Search Results (2,551)

The demand for functional dairy products is increasing, in response to the adverse correlation between high saturated fat consumption and cardiovascular health problems. The present study investigated the physicochemical and sensory features of a prototype of functional reduced-fat Mozzarella cheese fortified with inulin made from milk obtained by integrating the cattle diet with laurel essential oil (LEO). Two samples were compared over a 10-day storage period: a whole-milk Mozzarella cheese (MC), and a reduced-fat Mozzarella cheese fortified with 10% (w/v) of inulin (MI). The results show that incorporating inulin during the stretching phase required more time (2.55 min longer) to obtain the final product. However, in addition to a 5% fat decrease, the MI cheese achieved an inulin content of 3.31%, satisfying the European Regulation No 1924/2006, for the “Source of Fiber” claim. On the other hand, from a nutritional perspective, the dietary LEO integration significantly modulated the lipid fraction of the sample, resulting in a 40% increase in monounsaturated fatty acids (MUFAs) and a marked enrichment in polyunsaturated fatty acids (PUFAs). Considering the texture attributes, the incorporation of inulin during the stretching phase led to the formation of a micro-gel that acted as a functional filler, resulting in significantly higher hardness (33.41 N for MI and 16.10 N for MC), throughout the 10-day storage period. Temporal Check-All-That-Apply (TCATA) analysis confirmed that while the MI sample introduced vegetable and cooked milk notes, MI maintained major textural integrity throughout the shelf-life. These findings demonstrate that the synergy between inulin fortification and dietary laurel essential oil supplementation represents a highly effective strategy for producing reduced-fat pasta filata cheeses. This dual approach not only preserves sensory and textural integrity but also yields a high-value functional product characterized by an optimized fatty acid profile and a significant fiber intake. Full article

Cervical cancer (CC) is an alarming global health problem, with predominantly higher incidence, lethal progression, and mortality among women of African ancestry (AA) than women of European ancestry (EA). Although persistent high-risk human papillomavirus (HPV) integration and infection are the key etiological factors, currently available evidence implicates epigenetic reprogramming as a prime contributor to ancestry-associated differences in CC pathogenesis. To address these disparities, we performed genome-wide DNA methylation profiling of HPV-positive cervical intraepithelial neoplasia (CIN) lesions from AA (n = 15) and EA (n = 15) women. Differential methylation analysis identified a distinct epigenomic landscape in AA-CIN lesions, with widespread hypermethylation and hypomethylation at promoter-associated and regulatory CpG sites. Pathway enrichment analyses highlighted dysregulation of ECM-receptor interaction, focal adhesion, PI3K-Akt, MAPK, Ras, Rap1, and RUNX-dependent transcriptional networks. Comparative analysis across CIN grades (CIN1–CIN3) revealed progressive epigenetic reprogramming affecting cell cycles, cytoskeletal dynamics, signaling, and metabolic pathways. Among hypermethylated tumor suppressor genes, SH3GL2 and ARHGAP25 showed significantly higher methylation in AA lesions, accompanied by concomitant loss of their protein expression. MBD1, a methylation-binding regulator, was upregulated in AA-CIN lesions, coinciding with global loss of 5-hydroxymethylcytosine (5hmC), suggesting enhanced transcriptional repression. In contrast, EA lesions retained protein expression and 5hmC levels. Collectively, these findings indicate that early, ancestry-specific epigenetic modifications target tumor suppressor pathways and converge on oncogenic signaling, cytoskeletal remodeling, and cell–cell adhesion. Our study provides mechanistic insight into CC health disparities, identifying SH3GL2 and ARHGAP25 hypermethylation as potential biomarkers, and highlighting epigenetic regulation as a contributor to disparate CC progression in AA women. Full article

Livestock farming represents a key economic activity in the Tataouine Governorate of southern Tunisia, where cattle and dromedary camels coexist. Varicellovirus bovinealpha1 (BoAHV-1), the etiological agent of infectious bovine rhinotracheitis (IBR), primarily affects cattle, while its circulation in camelids remains poorly understood. Following recent European Union regulations requiring BoAHV-1 surveillance in multiple animal species, this short communication reports serological findings from dairy cattle and dromedary herds in southern Tunisia. In March 2024, serum samples were collected from four non-vaccinated farms, including two intensive Friesian dairy cattle herds and two extensive dromedary herds (50 animals each). Serum samples from all animals were tested for BoAHV-1 antibodies using competitive commercial gB- and gE-based enzyme-linked immunosorbent assays (c-ELISA) and confirmed by virus neutralization test (VNT). Antibodies against BoAHV-1 were detected in cattle from both dairy farms, with low seroprevalence and neutralizing antibody titers, indicating past or ongoing exposure. In contrast, all dromedary samples tested seronegative by both c-ELISA and VNT. These findings confirm BoAHV-1 circulation in cattle in the Tataouine region and its absence in dromedaries at sampling. Further studies involving larger sample sizes and molecular investigations are required to clarify the potential role of camelids in BoAHV-1 epidemiology in southern Tunisia. Full article

European airspace is currently facing significant challenges due to increasing traffic demand and limited sector capacity. This situation leads to an overload of demand, so Air Traffic Controllers (hereinafter ATCOs) are often forced to implement regulations that cause delays. Moreover, an ATCO cannot be endorsed in an unlimited number of sectors, as doing so would compromise the maintenance of operational proficiency and specific sector skills. Consequently, the limited cross-sector flexibility of controllers has become a key constraint in optimizing airspace management. Additionally, the strategic definition of sector groups has a direct impact on which sector configurations can be activated. An inadequate sector grouping strategy may hinder operations by restricting access to more efficient sector configurations. While in some cases, controllers may be endorsed for multiple sectors (up to ten), this flexibility remains insufficient to mitigate capacity and efficiency challenges fully. IFAV3 (Increased Flexibility of ATCO Validation En-Route) project has been developed within the Single European Sky ATM Research (hereinafter SESAR) framework, aiming to maximize flexibility in ATCO rostering. Its main expected benefits include an improvement in cost efficiency in Air Traffic Control (hereinafter ATC) through reduced training costs and optimized rostering by a better utilization of existing capacity. Full article

The increasing use of pressure-sensitive labels (PSLs), driven by growth in the packaging sector, raises concerns regarding material consumption and end-of-life management under evolving European packaging regulations. This study investigates the biodegradation potential of sustainable PSL facestocks produced from 15% agro-industrial by-products, 40% post-consumer recycled fibers, and 45% virgin wood pulp. Their biodegradation behavior was compared with bio-based polyethylene (PE) facestocks using laboratory-scale aerobic soil burial tests conducted for up to 28 days. Biodegradation was assessed through weight loss measurements, visual evaluation, Fourier transform infrared (FTIR) spectroscopy, and fluorescence analysis. Fiber-based facestocks exhibited significant degradation, reaching approximately 50–55% weight loss after 28 days, accompanied by structural changes in the cellulose matrix and reduced fluorescence intensity. In contrast, bio-based polyethylene facestocks showed negligible weight loss and only minor spectroscopic changes, indicating high stability under the tested conditions. The results demonstrate that fiber-based samples derived from agro-industrial and recycled sources possess substantially higher biodegradation potential than bio-based polymeric alternatives. These findings support the use of fiber-based PSL facestocks in applications requiring improved environmental compatibility. Full article

Modern cattle comprise two major evolutionary lineages: intensively selected commercial breeds and locally adapted native populations. To investigate their genomic divergence, we performed a comparative population genomic analysis by integrating whole-genome resequencing (WGS) data from multiple representative native breeds and major European commercial breeds. Population genetic analyses showed clear phylogenetic separation between the two groups, with distinct patterns of genetic diversity. Chinese native cattle exhibited generally higher nucleotide diversity (π), lower inbreeding levels, and geographically structured admixed ancestry. Comparative analyses of selection signatures identified 886 candidate selected genes in European commercial breeds, which were primarily enriched in pathways related to production traits, including protein turnover, reproductive regulation, lipid metabolism, and neuro-regulation. In contrast, 50 candidate selected genes in Chinese native cattle were significantly enriched in nervous system functions, particularly ligand-gated ion channel activity and chloride transport (e.g., GRID2, GLRA2/4, GABRD), suggesting neural/ionic regulation may contribute to local adaptation alongside other polygenic mechanisms. Additionally, the two groups also differed in patterns of deleterious mutation load. These findings indicate partially distinct evolutionary trajectories between “production-optimized” and “environment-adapted” cattle and highlight the value of conserving the genetic diversity and adaptive alleles of Chinese native cattle. Full article

Public participation in planning, though a foundational democratic principle, faces persistent implementation challenges across diverse planning systems. This paper examines participatory planning practice in Ireland and Serbia—two countries representing distinct planning traditions (discretionary and conformance-based, respectively) yet confronting shared structural pressures. Through comparative analysis of four local land use planning instruments (the Development Plan and Local Area Plan in Ireland; the Municipal Spatial Plan and General Regulation Plan in Serbia), the study investigates how institutional design and legislative frameworks shape the depth and quality of participatory practice. Methodologically, the research triangulates statutory regulations, public hearing documentation, and non-statutory participation records across two planning scales (county/municipal and local/sub-municipal). A four-dimensional analytical framework—informing, consultation, collaboration, and monitoring—guides the systematic comparison of participatory mechanisms across the selected cases. Findings reveal that, while both systems remain predominantly at the informing and consultation levels, critical differences emerge in how participation is structured and documented in institutional practice. Ireland’s discretionary system enables multi-channel information dissemination, feedback-oriented consultation, and non-statutory collaborative experimentation beyond legal minimums. Serbia’s conformance-based system confines participation largely to statutory procedures, with objection-based consultation and limited collaborative mechanisms, though distinctive features, such as the public hearing session, provide direct opportunities for deliberation absent in the Irish context. The study contributes to European comparative planning scholarship by demonstrating that participatory depth is shaped less by the formal existence of legal provisions than by the interplay between institutional design, procedural arrangements, transparency, and responsiveness. Full article

Biogenic amines (BAs) are important qualitative indicators of quality, as they are produced by specific microbial strains and can therefore reflect the activity of specific spoilage organisms (SSOs). Their presence in food, including wine, provides valuable information on processing conditions, hygiene practices and storage management throughout the production chain. In wine, the accumulation of BAs—particularly histamine, tyramine and putrescine—is mainly associated with microbial activity during fermentation, especially malolactic fermentation, and may pose potential risks to consumer health. Despite the recognized toxicological relevance of BAs, current European Union (EU) regulations only establish limits for histamine in certain fish products, with no specific legal thresholds defined for wine. However, growing evidence on the interactions and adverse effects of BAs highlights the need to better address their occurrence in wine and to improve consumer awareness regarding safety and quality aspects. In addition to safety concerns, the implementation of good hygiene and manufacturing practices across the entire production process plays a crucial role in controlling BA levels in the final product. These factors, together with the intrinsic characteristics of wine, may influence consumer perception and choice, integrating aspects of health, production methods and product quality. Recent findings suggest a shift in perspective, where BAs are not only considered risk markers but also useful indicators for assessing wine quality and enhancing consumer safety. Full article

This paper examines how scam compounds, money mules and crypto-assets operate as interdependent elements of contemporary money-laundering chains. It assesses whether existing anti-money laundering (AML) and crypto-asset regulatory frameworks are capable of disrupting these chains holistically, rather than addressing individual components in isolation, with particular reference to scam-compound activity in Southeast Asia. The study adopts a qualitative comparative case-study methodology grounded in legal and regulatory analysis. Four empirically grounded cases are examined: two Southeast Asian scam-compound enforcement cases (Cambodia and Myanmar) and two European crypto-asset seizure cases (Ireland and Italy). Judicial decisions, enforcement actions and regulatory instruments are analysed through a chain-based analytical framework aligned with Financial Action Task Force (FATF) standards, the EU Markets in Crypto-Assets Regulation (MiCA) and the Anti-Money Laundering Authority (AMLA) framework. The analysis reveals a structural divergence in enforcement strategies: Southeast Asian responses increasingly prioritise network- and infrastructure-level disruption of scam compounds, whereas European approaches remain largely centred on post-offence crypto-asset seizure through traditional proceeds-of-crime mechanisms. Across all jurisdictions, money mules emerge as a critical yet systematically under-regulated intermediary layer enabling the resilience of crypto-laundering operations. The paper advances existing AML typologies by conceptualising scam compounds, money mules and crypto-assets as interconnected components of a single crypto-laundering chain. This chain-based perspective offers a novel analytical and regulatory lens for understanding organised crypto-enabled fraud. The study is based on a qualitative, case-based design and does not aim for statistical generalisation. However, the analytical framework developed is transferable to other jurisdictions experiencing similar scam-compound and crypto-laundering dynamics. The findings suggest that effective AML enforcement requires coordinated intervention across multiple nodes of the laundering chain, including scam compound infrastructure and money mule networks, alongside traditional asset-seizure mechanisms and CASP supervision. By highlighting the structural links between scam compounds, coercive labour and crypto-laundering mechanisms, the paper underscores the broader social harms of crypto-enabled fraud and the need for integrated regulatory responses that address both financial crime and human exploitation. Full article

Smart contracts raise persistent challenges regarding compliance with traditional contract formalities, including writing, signature, notarization, and in certain transactions, registration. These issues are particularly significant in high-value and public-facing transactions such as real estate, where formalities determine legal validity, evidentiary sufficiency and publicity effects. While existing scholarly work has examined these challenges from either doctrinal or technological perspectives, limited attention has been given to how the functional roles of formalities interact with blockchain architecture, practitioner perceptions and institutional legal frameworks. This study addresses this gap through a mixed-methods approach combining doctrinal legal analysis with qualitative socio-legal research based on 27 semi-structured interviews with legal professionals including attorneys, judges, and academic scholars. The analysis is grounded in a civil law framework, with particular reference to the Jordanian legal system, while references to the European Union’s eIDAS Regulation are used illustratively to demonstrate regulatory approaches to digital authentication. The findings demonstrate that blockchain-based systems can effectively support the evidentiary and attribution functions of contractual formalities through cryptographic verification, consensus mechanisms, and automated execution. However, they do not independently satisfy formalities that perform cautionary, constitutive, protective or public order function, namely notarization and registration, which remain dependent on institutional validation and legal recognition. The analysis further shows that practitioner concerns reflect not only doctrinal constraints but also institutional roles and varying levels of technical familiarity. To address these limitations, the study proposes a function-based analytical framework for evaluating smart contract formalities and identifies two complementary pathways for legal adaptation: (i) institutional integration, including registry-linkage systems and hybrid contracts; and (ii) technological adaptation, including digital authentication frameworks and legal oracles that connect on-chain execution to off-chain legal conditions. The study concludes that smart contract formalities’ challenges arise not solely from technological limitations, but from the interaction between legal doctrine, institutional structures, and system design. It advances a functional framework for aligning automation with the evidentiary, protective, and publicity functions of contractual formalities. Full article

The European Regulation (UE) 432/2012 includes a specific health claim for olive-oil-associated with its phenolic content, which is based on its protective role against lipid oxidation in the blood. To make use of the health claim, olive oil must have a minimum concentration in phenolic compounds of 250 mg/kg. Reviewing the health claim, the phenolic compounds referred to are the secoiridoid derivatives of hydroxytyrosol and tyrosol. A method based on absolute quantification of phenolic compounds in olive oil is proposed for the recognition of the health claim. The method involves liquid–liquid extraction with a 1:8 (v/v) oil:extract ratio to avoid saturation of the extract in oils with a higher phenolic content and its subsequent determination through LC–MS/MS in multiple reaction monitoring (MRM) mode, the gold standard technique in many application fields because of its analytical features. The optimized method was applied to a set of 100 extra virgin olive oils (EVOOs), and the results obtained were compared with the classic Folin–Ciocalteu method. The comparison between the two methods showed that the classic method is a non-selective method that can be affected by many interferences and that the Folin method underestimates the real phenolic content. Full article

The European Union’s Carbon Border Adjustment Mechanism (CBAM), the world’s first system to impose tariffs on the carbon emissions of imported products, commenced its transition period in October 2023 and is scheduled for full implementation in January 2026. This mechanism exerts a profound impact on the global trade landscape and corporate environmental management practices. Taking the CSI All Share Index constituent companies as a research sample, this paper empirically evaluates the impact of the CBAM transition period on the environmental scores of Chinese export enterprises utilizing the Propensity Score Matching Difference-in-Differences (PSM-DID) method. The results indicate that the CBAM transition period significantly inhibits the short-term environmental performance of regulated enterprises. Mechanism analysis reveals that increased financing constraints serve as a core mediating channel, wherein escalated compliance costs and compressed cash flows crowd out resources for low-carbon investments. Furthermore, heterogeneity analysis demonstrates that the negative impact is more pronounced among state-owned enterprises, firms with lower audit quality, and firms with a higher proportion of female executives. Accordingly, the study recommends establishing targeted green transition financing mechanisms, accelerating domestic carbon market reforms, and strengthening international technical harmonization to build corporate resilience against global climate governance shocks and promote sustainable growth. Full article

Today, financial institutions’ architecture does not rely on one single technology. Instead, it uses a multi-technology approach in order to cover modern requirements and, at the same time, remain relevant. It integrates technologies such as relational databases, Big Data for analysis, and Cloud environments for distributed capacities within a complex data architecture. At the same time, due to European data governance regulations, governance mechanisms such as encryption, pseudonymization, and incremental versioning must be applied on each architectural layer in order to comply with strict European governance rules. In this study, the impact of data governance is assessed by applying these mechanisms from the data-ingestion level, using diverse data types such as structured, semi-structured, and unstructured data, across relational databases, Big Data analysis, and Cloud distributed systems. In doing so, metrics such as execution time, CPU, and memory usage are assessed in order to properly evaluate the impact of governance mechanisms on financial systems. The results show that governance can be successfully integrated, provided these mechanisms are embedded at the architectural level, ensuring that performance, scalability, and compliance are maintained across the entire processing pipeline. Full article

Flexible factories and adaptive fabrication lines offer a testbed for advanced multimodal interaction concepts that can support the inclusion of disabled workers in Industry 5.0 manufacturing systems. The study synthesizes interdisciplinary data from ergonomics, industrial automation, and EU regulatory frameworks to establish a conceptual model for human-machine interaction. Building on conceptual modeling and a structured literature analysis, the study proposes a six-step integration framework that links task demands, worker capabilities, and interaction modalities within human-in-the-loop manufacturing environments. Although no empirical case study was conducted in this phase, an exemplary application is presented for a semi-automated bike wheel manufacturing process. Detailed machine-based assembly line flows and simulated process data were utilized for illustrative purposes to depict the process and validate the proposed Capability–Task Matching Matrix. The results operationalize the human-centric vision of Industry 5.0 by providing a structured methodology for the inclusion of disabled workers within fabrication environments. The findings are organized into two primary components: the conceptual development of the Integration Approach and its practical application to a semi-automated industrial use-case. Finally, a particular focus is placed on Brain–Computer Interfaces (BCIs) as an emerging interaction channel that enables non-muscular control, attention monitoring, and neuroadaptive feedback, complementing conventional interfaces rather than replacing them. The framework is illustrated through application to the same semi-automated bicycle wheel assembly line, where BCI-supported interaction, augmented interfaces, and robotic assistance are mapped to specific production tasks and assessed in terms of feasibility and technological maturity. Drawing on the paper’s results, an explanatory 10-year roadmap outlines the feasibility and phased deployment of BCI solutions. It aligns technological advances with European regulations and a vision for a fully inclusive manufacturing enterprise. Full article

Selenium (Se) is an essential trace element. The bioactivity of Se arises from its incorporation into the 21st amino acid, selenocysteine (Sec). Twenty-five human genes have been identified that encode selenoproteins, each of which contains at least one Sec residue. Selenoprotein functions include antioxidant responses, thyroid hormone synthesis, and maintenance of cellular redox homeostasis. Due to its role in critical cellular functions, Se deficiency is associated with morbidities of the cardiovascular system and connective tissue in regions of countries with low soil Se content. While these morbidities are geography-specific and have been mitigated in adults through public health interventions, preterm infants remain susceptible to Se deficiency worldwide. Infants born preterm are deprived of fetal Se accrual in the 3rd trimester of pregnancy, a deficiency compounded by higher Se needs than term infants and older infants and dependence on parenteral nutrition (PN) and fortification. In addition, the composition of selenoproteins and selenometabolites in human milk is different from that in formula and PN, yet little is known about the biological impact of these differences. The knowledge gap in optimal Se supplementation is reflected in discrepant guidelines between North American and European/Chinese nutrition societies, whose recommended Se supplementation in preterm infants differs by more than 2-fold. In this review, we describe the biosynthesis, metabolism, and maternal-fetal transfer of Se. In addition, we address how developmentally regulated aspects of metabolism may impact how preterm infants respond to supplementation with different forms of Se. Lastly, we highlight current challenges and recommendations for optimizing Se levels in neonates based on available data. Full article