Search Results (58)

Global food security and dietary diversity depend on identifying novel and sustainable food sources. Wild edible plants (WEPs) traditionally used in Mediterranean regions offer considerable potential due to their rich history of use. Here, ethnobotanical knowledge was systematically compiled for the fruits and cones of five taxa (Arbutus unedo, Prunus spinosa, Quercus spp., Pinus spp. and Rosa spp.), documenting alimentary uses, preparation and conservation methods across diverse food categories. Analysis of over 2800 traditional use reports identified 54 distinct alimentary uses from 16 categories, with raw consumption and sweet preserves being the most prevalent. Rosa spp. exhibited the highest diversity of uses (36), whereas the family Pinaceae showed the lowest (19). Statistically significant associations between individual fruits and specific food preparations were also observed, offering guidance for innovative product development. Information on processing methods that preserve nutritional components, along with documentation of potential harmful effects and the methods to mitigate them, was collected, providing essential guidance for developing safe and functional alimentary products. Together, traditional knowledge, regulatory adherence, and sustainable practices create new opportunities to develop innovative, safe, culturally grounded, and sustainable food products that enrich diets and preserve cultural and ecological heritage. Full article

Prunus spinosa L. is a shrub with nutritional potential, yet limited information is available on the composition of its stone fruit and leaves. This study aimed to investigate and compare the macro and micronutrients, fatty acid profile, and lipophilic and hydrophilic antioxidant compounds in fruits and leaves, as well as their potential functional properties. The results revealed that leaves contain higher crude protein (10.94%) than fruits (6.46%) but lower crude fiber (13.86% compared to 22.16%). The iron (370.37 mg/kg) and manganese (43.57 mg/kg) were significantly higher (p < 0.05) in leaves than in fruits (44.87 versus 7.02 mg/kg). The fruits’ lipid profile was rich in monounsaturated fatty acids (56.8%), whereas the leaves showed higher saturated fatty acids (38.3%) and polyunsaturated fatty acids (43.7%). The leaves also presented significantly higher n-3 content (25.2%) compared with fruits (1.80%). The antioxidant compounds were higher in the leaves, with total vitamin E exceeding 1268 mg/kg, primarily α-tocopherol (1214.98 mg/kg) isoform, lutein (409.38 mg/kg), and astaxanthin (3.74 mg/kg), compared with only 74.75 mg/kg total vitamin E in the fruits. The total hydroxycinnamic polyphenols in leaves were 92.63% higher in leaves than in fruits, with anthocyanins at 63.23% and flavonols at 95.82%. Although the leaves demonstrated superior antioxidant potential and mineral content compared to the fruits, making it a promising candidate for nutraceutical and functional food applications, the fruits maintained a healthier lipid profile suitable for dietary fat intake. This comparative analysis highlights the distinct nutritional and bioactive composition of Prunus spinosa co-products. Full article

(1) Background: Prunus spinosa L. is known for its polyphenolic profile, including condensed tannins, compounds associated with various biological activities, including antiproliferative effects. Its woody biomass, such as branches, remains largely underexplored, as a few studies have investigated its potential bioactive content. This study aimed to characterize and evaluate the biological potential of crude extract (PS) obtained from P. spinosa branches. (2) Methods: The extract (PS) was prepared using microwave-assisted extraction (MAE) under optimized green conditions (70% ethanol, 1/10 solid–liquid ratio, 5 min, 600 W). Its chemical profile was analyzed by High-Performance Thin-Layer Chromatography (HPTLC). Antioxidant capacity was assessed through HPTLC-DPPH, DPPH and ABTS assays. In vitro cytotoxicity and genotoxicity were evaluated on HeLa (tumoral) and Vero (normal) cell lines using MTT and Comet assays. (3) Results: HPTLC analysis revealed the presence of condensed tannins. The extract demonstrated potent radical scavenging activity (IC₅₀ 1.02 ± 0.25 mg/mL), dose-dependent cytotoxicity, and higher sensitivity of HeLa cells. Genotoxic effects were significantly more pronounced in tumor cells than in normal ones. (4) Conclusions: These findings highlight the condensed tannins’ phytochemical profile, antioxidant and selective antitumor properties of PS, supporting its valorization as a sustainable source of multifunctional bioactive compounds. Full article

Blackthorn (Prunus spinosa L.) is a wild, understudied plant rich in bioactive compounds such as polyphenols with designated antioxidant potential. The main objective of this research was to optimize ultrasound-assisted extraction of blackthorn pomace using natural deep eutectic solvents (NADES). To obtain the highest yield of polyphenols and improved in vitro antioxidant activity, response surface methodology (RSM) and central composite experimental design were used. The screening step of the study included ten different NADESs using a one-factor-at-a-time approach. Two NADES mixtures (N12, containing proline and lactic acid in a molar ratio of 1:2, and N14, containing choline chloride and glycerol in a molar ratio of 1:1) were chosen for the second step of the study, which aimed to select the most influential process parameters. A fractional factorial 2⁵⁻¹ design was used, varying five different parameters at two levels: extraction time (30 and 60 min), extraction temperature (40 and 50 °C), and liquid-to-solid ratio (10 and 20 mL/g), water content in NADES (15 and 20%), and NADES type (N12 and N14). After the second step, N12 containing 20% water was chosen as the most potent solvent for the optimization study. For the final step, the other three parameters were varied on three levels, and thus optimal conditions were obtained (extraction time 90 min, extraction temperature 65 °C, and liquid-to-solid ratio 22.65 mL/g). Blackthorn juice was also tested in the first step, as well as under optimal conditions established for pomace, in order to evaluate whether these conditions are suitable for juice and to determine the percentage of improvement in extraction efficiency. Full article

Background: Antibiotic resistance is spreading, and the effectiveness of the most widely used antibiotics is decreasing. These issues are global health and food safety concerns that require immediate attention. One potential solution is the use of various gemmotherapy extracts (GTEs). However, there is a paucity of studies investigating the presumptive antimicrobial activity of GTEs. Methods: In this comparative study, we are assessing the antimicrobial properties of eight selected GTEs, as well as their polyphenol content and antioxidant activity, against a panel of microorganisms (Gram-positive and Gram-negative bacteria, yeasts, and molds). We are using the agar diffusion method (ADM) and the broth microdilution method (BMD) to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). Results: Among the analyzed extracts, dog rose, lingonberry, sea buckthorn, blackthorn, and common grape GTEs showed the highest total phenolic content, antioxidant activity, and the most relevant antimicrobial activity including certain differences with respect to the microbiostatic and/or microbicidal properties. These results demonstrate the relative strength of the antimicrobial effects of specific GTEs against certain microbial species, which could facilitate the use of these GTEs in personalized and/or specific antimicrobial therapies. Full article

The grapevine microbiome is shaped by a complex interplay of biotic and abiotic factors, affecting microbial community structure and plant health. This study investigates the diversity, composition, and dynamics of fungal communities associated with grapevine (Vitis vinifera) and neighboring cultivated plants, as well as plants from semi-natural vegetation, including pear (Pyrus communis), apricot (Prunus armeniaca), dogrose (Rosa canina), and blackthorn (Prunus spinosa), in a landscape-level habitat matrix. Using metabarcoding techniques, fungal communities from leaves and woody tissues of grapevine and neighboring plants were analyzed over a growing season. Fungal richness and abundance differed significantly among host plants, with woody tissues exhibiting higher diversity. Host plant identity was the primary factor shaping wood-associated fungal communities (15.7% of explained variance), whereas sampling time dominated in leaves (16.3%), with sampling site having a weaker effect in both cases. Pathogenic fungi associated with grapevine trunk diseases, such as Diaporthe, Eutypa, and Phaeomoniella, were identified across grapevine and neighboring plants, suggesting that multiple hosts may act as reservoirs for fungal inoculum. These findings highlight the complex interactions between fungal communities, host plants, and environmental factors, underscoring the need for landscape-level approaches to plant protection that account for both cultivated and surrounding ecosystems. Full article

Blackthorn (Prunus spinosa L.) fruits are traditionally used to produce tinctures and are known for their high content of bioactive compounds with antioxidant, anti-inflammatory, and antimicrobial effects. As demand for functional foods increases, it is important to optimize production methods to preserve these health-promoting properties. This study investigated how various parameters influence the polyphenol content and antioxidant capacity of blackthorn tinctures. In the first stage, five ethanol concentrations (40–80% v/v) were tested, with 80% proving most effective for polyphenol extraction. In the second stage, the influence of fruit pre-treatment (blanched and pricked), sugar addition timing, and maceration duration (28–84 days) was assessed using tinctures made with 80% ethanol. Maximum total polyphenol content was recorded on day 84 in the variant with whole unprocessed fruits, and sugar added at the beginning. Phenolic acids, especially neochlorogenic acid, dominated the extracts. Anthocyanin concentrations reached their maximum during the early phase of maceration and subsequently exhibited a progressive decline over time, likely due to their inherent chemical instability under extraction conditions. Flavan-3-ols and phenolic acids remained more stable, particularly when sugar was added at the beginning. Antioxidant activity strongly correlated with polyphenol content and was highest in samples rich in polymeric procyanidins. Full article

Wild edible plants, historically valued for their medicinal properties, can be a sustainable source of food, cosmetics, and pharmaceuticals. The blue berries of Prunus spinosa L., known as blackthorns, have antioxidant, astringent, and antimicrobial benefits. To preserve these properties after harvesting, understanding the best storage methods is essential. In this study, blackthorns were preserved using different methods (air-drying, freezing, or freeze-drying) to determine the optimal procedure for preserving their antioxidant activity. The fruits were extracted using a 50:50 (V/V) mixture of ethanol and water. The different extracts were phytochemically characterized for their phenolic content and antioxidant activity. The Folin–Ciocalteu test revealed total phenolic contents of 7.97 ± 0.04, 13.99 ± 0.04, and 7.39 ± 0.08 (mg GAE/g raw material) for the three types of extracts, respectively. The total flavonoid contents were 2.42 ± 0.16, 3.14 ± 0.15, and 2.32 ± 0.03 (mg QE/g raw material), respectively. In line with the polyphenol analysis, the antioxidant activity as determined by DPPH method was higher for the frozen extract, with a value of 91.78 ± 0.80%, which was confirmed by the ROS test on keratinocytes. These results show that both air-drying and freeze-drying processes negatively impact the preservation of antioxidant activity in blackthorns, suggesting that freezing may be the best preservation method before bioactive compound extraction. Full article

Crataegus monogyna Jacq. and Prunus spinosa L. are common spring-flowering species in Slovenia. They occur in large stands and sometimes overgrow in unmanaged meadows. They are known as an excellent source of nectar for bees and other pollinators. Phenolic compounds are known as antioxidant for both pollinators and plants. We were interested in comparing plant species in terms of their phenolic compound content: rutin, quercetin, (iso)quercitrin, chlorogenic acid, and hyperoside. Their nectar was obtained from both plant species in 2024 in Ljubljana and the area surrounding Ljubljana. We took 96 samples of each species. The nectar was sampled with microcapillary tubes and analysed by HPLC. When studying the influence of abiotic factors on the concentration of phenolic compounds, the correlations were weak, so we cannot say with certainty which environmental factors affect which phenolic compounds and in what way. Rutin is predominant in the nectar of P. spinosa and chlorogenic acid is predominant in the nectar of C. monogyna. Hyperoside is found in the lowest concentration in both plant species. We found that although C. monogyna secreted much less nectar at midday, it was more concentrated in phenolic compounds at this time than in the morning. In P. spinosa, nectar secretion was highest in the morning, and the concentration of phenolic compounds was also highest in the morning. Full article

Plants have historically served as key sources of nutrition and popular medicine, which persists in current applications. The increasing demand for natural bioactive compounds has intensified the incorporation of plant-derived ingredients in both the food and pharmaceutical industries. This narrative review focuses on the fruits of Crataegus monogyna Jacq., Sorbus aria (L.) Crantz, and Prunus spinosa L. (Rosaceae), traditionally utilized in Europe and characterized by a high content of phenolic compounds, flavonoids, and anthocyanins. These metabolites are associated with antioxidant, anti-inflammatory, and cardioprotective properties. The available literature on their phytochemical profiles, biological activities, and integration into the Mediterranean Diet is critically assessed. Evidence supports their potential as functional food components. Despite encouraging in vitro results, the scarcity of in vivo and clinical studies limits the translational potential of these findings. Further research is warranted to validate their efficacy and safety in human health. This review underscores the value of integrating traditional ethnobotanical and ethnopharmacological knowledge with contemporary scientific research to explore novel applications of these underutilized wild fruits. Full article

Wild fruits are distributed worldwide, but are consumed mainly in developing countries, where they are an important part of the diet. Still, in many other countries, they are consumed only locally. Blackthorn (Prunus spinosa L.) is an underutilized species rich in fibres and phenolic compounds, making it suitable as a potential functional food for supporting human health. Cold (Cw) and hot (Hw) water-extracted (poly)phenolic polysaccharide–protein complexes, differing in carbohydrate, phenolic and protein contents, were isolated from blackthorn fruits and characterized. The complexes exhibited molecular weights of 235,200 g/mol (Cw) and 218,400 g/mol (Hw), and were rich in pectic polymers containing galacturonic acid, arabinose, galactose and rhamnose, indicating a dominance of homogalacturonan (HG) [→4)-α-D-GalA(1→4)-α-D-GalA(1→]n and a low content of RGI [→2)-α-L-Rha(1→4)-α-D-GalA(1→2)-α-L-Rha(1→]n sequences associated with arabinan or arabinogalactan. Minor content of glucan, probably starch-derived, was also solubilized. Pectic polysaccharides were highly esterified and partly acetylated. Pharmacological testing was performed in male Dunkin–Hartley guinea pigs, a model with human-like airway reflexes. Both complexes affected airway defense mechanisms. Particularly, Hw significantly suppressed citric acid-induced cough, similar to codeine, and reduced bronchoconstriction comparably to salbutamol in a dose-dependent manner. These findings support further exploration of Hw as a natural antitussive and bronchodilatory agent. Full article

Selecting factors that significantly affect the extraction process and optimizing them are essential to obtain high extraction efficiency. This study aimed at optimizing the ultrasonic-assisted extraction (UAE) of polyphenols from Romanian blackthorn fruits using aqueous solutions of ethanol as green extraction solvents. Six process factors, including solvent/plant material ratio (R_LS = 4.95–15.1 cm³/g), ethanol concentration in the extraction solvent (c_et = 16.4–83.6%), extraction temperature (t = 30–70 °C), pH of the solvent (pH = 2–7), amplitude of the ultrasonic probe (A = 30–70%), and extraction time (τ = 5–15 min), were screened and optimized based on a Plackett–Burman design (PBD) and a central composite design (CCD). Statistical analysis indicated that R_LS, c_et, and t significantly affected the process response variables in terms of total phenolic content (TPC), total anthocyanin content (TAC), and antioxidant capacity (AC). Under optimal conditions (R_LS,opt = 15.1 cm³/g, c_et,opt = 33.2%, t_opt = 66.8 °C, pH_opt = 7, A_opt = 50%, and τ_opt = 10 min), the following levels of response variables were experimentally determined: TPC_opt = 14.45 ± 0.718 mg GAE/g DM, TAC_opt = 0.405 ± 0.057 mg C3GE/g DM, and AC_opt = 16.75 ± 1.144 mg TE/g DM. Six phenolic compounds were identified in the extract obtained at optimal levels of process factors, i.e., rutin (7.12 ± 0.06 mg/100 g DM), protocatechuic acid (6.83 ± 0.01 mg/100 g DM), neochlorogenic acid (4.88 ± 0.01 mg/100 g DM), vanillic acid (3.70 ± 0.01 mg/100 g DM), chlorogenic acid (1.93 ± 0.02 mg/100 g DM), and caffeic acid (1.51 ± 0.01 mg/100 g DM). Full article

Due to their antioxidant and other beneficial properties, polyphenol-rich plants are important functional foods. The purpose of the study was to evaluate the content of two polyphenols—ellagic acid and quercetin—in fruits of wild medicinal plants of Polish origin, as potential sources of these compounds. The research material was chosen considering both the popularity of the fruits and their recognized medicinal and pharmaceutical properties. All selected fruits—barberry (Berberis vulgaris), blackthorn (Prunus spinosa), chokeberry (Aronia melanocarpa), elderberry (Sambucus nigra), hawthorn (Crataegus monogyna), lingonberry (Vaccinium vitis-idaea), rowanberry (Sorbus aucuparia), and sea-buckthorn (Hippophae rhamnoides syn. Elaeagnus rhamnoides)—are known for therapeutic use in Polish folk medicine. Extracts were analyzed for ellagic acid and quercetin content using UHPLC-ESI-MS/MS. Quantitative results revealed that barberries, blackthorn, and sea-buckthorn contained the highest amounts of ellagic acid, up to 3.29 ± 0.24, 3.50 ± 0.16, and 4.80 ± 0.18 μg/g dw, respectively, while lingonberry provided up to 196.20 ± 3.10 μg/g dw of quercetin, making it a valuable dietary source of this flavonoid. The study confirms that Polish wild medicinal plants are valuable reservoirs of key polyphenols relevant to human health and support their potential inclusion in dietary strategies for disease prevention. Full article

This study investigates the potential reuse of Prunus spinosa (blackthorn) seeds, a food industry by-product. Traditionally discarded, these seeds are now being explored for their bioactive compounds. In this work, seeds were used as raw material for supercritical CO₂ extraction. Two distinct extracts were obtained at low and high pressure (SFE90 and SFE200) and both extracts presented an aqueous phase (WE90 and WE200). SFE90 analysis by GC/MS allowed us to identify benzaldehyde and fatty acids (mainly oleic and linoleic acids). The fatty acid profile of SFE200, determined by HPLC-DAD/ELSD, showed that oleic and linoleic acids were predominant in supercritical oil. The phytochemical composition of the water extracts, analyzed via LC-DAD-ESI-MS, revealed that higher pressure enhanced the recovery of specific flavonols and anthocyanins, while lower pressure preserved various polyphenolic subclasses. WE90 was rich in 3-feruloylquinic acid and cyanidin-3-O-rutinoside, whereas WE200 was rich in caffeic acid hexoside 2 and dihydro-o-coumaric acid glucoside. Benzaldehyde was individuated in WE90 and WE200 by HPLC-DAD analysis. Cytotoxicity assays demonstrated that WE90, WE200 and SFE200 had anticancer effects on SH-SY5Y neuroblastoma cells, while all extracts did not remarkably affect the viability and morphology of human skin keratinocytes (HaCaT cells). These results suggest that P. spinosa seed extracts have potential nutraceutical and pharmaceutical applications. Full article

Understanding how woody plants cope with severe water shortages is critical, especially for regions where droughts are becoming more frequent and intense. We studied the effects of drought intensity, focusing on post-drought resprouting, autumn leaf senescence and the subsequent spring bud burst. Furthermore, we aimed to study population differentiation in the drought and post-drought responses. We performed a summer dry-out experiment in a common garden of potted Prunus spinosa L. (Rosaceae) saplings. We analysed responses across different visual stress symptom categories and examined differentiation between provenances from a local origin (Western Europe, Belgium), a lower latitude (Spain) and a higher latitude (Sweden). The chance of post-drought resprouting was greater for the more severely affected plants than for the less severely affected ones, and it occurred earlier. The plants that displayed wilting of the leaves during the drought had a leaf senescence 2.7 days earlier than the controls, whereas that of plants with 25 to 75% and more than 75% of desiccated leaves was 7 and 15 days later, respectively. During the drought, the local provenance was the first to develop visual symptoms compared to the other two provenances. However, among plants that exhibited no or only mild symptoms, this provenance also had a higher likelihood of post-drought resprouting. Among the control plants, the higher-latitude provenance displayed leaf senescence earlier, while the lower-latitude provenance senesced later compared to the local provenance. However, these differences in the timing of leaf senescence among the three provenances disappeared in treated plants with more than 25% of desiccated leaves due to the drought. Whereas leaf senescence could be earlier or later depending on the developed drought symptoms, the timing of bud burst was only delayed. Results indicate that resprouting and timing of leaf senescence are responsive to the severity of the experienced drought in a provenance-dependent way. Full article