Search Results (767)

Cacao genotypes propagation through plant tissue culture represents a strategic approach for establishing a core collection of elite plants to be used as a donor material source, necessary for increasing new planting areas of cacao. This study aimed to evaluate somatic embryo regeneration in ten native fine-aroma cacao genotypes (INDES-06, INDES-11, INDES-14, INDES-32, INDES-52, INDES-53, INDES-63, INDES-64, INDES-66, INDES-70) from the INDES-CES germplasm collection, under in vitro conditions using culture medium supplemented with different concentrations of Thidiazuron (0, 10, and 20 nM). Our results showed an average of 20 and 100% of callogenesis in all genotypes evaluated, but the callus development did not appear after early stages of its induction; however, primary somatic embryos were observed after 42 days after TDZ treatment in the INDES-52, INDES-53, INDES-64, INDES-66, INDES-70 genotypes. The INDES-52 genotype was more responsive to under 20 nM of TDZ, generating an average of 17 embryos per explant. This study contributes to the adaptation and establishment of a protocol for somatic embryo regeneration of fine-flavor cacao genotypes. Full article

Soil acidity is one of the main limiting factors for coffee production in Peruvian rainforests. The objective of this study is to predict the spatial acidity variability for recommending site-specific liming and phosphorus fertilization treatments. We analyzed thirty-six edaphoclimatic variables, eight methods for estimating liming doses, and three geospatial variables from 552 soil samples in the Pichanaqui district of Peru. Multivariate statistics, nonparametric comparison, and geostatistical analysis with Ordinary Kriging interpolation were used for data analysis. The results showed low coffee yields (0.70 ± 0.16 t ha⁻¹) due to soil acidification. The interquartile ranges (IQR) were found to be 3.80–5.10 for pH, 0.21–0.87 cmol Kg⁻¹ for Al⁺³, and 2.55–6.53 mg Kg⁻¹ for available P, which are limiting soil conditions for coffee plantations. Moreover, pH, Al⁺³, Ca⁺², and organic matter (OM) were the variables with the highest accuracy and quality in the spatial prediction of soil acidity (R² between 0.77 and 0.85). The estimation method of liming requirements, MPM (integration of pH and organic material method), obtained the highest correlation with soil acidity-modulating variables and had a high spatial predictability (R² = 0.79), estimating doses between 1.50 and 3.01 t ha⁻¹ in soils with organic matter (OM) > 4.00%. The MAC (potential acidity method) method (R² = 0.59) estimated liming doses between 0.51 and 0.88 t ha⁻¹ in soils with OM < 4.00% and potential acidity greater than 0.71 cmol Kg⁻¹. Regarding phosphorus fertilization (DAP), the results showed high requirements (median = 137.21 kg ha⁻¹, IQR = 8.28 kg ha⁻¹), with high spatial predictability (R² = 0.74). However, coffee plantations on Ferralsols, with Paleogene parental material, mainly in dry forests, had the lowest predicted fertilization requirements (between 6.92 and 77.55 kg ha⁻¹ of DAP). This research shows a moderate spatial variation of acidity, the need to optimize phosphorus fertilization, and an optimal prediction of liming requirements using the MPM and MAC methods, which indicate high requirements in the southwest of the Pichanaqui district. Full article

Background/Objectives: Loneliness is the sensation of feeling alone or emotionally isolated, even when one is surrounded by other people. It is associated with conditions such as depression, anxiety, harmful habits, and cardiovascular problems. The main objective of the present study was to develop and determine the psychometric properties of the Multidimensional Loneliness Scale (MLS), which is a self-report instrument. Methods: The present study is instrumental in nature, as it aims to analyze the psychometric properties of a new assessment instrument. A total of 484 adults, both men and women, aged between 18 and 55 years, participated in this research. Results: Exploratory factor analysis revealed the presence of four dimensions: social disconnection, family estrangement, loss of attachment figure, and intrapersonal emptiness. Confirmatory factor analysis demonstrated that the four-dimensional model exhibited a good fit (CFI = 0.91; RMSEA = 0.07; SRMR = 0.07; AIC = 737.87). The concurrent validity was evidenced by significant correlations with the De Jong Gierveld scale and the Revised UCLA Loneliness Scale. The reliability analysis demonstrated satisfactory internal consistency, with omega coefficients ranging from 0.84 to 0.92 and alpha coefficients from 0.84 to 0.93. Conclusions: The MLS is a self-report instrument designed to assess loneliness, and it has satisfactory psychometric properties. Full article

Creole goats have adapted to the harsh Andean environment, yet the physiological impacts of high-altitude production systems remain underexplored. This study assessed seasonal and dietary influences on the hematological and biochemical profiles of 45 Creole goats in the Peruvian Andes. The animals were assigned to three diets: D1 (grazing), D2 (grazing + 2000 g hay), and D3 (grazing + 400 g concentrate), across rainy and dry seasons. Biweekly blood sampling measured urea, cholesterol, total protein, albumin, ALP, ALT, WBCL, NeuP, LymP, HGB, and MCV. Season exerted the strongest influence (p < 0.001), with modest dietary effects and a consistent effect of sampling time. Urea, total protein, and albumin increased during the rainy season, though only urea responded significantly to diet. Leukocytosis rose in the dry season and with higher-protein diets, suggesting heightened immune activation under environmental stress. Hemoglobin peaked in the rainy season and early sampling, indicating better oxygenation. MCV and body weight were higher in the dry season, with weight unaffected by diet. These results underscore the complex interplay of environmental and nutritional factors in shaping goat physiology at high altitudes, emphasizing the importance of dynamic modeling in sustainable Andean livestock systems. Full article

The high-Andean grass Jarava ichu (Poaceae) plays a vital role in water regulation and aquifer recharge. However, its limited use is often linked to forest fires, highlighting the need for sustainable alternatives. Therefore, this study aims to explore the valorization of ichu as a substrate for the cultivation of Pleurotus spp. (P. citrinopileatus, P. djamor, and P. ostreatus) and to evaluate the potential of the residual substrate as a biofertilizer, offering an ecological alternative to grassland burning in the Peruvian Andes. Samples of ichu from the district of Tomás (Lima, Peru) were used as culture substrate, analyzing productivity indicators such as crop cycle (CC), biological efficiency (BE), and production rate (PR), together with the nutritional profile of the fungi and the chemical properties of the residual substrate. The results showed an average biological efficiency of 19.8%, with no significant differences (p > 0.05) in CC, BE, or PR among the species, confirming the viability of ichu as a substrate. The fungi presented a high protein content (24.1–30.41% on a dry basis), highlighting its nutritional value. In addition, the residual substrate exhibited elevated levels of phosphorus (795.9–1296.9 ppm) and potassium (253.1–291.3 ppm) compared to raw ichu (0.11–7.77 ppm for both nutrients). Germination tests on radish seeds showed rates between 80% and 100%, without inhibition, supporting its potential as a biofertilizer. This study demonstrates the double potential of ichu as a substrate for the sustainable production of edible mushrooms of high nutritional value and as a source of biofertilizers. Full article

Non-Newtonian debris flows represent a critical challenge for hydraulic infrastructure in mountainous regions, often causing significant damage and service disruption. However, current models typically simplify these flows as Newtonian, leading to inaccurate design assumptions. This study addresses this gap by comparing the hydraulic behavior of Newtonian and non-Newtonian flows in an alluvial fan, using the Amoray Gully in Apurímac, Peru, as a case study. This gully intersects the Interoceánica Sur national highway via a low-water crossing (baden), making it a relevant site for evaluating debris flow impacts on critical road infrastructure. The methodology integrates hydrological analysis, rheological characterization, and hydraulic modeling. QGIS 3.16 was used for watershed delineation and extraction of physiographic parameters, while a high-resolution topographic survey was conducted using an RTK drone. Rainfall-runoff modeling was performed in HEC-HMS 4.7 using 25 years of precipitation data, and hydraulic simulations were executed in HEC-RAS 6.6, incorporating rheological parameters and calibrated with the footprint of a historical event (5-year return period). Results show that traditional Newtonian models underestimate flow depth by 17% and overestimate velocity by 54%, primarily due to unaccounted particle-collision effects. Based on these findings, a multi-barrel circular culvert was designed to improve debris flow management. This study provides a replicable modeling framework for debris-prone watersheds and contributes to improving design standards in complex terrain. The proposed methodology and findings offer practical guidance for hydraulic design in mountainous terrain affected by debris flows, especially where infrastructure intersects active alluvial fans. Full article

(1) Background: The Peruvian healthcare system is widely regarded as deficient, with ongoing improvements identified as a key area of need. This study sought to assess user satisfaction and the quality of care in two public hospitals in Trujillo. (2) Methods: A non-experimental, cross-sectional, and correlational study was carried out. A group of 384 people who used two public hospitals in the city of Trujillo was studied. The people in the study were chosen based on the researchers’ convenience sampling. Information was collected using a survey based on the SERVQUAL model. This survey was used to evaluate the quality of service. Descriptive and inferential analyses were performed, including Spearman’s correlation and multinomial logistic regression to assess associations and identify key predictors of perceived service quality. (3) Results: The results indicated that 97.66% of the users perceived a low quality of care and 100% expressed dissatisfaction with the services. The most critical dimensions were reliability and responsiveness, while tangible items obtained better results. A positive correlation (rho = 0.723) was identified between quality of care and user satisfaction, with empathy (rho = 0.559) and safety (rho = 0.543) emerging as the most influential dimensions. (4) Conclusions: Responsiveness and Security were identified as key predictors of the perceived service quality in two public hospitals in Trujillo, Peru. Despite high empathy correlations, only timely care and safety significantly influenced satisfaction. The findings align with SDG 3, calling for improved efficiency and humanized care in public health services. Full article

This study introduces and validates a novel instrument to measure aggressive gender backlash (AGB), a distinct and underexplored dimension of gender backlash (GB) within intimate partner relationships. Based on the General Aggression Model, a multidimensional scale was developed and tested using data from 513 Peruvian female microentrepreneurs. Results demonstrate solid evidence of reliability, discriminant validity, and predictive validity across five dimensions: hostility, the withdrawal of support, sabotage/coercion, gender stereotyping, and masculine victimization. The findings reveal that AGB is more prevalent than intimate partner violence against women (IPVAW) and often precedes it. AGB encompasses covert, non-violent behaviors that aim to resist female empowerment, such as emotional sabotage, manipulation, and disqualification, often normalized within relationships. This construct is significantly associated with lower levels of empowerment, increased subordination, emotional morbidity, and decreased work productivity. This study redefines GB as an interpersonal process measurable at the individual level and provides the first validated tool for its assessment. By conceptualizing AGB as a persistent, harmful, and functionally equivalent mechanism to IPVAW, though not necessarily physically violent, this research fills a key gap in gender violence literature. It offers practical implications for early detection and prevention strategies. Full article

According to the literature, Schinus molle (SM) is an important source of bioactive phytochemicals, but the phytochemical content and composition of this species, which grows in high Andean geographic zones such as Tarma (Peru), is not known. In an effort to fill this gap, our work investigated the supercritical carbon dioxide extraction of SM leaves at three temperature levels (35, 45, and 55 °C) and three pressure levels (150, 250, and 350 bar). The results revealed the highest yield of extract at 150 bar, 45 °C, and 3.28 g CO₂/min. Under these conditions, the overall extraction curves (OEC) were modeled using the Spline, logistic, and Esquível models, allowing the generation of mass transfer parameters for SFE at the optimized conditions, resulting in a similar correlation with experimental data. Twenty-six compounds were identified in the SFE extract of SM leaves. The most abundant compound classes were sesquiterpenoids (57.17%), sesquiterpenes (24.50%), and triterpenoids (10.48%); of each class, the most abundant compounds were shyobunol (33.60%), bicyclogermacrene (12.68%), and lupeone (6.58%), respectively. The compounds detected possess bioactive properties that support further studies on the application of SFE extracts of SM as a functional ingredient in commercial products. Full article

Introduction: Work life quality (WLQ) in nursing is a critical factor that influences both staff well-being and the quality of care provided to patients. Spiritual intelligence (SI) and servant leadership (SL) have been identified as potential positive predictors of WLQ, as they facilitate resilience, job satisfaction, and stress management in highly demanding hospital environments. However, the specific relationship between these constructs in the Peruvian nursing context has not yet been thoroughly explored. Objective: We aimed to examine the impact of spiritual intelligence and servant leadership on the work life quality of Peruvian nurses, assessing their predictive role through a structural equation modeling approach. Methods: A cross-sectional and explanatory study was conducted with a sample of 134 Peruvian nurses (M = 36.29 years, SD = 7.3). Validated Spanish-language instruments were used to measure SI, SL, and WLQ. Structural equation modeling (SEM) with a robust maximum likelihood estimator (MLR) was employed to evaluate the relationships between the variables. Results: Spiritual intelligence showed a positive correlation with WLQ (r = 0.40, p < 0.01) and with servant leadership (r = 0.44, p < 0.01). Likewise, servant leadership had a significant relationship with WLQ (r = 0.53, p < 0.01). The structural model demonstrated a good fit (χ² = 1314.240, df = 970, CFI = 0.96, TLI = 0.96, RMSEA = 0.05, SRMR = 0.08). The hypothesis that SI positively predicts WLQ was confirmed (β = 0.41, p < 0.001), as was the significant effect of SL on WLQ (β = 0.26, p < 0.001). Conclusions: The results indicate that both spiritual intelligence and servant leadership are key predictors of work life quality in Peruvian nurses. SI contributes to developing a transcendent perspective on work and greater resilience, while SL fosters a positive and motivating organizational environment. It is recommended to implement training programs and leadership strategies focused on these constructs to enhance work life quality in the healthcare sector. Full article

Adobe churches are representative of Andean architectural heritage, yet their structural vulnerability to seismic events remains a significant concern. This study evaluates the seismic performance of the 17th-century Church of Santo Tomás de Aquino in Rondocan, Peru, an adobe building that underwent conservation work in the late 1990s. The assessment combines in situ inspections and experimental testing with advanced nonlinear numerical modeling. A finite-element macro-model was developed and calibrated using sonic and ambient vibration tests to replicate the observed structural behavior. Nonlinear static (pushover) analyses were performed in the four principal directions to identify failure mechanisms and to evaluate seismic capacity using the Peruvian seismic code. Kinematic limit analyses were conducted to assess out-of-plane mechanisms using force- and displacement-based criteria. The results revealed critical vulnerabilities in the rear façade and lateral walls, particularly in terms of out-of-plane collapse, while the main façade exhibited a higher capacity but a brittle failure mode. This study illustrates the value of advanced numerical simulations, calibrated with field data, as effective tools for assessing seismic vulnerability in historic adobe buildings. The outcomes highlight the necessity of strengthening measures to balance life safety requirements with preservation goals. Full article

Background/Objectives: Aneurysmal subarachnoid hemorrhage (aSAH) is a medical emergency with a high mortality rate requiring urgent treatment. This study aimed to identify clinical predictors of inpatient mortality and poor postoperative course after aSAH surgical clipping. Methods: We performed a retrospective review of medical records for 210 patients with aSAH treated via surgical clipping at our institution between 2010 and 2019. Baseline demographic data and clinical characteristics related to aSAH were collected. To identify factors associated with inpatient mortality and a poor postoperative course after aSAH microsurgical clipping, we conducted a univariate and bivariate analysis, as well as a multivariate analysis via the Poisson regression model. Results: The overall cumulative mortality over the 10-year study period was 11.43%. A severe WFNS scale score (aRR: 2.86; 95% CI: 1.28–6.39; p = 0.011) and having 1 (aRR: 5.76; 95% CI: 2.02–16.39, p = 0.001) or ≥2 (aRR: 18.86; 95% CI: 5.16–68.90, p < 0.001) postoperative neurosurgical complications were associated with an increased risk of inpatient mortality. A moderate (aRR: 3.71; 95% CI: 1.45–9.50; p = 0.006) or severe (aRR: 4.18; 95% CI: 1.12–15.60; p = 0.034) Glasgow scale score on admission, and presenting 1 (aRR: 2.31; 95% CI: 1.27–4.19; p = 0.006) or ≥2 postoperative clinical complications (aRR: 3.34; 95% CI: 1.83–6.10; p < 0.001) were associated with an increased risk of a poor postoperative course. Conclusions: While promising and widely supported by the published literature, these findings require further validation in a larger prospective and multi-centered study to adequately propose health policies on neurointensive care for the Peruvian population. Ultimately, developing socioeconomic setting-focused intervention algorithms and clinical practice guidelines could enhance the survival and postoperative course of patients presenting with aSAH. Full article

Aloysia triphylla is widely used in traditional medicine from Peru for its sedative, digestive and anti-inflammatory properties. However, comprehensive studies on the biological activities of its essential oil (EO), particularly from Peruvian sources, remain limited. This study aimed to analyze the chemical composition and enantiomeric profile of A. triphylla EO and evaluate its antibacterial, antioxidant, anticholinesterase, and cytotoxic activities. The EO was obtained by steam distillation and analyzed using gas chromatography–mass spectrometry (GC-MS). A total of 62 compounds were identified, with (E)-caryophyllene (16.80%), β-pinene (9.96%), and germacrene D (10.00%) being the major components. Enantiomeric analysis revealed specific chiral signatures, including (−)-α-pinene, (+)-limonene, and (R)-(−)-linalool. The EO exhibited significant antibacterial activity, particularly against Bacillus subtilis (MIC = 5 µg/mL), and weak antioxidant activity (IC₅₀ = 7720 and 4648 µg/mL for DPPH and ABTS, respectively). Additionally, the EO demonstrated moderate acetylcholinesterase inhibition (IC₅₀ = 87.8 µg/mL) and cytotoxicity in the Artemia salina assay (LC₅₀ = 964 µg/mL). These findings suggest that A. triphylla EO possesses promising bioactivities with potential applications in pharmaceutical and cosmetic fields. Full article

Allergic diseases, particularly respiratory allergies like asthma and allergic rhinitis, are a growing public health concern influenced by environmental factors such as climate change and air pollution. The exposome framework enables a comprehensive assessment of how lifelong environmental exposures shape immune responses and allergic sensitization. Peru’s diverse ecosystems and climates provide a unique setting to investigate regional variations in allergic sensitization. This study characterized these patterns in five Peruvian regions with distinct climatic, urbanization, and socioeconomic characteristics. A total of 268 individuals from Lima, Piura, Tarapoto, Arequipa, and Tacna were analysed for allergen-specific IgE responses using a multiplex IgE detection system. The results revealed significant geographical differences in sensitization frequencies and serodominance profiles, based on descriptive statistics and supported by Chi-square comparative analysis. House dust mites were predominant in humid regions, while Arequipa exhibited higher sensitization to cat allergens. In Tacna, olive pollen showed notable prevalence alongside house dust mites. Tarapoto’s high humidity correlated with increased fungal and cockroach allergen sensitization. Notably, some allergens traditionally considered minor, such as Der p 5 and Der p 21, reached sensitization prevalences close to or exceeding 50% in certain regions. These findings provide the most detailed molecular characterization of allergic sensitization in Peru to date, highlighting the importance of region-specific allergy management strategies. Understanding environmental influences on allergic diseases can support more effective diagnostic, therapeutic, and preventive approaches tailored to diverse geographical contexts. Full article