Search Results (6,975)

Background: The typification of the name Aegilops tauschii Coss. (Poaceae: Triticeae) is revisited. Several authors cited a gathering from Iberia as the locality and Buxbaum as the collector of as the type, but no actual specimens from this collection have been located, nor is there evidence that such a gathering existed. In 1994, van Slageren designated as lectotype an illustration from Buxbaum’s Plantarum minus cognitarum centuria I (1728), which, although original material, is not the only element cited in the protologue. The protologue mentions several gatherings, some of which are represented by identifiable herbarium specimens qualifying as syntypes. Methods: This work is based on the analysis of the protologue of Aegilops tauschii and the study of specimens conserved in several herbaria. According to the International Code of Nomenclature for algae, fungi, and plants (ICN, Shenzhen Code 2018), an illustration does not hold the same nomenclatural weight as preserved specimens cited in the protologue. Therefore, van Slageren’s lectotypification does not comply with Art. 9.12 of the ICN and must be superseded. Results: The original material includes multiple elements, and a new lectotype is designated from a specimen at PRC from Azerbaijan. Full article

DFT calculations at the B3LYP/aug-cc-pVDZ level of theory on some aromatic substrates showed that in the HOMO (Highest Occupied Molecular Orbital) of nitrobenzene, the atomic coefficients are not in agreement with the meta-directing behavior of this compound. The atomic coefficients are the same in the ortho and in the meta positions. The HOMO (or NHOMO (Next Occupied Molecular Orbital) in the case of benzaldehyde) is not in agreement with the experimental results when deactivating, meta-orienting compounds are considered. Mulliken charges sometimes are not able to explain the observed reactivity. Hirshfeld charges allow us to predict the orientation of the attack of an electrophile on the aromatic ring, with the exception of nitrobenzene. Both HOMO atomic coefficients and charges are in agreement with the experimental results when deactivating, ortho-para orienting, and activating compounds are tested. Full article

Cacao genotypes propagation through plant tissue culture represents a strategic approach for establishing a core collection of elite plants to be used as a donor material source, necessary for increasing new planting areas of cacao. This study aimed to evaluate somatic embryo regeneration in ten native fine-aroma cacao genotypes (INDES-06, INDES-11, INDES-14, INDES-32, INDES-52, INDES-53, INDES-63, INDES-64, INDES-66, INDES-70) from the INDES-CES germplasm collection, under in vitro conditions using culture medium supplemented with different concentrations of Thidiazuron (0, 10, and 20 nM). Our results showed an average of 20 and 100% of callogenesis in all genotypes evaluated, but the callus development did not appear after early stages of its induction; however, primary somatic embryos were observed after 42 days after TDZ treatment in the INDES-52, INDES-53, INDES-64, INDES-66, INDES-70 genotypes. The INDES-52 genotype was more responsive to under 20 nM of TDZ, generating an average of 17 embryos per explant. This study contributes to the adaptation and establishment of a protocol for somatic embryo regeneration of fine-flavor cacao genotypes. Full article

Background and Objectives: The causes and clinical outcomes of renal perfusion abnormalities occurring after para-aortic lymphadenectomy (PANDx) for gynecologic malignancies are unknown. We investigated the potential involvement of accessory renal artery (ARA) obstruction in their development by reassessing perioperative contrast-enhanced computed tomography (CECT). Materials and Methods: This retrospective study investigated a clinical database to identify urinary contrast defects using CECT in all patients who had undergone PANDx between January 2020 and December 2024. The perfusion defects in the kidney detected by CECT were extracted by a gynecologic oncologist and evaluated by a radiologist and urologist for suspected obstruction of ARAs. Results: Postoperative renal contrast defects were observed in 3.8% (6/157) of patients. Renal parenchymal fibrosis, cortical atrophy, and parenchymal thinning were observed as universal findings in all patients showing renal contrast defects. In five of the six cases, ARAs supplying the infarcted renal segments were identified on preoperative CECT, and arterial obstruction was confirmed on postoperative imaging. The remaining case was considered to be latent pyelonephritis. All five patients underwent laparotomy, and preoperative CECT failed to detect ARAs. The median resected para-aortic lymph node was 23 nodes (range: 15–33) in five patients, showing no statistically significant difference compared to patients without perfusion abnormalities (p = 0.19). Postoperative serum creatinine levels remained stable. Conclusions: ARA obstruction appears to be a risk factor for segmental renal infarction after para-aortic lymphadenectomy in gynecological malignancies; however, the clinical impact on urinary function may be limited. Awareness of this potential complication is essential for gynecologic oncologists performing PANDx. Full article

The taxonomy of Laccaria laccata, the type species of the genus Laccaria, has long been ambiguous due to the absence of a reference sequence and the reliance on early, morphology-based descriptions. To resolve this issue, we selected a Code-compliant lectotype for Agaricus laccatus—the basionym of L. laccata—from Schaeffer’s 1762 illustration cited in Fries’ sanctioning work. Given the limitations of this historical material for modern species interpretation, we also designated an epitype based on Singer’s collection C4083 (BAFC) from Femsjö, Sweden, which was previously but not effectively designated as the “lectotype” by Singer. This epitype is supported by detailed morphological descriptions, iconography, and newly generated nrITS, nrLSU, and RPB2 sequences, which have also been newly obtained from additional collections. Phylogenetic analyses consistently place the epitype of L. laccaria within a well-supported clade, herein designated as/Laccaria laccata, which includes sequences previously reported as falling within the “proxima 1 clade”. This integrative approach, combining historical typification with modern molecular and morphological data, stabilizes the nomenclature of L. laccata and provides a robust foundation for future studies of this ecologically and economically important genus of ectomycorrhizal fungi. Full article

Ceramic pastes used in additive manufacturing offer several advantages, including low production costs due to the availability of raw materials and efficient processing methods, as well as a reduced environmental footprint through minimized material waste, optimized resource use, and the inclusion of recyclable or sustainably sourced components. This study evaluates the effect of diatomite content in a ceramic paste composed of carboxymethyl cellulose, kaolinite, and feldspar on its extrusion behavior and thermal conductivity, with additional analysis of its implications for microstructure, mechanical properties, and thermal performance. Four ceramic pastes were prepared with diatomite additions of 0, 10, 30, and 60% by weight. Thermal conductivity, extrusion behavior, morphology, and distribution were examined using scanning electron microscopy (SEM), while thermal degradation was assessed through thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). The results show that increasing diatomite content leads to a reduction in thermal conductivity, which ranged from 0.719 W/(m·°C) for the control sample to 0.515 W/(m·°C) for the 60% diatomite sample, as well as an improvement in extrusion behavior. The ceramic paste demonstrated adequate extrusion performance for 3D printing at diatomite contents above 30%. These findings lay the groundwork for future research and optimization in the development of functional ceramic pastes for advanced manufacturing applications. Full article

Sustainable education requires addressing the challenges posed by digital transformation, including technostress among university students. This study evaluates technostress levels in higher education through the validation of the TS4US scale and its implications for sustainable learning environments. A cross-sectional study was conducted with 328 university students from four districts in Lima, Peru, using an online survey to measure technostress. Confirmatory factor analysis (CFA) was performed to assess the psychometric properties of the TS4US scale, resulting in a refined model with two latent factors and thirteen validated items. Findings indicate that 28% of students experience high technostress levels, while 5% report very high levels, though no significant associations were found between technostress and sociodemographic variables such as campus location, employment status, gender, and academic level. The TS4US instrument had been previously validated in Chile; this study confirms its structure in a new sociocultural context, reinforcing its cross-cultural applicability. These results highlight the need for sustainable strategies to mitigate technostress in higher education, including institutional support, digital literacy programs, and policies fostering a balanced technological environment. Addressing technostress is essential for promoting sustainable education (SDG4) and enhancing student well-being (SDG3). This study directly contributes to the achievement of Sustainable Development Goals 3 (Good Health and Well-being) and 4 (Quality Education) by providing validated tools and evidence-based recommendations to promote mental health and equitable access to digital education in Latin America. Future research should explore cross-country comparisons and targeted interventions, including digital well-being initiatives and adaptive learning strategies, to ensure a resilient and sustainable academic ecosystem. Full article

This work expands the processing window of the laser powder bed fusion (LPBF) processing of WE43 magnesium alloy by evaluating laser powers and scanning speeds up to 400 W and 1200 mm/s, and their effect on densification, microstructure, and electrochemical performance. Relative density of 99.9% was achieved for 300 W and 800 mm/s, showing that the use of high laser power is not a limitation for the manufacturing of Mg alloys, as has been usually considered. Microstructural characterisation revealed refined grains and the presence of RE-rich intermetallic particles, while microhardness increased with height due to thermal gradients. Electrochemical testing in 3.5 wt.% NaCl solution, a more aggressive media than those already used, indicated that the corrosion of samples with density values below 99% is conditioned by the porosity; however, above this value, in the WE43, the corrosion evolution is more related to the microstructure of the samples, according to electrochemical evaluation. This study demonstrates the viability of high-energy LPBF processing for WE43, offering optimised mechanical and corrosion properties for biomedical and structural applications. Full article

Surveillance of swine influenza A virus (swIAV) traditionally focuses on respiratory matrices, yet emerging evidence suggests that fecal shedding and secondary environmental contamination may also contribute to viral dissemination. In this study, we collected and analyzed nasal, rectal, environmental, milk, and colostrum samples from naturally infected pigs in a commercial farm in Minas Gerais, Brazil. IAV RNA was detected in 25% of samples, including 42% from asymptomatic animals, with nasal swabs showing higher detection rates (30%) than rectal swabs (20%), though rectal Ct values were consistently higher, indicative of lower viral loads. We successfully isolated viable viruses from feces and effluent samples. Whole-genome sequencing revealed co-circulation of enzootic pH1N1 clade #2 (HA) and pN1 clade #4 (NA), alongside human-origin H3N2 sequences clustering within clade 3C.2a1b.2a.2a.1, and N2 segments related to pre-3C human lineages from 2001 to 2002. Phylogenetic and p-distance analyses support both recent reverse zoonosis and historical transmission events. Detection of complete HA/NA sequences from rectal swabs and treated effluent further emphasizes the surveillance value of non-respiratory matrices. The integration of respiratory and fecal/environmental sampling appears important to achieve more comprehensive IAV monitoring in swine herds and may have significant implications for One Health strategies in Brazil and beyond. Full article

Human exploitation has been a major driver of marine turtle population declines, particularly affecting naturally scarce species such as the pantropical hawksbill turtle. Although hawksbill sea turtles have been documented in the Gulf of California since the early 20th century, data on their historical demography during periods of high exploitation in this region are nonexistent. We investigated the size structure of hawksbill turtles from the Western Central Gulf of California by examining a unique sample of decorative taxidermies, corresponding to 31 specimens captured during fishing operations near Santa Rosalía, Baja California Sur, Mexico, between 1980 and 1990. An analysis of the curved carapace measures revealed a length range (nuchal notch to posterior of supracaudals) of 29.5–59.5 cm (mean = 38.75 ± 6.67 cm) and a width range of 25.0–51.5 cm (mean = 33.63 ± 5.66 cm), with 87% of specimens having lengths between 30 and 45 cm. Based on the carapace length measurements, we estimated the ages to be between 7 and 20 years, indicating that the population included juveniles. Our findings provide baseline data for an understudied period and region, suggesting that this area previously served as an important juvenile habitat. These results contribute essential historical demographic information for conservation planning. Full article

This article presents a probabilistic methodology for assessing corrosion initiation in reinforced concrete structures exposed to chloride ingress. The approach addresses key limitations of conventional analytical models by accounting for non-diffusive barriers and incorporating a rigorous statistical treatment of censored data to mitigate biases introduced by limited simulation durations. A combination of analytical solutions for diffusion from opposite sides with time-dependent boundary conditions is also proposed and validated. The probabilistic study includes the depassivation assessment of a hollow pier section. The blocking effect caused by rebars is statistically characterised through correction factors derived from finite element simulations. These factors are used to adjust analytical solutions, which are computationally inexpensive. Results show that neglecting the rebar blocking effect can overestimate the mean corrosion initiation time by up to 42%, while the use of censored data reduces bias in lifetime estimates. The observed frequency of censored events reached up to 20% when simulations were truncated at 100 years. The corrected analytical models closely match the finite element results, statistically validating their application. The case study indicates premature corrosion initiation (less than 10 years to achieve target reliability), underscoring the need to better reconcile the desired levels of reliability with realistic input parameters for depassivation. Full article

Massively parallel or next-generation sequencing (NGS) has enabled the genetic characterization of cancer patients, allowing the identification of somatic and germline variants associated with their diagnosis, tumor classification, and therapy response. Despite its benefits, NGS testing is not yet available in the Chilean public health system, rendering it both costly and time-consuming for patients and clinicians. Using a retrospective cohort of 67 formalin-fixed, paraffin-embedded (FFPE) colorectal cancer (CRC) samples, we aimed to implement the identification, annotation, and prioritization of relevant actionable tumor somatic variants in our laboratory, as part of the public health system. We compared two different library preparation methodologies (amplicon-based and capture-based) and different bioinformatics pipelines for sequencing analysis to assess advantages and disadvantages of each one. We obtained 80.5% concordance between actionable variants detected in our analysis and those obtained in the Cancer Genomics Laboratory from the Universidad de Chile (62 out of 77 variants), a validated laboratory for this methodology. Notably, 98.4% (61 out of 62) of variants detected previously by the validated laboratory were also identified in our analysis. Then, comparing the hybridization capture-based library preparation methodology with the amplicon-based strategy, we found ~94% concordance between identified actionable variants across the 15 shared genes, analyzed by the TumorSec^TM bioinformatics pipeline, developed by the Cancer Genomics Laboratory. Our results demonstrate that it is entirely viable to implement an NGS-based analysis of actionable variant identification and prioritization in cancer samples in our laboratory, being part of the Chilean public health system and paving the way to improve the access to such analyses. Considering the economic realities of most Latin American countries, using a small NGS panel, such as TumorSec^TM, focused on relevant variants of the Chilean and Latin American population is a cost-effective approach to extensive global NGS panels. Furthermore, the incorporation of automated bioinformatics analysis in this streamlined assay holds the potential of facilitating the implementation of precision medicine in this geographic region, which aims to greatly support personalized treatment of cancer patients in Chile. Full article

Carbon dioxide (CO₂) emissions due to human activities are responsible for approximately 80% of the drivers of global warming, resulting in a 1.1 °C increase above pre-industrial temperatures. This study quantified the CO₂ assimilation and productivity of the brown macroalgae Lessonia spicata in the central Pacific coast of Chile, across seasonal and daily cycles, under different environmental stressors, such as temperature and solar irradiance. Measurements were performed using an infra-red gas analysis (IRGA) instrument which had a chamber allowing for precise quantification of CO₂ concentrations; additional photophysiological and biochemical responses were also measured. CO₂ assimilation, along with the productivity and biosynthesis of proteins and lipids, increased during the spring, coinciding with moderate temperatures (~14 °C) and high photosynthetically active radiation (PAR). Furthermore, the increased production of photoprotective and antioxidant compounds, including phenolic compounds, and carotenoids, along with the enhancement of non-photochemical quenching (NPQ), contribute to the effective photoacclimation strategies of L. spicata. Principal component analysis (PCA) revealed seasonal associations between productivity, reactive oxygen species (ROSs), and biochemical indicators, particularly during the spring and summer. These associations, further supported by Pearson correlation analyses, suggest a high but seasonally constrained photoacclimation capacity. In contrast, the reduced productivity and photoprotection observed in the summer suggest increased physiological vulnerability to heat and light stress. Overall, our findings position L. spicata as a promising nature-based solution for climate change mitigation. Full article

This study aims to investigate the processes associated with mass movements and their relationship with the behavior of the Amazon River delta-estuary (ADE) wetlands. The methodological approach involves using water spectral indices and ground-penetrating radar (GPR) to diagnose areas of soil water saturation and characterize regions affected by mass movements in Amazonian cities. It also involves identifying areas of critical saturation content and consequent mass movements. Analysis of risk and land use data revealed that the affected areas coincide with zones of high susceptibility to mass movements induced by water. The results showed the following: the accumulated annual precipitation ranged from 70.07 ± 55.35 mm·month⁻¹ to 413.34 ± 127.51 mm·month⁻¹; the response similarity across different sensors obtained an accuracy greater than 90% for NDWI, MNDWI, and AWEI for the same targets; and a landfill layer with a thickness variation between 1 and 2 m defined the mass movement concentration in Abaetetuba city. The interaction between infiltration, water saturation, and human-induced land alteration suggests that these areas act as wetlands with unstable dynamics. The analysis methodology developed for this study aimed to address this scenario by systematically mapping areas with mass movement potential and high-water saturation. Due to the absence of geological and geotechnical data, remote sensing was employed as an alternative, and in situ ground-penetrating radar (GPR) evaluation was suggested as a means of investigating the causes of a previously observed movement. Full article

Cannabis sativa L. is a versatile plant with significant medicinal, industrial, and recreational applications. Its therapeutic potential is attributed to cannabinoids like THC and CBD, whose production is influenced by environmental factors, such as radiation, temperature, and humidity. Radiation, for instance, is essential for photosynthetic processes, acting as both a primary energy source and a regulator of plant growth and development. This review covers key factors affecting C. sativa cultivation, including photoperiod, light spectrum, cultivation methods, environmental controls, and plant growth regulators. It highlights how these elements influence flowering, biomass, and cannabinoid production across different growing systems, offering insights for optimizing both medicinal and industrial cannabis cultivation. Studies indicate that photoperiod sensitivity varies among cultivars, with some achieving optimal flowering and cannabinoid production under extended light periods rather than the traditional 12/12 h cycle. Light spectrum adjustments, especially red, far-red, and blue wavelengths, significantly impact photosynthesis, plant morphology, and secondary metabolite accumulation. Advances in LED technology allow precise spectral control, enhancing energy efficiency and cannabinoid profiles compared to conventional lighting. The photoperiod plays a vital role in the cultivation of C. sativa spp., directly impacting the plant’s developmental cycle, biomass production, and the concentration of cannabinoids and terpenes. The response to photoperiod varies among different cannabis cultivars, as demonstrated in studies comparing cultivars of diverse genetic origins. On the other hand, indoor or in vitro cultivation may serve as an excellent alternative for plant breeding programs in C. sativa, given the substantial inter-cultivar variability that hinders the fixation of desirable traits. Full article