Search Results (891)

Pantoea stewartii subsp. stewartii (Pss) is a Gram-negative bacterium first documented in North America, and is the causal agent of Stewart’s disease in maize (Zea mays), especially in sweet corn. First identified in North America, it is primarily spread by insect vectors like the corn flea beetle (Chaetocnema Pulicaria) in the United States. However, Pss has since spread globally—reaching parts of Africa, Asia, the Americas, and Europe—mainly through the international seed trade. Although this trade is limited, it has still facilitated the pathogen’s global movement, as evidenced by numerous phytosanitary interceptions. Recent studies in Italy, as indicated in the EFSA journal, reported that potential alternative vectors were identified, including Phyllotreta spp. and the invasive Asian brown marmorated stink bug (Halyomorpha halys); the latter tested positive in PCR screenings, raising concerns due to its broad host range and global distribution. This information has prompted studies to verify the ability of Halyomorpha halys to vector Pss to assess the risk and prevent the further spread of Pss in Europe. In this study, we explored the potential transmission of Pss by the brown marmorated stink bugs in maize plants, following its feeding on Pss-inoculated maize, as well as the presence of Pss within the insect’s body. Full article

UV-C irradiation is an innovative postharvest technique for increasing the safety of fruits and vegetables. This study investigated the effect of UV-C rays (UV-C1 = 0.26 KJ/m²; UV-C2 = 0.40 KJ/m²; UV-C3 = 0.67 KJ/m²; and UV-C4 = 1.34 KJ/m²) on the preservation of the antioxidants, hardness, and color of fresh green asparagus during storage. UV-C1 and UV-C2 significantly maintained higher total phenolic content (10.6%), total flavonoid content (36%), rutin (14.3%), quercetin (27.03%), kaempferol-3-O-rutinoside (21.25%), and antioxidant activity (DPPH 7.5%). Over three weeks of storage, quercetin, ferulic acid, and kaempferol 3-O-rutinoside increased, while rutin and caffeic acid decreased. Storage caused a significant change in the color and hardness of the control sample, but UV-C4 counteracted hardening for up to three weeks, and UV-C3 was the best dose for stabilizing color during storage. This study indicates that the choice of UV-C dose can be modulated based on the characteristics that are intended to be preserved in green asparagus, maintaining a balance between nutraceutical and hedonic characteristics. To maintain the maximum level of nutraceutical compounds over time, UV-C2 can be adopted, while to preserve texture and color, UV-C3 and UV-C4 are a better choice. Full article

Infrared ship detection plays a vital role in maritime surveillance systems. As a critical remote sensing application, it enables maritime surveillance across diverse geographic scales and operational conditions while offering robust all-weather operation and resilience to environmental interference. However, infrared imagery in complex maritime environments presents significant challenges, including low contrast, background clutter, and difficulties in detecting small-scale or distant targets. To address these issues, we propose an Infrared Ship Detection Network (IRSD-Net), a lightweight and efficient detection network built upon the YOLOv11n framework and specially designed for infrared maritime imagery. IRSD-Net incorporates a Hierarchical Multi-Kernel Convolution Network (HMKCNet), which employs parallel multi-kernel convolutions and channel division to enhance multi-scale feature extraction while reducing redundancy and memory usage. To further improve cross-scale fusion, we design the Dynamic Cross-Scale Feature Pyramid Network (DCSFPN), a bidirectional architecture that combines up- and downsampling to integrate low-level detail with high-level semantics. Additionally, we introduce Wise-PIoU, a novel loss function that improves bounding box regression by enforcing geometric alignment and adaptively weighting gradients based on alignment quality. Experimental results demonstrate that IRSD-Net achieves 92.5% ${\mathrm{mAP}}_{50}$ on the ISDD dataset, outperforming YOLOv6n and YOLOv11n by 3.2% and 1.7%, respectively. With a throughput of 714.3 FPS, IRSD-Net delivers high-accuracy, real-time performance suitable for practical maritime monitoring systems. Full article

This study presents a detailed analysis of the combustion dynamics of stoichiometric H₂–air and CH₄–air mixtures in a 20 L closed vessel over an initial temperature range of 298–423 K. We integrate experimental pressure–time P(t) measurements with numerical analysis to extract laminar burning velocity (LBV) and deflagration index (K_G) values, and we assess three independent kinetic mechanisms (KiBo_MU, University of San Diego, Lund University) via simulations. For H₂–air, LBV increases from 0.50 m/s at 298 K to 0.94 m/s at 423 K (temperature exponent α ≈ 1.79), while for CH₄–air, LBV rises from 0.36 m/s to 0.96 m/s (α ≈ 2.82). In contrast, the deflagration index K_G decreases by ca. 20% for H₂–air and ca. 30% for CH₄–air over the same temperature span. The maximum explosion pressure (P_max) and peak pressure rise rate ((dP/dt)_max) also exhibit systematic increases with temperature. A comparison with model predictions shows agreement within experiments, providing data for safety modeling and kinetic mechanism validation in H₂- and CH₄-based energy systems. Full article

Background/Objectives: Spacer dislocation is among the most frequent mechanical complications after revision total hip arthroplasty for periprosthetic hip infection. Spacer dislocations may be managed conservatively, but there are no guidelines on the rehabilitation of these patients, and the restriction of weight bearing is still under debate. Methods: We first report the case of a patient with hip spacer cranial dislocation, judged unfit to be surgically treated once more for a medium period, who started a rehabilitation program with partial weight bearing. Results: After two weeks of inpatient rehabilitation, the patient started to maintain the standing position with partial weight bearing on the affected side. Following hospital discharge we continued rehabilitation in the outpatient clinic. Despite the finding of the denervation of the ipsilateral quadriceps, three months after admission, she was able to walk for short distances using a walker, initially with the help of a therapist and then with supervision. About one year later, she was able to undergo the reimplantation of the definitive prosthesis. Conclusions: Despite the spacer dislocation, walking short distances is a feasible goal, even with assistance, wearing a brace and using a walker. Future research is needed to confirm and expand upon this observation and to understand the mechanisms underlying the development of neurological complications to implement effective prevention strategies. Full article

Introduction: Obstructive sleep apnea syndrome (OSAS) is caused by anatomical and non-anatomical factors which lead to upper airway (UA) obstruction during sleep. Intrinsic UA collapse is the most frequent determinant of OSA. In the era of personalized medicine, adopting a tailored diagnostic approach is essential to rule out secondary causes of UA collapse, particularly those stemming from extrinsic anatomical factors. Although being rarely considered in the differential diagnosis, space-occupying lesions of deep cervical spaces such as the parapharyngeal space (PPS) may be responsible for airway obstruction and lead to OSAS. Objective: This study aimed to present an atypical case of OSAS caused by extrinsic PPS compression, outlining the relevance of modern personalized medicine in the diagnostic and therapeutic protocols, and to enhance understanding through a comprehensive literature review. Methods: A 60-year-old female presented with sleep-disordered complaints and was diagnosed with severe OSAS after polysomnography. At physical examination, a swelling of the right posterior oropharyngeal mucosa was noticed. Imaging confirmed the suspicion of a PPS tumor, and transcervical resection was planned. Case presentation was adherent to the CARE checklist. A comprehensive literature review was conducted using the most reliable scientific databases. Results: Surgery was uneventful, and the patient made a full recovery. The histopathology report was consistent with the diagnosis of pleomorphic adenoma. Postoperative outcomes showed marked improvement in polysomnographic parameters and symptom burden. Conclusions: Parapharyngeal space tumors are a rare, often overlooked cause of OSA. This case highlights the role of a personalized head and neck assessment in OSA patients, particularly in identifying structural causes and offering definitive surgical management when indicated. Full article

Background/Objectives: Asprosin is the endogenous ligand of the olfactory Olfr734 receptor linked to MASLD and glucose metabolism. Despite the involvement of asprosin in these processes, little has been published on the specific role of Olfr734 in liver function. The aim of this work is therefore to study the specific role of the olfactory Olfr734 receptor in MASLD and glucose metabolism. Methods: To achieve this objective, we performed a genetic inhibition specifically to inhibit Olfr734 in the livers of male mice. We then studied the progression of MASLD in DIO mice. In addition, we studied the glucose metabolism in hypoglycemia states and postprandial glucose production in standard diet-fed mice. Finally, analyses of liver biopsies from patients with obesity and with or without T2DM were conducted. Results: We found that hepatic Olfr734 levels vary according to changes in nutritional status and its knockdown effect in the liver is to increase the hepatic lipid content in DIO mice. Our results also showed that OLFR734 expression is involved in the adaptive response in terms of glucose production to nutrient availability. Finally, the hepatic human Olfr734 ortholog named OR4M1 has been observed to be at significantly higher levels in male patients with T2DM. Conclusions: This study increases understanding of the mechanisms by which the modulation of Olfr734 expression affects liver function. Full article

The nutrient requirements of coffee plants vary according to their phenological stages, with each nutrient playing specific roles in different structures and developmental phases. This study evaluated dry matter accumulation and the concentrations of N, P, K, Ca, Mg, S, Fe, Mn, Cu, Zn, and B in the leaves, branches, and reproductive organs of five Coffea canephora genotypes during three phenophases: flowering, fruit development, and fruit ripening. This work aimed to evaluate the distribution of nutrients in three phenophases in Coffeea canephora genotypes. Significant differences were observed among genotypes and phenophases. During flowering, leaves accumulated the highest amount of dry matter, but this pattern reversed in later stages, with greater accumulation in the fruits, especially during fruit ripening. The Verdim TA genotype showed the lowest dry matter accumulation in the branches across all phenophases. Genotypes A1 and Clementino presented the highest mean concentrations of P, Ca, Mg, Fe, Cu, and Zn in the leaves during the fruit development phase, while Verdim TA showed the lowest concentrations of P, K, Ca, Mn, Zn, and B. Future studies may include additional phenological stages and quantify nutrient remobilization efficiency in each genotype, contributing to improved management recommendation. Full article

This study investigates the potential of Eucalyptus benthamii wood from planted forests in southern Brazil for the production of cross-laminated timber (CLT) panels. The performance of E. benthamii CLT panels is compared to that of Pinus spp. panels and European commercial panels (KLH^®), using the finite element method applied to a two-story building model. Class 2 of the Brazilian standard ABNT NBR 7190-2 was adopted as the reference for the physical and mechanical properties of Pinus spp., while the European commercial specifications from KLH^® were used to represent European reference panels. The results indicate that E. benthamii wood exhibits superior mechanical properties, enabling reductions of 12.5% to 27.3% in panel thickness and a 20.7% decrease in wood volume when compared to Pinus spp., without compromising structural safety. Relative to the KLH^® and ETA 06/0138 standards, E. benthamii wood demonstrates higher stiffness (modulus of elasticity of 15,325 MPa vs. 12,000 MPa) and greater flexural strength (109.11 MPa vs. 24 MPa), allowing for the use of thinner panels. Stress and displacement analyses confirm that E. benthamii CLT slabs can withstand critical loads (wind and vertical) within normative limits, with maximum displacements of 18.5 mm. The reduction in material volume (22.8 m³ versus 28.7 m³ for Pinus spp.) suggests potential benefits in terms of environmental impact and logistical efficiency. It can be concluded that E. benthamii represents a sustainable and efficient alternative for CLT panels, combining high structural performance with resource optimization and contributing to the decarbonization of the construction industry. Full article

Grapevine breeding programs face difficulties in preserving germplasm, especially from species and interspecific hybrids, since most collections are maintained in the field and exposed to biotic and abiotic stress, which can lead to material loss. The Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF) Grapevine Breeding Program faces similar challenges, limiting studies on hybrids resistant to the nematode Pratylenchus brachyurus and downy mildew (Plasmopara viticola), which are valuable for genetic improvement. This study aimed to implement in vitro conservation under minimal growth conditions for interspecific hybrids of Vitis spp. from the UENF program. The protocol followed a completely randomized design in a 2 × 2 × 3 factorial scheme: two hybrids (CH1.2 and CH1.3), two temperatures (18 ± 1 °C and 27 ± 2 °C), and three sucrose concentrations (10, 20, and 30 g L⁻¹), over 180 days of in vitro culture. The results showed that conservation of the UENF hybrids is feasible using nodal segments as explants, at 18 ± 2 °C and 10 g L⁻¹ of sucrose, for up to four months. This protocol may also be applied to other Vitis spp., contributing to the preservation and continued study of valuable germplasm. Full article

Background: The ROSA Knee System (Zimmer Biomet, Warsaw, IN, USA) is a robotic system aiming to increase bone resections and component alignment accuracy during TKA. While much is known about its performance in the coronal plane, its accuracy in the sagittal plane remains debated. The present investigation evaluated the system’s accuracy in achieving planned mechanical axis alignment and specific knee angles in both planes. Methods: A retrospective analysis was performed on 55 consecutive patients who underwent robotic-assisted TKA using the ROSA Knee System. Data on the medial proximal tibial angle (MPTA), lateral distal femoral angle (LDFA), hip–knee–ankle angle (HKA), tibial slope (TS), and distal femoral flexion (DFF) were collected pre- and post-operatively using the ROSA software. Planned and achieved angles were compared, with deviations greater than 2° and 3° defined as outliers. Results: The mean differences between planned and achieved angles for LDFA and MPTA were 0.5° ± 1.00° and 0.3° ± 1.3°, respectively, with less than 10% outliers. The hip–knee angle recorded only a minimal deviation from planned values. In contrast, the TS angle showed a statistically significant difference between planned and achieved values, while no significant difference was found for the DFF angle. The surgeon’s experience did not impact alignment accuracy. Conclusions: The ROSA Knee System demonstrates high accuracy in achieving planned alignment in the coronal plane during robotic-assisted TKA, with minimal outliers and reliable predictions for both femoral and tibial angles. However, the ROSA Knee System showed less accuracy in the sagittal plane, particularly for the tibial slope, which did not adversely affect the implant’s stability. Full article

Accurate pig counting is crucial for precision livestock farming, enabling optimized feeding management and health monitoring. Detection-based counting methods face significant challenges due to mutual occlusion, varying illumination conditions, diverse pen configurations, and substantial variations in pig densities. Previous approaches often struggle with complex agricultural environments where lighting conditions, pig postures, and crowding levels create challenging detection scenarios. To address these limitations, we propose EAPC-YOLO (enhanced adaptive pig counting YOLO), a robust architecture integrating density-aware processing with advanced detection optimizations. The method consists of (1) an enhanced YOLOv8 network incorporating multiple architectural improvements for better feature extraction and object localization. These improvements include DCNv4 deformable convolutions for irregular pig postures, BiFPN bidirectional feature fusion for multi-scale information integration, EfficientViT linear attention for computational efficiency, and PIoU v2 loss for improved overlap handling. (2) A density-aware post-processing module with intelligent NMS strategies that adapt to different crowding scenarios. Experimental results on a comprehensive dataset spanning diverse agricultural scenarios (nighttime, controlled indoor, and natural daylight environments with density variations from 4 to 30 pigs) demonstrate our method achieves 94.2% mAP@0.5 for detection performance and 96.8% counting accuracy, representing 12.3% and 15.7% improvements compared to the strongest baseline, YOLOv11n. This work enables robust, accurate pig counting across challenging agricultural environments, supporting precision livestock management. Full article

Human papillomavirus (HPV) is a recognized oncogenic agent in several epithelial malignancies, though its role in esophageal squamous lesions remains unclear. Esophageal squamous papilloma and papillomatosis are rare, often benign lesions, but increasing evidence suggests possible associations with high-risk HPV genotypes and a non-negligible risk of dysplasia and malignant transformation. This narrative review summarizes current evidence on epidemiology, clinical features, histopathology, and diagnostic approaches, emphasizing advanced endoscopic imaging techniques that improve lesion detection and characterization. Management relies primarily on complete endoscopic resection with histological and virological evaluation. While small, non-dysplastic solitary lesions may not require routine surveillance, multifocal or high-risk HPV-positive cases warrant closer follow-up. Standardized HPV testing and long-term prospective studies are needed to better define the oncogenic potential and inform surveillance and treatment strategies. Full article

In Italy, the law on road homicide (Law no. 41/2016) introduced specific provisions for drivers who cause severe injuries or death to a person due to the violation of the Highway Code. The use of alcohol or drugs while driving constitutes an aggravating circumstance of the offence and provides for a tightening of penalties. Our study aims to report on the analysis performed on blood samples collected between January 2018 and December 2024 from drivers convicted of road homicide and who tested positive for alcohol and/or drugs. The majority of the involved subjects were males belonging to the 18–30 and 41–50 age groups. Alcohol, cocaine and cannabinoids were the most detected substances and the most frequent polydrug combination was alcohol and cocaine. We also investigated other influencing factors in road traffic accidents as the day of the week and the time of the day in which fatal road traffic accident occurred, and the time elapsed between the road accident and the collection of biological samples. Our data, in line with the international scenario, strongly support that, in addition to the tightening of penalties, raising awareness plays a key role in preventing alcohol- and drug-related traffic accidents by increasing risk perception and encouraging safer driving behaviors. Full article

Background: Advanced and recurrent vulvar squamous cell carcinoma (VSCC) presents a major therapeutic challenge with limited treatment options and poor outcomes. Immune checkpoint inhibitors (ICIs) have shown efficacy in other HPV-associated malignancies, but their role in VSCC remains poorly defined due to the rarity of the disease and limited clinical trial data. Methods: We conducted a systematic review and meta-analysis following PRISMA guidelines and registered in PROSPERO (CRD420251067565). A comprehensive literature search identified prospective clinical trials evaluating ICIs in patients with advanced, unresectable, recurrent, or metastatic VSCC. The primary outcomes included objective response rate (ORR), progression-free survival (PFS), overall survival (OS), and safety. Risk of bias was assessed using the MINORS tool. Meta-analyses were performed using random-effects models, with subgroup analyses based on PD-L1 status and treatment regimens (monotherapy vs. combination therapy). Results: Six non-randomized single-arm trials involving 181 patients were included. The pooled ORR was 21%, with higher response rates observed in combination therapy (46%) compared to monotherapy (11%), though not statistically significant. Median PFS and OS were 2.2 months and 6.4 months, respectively. ORRs were similar between PD-L1-positive and PD-L1-negative subgroups. A safety analysis showed treatment-related adverse events (AEs) in 73% of patients and grade ≥ 3 AEs in 23%. The incidence of treatment-related death was 3%. Conclusions: ICIs demonstrate modest but durable efficacy and an acceptable safety profile in advanced VSCC. The current evidence supports their use in selected patients. However, response variability and the lack of reliable predictive biomarkers, such as PD-L1 or HPV status, underscore the need for biomarker-driven clinical trials and improved patient selection strategies. Full article