Search Results (12)

Understanding how chemical composition, phenolic profile, volatile compounds and sensory attributes evolve during wine aging is essential for optimizing quality in premium red wines. This study investigated four consecutive vintages (2017–2020) of Xinomavro red wine (PDO Amyndeo), each produced under identical viticultural and enological conditions and aged for either 12 or 24 months in French oak barrels. Comprehensive chemical, phenolic, colorimetric and volatile analyses were combined with sensory evaluation and multivariate statistics to elucidate vintage- and aging-driven differences. Wines aged for 24 months exhibited higher phenolic richness and antioxidant capacity, with the 2020–24 m sample showing the highest total polyphenols (4230 mg GAE/L) and FRAP values. Sensory analysis revealed clear differentiation among vintages, with younger wines expressing red fruit and tomato leaf aromas, while older vintages displayed dried fruit, caramel, spice and oak-derived notes. PCA demonstrated that PC1 captured phenolic and antioxidant variation, PC2 reflected chromatic intensity and residual sugars, and PC3 represented spicy/vegetal and acidity-related attributes. Multivariate correlation analysis confirmed strong associations between phenolic indices, volatile compounds, and sensory descriptors. Overall, the integration of chemical fingerprinting, volatile profiling and sensory evaluation provides valuable insights into how vintage and barrel aging shape the complexity and quality of Xinomavro wines. Full article

The sustainability of the wine sector depends on primary production practices and on the adaptability of plant material to climate change. This study evaluates the carbon footprint and technical efficiency of four grape varieties in Paionia using an integrated Life Cycle Assessment and Data Envelopment Analysis framework. A cradle-to-gate approach was adopted, with system boundaries extending from input production to harvest, and functional units of kg CO₂e/ha to capture input intensity and kg CO₂e/kg grape to assess product-level environmental efficiency. The analysis included 82 vineyards, with DEA scores ranging from 0.744 to 1.000; most vineyards operated below the efficiency frontier, and the input-oriented VRS model identified potential input reductions without affecting output. Merlot showed the highest footprint (3794.02 kg CO₂e/ha), followed by Assyrtiko (2798.40) and Xinomavro (2784.48), while Roditis had the lowest (1958.07); on a per-kg basis, emissions were 0.340, 0.304, 0.281, and 0.143 kg CO₂e/kg respectively. The DEA identified targeted input-saving opportunities, including reduced irrigation needs in white varieties and lower nutrient and plant-protection requirements in red varieties, while the strong performance of Roditis highlights the advantages of locally adapted, low-input plant material for improving efficiency. Full article

This study focuses on identifying wine-growing subzones within the PDO Amyndeon and PGI Drama wine-growing zones in Northern Greece, with the aim of assessing their suitability for producing high-quality red wines from the Xinomavro (Vitis vinifera L.) and Cabernet Sauvignon (Vitis vinifera L.) grape varieties, respectively. The initial delineation of suitability zones was carried out using readily available satellite data on soil, topography, and climate, in four different suitability categories. To validate how effectively these categories distinguished actual wine-growing regions, we compared them against two years of field data collected from experimental vineyards. The results showed that this methodology was able to discern the most suitable areas for both varieties and regions with an acceptable relation to real grape and wine attributes as confirmed by the collection of data from the pilot vineyards. The overall performance of this method will ultimately depend on the validity of the expert knowledge used to define the most critical parameters and their range. According to the results of this study, and given the relevance of the proposed suitability criteria, this method has the potential to provide an alternative solution for subzone delineation in cases where wine analytical and sensory data are not available. Full article

Wine samples (72) of different types (white, rose and red), dry, originating from different regions of Greece (Northern Greece, Central Greece, Peloponnese, Aegean Islands, and Crete), were analyzed for Ochratoxin A (OTA) presence. Wine samples, originating from Greek (Assyrtiko and Xinomavro) and international (Syrah and Sauvignon blanc) noble grapevine varieties vintaged from 2020 to 2023, were analyzed using a modified QuEChERS extraction protocol followed by HPLC with a fluorescence detector to detect and quantify OTA. Moreover, conventional oenological parameters were measured according to OIV official methods, and climatic conditions of the regions of concern were retrieved. Interestingly, in general, OTA contaminated wines showed low concentrations (<2.0 μg/L). The highest concentrations of OTA were detected in Sauvignon blanc (7.5 μg/L) regarding the white wines and Xinomavro (2.07 μg/L) regarding the red ones. In addition, the highest OTA concentrations were recorded in wines produced in areas either with high mean annual temperatures, like Viotia (24.16 °C) for white and Larissa (23.9 °C) for red wines, or with high rainfall between May and September (Larissa 69.76 mm) for white wines. Consequently, it was observed that concentrations of OTA in wine are relatively higher in the warmer regions of Greece compared to the cooler areas. The effect of climate change on vines and mycotoxin presence in wine needs urgent consideration by well-constructed modelling studies and efficient interpretation of existing data. The evaluation of OTA presence in grape products originating from various cultivars and regions is imperative not only for providing crucial data to predict its fate under climate change, but also to ascertain the potential risk of human exposure to this chemical compound. Full article

‘Xinomavro’ (V. vinifera L.) is an important native red wine grape variety in Northern Greece, particularly in PDO (protected designation of origin) regions. Despite its significance, there is limited research on the effects of pruning type and severity on ‘Xinomavro’ vine physiology, yield, and berry quality across diverse environmental conditions. This study aimed to address this knowledge gap and provide growers with crucial information for optimizing vineyard management practices. The study was conducted over two consecutive years (2016 and 2017) in a vineyard in Thessaloniki, Northern Greece. Four treatments (B12: 12 buds on 6 spurs, B24: 24 buds on 12 spurs, M12: 12 buds on 2 canes, and M24: 24 buds on 4 canes) combining two bud load levels (12 or 24 count nodes) and two pruning types (short spurs or long canes) were applied to ‘Xinomavro’ vines in a complete block randomized design. The vine water status, gas exchange, canopy characteristics, yield components, and berry composition were measured. Bud load and pruning type significantly influenced vine canopy development, microclimate, and yield components. Short pruning with high bud load (B24) resulted in denser canopies and higher yields, whereas cane pruning (M12 and M24) led to more open canopies and improved berry quality indicators. Treatment effects on berry composition were inconsistent across years but showed a tendency for higher anthocyanin and total phenol content in cane-pruned vines. This study demonstrates that pruning type (short or long fruiting units) may have a greater impact on vine growth, yield, and berry composition than bud load alone in ‘Xinomavro’ vines. Cane pruning appears to be a more effective strategy for achieving vine balance and potentially improving grape quality under given experimental conditions. Full article

A three-year study was conducted to investigate the effects of two timings—at berry set and at veraison—of post-bloom leaf removal (LR) applications from the basal sections of the shoot on the growth, yield, and berry composition of Vitis vinifera L. cv. Xinomavro, the major red winegrape of North Greece. LR at berry set drastically reduced yield by affecting all its components while increasing the ratio of secondary foliage per total leaf area. LR at veraison had a milder effect on growth and yield. Both treatments increased sugar and phenolic compound levels in berries, while early LR increased the contribution of more stable forms of anthocyanins. Additionally, early LR increased the skin-to-pulp ratio, suggesting that it could be an effective viticultural technique for more concentrated red wines. Overall, both LR timings improved most of the berry attributes compared to the non-treated vines; however, the earlier application can be recommended when aiming at the production of deep-colored and richly flavored wines. Full article

This study explored the potential of pulsed electric field (PEF) as an alternative wine-aging method in four Xinomavro red wines with the implementation of several wood chips (apricot, peach, apple, cherry, acacia, and oak trees). The evolution of total polyphenol content (TPC) and sensory properties of the wines were investigated. Sensory evaluation revealed that PEF treatment increased volatile compound extraction from each wood chip, thereby enhancing the overall quality of the wines. The utilization of acacia tree wood chips in Goumenissa wine led to a notable increase of 10.84% in TPC from the control sample, reaching 2334.74 mg gallic acid equivalents/L. A notable outcome was that PEF decreased TPC, a trend that was also verified through correlation analyses. The highest positive impact of PEF was observed in peach tree wood chips in Goumenissa wine, with a significant increase of 11.05% in TPC. The results from the volatile compound analysis revealed an increase in alcohols and esters from 0.24% to 23.82%, with the highest proportion found in 2-phenylethanol (16.92 mg/L) when utilizing peach tree wood chips in the production of Amyndeo wine. This study could provide a benchmark for rapid, efficient, and cost-effective wine aging through the implementation of the PEF process. Full article

Pulsed electric field (PEF) treatment has gained significant attention within the food industry. This study examines the application of PEF combined with wood chips of diverse species to expedite the flavor aging process of Xinomavro red wine. Various wood chip species, including black locust, common juniper, apricot, sweet chestnut, cherry, apple, peach, and European oak, sourced from pruning residues were immersed in the wine prior to subjecting it to PEF treatment. The samples underwent a range of pulse durations and intervals during treatment. Comparative preparations encompassing wine without chips and wine infused with each wood type left at ambient temperature for 5 days were also examined. The sensory attributes and the volatile compounds (VC) were assessed through the utilization of headspace solid-phase microextraction and GC-MS. In the control sample, 12 VCs were identified, whereas in the samples, 22 distinct VCs were identified. Favorable sensory attributes across all PEF conditions were associated with the incorporation of cherry wood chips. These findings highlight the potential of PEF treatment to enhance the quality parameters of the aging process in Xinomavro red wine, capitalizing on the synergistic interaction between PEF and various wood chip species. This innovative approach holds promise for augmenting crucial oenological parameters of red wine, strengthening the use of PEF as an efficient technique to enhance the overall quality. Full article

Despite Xinomavro (Vitis vinifera L.) being a well-known noble red grape variety of northern Greece, little is known about its ‘‘bouquet’’ typicity. Volatile compounds of Xinomavro wines produced using a common vinification protocol were analyzed by gas chromatography–mass spectrometry and sensory descriptive analysis was carried out with a trained panel. Wines were characterized by the presence of fatty acids, ethyl and acetate esters, and alcohols, with contributions from terpenes and a volatile phenol. The most active aroma compounds were determined to be 3-methylbutyl acetate, β-damascenone, ethyl esters of octanoic and hexanoic acids, and eugenol. Those compounds positively correlated with fruity and spicy odor descriptors, with the wines being mostly characterized by five typical aroma terms: strawberry, berry fruit, spices, tomato, and green bell pepper. Partial least squares regression (PLSR) analysis was used to visualize relationship between the orthonasal sensory attributes and the volatile aroma compounds with calculated OAVs > 1. Key aroma-active volatiles in the wines were identified using GC-MS/olfactometry, providing a list of 40 compounds, among which 13 presented a modified detection frequency > 70%. This study is the first of its kind and provided strong indications regarding the aroma compounds defining the sensory characteristics of Xinomavro wines. Full article

Wine and by-products of the winemaking process, such as grape stems, are rich in bioactive polyphenolic compounds that might be beneficial for animal and human health. In recent years, the administration of dietary polyphenols with strong antioxidant and cytoprotective properties has constituted an emerging line of research interest toward disease prevention. However, in scientific literature, only a limited number of studies have investigated the safety and the toxicological risks of polyphenolic compounds in vivo. Based on the above, the purpose of the present study was two-fold: first, to examine the effects of oral administration of a grape stem extract, derived from the Greek red wine Mavrodaphne, on mice redox biomarkers; and second, to investigate the biological effects of oral administration of a wine extract, derived from the emblematic Greek red wine Xinomavro, on rats. Toward this purpose, body weight, growth rate, hematological, biochemical, and histopathological parameters, as well as a panel of redox biomarkers, were examined. According to our results, the administration of Mavrodaphne grape stem extract in mice induced alterations in redox homeostasis, preventing mice from the adverse effects of lipid peroxidation. Contrariwise, the administration of Xinomavro wine extract induced both beneficial and harmful outcomes on rat redox status determined by the examined tissue. Collectively, our study reports that the Mavrodaphne grape stem extract, a serious pollutant when disposed in environmental matrices, is an important source of bioactive polyphenolic compounds that could protect from oxidative damage and improve animal and human health. Finally, the Xinomavro wine extract exerts tissue-specific changes in redox balance, which are indicative of the complexity that characterizes the biological systems. Full article

Wine is an alcoholic beverage of complex composition obtained through the fermentation of grape must. The consumption of wine has already been associated with a multitude of beneficial effects due to its high polyphenolic content. In this study, four Greek emblematic wines from two red (i.e., Xinomavro and Agiorgitiko) and two white (i.e., Assyrtiko and Malagouzia) varieties were analyzed for the estimation of their antioxidant profiles. To address this question, we assessed their ability to scavenge both synthetic and endogenous free radicals, such as DPPH^•, ABTS^+•, OH^•, O₂⁻, their potential reducing power, and their antimutagenic and antigenotoxic properties. All varieties exhibited potent antioxidant activity, as indicated by the results of methods above, with the red wines appearing more effective than the white ones regarding antioxidant capacity. Our small-scale study is the first to reveal that these wine varieties may have the ability to scavenge the most reactive endogenous radicals. In the future, this finding must be accompanied by larger studies to fill a knowledge gap in the scientific literature concerning a holistic approach of the in vitro antioxidant action of plant polyphenolic compounds. Conclusively, we believe that wines possess high bioactivity that allow them to settle in the industry of food additives and medicinal products. Full article

Wine metabolomics constitutes a powerful discipline towards wine authenticity assessment through the simultaneous exploration of multiple classes of compounds in the wine matrix. Over the last decades, wines from autochthonous Greek grape varieties have become increasingly popular among wine connoisseurs, attracting great interest for their authentication and chemical characterization. In this work, 46 red wine samples from Agiorgitiko and Xinomavro grape varieties were collected from wineries in two important winemaking regions of Greece during two consecutive vintages and analyzed using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QToF-MS). A targeted metabolomics methodology was developed, including the determination and quantification of 28 phenolic compounds from different classes (hydroxycinnamic acids, hydroxybenzoic acids, stilbenes and flavonoids). Moreover, 86 compounds were detected and tentatively identified via a robust suspect screening workflow using an in-house database of 420 wine related compounds. Supervised chemometric techniques were employed to build an accurate and robust model to discriminate between two varieties. Full article