Search Results (886)

Background/Objectives: Multiple Traffic Light (MTL) front-of-pack (FOP) labels are being considered in Bahrain. We tested whether an adapted MTL label improves the nutritional quality of grocery purchases. Methods: In a two-arm randomized controlled intercept trial (January–May 2025), adults (≥21 years) responsible for household grocery shopping were recruited in high-footfall public venues and asked to complete a one-time shop on a tablet-based, purpose-built online grocery platform. The MTL label was adapted for Arabic reading direction and displayed per-serving nutrients and % recommended daily intake. Treatment effects were estimated using ordinary least squares regressions with robust standard errors and covariate adjustment. Results: Of 395 randomized participants, 360 were included in primary analyses (control n = 183; MTL n = 177). MTL exposure was not associated with a significant change in the primary outcome (basket weighted average MTL score per serving; β = 0.037; p = 0.64) or in per-serving calories and nutrients of concern (all p > 0.17). In the post-shop assessment, only 47.2% of participants correctly interpreted MTL labels, indicating modest objective label comprehension under the study conditions. Conclusions: These findings suggest that the impact of front-of-pack labels likely depends on both implementation features and consumer understanding, and that pairing labels with public communication and nutrition literacy initiatives may be necessary to maximize the effectiveness of labels in Bahrain and the wider Gulf region. Full article

Data on arsenic (As) and other potentially toxic elements (PTEs) in Pakistani retail meats are limited, constraining evidence-based dietary risk assessment and management. This study aimed to determine the concentrations and profiles of As and seven other PTEs (Cr, Ni, Mn, Pb, Cd, Cu, Zn) in commonly consumed meats and to evaluate the associated non-carcinogenic health risks. Ninety-two paired liver and muscle samples from broiler chicken, goat (mutton), and beef cattle were collected from four cities across the Indus Plain and analyzed using inductively coupled plasma mass spectrometry (ICP-MS). Dietary exposure was evaluated using estimated daily intake (EDI), target hazard quotient (THQ), and hazardous index (HI) under typical and high-consumption scenarios. Overall, Zn and Cu exhibited the highest concentrations, followed by Mn and Cr, whereas As, Pb, Ni, and Cd occurred at comparatively lower but environmentally relevant levels. Beef liver exhibited the highest contamination levels, exceeding FAO/WHO permissible limits for Pb, Cu, and Cd in up to 40% of samples. In contrast, mutton and beef muscle contained the highest As and Zn concentrations, while chicken muscle showed elevated Cr levels. Multivariate statistical analysis revealed three dominant co-variation patterns, suggesting potential contamination pathways: (i) geogenic groundwater sources enriched with As, Cr, and Ni; (ii) atmospheric and industrial dust inputs linked with Pb, Cd, and Mn; (iii) mineral-enriched feed additives potentially contributing to elevated Zn and Cu, particularly in poultry. Under high-consumption scenarios, THQ values for As, Cr, Cu, and Zn exceeded the safety threshold (THQ > 1), highlighting beef products as the dominant source of chronic dietary risk. Overall, the findings highlight pronounced tissue- and species-specific accumulation trends, and emphasizes the urgent need for stricter feed and water quality control measures to minimize dietary exposure to PTEs. Full article

Background/Objectives: Nutrient profiling models are widely used to support healthier food choices, but their applicability may be limited in dietary cultures with multi-dish meals and high consumption of minimally processed foods. This study extended the Ajinomoto Group Nutrient Profiling System (ANPS), originally developed for dish- and meal-level assessment, to evaluate overall quality of daily intake (ANPS-Day) based on four components (protein, vegetables, saturated fatty acids [SFAs], and sodium), and examined its criterion-related validity using 24 h urinary biomarkers. Methods: A total of 324 healthy Japanese adults aged 20–69 years completed four-day semi-weighed dietary records and two non-consecutive 24 h urine collections. Urinary sodium, potassium and urea nitrogen were measured. Associations were examined using age- and sex-adjusted Spearman correlation coefficients and trend analyses. Results: The crude ANPS-Day score showed weak and inconsistent correlations with urinary biomarkers. In contrast, the energy-adjusted ANPS-Day score was positively correlated with estimated potassium intake (r = 0.25) and inversely correlated with the urinary sodium-to-potassium (Na/K) ratio (r = −0.24). In quartile analyses, higher energy-adjusted ANPS-Day scores were associated with higher protein and potassium intakes and with a lower Na/K ratio (all p for trend ≤ 0.001). In component analysis, vegetable points were positively associated with potassium intake, whereas sodium points were inversely associated with estimated sodium intake and the Na/K ratio. SFA points were not associated with urinary biomarkers. Conclusions: The energy-adjusted ANPS-Day score showed modest but biologically plausible associations with urinary biomarkers, providing partial evidence of criterion-related validity in assessing diet quality in multi-dish dietary settings. Full article

Plastics are now ubiquitous in the environment, in this, the “Plasticene” era. Microplastics (MPs) and Nanoplastics (NPs) are emerging contaminants of global concern. This systematic review, registered on PROSPERO and conducted according to PRISMA guidelines, investigated the presence, distribution, and characteristics of MPs in tap water (TW) worldwide, and estimated the population’s Estimated Daily Intake (EDI) by age group, including pregnant women. A comprehensive search across PubMed, Scopus, Web of Science, and Cochrane identified 22,650 records, of which 8 studies were included. MPs were detected in treated water (TW) in the studies included in this review, although the currently available evidence remains limited. Calculated EDIs were highest in children aged 6 months to 3 years (up to 39 MPs/kg bw/day), followed by pregnant women (up to 14.96 MPs/kg bw/day), reflecting differences in water intake per body weight. These estimates must be interpreted as indicative, estimated by methodological variability among studies. The widespread presence of MPs in TW calls for standardized methods, improved treatments, and thorough monitoring to assess risks and protect public health. Full article

Background/Objectives: Dairy yogurts are a source of protein and micronutrients in the Canadian diet. However, Canada’s Food Guide emphasizes the consumption of plant-based foods, which is facilitated by a greater availability of dairy alternatives on the market. The nutritional composition of these products varies and can differ from dairy foods such as yogurt, which contain high-quality protein and micronutrients. The objective of this study was to examine the effect of dairy yogurt consumption as part of a diet on any given day on nutrient intakes in Canadians across ages. Methods: The 2015 Canadian Community Health Survey (CCHS)—Nutrition first day 24 h recalls of males and females > 1 years of age (n = 17,308) and of yogurt consumers (n = 3788) were examined to estimate nutrient intakes arising from yogurt consumption. Respondents were allocated into four groups defined by their daily yogurt intake in grams (i.e., Group I/non-yogurt consumers: <1 g; Group II: 1–90 g; Group III: 90–115 g; Group IV: >115 g). Results/Conclusions: The results of this study provide timely data on Canadian yogurt consumption across the ages and show that those consuming yogurt have higher intakes of essential nutrients, such as protein, calcium, potassium, vitamin D, and dietary fibre. The data from this study emphasize the importance of yogurt in the context of a healthy eating pattern and emphasize the need to encourage consumption of yogurt within Canada’s Healthy Eating Strategy. Full article

This study evaluates the accumulation of trace elements (Al, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Cd, Hg, and Pb) in the muscle tissues of six commercially important fish species (Scophthalmus maximus, Merlangius merlangus, Mullus ponticus, Trachurus mediterraneus, Engraulis encrasicolus, and Sprattus sprattus) harvested from multiple locations across the Sinop and Samsun coasts of the southern Black Sea during the 2023–2025 fishing seasons to assess potential human health risks. Element concentrations were quantified using inductively coupled plasma mass spectrometry (ICP-MS) and compared against national and international food safety standards. Results indicated that essential elements, particularly Fe and Zn, exhibited the highest concentrations, while Co and potentially toxic elements (Cd, Pb, As, and Hg) remained at lower levels. Although significant geographical variations in accumulation were observed between sampling locations, inter-species differences were relatively minor. Human health risk assessments, including Estimated Daily Intake (EDI), Target Hazard Quotient (THQ), and Carcinogenic Risk (CR), revealed that all THQ values were well below 1, indicating no non-carcinogenic concerns. Furthermore, CR values for As, Cr, and Pb fell within the acceptable range (10⁻⁶ to 10⁻⁴) defined by the U.S. EPA. Consequently, fish consumption from these regions poses no unacceptable risk, though localized element elevations suggest a need for enhanced environmental monitoring of pollution sources. Full article

Background: The 2025–2030 Dietary Guidelines for Americans recommend that 6–12-month-old infants receive 11 mg iron/day. The contribution of iron-rich foods in meeting guidelines is unclear. Objectives: The aims were to: (1) determine the contribution of iron-fortified cereal, infant formula and heme-iron sources to infants’ total dietary iron intake; (2) examine differences in iron adequacy by milk-feeding type; and (3) identify feeding patterns associated with meeting daily iron requirements through dietary sources. Methods: Mothers of infants were recruited from a pediatric clinic and 24 h feeding recalls were conducted to estimate infants’ iron intake. Infants’ milk-feeding types were: breastmilk only (BF), mixed (MF), or infant formula only (FF). Main outcomes were: meeting/not meeting daily iron requirement (11 mg) overall and by milk-feeding type; contribution of iron-fortified infant cereal, formula and meat to daily iron intake. Descriptive statistics, bivariate chi-square tests, and multivariate logistic regression analyses were conducted. Results: Most participants identified as African American or Hispanic (76%) and were enrolled in the Special Supplemental Nutrition Program for Women, Infants, and Children (84%). Thirty-nine percent consumed < 11 mg iron/day from dietary sources. By milk-feeding type, inadequate iron intake was significantly higher among the BF (72%) and MF (74%) groups vs. the FF group (24%, p < 0.05). Iron-fortified cereals were consumed by 46% of infants and provided a median iron intake of 6.75 mg. Among the FF group, infant formula provided 63% of the daily iron requirement. Conclusions: Inadequate dietary iron intake is common. Iron-fortified cereal is an important dietary iron source. Future research is warranted to understand the relations among infants’ daily iron intake, iron sources (heme vs. non-heme), and iron status. Full article

Levels of glyphosate and their metabolite AMPA were measured in urine of farmers and their spouses that lived in intensively farmed area in Morocco. The levels were used as proxies to determine the exposure of these target population to herbicides. Determinants of exposure to glyphosate were also assessed. Urine was collected from 104 farmers, 50 spouses and 50 people from the general adult population and information from sociodemographic characteristics and occupational exposure was collected from questionnaires administrated to farmers and spouses. The detection frequency was 67% for glyphosate and 62% for AMPA among farmers, and 56% for glyphosate and 48% for AMPA among spouses, with the mean concentration of 1.22 μg L⁻¹ and 0.85 μg L⁻¹ among farmers, and 0.58 μg L⁻¹ and 0.50 μg L⁻¹ among spouses, respectively. Nevertheless, mean values of glyphosate and AMPA for general population were below the reported quantification limits. Multiple regression analysis showed that smoking status, applying glyphosate in the last 7 days, and glove use are the most important contribution to urinary levels of glyphosate and AMPA among farmers, and proximity of home to spraying area, and herbicides drift enters house are the main predictors of urinary glyphosate and AMPA exposure among spouses. The GMs of estimated daily intake were 1.26 and 1.39 µg/kg BW/day for glyphosate and AMPA among farmers, and 0.56 and 0.96 µg/kg BW/day for glyphosate and AMPA among spouses, respectively. This study provides further evidence on factors associated with glyphosate and AMPA exposure, especially in developing countries. Full article

Potentially toxic elements (PTEs) in mining-impacted wetlands can transfer from soil and water to forage and grazing livestock, resulting in dietary exposure for nearby communities. In this study, arsenic (As), lead (Pb), and cadmium (Cd) were quantified in key environmental matrices (soil, surface water, and forage) and in sheep tissues (liver, kidney, and muscle) from six georeferenced grazing sites in the Ite coastal wetland (Tacna, Peru) during the dry season. Samples were acid-digested following U.S. EPA protocols and analyzed by atomic absorption spectrometry (AAS) under QA/QC procedures (certified reference materials, blanks, duplicates, and matrix spikes); matrix-specific detection and quantification limits are reported. Arsenic dominated the contamination profile (forage: 428.6 mg kg⁻¹, dry weight; soil: 48.61 mg kg⁻¹; water: 0.97 mg L⁻¹) and was detected in sheep tissues (kidney: 0.1577 mg kg⁻¹; muscle: 0.1538 mg kg⁻¹; liver: 0.0644 mg kg⁻¹). Lead and cadmium were <LOQ in muscle and liver but were measurable in kidney (Pb: 0.0415 mg kg⁻¹; Cd: 0.0011 mg kg⁻¹). To support the interpretation of trophic transfer, screening transfer/bioaccumulation metrics (soil-to-forage, forage-to-tissue, and water-to-tissue) were calculated. Human dietary exposure was screened using estimated daily intake (EDI), hazard quotient (HQ), and margin of exposure (MOE). Because arsenic speciation was not measured, inorganic arsenic (iAs) risk was evaluated as a conservative upper bound (100% iAs) and through sensitivity scenarios (10–50% iAs). Under a high-meat-consumption scenario (300 g day⁻¹), the upper-bound assumption yielded HQ = 2.2 and MOE = 0.46; however, scenario analyses indicate that risk conclusions are highly dependent on the assumed iAs fraction. Overall, the results identify arsenic as a priority contaminant and support targeted grazing management, the provision of low-arsenic water sources, and remediation and monitoring actions to reduce exposure in vulnerable rural communities. Full article

The chemical composition of basil essential oil is influenced by plant developmental stage, which alters the relative distribution of volatile constituents and their functional properties. In this study, we investigated developmental-stage-dependent changes in the essential oil composition of Ocimum basilicum and evaluated their relationship with nitric oxide (NO) inhibitory activity and estragole exposure. Essential oils were obtained by hydrodistillation and analyzed by gas chromatography–tandem mass spectrometry (GC–MS/MS), resulting in the identification of 54 volatile compounds representing 98.13–98.97% of the total composition. Estragole remained the dominant constituent, ranging from 70.58% to 85.55%, with the lowest proportion at the flowering stage (Day 85). In contrast, minor constituents, including eucalyptol (2.41–3.77%), β-ocimene (0.52–1.98%), and methyleugenol (~2.00%), increased during flowering. NO inhibitory activity in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages was strongest at Day 85. Estimated Daily Intake (EDI) ranged from 0.4215 to 1.1005 µg kg⁻¹ bw day⁻¹, and Margin of Exposure (MOE) ranged from 2999 to 7830. These findings indicate that developmental stage influences composition, activity, and exposure. From a compositional perspective, the observed redistribution among major chemical groups reflects a structured balance that can be interpreted within a symmetry-related framework in multicomponent systems. Full article

Biogenic amines are nitrogenous compounds that may occur in foods through plant metabolism, bee enzymatic activity, or microbial decarboxylation. This study evaluated biogenic amines content in monofloral honeys from Algeria and Morocco, integrating compositional analysis, quality indices, and dietary exposure assessment within a toxicological risk characterization framework. Eight amines were quantified by HPLC-FLD, and Estimated Daily Intake (EDI) was calculated under adult and pediatric low- and high-consumption scenarios. Composite indices, including Total Biogenic Amines (TBA), Biogenic Amine Index (BAI), Vasoactive Amine Load (VAL), Potentiation Index (PI), and Quality Index (QI), were determined. Marked intra- and inter-city variability was observed, particularly for serotonin, tryptamine, and tyramine. Algerian Euphorbia orientalis L. samples showed the highest TBA and VAL values. However, histamine concentrations generally remained below 1 mg/kg, and tyramine levels were markedly lower than doses associated with hypertensive effects. Worst-case EDI values were in the order of 10⁻³–10⁻⁴ mg/kg body weight/day, including high-consumption pediatric scenarios. PI values were low, indicating limited synergistic amplification by diamines. Overall, despite botanical and geographical variability, the analyzed honeys exhibit a wide safety margin and based on the applied screening-level assessment, no immediate risk is indicated under the considered scenarios. Full article

Background: Accurate estimation of water and energy requirements is fundamental for establishing dietary reference values in young adults. However, evidence integrating objectively measured energy expenditure with detailed water turnover components remains limited in Chinese populations. Objectives: To quantify water intake, water loss, and energy expenditure in healthy young college students, and to examine how energy metabolism is associated with specific components of water turnover under free-living conditions. Methods: Twenty-one healthy adults aged 18–25 years participated in a 14-day observational study conducted in Beijing, China. Total energy expenditure (TEE) was measured over 14 days using the doubly labeled water (DLW) method. Physical activity was monitored over 7 consecutive days using a triaxial accelerometer. Water intake was assessed using multiple methods: water from beverages (including plain drinking water and other beverages) was recorded over 7 days using 24 h fluid intake records, while water from food was measured during days 5–7 using weighed food records combined with duplicate portion and direct drying methods. Urinary and fecal water loss were quantified using 24 h collections conducted during days 5–7. Metabolic water production and insensible water losses were estimated using established physiological equations. Multivariable linear regression analyses were conducted to examine associations between energy-related variables and components of water turnover. Results: Mean total daily water intake was 3023 mL, with water from beverages accounting for 54.1%, water from food for 36.7%, and metabolic water for 9.1%. Mean total daily water loss was 1931 mL, predominantly from urinary excretion (81.0%). DLW-measured TEE averaged 2018.6 kcal/day and was higher in males than in females. Most regression models examining total water intake and beverage-derived water were not statistically significant, and no consistent associations were observed between these variables and total energy intake, TEE, or PAEE. In contrast, TEE was positively associated with metabolic water production and respiratory water loss (both p < 0.001). Significant associations with total energy intake were observed for water from food and fecal water loss (both p < 0.01), whereas other water intake components showed no significant associations. Conclusions: In young adults, energy metabolism appears to be more closely associated with physiologically regulated components of water turnover than with voluntary water intake. These findings suggest a divergence between endogenous and behaviorally regulated pathways of water turnover and highlight the importance of considering component-specific water dynamics when examining hydration and energy balance, although confirmation in larger studies is warranted. Full article

5-Hydroxymethylfurfural (5-HMF) and the mycotoxin patulin (PAT) serve as crucial chemical markers for evaluating the quality and safety of fruit-derived beverages, particularly pomegranate juice. This study aimed to quantify the occurrence of 5-HMF and PAT in commercial reconstituted pomegranate juices and assess the associated dietary exposure risks. A total of 154 commercial samples, collected from a Turkish processing facility during the 2024–2025 production seasons, were analysed using high-performance liquid chromatography with diode-array detection. 5-HMF was detected in 152 samples (98.7%) at concentrations ranging from 1.03 to 10.79 mg/kg, with only two samples (1.3%) exceeding the critical threshold of 10 mg/kg. PAT was found in 57 samples (37.0%), with concentrations between 3.61 and 50.69 µg/kg, and only one sample (0.6%) exceeded the European Union maximum level established for fruit juices. Estimated mean daily intakes for adults and children ranged from 0.374 to 2.362 and 1.139 to 8.546 µg/kg bw/day for 5-HMF, and from 0.001 to 0.006 and 0.002 to 0.021 µg/kg bw/day for PAT, respectively. Risk characterisation based on hazard quotient values indicated that PAT exposure did not pose a significant health risk for either population group, highlighting the overall safety of the analysed products. Full article

3-Monochloropropanediol esters (3-MCPDEs) and glycidyl esters (GEs) are food processing contaminants that raise significant food safety concerns due to their established potential for carcinogenicity. This study aimed to determine the occurrence of 3-MCPDEs and GEs in common Malaysian food items and to evaluate the associated health risks through dietary exposure assessment. A total of 251 samples, consisting of retail products and cooked/prepared meals, were analysed using GC-MS. The food consumption data were obtained from published national food surveys. Risk was characterised using health-based guidance values (HBGVs) and margin of exposure (MOE), lifetime cancer risk (LCR), and disability-adjusted life year (DALY) estimates. 3-MCPDE was detected in 94.8% of samples (range: ND to 7.77 mg/kg), while GE was found in 83.3% of samples (range: ND to 9.41 mg/kg). The highest levels were consistently observed in refined vegetable fats and oil products, specifically shortening (3-MCPDE: 3.53 [IQR 2.76–5.16] mg/kg; GE: 4.78 [IQR 3.52–6.14] mg/kg) and margarine (3-MCPDE: 2.50 [IQR 1.11–3.59] mg/kg; GE: 3.60 [IQR 1.18–5.26] mg/kg). Exposure assessment identified fried rice as the largest contributor to total daily intake (3-MCPDE: 3.16 μg/kg BW/day; GE: 1.36 μg/kg BW/day). Total exposure to 3-MCPDE exceeded the provisional maximum tolerable daily intake (PMTDI) established by JECFA by 39.5%, indicating a potential health concern. Low MOE estimates (<10,000) for 3-MCPDE and GE were determined for several food categories, including snacks, kuih-muih, and fried cooked dishes. Chronic GE exposure was estimated to cause up to 6.9 (for mean consumers) and 24.9 (for high consumers) cancer cases per year, with total the DALYs quantified at 124.2 years lost per 100,000 of the population. These data represent a worst-case scenario; however, risks could be minimised through continued surveillance, mitigation strategies by relevant authorities regarding food processing, and informed dietary choices. Full article

The continuous accumulation of micro- and nanoplastics in the human living environment and their deposition in various organs of the body have become a global public health concern with the widespread use of plastic products. This review summarizes the main categories of micro- and nanoplastics entering the body through dietary intake and air inhalation, based on human exposure pathways. By integrating existing literature data, this review estimates the daily intake and excretion of micro- and nanoplastics in humans, summarizes evidence regarding their potential deposition patterns in blood cells and hematopoietic-related organs, mainly inferred from animal and in vitro studies, and discusses the possible impacts of such deposition on hematopoietic function. Furthermore, the toxic effects and potential hazards of micro- and nanoplastics on the human hematopoietic system at both cellular and animal levels, along with the underlying molecular mechanisms, are comprehensively reviewed. From the dual perspectives of environmental governance and bodily protection, exploratory research ideas are proposed, including biodegradation strategies and the application of medicinal and edible homologous substances. This review aims to provide insights for reducing the risk of hematopoietic system diseases and preventing harm caused by micro- and nanoplastics to the human body in the future. Full article