Search Results (144)

This study aims to assess the sustainability of an agroforestry system integrating Schima wallichii (puspa) and Amomum cardamomum (cardamom) within the Gunung Walat Educational Forest—IPB University Educational Forest (HPGW), Indonesia, by applying the RAP-AFS (Rapid Appraisal Technique for Evaluating Agroforestry Sustainability) approach. The assessment was conducted across four key dimensions: ecological, economic, social, and institutional. The findings suggest that the overall sustainability of the agroforestry system in HPGW is moderately good, with a sustainability index value of 55.94. The social and ecological dimensions performed particularly well, scoring 67.51 and 61.43, respectively. The economic dimension achieved an index score of 55.45, although price fluctuations and reliance on middlemen continue to pose challenges. Meanwhile, the institutional dimension showed the lowest performance, with an index value of 39.37, underscoring the need to enhance institutional support, capacity building, and extension services to strengthen sustainability. In light of these findings, it is recommended to prioritize strengthening institutional frameworks, stabilizing market prices, and expanding soil conservation practices, alongside promoting greater social participation, to ensure the long-term sustainability of the agroforestry system in this region. Full article

Background: Evidence suggests that physical activity and fitness tend to decline during the transition to higher education, yet the influence of institutional environment on these variables remains unclear. Objectives: This study aimed to compare physical fitness and physical activity levels between first-year students from two higher education institutions within the same national context. Methods: Fifty-eight male university students (IPB (Instituto Politécnico de Bragança): n = 31; IPG (Instituto Politécnico da Guarda): n = 27; mean age IPB = 19.2 ± 1.8 years; IPG = 19.8 ± 5.5 years) were assessed for body composition, handgrip strength, mid-thigh pull, standing long jump, stork balance, flexibility, and estimated VO_2max (StepTest4all). Physical activity levels were determined using the International Physical Activity Questionnaire (IPAQ) and were classified as low, moderate, or high. Between-group comparisons were performed using independent samples t-tests, with statistical significance set at p < 0.05. Results: Students from IPB presented significantly higher IPAQ scores (2.97 ± 0.18) compared with those from IPG (2.56 ± 0.64; t = 3.235, p = 0.003, d = 0.90), along with superior standing long jump performance (1.95 ± 0.15 m vs. 2.12 ± 0.24 m; t = −3.239, p = 0.002, d = 0.85). No significant differences were observed for body composition, strength, flexibility, balance, psychological well-being or VO_2max (all p > 0.05), although small effects were noted for flexibility (d = 0.50) and VO_2max (d = 0.48). The distribution of physical activity categories revealed that 96.8% of IPB students were classified as highly active, whereas IPG students were more evenly distributed across high (63.0%), moderate (28.6%), and low (7.4%) activity levels. Conclusions: These findings indicate that institutional environment and access to exercise opportunities may influence physical activity behavior and lower-limb power in university students. Promoting structured physical activity programs and recreational opportunities within higher education may help sustain adequate fitness and health in this population. Full article

Background: Rhodiola quadrifida (Pall.) Fisch. & C.A. Mey. (Crassulaceae) is a rare medicinal species in the Kazakh Altai, yet information on its population structure and genetic diversity remains limited. This study presents findings from an investigation of natural R. quadrifida populations. Methods: The morphometric characteristics, population age structure, and genetic diversity of the plants were analysed using PCR-based genome profiling. Genetic diversity within R. quadrifida populations was assessed using PCR primers for binding sites complementary to a specific region at the 3′ terminus of a particular tRNA. Results: The populations exhibited variations in morphological traits, age structures, and reproductive strategies. The IVA-1, IVA-2 and KOK populations represent the most mature stages, characterized by a dominance of vegetative reproduction and a disturbed age structure, with a predominance of senile and virgin individuals. In contrast, the LIN-1 and LIN-2 population is characterized by a balanced age structure, encompassing all ontogenetic groups, and a mixed reproductive system that includes both sexual and vegetative propagation. Genetic diversity, as measured by Shannon’s information index, ranged from 0.194 to 0.247, indicating low genetic diversity in R. quadrifida. Analysis of molecular variance (AMOVA) revealed significantly greater variation within populations (62%) than among populations (38%). Genetic diversity was higher in the LIN-1 and LIN-2 populations, which employs a mixed reproductive system (clonal and seeds), than in populations dominated by vegetative reproduction. Both LIN populations, characterized by a mixed reproductive system, exhibited higher genetic diversity than the KOK, IVA-1 and IVA-2 populations, where vegetative reproduction predominated. Conclusions: These results underscore the necessity for priority conservation measures, including monitoring population size and age structure in populations with low levels of seed reproduction and disturbed age structure. Additional measures include supporting in situ and ex situ conservation, such as clonal collection, seed banks, and tissue cultures, as well as restricting the harvesting of medicinal raw materials. Full article

The increasing demand for sustainable construction materials has underscored the limitations of conventional interlocking paving blocks (IPBs), particularly regarding durability, mechanical performance, and environmental impact. To overcome these shortcomings, this study proposes an integrated strategy of incorporating various waste materials in the production of IPBs namely: Untreated and surface-treated crumb rubber (CR) as a partial sand replacement at levels of 10%, and 20%; ceramic powder (CP) and glass powder (GP) as cement partial replacement at levels of 10%, 20%, and 30%, recycled ceramic as a full replacement of dolomite; and discrete fibers (basalt, polypropylene, and glass). A series of experimental tests was conducted to assess the slump, compressive and flexural strengths, water absorption, abrasion resistance, and microstructure of the proposed IPBs. The results of this study revealed that while untreated CR reduced workability and strength, it enhanced flexural resistance. Surface treatments of CR using CP and GP improved bonding and reduced porosity, with 20% CP yielding the best performances of 17.3% and 20% increases in compressive and flexural strength, respectively. Among fibers, 0.6% basalt fiber offered optimal strength and abrasion resistance (0.20 mm), while 0.6% polypropylene fiber achieved the lowest water absorption (3.70%) and a minimum abrasion depth of 0.28 mm at TR20CP mix. Microstructure analyses confirmed denser microstructure and stronger interfacial bonding in treated and fiber-reinforced mixes. This work offers a scalable, waste-based enhancement strategy for producing more durable and sustainable production of IPBs. Full article

Analysis of the genetic diversity of natural populations of economically valuable plants is important for conservation and selection strategies. In this study, the genetic diversity of 11 natural populations of Hippophae rhamnoides L.—sea buckthorn from different regions of Kazakhstan—was studied using Inter-Primer Binding Site Polymorphism (iPBS) markers based on conserved sequences of tRNA primer-binding sites (PBSs) that initiate retrotransposon replication. Universal PBS primers yielded reproducible and informative amplicons, forming unique profiles for each sample. Analysis of molecular variance showed that 60% of the total genetic variation was due to intrapopulation differences and 40% was due to interpopulation differentiation. The highest genetic diversity was found in the Shetlasty and Tersayryk sea buckthorn populations, whereas the Karatal and Topkain populations were characterised by minimal values, although unique alleles were observed in the latter population, indicating possible adaptation to local environmental conditions or genetic isolation. Principal coordinate analysis, UPGMA clustering, and Bayesian structure analysis (K = 4) confirmed geographical structuring. This study provides insights into the genetic structure of sea buckthorn populations in Kazakhstan and demonstrates the effectiveness of iPBS markers for assessing intraspecific diversity. The obtained results provide a basis for the conversation of H. rhamnoides gene pool and underscore the need for both in situ conservation of genetically rich populations and ex situ protection of vulnerable groups. Full article

Modifying lubricants with hard material particles improves lubricant performance by allowing the particles to penetrate the contact area and separate the contacting surfaces. The use of solid particles as additives in fluid lubricants presents a promising avenue for providing effective lubrication under high loads in sheet metal forming. This article presents the results of friction tests using the bending under tension friction tribotester. Low-carbon DC01 steel sheets were used as the test material. The main goal of the study was to determine the effect of lubricant modification by adding MoS₂ and SiO₂ particles and the modification of 145Cr6 steel countersamples on the coefficient of friction (CoF), changes in friction-induced surface roughness and friction mechanisms. The surfaces of the countersamples were modified using electron beam melting and the ion implantation of lead (IPb). It was found that increasing the SiO₂ and MoS₂ content in DC01/145Cr6 and DC01/IPb contacts under base oil lubrication conditions resulted in a decrease in the CoF value. For the countersample subjected to electron beam melting, considering all friction conditions, the CoF decreased between 31.9% and 37.5%. Full article

This study investigated interspecific and intraspecific polymorphism in Rheum (Polygonaceae) from Kazakhstan using the inter-primer binding site (iPBS) retrotransposon marker system. The results revealed considerable variation in the level and nature of genetic polymorphism both within and among Rheum species and ecopopulations across different regions of Kazakhstan. Rh. compactum and the ecopopulation Rh. tataricum from the Zhambyl Region (ZH) exhibited the lowest levels of polymorphism, supporting their designation as conservation priorities. Genetic differentiation analysis among species and ecopopulations identified clear distinctions, resulting in the formation of well-defined clusters with high bootstrap support. Minimal genetic distances were observed between the two ecopopulations of Rh. tataricum, along with a high degree of intraspecific genetic homogeneity in Rh. compactum and Rh. nanum. A distinct genetic divergence between Rh. compactum and the other taxa was detected, reinforcing its status as a separate species rather than a synonym of Rh. altaicum. The iPBS markers proved effective for investigating genetic variation in Rheum, offering valuable insights for future studies aimed at understanding the evolutionary history of the genus. Full article

Protected areas (PAs) are increasingly exposed to anthropogenic pressures under global change scenarios, with surrounding land subdivision and land use/land cover change (LULCC) dynamics often undermining their conservation goals and intensifying demand for basic services such as water availability. This study analyzed the buffer zone around Cerro Castillo National Park in Chilean Patagonia to assess the evolution of rural private properties, considering their subdivision, LULCC, and legal water demand dynamics. Using cadastral records, Landsat 8 imagery, and official water rights databases, we quantified property subdivision and analyzed LULCC and water rights distribution patterns through spatial overlap analysis. Results indicate a nearly fourfold increase in subdivisions between 2011 and 2023, with 304 properties divided into 3237 units occupying 43.7% of the private land area. LULCC analysis revealed a net recovery of native forest (+10%) alongside notable increases in urban coverage (+152%) and exotic plantations (+245%). Legal water demand almost doubled, with 68% of consumptive rights concentrated in subdivided properties. These findings highlight property subdivision as an important factor influencing socioecological change in the territories that surround PAs. We argue that subdivision dynamics can serve as an early indicator for anticipating land use pressures and can complement integrated landscape-scale planning, consistent with the transformative change approaches advocated by international biodiversity frameworks. Full article

The increasing demand for novel cut chrysanthemum cultivars has underscored the significance of precision breeding techniques, with particular emphasis on hybridization and molecular tools. This study aimed to assess the cross-compatibility of selected chrysanthemum cultivars and to evaluate the genetic, quantitative, and qualitative diversity among the resulting F₁ progenies. A total of six hybrid combinations were generated using five commercial parental cultivars. Ploidy levels were determined via flow cytometry and chromosome counting, confirming that all parents were allohexaploid (2n = 6x = 54). Pollen viability and germination rates varied significantly among male parents, influencing hybridization success. A total of 30,391 seeds were obtained, with germination rates ranging from 2.69% to 10.73%, depending on the cross combination. F₁ progenies showed considerable phenotypic variability in flowering time, flower stalk length, flower diameter, and branch weight. Molecular characterization using eight iPBS primers revealed a high polymorphism rate (93%) with a mean Polymorphism Information Content (PIC) value of 0.614, confirming substantial genetic diversity among genotypes. Cluster and principal coordinate analyses demonstrated that most F₁ genotypes grouped closely with their maternal parents, although unique genomic variations were also detected. The integration of morphological and molecular data provides valuable insights for selecting superior genotypes and optimizing breeding strategies. This study highlights the importance of evaluating hybridization potential and genetic diversity in the development of commercially viable cut chrysanthemum cultivars. Full article

Buffer zones around protected areas (PA) face complex governance challenges as territorial transitions accelerate globally, yet limited consensus exists on their definition, ecosystem service (ES) priorities, and management strategies. This study employed a three-round Delphi methodology with 23 transdisciplinary experts to build consensus on buffer zone governance around Cerro Castillo National Park in Chilean Patagonia, using the IPBES ecosystem services framework to structure the analysis. Round 1 employed open-ended questions to explore expert perspectives, Round 2 evaluated 56 statements and 15 strategic components using structured questionnaires, and Round 3 refined non-consensus items. Experts achieved 76.7% overall consensus across three thematic areas: PA interface conceptualization (79.2% consensus on 24 statements), ES assessment (91.2% consensus on 34 statements), and territorial transition strategies (15 components evaluated). Water-related services achieved unanimous agreement across multiple IPBES categories, revealing their potential as boundary objects bridging conservation and development perspectives. Educational approaches and voluntary compliance emerged as high-feasibility strategic components, while regulatory frameworks showed high importance but implementation uncertainty. The study demonstrates that structured expert consultation can identify collaborative pathways for buffer zone governance, with water services providing concrete entry points for multi-stakeholder cooperation and education-based strategies offering promising implementation pathways for sustainable territorial transitions. Full article

SARS-CoV-2 entry into host cells involves multiple steps and is a highly orchestrated process. Both the host protease TMPRSS2 and the HR1/HR2 segment within the spike (S) protein play a crucial role in promoting viral invasion. Herein, we report a series of bifunctional SARS-CoV-2 entry inhibitors formed by covalently linking a TMPRSS2 inhibitor, Camostat (Cm), and an HR1-targeting peptide fusion inhibitor IPB19 via a poly (ethylene glycol) (PEG) linker. Among them, IP4X and IP4Z display potent inhibitory activities against SARS-CoV-2 with similar IC₅₀ values of 0.16 μM and 0.17 μM, respectively. The efficacy surpassed that of their parent inhibitors by approximately 28-fold relative to Camostat and 15-fold relative to IPB19. We confirm that IP4X and IP4Z exhibit a dual-targeting mechanism by binding to both TMPRSS2 and HR1 region of S protein. These findings highlight the potential of the bifunctional inhibitors for further development as a novel multitarget therapy against SARS-CoV-2 infection and enrich the understanding of S-mediated entry of SARS-CoV-2 into host cells. Full article

The sustainability of coastal ecosystem resources hinges on collective action; however, conservation programs often fail when the underlying values that shape human behavior are overlooked. Anchored in behavioral change theory and common-pool resource governance, this study explores artisanal fishers’ subconscious value orientations as drivers of conservation. Relational value, as defined by IPBES, was used to assess the strength of the relationship between artisanal fishers and coastal resources. Principal Component Analysis of survey data revealed three value components, Natural Legacy Value (NLV), Non-Economic Value (NEV), and Economic Value (EV), and two conservation orientations, tangible and intangible. Relational valuation, blending intrinsic and instrumental motives, strongly influences conservation attitudes. NEV correlates with religion and intangible measures (knowledge, cultural practices) (R = 0.153, p < 0.05), while EV supports both tangible and intangible strategies but none of the demographic factors, indicating strategic leverage points for inclusive engagement. Conversely, NLV’s negative association with tangible measures reflects cultural sensitivities that can hinder compliance. The results suggest that embedding value-sensitive approaches into co-management frameworks can foster trust, reciprocity, and behavioral change, key elements in Ostrom’s design principles. This study contributes to sustainability science by linking socio-psychological drivers to governance strategies for promoting coastal socio-ecological systems resilience. Full article

Poly(ADP-ribose) polymerases (PARP) are a family of enzymes that were proven to play an essential role in the initiation and activation of DNA repair processes in the case of DNA single-strand breaks. The inhibition of PARP enzymes might be a promising option for the treatment of several challenging types of cancers, including triple-negative breast cancer (TNBC) and non-small cell lung carcinoma (NSCLC). This study utilizes several techniques to screen the compound collection of the Leibniz Institute of Plant Biochemistry (IPB) to identify novel hPARP-1 inhibitors. First, an in silico pharmacophore-based docking study was conducted to virtually screen compounds with potential inhibitory effects. To evaluate these compounds in vitro, a cell-free enzyme assay was developed, optimized, and employed to identify hPARP-1 inhibitors, resulting in the discovery of two novel scaffolds represented by compounds 54 and 57, with the latter being the most active one from the compound library. Furthermore, fluorescence microscopy and synergism assays were performed to investigate the cellular and nuclear pathways of hPARP-1 inhibitor 57 and its potential synergistic effect with the DNA-damaging agent temozolomide. The findings suggest that the compound requires further lead optimization to enhance its ability to target the nuclear PARP enzyme effectively. Nonetheless, this new scaffold demonstrated a five-fold higher PARP inhibitory activity at the enzyme level compared to the core structure of olaparib (OLP), phthalazin-1(2H)-one. Full article

Very high resolution (VHR) remote sensing change detection (CD) is crucial for monitoring Earth’s dynamics but faces challenges in capturing fine-grained changes and distinguishing them from pseudo-changes due to varying acquisition conditions. Existing deep learning methods often suffer from information loss via downsampling, obscuring details, and lack filter adaptability to spatial heterogeneity. To address these issues, we introduce Information-Preserving Adaptive Convolutional Network (IPACN). IPACN features a novel Information-Preserving Backbone (IPB), leveraging principles adapted from reversible networks to minimize feature degradation during hierarchical bi-temporal feature extraction, enhancing the preservation of fine spatial details, essential for accurate change delineation. Crucially, IPACN incorporates a Frequency-Adaptive Difference Enhancement Module (FADEM) that applies adaptive filtering, informed by frequency analysis concepts, directly to the bi-temporal difference features. The FADEM dynamically refines change signals based on local spectral characteristics, improving discrimination. This synergistic approach, combining high-fidelity feature preservation (IPB) with adaptive difference refinement (FADEM), yields robust change representations. Comprehensive experiments on benchmark datasets demonstrate that IPACN achieves state-of-the-art performance, showing significant improvements in F1 score and IoU, enhanced boundary delineation, and improved robustness against pseudo-changes, offering an effective solution for very high resolution remote sensing CD. Full article

The Iberian Pyrite Belt (IPB) contains the world’s largest massive sulfide deposit, and, due to extensive mining developed during the last 200 years, large amounts of mining waste have been abandoned in this area, with roasted pyrite ash being the focus of this study. Polymetallic mining is also classified as a NORM (naturally occurring radioactive material) activity, thus the main objective of this work was to develop a radiological and physicochemical characterization of this waste (mineral phases, elemental and radionuclide concentrations) in order to perform a valorization diagnosis of this material. The composition of this waste strongly depends on its origin (mine), and is mainly formed by iron oxides (hematite, Fe₂O₃) and heavy metals and metalloids such as As, Pb, Zn, and Cu, in levels 2–4 orders of magnitude higher than those of undisturbed soils, depending on each particular element. However, the average natural radionuclide levels are similar to those of unperturbed soils (around 30 Bqkg⁻¹ of ²³⁸U-series, 50 Bqkg⁻¹ of ²³²Th, and 70 Bqkg⁻¹ for ⁴⁰K), thus they are below the limits established by European Union regulations to require radiological control during their future valorization. As the main potential applications of roasted pyrite ash, the valorization diagnosis indicates that it can be used as a source of Fe (FeCl₃ or FeSO₄), or an additive in the manufacturing of cements, pigments, etc. Full article