Search Results (13)

Plant mitochondrial genomes are characterized by their complex compositions and structures, large genomes, rapid recombination and evolution rates, and frequent intracellular gene transfer events. Centipedegrass, known as “Chinese turfgrass”, is a warm-season turfgrass that exhibits excellent tolerance to both biotic and abiotic stresses. The chloroplast genome, with 139,107 bp, and the mitochondrial genome, with 564,432 bp, were both assembled into a single circular structure. We identified 44 gene transfer events between the chloroplast and mitochondrial genomes. The mitochondrial gene cox1 could serve as a marker for distinguishing accessions found at different altitudes. The unique features of the centipedegrass mitochondrial genome, coupled with the comparative genomic analysis of both chloroplast and mitochondrial genomes, have the potential to enrich the Poaceae database and provide crucial perspectives on plant evolution, energy metabolism, and responses to environmental conditions. The markers developed could facilitate the analysis of the genetic diversity of centipedegrass. Full article

SQUAMOSA promoter-binding protein-like (SPL) transcription factors play a critical role in the regulation of gene expression and are indispensable in orchestrating plant growth and development while also improving resistance to environmental stressors. Although it has been identified across a wide array of plant species, there have been no comprehensive studies on the SPL gene family in centipedegrass [Eremochloa ophiuroides (Munro) Hack.], which is an important warm-season perennial C4 turfgrass. In this study, 19 potential EoSPL genes in centipedegrass were identified and assigned the names EoSPL1-EoSPL19. Gene structure and motif analysis demonstrated that there was relative consistency among the branches of the phylogenetic tree. Five pairs of segmental duplication events were detected within centipedegrass. Ten EoSPL genes were predicted to be targeted by miR156. Additionally, the EoSPL genes were found to be predominantly expressed in leaves and demonstrated diverse responses to abiotic stress (salt, drought, glufosinate ammonium, aluminum, and cold). This study offers a comprehensive insight into the SPL gene family in centipedegrass, creating a foundation for elucidating the functions of EoSPL genes and investigating their involvement in abiotic stress responses. Full article

Drought stress is a critical abiotic factor that impedes plant growth and development, particularly in arid and semi-arid regions during summer. This study investigated the physiological mechanism of drought tolerance and post-drought recovery in two genotypes of centipedegrass (Eremochloa ophiuroides): the drought-resistant CG101 and the drought-sensitive CG021. The research studied the impacts of drought and subsequent rehydration on turf quality, leaf relative water content, electrolyte leakage rate (EL), photochemical efficiency (F_v/F_m), relative water loss rate (RWLR), and relative water uptake rate as well as the contents of proline, total soluble sugars, betaine, and leaf wax content (LWC) and the morphology of the root system. The findings revealed that the higher drought tolerance of CG101 was primarily associated with the superior cell membrane stability (lower EL), greater F_v/F_m, better water retention capacity (higher LWC and lower RWLR). In addition, the extensive root system of CG101, characterized by greater total root length and surface area, collectively contributed to the stronger drought tolerance of the drought-tolerant CG101 in comparison with the drought-sensitive CG021. During rehydration, the RWC of CG021 did not fully recover to the control levels mainly due to the reduced leaf F_v/F_m, LWC as well as the root length, root surface area, root volume, and the relatively weaker osmotic regulatory ability. This study provides insights into the physiological mechanisms resulting in interspecific variations in drought tolerance and post-drought recovery in centipedegrass, and offers theoretical support for breeding drought-resistant varieties in centipedegrass. Full article

Centipedegrass (Eremochloa ophiuroides) is an important warm-season grass plant used as a turfgrass as well as pasture grass in tropical and subtropical regions, with wide application in land surface greening and soil conservation in South China and southern United States. In this study, the complete cp genome of E. ophiuroides was assembled using high-throughput Illumina sequencing technology. The circle pseudomolecule for E. ophiuroides cp genome is 139,107 bp in length, with a quadripartite structure consisting of a large single copyregion of 82,081 bp and a small single copy region of 12,566 bp separated by a pair of inverted repeat regions of 22,230 bp each. The overall A + T content of the whole genome is 61.60%, showing an asymmetric nucleotide composition. The genome encodes a total of 131 gene species, composed of 20 duplicated genes within the IR regions and 111 unique genes comprising 77 protein-coding genes, 30 transfer RNA genes, and 4 ribosome RNA genes. The complete cp genome sequence contains 51 long repeats and 197 simple sequence repeats, and a high degree of collinearity among E. ophiuroide and other Gramineae plants was disclosed. Phylogenetic analysis showed E. ophiuroides, together with the other two Eremochloa species, is closely related to Mnesithea helferi within the subtribe Rottboelliinae. These findings will be beneficial for the classification and identification of the Eremochloa taxa, phylogenetic resolution, novel gene discovery, and functional genomic studies for the genus Eremochloa. Full article

The TIFY family is a group of novel plant-specific transcription factors involved in plant development, signal transduction, and responses to stress and hormones. TIFY genes have been found and functionally characterized in a number of plant species. However, there is no information about this family in warm-season grass plants. The current study identified 24 TIFY genes in Eremochloa ophiuroides, a well-known perennial warm-season grass species with a high tolerance to aluminum toxicity and good adaptability to the barren acidic soils. All of the 24 EoTIFYs were unevenly located on six out of nine chromosomes and could be classified into two subfamilies (ZIM/ZML and JAZ), consisting of 3 and 21 genes, respectively, with the JAZ subfamily being further divided into five subgroups (JAZ I to JAZ V). The amino acids of 24 EoTIFYs showed apparent differences between the two subfamilies based on the analysis of gene structures and conserved motifs. MCScanX analysis revealed the tandem duplication and segmental duplication of several EoTIFY genes occurred during E. ophiuroides genome evolution. Syntenic analyses of TIFY genes between E. ophiuroides and other five plant species (including A. thaliana, O. sativa, B. distachyon, S. biocolor, and S. italica) provided valuable clues for understanding the potential evolution of the EoTIFY family. qRT-PCR analysis revealed that EoTIFY genes exhibited different spatial expression patterns in different tissues. In addition, the expressions of EoTIFY genes were highly induced by MeJA and all of the EoTIFY family members except for EoJAZ2 displayed upregulated expression by MeJA. Ten EoTIFY genes (EoZML1, EoZML1, EoJAZ1, EoJAZ3, EoJAZ5, EoJAZ6, EoJAZ8, EoJAZ9, EoJAZ10, and EoJAZ21) were observed to be highly expressed under both exogenous MeJA treatment and aluminum stress, respectively. These results suggest that EoTIFY genes play a role in the JA-regulated pathway of plant growth and aluminum resistance as well. The results of this study laid a foundation for further understanding the function of TIFY genes in E. ophiuroides, and provided useful information for future aluminum tolerance related breeding and gene function research in warm-season grass plants. Full article

Centipedegrass (Eremochloa ophiuroides (Munro.) Hack.) is a species originating in China and is an excellent warm-season turfgrass. As a native species in southern China, it is naturally distributed in the phosphorus-deficient and aluminum-toxic acid soil areas. It is important to research the molecular mechanism of centipedegrass responses to phosphorus-deficiency and/or aluminum-toxicity stress. Quantitative Real-Time PCR (qRT-PCR) is a common method for gene expression analysis, and the accuracy of qRT-PCR results depends heavily on the stability of internal reference genes. However, there are still no reported stable and effective reference genes for qRT-PCR analysis of target genes under the acid-soil-related stresses in different organs of centipedegrass. For scientific rigor, the gene used as a reference for any plant species and/or any stress conditions should be first systematically screened and evaluated. This study is the first to provide a group of reliable reference genes to quantify the expression levels of functional genes of Eremochloa ophiuroides under multiple stresses of P deficiency and/or aluminum toxicity. In this study, centipedegrass seedlings of the acid-soil-resistant strain ‘E041’ and acid-soil-sensitive strain ‘E089’ were used for qRT-PCR analysis. A total of 11 candidate reference genes (ACT, TUB, GAPDH, TIP41, CACS, HNR, EP, EF1α, EIF4α, PP2A and actin) were detected by qRT-PCR technology, and the stability of candidate genes was evaluated with the combination of four internal stability analysis software programs. The candidate reference genes exhibited differential stability of expression in roots, stems and leaves under phosphorus-deficiency and/or aluminum-toxicity stress. On the whole, the results showed that GAPDH, TIP41 and HNR were the most stable in the total of samples. In addition, for different tissues under various stresses, the selected reference genes were also different. CACS and PP2A were identified as two stable reference genes in roots through all three stress treatments (phosphate deficiency, aluminum toxicity, and the multiple stress treatment of aluminum toxicity and phosphate deficiency). Moreover, CACS was also stable as a reference gene in roots under each treatment (phosphate deficiency, aluminum toxicity, or multiple stresses of aluminum toxicity and phosphate deficiency). In stems under all three stress treatments, GAPDH and EIF4α were the most stable reference genes; for leaves, PP2A and TIP41 showed the two highest rankings in all three stress treatments. Finally, qRT-PCR analysis of the expression patterns of the target gene ALMT1 was performed to verify the selected reference genes. The application of the reference genes identified as internal controls for qRT-PCR analysis will enable accurate analysis of the target gene expression levels and expression patterns in centipedegrass under acid-soil-related stresses. Full article

As a C4 warm-season turfgrass, centipedegrass (Eremochloa ophiuroides (Munro) Hack.) is known for its exceptional resilience to intensive maintenance practices. In this research, the most stably expressed reference genes in the leaves of centipedegrass under different stress treatments, including salt, cold, drought, aluminum (Al), and herbicide, were screened by the quantitative real-time PCR (RT-qPCR) technique. The stability of 13 candidate reference genes was evaluated by software GeNorm V3.4, NormFinder V20, BestKeeper V1.0, and ReFinder V1.0. The results of this experiment demonstrated that the expression of the UBC (ubiquitin-conjugating enzyme) remained the most stable under cold and Al stress conditions. On the other hand, the MD (malate dehydrogenase) gene exhibited the best performance in leaf tissues subjected to salt and drought stresses. Under herbicide stress, the expression level of the RIP (60S ribosomal protein L2) gene ranked the highest. The expression levels of abiotic stress-associated genes such as PIP1, PAL, COR413, ALMT9, and BAR were assessed to validate the reliability of the selected reference genes. This study provides valuable information and reference points for gene expression under abiotic stress conditions in centipedegrass. Full article

Biological nitrification inhibition (BNI) in the tropical grass Brachiaria humidicola could reduce net nitrification rates and nitrous oxide (N₂O) emissions in soil. To determine the effect on gross nitrogen (N) transformation processes and N₂O emissions, an incubation experiment was carried out using ¹⁵N tracing of soil samples collected following 2 years of cultivation with high-BNI Brachiaria and native non-BNI grass Eremochloa ophiuroide. Brachiaria enhanced the soil ammonium (NH₄⁺) supply by increasing gross mineralization of recalcitrant organic N and the net release of soil-adsorbed NH₄⁺, while reducing the NH₄⁺ immobilization rate. Compared with Eremochloa, Brachiaria decreased soil gross nitrification by 37.5% and N₂O production via autotrophic nitrification by 14.7%. In contrast, Brachiaria cultivation significantly increased soil N₂O emissions from 90.42 μg N₂O-N kg⁻¹ under Eremochloa cultivation to 144.31 μg N₂O-N kg⁻¹ during the 16-day incubation (p < 0.05). This was primarily due to a 59.6% increase in N₂O production during denitrification via enhanced soil organic C, notably labile organic C, which exceeded the mitigated N₂O production rate during nitrification. The contribution of denitrification to emitted N₂O also increased from 9.7% under Eremochloa cultivation to 47.1% in the Brachiaria soil. These findings confirmed that Brachiaria reduces soil gross nitrification and N₂O production via autotrophic nitrification while efficiently stimulating denitrification, thereby increasing soil N₂O emissions. Full article

Tillering is a key factor that determines the reproductive yields of centipedegrass, which is an important perennial warm-season turfgrass. However, the regulatory mechanism of tillering in perennial plants is poorly understood, especially in perennial turfgrasses. In this study, we created and characterised a cold plasma-mutagenised centipedegrass mutant, mtn1 (more tillering number 1). Phenotypic analysis showed that the mtn1 mutant exhibited high tillering, short internodes, long seeds and a heavy 1000-seed weight. Then, a comparative transcriptomic analysis of the mtn1 mutant and wild-type was performed to explore the molecular mechanisms of centipedegrass tillering. The results revealed that plant hormone signalling pathways, as well as starch and sucrose metabolism, might play important roles in centipedegrass tillering. Hormone and soluble sugar content measurements and exogenous treatment results validated that plant hormones and sugars play important roles in centipedegrass tiller development. In particular, the overexpression of the auxin transporter ATP-binding cassette B 11 (EoABCB11) in Arabidopsis resulted in more branches. Single nucleotide polymorphisms (SNPs) were also identified, which will provide a useful resource for molecular marker-assisted breeding in centipedegrass. According to the physiological characteristics and transcriptional expression levels of the related genes, the regulatory mechanism of centipedegrass tillering was systematically revealed. This research provides a new breeding resource for further studies into the molecular mechanism that regulates tillering in perennial plants and for breeding high-tillering centipedegrass varieties. Full article

In southern Taiwan, mudstone badland accounts for over 1000 km² of the upstream region of watersheds. Rainstorms often induce interrill and surface erosion on the mudstone slopes. Furthermore, the large quantity of soils detached by surface runoff result in severe sedimentation in reservoirs. Thus, soil erosion control of mudstone badlands represents one of the most pressing problems in reservoir watershed management. Cynodon dactylon (L.) Pers. (Bermuda grass) and Eremochloa ophiuroides (Munro) Hack. (Centipedegrass) are two native predominant C₄ grass species appearing on mudstone badlands. They play a key role in erosion control and the revegetation of mudstone slopes. Nevertheless, their root functional traits and water erosion-reducing potential have not been investigated. In this study, the root traits were examined. Vertical pullout and tensile tests were conducted to measure root pullout resistance and root tensile strength. Hydraulic flume tests were also performed to evaluate their water erosion-reducing potentials. The results demonstrated that the root systems of C. dactylon and E. ophiuroides grasses all belonged to the fibrous M-type. C. dactylon had remarkably better root traits compared to those of E. ophiuroides. Furthermore, the root tensile resistance of C. dactylon was remarkably higher than that of E. ophiuroides. In addition, hydraulic flume tests showed that C. dactylon has remarkably smaller soil detachment rates than that of E. ophiuroides. Altogether, our data clearly show that C. dactylon has better root traits, root pullout resistance, root tensile resistance and water erosion-reducing potential than E. ophiuroides and is more suitable for erosion control of mudstone badland. Further studies on large-scale implementation techniques of these species for efficient vegetation restoration are needed. Full article

Turfgrasses are commonly used for lawns and as recreational surfaces in the USA. Because grasses are largely wind-pollinated, it was thought that pollinators would not forage on turfgrasses. Centipede grass (Eremochloa ophiuroides (Munro) Hack) is a warm-season turfgrass widely used in the southeastern USA. Centipede grass produces spike-like inflorescences from August to October, and little is known about whether pollinators utilize those inflorescences as pollen resources. Thus, the objective of the current study was to identify the pollinators foraging on centipede grass inflorescences. Pollinator samples were collected by (1) sweeping the insects actively foraging on centipede grass inflorescence for 30 min, (2) deploying pan traps for 24 h and (3) deploying malaise traps for 7 d. In the sweep samples, Lasioglossum spp., Bombus spp., Apis spp., Melissodes spp. and Augochlorella spp. were collected from centipede grass inflorescences. Syrphid flies were also collected in the sweep samples. The pan and malaise traps collected mostly Lasioglossum spp. The results imply that there is a critical need to conserve bee habitats and adopt nondisruptive lawn practices. Additionally, this new knowledge lays the foundation for future research to enhance our understanding of bee and syrphid behavior and the selection of host traits for improving bee foraging. Full article

Centipedegrass (Eremochloa ophiuroides) is a low-maintenance turfgrass. The first extensive green roof of centipedegrass was established in TongZhou Civil Squares in 2014. However, storm-water-runoff reduction, water-retention capacity, and plant-water requirements by a centipedegrass green roof has not yet been defined. The soil moisture dynamics, rainwater-retention capacity, runoff reduction, and plant evapotranspiration were investigated by simulated centipedegrass green roof plots, which were constructed in the same manner as the green roofs in TongZhou Civil Squares in 2018. The results showed that the centipedegrass green roof retained 705.54 mm of rainwater, which consisted 47.4% of runoff reduction. The saturated soil moisture was 33.4 ± 0.6%; the excess rainfall over the saturated soil moisture resulted in runoff. The capacity of rainwater retention was negatively related to the soil moisture before rain events and was driven by plant evapotranspiration. Drought symptoms only occurred three times over the course of a year when the soil moisture dropped down to 10.97%. Our results indicate that the rainwater retained in the soil almost met the needs of plant consumption; a further increase of rainwater retention capacity might achieve an irrigation-free design in a centipedegrass green roof. Full article

Activity-guided fractionation of a methanolic extract of the leaves of Eremochloa ophiuroides (centipede grass) using a pancreatic lipase inhibitory assay led to the isolation and identification of a new C-glycosidic flavone, luteolin 6-C-b-D-boivinopyranoside (1), as well as eight known compounds. The structures of these compounds were established on the basis of interpretation of their spectroscopic data. Among these isolates, the C-glycosidic flavones 1–5 showed potent inhibitory effects on pancreatic lipase, with IC₅₀ values ranging from 18.5 ± 2.6 to 50.5 ± 3.9 mM. Full article