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60 pages, 8707 KiB  
Review
Automation in Construction (2000–2023): Science Mapping and Visualization of Journal Publications
by Mohamed Marzouk, Abdulrahman A. Bin Mahmoud, Khalid S. Al-Gahtani and Kareem Adel
Buildings 2025, 15(15), 2789; https://doi.org/10.3390/buildings15152789 - 7 Aug 2025
Abstract
This paper presents a scientometric review that provides a quantitative perspective on the evolution of Automation in Construction Journal (AICJ) research, emphasizing its developmental paths and emerging trends. The study aims to analyze the journal’s growth and citation impact over time. It also [...] Read more.
This paper presents a scientometric review that provides a quantitative perspective on the evolution of Automation in Construction Journal (AICJ) research, emphasizing its developmental paths and emerging trends. The study aims to analyze the journal’s growth and citation impact over time. It also seeks to identify the most influential publications and the cooperation patterns among key contributors. Furthermore, the study explores the journal’s primary research themes and their evolution. Accordingly, 4084 articles were identified using the Web of Science (WoS) database and subjected to a multistep analysis using VOsviewer version 1.6.18 and Biblioshiny as software tools. First, the growth and citation of the publications over time are inspected and evaluated, in addition to ranking the most influential documents. Second, the co-authorship analysis method is applied to visualize the cooperation patterns between countries, organizations, and authors. Finally, the publications are analyzed using keyword co-occurrence and keyword thematic evolution analyses, revealing five major research clusters: (i) foundational optimization, (ii) deep learning and computer vision, (iii) building information modeling, (iv) 3D printing and robotics, and (v) machine learning. Additionally, the analysis reveals significant growth in publications (54.5%) and citations (78.0%) from 2018 to 2023, indicating the journal’s increasing global influence. This period also highlights the accelerated adoption of digitalization (e.g., BIM, computational design), increased integration of AI and machine learning for automation and predictive analytics, and rapid growth of robotics and 3D printing, driving sustainable and innovative construction practices. The paper’s findings can help readers and researchers gain a thorough understanding of the AICJ’s published work, aid research groups in planning and optimizing their research efforts, and inform editorial boards on the most promising areas in the existing body of knowledge for further investigation and development. Full article
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19 pages, 5733 KiB  
Article
The Production Optimization of a Thermostable Phytase from Bacillus subtilis SP11 Utilizing Mustard Meal as a Substrate
by Md. Al Muid Khan, Sabina Akhter, Tanjil Arif, Md. Mahmuduzzaman Mian, Md. Arafat Al Mamun, Muhammad Manjurul Karim and Shakila Nargis Khan
Fermentation 2025, 11(8), 452; https://doi.org/10.3390/fermentation11080452 - 3 Aug 2025
Viewed by 228
Abstract
Phytate, an antinutritional molecule in poultry feed, can be degraded by applying phytase, but its use in low- and middle-income countries is often limited due to importation instead of local production. Here, inexpensive raw materials were used to optimize the production of a [...] Read more.
Phytate, an antinutritional molecule in poultry feed, can be degraded by applying phytase, but its use in low- and middle-income countries is often limited due to importation instead of local production. Here, inexpensive raw materials were used to optimize the production of a thermostable phytase from an indigenous strain of Bacillus subtilis SP11 that was isolated from a broiler farm in Dhaka. SP11 was identified using 16s rDNA and the fermentation of phytase was optimized using a Plackett–Burman design and response surface methodology, revealing that three substrates, including the raw material mustard meal (2.21% w/v), caused a maximum phytase production of 436 U/L at 37 °C and 120 rpm for 72 h, resulting in a 3.7-fold increase compared to unoptimized media. The crude enzyme showed thermostability up to 80 °C (may withstand the feed pelleting process) with an optimum pH of 6 (near pH of poultry small-intestine), while retaining 96% activity at 41 °C (the body temperature of the chicken). In vitro dephytinization demonstrated its applicability, releasing 978 µg of inorganic phosphate per g of wheat bran per hour. This phytase has the potential to reduce the burden of phytase importation in Bangladesh by making local production and application possible, contributing to sustainable poultry nutrition. Full article
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21 pages, 672 KiB  
Systematic Review
Assessing and Understanding Educators’ Experiences of Synchronous Hybrid Learning in Universities: A Systematic Review
by Hannah Clare Wood, Michael Detyna and Eleanor Jane Dommett
Educ. Sci. 2025, 15(8), 987; https://doi.org/10.3390/educsci15080987 (registering DOI) - 2 Aug 2025
Viewed by 326
Abstract
The rise in online learning, accelerated by the COVID-19 pandemic, has led to greater use of synchronous hybrid learning (SHL) in higher education. SHL allows simultaneous teaching of in-person and online learners through videoconferencing tools. Previous studies have identified various benefits (e.g., flexibility) [...] Read more.
The rise in online learning, accelerated by the COVID-19 pandemic, has led to greater use of synchronous hybrid learning (SHL) in higher education. SHL allows simultaneous teaching of in-person and online learners through videoconferencing tools. Previous studies have identified various benefits (e.g., flexibility) and challenges (e.g., student engagement) to SHL. Whilst systematic reviews have emerged on this topic, few studies have considered the experiences of staff. The aim of this review was threefold: (i) to better understand how staff experiences and perceptions are assessed, (ii) to understand staff experiences in terms of the benefits and challenges of SHL and (iii) to identify recommendations for effective teaching and learning using SHL. In line with the PRISMA guidance, we conducted a systematic review across four databases, identifying 14 studies for inclusion. Studies were conducted in nine different countries and covered a range of academic disciplines. Most studies adopted qualitative methods, with small sample sizes. Measures used were typically novel and unvalidated. Four themes were identified relating to (i) technology, (ii) redesigning teaching and learning, (iii) student engagement and (iv) staff workload. In terms of recommendations, ensuring adequate staff training and ongoing classroom support were considered essential. Additionally, active and collaborative learning were considered important to address issues with interactivity. Whilst these findings largely aligned with previous work, this review also identified limited reporting in research in this area, and future studies are needed to address this. Full article
(This article belongs to the Section Higher Education)
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19 pages, 18533 KiB  
Article
Modeling of Marine Assembly Logistics for an Offshore Floating Photovoltaic Plant Subject to Weather Dependencies
by Lu-Jan Huang, Simone Mancini and Minne de Jong
J. Mar. Sci. Eng. 2025, 13(8), 1493; https://doi.org/10.3390/jmse13081493 - 2 Aug 2025
Viewed by 133
Abstract
Floating solar technology has gained significant attention as part of the global expansion of renewable energy due to its potential for installation in underutilized water bodies. Several countries, including the Netherlands, have initiated efforts to extend this technology from inland freshwater applications to [...] Read more.
Floating solar technology has gained significant attention as part of the global expansion of renewable energy due to its potential for installation in underutilized water bodies. Several countries, including the Netherlands, have initiated efforts to extend this technology from inland freshwater applications to open offshore environments, particularly within offshore wind farm areas. This development is motivated by the synergistic benefits of increasing site energy density and leveraging the existing offshore grid infrastructure. The deployment of offshore floating photovoltaic (OFPV) systems involves assembling multiple modular units in a marine environment, introducing operational risks that may give rise to safety concerns. To mitigate these risks, weather windows must be considered prior to the task execution to ensure continuity between weather-sensitive activities, which can also lead to additional time delays and increased costs. Consequently, optimizing marine logistics becomes crucial to achieving the cost reductions necessary for making OFPV technology economically viable. This study employs a simulation-based approach to estimate the installation duration of a 5 MWp OFPV plant at a Dutch offshore wind farm site, started in different months and under three distinct risk management scenarios. Based on 20 years of hindcast wave data, the results reveal the impacts of campaign start months and risk management policies on installation duration. Across all the scenarios, the installation duration during the autumn and winter period is 160% longer than the one in the spring and summer period. The average installation durations, based on results from 12 campaign start months, are 70, 80, and 130 days for the three risk management policies analyzed. The result variation highlights the additional time required to mitigate operational risks arising from potential discontinuity between highly interdependent tasks (e.g., offshore platform assembly and mooring). Additionally, it is found that the weather-induced delays are mainly associated with the campaigns of pre-laying anchors and platform and mooring line installation compared with the other campaigns. In conclusion, this study presents a logistics modeling methodology for OFPV systems, demonstrated through a representative case study based on a state-of-the-art truss-type design. The primary contribution lies in providing a framework to quantify the performance of OFPV installation strategies at an early design stage. The findings of this case study further highlight that marine installation logistics are highly sensitive to local marine conditions and the chosen installation strategy, and should be integrated early in the OFPV design process to help reduce the levelized cost of electricity. Full article
(This article belongs to the Special Issue Design, Modeling, and Development of Marine Renewable Energy Devices)
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18 pages, 4051 KiB  
Article
Chimeric Vesicular Stomatitis Virus Bearing Western Equine Encephalitis Virus Envelope Proteins E2-E1 Is a Suitable Surrogate for Western Equine Encephalitis Virus in a Plaque Reduction Neutralization Test
by Kerri L. Miazgowicz, Bailey E. Maloney, Melinda A. Brindley, Mattie Cassaday, Raegan J. Petch, Paul Bates, Aaron C. Brault and Amanda E. Calvert
Viruses 2025, 17(8), 1067; https://doi.org/10.3390/v17081067 - 31 Jul 2025
Viewed by 290
Abstract
In December 2023, infections of western equine encephalitis virus (WEEV) within Argentina were reported to the World Health Organization (WHO). By April 2024, more than 250 human infections, 12 of which were fatal, and 2500 equine infections were identified in South America. Laboratory [...] Read more.
In December 2023, infections of western equine encephalitis virus (WEEV) within Argentina were reported to the World Health Organization (WHO). By April 2024, more than 250 human infections, 12 of which were fatal, and 2500 equine infections were identified in South America. Laboratory diagnosis and surveillance in affected countries were hindered by a lack of facilities equipped with BSL-3 laboratories, as confirmatory serodiagnosis for WEEV requires live virus in the plaque reduction neutralization test (PRNT). To expand serodiagnosis for WEEV in the Americas, we developed a virus chimera composed of vesicular stomatitis virus (VSV) engineered to display the E2-E1 glycoproteins of WEEV (VSV/WEEV) in place of the VSV glycoprotein (G). PRNT90 and IC90 values of parental WEEV and VSV/WEEV were analogous using sera collected from mice, horses, and chickens. VSV/WEEV rapidly formed plaques with clear borders and reduced the assay readout time by approximately 8 h compared to the parental virus. Overall, we demonstrate that chimeric VSV/WEEV is a suitable surrogate for WEEV in a diagnostic PRNT. Use of chimeric VSV/WEEV in place of authentic WEEV will dramatically expand testing capacity by enabling PRNTs to be performed at BSL-2 containment, while simultaneously decreasing the health risk to testing personnel. Full article
(This article belongs to the Special Issue Mosquito-Borne Encephalitis Viruses)
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12 pages, 7989 KiB  
Article
Microstructures and Magnetic Properties of Rare-Earth-Free Co-Zr-Mo-B Alloys
by Tetsuji Saito and Masaru Itakura
Crystals 2025, 15(8), 698; https://doi.org/10.3390/cryst15080698 - 31 Jul 2025
Viewed by 263
Abstract
The growing demand for rare-earth magnets has raised concerns over their price and the country’s risk of depleting the supply of rare-earth elements. These severe concerns have led to the study of rare-earth-free magnets that do not rely on rare-earth elements. Co-Zr-Mo-B alloys, [...] Read more.
The growing demand for rare-earth magnets has raised concerns over their price and the country’s risk of depleting the supply of rare-earth elements. These severe concerns have led to the study of rare-earth-free magnets that do not rely on rare-earth elements. Co-Zr-Mo-B alloys, one of the prospective candidates for rare-earth-free magnets, were produced by the melt-spinning technique and subsequent annealing. It was found that a small substitution of Mo for Zr in the Co-Zr-B alloys increased coercivity. The Co-Zr-Mo-B alloy with a Mo content of 2 at% showed a high coercivity of 6.2 kOe with a remanence of 40 emu/g. SEM studies showed that the annealed Co-Zr-Mo-B alloys had fine, uniform grains with an average diameter of about 0.6 μm. Further studies using STEM demonstrated that the ferromagnetic phase in the annealed Co-Zr-Mo-B alloys with high coercivity was composed of the Co5Zr phase and the long-period stacking ordered (LPSO) phase. That is, the fine grains observed in the SEM studies were found to be ferromagnetic dendrites containing numerous twin boundaries of the Co5Zr phase and its derived LPSO phase. Therefore, the high coercivity of the Co-Zr-Mo-B alloys can be attributed to the presence of ferromagnetic crystals of Co5Zr and the derived LPSO phase. Full article
(This article belongs to the Special Issue Innovations in Magnetic Composites: Synthesis to Application)
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22 pages, 1272 KiB  
Review
Pharmacy Technicians in Immunization Services: Mapping Roles and Responsibilities Through a Scoping Review
by Carolina Valeiro, Vítor Silva, Jorge Balteiro, Diane Patterson, Gilberto Bezerra, Karen Mealiff, Cristiano Matos, Ângelo Jesus and João Joaquim
Healthcare 2025, 13(15), 1862; https://doi.org/10.3390/healthcare13151862 - 30 Jul 2025
Viewed by 209
Abstract
Background: Pharmacy technicians are increasingly involved in immunization services, enhancing vaccine accessibility and reducing pharmacies’ workload. This scoping review aims to (1) provide a comprehensive overview of pharmacy technicians’ involvement in immunization services across various healthcare settings and countries, and (2) conduct a [...] Read more.
Background: Pharmacy technicians are increasingly involved in immunization services, enhancing vaccine accessibility and reducing pharmacies’ workload. This scoping review aims to (1) provide a comprehensive overview of pharmacy technicians’ involvement in immunization services across various healthcare settings and countries, and (2) conduct a comparative analysis of training curricula for pharmacy technicians on immunization. Methods: A scoping review was conducted following the Arksey and O’Malley framework. A comprehensive search of the PubMed and Scopus databases was performed using keywords and MeSH terms such as “pharmacy technician(s)”, “immunization”, “vaccination”, “role”, and “involvement”. Studies included assessed pharmacy technicians’ roles in vaccine administration, training, and public health outcomes. Descriptive and thematic analyses were used to synthesize the findings. In addition, a supplementary analysis of immunization training curricula was conducted, reviewing programs from different countries to identify similarities, differences, and gaps in course structure, content, and delivery formats. Lastly, a comprehensive toolkit was developed, offering guidelines intended to facilitate the implementation of immunization training programs. Results: A total of 35 articles met the inclusion criteria, primarily from the United States of America (n = 30), Canada (n = 2), Ethiopia (n = 1), Denmark (n = 1) and United Kingdom (n = 1). The findings indicate that pharmacy technicians contribute significantly to vaccine administration, patient education, and workflow optimization, particularly in community pharmacies. The COVID-19 pandemic accelerated their involvement in immunization programs. Key challenges include regulatory barriers, a lack of standardized training, and resistance from other healthcare professionals. Facilitators include legislative support (e.g., the PREP Act), structured training programs, and collaborative pharmacist–technician models. Conclusions: Pharmacy technicians can play a vital role in expanding immunization services, improving vaccine uptake, and reducing pharmacist workload. Addressing regulatory inconsistencies, enhancing training, and fostering interprofessional collaboration are crucial for their effective integration of immunization programs. Since immunization by pharmacy technicians is not yet allowed in many EU countries, this review will provide a foundational basis to address their potential to support the healthcare workforce and improve access to immunization services. Full article
(This article belongs to the Special Issue Policy Interventions to Promote Health and Prevent Disease)
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12 pages, 500 KiB  
Review
Beyond the Pill: Mapping Process-Oriented Decision Support Models in Pharmaceutical Policy
by Foteini Theiakou, Catherine Kastanioti, Dimitris Zavras and Dimitrios Rekkas
Healthcare 2025, 13(15), 1861; https://doi.org/10.3390/healthcare13151861 - 30 Jul 2025
Viewed by 242
Abstract
Background: The quality of decision-making processes is increasingly recognized as critical to public trust and policy sustainability. Objectives: This narrative review aims to identify and describe process-focused decision support models (DSMs) applied in pharmaceutical policy, and to examine their potential contributions [...] Read more.
Background: The quality of decision-making processes is increasingly recognized as critical to public trust and policy sustainability. Objectives: This narrative review aims to identify and describe process-focused decision support models (DSMs) applied in pharmaceutical policy, and to examine their potential contributions to improving procedural quality in decisions related to pricing, reimbursement, and access to medicines. Methods: Relevant peer-reviewed and gray literature published between 2000 and 2025 was considered, drawing from key databases (e.g., PubMed and Scopus) and international policy reports (e.g., WHO, ISPOR, and HTA agencies). Studies were included if they provided insights into DSMs addressing at least one dimension of decision process quality. Results: Findings are synthesized narratively and organized by tool type, application context, and key quality dimensions. Frequently referenced tools included the Quality of Decision-Making Orientation Scheme (QoDoS), WHO-INTEGRATE, and AGREE II. QoDoS emerged as the only tool applied across regulatory, HTA, and industry settings, evaluating both individual- and organizational-level practices. WHO-INTEGRATE highlighted equity and legitimacy considerations but lacked a structured format. Overall, most tools demonstrated benefits in promoting internal consistency, transparency, and stakeholder engagement; however, their adoption remains limited, especially in low- and middle-income countries. Conclusions: Process-focused DSMs offer promising avenues for enhancing transparency, consistency, and legitimacy in pharmaceutical policy. Further exploration is needed to standardize evaluation approaches and expand the use of DSMs in diverse health systems. Full article
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25 pages, 516 KiB  
Article
Exploring a Sustainable Pathway Towards Enhancing National Innovation Capacity from an Empirical Analysis
by Sylvia Novillo-Villegas, Ana Belén Tulcanaza-Prieto, Alexander X. Chantera and Christian Chimbo
Sustainability 2025, 17(15), 6922; https://doi.org/10.3390/su17156922 - 30 Jul 2025
Viewed by 239
Abstract
Innovation is a strategic driver of sustainable competitive advantage and long-term economic growth. This study proposes an empirical framework to support the sustained development of national innovation capacity by examining key enabling factors. Drawing on an extensive review of the literature, the research [...] Read more.
Innovation is a strategic driver of sustainable competitive advantage and long-term economic growth. This study proposes an empirical framework to support the sustained development of national innovation capacity by examining key enabling factors. Drawing on an extensive review of the literature, the research investigates the interrelationships among governmental support (GS), innovation agents (IA), university–industry R&D collaborations (UIRD), and innovation cluster development (ICD), and their influence on two critical innovation outcomes, knowledge creation (KC) and knowledge diffusion (KD). Using panel data from G7 countries spanning 2008 to 2018, sourced from international organizations such as the World Bank, the World Intellectual Property Organization, and the World Economic Forum, the study applies regression analysis to test the proposed conceptual model. Results highlight the foundational role of GS in providing a balanced framework to foster collaborative networks among IA and enhancing the effectiveness of UIRD. Furthermore, IA emerges as a pivotal actor in advancing innovation efforts, while the development of innovation clusters is shown to selectively enhance specific innovation outcomes. These findings offer theoretical and practical contributions for policymakers, researchers, and stakeholders aiming to design supportive ecosystems that strengthen sustainable national innovation capacity. Full article
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24 pages, 2315 KiB  
Article
A Decade of Transformation in Higher Education and Science in Kazakhstan: A Literature and Scientometric Review of National Projects and Research Trends
by Timur Narbaev, Diana Amirbekova and Aknar Bakdaulet
Publications 2025, 13(3), 35; https://doi.org/10.3390/publications13030035 - 30 Jul 2025
Viewed by 379
Abstract
Higher education and science (HES) is one of the key drivers of a country’s economic growth. In this study, we examine national projects and research capacity in HES in Kazakhstan from 2014 to 2024. We conducted a content review and scientometric analysis with [...] Read more.
Higher education and science (HES) is one of the key drivers of a country’s economic growth. In this study, we examine national projects and research capacity in HES in Kazakhstan from 2014 to 2024. We conducted a content review and scientometric analysis with network and temporal visualizations. Our data sources included policy documents, statistical reports, and the Scopus database. Our findings suggest that, while Kazakhstan aligns with global trends in the field (e.g., digitalization, scientometrics monitoring, and internationalization), these are achieved through a state-led, policy-driven approach shaped by its post-Soviet context. Additionally, we note a dual structure in Kazakhstan’s HES sector, characterized by a strong top-down direction and increasing institutional engagement. In terms of the thematic trends from the temporal analysis, the country experienced a three-staged evolution: foundational reforms and system modernization (2014–2017), capacity building and evaluation (2018–2021), and, most recently, strategic expansion, inclusivity, and globalization (2022–2024). Throughout the analyzed period, low R&D intensity, disciplinary imbalances, and structural barriers still undermine desired development efforts in HES. The analyzed case of Kazakhstan can serve as “lessons learned” for policymakers and researchers working in the science evaluation and scholarly communication area in similar emerging or transition countries. Full article
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26 pages, 632 KiB  
Article
When Do Innovation and Renewable Energy Transition Drive Environmental Sustainability?
by Anis Omri, Fadhila Hamza and Noura Alkahtani
Sustainability 2025, 17(15), 6910; https://doi.org/10.3390/su17156910 - 30 Jul 2025
Viewed by 279
Abstract
This study examines the contributions of renewable energy transition (RET) and environmental innovation (EI) to environmental performance in G7 countries from 2003 to 2021, with a focus on the transmission channels of green finance and environmental governance. Using the Augmented Mean Group (AMG) [...] Read more.
This study examines the contributions of renewable energy transition (RET) and environmental innovation (EI) to environmental performance in G7 countries from 2003 to 2021, with a focus on the transmission channels of green finance and environmental governance. Using the Augmented Mean Group (AMG) estimator and confirming robustness through the Dynamic Common Correlated Effects Mean Group (DCCE-MG) method, the study explores both direct and indirect effects of RET and EI on two key environmental indicators: the Environmental Performance Index and the Load Capacity Factor. The results reveal that both RET and EI have a significant impact on environmental performance. Moreover, green finance and environmental governance serve as crucial channels through which RET and EI exert their influence. These findings underscore the importance of developing effective financial instruments and robust regulatory frameworks to translate energy and innovation policies into tangible environmental benefits. By highlighting the interplay between technological advancement, financial capacity, and institutional quality, this study provides novel insights into the environmental policy landscape of advanced economies and offers guidance for designing integrated strategies to achieve long-term sustainability goals. Full article
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18 pages, 670 KiB  
Article
Genetic Heterogeneity Correlated with Phenotypic Variability in 48 Patients with Cystic Fibrosis
by Mădălina Andreea Donos, Lăcrămioara Ionela Butnariu, Dana Teodora Anton Păduraru, Alina Mariela Murgu, Cristina Rusu, Monica Cristina Pânzaru, Roxana Popescu, Elena Țarcă, Elena Cojocaru, Gabriela Ghiga and Laura Mihaela Trandafir
J. Clin. Med. 2025, 14(15), 5362; https://doi.org/10.3390/jcm14155362 - 29 Jul 2025
Viewed by 230
Abstract
Background/Objectives: Cystic fibrosis (CF) is a rare autosomal recessive genetic disease that has a progressive and multisystemic course. The spectrum and frequency of mutations in the gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR) vary both in European countries and in [...] Read more.
Background/Objectives: Cystic fibrosis (CF) is a rare autosomal recessive genetic disease that has a progressive and multisystemic course. The spectrum and frequency of mutations in the gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR) vary both in European countries and in other geographical regions. The aim of our retrospective study was to present the genetic variants identified in a group of 48 CF patients from the Moldova region (Romania), as well as to establish genotype–phenotype correlations. Methods: Genetic testing was initially performed for 38 CFTR mutations, and in heterozygous patients or those in whom no mutation was detected, CFTR gene sequencing (NGS) was performed. Results: The compound heterozygous genotype was identified in 26 (54.16%) of the patients (with one of the alleles being F508del), while 22 (45.83%) patients had the homozygous F508del genotype. The F508del variant was the most frequent (69.79%), followed by G542X (6.25%, 6/96). Several new variants were also identified that had not been reported in other studies from Romania (R1158X, K598*, R347H, c.2589_2599del, R496H, and CFTRdele2). Phenotypic manifestations in patients with CFTR class I, II, III and VII variants (homozygous and compound heterozygous) were more severe compared to those in patients with CFTR class IV, V and VI mutations, with the data obtained being consistent with those in the literature. Respiratory tract involvement was present in 77.08% of the patients, being more frequent in patients with the compound heterozygous genotype compared to the homozygous F508del genotype. Most patients had exocrine pancreatic insufficiency (EPI) (85.41%). Gastrointestinal manifestations included hepatocytolysis (66.66%) and biliary cirrhosis (0.41%). Meconium ileus was detected in 18.75% of patients, all with a compound heterozygous genotype. Conclusions: We compared the results obtained with data from the literature and correlated the detected CFTR variant (genotype) with the phenotypic manifestations, highlighting certain particularities present in some patients. Genetic testing allows for early diagnosis and adapted management, including personalized treatment for each patient. Identification of novel unclassified CFTR variants still remains a challenge for clinicians. NGS-based screening of heterozygous healthy carriers is important for both genetic counseling and prenatal diagnosis. Full article
(This article belongs to the Special Issue Cystic Fibrosis: Clinical Manifestations and Treatment)
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18 pages, 4319 KiB  
Article
Exploring the Synthesis of Lactic Acid from Sugarcane Molasses Collected in Côte d’Ivoire Using Limosilactobacillus fermentum ATCC 9338 in a Batch Fermentation Process
by Asengo Gerardin Mabia, Harinaivo Anderson Andrianisa, Chiara Danielli, Leygnima Yaya Ouattara, N’da Einstein Kouadio, Esaïe Kouadio Appiah Kouassi, Lucia Gardossi and Kouassi Benjamin Yao
Bioengineering 2025, 12(8), 817; https://doi.org/10.3390/bioengineering12080817 - 29 Jul 2025
Viewed by 244
Abstract
Lactic acid (LA) is a high-value chemical with growing demand for the production of polymers and plastics and in the food and pharmaceutical industries. However, production costs remain a significant constraint when using conventional food-grade substrates. This study investigates Ivorian sugarcane molasses, an [...] Read more.
Lactic acid (LA) is a high-value chemical with growing demand for the production of polymers and plastics and in the food and pharmaceutical industries. However, production costs remain a significant constraint when using conventional food-grade substrates. This study investigates Ivorian sugarcane molasses, an abundant agro-industrial by-product, as a low-cost carbon source for LA production via batch fermentation with Limosilactobacillus fermentum ATCC 9338. Molasses was pretreated by acid hydrolysis to improve fermentability, increasing glucose and fructose concentrations. Comparative fermentations using raw and pretreated molasses showed a 75% increase in LA production (32.4 ± 0.03 g/L) after pretreatment. Optimisation using Box–Behnken design revealed that the initial sugar concentration, inoculation rate, and stirring speed significantly influenced lactic acid production. Under optimal conditions, a maximum LA concentration of 52.4 ± 0.49 g/L was achieved with a yield of 0.95 g/g and productivity of 0.73 g/L·h. Kinetic analysis confirmed efficient sugar utilisation under the optimised conditions, and polarimetry revealed a near-racemic lactic acid. A simplified cost analysis showed that molasses could reduce carbon source costs by over 70% compared to refined sugars, supporting its economic viability. This work demonstrates the potential of pretreated molasses under robust fermentation conditions as a sustainable and cost-effective substrate for LA production in resource-limited contexts. The approach aligns with circular bioeconomy principles and presents a replicable model for decentralised bioproduction in a developing country like Côte d’Ivoire. Full article
(This article belongs to the Special Issue Development of Biocatalytic Processes and Green Energy Technologies)
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18 pages, 2105 KiB  
Communication
Morphological and Nutritional Characterization of the Native Sunflower as a Potential Plant Resource for the Sierra Gorda of Querétaro
by Ana Patricia Arenas-Salazar, Mark Schoor, María Isabel Nieto-Ramírez, Juan Fernando García-Trejo, Irineo Torres-Pacheco, Ramon Gerardo Guevara-González, Humberto Aguirre-Becerra and Ana Angélica Feregrino-Pérez
Resources 2025, 14(8), 121; https://doi.org/10.3390/resources14080121 - 29 Jul 2025
Viewed by 413
Abstract
Problems with primary food production (food insecurity, malnutrition, and socioeconomic problems) persist throughout the world, especially in rural areas. Despite these problems, the available natural food resources are underutilized; residents are no longer interested in growing and consuming foods native to their region. [...] Read more.
Problems with primary food production (food insecurity, malnutrition, and socioeconomic problems) persist throughout the world, especially in rural areas. Despite these problems, the available natural food resources are underutilized; residents are no longer interested in growing and consuming foods native to their region. In this regard, this study carries out the morphological and nutritional characterization of a native sunflower (Helianthus annuus) grown in the Sierra Gorda, Querétaro, Mexico, known as “Maíz de teja”, to implement a sustainable monoculture production system. The results were compared with some other sunflower varieties and other oilseeds grown and consumed in the country. This study determined that this native sunflower seed is a good source of linoleic acid (84.98%) and zinc (17.2 mg/100 g). It is an alternative protein source (18.6 g/100 g), comparable to foods of animal origin. It also provides a good amount of fiber (22.6 g/100 g) and bioactive compounds (total phenolic compounds (TPC) 3.434 ± 0.03 mg/g and total flavonoids (TFC) 0.67 ± 0.02 mg/g), and seed yield 341.13 kg/ha. This study demonstrated a valuable nutritional profile of this native seed and its potential for cultivation. Further research is needed to improve agricultural management to contribute to food security and improve the socioeconomic status of the community. Full article
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23 pages, 1019 KiB  
Article
Deciphering the Environmental Consequences of Competition-Induced Cost Rationalization Strategies of the High-Tech Industry: A Synergistic Combination of Advanced Machine Learning and Method of Moments Quantile Regression Procedures
by Salih Çağrı İlkay, Harun Kınacı and Esra Betül Kınacı
Sustainability 2025, 17(15), 6867; https://doi.org/10.3390/su17156867 - 28 Jul 2025
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Abstract
This study intends to portray how varying degrees of environmental policy stringency and the growing pressure of global competition reflect on high-tech (HT) sectors’ cost rationalization strategies and lead to environmental consequences in 15 G20 countries (1992–2019). Moreover, we center the pattern of [...] Read more.
This study intends to portray how varying degrees of environmental policy stringency and the growing pressure of global competition reflect on high-tech (HT) sectors’ cost rationalization strategies and lead to environmental consequences in 15 G20 countries (1992–2019). Moreover, we center the pattern of cost rationalization management regarding the opportunity cost of ecosystem service consumption and propose to test the fundamental hypothesis stating the possible transmission of competition-induced technological innovations to green economic transformation. Our new methodology estimates quantile-specific effects with MM-QR, while identifying the main interaction effects between regulatory pressure and trade competition uses an extended STIRPAT model. The results reveal a paradoxical finding: despite higher environmental policy stringency and opportunity costs of ecosystem services, HT sectors persistently adopt environmentally detrimental cost-reduction approaches. These findings carry important policy implications: (1) environmental regulations for HT sectors require complementary innovation subsidies, (2) trade agreements should incorporate clean technology transfer clauses, and (3) governments must monitor sectoral emission leakage risks. Our dual machine learning–econometric approach provides policymakers with targeted insights for different emission scenarios, highlighting the need for differentiated strategies across clean and polluting HT subsectors. Full article
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