Search Results (287)

Background/Objectives: Transarterial radioembolization (TARE) plays an increasing role in the treatment of hepatocellular carcinoma (HCC). Several isotope platforms have been introduced; however, comparative data remain limited. We aimed to compare outcomes following Holmium-166 (Ho-166) and Yttrium-90 (Y-90) TARE in HCC patients. Methods: We retrospectively analyzed a prospectively maintained cohort of HCC patients treated with Ho-166 or Y-90 TARE between 2022 and 2024. Tumor burden and treatment response were assessed using contrast-enhanced CT or MRI. Overall survival (OS) and progression-free survival (PFS) were estimated using the Kaplan–Meier method and further evaluated using multivariable Cox regression analyses adjusted for baseline confounders. Results: A total of 73 patients were analyzed, including 39 treated with Y-90 and 34 with Ho-166 TARE. Median follow-up duration was 20.9 months. No significant differences in OS or PFS were observed between treatment groups. Response outcomes, including objective response rate, complete response rate, and duration of response, were also comparable between groups. In the overall cohort, median OS was not reached in Barcelona Clinic Liver Cancer (BCLC) A or B patients and was 12.9 months in BCLC C patients. Median PFS was 20.1, 11.7, and 6.1 months in BCLC A, B, and C patients, respectively. Conclusions: Ho-166 and Y-90 TARE achieved favorable outcomes across different BCLC stages without significant differences in survival, treatment response, or duration of response between the two platforms. These findings further support the role of TARE in the management of patients with HCC. Full article

Phosphorites are a vital source of phosphorus for agricultural and industrial applications and are increasingly recognized for their potential as secondary repositories of critical raw materials (CRMs) such as rare earth elements plus yttrium (REYs). This study investigates deep-sea phosphorites from the Galicia Bank, Madeira, and Canary Seamounts, in the NE Atlantic Ocean, which are spatially associated with ferromanganese (Fe-Mn) mineralization. Through integrated petrographic, geochemical, and in situ isotopic analyses (O and Sr), we assess the timing, processes, and paleoenvironmental conditions of phosphogenesis and its implications for CRM enrichment. Rare earth element patterns in apatite reflect a predominant seawater-derived signature with variable Ce anomalies. Nevertheless, variable Y/Ho ratios point to evolving fluid sources including a hydrogenous component (directly derived from seawater), modified porewaters and, locally, volcanic or possibly hydrothermal inputs. Oxygen and strontium isotope compositions constrain phosphogenesis to several episodes ranging from the Upper Cretaceous to the Middle Miocene, with distinct isotopic shifts identifying both primary formation and later overprinting processes mostly linked to Fe-Mn oxyhydroxide growth or volcanic–hydrothermal activity. These findings highlight the dynamic and multiphase nature of phosphorite formation in deep-marine settings. The integration of high-resolution geochemical and isotopic tools proves essential for reconstructing genetic histories, defining metallogenic context and evaluating CRM prospectivity in complex submarine systems. Full article

The Dadonggou Mo deposit in Western Hebei, within the Yanshan–Liaoning Mo metallogenic belt, is a newly recognized medium-sized porphyry Mo system. Exploration has delineated 126 orebodies, most of which are blind, with identified resources of ~22,000 t Mo at an average grade of 0.071% Mo. Integrated lithogeochemistry, zircon U-Pb chronology, molybdenite Re-Os geochronology, quartz fluid-inclusion microthermometry, and H-O-S isotope analyses constrain the mineralization age, ore-fluid evolution, and sources of ore-forming materials. The zircon U-Pb dating of the ore-bearing granite porphyry and quartz porphyry from the Dadonggou molybdenum deposit yields ages ranging from 135.8 Ma to 141.5 Ma. The low Ti content in zircons indicates that they are super-wet magmatic rocks. The magmatic evolution experienced a change in oxygen fugacity from oxidizing to reducing conditions, which facilitated the initial enrichment of molybdenum. Molybdenite yields a Re-Os isochron age of 135.9 ± 4.0 Ma and a weighted mean model age of 134.2 ± 1.6 Ma, indicating Early Cretaceous mineralization. Ore fluids evolved from an early CO₂-H₂O-NaCl system with relatively high temperature and salinity to a later H₂O-NaCl system with lower temperature and salinity. Isotopic data indicate progressive meteoric-water incorporation into dominantly magmatic fluids. Sulfur isotopes and high Re contents in molybdenite indicate a mixture of mantle magma mixed with some seawater. Lower late-stage trapping pressures record post-ore depressurization and hydrothermal-system shallowing. Full article

Monoclonal antibodies (mAbs) have been the subject of extensive study in recent years due to their recognition as highly promising therapeutic molecules offering high specificity and a low risk of side effects. Monitoring the structure of these molecules is crucial for developing new therapeutics, characterizing interactions with antigens or receptors, and explaining potential changes in activity between antibody production batches. However, commonly used biophysical approaches provide only low-spatial-resolution information, and conventional structural biology techniques such as crystallography and cryo-electron microscopy (cryo-EM) are difficult to apply to these highly dynamic proteins. Solution nuclear magnetic resonance (NMR) spectroscopy is the method of choice for structural studies of flexible proteins at atomic resolution; however, it has traditionally been limited to low-molecular-weight biological systems. In this review, we present recent advances in NMR spectroscopy and advanced isotopic labeling methods that have enabled the atomic-resolution study of both the crystallizable (Fc) and antigen-binding (Fab) fragments of antibodies. We show how NMR is becoming a powerful tool for investigating full-length mAbs at an atomic level, opening up new possibilities for the characterization and in-depth quality control of therapeutic antibodies in solution. Full article

The Dajing giant Cu–Sn polymetallic deposit is located in the Cu–Sn–Ag–Pb–Zn polymetallic belt of the southern Great Xing’an Range, NE China. Research on its ore genesis is of great significance for understanding Sn polymetallic mineralization in this region. In this study, pyrite, arsenopyrite, and sphalerite were analyzed by electron-microprobe analysis (EMPA) and in situ S–Pb isotope analysis. Previously published fluid-inclusion microthermometric and H–O isotope data were also incorporated to constrain fluid evolution and ore genesis. Both in situ S and Pb isotopic compositions fall within short ranges. The δ³⁴S values suggest a sulfur reservoir with possible magmatic contribution, whereas Pb isotopes indicate a mainly crustal Pb signature in an orogenic setting. Arsenopyrite records variations in As, S, Fe, and Co contents from core to rim. The Co-rich core shows Co enrichment accompanied by Fe depletion, consistent with Co-for-Fe isomorphous substitution. These features indicate changes in local fluid chemistry during arsenopyrite growth. Sulfur isotope geothermometry based on coexisting late-stage pyrite–sphalerite pairs yields 118–233 °C, with an average of 159 ± 49 °C, indicating medium- to low-temperature hydrothermal activity during the late sulfide stage. The Dajing deposit is interpreted as a fault-controlled hydrothermal vein-type Cu–Sn polymetallic deposit formed in a Late Jurassic extensional setting. Ore precipitation was likely promoted by cooling during upward fluid migration away from the magmatic heat source, pressure release, meteoric-water mixing, and fluid–rock interaction with granitic rocks and Linxi Formation wall rocks. This study provides mineral-scale constraints on fluid evolution and ore genesis in the Great Xing’an metallogenic belt. Full article

The Shizui Cu–Pb–Zn deposit is located in central Jilin Province. It sits at the tectonic junction between the eastern Xing’an–Mongolia Orogenic Belt (XMOB) and the northeastern North China Craton (NCC). This is the first discovered Paleozoic Cu-polymetallic deposit in the region. Our study combines detailed geological investigation with systematic fluid inclusion analysis. We analyzed samples from four distinct paragenetic stages. Analytical methods include microthermometry, laser Raman spectroscopy, and hydrogen-oxygen isotope analysis. These data constrain the source, evolution, and precipitation mechanisms of the ore-forming fluids. The results delineate a clear evolutionary path: the ore-forming fluid originated as a high-temperature (346–437 °C), high-salinity (up to 51.68 wt.% NaCl equiv.) NaCl–H₂O–CO₂ system during the early quartz-sulfide stage (Stage I, Quartz ± Arsenopyrite ± Pyrite Stage), as evidenced by the coeval presence of high-salinity S-type and CO₂-rich C-type inclusions, indicating fluid immiscibility. The fluid then evolved into a boiling, medium temperature to high temperature (262–355 °C), high-salinity NaCl–H₂O system during the later part of early quartz-sulfide stage (Stage II, Quartz-Cu Polymetallic Sulfide Stage), a transition marked by the common coexistence of liquid-rich (L-type) and vapor-rich (V-type) inclusions with similar homogenization temperatures. This phase separation (boiling) served as the primary trigger for the massive deposition of chalcopyrite, arsenopyrite, and pyrite. Subsequently, the system cooled and diluted, transforming into a medium- to low-temperature (182–275 °C), low-salinity, partially homogeneous NaCl–H₂O system in the late quartz-sulfide stage (Stage III, Quartz-Pb-Zn Polymetallic Sulfide Stage). Finally, in the quartz-carbonate stage (Stage IV, Quartz-Carbonate Stage), the fluid temperature further decreased, resulting in a low-temperature (128–211 °C), low-salinity, homogeneous NaCl–H₂O system. Hydrogen-oxygen isotope data show that the calculated δ¹⁸O_H2O values decreased from +6.6‰ to +6.7‰ in Stage I to +3.4‰ to +3.9‰ in Stage II, and further to −0.4‰ in Stage III, while the δD values shifted from −91.6‰ to −90.6‰, to −94.4‰ to −94.2‰, and finally to −95.7‰. This trend indicates that the initial magmatic fluid progressively mixed with meteoric water. The geological characteristics, spatial association with Hercynian biotite monzogranite, developed skarn alteration, and the documented fluid evolution trajectory collectively affirm that the Shizui deposit is a typical skarn-type system. The deposit shares significant similarities in mineralization conditions, age, and tectonic setting with the skarn-type Tianbaoshan Pb–Zn–Cu–Mo deposits in the western segment of the XarMoron–Changchun Metallogenic Belt (XCMB). This correlation strongly suggests that the Paleozoic XCMB extends eastward and holds considerable potential for the discovery of late Paleozoic skarn-type Cu-polymetallic deposits in its eastern part. Full article

The Chutuan and Jiashan fluorite deposits are situated in the Donghai–Linshu area within the southwestern segment of the Sulu ultrahigh–pressure metamorphic belt. Both deposits share similar mineralization characteristics, with fluorite veins strictly controlled by fault structures and associated with mineral assemblages comprising fluorite, barite, quartz, and calcite. Two mineralization stages have been identified in both deposits: Stage I (quartz–fluorite–barite stage), representing the main ore–forming event, and Stage II (quartz–barite–calcite stage). This study focuses on integrated geochemical and geochronological analyses of fluorite from Stage I, providing new constraints on the genesis and metallogenic processes of these deposits. Direct Sm–Nd isotopic dating of fluorite yields an isochron age of 104 ± 16 Ma, indicating that mineralization occurred during the late Early Cretaceous. Fluid inclusion and stable isotope studies reveal that the ore–forming fluids constitute a complex hydrothermal system characterized by a wide temperature range (112–324 °C) and variable salinities (0.18–21.87 wt% NaCl eq.). The H–O isotopic compositions exhibit a distinct latitudinal trend, supporting a dominant meteoric water component. However, the presence of high–temperature, high–salinity fluid inclusions, along with a shift in some δD values towards the magmatic water field, suggests episodic mixing between meteoric water and deep–seated magmatic–hydrothermal fluids. Sr–Nd isotopic data (⁸⁷Sr/⁸⁶Sr = 0.711785–0.713424; εNd(t)= −27.7 to −27.5) potentially demonstrate that the ore–forming materials (Ca and REEs) were not derived from coeval magmatic rocks. Instead, they were primarily leached from the Precambrian Donghai Group metamorphic complex through extensive water–rock interaction. Based on these findings, the Chutuan and Jiashan deposits are classified as hydrothermal vein–type systems. Fluorite precipitation was governed by a combination of fluid cooling, water–rock interaction, and fluid mixing. Finally, a metallogenic model is established, offering important insights into the genesis of fluorite mineralization in the Sulu Orogenic Belt and analogous geological settings in eastern China. Full article

The Danba–Dadu River gold belt on the western Yangtze Craton margin is a major gold province in China. The Dulanggou gold deposit is a large quartz-vein-type deposit recently discovered in this belt. Ore bodies are fault-controlled veins hosted in high-grade metamorphic rocks of the Devonian Weiguan Formation. Mineralization includes three stages: early (quartz–minor sulfide), main (quartz–abundant sulfide–native gold–Te–Bi minerals), and late (quartz–minor sulfide–calcite). Fluid inclusion studies show the following. Early-stage inclusions are mainly CO₂–H₂O-type (homogenization temperature 307–388 °C, salinity 0.4–7.1 wt.% NaCl eqv.) with minor NaCl–H₂O-type. Main-stage inclusions are dominated by CO₂–H₂O and NaCl–H₂O types, with minor pure CO₂ inclusions (homogenization temperature 207–307 °C, salinity 0.2–11.2 wt.% NaCl eqv.). Late-stage inclusions are mainly NaCl–H₂O-type (168–223 °C, 4.6–10.1 wt.% NaCl eqv.). Laser Raman analysis detects CH₄ in the fluid. The ore-forming fluid is a reducing, medium–low temperature, low-salinity H₂O–CO₂–NaCl–CH₄ system. Thermodynamic calculations of CO₂–H₂O inclusions yield total densities of 0.94–1.03 g/cm³ and total homogenization pressures of 170–276 MPa for the early stage, and slightly lower densities (0.94–1.01 g/cm³) with pressures of 170–246 MPa for the main stage, indicating a progressive pressure decrease during fluid evolution. Hydrogen and oxygen isotopes (early stage: δD −96.4‰ to −78.9‰, δ¹⁸OH₂O 6.1‰ to 6.5‰; main stage: δD −104.3‰ to −75.1‰, δ¹⁸OH₂O 5.3‰ to 7.1‰) indicate that the ore-forming fluid was mainly derived from primary magmatic water. Immiscible CO₂–H₂O and NaCl–H₂O inclusion assemblages in the main stage suggest that fluid immiscibility was the key mechanism for gold precipitation. The Dulanggou deposit resembles classic orogenic gold deposits in host rocks, ore-controlling structures, mineral assemblages, and low-salinity CO₂-rich fluids. However, its H–O isotopes and thermodynamic data point to a magmatic water source, distinct from the metamorphic water source of typical orogenic gold deposits. This highlights the diversity of fluid sources in orogenic gold systems along the western Yangtze Craton margin. Full article

Cashew apple bagasse (Anacardium occidentale L.) is an agro-industrial byproduct rich in fiber and phytochemicals, yet its effects on diet-induced fatty liver remain insufficiently characterized. This study evaluated the protective effects of cashew apple bagasse (CAB) and its hydroethanolic extract (HECAB) in rats fed a high-fat, high-carbohydrate (HFHC) diet. The proximate composition of CAB and the phenolic profile and antioxidant capacity of HECAB were characterized. Male Wistar rats were assigned to four groups and fed for 19 weeks with a standard diet, an HFHC diet, or an HFHC diet supplemented with CAB or HECAB. Anthropometric, biochemical, histological, immunohistochemical, and immunoblot analyses were performed. HECAB showed high phenolic content and marked radical-scavenging activity, and untargeted UPLC-QTOF-MS analysis yielded 12 putative secondary metabolite annotations (levels 3–4) based on accurate mass, isotope distributions, MS/MS fragmentation patterns, and predefined acceptance criteria. Relative to the standard diet group, the HFHC diet induced metabolic and hepatic alterations consistent with early-stage MASLD. Compared with HFHC, both CAB and HECAB reduced serum insulin and HOMA-IR, attenuated hepatic steatosis, increased SOD1 and CAT, and reduced NF-κB, IL-6, TNF-α, and IL-1β, whereas GPx1 remained unchanged. Both interventions also enhanced NRF2 and HO-1 compared to HFHC, with stronger nuclear positivity in the HECAB group, while CAB showed the clearest association with IL-10 restoration. These findings are consistent with modulation of antioxidant defense- and inflammatory-related pathways in early-stage MASLD and support further investigation of cashew apple bagasse as a valorized functional ingredient. However, because classical oxidative damage markers were not measured, these results should not be interpreted as direct evidence of reduced oxidative stress. In addition, the detected metabolites should be interpreted as putative annotations rather than definitive compound identifications. Full article

The Sichuan–Yunnan–Guizhou (SYG) metallogenic belt hosts numerous carbonate-hosted Pb-Zn deposits, yet the genesis of lead-dominated deposits remains poorly understood. This study investigates the Wuyi Pb deposit, a representative lead-dominated deposit in the SYG belt, through an integrated approach including field geology, fluid inclusion microthermometry, and C-H-O-S-Pb isotope geochemistry. The ore bodies occur as stratoid and lenticular lenses within the dolomitic limestone of the Ordovician Dajing Formation, controlled by both lithology and the Wuyi composite fold structure. Mineralization is divided into two stages: (I) pyrite–sphalerite–dolomite–calcite, and (II) galena–calcite–quartz–anhydrite. Fluid inclusion studies reveal that the ore-forming fluids are of the NaCl-H₂O system, characterized by moderate-low temperatures (Stage II, average 201 °C) and moderate-low salinities (Stage II, average 5.35 wt% NaCl eq.). Hydrogen and oxygen isotopes (δD = −100.97 to −76.33‰; δ¹⁸O_fluid = 7.09 to 12.10‰) indicate that the ore-forming fluids were predominantly meteoric in origin. Carbon isotopes (δ¹³C = −4.45 to 0.75‰) suggest that carbon was derived mainly from dissolution of the host carbonate rocks. Sulfur isotopes show a significant shift from Stage I (δ³⁴S = −12.40 to −3.00‰) to Stage II (δ³⁴S = −8.20 to −0.10‰ for sulfides; 25.00–29.40‰ for sulfates), indicating a transition from bacterial sulfate reduction (BSR) to thermochemical sulfate reduction (TSR) as the dominant sulfur reduction mechanism, with sulfur derived from Ordovician seawater sulfate. Lead isotopes (²⁰⁶Pb/²⁰⁴Pb = 18.10–25.37, ²⁰⁷Pb/²⁰⁴Pb = 15.50–21.72, ²⁰⁸Pb/²⁰⁴Pb = 38.29–53.90; μ = 9.30–21.05) demonstrate that metals were sourced predominantly from the Proterozoic basement rocks (Kunyang and Huili groups). Integration of geological, geochemical, and isotopic evidence indicates that the Wuyi Pb deposit formed during the Indosinian post-collisional intracontinental orogeny (ca. 230–200 Ma), when topography-driven meteoric water circulation extracted metals from the Precambrian basement and sulfur from Ordovician strata. Metal precipitation under the reduced sulfur model is caused by decreases in temperature and pressure and the water–rock reaction. This study establishes the Wuyi deposit as an MVT Pb deposit and provides a genetic model for lead-dominated mineralization in the SYG belt. Full article

Natural mineral waters hosted in volcanic terrains are globally significant, but the co-enrichment mechanisms of metasilicate and strontium remain poorly understood. Here we investigate a Jurassic volcanic-hosted mineral water source in eastern China using hydrochemical analysis, ¹⁴C dating, stable isotopes, and structural analysis. The groundwater is of Ca–Mg–HCO₃ type with slightly alkaline pH (7.44–7.63). Metasilicate (26.4–32.9 mg/L) and strontium (0.40–0.83 mg/L) co-enrichment is governed by plagioclase weathering in a bicarbonate-dominated, weakly alkaline environment where ${\mathrm{S}\mathrm{r}\mathrm{H}\mathrm{C}\mathrm{O}}_3^{+}$ ion pairs enhance strontium mobility. Pearson-corrected ¹⁴C ages of 3900–4900 years reveal that millennial-scale residence time is critical for sufficient water-rock interaction and attainment of regulatory thresholds. A conduit-barrier system formed by NW-trending extensional-shear and NNE-trending compressional-shear faults controls groundwater flow paths and residence times, leading to systematic inter-well hydrochemical differentiation. These findings provide a theoretical basis for the genetic identification, potential evaluation, and sustainable management of high-quality mineral water resources in volcanic terrains. Full article

The Dongwujiiazi deposit is a structurally controlled orogenic gold deposit situated in the eastern part of the Chifeng–Chaoyang gold belt along the northern boundary of the North China Craton. This study establishes a comprehensive metallogenic model for the Dongwujiiazi gold deposit by integrating whole-rock geochemistry (major and trace elements), in situ trace elements and REEs in zircon, multi-isotope systems (H, O, S, Pb), and precise zircon U–Pb geochronology. Five types of intrusive and associated rocks are identified within the main biotite-pyroxene gneiss host of the Dongwujiiazi gold deposit: mylonitized granitic pegmatite, mylonitized porphyritic monzogranite, propylitized fine-grained quartz monzodiorite, quartz monzonite, and porphyritic dolerite. The gold-bearing polymetallic sulfide ores are composed of pyrite, chalcopyrite, sphalerite, galena, digenite, and native gold. Zircon grains in the Dongwujiiazi gold ore (2502 ± 15 to 2539 ± 18 Ma) are inherited from surrounding Neoarchean gneiss, recording older crustal sources rather than forming contemporaneously with the gold mineralization. H–O isotopes indicate that the ore-forming fluids were mixed in origin, involving both magmatic and metamorphic components. S and Pb isotopes suggest that the mineralizing sulfur was mainly derived from a magmatic source, while lead originated predominantly from lower crustal materials associated with the surrounding high-grade metamorphic rocks. In this study, we present a new metallogenic model for the Dongwujiiazi gold deposit, in which slab-derived and lower-crustal metamorphic fluids interacted with ascending magmas, resulting in fluid mixing and gold precipitation within structurally controlled zones of gneissic host rocks. Combined geochemical and isotopic evidence (H–O, S, Pb) indicates contributions from both magmatic and metamorphic sources, supporting formation as an intracontinental orogenic gold system in an active continental margin. Full article

The northern part of the Great Xing’an Range in China hosts a prominent Au mineralization belt, where Mesozoic clastic rock-hosted Au deposits represent the mineralization type. A study of the Baoxinggou Au deposit in this region might provide new perspectives on the mineralization mechanisms of these Mesozoic clastic-rock-hosted Au deposits. This study investigated the age of mineralization, origins and evolution of the ore-forming fluids, and sources of the ore-forming materials in this deposit. Rubidium–Sr dating of sulfides yielded a mineralization age of 119 ± 2 Ma. Fluid inclusion analyses revealed that the ore precipitated from fluids with temperatures of 105–415 °C and salinities of 4.3–8.8 wt.% NaCl equivalent. Hydrogen and O isotopic data show that the ore-forming fluids were of magmatic origin and, during mineralization, the proportion of meteoric waters increased gradually and eventually dominated the late mineralization stage. Fluid mixing was the primary ore-forming mechanism. Sulfur isotopic data for pyrite and chalcopyrite (δ³⁴S_V–CDT = −4.35‰ to −0.91‰) and Pb isotopic ratios (²⁰⁶Pb/²⁰⁴Pb = 18.429–18.477; ²⁰⁷Pb/²⁰⁴Pb = 15.581–15.591) indicate the ore-forming materials were magmatic in origin, with a similar source as an Early Cretaceous diorite and mixed crust–mantle materials. The results indicate the Baoxinggou Au deposit is a magmatic–hydrothermal deposit. Full article

European pork production pursues traceability and authenticity to ensure animal welfare, food safety, and support products with geographical indications. This study reports a European survey integrating stable isotope ratios (δ¹³C, δ¹⁵N, δ³⁴S, δ¹⁸O, δ²H) and multi-element profiling using IRMS and ICP-MS, on 612 samples collected across Denmark, Poland, Italy, and Spain, with diverse production systems, breeds, feeding, and slaughter ages. Geographical and climatic gradients influenced δ²H and δ¹⁸O, which ranged from −111‰ to −89‰ in samples from Denmark and Spain and from 13.3‰ to 16.0‰ in samples from Italy and Spain, respectively. In selected farms, δ¹³C ranged from −22.7‰ to −17.0‰ depending on diet composition based on C₃ and C₄ plants. The wide variability in pig management practices suggested that δ¹⁵N (2.50 ÷ 4.96‰) increased with slaughter age and was positively correlated with Fe (3.38 ÷ 8.39 mg/kg) and Zn (9.39 ÷ 23.6 mg/kg). Most mineral components were mainly driven by feed formulation and supplementation. Principal component analysis (PCA) showed that samples were grouped based on their origin and husbandry system, confirming the key role of isotopic and elemental markers for the development of a database supporting the pork supply chains across Europe. Full article

Pacific white shrimp (Litopenaeus vannamei) is a major aquaculture product worldwide. For consumers, discriminating domestic from imported sources of shrimp meat, and individual domestic sources, can be highly desirable because of the different meat quality and environmental contamination from geographically different origins of shrimp. This study evaluated the potential of stable isotope analysis (δ¹³C, δ¹⁵N, δ²H, δ¹⁸O) with chemometric models to authenticate the origins of Pacific white shrimp sold in China. Shrimp samples from domestic (Guangxi, Fujian, Shandong, Inner Mongolia) and foreign (Ecuador) sources were analyzed, using statistical analyses. The four-isotope model achieved 89.3% cross-validation accuracy in distinguishing domestic and foreign shrimp, with an overall prediction Area Under the Curve (AUC) of 0.901 (95% CI: 0.819–0.983)—significantly outperforming single-isotope models. Differences in δ¹³C and δ¹⁵N reflected feed source variations, while δ²H and δ¹⁸O (Variable Importance in the Projection (VIP) > 1, key discriminatory indicators) mirrored geographic environmental difference. Although δ¹⁵N did not differ significantly among groups, the combination of all four isotopes reduced limitations of individual δ²H/δ¹⁸O use. This approach enhanced the precision, reliability, and applicability of stable isotope analysis for origin authentication by leveraging complementary isotopic data and robust statistical frameworks. These findings demonstrate the proposed model’s potential as a cost-effective, copyright-compliant framework for shrimp origin authentication, with implications for isotopic traceability across food science fields. Full article