their regular exhibits and manage them indoors or in covered enclosures to reduce the likelihood of an HPAI outbreak on site. To date, there has been little research on the impacts of such husbandry change on bird behaviour and welfare. This paper examines the effect of an HPAI-induced enforced housing order (HO) on the behaviour and physical condition of a flock of Chilean flamingos in a UK zoo. Using ZooMonitor to record flock-wide behaviour patterns and scoring plumage condition, we collected data on flamingos during a housing order, immediately after lifting of the HO, and after a period of acclimation to their regular routine. Results showed that flamingos were very inactive under a HO and after release, that abnormal, redirected foraging actions occurred during the HO and after release, and that flamingos were more alert under the HO. An increase in records of good plumage condition correlated with social behaviour, inactivity, higher temperatures, and rain. This research highlights the multifactorial influences on zoo animal behaviour and shows why information on the animals, their inputs, the behavioural outputs they present, and their physical attributes should all be gathered and evaluated together to best understand the influences of husbandry and management changes on behaviour and welfare. Full article