Search Results (513)

Late Pleistocene coastal deposits on the southeastern coast of Formentera (Es Ram–Es Estufadors) provide a high-resolution record of sea-level and climatic fluctuations associated with Marine Isotope Stage (MIS) 5. Three distinct beach levels (Sef-1, Sef-2, Sef-3) were identified, corresponding to substages MIS 5e, 5c, and possibly 5a, based on sedimentological features, fossil assemblages, and Optically Stimulated Luminescence (OSL) dating. The oldest beach level (Sef-1) is attributed to MIS 5e (ca. 128–116 ka) and is characterised by the widespread presence of thermophilic Senegalese fauna—including Thetystrombus latus, Conus ermineus, and Linatella caudata—which mark the onset of this interglacial phase and are associated with two peaks in relative sea-level highstand. A subsequent cooling event during MIS 5d is recorded by the development of thin palaeosols and the disappearance of these warm-water taxa. The second beach level (Sef-2) reflects renewed sea-level rise and warmer conditions during MIS 5c, with abundant macrofauna and red algae. The transition to MIS 5b (~97 ka) is marked by a significant sea-level drop (down to –60 m), cooler climate, and enhanced colluvial sedimentation linked to increased runoff and erosion. In total, 54 macrofaunal species were identified—16 from Sef-1 and 46 from Sef-2—highlighting ecological shifts across substages. These results improve our understanding of coastal response to sea-level oscillations and paleoenvironmental dynamics in the western Mediterranean during the Late Pleistocene. Full article

This paper compares two methods, inferential statistics and Systems Dynamics, to study the evolution of individual happiness after a single dose of drug consumption. In an application case, the effect of alcohol and caffeine on happiness is analyzed through a single-case experimental design, with replication, involving two participants. Both inferential statistical analysis and Systems Dynamics methods have been used to analyze the results. Two scales were used to measure happiness—the Euphoria Scale (ES) and the Smiling Face Scale (SFS)—in trait and state format. A single-case experimental ABC design was used. Phase A had no treatment, and Phases B and C saw both subjects receiving 26.51 mL of alcohol and 330 mg of caffeine, respectively. The participants filled in a form with both scales in a state format every 10 min over a 3 h period, operating in each one of the three phases A, B and C. The main conclusion of the analysis performed is that both methods provide similar results about the evolution of individual happiness after single dose consumption. Therefore, the article shows that inferential statistics and the stimulus response model derived from the Systems Dynamics approach can be used in a complementary and enriching way to obtain prediction results. Full article

Background/Objectives: Cooling strategies are critical for optimizing athlete recovery and performance in team sports, yet practical, accessible solutions remain underexplored. This study evaluated the efficacy of a cool vest during a 15 min recovery period following 45 min of simulated soccer match play on core and skin temperature regulation. Methods: Forty-seven physically active males completed an exercise protocol replicating the physiological demands of a soccer half-match. Participants were randomized into an experimental group using a cool vest during recovery (IG, n = 24) or a control group (CG, n = 23) without cooling intervention. Results: Compared to CG, IG exhibited small but significant reductions in skin temperature (31.46 ± 0.67 °C vs. 32.40 ± 1.04 °C; ES = −0.24, 95% CI: −0.40 to −0.08; p = 0.003) and tympanic temperature (35.99 ± 0.45 °C vs. 36.54 ± 0.66 °C; ES = −0.43, 95% CI: −0.67 to −0.18; p < 0.001) 10 min post-exercise. These differences intensified to small and moderate effects at 15 min post-exercise for skin (31.07 ± 0.67 °C vs. 32.51 ± 0.91 °C; ES = −0.37, 95% CI: −0.53 to −0.21; p < 0.001) and tympanic temperatures (35.70 ± 0.42 °C vs. 36.65 ± 0.58 °C; ES = −0.71, 95% CI: −0.96 to −0.46; p < 0.001), respectively, with IG maintaining consistently lower values. No temperature changes were observed in CG. Conclusions: These findings demonstrate that a 15 min cool vest application during halftime effectively reduces core and skin temperatures, compared to passive recovery. This supports its utility as a practical, low-cost strategy for thermoregulatory management in soccer, warranting further investigation into its long-term performance benefits. Full article

The use of astaxanthin (AST) is expanding from its origins as a food coloring to health-related applications. This paper evaluates the efficiency of its extraction from dried Xanthophyllomyces dendrorhous using two combined and consecutive techniques. First, cell membrane permeation is achieved with the application of pulsed electric fields (PEFs). Solid–liquid extraction is then performed with hydrophobic eutectic solvents (hESs) prepared by mixing components of essential oils (linalool, l-menthol, eugenol, geraniol, cinnamyl alcohol, or thymol) and oleic acid. The hESs were characterized by measuring of several thermophysical properties at 25 °C and 0.1 MPa. An initial screening was performed to choose the best solvent and the extraction conditions (composition, extraction time, and temperature) were evaluated using the response surface methodology. The results showed the importance of the electroporation as a preliminary step to the extraction. The more hydrophobic and less compact the hES, the more effective the solvent. Thus, the equimolar mixture of l-menthol and oleic acid achieved an efficiency of 77% for untreated biomass, 83% for that treated with PEF, and 92% for that treated with PEF and later incubated. Molecular dynamics simulations demonstrated the importance of the hydrophobic interactions between AST and the components of the best solvent. Full article

ES5-4^T, a Gram-positive, motile, aerobic, and rod-shaped strain, was isolated from the rhizosphere of Galinsoga parviflora growing in the selenium-rich ore area of Enshi, Hubei Province, China. This strain can grow at pH levels of 5.0–10.0 and temperatures of 4–42 °C, with optimal growth at pH 7.0 and 28 °C. It was found to resist NaCl up to 5% (w/v), with an optimal growth condition of 0.5–1.0%. The strain exhibited tolerance to selenite (Se⁴⁺) concentrations up to 5000 mg/L. The major fatty acids of the ES5-4^T strain were anteiso-C_15:0 (46.5%) and C_16:0 (21.7%), its predominant respiratory quinone was MK-7, and its polar lipids included diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and an unidentified phospholipid (PL). The presence of the 16S rRNA gene sequence implies that ES5-4^T belongs to a member of the genus Paenibacillus, with the highest sequence similarity of 98.4% to Paenibacillus pabuli NBRC 13638^T. The bac120 tree also confirmed that the strain is within the genus Paenibacillus. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between ES5-4^T and closely related members of the genus Paenibacillus were all below the cutoff levels of 95–96% and 70%, respectively. Based on a polyphasic approach, including phenotypic, chemotaxonomic, and phylogenetic analyses, the ES5-4^T strain is proposed as a novel species of the genus Paenibacillus, for which the name Paenibacillus hubeiensis sp. nov. is proposed. This type strain is designated as ES5-4^T (=GDMCC 1.3540^T = KCTC 43478^T). Full article

The occurrence of construction site fires is consistently accompanied by casualties and property damage. To address the issues of large target-scale variations and frequent false detections in construction site fire monitoring, we propose a fire detection algorithm based on an improved YOLOv8 model, achieving real-time and efficient detection of fires on construction sites. First, considering the wide range of scale variations in detected objects, an additional detection layer with a 64-times down-sampling rate is introduced to enhance the algorithm’s detection capability for multi-scale targets. Then, the MBConv module and the ESE attention block are integrated into the C2f structure to enhance feature extraction capabilities while reducing computational complexity. An iCBAM attention module is designed to suppress background noise interference and enhance the representation capability of the network. Finally, the WIoUv3 metric is adopted in the loss function for bounding box regression to mitigate harmful gradient issues. Comparative experiments demonstrate that, on a self-constructed construction site fire dataset, the improved algorithm achieves an accuracy and recall increase of 4.6% and 3.0%, respectively, compared to the original YOLOv8 model. Additionally, mAP50 and mAP50-95 are improved by 1.6% and 1.5%, respectively. This algorithm provides a more effective solution for fire monitoring in construction environments. Full article

The massive heavy oil reserves in the Athabasca region of northern Alberta depend on steam-assisted gravity drainage (SAGD) for their economic exploitation. Even though SAGD has been successful in highly viscous oil recovery, it is still a costly technology because of the large energy input requirement. Large water and natural gas quantities needed for steam generation imply sizable greenhouse gas (GHG) emissions and extensive post-production water treatment. Several methods to make SAGD more energy-efficient and environmentally sustainable have been attempted. Their main goal is to reduce steam consumption whilst maintaining favourable oil production rates and ultimate oil recovery. Oil saturation within the steam chamber plays a critical role in determining both the economic viability and resource efficiency of SAGD operations. However, accurately quantifying the residual oil saturation left behind by SAGD remains a challenge. In this experimental research, sand pack Expanding Solvent SAGD (ES-SAGD) coinjection experiments are reported in which Pentane -C₅H₁₂, and Hexane -C₆H₁₄ were utilised as an additive to steam to produce Long Lake bitumen. Each solvent is assessed at three different constant concentrations through time using experiments simulating SAGD to quantify their impact. The benefits of single-component solvent coinjection gradually diminish as the SAGD process approaches its later stages. ES-SAGD pentane coinjection offers a smaller improvement in recovery factor (RF) (4% approx.) compared to hexane (8% approx.). Between these two single-component solvents, 15 vol% hexane offered the fastest recovery. The obtained data in this research provided compelling evidence that the coinjection of solvent under carefully controlled operating conditions, reduced overall steam requirement, energy consumption, and residual oil saturation allowing proper adjustment of oil and water relative permeability curve endpoints for field pilot reservoir simulations. Full article

Background: Endocrine therapies are accompanied by side effects that significantly impact the quality of life (QoL) of women with breast cancer. Adequate diet is important for fulfilling nutritional requirements, preserving health, and supporting therapy in this vulnerable population. Methods: This preliminary study evaluated the QoL of life and dietary intake in 185 women with breast cancer on two therapies, aromatase inhibitors (AIs) and tamoxifen, using the Functional Assessment of Cancer Therapy—Endocrine Symptoms (FACT-ES), European Organization for Research and Treatment of Cancer Quality of Life Questionnaire (EORTC QLQ-C30), and Breast Cancer Specific Questionnaire (QLQ-BR23) and a 24 h dietary recall. A total of 185 women were included in the study and fulfilled the FACT-ES, of whom 73 fulfilled other two questionnaires and a 24 h recall. Results: No significant differences were found in the overall QoL between groups. Joint pain (95.3%) and reduced libido (84.7%) were most common with AIs, while tamoxifen users more frequently reported weight gain and irritability (93.0%, each), and vasomotor and gynecological symptoms. Macronutrient intake was similar, though AIs users consumed more energy-dense (p ≤ 0.001) and sugary foods (p = 0.034), while tamoxifen users had higher omega-6 PUFA intake. Both groups exhibited suboptimal intake of vitamin D, calcium, and selenium, and a higher phosphorus consumption relative to recommended daily values. Conclusions: Preliminary findings showed that QoL and dietary intake were comparable between patients with BC on AIs and tamoxifen treatment. Endocrine-related symptoms were more prevalent among tamoxifen users, whereas joint pain was most common in AIs users. Nutritional interventions may be warranted in both groups to ensure adequate intake of essential micronutrients in accordance with recommended dietary guidelines. Full article

This study proposes an RBF-PSO hybrid framework for efficient inversion analysis of hydration heat parameters in mass concrete temperature fields, addressing the computational inefficiency and accuracy limitations of traditional methods. By integrating a Radial Basis Function (RBF) surrogate model with Particle Swarm Optimization (PSO), the method reduces reliance on costly finite element simulations while maintaining global search capabilities. Three objective functions—integral-type (F₁), feature-driven (F₂), and hybrid (F₃)—were systematically compared using experimental data from a C40 concrete specimen under controlled curing. The hybrid F₃, incorporating Dynamic Time Warping (DTW) for elastic time alignment and feature penalties for engineering-critical metrics, achieved superior performance with a 74% reduction in the prediction error (mean MAE = 1.0 °C) and <2% parameter identification errors, resolving the phase mismatches inherent in F₂ and avoiding F₁’s prohibitive computational costs (498 FEM calls). Comparative benchmarking against non-surrogate optimizers (PSO, CMA-ES) confirmed a 2.8–4.6× acceleration while maintaining accuracy. Sensitivity analysis identified the ultimate adiabatic temperature rise as the dominant parameter (78% variance contribution), followed by synergistic interactions between hydration rate parameters, and indirect coupling effects of boundary correction coefficients. These findings guided a phased optimization strategy, as follows: prioritizing high-precision calibration of dominant parameters while relaxing constraints on low-sensitivity variables, thereby balancing accuracy and computational efficiency. The framework establishes a closed-loop “monitoring-simulation-optimization” system, enabling real-time temperature prediction and dynamic curing strategy adjustments for heat stress mitigation. Robustness analysis under simulated sensor noise (σ ≤ 2.0 °C) validated operational reliability in field conditions. Validated through multi-sensor field data, this work advances computational intelligence applications in thermomechanical systems, offering a robust paradigm for parameter inversion in large-scale concrete structures and multi-physics coupling problems. Full article

Soil-transmitted helminths (STHs) are parasitic nematodes that infect humans, particularly in tropical and subtropical regions, where they contribute substantially to neglected tropical diseases (NTDs). Among them, hookworms (Ancylostoma duodenale, Necator americanus and Ancylostoma ceylanicum) cause substantial morbidity, leading to anaemia, malnutrition, and developmental impairment. Despite the global impact of hookworm disease, genomic research on A. duodenale has lagged behind that of other hookworms, limiting comparative and molecular biological investigations. Here, we report the first chromosome-level reference genome of A. duodenale, assembled from a single adult specimen archived in ethanol at −20 °C for more than 27 years. Using third-generation sequencing (PacBio Revio, Menlo Park, CA, USA, Oxford Nanopore, Oxford, UK), Hi-C scaffolding, and advanced computational tools, we produced a high-quality 319 Mb genome, filling a critical gap in hookworm genomics. Comparative analyses with N. americanus and the related, free-living nematode Caenorhabditis elegans provided new insights into genome organisation, synteny, and specific adaptations. While A. duodenale exhibited strong chromosomal synteny with N. americanus, its limited synteny with C. elegans highlights its distinct parasitic adaptations. We identified 20,015 protein-coding genes, including conserved single-copy orthologues (SCOs) linked to host–pathogen interactions, immune evasion and essential biological processes. The first comprehensive secretome analysis of A. duodenale revealed a diverse repertoire of excretory/secretory (ES) proteins, including immunomodulatory candidates predicted to interact with host structural and immune-related proteins. This study advances hookworm genomics, establishes a basis for the sequencing of archival specimens, and provides fundamental insights into the molecular biology of A. duodenale. The genomic resource for this hookworm species creates new opportunities for diagnostic, therapeutic, and vaccine development within a One Health framework. It complements recent epidemiological work and aligns with the WHO NTD roadmap (2021–2030) and Sustainable Development Goal 3.3. Full article

The most investigated ABCB1 single-nucleotide polymorphisms (SNPs) related to antiseizure medication resistance are rs1045642 (c.3435C>T, p.Ile1145=), rs2032582 (c.2677G>T/A, p.Ala893Ser/Thr), and rs1128503 (c.1236C>T, p.Gly412=). We conducted a literature review to evaluate the genotype frequencies of rs1045642, rs2032582, and rs1128503 SNPs in different ancestries among the drug-resistant and drug-responsive epilepsy groups. Furthermore, we performed effect size and study power analyses and determined the expected sample size to reach a study power of 0.8 for each conducted research. High and very high statistical power for the rs1045642, rs2032582, and rs1128503 polymorphisms was achieved in 58.0, 60.7, and 31.8% of the studies, respectively. The effect sizes (ES) of rs1045642, rs2032582, and rs1128503 ranged from 0.03–1.04, 0.06–0.92, and 0.04–0.64, respectively. The required sample sizes for rs1045642, rs2032582, and rs1128503 ranged from 9–13,000, 12–2600, and 24–5700 participants, respectively. None of the polymorphisms showed a statistically significant association with antiseizure medication resistance in the forest plots. Our analysis provides valuable guidance for future genetic association studies in the field of drug-resistant epilepsy. Full article

Exploring advanced sulfur cathode materials is important for the development of lithium-sulfur batteries (LSBs), but they still present challenges. Herein, zinc oxide dots-modified vanadium nitride flower-like heterojunctions (Zn-QDs-VN) as sulfur hosts are prepared by a phase separation strategy. Characterizations confirm that the flower structure with high specific surface area and pores improves active site exposure and electron/mass transfer. In situ phase separation enriches the Zn-QDs-VN interface, addressing the issues of uneven distribution and interface reduction of Zn-QDs-VN. Further theoretical computations reveal that ZnO-QDs-VN with optimized intermediate spin states can constitute a stable LiS* bond sequence, which can conspicuously facilitate the adsorption and conversion of LiPSs and reduce the battery reaction energy barrier. Therefore, the ZnO-QDs-VN@S cathode shows a high initial specific capacity of 1109.6 mAh g⁻¹ at 1.0 C and long cycle stability (maintaining 984.2 mAh g⁻¹ after 500 cycles). Under high S loading (8.5 mg cm⁻²) and lean electrolyte conditions (E/S = 6.5 μL mg⁻¹), it also exhibits a high initial area capacity (10.26 mAh cm⁻²) at 0.2 C. The interfacial synergistic effect accelerates the adsorption and conversion of LiPSs and reduces the energy barriers in cell reactions. The study provides a new method for designing heterojunctions to achieve high-performance LSBs. Full article

Electrical stimulation (ES) is widely employed in both clinical therapies and research settings where it has shown promise in promoting tissue regeneration, wound healing, and inflammation control. Research has also highlighted ES as a regulator of DNA demethylation, which plays a critical role in nerve regeneration and cellular repair mechanisms. While the impact of ES on epigenetic processes is recognized, its broader effects on cellular functions, particularly in inflammation and wound healing, are less understood. We recently showed how ES impacts inflammatory states by modulating transcriptomic and metabolomic profiles in a 3Din vitromodel where human fibroblasts and keratinocytes are included in a collagen matrix, i.e., even in the absence of the nervous system. Here, we propose to deepen our exploration on the differential effects on DNA methylation, including an investigation of the correlation with age acceleration using a mitotic clock. These results confirm and caution on the differential effect of DC on inflamed and non-inflamed samples and suggest an involvement of direct current stimuli at 1 V ($DC1$) in the control of senescent processes associated with mitosis and inflammation; the mechanistic details of these will have to be clarified with additional experiments. Full article

Augmented Reality (AR) seamlessly blends the real-world environment with digitally generated content, creating an interactive hybrid experience where both realities coexist. This paper explores an augmented reality application developed for natural heritage education, specifically designed to enhance indoor learning. The focus is on a learning activity titled Exploring the Aquarium, implemented by the Education Centre for the Environment and Sustainability (E.S.E.C.) of Kastoria as part of an environmental education program. The activity enriches students’ knowledge and experiences during their aquarium visit, fosters active participation in the learning process, stimulates cognitive interest, and encourages actions that support the ecological restoration of aquatic ecosystems. This paper presents the application’s design criteria, thematic focus, learning objectives, and core functionalities. Additionally, the paper presents findings from quantitative research evaluating the learning experience. A questionnaire tailored for AR applications was employed to assess aspects such as challenge, educational value (knowledge gained), user collaboration, and intention to reuse the app. Data were collected from 148 K-12 students during the 2023–2024 school year. Descriptive statistical analysis revealed that all factors were evaluated highly. The results indicate that the AR-enhanced educational activity captured the students’ interest and facilitated a collaborative learning environment. The application was positively rated for its functionality, usability, informational content, and the satisfaction it provided, as well as its ability to encourage cooperation and future reuse. Full article

Background/Objectives: Human metapneumovirus (hMPV) is a leading cause of respiratory infections in the elderly, with high morbidity and mortality and with no vaccines or specific therapies available. The primary protective antigen of hMPV is the fusion protein, and its prefusion conformation (pre-F) is considered the most promising target for vaccine development. Methods: Utilizing computational design strategies focused on intraprotomer interface stabilization, we designed hMPV pre-F recombinant subunit vaccine candidates based on the most prevalent A2 subtype and characterized them in vitro and in vivo, benchmarking to the prototypical hMPV pre-F stabilized by an introduction of a proline at site 185. Results: The top candidate (N46V_T160F) yielded 14.4 mg/L with a melting temperature of 79.3 °C as compared to 5.7 mg/L and 70.4 °C for the benchmark. By employing monoclonal antibody binding to all six antigenic sites of hMPV pre-F, we confirmed this construct retained all pre-F specific antigenic sites and that the key sites Ø and V were stable at 4 °C for up to 6 months. When immunogenicity of N46V_T160F was evaluated in mice, it induced higher binding and neutralizing antibody titers than the benchmark, which stemmed in part from increased levels of site Ø and site II targeting Abs. Further, this A2 based construct induced cross-neutralizing Abs against all four hMPV subtypes. Lastly, our construct exhibited similar immunogenicity as the recently published next-generation hMPV pre-F constructs, DS-CavEs2 and v3B_Δ12_D454C-V458C. Conclusions: N46V_T160F is a promising hMPV vaccine candidate paving the way for further development and optimization. Full article