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16 pages, 1141 KiB  
Article
Coordinated Roles of Osmotic Adjustment, Antioxidant Defense, and Ion Homeostasis in the Salt Tolerance of Mulberry (Morus alba L. ‘Tailai Sang’) Seedlings
by Nan Xu, Tiane Wang, Yuan Wang, Juexian Dong and Yu Shaopeng
Forests 2025, 16(8), 1258; https://doi.org/10.3390/f16081258 (registering DOI) - 1 Aug 2025
Abstract
Soil salinization severely limits plant growth and productivity. Mulberry (Morus alba L.), an economically and ecologically important tree, is widely cultivated, yet its salt-tolerance mechanisms at the seedling stage remain insufficiently understood. This study investigated the physiological and biochemical responses of two-year-old [...] Read more.
Soil salinization severely limits plant growth and productivity. Mulberry (Morus alba L.), an economically and ecologically important tree, is widely cultivated, yet its salt-tolerance mechanisms at the seedling stage remain insufficiently understood. This study investigated the physiological and biochemical responses of two-year-old mulberry (‘Tailai Sang’) seedlings subjected to six NaCl treatments (0, 50, 100, 150, 200, and 300 mmol L−1) for 28 days. Results showed that growth parameters and photosynthetic gas exchange exhibited dose-dependent declines. The reduction in net photosynthetic rate (Pn) was attributed to both stomatal limitations (decreased stomatal conductance) and non-stomatal limitations, as evidenced by a significant decrease in the maximum quantum efficiency of photosystem II (Fv/Fm) under high salinity. To cope with osmotic stress, seedlings accumulated compatible solutes, including soluble sugars, proteins, and proline. Critically, mulberry seedlings demonstrated effective ion homeostasis by sequestering Na+ in the roots to maintain a high K+/Na+ ratio in leaves, a mechanism that was compromised above 150 mmol L−1. Concurrently, indicators of oxidative stress—malondialdehyde (MDA) and H2O2—rose significantly with salinity, inducing the activities of antioxidant enzymes (SOD, CAT, APX, and GR), which peaked at 150 mmol L−1 before declining under extreme stress. A biomass-based LC50 of 179 mmol L−1 NaCl was determined. These findings elucidate that mulberry salt tolerance is a coordinated process involving three key mechanisms: osmotic adjustment, selective ion distribution, and a robust antioxidant defense system. This study establishes an indicative tolerance threshold under controlled conditions and provides a physiological basis for further field-based evaluations of ‘Tailai Sang’ mulberry for cultivation on saline soils. Full article
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13 pages, 553 KiB  
Article
Biorefinery-Based Energy Recovery from Algae: Comparative Evaluation of Liquid and Gaseous Biofuels
by Panagiotis Fotios Chatzimaliakas, Dimitrios Malamis, Sofia Mai and Elli Maria Barampouti
Fermentation 2025, 11(8), 448; https://doi.org/10.3390/fermentation11080448 (registering DOI) - 1 Aug 2025
Abstract
In recent years, biofuels and bioenergy derived from algae have gained increasing attention, fueled by the growing demand for renewable energy sources and the urgent need to lower CO2 emissions. This research examines the generation of bioethanol and biomethane using freshly harvested [...] Read more.
In recent years, biofuels and bioenergy derived from algae have gained increasing attention, fueled by the growing demand for renewable energy sources and the urgent need to lower CO2 emissions. This research examines the generation of bioethanol and biomethane using freshly harvested and sedimented algal biomass. Employing a factorial experimental design, various trials were conducted, with ethanol yield as the primary optimization target. The findings indicated that the sodium hydroxide concentration during pretreatment and the amylase dosage in enzymatic hydrolysis were key parameters influencing the ethanol production efficiency. Under optimized conditions—using 0.3 M NaOH, 25 μL/g starch, and 250 μL/g cellulose—fermentation yielded ethanol concentrations as high as 2.75 ± 0.18 g/L (45.13 ± 2.90%), underscoring the significance of both enzyme loading and alkali treatment. Biomethane potential tests on the residues of fermentation revealed reduced methane yields in comparison with the raw algal feedstock, with a peak value of 198.50 ± 25.57 mL/g volatile solids. The integrated process resulted in a total energy recovery of up to 809.58 kWh per tonne of algal biomass, with biomethane accounting for 87.16% of the total energy output. However, the energy recovered from unprocessed biomass alone was nearly double, indicating a trade-off between sequential valorization steps. A comparison between fresh and dried feedstocks also demonstrated marked differences, largely due to variations in moisture content and biomass composition. Overall, this study highlights the promise of integrated algal biomass utilization as a viable and energy-efficient route for sustainable biofuel production. Full article
(This article belongs to the Special Issue Algae Biotechnology for Biofuel Production and Bioremediation)
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13 pages, 1267 KiB  
Article
Toward Green Substitutes: Electrochemical Properties of Conductive Inks Containing Biochar
by Gordana Stevanović, Jovan Parlić, Marija Ajduković, Nataša Jović-Jovičić, Vojkan Radonjić and Zorica Mojović
Sustain. Chem. 2025, 6(3), 21; https://doi.org/10.3390/suschem6030021 - 31 Jul 2025
Abstract
Dry Turkish oak (Quercus cerris) sawdust, untreated and treated with three activators, (H3PO4, NaOH and H2O2) was pyrolyzed under limited-oxygen conditions to obtain biochar samples. The electrochemical properties of these samples were tested [...] Read more.
Dry Turkish oak (Quercus cerris) sawdust, untreated and treated with three activators, (H3PO4, NaOH and H2O2) was pyrolyzed under limited-oxygen conditions to obtain biochar samples. The electrochemical properties of these samples were tested and compared to the properties of several commercial carbon blacks. The electrochemical characterization was performed via cyclic voltammetry, analyzing the response toward two commonly used redox probes, [Fe(CN)6]3−/−4− and [Ru(NH3)6]2+/3+. The influence of the scan rate on this response was investigated, and the resulting data were used to obtain the values of the heterogenous charge transfer constant, k0. Higher k0 values were observed for carbon blacks than for investigated biochar samples. The detection of 4-nitrophenol and heavy metal ions was used to assess the applicability of biochars for electroanalytical purposes. The response of untreated biochar was comparable with the response of Vulcan carbon black, which showed the best response of all analyzed carbon blacks. Full article
16 pages, 2729 KiB  
Article
Effect of Enterobacter bugandensis R-18 on Maize Growth Promotion Under Salt Stress
by Xingguo Tian, Qianru Liu, Jingjing Song, Xiu Zhang, Guoping Yang, Min Li, Huan Qu, Ahejiang Tastanbek and Yarong Tan
Microorganisms 2025, 13(8), 1796; https://doi.org/10.3390/microorganisms13081796 - 31 Jul 2025
Abstract
Soil salinization poses a significant constraint to agricultural productivity. However, certain plant growth-promoting bacteria (PGPB) can mitigate salinity stress and enhance crop performance. In this study, a bacterial isolate, R-18, isolated from saline-alkali soil in Ningxia, China, was identified as Enterobacter bugandensis based [...] Read more.
Soil salinization poses a significant constraint to agricultural productivity. However, certain plant growth-promoting bacteria (PGPB) can mitigate salinity stress and enhance crop performance. In this study, a bacterial isolate, R-18, isolated from saline-alkali soil in Ningxia, China, was identified as Enterobacter bugandensis based on 16S rRNA gene sequencing. The isolate was characterized for its morphological, biochemical, and plant growth-promoting traits and was evaluated for its potential to alleviate NaCl-induced stress in maize (Zea mays L.) under hydroponic conditions. Isolate R-18 exhibited halotolerance, surviving at NaCl concentrations ranging from 2.0% to 10.0%, and alkaliphilic adaptation, growing at pH 8.0–11.0. Biochemical assays confirmed it as a Gram-negative bacterium, displaying positive reactions in the Voges–Proskauer (V–P) tests, catalase activity, citrate utilization, fluorescent pigment production, starch hydrolysis, gelatin liquefaction, and ammonia production, while testing negative for the methyl red and cellulose hydrolysis. Notably, isolate R-18 demonstrated multiple plant growth-promoting attributes, including nitrogen fixation, phosphate and potassium solubilization, ACC deaminase activity, and indole-3-acetic acid (IAA) biosynthesis. Under 100 mM NaCl stress, inoculation with isolate R-18 significantly enhanced maize growth, increasing plant height, stem dry weight, root fresh weight, and root dry weight by 20.64%, 47.06%, 34.52%, and 31.25%, respectively. Furthermore, isolate R-18 improved ion homeostasis by elevating the K+/Na+ ratio in maize tissues. Physiological analyses revealed increased chlorophyll and proline content, alongside reduced malondialdehyde (MDA) levels, indicating mitigated oxidative damage. Antioxidant enzyme activity was modulated, with decreased superoxide dismutase (SOD) and peroxidase (POD) activities but increased catalase (CAT) activity. These findings demonstrated that Enterobacter bugandensis R-18 effectively alleviated NaCl-induced growth inhibition in maize by enhancing osmotic adjustment, reducing oxidative stress, and improving ion balance. Full article
(This article belongs to the Section Plant Microbe Interactions)
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20 pages, 4874 KiB  
Article
Influence of Vegetation Cover and Soil Properties on Water Infiltration: A Study in High-Andean Ecosystems of Peru
by Azucena Chávez-Collantes, Danny Jarlis Vásquez Lozano, Leslie Diana Velarde-Apaza, Juan-Pablo Cuevas, Richard Solórzano and Ricardo Flores-Marquez
Water 2025, 17(15), 2280; https://doi.org/10.3390/w17152280 - 31 Jul 2025
Abstract
Water infiltration into soil is a key process in regulating the hydrological cycle and sustaining ecosystem services in high-Andean environments. However, limited information is available regarding its dynamics in these ecosystems. This study evaluated the influence of three types of vegetation cover and [...] Read more.
Water infiltration into soil is a key process in regulating the hydrological cycle and sustaining ecosystem services in high-Andean environments. However, limited information is available regarding its dynamics in these ecosystems. This study evaluated the influence of three types of vegetation cover and soil properties on water infiltration in a high-Andean environment. A double-ring infiltrometer, the Water Drop Penetration Time (WDPT, s) method, and laboratory physicochemical characterization were employed. Soils under forest cover exhibited significantly higher quasi-steady infiltration rates (is, 0.248 ± 0.028 cm·min−1) compared to grazing areas (0.051 ± 0.016 cm·min−1) and agricultural lands (0.032 ± 0.013 cm·min−1). Soil organic matter content was positively correlated with is. The modified Kostiakov infiltration model provided the best overall fit, while the Horton model better described infiltration rates approaching is. Sand and clay fractions, along with K+, Ca2+, and Mg2+, were particularly significant during the soil’s wet stages. In drier stages, increased Na+ concentrations and decreased silt content were associated with higher water repellency. Based on WDPT, agricultural soils exhibited persistent hydrophilic behavior even after drying (median [IQR] from 0.61 [0.38] s to 1.24 [0.46] s), whereas forest (from 2.84 [3.73] s to 3.53 [24.17] s) and grazing soils (from 4.37 [1.95] s to 19.83 [109.33] s) transitioned to weakly or moderately hydrophobic patterns. These findings demonstrate that native Andean forest soils exhibit a higher infiltration capacity than soils under anthropogenic management (agriculture and grazing), highlighting the need to conserve and restore native vegetation cover to strengthen water resilience and mitigate the impacts of land-use change. Full article
(This article belongs to the Special Issue Soil–Water Interaction and Management)
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26 pages, 6611 KiB  
Article
The Geochronology, Geochemical Characteristics, and Tectonic Settings of the Granites, Yexilinhundi, Southern Great Xing’an Range
by Haixin Yue, Henan Yu, Zhenjun Sun, Yanping He, Mengfan Guan, Yingbo Yu and Xi Chen
Minerals 2025, 15(8), 813; https://doi.org/10.3390/min15080813 (registering DOI) - 31 Jul 2025
Viewed by 28
Abstract
The southern Great Xing’an Range is located in the overlap zone of the Paleo-Asian Ocean metallogenic domain and the Circum-Pacific metallogenic domain. It hosts numerous Sn-polymetallic deposits, such as Weilasituo, Bianjiadayuan, Huanggang, and Dajing, and witnessed multiple episodes of magmatism during the Late [...] Read more.
The southern Great Xing’an Range is located in the overlap zone of the Paleo-Asian Ocean metallogenic domain and the Circum-Pacific metallogenic domain. It hosts numerous Sn-polymetallic deposits, such as Weilasituo, Bianjiadayuan, Huanggang, and Dajing, and witnessed multiple episodes of magmatism during the Late Mesozoic. The study area is situated within the Huanggangliang-Ganzhuermiao metallogenic belt in the southern Great Xing’an Range. The region has witnessed extensive magmatism, with Mesozoic magmatic activities being particularly closely linked to regional mineralization. We present petrographic, zircon U-Pb chronological, lithogeochemical, and Lu-Hf isotopic analyses of the Yexilinhundi granites. The results indicate that the granite porphyry and granodiorite were emplaced during the Late Jurassic. Both rocks exhibit high SiO2, K2O + Na2O, differentiation index (DI), and 10,000 Ga/Al ratios, coupled with low MgO contents. They show distinct fractionation between light and heavy rare earth elements (LREEs and HREEs), exhibit Eu anomalies, and have low whole-rock zircon saturation temperatures (Tzr), collectively demonstrating characteristics of highly fractionated I-type granites. The εHf(t) values of the granites range from 0.600 to 9.14, with young two-stage model ages (TDM2 = 616.0~1158 Ma), indicating that the magmatic source originated from partial melting of Mesoproterozoic-Neoproterozoic juvenile crust. This study proposes that the granites formed in a post-collisional/post-orogenic extensional setting associated with the subduction of the Mongol-Okhotsk Ocean, providing a scientific basis for understanding the relationship between the formation of Sn-polymetallic deposits and granitic magmatic evolution in the study area. Full article
(This article belongs to the Section Mineral Geochemistry and Geochronology)
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14 pages, 2351 KiB  
Article
Facile SEI Improvement in the Artificial Graphite/LFP Li-Ion System: Via NaPF6 and KPF6 Electrolyte Additives
by Sepehr Rahbariasl and Yverick Rangom
Energies 2025, 18(15), 4058; https://doi.org/10.3390/en18154058 (registering DOI) - 31 Jul 2025
Viewed by 75
Abstract
In this work, graphite anodes and lithium iron phosphate (LFP) cathodes are used to examine the effects of sodium hexafluorophosphate (NaPF6) and potassium hexafluorophosphate (KPF6) electrolyte additives on the formation of the solid electrolyte interphase and the performance of [...] Read more.
In this work, graphite anodes and lithium iron phosphate (LFP) cathodes are used to examine the effects of sodium hexafluorophosphate (NaPF6) and potassium hexafluorophosphate (KPF6) electrolyte additives on the formation of the solid electrolyte interphase and the performance of lithium-ion batteries in both half-cell and full-cell designs. The objective is to assess whether these additives may increase cycle performance, decrease irreversible capacity loss, and improve interfacial stability. Compared to the control electrolyte (1.22 M Lithium hexafluorophosphate (LiPF6)), cells with NaPF6 and KPF6 additives produced less SEI products, which decreased irreversible capacity loss and enhanced initial coulombic efficiency. Following the formation of the solid electrolyte interphase, the specific capacity of the control cell was 607 mA·h/g, with 177 mA·h/g irreversible capacity loss. In contrast, irreversible capacity loss was reduced by 38.98% and 37.85% in cells containing KPF6 and NaPF6 additives, respectively. In full cell cycling, a considerable improvement in capacity retention was achieved by adding NaPF6 and KPF6. The electrolyte, including NaPF6, maintained 67.39% greater capacity than the LiPF6 baseline after 20 cycles, whereas the electrolyte with KPF6 demonstrated a 30.43% improvement, indicating the positive impacts of these additions. X-ray photoelectron spectroscopy verified that sodium (Na+) and potassium (K+) ions were present in the SEI of samples containing NaPF6 and KPF6. While K+ did not intercalate in LFP, cyclic voltammetry confirmed that Na+ intercalated into LFP with negligible impact on the energy storage of full cells. These findings demonstrate that NaPF6 and KPF6 are suitable additions for enhancing lithium-ion battery performance in the popular artificial graphite/LFP system. Full article
(This article belongs to the Special Issue Research on Electrolytes Used in Energy Storage Systems)
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11 pages, 219 KiB  
Article
Altitude-Linked Distribution Patterns of Serum and Hair Mineral Elements in Healthy Yak Calves from Ganzi Prefecture
by Chenglong Xia, Yao Pan, Jianping Wu, Dengzhu Luorong, Qingting Yu, Zhicai Zuo, Yue Xie, Xiaoping Ma, Lan Lan and Hongrui Guo
Vet. Sci. 2025, 12(8), 718; https://doi.org/10.3390/vetsci12080718 (registering DOI) - 31 Jul 2025
Viewed by 55
Abstract
Mineral imbalances in livestock can critically impair growth, immunity, and productivity. Yaks inhabiting the Qinghai–Tibetan Plateau face unique environmental challenges, including high-altitude-induced nutrient variability. This study investigated the status of mineral elements and their correlations with altitude in healthy yak calves across five [...] Read more.
Mineral imbalances in livestock can critically impair growth, immunity, and productivity. Yaks inhabiting the Qinghai–Tibetan Plateau face unique environmental challenges, including high-altitude-induced nutrient variability. This study investigated the status of mineral elements and their correlations with altitude in healthy yak calves across five regions in Ganzi Prefecture, located at elevations ranging from 3100 to 4100 m. Hair and serum samples from 35 calves were analyzed for 11 essential elements (Na, K, Ca, Mg, S, Cu, Fe, Mn, Zn, Co, and Se). The results revealed widespread deficiencies. Key deficiencies were identified: hair Na and Co were significantly below references value (p < 0.05), and Se was consistently deficient across all regions, with deficiency rates ranging from 35.73% to 56.57%. Serum Mg and Cu were generally deficient (Mg deficiency > 26% above 3800 m). S, Mn (low detection), and Co were also suboptimal. Serum selenium deficiency was notably severe in lower-altitude areas (≤59.07%). Significant correlations with altitude were observed: hair sodium levels decreased with increasing altitude (r = −0.72), while hair manganese (r = 0.88) and cobalt (r = 0.65) levels increased. Serum magnesium deficiency became more pronounced at higher elevations (r = 0.58), whereas selenium deficiency in serum was more severe at lower altitudes (r = −0.61). These findings indicate prevalent multi-element deficiencies in yak calves that are closely linked to altitude and are potentially influenced by soil mineral composition and feeding practices, as suggested by previous studies. The study underscores the urgent need for region-specific nutritional standards and altitude-adapted mineral supplementation strategies to support optimal yak health and development. Full article
(This article belongs to the Section Anatomy, Histology and Pathology)
9 pages, 1703 KiB  
Article
Plasma/Serum Electrolyte and Metabolite Testing on Blood Gas Analyzer ABL837, a New Application
by Vera Y. Chen, Rachel Fullarton and Yu Chen
Diagnostics 2025, 15(15), 1923; https://doi.org/10.3390/diagnostics15151923 - 31 Jul 2025
Viewed by 73
Abstract
Background: Core laboratory chemistry analyzers typically use plasma and serum samples, while blood gas instruments use whole blood for electrolyte and metabolite tests. Due to high costs to back up the core lab chemistry analyzers, especially in the remote small community hospitals, [...] Read more.
Background: Core laboratory chemistry analyzers typically use plasma and serum samples, while blood gas instruments use whole blood for electrolyte and metabolite tests. Due to high costs to back up the core lab chemistry analyzers, especially in the remote small community hospitals, we have verified the interchangeability of serum/plasma electrolytes and metabolites on blood gas instruments (GEM4000 and Radiometer ABL90) vs. chemistry analyzers. In this study, we sought to extend the investigation to another blood gas device—Radiometer ABL837. Methods: One plasma separator tube and one serum separator tube were drawn from 20 apparently healthy individuals and outpatients and 20 intensive care unit patients. All the samples were run on Roche Cobas8000, and then were run on three Radiometer ABL837 analyzers for sodium (Na+), potassium (K+), chloride (Cl), glucose, lactate (plasma only), and creatinine parameters. Paired measurements between the ABL837 and Cobas8000 were compared, and their difference were assessed for statistical and clinical significance. Results: ABL837 demonstrated statistical significance (p < 0.05) vs. Cobas8000 on all the plasma and serum parameters. However, no parameter differences were found when comparing the plasma/serum results on ABL837 to those on Cobas8000, indicating that none were clinically significant. ABL837 also demonstrated good–excellent correlations with Cobas8000 on all the parameters. Conclusions: When comparing metabolite and electrolyte values with plasma and serum sample types, the ABL837 blood gas instruments and Cobas 8000 chemistry analyzer are interchangeable. These data proves that ABL837 can be used as a backup for a chemistry analyzer in measuring plasma and serum electrolyte and metabolite concentrations. Full article
(This article belongs to the Special Issue Recent Advances in Clinical Biochemistry)
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14 pages, 2265 KiB  
Communication
Bioelectrical Impedance Assessment in a Patient with Breast Cancer: A Case Report on the Effect of Integrative Therapies on Cellular Homeostasis
by Graziella Marino, Giovanni Pace, Lucia Sabato, Marzia Sichetti and Marisabel Mecca
Nutrients 2025, 17(15), 2506; https://doi.org/10.3390/nu17152506 - 30 Jul 2025
Viewed by 101
Abstract
Background/Objectives: Since breast cancer (BC) survival rates have increased to 91% at 5 years and 80% at 15 years postdiagnosis, there is a growing awareness of the importance of addressing the long-term well-being of patients. Consequently, integrative oncology, which combines standard therapies [...] Read more.
Background/Objectives: Since breast cancer (BC) survival rates have increased to 91% at 5 years and 80% at 15 years postdiagnosis, there is a growing awareness of the importance of addressing the long-term well-being of patients. Consequently, integrative oncology, which combines standard therapies with complementary approaches (nutrition, mind–body practices, and lifestyle modifications), has emerged as a patient-centred model aimed at improving symptom management, treatment adherence, and overall quality of life (QoL). This study aims to demonstrate how integrative therapies can benefit body composition, phase angle, and fluid and electrolyte balance through bioelectrical impedance analysis (BIA). Methods: This study considers a patient who underwent BC surgery and was enrolled in the AMICO clinic for anamnesis, as well as their oncological pathology data, assessment of QoL, and BIA. The breast surgeon specialising in integrative oncology therapies prescribed the patient curcumin and polydatin, moderate physical activity, a balanced diet, and Qigong sessions. The patient underwent monitoring through haematochemical analysis, BIA, and a QoL questionnaire, with follow-up every four months. Results: Between 4 and 12 months, fat mass (FM) and body mass index (BMI) markedly decreased, whereas fat-free mass (FFM), total body water (TBW), and skeletal muscle mass (SMM) increased progressively. Moreover, the improvements in the Na/K ratio and phase angle (PhA) suggest a shift toward better electrolyte and fluid balance and enhanced cellular integrity and membrane function. Equally outstanding were her psychological benefits in terms of mood, sleep, anxiety, and melancholy. Conclusions: Patient progress in body composition, metabolic function, pain management, and psychological status measured during the 12-month follow-up demonstrates the potential benefits of an integrative approach to supportive cancer care. Full article
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41 pages, 7932 KiB  
Article
Element Mobility in a Metasomatic System with IOCG Mineralization Metamorphosed at Granulite Facies: The Bondy Gneiss Complex, Grenville Province, Canada
by Olivier Blein and Louise Corriveau
Minerals 2025, 15(8), 803; https://doi.org/10.3390/min15080803 - 30 Jul 2025
Viewed by 101
Abstract
In the absence of appropriate tools and a knowledge base for exploring high-grade metamorphic terrains, felsic gneiss complexes at granulite facies have long been considered barren and have remained undermapped and understudied. This was the case of the Bondy gneiss complex in the [...] Read more.
In the absence of appropriate tools and a knowledge base for exploring high-grade metamorphic terrains, felsic gneiss complexes at granulite facies have long been considered barren and have remained undermapped and understudied. This was the case of the Bondy gneiss complex in the southwestern Grenville Province of Canada which consists of 1.39–1.35 Ga volcanic and plutonic rocks metamorphosed under granulite facies conditions at 1.19 Ga. Iron oxide–apatite and Cu-Ag-Au mineral occurrences occur among gneisses rich in biotite, cordierite, garnet, K-feldspar, orthopyroxene and/or sillimanite-rich gneisses, plagioclase-cordierite-orthopyroxene white gneisses, magnetite-garnet-rich gneisses, garnetites, hyperaluminous sillimanite-pyrite-quartz gneisses, phlogopite-sillimanite gneisses, and tourmalinites. Petrological and geochemical studies indicate that the precursors of these gneisses are altered volcanic and volcaniclastic rocks with attributes of pre-metamorphic Na, Ca-Fe, K-Fe, K, chloritic, argillic, phyllic, advanced argillic and skarn alteration. The nature of these hydrothermal rocks and the ore deposit model that best represents them are further investigated herein through lithogeochemistry. The lithofacies mineralized in Cu (±Au, Ag, Zn) are distinguished by the presence of garnet, magnetite and zircon, and exhibit pronounced enrichment in Fe, Mg, HREE and Zr relative to the least-altered rocks. In discrimination diagrams, the metamorphosed mineral system is demonstrated to exhibit the diagnostic attributes of, and is interpreted as, a metasomatic iron and alkali-calcic (MIAC) mineral system with iron oxide–apatite (IOA) and iron oxide copper–gold (IOCG) mineralization that evolves toward an epithermal cap. This contribution demonstrates that alteration facies diagnostic of MIAC systems and their IOCG and IOA mineralization remain diagnostic even after high-grade metamorphism. Exploration strategies can thus use the lithogeochemical footprint and the distribution and types of alteration facies observed as pathfinders for the facies-specific deposit types of MIAC systems. Full article
(This article belongs to the Section Mineral Deposits)
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18 pages, 5970 KiB  
Article
Isotonic Protein Solution Supplementation Enhances Growth Performance, Intestinal Immunity, and Beneficial Microbiota in Suckling Piglets
by Changliang Gong, Zhuohang Hao, Xinyi Liao, Robert J. Collier, Yao Xiao, Yongju Zhao and Xiaochuan Chen
Vet. Sci. 2025, 12(8), 715; https://doi.org/10.3390/vetsci12080715 - 30 Jul 2025
Viewed by 188
Abstract
Suckling is crucial for piglet intestinal development and gut health, as it improves resilience during the challenging weaning phase and promotes subsequent growth. IPS, comprising Na+/K+ ions, whey protein, and glucose, has been shown to have positive effects on animal [...] Read more.
Suckling is crucial for piglet intestinal development and gut health, as it improves resilience during the challenging weaning phase and promotes subsequent growth. IPS, comprising Na+/K+ ions, whey protein, and glucose, has been shown to have positive effects on animal growth and intestinal health. The objectives of this study were to assess the impact of IPS consumption on the growth performance, immunity, intestinal growth and development, and microbiota structure of suckling piglets. A total of 160 newborn piglets were randomly divided into control and IPS groups, with IPS supplementation starting from 2 to 8 days after birth and continuing until 3 days before weaning. The findings revealed that IPS boosted the body weight at 24 days by 3.6% (p < 0.05) and improved the body weight gain from 16 to 24 days by 15.7% (p < 0.05). Additionally, the jejunal villus height and villus height to crypt depth ratio in the IPS group were notably increased to 1.08 and 1.31 times (p < 0.05), respectively, compared to the control group. Furthermore, IPS elevated the plasma levels of IgA and IgM, reduced the plasma levels of blood urea nitrogen (BUN), and enhanced the content of secretory immunoglobulin A (SIgA) in the jejunal mucosa of suckling piglets. Furthermore, IPS upregulated the mRNA expression of tight junction proteins GLP-2, ZO-1, and Claudin-1 in jejunal tissue, while downregulating the regulatory genes in the Toll-like pathway, including MyD88 and TLR-4 (p < 0.05). The analysis of gut microbiota indicated that IPS altered the relative abundance of gut microbes, with an increase in beneficial bacteria like Alloprevotella and Bacteroides. In conclusion, this study demonstrates that IPS supplementation enhances weaning weight, growth performance, immune function, and intestinal development in piglets, supporting the integration of IPS supplementation in the management of pre-weaning piglets. Full article
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21 pages, 4014 KiB  
Article
Optimized Mortar Formulations for 3D Printing: A Rheological Study of Cementitious Pastes Incorporating Potassium-Rich Biomass Fly Ash Wastes
by Raúl Vico Lujano, Luis Pérez Villarejo, Rui Miguel Novais, Pilar Hidalgo Torrano, João Batista Rodrigues Neto and João A. Labrincha
Materials 2025, 18(15), 3564; https://doi.org/10.3390/ma18153564 - 30 Jul 2025
Viewed by 205
Abstract
The use of 3D printing holds significant promise to transform the construction industry by enabling automation and customization, although key challenges remain—particularly the control of fresh-state rheology. This study presents a novel formulation that combines potassium-rich biomass fly ash (BFAK) with an air-entraining [...] Read more.
The use of 3D printing holds significant promise to transform the construction industry by enabling automation and customization, although key challenges remain—particularly the control of fresh-state rheology. This study presents a novel formulation that combines potassium-rich biomass fly ash (BFAK) with an air-entraining plasticizer (APA) to optimize the rheological behavior, hydration kinetics, and structural performance of mortars tailored for extrusion-based 3D printing. The results demonstrate that BFAK enhances the yield stress and thixotropy increases, contributing to improved structural stability after extrusion. In parallel, the APA adjusts the viscosity and facilitates material flow through the nozzle. Isothermal calorimetry reveals that BFAK modifies the hydration kinetics, increasing the intensity and delaying the occurrence of the main hydration peak due to the formation of secondary sulfate phases such as Aphthitalite [(K3Na(SO4)2)]. This behavior leads to an extended setting time, which can be modulated by APA to ensure a controlled processing window. Flowability tests show that BFAK reduces the spread diameter, improving cohesion without causing excessive dispersion. Calibration cylinder tests confirm that the formulation with 1.5% APA and 2% BFAK achieves the maximum printable height (35 cm), reflecting superior buildability and load-bearing capacity. These findings underscore the novelty of combining BFAK and APA as a strategy to overcome current rheological limitations in digital construction. The synergistic effect between both additives provides tailored fresh-state properties and structural reliability, advancing the development of a sustainable SMC and printable cementitious materials. Full article
(This article belongs to the Section Construction and Building Materials)
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15 pages, 288 KiB  
Article
Association of Dietary Sodium-to-Potassium Ratio with Nutritional Composition, Micronutrient Intake, and Diet Quality in Brazilian Industrial Workers
by Anissa Melo Souza, Ingrid Wilza Leal Bezerra, Karina Gomes Torres, Gabriela Santana Pereira, Raiane Medeiros Costa and Antonio Gouveia Oliveira
Nutrients 2025, 17(15), 2483; https://doi.org/10.3390/nu17152483 - 29 Jul 2025
Viewed by 186
Abstract
Introduction: The sodium-to-potassium (Na:K) ratio in the diet is a critical biomarker for cardiovascular and metabolic health, yet global adherence to recommended levels remains poor. Objectives: The objective of this study was to identify dietary determinants of the dietary Na:K ratio and its [...] Read more.
Introduction: The sodium-to-potassium (Na:K) ratio in the diet is a critical biomarker for cardiovascular and metabolic health, yet global adherence to recommended levels remains poor. Objectives: The objective of this study was to identify dietary determinants of the dietary Na:K ratio and its associations with micronutrient intake and diet quality. Methods: An observational cross-sectional survey was conducted in a representative sample of manufacturing workers through a combined stratified proportional and two-stage probability sampling plan, with strata defined by company size and industrial sector from the state of Rio Grande do Norte, Brazil. Dietary intake was assessed using 24 h recalls via the Multiple Pass Method, with Na:K ratios calculated from quantified food composition data. Diet quality was assessed with the Diet Quality Index-International (DQI-I). Multiple linear regression was used to analyze associations of Na:K ratio with the study variables. Results: The survey was conducted in the state of Rio Grande do Norte, Brazil, in 921 randomly selected manufacturing workers. The sample mean age was 38.2 ± 10.7 years, 55.9% males, mean BMI 27.2 ± 4.80 kg/m2. The mean Na:K ratio was 1.97 ± 0.86, with only 0.54% of participants meeting the WHO recommended target (<0.57). Fast food (+3.29 mg/mg per serving, p < 0.001), rice, bread, and red meat significantly increased the ratio, while fruits (−0.16 mg/mg), dairy, white meat, and coffee were protective. Higher Na:K ratios were associated with lower intake of calcium, magnesium, phosphorus, and vitamins C, D, and E, as well as poorer diet quality (DQI-I score: −0.026 per 1 mg/mg increase, p < 0.001). Conclusions: These findings highlight the critical role of processed foods in elevating Na:K ratios and the potential for dietary modifications to improve both electrolyte balance and micronutrient adequacy in industrial workers. The study underscores the need for workplace interventions that simultaneously address sodium reduction, potassium enhancement, and overall diet quality improvement tailored to socioeconomic and cultural contexts, a triple approach not previously tested in intervention studies. Future studies should further investigate nutritional consequences of imbalanced Na:K intake. Full article
(This article belongs to the Special Issue Mineral Nutrition on Human Health and Disease)
25 pages, 15689 KiB  
Article
Mineralogical and Chemical Properties and REE Content of Bauxites in the Seydişehir (Konya, Türkiye) Region
by Muazzez Çelik Karakaya and Necati Karakaya
Minerals 2025, 15(8), 798; https://doi.org/10.3390/min15080798 (registering DOI) - 29 Jul 2025
Viewed by 270
Abstract
The most important bauxite deposits in Türkiye are located in the Seydişehir (Konya) and Akseki (Antalya) regions, situated along the western Taurus Mountain, with a total reserve of approximately 44 million tons. Some of the bauxite deposits have been exploited for alumina since [...] Read more.
The most important bauxite deposits in Türkiye are located in the Seydişehir (Konya) and Akseki (Antalya) regions, situated along the western Taurus Mountain, with a total reserve of approximately 44 million tons. Some of the bauxite deposits have been exploited for alumina since the 1970s. In this study, bauxite samples, collected from six different deposits were examined to determine their mineralogical and chemical composition, as well as their REE content, with the aim of identifying which bauxite types are enriched in REEs and assessing their economic potential. The samples included massive, oolitic, and brecciated bauxite types, which were analyzed using optical microscopy, X-ray diffraction (XRD), X-ray fluorescence (XRF) and inductive coupled plasma-mass spectrometry (ICP-MS), field emission scanning electron microscopy (FESEM-EDX), and electron probe micro-analysis (EPMA). Massive bauxites were found to be more homogeneous in both mineralogical and chemical composition, predominantly composed of diaspore, boehmite, and rare gibbsite. Hematite is the most abundant iron oxide mineral in all bauxites, while goethite, rutile, and anatase occur in smaller quantities. Quartz, feldspar, kaolinite, dolomite, and pyrite were specifically determined in brecciated bauxites. Average oxide contents were determined as 52.94% Al2O3, 18.21% Fe2O3, 7.04% TiO2, and 2.69% SiO2. Na2O, K2O, and MgO values are typically below 0.5%, while CaO averages 3.54%. The total REE content of the bauxites ranged from 161 to 4072 ppm, with an average of 723 ppm. Oolitic-massive bauxites exhibit the highest REE enrichment. Cerium (Ce) was the most abundant REE, ranging from 87 to 453 ppm (avg. 218 ppm), followed by lanthanum (La), which reached up to 2561 ppm in some of the massive bauxite samples. LREEs such as La, Ce, Pr, and Nd were notably enriched compared to HREEs. The lack of a positive correlation between REEs and major element oxides, as well as with their occurrences in distinct association with Al- and Fe-oxides-hydroxides based on FESEM-EDS and EPMA analyses, suggests that the REEs are present as discrete mineral phases. Furthermore, these findings indicate that the REEs are not incorporated into the crystal structures of other minerals through isomorphic substitution or adsorption. Full article
(This article belongs to the Special Issue Critical Metal Minerals, 2nd Edition)
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