Search Results (25)

Background/Objectives: Large-scale, country-specific data on skin cancer predictors are scarce in Hungary. The Euromelanoma campaign offers a decade-long opportunity to investigate constitutional, behavioral, and motivational risk factors in a Central European setting through a national cross-sectional analysis. Methods: Between 2009 and 2018, Hungarian participants underwent dermatological screening. Diagnoses of clinically suspicious skin cancers were based on dermoscopic assessment, as histopathological confirmation was not systematically available. Among 18,598 standardized surveys, logistic regression identified independent predictors of clinically suspicious skin cancers overall and separately for melanoma and non-melanoma skin cancers (NMSCs). Results: Clinically suspicious skin cancers were detected in 3.9% of participants (1.7% melanoma, 2.3% NMSC). Strong predictors across all cancer types were atypical nevi (OR 4.75), personal history of NMSC (OR 3.42), and melanoma (OR 1.99). For melanoma, atypical nevi (OR 13.12), prior melanoma (OR 5.95), heavy sunbed use (OR 2.15), and trunk lentigines (OR 1.47) were significant. For NMSC, age (OR 1.08 per year), personal history of NMSC (OR 4.75), family history of melanoma (OR 2.41), and atypical nevi (OR 1.76) were dominant. Screening motivation influenced detection: participants attending for a changing lesion had higher odds of suspicious findings, whereas those attending for routine checks, family/friend history, or “many moles” had lower odds. Conclusions: Over a decade of Euromelanoma screening, atypical nevi, prior skin cancer history, and heavy sunbed use emerged as the strongest predictors of suspicious skin cancers. Participant motivation shaped detection patterns, supporting risk-stratified screening, targeted public education, and stricter regulation of artificial ultraviolet exposure. Full article

Acetate is naturally produced in the rumen through feed degradation and fermentation. It serves as a primary energy source for ruminants and as a key substrate for de novo fatty acid synthesis in the mammary gland. The interaction of exogenous acetate with different animal and dietary factors is an area of growing interest, as it may have significant implications for milk fat synthesis. This study aimed to assess the effect of two diet fermentability levels on the short-term response of lactation to acetate supplementation in dairy cows. Eight ruminally cannulated multiparous European Holstein cows were randomly assigned to treatments in a crossover design that tested the effect of diet fermentability, acetate supply, and their interaction. Using corn silage as the only forage source and a constant forage-to-concentrate ratio, high-fermentability (HF) and low-fermentability (LF) diets were formulated. Acetate supply was investigated by infusing ruminally 10 moles of sodium acetate/d (ACE) or an equimolar infusion of control (CON). Therefore, the treatments were as follows: LF + CON; LF + ACE; HF + CON; and HF + ACE. No interactions between acetate and diet fermentability were found on performance variables. Acetate infusion decreased dry matter intake (DMI), milk yield, and milk protein yield and content but did not affect milk fat yield; however, it increased milk fat concentration, and this response tended to be more pronounced in the HF diet. Acetate infusions increased plasma β-hydroxybutyrate in the HF diet, but not in the LF diet, and increased plasma non-esterified fatty acid, which was likely a lipolysis response to reduced DMI and decreased energy balance. This study demonstrates that acetate availability can be a constraint on mammary lipogenesis, even with adequate dietary fiber. Full article

The COllaborative Carbon Column Observing Network has become a reliable source of high-quality ground-based remote sensing network data that provide column-averaged dry-air mole fractions of carbon dioxide (XCO₂), methane (XCH₄), and carbon monoxide (XCO). The fiducial reference measurements of these gases from the COCCON complement the TCCON and NDACC-IRWG data. This study shows the application of COCCON data for the validation of existing greenhouse gas satellite products. This study includes the validation of XCH₄ and XCO products from the European Copernicus Sentinel-5 Precursor (S5P) mission, XCO₂ products from the American Orbiting Carbon Observatory-2 (OCO-2) mission, and XCO₂ and XCH₄ products from the Japanese Greenhouse gases Observing SATellite (GOSAT). A total of 27 datasets contributed to this study; some of these were collected in the framework of campaign activities and covered only a short time period. In addition, several permanent stations provided long-term observations. The random uncertainties in the validation results, specifically for S5P with a lot of coincidences pairs, are found to be similar to the comparison with the TCCON. The comparison results of OCO-2 land nadir and land glint observation modes to the COCCON on a global scale, despite limited coincidences, are very promising. The stations can, therefore, expand on the coverage of the already existing ground-based reference remote sensing sites from the TCCON and the NDACC network. The COCCON data can be used for future satellite and model validation studies and carbon cycle studies. Full article

A good quantitative knowledge of regional sources and sinks of atmospheric carbon dioxide (CO₂) is essential for understanding the global carbon cycle. It is also a key prerequisite for elaborating cost-effective national strategies to achieve the goals of the Paris Agreement. However, available estimates of CO₂ fluxes for many regions of the world remain uncertain, despite significant recent progress in the remote sensing of terrestrial vegetation and atmospheric CO₂. In this study, we investigate the feasibility of inferring reliable regional estimates of the net ecosystem exchange (NEE) using column-averaged dry-air mole fractions of CO₂ (XCO₂) retrieved from Orbiting Carbon Observatory-2 (OCO-2) observations as constraints on parameters of the widely used Vegetation Photosynthesis and Respiration model (VPRM), which predicts ecosystem fluxes based on vegetation indices derived from multispectral satellite imagery. We developed a regional-scale inverse modeling system that applies a Bayesian variational optimization algorithm to optimize parameters of VPRM coupled to the CHIMERE chemistry transport model and which involves a preliminary transformation of the input XCO₂ data that reduces the impact of the CHIMERE boundary conditions on inversion results. We investigated the potential of our inversion system by applying it to a European region (that includes, in particular, the EU countries and the UK) for the warm season (May–September) of 2021. The inversion of the OCO-2 observations resulted in a major (more than threefold) reduction of the prior uncertainty in the regional NEE estimate. The posterior NEE estimate agrees with independent estimates provided by the CarbonTracker Europe High-Resolution (CTE-HR) system and the ensemble of the v10 OCO-2 model intercomparison (MIP) global inversions. We also found that the inversion improves the agreement of our simulations of XCO₂ with retrievals from the Total Carbon Column Observing Network (TCCON). Our sensitivity test experiments using synthetic XCO₂ data indicate that the posterior NEE estimate would remain reliable even if the actual regional CO₂ fluxes drastically differed from their prior values. Furthermore, the posterior NEE estimate is found to be robust to strong biases and random uncertainties in the CHIMERE boundary conditions. Overall, this study suggests that our approach offers a reliable and relatively simple way to derive robust estimates of CO₂ ecosystem fluxes from satellite XCO₂ observations while enhancing the applicability of VPRM in regions where eddy covariance measurements of CO₂ fluxes are scarce. Full article

Here, for the first time, the structure of genes involved in sex determination in mammals (full Sry and partial Rspo1, Eif2s3x, and Eif2s3y) was analyzed for the European mole Talpa europaea with ovotestes in females. We confirmed male-specificity for Eif2s3y and Sry. Five exons were revealed for Rspo1 and the deep similarity with the structure of this gene in T. occidentalis was proved. The most intriguing result was obtained for the Sry gene, which, in placental mammals, initiates male development. We described two exons for this canonically single-exon gene: the first (initial) exon is only 15 bp while the second exon includes 450 bp. The exons are divided by an extended intron of about 1894 bp, including the fragment of the LINE retroposon. Moreover, in chromatogram fragments, which correspond to intron and DNA areas, flanking both exons, we revealed double peaks, similar to heterozygous nucleotide sites of autosomal genes. This may indicate the existence of two or more copies of the Sry gene. Proof of copies requires an additional in-depth study. We hypothesize that unusual structure and possible supernumerary copies of Sry may be involved in ovotestes formation. Full article

Insectivores (Eulypotiphla) are a substantial component of Russian forest ecosystems. The parasites of these animals also form an important part of natural biocenoses and act as one of the factors in the formation of biodiversity. The Mordovia Nature Reserve and National Park “Smolny” are large, forested areas located in the center of European Russia. We studied the helminth fauna of insectivores in these protected areas in 2018–2022. In total, using the method of complete helminthological necropsy, we examined 478 individuals of shrews, moles, and hedgehogs and recorded 34 species of parasitic worms, i.e., 8 trematode, 7 cestode, 1 acanthocephalan, and 18 nematode species. The most diverse helminth fauna was found in Sorex araneus (22 species). The composition of helminths in S. isodon (12), Neomys fodiens (9), Sorex minutus, and Erinaceus roumanicus (8 species each) turned out to be less diverse. The lowest species diversity of helminths was observed in Neomys milleri (3) and Talpae europaea (2 species). Taking into account the newly obtained data, we conducted a review of the helminth diversity in shrews, hedgehogs, and moles in the Middle Volga region. According to our literature data, the helminth fauna of insectivores in this region consists of 52 species, including 14 cestodes, 13 trematodes, 22 nematodes, and 3 acanthocephalans. Most of them belong to the Palearctic faunal complex (36 species). The helminth fauna of insectivores in the studied protected areas was compared with the helminth fauna of micromammals in other areas of the Middle Volga region. Our comparative analysis showed a high and average degree of similarity in the helminth fauna within individual species and genera of Eulipotyphla. Full article

Blind mole rats (genus Nannospalax) attract a great deal of attention because of their cancer resistance and longevity. Due to the high rate of chromosome rearrangements, 74 Nannospalax chromosomal forms have been discovered. The convergence of their external morphology complicates their taxonomy, and many cryptic species remain unrecognized. Thus, the European N. leucodon supersp. is listed in the IUCN Red List of Threatened Species with “Data Deficient” status. It is crucial for the conservation of biodiversity to clarify its taxonomy, to recognize each cryptic species, and assign to them the correct conservation status. Of the more than 20 chromosomal forms described within N. leucodon, five cryptic species occur in Serbia. The most threatened among them—N. l. syrmiensis, described and named 50 years ago in the regions of Srem, Belgrade and Mačva—has been declared extinct in the literature, which may have negative consequences for the conservation of wildlife genetic diversity. Through five years of fieldwork and comparison of 16SrRNA and MT-CYTB gene segments between old, archived teeth and recently collected material, we show that N. l. syrmiensis is not extinct. However, its habitat has been fragmented and reduced, owing primarily to anthropogenic impact. Therefore, detailed surveillance, population-structure studies, risk assessment, and appropriate conservation measures are needed. Full article

Orthohantaviruses are mainly carried and transmitted by wild rodents, although during the last decade, they have also been identified in multiple species of shrews and moles. Orthohantavirus, Orthohantavirus seewisense (Seewis virus, SWSV), first detected in Switzerland in a single Sorex araneus (Eurasian common shrew) specimen, has been further described in several European countries, including Croatia’s neighboring Slovenia and Hungary. Croatia is a well-known endemic region for several zoonotic agents including three different orthohantaviruses: Orthohantavirus puumalaense (PUUV), Orthohantavirus dobravaense (DOBV), and Orthohantavirus tulaense (TULV). In this study, nine shrews were tested and SWSV RNA was detected in liver, lung, and kidney belonging to two shrews (22.22%), one collected on Medvednica mountain in Zagreb County, and the other in the Stara Gradiška area in lowland Croatia. The phylogenetic analysis of the complete S segment’s open reading frame (ORF) and partial L-segment revealed that the Croatian sequences, when compared to sequences from the adjacent geographic regions, form a specific genetic lineage. Two SWSV-positive shrew species—Sorex araneus and Neomys milleri (Mediterranean water shrew)—were identified using barcode-based sequence analysis. Therefore, the SWSV detection in N. milleri throughout the course of this study is seen as a rare find in this shrew species. To our knowledge, this is the first molecular and phylogenetic analysis of SWSV in Croatia. Full article

The origin and dynamics of a 2010 pluvial flood in the valley of a large European river are described. In order to study how local people perceive this catastrophic event a small administrative unit (rural municipality) within the Holocene floodplain (thus flooded to 90%) was chosen. Using a questionnaire a human-research survey was performed in the field among 287 people living in flood-prone areas. Almost half of the interviewees feel safe and do not expect a flood recurrence (interpreted as a levee effect). Seventeen percent believe the levee was intentionally breached due to political issues. Six percent of interviewees link the breach with small mammals using levees as a habitat, e.g., beavers, moles, and foxes. The sex and age of interviewees are related to these opinions. Most interviewees (39%) think that flooding was a result of embankment (dyke) instability. The spatial distribution of the survey results are analyzed. Maps presenting: inundation height, economic loss, attitude to geohazards and perception of possible flood recurrence were drawn. Causes of the flood as viewed by local inhabitants and in the context of the riverine geological setting and its processes are discussed. Particular attention is paid to processes linking the levee breach location with specific geomorphic features of the Holocene floodplain. A wide perspective of fluvial geomorphology where erosive landforms of crevasse channels (and associated depositional crevasse splays) are indicators of geohazards was adopted. This distinct geomorphological imprint left by overbank flow is considered a natural flood mark. Such an approach is completely neglected by interviewees who overestimate the role of hydrotechnical structures. Full article

The recent detection of both Nova virus (NVAV) and Bruges virus (BRGV) in European moles (Talpa europaea) in Belgium and Germany prompted a search for related hantaviruses in the Iberian mole (Talpa occidentalis). RNAlater^®-preserved lung tissue from 106 Iberian moles, collected during January 2011 to June 2014 in Asturias, Spain, were analyzed for hantavirus RNA by nested/hemi-nested RT-PCR. Pairwise alignment and comparison of partial L-segment sequences, detected in 11 Iberian moles from four parishes, indicated the circulation of genetically distinct hantaviruses. Phylogenetic analyses, using maximum-likelihood and Bayesian methods, demonstrated three distinct hantaviruses in Iberian moles: NVAV, BRGV, and a new hantavirus, designated Asturias virus (ASTV). Of the cDNA from seven infected moles processed for next generation sequencing using Illumina HiSeq1500, one produced viable contigs, spanning the S, M and L segments of ASTV. The original view that each hantavirus species is harbored by a single small-mammal host species is now known to be invalid. Host-switching or cross-species transmission events, as well as reassortment, have shaped the complex evolutionary history and phylogeography of hantaviruses such that some hantavirus species are hosted by multiple reservoir species, and conversely, some host species harbor more than one hantavirus species. Full article

Earlier, we demonstrated the co-circulation of genetically distinct non-rodent-borne hantaviruses, including Boginia virus (BOGV) in the Eurasian water shrew (Neomys fodiens), Seewis virus (SWSV) in the Eurasian common shrew (Sorex araneus) and Nova virus (NVAV) in the European mole (Talpa europaea), in central Poland. To further investigate the phylogeny of hantaviruses harbored by soricid and talpid reservoir hosts, we analyzed RNAlater^®-preserved lung tissues from 320 shrews and 26 moles, both captured during 1990–2017 across Poland, and 10 European moles from Ukraine for hantavirus RNA through RT-PCR and DNA sequencing. SWSV and Altai virus (ALTV) were detected in Sorex araneus and Sorex minutus in Boginia and the Białowieża Forest, respectively, and NVAV was detected in Talpa europaea in Huta Dłutowska, Poland, and in Lviv, Ukraine. Phylogenetic analyses using maximum-likelihood and Bayesian methods showed geography-specific lineages of SWSV in Poland and elsewhere in Eurasia and of NVAV in Poland and Ukraine. The ATLV strain in Sorex minutus from the Białowieża Forest on the Polish–Belarusian border was distantly related to the ATLV strain previously reported in Sorex minutus from Chmiel in southeastern Poland. Overall, the gene phylogenies found support long-standing host-specific adaptation. Full article

This multiproxy work presents the archeozoological analysis of fish and microvertebrate remains from the Middle Bronze Age tell site of Borsodivánka (Borsod Plain, North-eastern Hungary). The fish faunal assemblage provides valuable data on the choice of exploited consumption patterns, taphonomy, and aquatic paleoenvironmental conditions at the site during the Bronze Age. Only freshwater taxa are present in the assemblage, for example, northern pike (Esox lucius); cyprinids: roach (Rutilus rutilus), common carp (Cyprinus carpio), common chub (Squalius cephalus) and common nase (Chondrostoma nasus); and percids: European perch (Perca fluviatilis) and pikeperch (Sander lucioperca). Herpetofaunal and micromammal remains are also part of this study, improving our knowledge of the site’s freshwater ecosystem. The grass snake (Natrix cf. natrix) and the European pond terrapin (Emys orbicularis), typical of aquatic ecosystems, are associated with the Aesculapian ratsnake (Zamenis longissimus), more typical of forest, shrubland, and grassland. The presence of amphibians such as toads (Bufo/Bufotes sp.) and frogs (Rana sp.) complete the herpetofaunal list. The microvertebrates also support a mature fluvial system, as represented by taxa like the European water vole (Arvicola amphibius). Other micromammals are present, such as the wood mouse (Apodemus sylvaticus), the group of the common/field vole (Microtus arvalis/agrestis), the European mole (Talpa europaea), and the house mouse (Mus musculus). All of them are common in forests, shrubland, and grassland. However, the commensal house mouse is more commonly associated with anthropogenic areas. In conclusion, Borsodivánka is characterized by a diverse landscape mosaic, displayed by the co-existence of a well-developed forest and a freshwater inland ecosystem with agricultural land in the wider area. Finally, the Tisza River and its flood plain represented the main water source close to the site, distinguished by the dominance of fish species from deep and slow-flowing waters. Full article

Russia has a number of historical foci of zoonotic anthropogenic diseases. In Central Russia, the Republic of Mordovia is one of such areas, a region being known to have foci of haemorrhagic fever with renal syndrome (HFRS) and tularemia. It therefore requires continuous monitoring. The role of small terrestrial mammals as reservoirs of zoonoses has been previously proven for the region. The aim of this work is to take an integrated approach to assess the role of terrestrial and subterranean small mammals. Subterranean mammals are often not considered important reservoirs of zoonotic pathogens that cause human morbidity. Among small mammals in the wild environment, the bank vole, the yellow-necked mouse and the house mouse play important roles as vectors of zoonoses. Among wild subterranean mammals, the greater mole rat is important as a vector of tularemia and HFRS. We analyzed homogenized internal organs of these animals (lungs, spleen, kidneys). Of all samples from the greater mole rat, 83% were positive for tularemia antigens and 17% were positive forHFRS. None of the analyzed European moles had antigens of tularemia and HFRS. No double infection with both tularemia and hantavirus was detected in the subterranean mammals. Double infection was found among terrestrial mammals in the bank vole and the forest dormouse. Full article

The European mole (Talpa europaea) has a widespread distribution throughout Europe. However, little is known about the presence of zoonotic pathogens in European moles. We therefore tested 180 moles from the middle and the south of the Netherlands by (q)PCR for the presence of multiple (tick-borne) zoonotic pathogens. Spotted fever Rickettsia was found in one (0.6%), Leptospira spp. in three (1.7%), Bartonella spp. in 69 (38.3%) and Hantaviridae in 89 (49.4%) of the 180 moles. Infections with Anaplasma phagocytophilum, Babesia spp., Neoehrlichia mikurensis, Borrelia spp., Spiroplasma spp. and Francisella tularensis were not found. In addition, in a subset of 35 moles no antibodies against Tick-borne encephalitis virus were found. The obtained sequences of Bartonella spp. were closely related to Bartonella spp. sequences from moles in Spain and Hungary. The Hantaviridae were identified as the mole-borne Nova virus, with high sequence similarity to sequences from other European countries, and Bruges virus. Though the zoonotic risk from moles appears limited, our results indicate that these animals do play a role in multiple host-pathogen cycles. Full article

Anthropogenic carbon dioxide (CO₂) emissions from bottom-up inventories have high uncertainties due to the usage of proxy data in creating these inventories. To evaluate bottom-up inventories, satellite observations of atmospheric CO₂ with continuously improved accuracies have shown great potential. In this study, we evaluate the consistency and uncertainty of four gridded CO₂ emission inventories, including CHRED, PKU, ODIAC, and EDGAR that have been commonly used to study emissions in China, using GOSAT and OCO-2 satellite observations of atmospheric column-averaged dry-air mole fraction of CO₂ (XCO₂). The evaluation is carried out using two data-driven approaches: (1) quantifying the correlations of the four inventories with XCO₂ anomalies derived from the satellite observations; (2) comparing emission inventories with emissions predicted by a machine learning-based model that considers the nonlinearity between emissions and XCO₂. The model is trained using long-term datasets of XCO₂ and emission inventories from 2010 to 2019. The result shows that the inconsistencies among these four emission inventories are significant, especially in areas of high emissions associated with large XCO₂ values. In particular, EDGAR shows a larger difference to CHRED over super-emitting sources in China. The differences for ODIAC and EDGAR, when compared with the machine learning-based model, are higher in Asia than those in the USA and Europe. The predicted emissions in China are generally lower than the inventories, especially in megacities. The biases depend on the magnitude of inventory emissions with strong positive correlations with emissions (R² is larger than 0.8). On the contrary, the predicted emissions in the USA are slightly higher than the inventories and the biases tend to be random (R² is from 0.01 to 0.5). These results indicate that the uncertainties of gridded emission inventories of ODIAC and EDGAR are higher in Asian countries than those in European and the USA. This study demonstrates that the top-down approach using satellite observations could be applied to quantify the uncertainty of emission inventories and therefore improve the accuracy in spatially and temporally attributing national/regional totals inventories. Full article