Search Results (79)

To determine Fusarium species and their pathogenicity in maize-production areas of the Tepebaşı, Odunpazarı, Alpu, and Seyitgazi districts of Eskişehir province, Türkiye, 180 samples were collected from 45 fields during survey studies conducted in 2023–2024. A total of 110 Fusarium isolates were obtained from the collected plant samples. The isolates were identified as F. verticillioides, F. culmorum, F. proliferatum, F. graminearum, F. sambucinum, F. acuminatum, F. chlamydosporum, and F. equiseti. The most common species was F. verticillioides, while the most virulent species was F. graminearum, with a disease severity of 96.67%. The effects of different doses of soil-applied herbicides containing the active ingredients Isoxaflutole + Thiencarbazone-methyl + Cyprosulfamide, Dimethenamid-P + Saflufenacil, and S-Metolachlor + Terbuthylazine on F. graminearum were evaluated under both in vitro and in vivo conditions. Under in vitro conditions, the highest inhibition rate (57.23%) was observed in the double-dose application of the herbicide containing S-Metolachlor + Terbuthylazine. This was followed by the upper and recommended doses of the same herbicide with inhibition rates of 47.16% and 39.46%, respectively. For the other herbicides, inhibition rates increased with increasing herbicide dose. In field trials, the highest suppression of the pathogen was also observed with the herbicide containing S-Metolachlor + Terbuthylazine. While the recommended dose showed a 38.6% effect against the pathogen, the upper dose resulted in a 45.31% effect. This study suggests that herbicide applications may be associated with improved plant growth, likely due to reduced pathogen pressure and decreased weed competition. The findings highlight the complex interactions between soil-applied herbicides, soil-borne pathogens, and host plants, and provide insights into the development of integrated disease management strategies in maize-production systems. Full article

In this study, core–shell molecularly imprinted polymers (CS-MIP) were utilized for the detection of the herbicide S-metolachlor in surface water samples, collected from a river and pond that are in the proximity of cornfields. The study revealed that no traces of herbicide were detected in the samples that were analyzed. The collected water samples were treated with membrane filtration—microfiltration and ultrafiltration. The adsorption isotherms were fitted using the Langmuir, Freundlich, Dubinin–Radushkevich, and Scatchard models. This indicated that the Scatchard model is the most appropriate for CS-MIP. The data obtained from the kinetic study were analyzed using the pseudo-first-order and pseudo-second-order models, as well as Fick’s second law. For CS-MIP, the most suitable model was determined to be the particle diffusion model, while for core–shell non-imprinted polymers (CS-NIP), the film diffusion model was identified as the limiting step. A method for the desorption of S-metolachlor from the pH-sensitive sorbent bed has been developed, thereby enabling the material to be reused. The optimum eluent from the multicomponent solution was determined to be a 30% aqueous ethanol solution with a pH of approximately 9. This solution effectively removed the majority of contaminants, with the exception of S-metolachlor, which was retained within polymer pores. Full article

Sustainable agriculture is increasingly reliant on reducing anthropogenic inputs and recycling organic waste while protecting ecosystems. In this context, this study investigated the agro-environmental properties of biosulfate, focusing on its interaction with herbicides and its effects on soil fungi and horticultural plants. Two biosulfate samples obtained from urban sewage sludge from the Barletta (BIO-BA) and Foggia (BIO-FO) treatment plants were characterized by Fourier transform infrared–attenuated total reflectance (FTIR-ATR) spectroscopy and scanning electron microscopy (SEM). The adsorption/desorption of the herbicides metribuzin (MET), S-metolachlor (S-ME) and cycloxydim (CYC) on biosulfates was evaluated by studying adsorption kinetics and isotherms. All herbicides reached adsorption equilibrium within a few hours, according to pseudo-second-order kinetics, indicating a predominant chemical interaction between biosulfate and the molecules. Considering the organic C content of BIO-BA (~21%) and BIO-FO (~17%), which was less than half that commonly measured for other organic fertilizers, such as compost and digestate, their adsorption capacity was high, with Freundlich adsorption constants ranging from 772 µg g⁻¹ (S-ME on BIO-BA) to 1464 µg g⁻¹ (CYC on BIO-FO). A low hysteresis coefficient indicated a rather slow and incomplete release of the molecules from the biosulfate. Exposure of the fungi Pleurotus ostreatus and Pleurotus eryngii to 1, 2, 3, and 4% BIO-BA and BIO-FO stimulated mycelium growth, indicating that responses depended on fungal species and biosulfate dose. Finally, germination and early growth of lettuce and basil were generally unaffected by either biosulfate, as parameters such as germination percentage, root and shoot length, and fresh and dry biomass were not statistically different from the control. Some growth stimulation was observed in basil. Overall, biosulfate appears to be a promising soil fertilizer, as it can contribute to soil organic matter, retain xenobiotics, and exert biostimulatory effects under controlled conditions. Full article

Recycled bio-wastes such as compost and vermicompost, and bioenergy byproducts such as digestate and biochar are widely acknowledged for their role as soil conditioners capable of preserving soil fertility, maintaining soil health, and acting as a bio-adsorbent of organic soil pollutants (BIOSORs). Moreover, they are attracting increasing attention for use as effective carriers of microbial consortia into arable soils. This study aims to combine selection of bacteria tolerating contaminants of emerging concern (CECs) and their use to fortify BIOSORs. Seventeen bacterial strains isolated from commercial bio-stimulant formulations were studied together with three strains previously isolated and identified as Bacillus subtilis, Bacillus licheniformis, and Serratia plymuthica. All the strains were tested in vitro for their ability to grow under increasing concentrations (0, 0.2, 0.5 and 1 mg L⁻¹) of CECs: bisphenol A, 4-nonylphenol, penconazole, and S-metolachlor. Results highlighted a variability in the tolerance of the bacteria to the tested CECs. The B. subtilis, B. licheniformis, and S. plymuthica were the most promising strains, individually or as consortium, to tolerate individual CECs and their mix. Moreover, they exhibited metabolic activity when inoculated in the BIOSORs. Nevertheless, additional investigations such as quantitative assessment of CECs are needed to validate the methodology. This work contributes to investigate the feasibility of stable and functionally active microbially enriched bio-sorbents (Me-BIOSORs) and provides preliminary evidence supporting the potential to be used in soil–plant systems at the field scale. Full article

Weeds are among the primary constraints reducing soybean productivity, and their effective control is especially important in edamame, a vegetable soybean valued for its nutritional potential. As chemical control remains the dominant strategy, rational herbicide use is essential. This study aimed to evaluate the response of two edamame varieties (Chiba Green and Midori Giant) and the effectiveness of applied herbicides in weed control during the 2022–2024 growing seasons. Treatments included the following: pre-emergence herbicides (S-metolachlor + metribuzin) (H1); pre- (S-metolachlor + metribuzin) and post-emergence herbicides (imazamox + cycloxydim) (H2); and an untreated control (H0). The growing season influenced pod yield and biomass, with the highest yield recorded in 2022 (11.7 t ha⁻¹), while variety affected only pod yield: on average, Midori Giant outperformed Chiba Green (10.6 vs. 6.1 t ha⁻¹). Herbicide treatment affected weed dry biomass (3.3 g m⁻² in H2 compared to 341.8 g m⁻² in H0) and pod yield (4.3 t ha⁻¹ in H0 for Chiba Green compared to 11.9 t ha⁻¹ in H2 for Midori Giant). The results indicate that pre-emergence herbicides could satisfactorily reduce weed infestation under suitable meteorological conditions. The combined application of pre- and post-emergence herbicides increases production security (particularly in seasons with higher weed infestation), likely by extending the weed control period through pre- and post-emergence herbicide combinations, targeting different weed species during the soybean vegetative period. In addition, weed diversity was associated with a yield increase in Midori Giant. This research provides practical information and options for weed management in edamame production in the Western Balkan region. Full article

Metabolic disorders such as obesity have increased globally in recent decades and are a major public health concern. Previous research suggests that herbicide exposures may contribute to metabolic dysfunction, but few studies have examined mixture effects of multiple herbicides on obesity at a population level. Using county-level data from 2013 to 2018, we examined the associations between obesity rates and the application of 13 commonly applied herbicides in the U.S. We first conducted adjusted single-pollutant mixed-effects models and then used quantile-based g-computation mixture modeling to assess combined herbicide mixture effects on county-level obesity rates. Models were adjusted for demographic and socioeconomic covariates and accounted for geographic clustering. Significant positive associations were identified between county-level obesity rates and applications of glyphosate, 2,4-D, atrazine, acetochlor, metolachlor, and several other herbicides in adjusted single-pollutant models. Glyphosate showed one of the strongest individual associations (β = 0.29 per standard deviation increase, 95% CI: 0.21–0.36). Increases in herbicide mixture were significantly associated with higher obesity rates (Psi = 0.71 per quantile exposure mixture, 95% CI: 0.65–0.76) from mixture modeling. Inclusion of significant interaction terms did not appreciably increase the mixture effect. Glyphosate, 2,4-D, metolachlor, dimethenamid-P, and glufosinate contributed most strongly to the weighted mixture effect. Mixture effects varied by rurality, with stronger associations observed in rural counties, particularly in micropolitan regions. Our findings highlight the importance of considering cumulative herbicide mixture exposures rather than individual chemicals in isolation. The observed rural–urban disparities emphasize the need for targeted public health interventions and policy actions in rural communities, which may be particularly vulnerable to the adverse metabolic impacts of herbicide mixtures. Full article

Cyperus esculentus is a very destructive perennial weed, rapidly propagating and spreading through large amounts of daughter tubers. Successful control relies on depleting the soil tuber bank. This study investigated the effect of different control measures, applied across several cropping systems, on tuber bank dynamics over time. Therefore, 52 infested fields were monitored over 3 consecutive years, with annual quantification of the C. esculentus tuber bank. In maize monocropping systems, substantial 3-year tuber bank reductions (>90%) are achievable with preplant incorporation of dimethenamid-P or S-metolachlor, followed by a post-emergence application of mesotrione and pyridate at the 4–5 leaf stage, combined with delayed sowing (after 20 May) or mechanical measures (e.g., hoeing, harrowing). On non-maize fields, effective strategies (median tuber bank reductions of 57–70%) include intensive black fallow with at least four control timings or winter cereal cropping followed by intensive control (at least three measures) during the stubble phase. Established, fertilized grasslands also offer moderate reductions (17–67%) via intensive grazing or mowing. These results demonstrate that significant C. esculentus reductions are possible across different crops, but control remains challenging, requiring intensive, repeated strategies over multiple years. Less intensive approaches may undermine previous efforts. Full article

Aquatic ecosystems are threatened by various anthropogenic activities, including those exacerbated by pesticides leaching from agricultural lands. Although legislation and regulations regarding pesticides aim to eliminate the risk of eutrophication and pollution, only a few studies have examined the impact of these substances on non-target organisms, such as microalgae, which are highly involved in biogeochemical cycles and critical for ecosystem integrity. We studied the effect of the agricultural insecticide Teppeki based on flonicamid, the fungicide Ortiva with azoxystrobin, and the herbicide Basar with (S)-metolachlor on the green microalga Chlorella vulgaris and the cyanobacterium Synechococcus leopoliensis. Ortiva and Basar were more toxic at lower doses than Teppeki, with (S)-metolachlor demonstrating the most instantaneous and potent inhibition. Half maximum effective concentration (EC₅₀) values confirmed the strong inhibitory effect of the herbicide on both strains on days 3 and 8, and highlight the differing temporal responses, especially for Ortiva. This observed pattern of toxicity is consistent with pulse-amplitude-modulated fluorescence measurements of photosystem II, which indicate that both species are more sensitive to (S)-metolachlor and azoxystrobin than to flonicamid. We claim that the side effects of pesticides on non-target organisms must be given more attention. It is well established that herbicides can impair photosynthetic organisms such as microalgae, but pesticides targeting other pests can also cause adverse effects on these communities. Such unwanted side effects are directly related not only to the reduction of biodiversity, but also to human health. Full article

This study examined the effects of 2, 4 and 8% digestate-derived compost (DCP) on the retention/release of the fungicide penconazole (PEN), the herbicide S-metolachlor (S-MET) and the endocrine disruptor bisphenol A (BPA) in two agricultural soils sampled in Valenzano (SOV) and Trani (SOT), in Sothern Italy. DCP alone showed a conspicuous adsorption of the three xenobiotics, followed by their slow and scarce release. Sorption isotherm data of the compounds on unamended and DCP-amended soils were well described by the Freundlich model. Compared to unamended soil, the addition of the highest dose (8%) DCP to SOV increased the distribution coefficient, Kd, values of PEN, S-MET and BPA by 281%, 192% and 176%, respectively, while for SOT, the increases were 972%, 786% and 563%, respectively. Desorption of PEN and S-MET from all treatments was slow and partial (hysteresis), and only slightly reduced or unaffected by the addition of DCP, whereas BPA was almost entirely undesorbed in all treatments. Highly significant correlations between the adsorption coefficients of the three compounds in all soil treatments and the corresponding organic C contents confirm the prominent role of native and anthropogenic OM in the adsorption of contaminants and, consequently, in the control of their transfer into natural waters and/or entry in crop plants. Full article

This study analyzed the use of a toolbox to evaluate the impact of agricultural activity on the water quality/status classification of a hydro-agricultural reservoir (Lage reservoir, Southern Portugal). The framework integrated the quantification of a group of 51 pesticides and ecotoxicological endpoints with organisms from different trophic categories (the bacterium Aliivibrio fischeri, the microalga Pseudokirchneriella subcapitata, and the crustaceans Daphnia magna and Thamnocephalus platyurus) at two sampling points in the reservoir (Lage (L) and Lage S (LS)) between 2018 and 2020. Over the three-year study, we quantified 36 of the 51 pesticides analyzed in the Lage reservoir. Total concentrations increased successively from 0.95 µg L⁻¹ to 1.99 and 2.66 µg L⁻¹. Among these, the pesticides most frequently detected were terbuthylazine (100% of detection) and metolachlor (83% of detection), with maximum concentrations of 115.6 and 85.5 µg L⁻¹, respectively. Samples from the LS site showed higher toxicity, where A. fischeri presented 30 min EC₅₀ values of 39–51%. Microalgae growth was consistently inhibited, correlating with agricultural activity, mainly the application of herbicides and insecticides, while D. magna feeding rates revealed no inhibitory effects in the Lage samples. The results highlight that although the detected pesticide levels were below regulatory limits, they still induced toxic effects in the tested organisms. The potential ecological status of the reservoir was classified as moderate, and the integration of the proposal toolbox allowed refinement of the classification of water status. The results demonstrated that this integrated approach, combining multiple assessment methods, establishes a more robust water quality evaluation methodology, allowing it to be used as a tool complementary to the WFD methodology. This proposal not only identified existing pollution impacts but also enabled (1) early detection of the toxic effects of emerging contaminants to prevent ecological damage; (2) proactive management through specific actions to restore water status; and (3) improved sustainable water use. Full article

Pesticides play a significant role in agriculture, but their leaching into soil and water poses serious environmental risks. This study examines pesticide contamination in surface and groundwater in northern Tunisia, specifically in Kef governorate, involving a survey of 140 farmers to gather data on agricultural practices and pesticide use. Twenty-four pesticides were monitored and utilized within the Pesticide Environmental Risk Indicator (PERI) model to evaluate environmental risk scores for each substance. Soil and water samples were analyzed using a multi-residue method and liquid chromatography–tandem mass spectrometry. Results showed that 50% of the pesticides assessed had an Environmental Risk Score of 5 or higher. Contamination was identified in water and soil, with 18 and 15 pesticide residues, respectively. Notable concentrations included 7.8 µg/L of linuron and flupyradifurone in water and 1718.4 µg/kg of linuron in soil. Commonly detected substances included the insecticide acetamiprid and fungicides like cyflufenamid and penconazole in water, while soil contamination was linked to fungicides metalaxyl and metalaxyl-m, as well as herbicides linuron and s-metolachlor. Factors such as proximity to treated water points and poor packaging management were discussed as risks. The findings emphasize the need for better monitoring and sustainable agricultural practices to mitigate contamination. Full article

Soybean production in Thailand faces significant challenges from malignant weed competition, potentially reducing yields by up to 37% and incurring annual economic losses of approximately USD 3.8 billion. Pre-emergence herbicides are critical for integrated weed management, but their efficacy varies depending on local conditions and soybean varieties. This study evaluates the performance of three pre-emergence herbicides, pendimethalin (1875 g a.i. ha⁻¹), s-metolachlor (900 g a.i. ha⁻¹), and flumioxazin (125 g a.i. ha⁻¹), on weed control efficiency (WCE), soybean growth, phytotoxicity, and yield in Northeast Thailand using a randomised complete block design with two varieties (CM60 and Morkhor60) across rainy (2023) and dry (2024/2025) seasons. Herbicide performance varied seasonally: s-metolachlor showed optimal rainy season results (61.54% weed control efficiency at 63 days after herbicide application (DAA), with a yield of 1036 kg ha⁻¹), while flumioxazin excelled in dry conditions (64.32% WCE, <4% phytotoxicity, and 1243 kg ha⁻¹ yield). Pendimethalin performed poorly under wet conditions but improved in drier weather. Among five dominant weed species, Cyperus rotundus proved the most resilient. CM60 demonstrated superior herbicide tolerance and yield stability, particularly under rainy conditions. These results emphasise that season-specific herbicide selection and variety matching are crucial for herbicide resistance management and effective weed control in Thailand’s rainfed soybean systems. Full article

Tomato is a major crop, and efforts are ongoing to enhance its resilience to biotic and abiotic stresses. Weed management remains a key challenge, prompting the search for sustainable alternatives to reduce the impact of excessive herbicide use. Biochar is a promising alternative, as it enriches the soil, improves its water retention capacity, promotes its regeneration and increased fertility, delays nutrient leaching, and improves fertilizer use efficiency. This study aimed to investigate the efficiency of biochar use in mitigating stress caused by different herbicides. Two different biochar materials, Douglas fir and rice husk, were used. Tomato seeds were sown in pots and arranged in a randomized design. At the 4V stage (28 days after sowing), the herbicides S-metolachlor, metribuzin, and halosulfuron were applied. Plant length, injury, antioxidant enzyme activity, ascorbate peroxidase (APX), catalase (CAT), guaiacol peroxidase (GPOD), glutathione reductase (GR), and hydrogen peroxide content (H₂O₂) were assessed 7 and 14 days after herbicide application. Plants treated with biochar and submitted to herbicide treatments showed significantly higher growth parameters and fewer injuries when compared to plants treated with herbicides without biochar. The antioxidant response of the plants followed the same trend; smaller plants with more injuries showed greater H₂O₂ accumulation and significantly higher antioxidant enzyme activity. These findings highlight the protective effect of biochar, particularly Douglas fir biochar, as it effectively mitigated herbicide-induced oxidative stress and helped maintain plant growth and structural integrity under treatment conditions. Full article

Seed production of common vetch (Vicia sativa L.) and sweet white lupine (Lupinus albus L.) is risky due to weed infestation as few herbicides are permitted for use in crops. Our aim was to test herbicides in these crops in order to expand the list of available herbicides. Various pre- and post-emergence herbicides were tested for their phytotoxicity and weed-control activity in field cultures of the common vetch (cv. Emma) and sweet white lupine (cv. Nelly). After the application of herbicides, phytotoxicity was monitored visually. Data collection involved the Normalized Difference Vegetation Index (NDVI), the plant height, the number of weeds, yield, and its contamination. Additionally, 1000-seed-weight measurements were taken for lupine. Summarizing the phytotoxicity and efficacy results in common vetch, the agents S-metolachlor, flumioxazin, and clomazone can be recommended for further pre-emergence testing, while metazachlor + quinmerac, chlorotoluron, and flumioxazin can be recommended for further post-emergence testing. In sweet white lupine, pre-emergence applications of flumioxazin, pendimethalin, dimethenamid-P, pethoxamid, clomazone, metobromuron, and diflufenican were found to be effective without any significant phytotoxicity. Further post-emergence testing of flumioxazin, chlorotoluron, carfentrazone-ethyl, and diflufenican can also be recommended, as well as the application of halauxifen-methyl and sulfosulfuron at low doses (0.4 L ha⁻¹; 13.0 g ha⁻¹). Additional evaluations of these treatments are recommended, including in different soil and weather conditions. Full article

Annual grass weeds can provide significant competition to an establishing sown tropical perennial grass pasture. At least two years of grass weed control prior to sowing is required to reduce the weed seed bank. Pre-emergent herbicides used in summer cereals, such as atrazine or s-metolachlor with metcamifen seed safener, may reduce this preparation time. Two controlled-environment experiments were conducted to assess the potential for these pre-emergent herbicides to be used with several tropical perennial grasses. Experiment 1 tested the effect of metcamifen (400 g L⁻¹ a.i. at 0–2× label rate) on the emergence and vigor of Chloris gayana, Dichanthium aristatum, Digitaria eriantha and Panicum coloratum, with Sorghum bicolor as the control. Experiment 2 tested the effect of s-metolachlor (960 g ha⁻¹ a.i.) with metcamifen-treated or untreated seed, and atrazine (1800 g ha⁻¹ a.i.) on the emergence and early growth of the grasses. Metcamifen did not inhibit emergence or vigor of the grasses. Without metcamifen seed treatment, s-metolachlor reduced the growth of the tropical perennial grasses by 47–100%, while it had no such effect on S. bicolor. In contrast, there was no effect of atrazine on shoot yields of the grasses, nor of s-metolachlor when D. aristatum, D. eriantha and P. coloratum seed had been treated with metcamifen. The collective results indicate that the herbicide safener metcamifen does not reduce the viability of tropical perennial grass seed and provides some protection against s-metolachlor, albeit not complete protection at the rates used in our study. Atrazine did not affect emergence or early growth of the grasses. Full article