Search Results (21,422)

In classical reliability engineering, failure is a probabilistic structural failure based on lifetime distributions of Weibull models. However, in the control-critical mechanical systems, it is possible that functional failure of the system happens before material failure occurs as a result of control power loss. This paper proposes a Controllability–Reliability Coupling (CRC) model, which redefines the concept of reliability as the stabilizability in the face of progressive degradation. The actuators’ deterioration is modeled using the time-varying input effectiveness factor $\alpha \left(t\right)$, and the actuator is said to be in failure when the minimum singular value of the finite-horizon controllability Gramian becomes less than a stabilizability threshold $\epsilon$. The performance of the simulation indicates that the functional failure is a precursor of structural failure in several degradation conditions. A baseline comparison shows that the CRC metric forecasts loss of controllability at $T_{CRC}=17.0$ s, but the classical Weibull reliability never attains the structural failure threshold even in the time horizon of 20 s. The system retains margins of Lyapunov stability and H infinity robustness are not lost, and it is still stable and attenuates disturbances even when control authority is lost. In practical degradation scenarios, the forecasted CRC failure times are 21.5 s (linear wear), 13.1 s (accelerated fatigue), 23.7 s (intermittent faults), and 24.4 s (shock damage), whereas maintenance recovery abated functional failure completely. In a case study of an industrial robotic joint, at 27.0 s, functional collapse occurred, and at the same time, structural reliability was still above the failure threshold. The findings support the hypothesis that structural survival and functional controllability are distinct concepts. The proposed CRC framework is an approach to control-conscious reliability measure, which can detect early failures and offer proactive maintenance advice in the context of a cyber–physical system. Full article

It is shown that transient trapped surfaces form in a class of emerging globally hyperbolic spacetimes, within punctured Planck-scale neighbourhoods of the geon supported on intersecting singular supports whose intersection forms a characteristic core in a non-strongly causal setting. These neighbourhoods shrink towards the intersecting singular support in the distributional geometry. In particular, the trapped surfaces occur near the characteristic limit corresponding to the unstable equilibrium of the self-gravitating geon. They act as an effective classical barrier for descriptions formulated purely within smooth differential geometry. The area of these trapped-surface configurations, computed on Planck-referenced neighbourhoods, is shown to tend to zero both in the asymptotically flat limit of the emerging spacetime and in the geon limit. Thus, transient trapped surfaces evaporate in the sense that their area vanishes as classical and asymptotically flat spacetime emerges within the quantum foam framework. A state-counting generating function for the transient trapped surfaces is constructed from the coherent-state density operator. This generating function maps microscopic occupation-number sectors to macroscopic data and thereby allows a definition of Boltzmann entropy (not to be confused with the von Neumann entropy, which is zero for any pure coherent state). Since the coherent state is constructed to implement the correspondence principle, expectation values of the relevant quantised observables reproduce their classical values. In particular, the expectation value of the bosonic occupation-number operator serves as a microstate-counting variable in the coherent sector. The generating function takes the form of an exponential of this expectation value, leading to an entropy–area relation consistent with the Hawking–Bekenstein scaling. Full article

This study explores the feasibility of using municipal solid waste incineration bottom ashes (MSWIBAs) as a partial replacement for cement in concrete with respect to the fresh and hardened properties of concrete. MSWIBA samples from five Swedish incineration plants (BA1–BA5) were collected and analyzed for their mineral composition and particle size distribution (PSD). The samples (BA3 and BA5), exhibiting better pozzolanic behavior and particle sizes closer to those of conventional cement, were selected for further detailed study. Mechanical activation was performed on the BA3 and BA5 samples. Concrete mixes were prepared with 10% and 20% (by mass) cement replacements utilizing raw and activated BA3 and BA5 samples. The resulting concrete specimens were evaluated through slump, density, and compressive strength tests at 7, 28, and 56 days. The results showed that activated MSWIBAs improved the workability of the concrete specimens compared with the control concrete mix, and the density of the concrete decreased with increasing the MSWIBA content. The compressive strength of the concrete mixes generally decreased as the replacement level of MSWIBAs increased. At 56 days, the concrete mix with 10% raw BA5 reached about 77% of the compressive strength of the control concrete mix, whereas mixes with 20% raw or activated MSWIBAs reached about 58%. The concrete mix with BA3 performed better than the mix with BA5 at 7 days, while the concrete mix with BA5 showed higher later-age compressive strength. In addition, mechanical activation of MSWIBAs did not significantly improve compressive strength of concrete mixes. Despite the reduction in compressive strength when using MSWIBAs, this sustainable concrete contributes to the development of climate-friendly concrete and offers potential environmental benefits. Full article

Colored potato cultivars are rich in phenolic compounds that confer high antioxidant capacity; however, these beneficial metabolites could be susceptible to oxidation by polyphenol oxidases (PPOs), leading to enzymatic browning and the loss of antioxidant potential. Despite the agronomic relevance of this trade-off, the dynamics of the PPO gene family (StPPOs) gene expression in pigmented potatoes remains poorly characterized. Here, we present an integrated biochemical and molecular analysis of two purple-fleshed Peruvian landraces (Siriñacha and Angashungo), a partially pigmented landrace (Sapa), and non-pigmented cultivars, including the commercial cultivar Desirée. We quantified the total phenolic content, antioxidant capacity, and enzymatic browning index (EBI) using colorimetric and spectrophotometric methods. We also generated gene expression profiles of ten StPPO genes using semi-quantitative and digital PCR. Purple-fleshed cultivars exhibited significantly higher phenolic content and antioxidant capacity but also displayed accelerated browning kinetics compared to non- or partially pigmented genotypes. Expression analysis revealed cultivar-specific StPPO patterns, with StPPO2 and StPPO8 being markedly upregulated in pigmented materials, particularly StPPO8. These findings provide the first integrated biochemical and transcriptional evidence linking specific StPPO isoforms to enzymatic browning in colored potatoes, and highlight their potential for biotechnological applications. Full article

This study explores how demystifying Earth Observation (EO) through co-creation pathways and local language can enhance flood resilience and environmental governance in African informal cities. Using case studies from Maiduguri and Hadejia, Nigeria, the research employed a transdisciplinary mixed-methods design combining rapid evidence assessment, surveys, participatory workshops (n = 50 stakeholders) integrating simplified Sentinel-1/2 demonstrations, indigenous knowledge mapping, and pre-/post-engagement surveys on EO familiarity. Non-expert participants were trained to interpret satellite data using local language, linking distant teleconnections with local flood experiences. The findings revealed significant gains in EO literacy and improvements in interpretive confidence, gender-inclusive participation, and policy engagement. Localizing the curriculum enabled participants to translate technical EO concepts into locally meaningful narratives, fostering cognitive empowerment and practical application in flood preparedness and advocacy. The study demonstrates that data democratization is not only a matter of open access but also of open understanding. It advances a conceptual model linking Demystification, Literacy, Empowerment, Co-Production and Resilience, positioning EO as a social technology that bridges scientific and indigenous knowledge systems. The findings contribute to debates on decolonizing environmental science and propose a potential participatory framework for integrating EO into community-based adaptation, legal accountability, and policy reform across Africa’s rapidly urbanizing landscapes. Full article

Background: Severe Alpha-1-Antitrypsin deficiency (AATD), phenotype PiZZ, is a leading cause of liver disease in neonates, children, and adults. Nevertheless, the prevalence of liver disease and mortality within PiZZ adults remains unclear. Between 1972 and 1974, a cohort of 129 individuals with severe AATD (PiZZ) was identified through the Swedish national screening of 200,000 newborns. The cohort has been followed up regularly since birth. This prospective cohort follow-up study, with a cross-sectional comparison at 50 years of age, aims to characterize the natural history of liver disease and mortality in this cohort in their early fifties, compared with an age-matched control group (PiMM) randomly selected from the population registry. Methods: Study participants completed questionnaires regarding occupation, medical history, medication, and alcohol consumption. They underwent physical examination and measurement of liver stiffness using transient elastography (TE, FibroScan^®). Blood samples were obtained for evaluation of liver function, alcohol consumption, calculation of liver fibrosis scores, and detection of viral hepatitis and autoimmune liver disease. Results: Ninety-five PiZZ and 124 PiMM individuals participated in the study, of whom 47 PiZZ and 96 PiMM underwent TE measurement. PiZZ individuals had significantly higher median liver stiffness compared with PiMM individuals (5.9 kPa vs. 4.5 kPa, p < 0.01). No significant differences were found in Fib-4 score or the Non-Alcoholic Fatty Liver Disease Fibrosis Score (NFS) between the groups. Since identification of the cohort at birth, 13 (10%) of the 129 PiZZ individuals have died. Of these, liver disease was the main or underlying cause of death in 8 individuals (6%). Conclusions: In their early fifties, PiZZ individuals show a small but significant increase in liver stiffness measured by TE, indicating early liver fibrosis. In contrast, conventional fibrosis scores, such as Fib-4 and NFS, do not differ between PiZZ individuals and PiMM, suggesting that serum-based fibrosis scores may underestimate fibrosis in AATD. In this cohort, liver disease and its complications represented the main cause of death in PiZZ individuals by the age of 50, an observation that is uncommon in the general population at this age. Full article

Water is a vital resource that requires long-term legal protection to ensure both ecological values and societal benefits. The European Union’s Water Framework Directive (2000/60/EC) is central to this aim, establishing binding requirements for good ecological and chemical status in all water bodies and legally binding environmental quality standards. Sweden has implemented the Directive into national law; however, its application has been characterized by legal ambiguities, particularly regarding the possibility of considering disproportionate costs in environmental measures. This study examines the scope and application of the disproportionate cost criterion within the context of environmental law and hydropower regulation in Sweden. A comparative overview of the criterion’s application in other EU/EEA countries is also provided. Based on a legal approach, the analysis focuses on how these rules affect hydropower, where the goal of renewable energy production often needs to be weighed against the requirement for ecological recovery. The study concludes that applying the disproportionate costs criterion requires transparency and legal certainty to ensure a fair balance between the social benefits of hydropower and the need for long-term protection of the aquatic environments. To avoid differences in how the criterion is applied in different EU Member States, harmonized guidelines are needed. Full article

Protein-losing enteropathy (PLE) is an uncommon and often underrecognized manifestation of lymphoproliferative disorders and may be difficult to diagnose when conventional gastrointestinal investigations are unrevealing. We present an 82-year-old woman with recurrent hospital admissions initially spanning six months for diarrhea, weight loss, peripheral edema, and persistent hypoalbuminemia. Initial upper gastrointestinal endoscopy was normal, and colonoscopy was deferred due to intercurrent infection. Despite extensive laboratory and radiologic evaluation, including routine biochemical testing and imaging, the etiology of PLE remained unclear. Peripheral blood flow cytometry subsequently identified a small kappa-restricted monoclonal B-cell population compatible with marginal zone lymphoma, later confirmed on bone marrow biopsy, raising suspicion for gastrointestinal involvement. Video capsule enteroscopy demonstrated diffuse erosive and ulcerative disease throughout the small intestine, providing an anatomical explanation for the patient’s protein loss. Following lymphoma-directed therapy, repeat capsule enteroscopy showed complete normalization of the small bowel mucosa. This case highlights the diagnostic value of combining peripheral blood flow cytometry and capsule endoscopy in unexplained protein-losing enteropathy, a rare and diagnostically challenging presentation of indolent lymphoma, and illustrates the role of capsule imaging in both disease localization and treatment monitoring. As a single-case report, these findings are not generalizable, and further studies are required to evaluate the broader applicability of this diagnostic approach. Full article

Endometriosis affects approximately 10% of reproductive-age women and is associated with genomic instability; however, the contribution of specific DNA repair deficiencies remains poorly understood. This study investigated the expression and function of GEN1, a Holliday junction resolvase critical for homologous recombination, in patient-derived endometrial epithelial organoids (EEOs). Endometrial tissue was obtained by pipelle biopsy from women with laparoscopically confirmed endometriosis (n = 3, stage III–IV) and controls without endometriosis (n = 3). GEN1 mRNA and protein expression were reduced in primary endometrial cells from endometriosis patients compared with controls (mRNA: 0.52 ± 0.14 vs. 1.00 ± 0.19, p = 0.05; immunofluorescence intensity: 0.54 ± 0.18 vs. 1.00 ± 0.22, p = 0.05). Patient-derived EEOs from the endometriosis group showed trends toward lower formation efficiency (18.4 ± 5.6% vs. 25.2 ± 6.8%, p = 0.10) and reduced mean diameter (124.6 ± 34.2 vs. 155.8 ± 32.6 µm, p = 0.10). RNA interference (RNAi)-mediated GEN1 knockdown reduced proliferation in both groups, with a more pronounced effect in endometriosis-derived EEOs (49.7% vs. 39.5% reduction, p = 0.05). Endometriosis-derived EEOs exhibited elevated baseline γH2AX (phosphorylated histone H2AX) immunofluorescence compared with controls (2.32 ± 0.44 vs. 1.00 ± 0.28, p = 0.05), indicating increased DNA double-strand break accumulation. Furthermore, GEN1 knockdown directly increased γH2AX intensity in both groups, with endometriosis-derived EEOs showing a greater absolute increase (Δ1.26 vs. Δ0.72). To our knowledge, this study provides the first organoid-based evidence that GEN1 is downregulated in endometriosis and functionally linked to impaired proliferation and elevated DNA damage, suggesting a potential contribution of homologous recombination dysregulation to endometriosis pathogenesis. Full article

Background: The pathophysiological mechanisms of Abdominal Aortic Aneurysm (AAA) are not elucidated. Alterations in mitochondrial function, such as a reduction in oxidative phosphorylation (OXPHOS), have been observed at genome level and functionally in vascular smooth muscle cells. Metformin reduces AAA development and growth in diabetic patients, but the precise mechanisms are not known. In this paper we aim to demonstrate the feasibility of measuring mitochondrial functional capacity ex vivo in intact murine aneurysmal tissue and confirm a decrease in OXPHOS, and to determine if the protective effect of metformin on AAA is mediated by mitochondrial function. Methods: AAA was induced in ApoE KO mice by administration of angII (1000 ng/kg/min) through osmotic minipumps. Metformin was administered in drinking water at a dose of 100 mg/kg/day. The abdominal aorta was isolated in situ and mitochondrial functional capacity was analyzed ex vivo in whole permeabilized tissue by high-resolution respirometry. Results: Mitochondrial respiration was successfully measured ex vivo in whole aneurysmal tissue. Mitochondrial function was impaired in angII-treated mice, with decreased fold change in Complex I and Complex I+II oxygen consumption, relative to basal levels. Complex II oxygen consumption was also decreased in angII-treated mice. Rescue treatment of mice with metformin did not affect or restore mitochondrial function. Conclusions: Mitochondrial function can be evaluated in murine whole aneurysmal tissue, providing a method for a physiological approach to the study of mitochondrial function in AAA. Mitochondrial function is impaired in AAA. However, rescue treatment with metformin is not sufficient to recover mitochondrial function and seems not to be the mechanism behind prevention of aneurysm. Full article

Parabens, a prevalent class of endocrine-disrupting chemicals (EDCs), are ubiquitous in consumer products; however, their role in linking pediatric allergic phenotypes remains poorly understood. This case-control study analyzed paraben levels in urine and indoor dust as proxies for internal and external exposures and investigated their associations with allergic rhinitis only (AR Only), asthma only (AS Only), and comorbidities (AR&AS) among children in Shanghai. The concentrations for each of four paraben compounds were quantitatively measured, and multi-pollutant frameworks—including Bayesian Kernel Machine Regression (BKMR) and Weighted Quantile Sum (WQS) regression—were employed to characterize the mixture exposure and risk. Propylparaben (PrP) was detectable in 100% of urine samples and over 90% of dust samples, and the concentrations ranked the highest out of the four compounds in both samples. Benzylparaben (BzP) was detected in >70% of urine samples and over 50% of dust samples at relatively lower levels. Urinary PrP exhibited significantly positive associations with all phenotypes (OR in 2.18–2.92) and BzP with the AR&AS Comorbidity (OR = 3.55, 95% CI: 1.32–9.55). Dust-borne PrP was associated with AR Only (OR = 2.26, 95% CI: 1.16–4.43), indicating a potential “Portal of Entry” effect via direct nasal deposition. According to BKMR and WQS analyses, urinary PrP and BzP emerged as two primary risk drivers. Using interaction analysis, an additive synergistic effect was observed between urinary PrP and BzP with parental history of allergy, suggesting heightened vulnerability to paraben exposure in genetically predisposed subgroups. In conclusion, children with respiratory allergies were associated with higher exposure to PrP and BzP and exhibited higher susceptibility in those with a parental history of allergy. Full article

Mastitis in dairy cows, caused by pathogens such as Staphylococcus aureus, Escherichia coli, and Candida albicans, leads to substantial economic losses and contributes to antimicrobial resistance, emphasizing the need for natural alternatives. This study assessed the phytochemical composition, antioxidant activity, and in vitro antimicrobial effects of ethanol extracts from ten medicinal plants, including Populus balsamifera buds, Syzygium aromaticum, and Humulus lupulus, as well as two multi-component plant mixtures and commercial products against reference strains and field isolates of mastitis pathogens. Extracts exhibited total phenolic contents ranging from 2.5 to 43.0 mg GE/g, with Populus balsamifera and Syzygium aromaticum demonstrating the strongest DPPH radical scavenging activity (IC₅₀ 1.89–2.9 mg/g extract). Disc diffusion assays demonstrated broad-spectrum inhibition, particularly for Populus balsamifera (15.1–19.1 mm inhibition zones) and Humulus lupulus (9.0–18.4 mm) against key pathogens; phenolic and flavonoid contents positively correlated with antimicrobial activity (r = 0.63–0.99, p < 0.001). Multi-component mixtures provided consistent broad-spectrum effects (12.6–17.2 mm). These phenolic-rich plant extracts represent promising alternatives to reduce antibiotic use in dairy mastitis management. Full article

One of the common issues in the R&D of new drugs is the failure of clinical trials caused by the species-specific inadequacy of animal models to assess drugs’ efficiency and safety. Therefore, systems like organ-on-a-chip and, particularly, liver-on-a-chip (LOC) can be an efficient tool for recapitulating in vivo-like human physiology at the microscale. This review focuses on discussing LOC design, emphasizing its architecture and validation to reveal the trends in searching for a balance between biomimetics and functionality. We found that the huge variety of already published models can be divided into five groups based on their configuration complexity: flat one-channel, flat two-channel, vertically stacked multilayered, hexagonal-patterned, and multi-well chips. While researchers attempt to recapitulate the liver’s histology and its functions in detail by increasing the complexity of devices’ architectonics, industrial companies prefer to promote more simple and flexible solutions. Thus, the LOC designs of the future require neglecting some liver characteristics to make them standardizable and sustainable, which could facilitate their introduction into the market and clinics. Full article

Technological advances such as Vehicle-to-Grid (V2G) have the potential to support renewable energy integration and grid stability, but large-scale deployment depends on users’ willingness to participate, particularly in public charging environments. While prior research has examined V2G from technical feasibility and system-level perspectives, everyday public settings remain unexplored. This study investigates electric vehicle (EV) users’ willingness to engage in V2G services in public spaces, with a focus on incentives, expectations, and how participation aligns with existing routines and parking conditions. A mixed-method approach was applied, combining a survey of 544 car users with two waves of user-centered interviews. The survey data were analyzed using factor analysis and linear regression models, while the interview data were thematically analyzed. The results show that users’ evaluations of V2G are shaped by sustainability expectations, perceived efficiency, and uncertainties, and preferences for public V2G participation are strongly influenced by convenience, clarity of the offer, and perceived control. Home charging practices emerged as a key reference point shaping expectations of public V2G services. Across both methods, simple and transparent incentives, such as reduced charging or parking costs, were consistently preferred over more complex reward models, including point-based systems or dynamic energy trading. Concerns related to control over trips, battery degradation, trust in service providers, and added complexity remain important barriers to participation. The findings highlight the need for user-centered and socio-technical design of public V2G services that align with users’ everyday routines, parking conditions, and expectations to support broader adoption beyond the home context. Full article