Search Results (233,478)

Coronary artery bypass grafting via median sternotomy carries considerable morbidity, and minimally invasive robotic approaches have been increasingly performed for surgical revascularization of coronary arteries. We report our institutional experience in robotic-assisted minimally invasive coronary revascularization in a broad patient population with complex multivessel disease. We retrospectively reviewed robotic-assisted minimally invasive direct coronary artery bypass (RA-MIDCAB) procedures performed at our center between January 2022 and June 2026. Patient demographics, additional procedures and in-hospital outcomes were recorded. A total of 242 patients were included (mean age 63.4 ± 9.7 years; 31 (12.8%) female). Single-vessel bypass was performed in 25 (10.3%) patients; 212 (87.6%) patients underwent an operation on the arrested heart (mean cross-clamp time 66.9 ± 21.7 min) and 15 (6.2%) received an off-pump operation (mean CPB time in on-pump patients 155.7 ± 46.0 min). Full arterial revascularization was achieved in 42 (17.4%) patients; a bilateral internal mammary artery configuration was used in 9 (3.7%) patients. Coronary endarterectomy was performed in 16 (6.6%) patients and concomitant left atrial appendage (LAA) occlusion was performed in three (1.2%) patients. Epiaortic ultrasonography-guided clamp placement was performed in 27 (11.2%) patients with ascending-aortic plaque. In-hospital mortality occurred in two (0.8%) patients; no patient sustained a major neurological deficit, and the transfusion rate was 9.9%. Mean ventilation time was 4.0 (3.0–6.0) hours and mean intensive care unit stay was 22.6 ± 11.2 h. With careful planning and accumulated experience, the indications for robotic-assisted minimally invasive revascularization can be extended to include patients who require full-arterial revascularization, have ascending aortic plaques, complex coronary disease requiring endarterectomy, or atrial fibrillation where concomitant left atrial appendage occlusion is indicated. Full article

Ni–Mo alloy claddings were fabricated on low-carbon steel substrates using powder metallurgy combined with hot isostatic pressing (PM-HIP). The interfacial microstructure, elemental interdiffusion, phase composition and microhardness distribution of the bimetallic composite were investigated systematically. The results show that sound metallurgical bonding without pores, cracks and element dilution is achieved under the HIP process of 1100 °C, 120 MPa and 4 h holding time. Interdiffusion of Fe, Ni and Mo atoms forms a 20–50 μm thick interfacial transition layer, and Mo exhibits a relatively low diffusion capacity due to its large atomic radius. Two intermetallic phases, Ni₄Mo and NiMo, are formed in the cladding layer because of the inhomogeneous distribution of Mo. A prominent microhardness gradient is observed throughout the composite, and the interfacial layer presents the highest hardness of 905 HV resulting from multiple strengthening mechanisms. As an effective alternative to traditional welding and cladding technologies, the PM-HIP process exhibits great potential for manufacturing complex bimetallic components with prospective service prospects in severe corrosive environments. Full article

Atmospheric deposition in industrial basins of Central Asia is strongly influenced by local emissions and wintertime dispersion conditions. This study characterized snowpack at 63 sampling stations across Ust-Kamenogorsk, Kazakhstan, including operational background station 1, on 24–26 February 2025 after a 116-day accumulation period. Major ions were determined in a spatially distributed exploratory subset of 16 samples, and trace elements were measured in samples from all 63 stations by means of inductively coupled plasma mass spectrometry and optical emission spectrometry. Mean meltwater pH and total dissolved solids were 6.55 ± 0.34 and 37.3 ± 18.0 mg L⁻¹, respectively. Charge-balance errors for the 16 hydrochemical samples ranged from −0.3% to +0.7%. Using the contamination index based on exceedances of the current Kazakhstan water-quality thresholds, 48 stations had CI < 1, seven had CI = 1–3, and eight had CI > 3; the highest value (60.21) occurred at station 26. Principal component analysis showed that the first three components explained 53.6% of the variance and separated a broad mineral/industrial aerosol association from a Pb–Cd–Zn association consistent with non-ferrous metallurgy and mixed urban sources. Cadmium was therefore interpreted as the principal contributor to the MPC-normalized index at the most affected stations, rather than as the dominant component by absolute concentration. The dissolved fraction can be mobilized during spring melt, indicating a potential pathway to soils and receiving waters, although direct ecological or human-health risk was not quantified. Station-level point mapping and projection along the NW–SE axis showed localized multi-element maxima rather than a monotonic citywide gradient. Full article

Background: Autophagy plays a central role in maintaining cellular homeostasis, regulating growth and development, and responding to multiple stresses. Autophagy-related genes (ATGs) play critical roles in autophagy, yet their functional diversity in soybean (Glycine max) remains underexplored. Methods: Genome-wide identification of GmATG genes was performed using sequence similarity and domain-based searches against the Wm82.gnm4 reference genome, followed by characterization of physicochemical properties, chromosomal distribution, phylogenetic relationships, gene duplication, conserved motifs, gene structure, three-dimensional structural, and promoter cis-acting elements. Tissue-specific expression and multiple stresses response were examined using transcriptome data and profiled by RT-qPCR. Results: A total of 60 GmATG genes belonging to 20 subfamilies were identified in soybean. Gene family expansion was predominantly driven by fragment duplication (33 gene pairs), with the ATG8 family expanding to 12 members, and pan-genomic analysis uncovered prominent copy number variation (6–9 copies) in the ATG18 family. GmATG genes showed distinct expression patterns in response to multiple abiotic and biotic stresses. Specifically, GmATG18f was significantly induced by phosphorus deficiency in the low-phosphorus-tolerant soybean variety Nannong 94-156. GmATG8g, GmATG9d and GmATG13d showed a typical expression trend of initial increase followed by decrease, with expression levels peaking at 6–12 h after salt stress treatment. GmATG8g and GmATG9d were rapidly upregulated at the early drought stress stage, while GmATG13a maintained sustained upregulation. In response to Phomopsis stem rot, GmATG7a/8h/8i/11/13d/18e/18f displayed differential expression in resistant and susceptible soybean materials. Conclusions: This study systematically characterizes the composition, expansion and stress response patterns of the GmATG gene family, revealing functional differentiation among family members. The identified key candidate genes, including abiotic-stress-regulated GmATG8g/9d/13d/18f and biotic-stress-regulated GmATG7a/8h/8i/11/13d/18e/18f, provide valuable genetic resources for the molecular breeding of stress-tolerant soybean. Full article

The efficient development of heavy oil reservoirs is challenged by the high viscosity and poor mobility of heavy oil. Although multicomponent thermal fluid technologies involving heat, gas, and chemical agents have demonstrated potential advantages over conventional steam-based recovery methods, the microscopic synergistic mechanisms responsible for viscosity reduction remain insufficiently understood. Therefore, this study investigates the synergistic mechanisms by which heat, an alkane solvent (C₁₁H₂₄), and CO₂ reduce heavy-oil viscosity. Heavy oil from the Shengli Oilfield was selected as the research object, and rheological experiments were combined with molecular dynamics simulations to systematically analyze viscosity variations and their underlying microscopic mechanisms under different conditions. The experimental results demonstrate that increasing temperature significantly reduces heavy oil viscosity, and a characteristic transition in viscosity reduction behavior occurs at approximately 100 °C. At 90 °C, the addition 5 wt% oil-soluble viscosity reducer C₁₁H₂₄ decreases the heavy oil viscosity to 442.2 mPa·s, corresponding to a reduction rate of 83%. The solubility of CO₂ increases markedly with pressure, and at 30 MPa, the viscosity reduction exceeds 99%. The combined effects of these three factors exhibit superior viscosity-reduction performance. Molecular dynamics simulation results indicate that CO₂ and the viscosity reducer synergistically weaken the π-π stacking interactions of asphaltenes and resins in heavy oil, transforming heavy components from locally aggregated states into more uniformly dispersed configurations. Meanwhile, the intermolecular interaction energy and cohesive energy density decrease, indicating weakened molecular interactions and enhanced diffusion behavior. These results demonstrate that the synergistic viscosity-reduction mechanism of heat–gas–agent systems is mainly associated with structural disaggregation, interaction weakening, and diffusion enhancement. This study provides molecular-level insights into multicomponent thermal fluid-assisted heavy oil recovery and offers theoretical support for improving heavy oil development efficiency. Full article

Poly(β-L-malic acid) (PMLA) is a water-soluble, biodegradable aliphatic polyester whose pendant carboxyl groups support chemical functionalization for biomedical, packaging, and materials applications. Microbial fermentation can use pure sugars and biomass-derived carbon sources under mild conditions, but industrial translation remains constrained by feedstock cost and variability, strain performance, oxygen and pH control, pretreatment-derived inhibitors, and downstream recovery. This review therefore focuses on the fermentative production of PMLA from refined and renewable carbon sources, the microorganisms and metabolic routes involved, and the process variables that govern titer, yield, productivity, molecular weight, and purification. Agricultural and forestry feedstocks are compared according to their actual carbohydrate class and processing requirements. Bamboo shoot shell hydrolysate is treated as an emerging case study rather than an established production platform: one accepted shake-flask study directly demonstrated PMLA production by Aureobasidium pullulans NRRL Y-2311-1, but controlled bioreactor validation, reproducibility, techno-economic analysis, and application-specific product qualification remain to be further investigated. The review also examines autohydrolysis, low-molecular-weight PMLA for biomedical use, furan inhibition, membrane and ion-exchange purification, and the limits of current economic comparisons. This evidence-based framing identifies where bamboo-processing residues may contribute to renewable PMLA production while distinguishing laboratory feasibility from industrial readiness. Full article

Gastric cancer is one of the malignancies with the highest incidence and mortality worldwide. Helicobacter pylori (H. pylori) infection is the primary driving factor in its development. The progression of gastric mucosal malignancy follows the Correa cascade model: “chronic non-atrophic gastritis → chronic atrophic gastritis (CAG) → intestinal metaplasia (IM) → dysplasia (Dys) → gastric cancer.” Currently, clinical management faces major challenges, including increasing antibiotic resistance in H. pylori, limited pharmacological options for gastric precancerous lesions, and treatment resistance and toxicity in established gastric cancer. This review synthesizes current evidence on BBR, COP, EPI, PAL, and JAT and organizes their reported actions into a three-tier intervention framework. At the first tier, etiologic and inflammatory interception, individual alkaloids suppress H. pylori persistence through direct antibacterial injury, urease inhibition, and modulation of bacterial virulence and antibiotic susceptibility, while attenuating infection-driven inflammatory and immune responses. At the second tier, modulation of precancerous mucosal progression, preclinical studies indicate that these compounds can ameliorate gastric glandular injury and may attenuate biological processes associated with progression toward intestinal metaplasia and dysplasia. At the third tier, antitumor and adjunctive intervention in established gastric cancer, alkaloids inhibit proliferation, induce cell-cycle arrest and apoptosis, suppress invasion and metastasis, and regulate non-coding RNA and epigenetic networks; BBR-centered preclinical studies further suggest potential chemosensitizing and supportive effects. This review integrates the five alkaloids BBR, COP, EPI, PAL, and JAT and systematically elucidates their mechanisms of action across the pathological continuum from H. pylori infection and chronic inflammation to precancerous lesions and ultimately gastric cancer. It establishes a stage-oriented, compound-specific analytical framework to clarify the pharmacological positioning of these compounds, identify priorities requiring further validation, and guide future mechanistic and translational research. Full article

Mine leachate and saline industrial wastewater streams are often treated as liabilities to be remediated or disposed of. These flows often contain substantial water and dissolved mineral resources that can be reclaimed. In this work, a waste-heat-assisted falling-film evaporation reactor was developed and assessed for application as a pre-concentration step before water and mineral recovery processes. Two case studies were considered: synthetic saline wastewater containing 80 g/L Na₂SO₄ and 70 g/L NaCl for salt recovery, and iron-rich mine water containing approximately 4000 mg/L Fe²⁺, 95 mg/L Fe³⁺, and 13,000 mg/L acidity as CaCO₃ for downstream pigment and magnetite recovery. Saline water or mine leachate flows down a bank of vertical conduit pipes as a thin film while air flows through the pipe cores. Heat is transferred to the system from industrial waste gas externally. Psychrometric relationships, heat transfer, energy balances, and techno-economic analysis were used to assess the impact of air temperature, conduit diameter, column height, pipe material, and waste-gas temperature on overall reactor performance. Experiments were carried out to confirm expected psychrometric operation and establish appropriate operating temperatures while confirming the impact of conduit geometry on heat-transfer characteristics. A benchmark case of design evaporation rate equal to 100 L/h was chosen for comparison of all tests. Dry air operation was shown to be technically possible but severely limited by the moisture capacity of air; at 26 °C and 101.3 kPa, approximately 205,000 m³/h of air was required. When using industrial waste heat, the operation changed from psychrometric/mass-transfer-limited to heat-transfer-controlled. Using waste gas entering at 144 °C and exiting at 80 °C reduced airflow requirements to approximately 880 m³/h, allowing a much more compact reactor design with approximately 635 (12 mm diameter) conduit pipes. Relative to the 40 °C air benchmark, electrical power was reduced from approximately 24.7 kW to 2.9 kW, and screening-level reactor cost by ~84%. Findings demonstrated that appropriate waste heat enables the application of evaporation if there is sufficient local heat flux. Smaller conduit diameters, sufficient column height, and greater waste-gas inlet temperatures were all beneficial. Choice of material required trade-offs between heat-transfer coefficient, corrosion, and material cost. Full article

Periprosthetic joint infection (PJI) remains one of the most serious complications of arthroplasty, largely due to the formation of microbial biofilms on implant surfaces. Biofilm-associated infections exhibit increased tolerance to antimicrobial therapy and host immune responses, making eradication difficult and often requiring repeated surgical interventions. Consequently, there is a growing need for effective local therapeutic strategies capable of delivering high concentrations of antimicrobial agents directly to the site of infection while minimizing systemic toxicity. Hydrogels have emerged as promising drug delivery platforms for the management of biofilm-associated PJI. Their biocompatibility, injectability, high water content, and tunable physicochemical properties enable controlled and localized release of therapeutic agents within the infected peri-implant environment. This narrative review summarizes recent advances in hydrogel-based approaches, including antibiotic-loaded hydrogels, systems incorporating anti-biofilm enzymes, bacteriophage-loaded formulations, and nanoparticle-enhanced platforms. It also highlights future research directions, with particular emphasis on the need for expanded clinical studies to facilitate the translation of emerging hydrogel-based therapies into clinical practice. Further development of these systems should focus on the incorporation of novel therapeutic agents into hydrogel platforms, aiming to enhance biofilm eradication and improve treatment outcomes in patients with PJI. Particular attention is given to stimuli-responsive (“smart”) hydrogels that release therapeutic payloads in response to infection-related triggers such as pH changes, with emphasis on the need for expanded clinical studies to facilitate the translation of emerging hydrogel-based therapies into clinical practice. Further development of these systems should focus on the incorporation of novel therapeutic agents into hydrogel platforms, aiming to enhance biofilm eradication and improve treatment outcomes in patients with PJI. Full article

Hymenoptera play important ecological roles in agroecosystems as parasitoids, predators, and pollinators. This study investigated the species composition and diversity of Hymenoptera in a conventionally managed pummelo (Citrus maxima (Burm.) Merr.) orchard in Xishuangbanna, Yunnan Province, China, from February 2025 to February 2026. A total of 8446 specimens were collected using Malaise traps and sweep netting, belonging to 11 superfamilies, 37 families, and 191 morphospecies. The community was dominated by parasitoids (140 morphospecies, 73.30%), followed by predators (30 morphospecies, 15.71%), pollinators (18 morphospecies, 9.42%), and phytophagous gall-inducers (3 morphospecies, 1.57%). Ichneumonoidea (42 morphospecies, 21.99%), Chalcidoidea (35 morphospecies, 18.32%), Vespoidea (30 morphospecies, 15.71%) and Apoidea (28 morphospecies, 14.66%) were the dominant superfamilies, collectively accounting for 70.68% of all morphospecies. At the family level, Ichneumonidae (25 morphospecies), Braconidae (17 morphospecies), Bethylidae (13 morphospecies), and Pompilidae and Scelionidae (11 morphospecies each) were the most morphospecies-rich families. Shannon–Wiener diversity was higher at the family level (H′ = 2.9340) than at the superfamily level (H′ = 2.0302), indicating greater heterogeneity at a finer taxonomic resolution. These results demonstrate that the surveyed pummelo orchard in Xishuangbanna harbors a species-rich and functionally diverse hymenopteran community with substantial potential for biological pest control and pollination services. Full article

Lignin-derived chemicals are important renewable building blocks for a sustainable chemical industry, and their selective hydrodeoxygenation (HDO) into cyclohexanol offers a promising route to high-value products; however, efficient C–O bond cleavage over non-noble-metal catalysts remains challenging. Herein, a series of oxygen-vacancy-regulated Co/CeO₂ catalysts was prepared by supporting Co on hydrothermally synthesized CeO₂ nanocubes, with the CeO₂ calcination temperature (400–800 °C) used to tune the defect density and interfacial structure. Low-temperature calcination preserved the nanocubic morphology, high surface area, abundant Ce³⁺–OV sites, and highly dispersed reduced Co species, whereas higher calcination temperatures promoted crystallite growth, surface-area loss, oxygen-vacancy depletion, and Co aggregation. These structural changes directly governed guaiacol HDO performance. Under optimized conditions (160 °C, 2 MPa H₂, 4 h, isopropanol), Co/CeO₂-400 achieved nearly complete guaiacol conversion, with cyclohexanol accounting for approximately 99% of the relative GC–MS product distribution. Mechanistic studies indicate that metallic Co promotes H₂ activation and aromatic-ring hydrogenation, while adjacent Ce³⁺–OV sites facilitate adsorption and cleavage of oxygen-containing groups. The resulting Co–CeO₂ interfacial synergy drives a sequential hydrogenation–deoxygenation pathway and suppresses the accumulation of partially hydrogenated intermediates. Co/CeO₂-400 also showed activity toward representative lignin-derived oxygenates and retained over 90% of its initial activity after five cycles. This work highlights oxygen-vacancy engineering as an effective strategy for designing robust non-noble-metal catalysts for selective lignin valorization. Full article

Betaine aldehyde dehydrogenase (BADH) catalyzes the final step in glycine betaine biosynthesis, but the evolutionary divergence and differential salt responsiveness of BADH homeologs in bread wheat remain unclear. We identified six TaBADH genes and analyzed their phylogenetic relationships, conserved motifs, gene structures, promoter cis-acting elements and synteny. RNA-seq and qRT-PCR were used to compare expression in salt-tolerant Jimai 60 and salt-sensitive Chinese Spring under 200 mM NaCl, and BADH activity, glycine betaine, H₂O₂ and malondialdehyde (MDA) were measured during treatment. The genes separated into chromosome group 2 and group 6 clades with distinct structural and transcriptional patterns. TaBADH-2B encoded a shorter protein and lacked several conserved motifs. Group 6 genes showed stronger salt-responsive expression in Jimai 60, with TaBADH-6D displaying the strongest and most sustained induction. Jimai 60 also showed higher BADH activity and glycine betaine accumulation and lower H₂O₂ and MDA contents at later time points. Expression of TaBADH-6D improved E. coli growth under 200 mM NaCl. These findings identify homeolog-specific divergence within the BADH wheat family and support TaBADH-6D as a candidate for plant-level functional validation. Full article

The Hajdúhát loess plateau in eastern Hungary has no perennial watercourse and overlies a drought-sensitive shallow aquifer, so irrigation supply security and allocation both constrain production. We coupled a dry-2018/wet-2020 MIKE Hydro River model of the East Main Canal (EMC) with a seventeen-year (2009–2025) MIKE SHE groundwater model, and a decision-support layer that couples conveyed-water allocation across sectors with a ranking of management responses. Calibration attains Moriasi Very Good bias-and-balance skill (mean |PBIAS| 1.59%/0.86%), with a head MAE of 1.431 m over 132 wells, and continuous validation reproduces measured discharge to within −7.7% bias over 2022–2025. Growing-season evapotranspiration (448 mm) exceeds precipitation (309 mm), leaving a 314 mm unsaturated-zone deficit; the dry-year canal terminus shows 34 of 92 no-flow days and a sustainability index of 0.09, against 0.34 when wet. A reconciled reach × sector balance and an isolating hydraulic test show the deficit is an allocation problem, not a conveyance limit. A Leopold/analytic-hierarchy-process ranking favors priority-ordered allocation over new capacity, and, in a two-objective time–quantity allocation, re-timing 8.57 × 10${}^6$ m${}^3$ of fish-pond filling into winter would remove the spring deficit without new infrastructure. Dry-year supply is thus a problem of allocation and timing as much as of capacity. Full article

This study investigates the effect of high-temperature hydrogen exposure on the structure and properties of Cr₂O₃/Al₂O₃-based detonation coatings deposited on AISI 316L stainless steel. Bilayer and gradient coatings were exposed to hydrogen at 1000 °C for 3, 4, and 5 h and subsequently characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDS), surface profilometry, and thermal desorption spectroscopy (TDS). One independent specimen was examined for each combination of coating architecture and hydrogen exposure duration. Therefore, the present study was designed as an exploratory comparative investigation rather than a statistically powered study. The principal α-Al₂O₃ and Cr₂O₃ phases remained detectable after all exposure durations, indicating preservation of the main oxide phases. SEM/EDS analysis revealed microcracks, local defects, and heterogeneous surface regions, with more pronounced localized damage in the bilayer coatings. The Ra values of the bilayer coatings were 1.385, 0.833, and 1.207 μm after 3, 4, and 5 h, respectively, whereas the corresponding values for the gradient coatings were 1.049, 1.337, and 1.049 μm. The minimum Ra of 0.833 μm after 4 h in the bilayer coating coincided with SEM/EDS evidence suggesting local coating damage and possible thinning. TDS showed the most intense hydrogen desorption for the gradient coating after 3 h. Overall, the observed results suggest that coating architecture influences surface evolution and hydrogen-retention behavior under the investigated high-temperature hydrogen exposure conditions. Full article

The present study provides the physicochemical and genomic characterization of three Escherichia bacteriophages (6phi8, 6phi10, and 6phi13) isolated from the commercial phage preparation “Sextaphag^®”. For each bacteriophage, lytic activity against E. coli MG1655, as well as pH and thermal stability, were determined. Whole-genome sequencing was performed, followed by bioinformatic annotation and comparative genomic analysis. Bacteriophages 6phi8 and 6phi13 belong to the T4-like myoviruses with large genomes (~169 and ~171 kb, respectively), whereas 6phi10 is a T7-like podovirus with a genome size of 40.1 kb. Phage 6phi8 was assigned to the genus Mosigvirus of the family Straboviridae, 6phi13 was classified within the genus Tequatrovirus of the same family, and 6phi10 was identified as a putative novel species of the genus Berlinvirus within the family Autotranscriptaviridae. The genomes of the studied phages lack genes associated with the lysogenic cycle, as well as virulence and antibiotic resistance determinants. The results expand current knowledge of the genomic properties of phages included in therapeutic cocktails and may contribute to the development of phage preparations against infections caused by E. coli and other members of the family Enterobacteriaceae. Full article