Search Results (14)

Within the scope of “Eco Map of Zagreb” project, eight sensor sets (type AQMeshPod) were set up at an automatic measuring station at the Institute for Medical Research and Occupational Health (IMROH) for comparison with reference methods for air quality measurement during 2018. This station is a city background station within the Zagreb network for air quality monitoring, where measurements of SO₂, CO, NO₂, O₃, PM₁₀ and PM_2.5, are performed using standardized methods accredited according to EN ISO/IEC 17025. This paper presents a comparison of pollutant mass concentrations determined by sensors with reference methods. The data were compared and filtered to remove outliers and handle deviations between the results obtained by sensors and reference methods, considering the different approaches to gas and PM data. A comparison of sensor results with the reference methods showed a large scattering of all gaseous pollutants while the comparison for PM₁₀ and PM_2.5 indicated a satisfactory low dispersion. The results of a regression analysis showed a significant seasonal dependence for all pollutants. Significant statistical differences between the reference methods and sensors for the whole year and in all seasons for all gas pollutants, as well as for PM₁₀, were observed, while for PM_2.5 statistical significance showed varying results. Full article

The adaptation of autochthonous olive varieties to local soil and climatic conditions can lead to a unique chemical composition and characteristics of olive oils that may differ from the generally accepted quality standards set out in the International Olive Oil Council strategy documents and EU regulations. Therefore, the fatty acid composition, biophenol, tocopherol, sterol and triterpenic dialcohol content and composition of the autochthonous Slovenian olive varieties ‘Buga’, ‘Črnica’ and ‘Drobnica’ were studied for a three-year period with the aim of valorising the characteristics of the three olive varieties. Standardised and accredited analytical methods in accordance with SIST EN ISO/IEC 17025:2017 were applied. The results of the investigation showed that the highest average amount of oleic acid (75.75%) was found in the oils of the ‘Črnica’ variety, followed by the ‘Drobnica’ (72.06%) and the ‘Buga’ (68.73%). All three varieties are a good source of total biophenols (‘Buga’ 616 mg/kg, ‘Drobnica’ 569 mg/kg and ‘Črnica’ 427 mg/kg) and α-tocopherol (‘Buga’ 378 mg/kg, ‘Drobnica’ 279 mg/kg, and ‘Črnica’ 243 mg/kg). ‘Buga’ and ‘Drobnica’ are characterised by high amounts of total sterols, 2468 mg/kg and 2391 mg/kg, respectively, while ‘Črnica’ oils, in comparison, showed a lower average value of total sterols (1351 mg/kg). Due to their exceptional chemical composition, ‘Buga’, ‘Črnica’ and ‘Drobnica’ show great potential for the further cultivation and valorisation of traditional olive oil production in the region, thus contributing to the preservation of biodiversity and local traditions. The quality parameters of olive oil from the autochthonous Slovenian olive varieties ‘Buga’, ‘Črnica’ and ‘Drobnica’ also fulfil the limits for extra virgin olive oil according to the Commission Delegated Regulation (EU) 2022/2104, despite local climatic influences. However, accelerated growth due to climatic changes affecting early harvest can lead to them falling outside these limits, which was observed in particular for the ‘Buga’ variety in terms of the linoleic acid content. This study emphasises the importance of timing the harvest to achieve optimum maturity and meet EU quality standards, taking into account the genetic makeup of the varieties and their response to the current climatic conditions. Full article

Paralytic shellfish poisoning is an important concern for mollusk fisheries, aquaculture, and public health. In Galicia, NW Iberian Peninsula, such toxicity has been monitored for a long time using mouse bioassay. Therefore, little information exists about the precise toxin analogues and their possible transformations in diverse mollusk species and environments. After the change in the European PSP reference method, a refinement of the Lawrence method was developed, achieving a 75% reduction in chromatogram run time. Since the beginning of 2021, when this refinement Lawrence method was accredited under the norm UNE-EN ISO/IEC 17025, it has been used in the area to determine the toxin profiles and to estimate PSP toxicity in more than 4500 samples. In this study, we have summarized three years of monitoring results, including interspecific, seasonal, and geographical variability of PSP toxicity and toxin profile. PSP was detected in more than half of the samples analyzed (55%), but only 4.4% of the determinations were above the EU regulatory limit. GTX1,4 was the pair of STX analogs that produced the highest toxicities, but GTX2,3 was found in most samples, mainly due to the reduction of GTX1,4 but also by the higher sensitivity of the method for this pair of analogs. STX seems to be mainly a product of biotransformation from GTX2,3. The studied species (twelve bivalves and one gastropod) accumulated and transformed PSP toxins to a different extent, with most of them showing similar profiles except for Spisula solida and Haliotis tuberculata. Two seasonal peaks of toxicity were found: one in spring-early summer and another in autumn, with slightly different toxin profiles during outbreaks in relation to the toxicity during valleys. In general, both the total toxicity and toxin profiles of the southernmost locations were different from those in the northern part of the Atlantic coast and the Cantabrian Sea, but this general pattern is modified by the PSP history of some specific locations. Full article

African swine fever virus is considered an emerging virus that causes African swine fever, a disease characterised by high mortality and elevated transmission rates and that, as it is for most other viral diseases, cannot be treated with specific drugs. Effective and reliable detection of the virus is relevant to prevent uncontrolled contagion among boar populations and to reduce economic losses. Moreover, animal health laboratories are demanding standardisation, optimisation and quality assurance of the available diagnostic assays. In the present study, the ASFV MONODOSE dtec-qPCR kit was validated following the UNE-EN ISO/IEC 17025:2005 guidelines. Analytical validation terms include in silico and in vitro specificity, sensitivity, efficiency and reliability (repeatability/reproducibility). Diagnostic validation of the method was assessed through the analysis of a total of 181 porcine samples originating from six different matrix types doped with African swine fever virus DNA received from the European reference laboratory for African Swine Fever (INIA-CISA, Madrid, Spain): whole blood, blood serum, kidney, heart, liver and tonsil. Results agreed with those obtained from a reference detection method also based on real-time PCR, endorsed by WOAH, but the ASFV MONODOSE dtec-qPCR kit incorporates some technical innovations and improvements which may benefit end-users. This kit, available worldwide with full analytical and diagnostic validation, can recognise all known ASFV genotypes and brings additional benefits to the current qPCR technology. Full article

Human mpox is caused by the Monkeypox virus, a microorganism closely related to the Variola virus, both belonging to the Orthopoxvirus genus. Mpox had been considered a rare disease until a global outbreak occurred in 2022. People infected with the virus present similar symptoms to patients suffering smallpox and other rash illnesses, hindering diagnosis. The WHO indicated that no commercial PCR or serology kits are currently widely available. In the present study, the MPXV MONODOSE dtec-qPCR kit was validated following guidelines of the UNE/EN ISO/IEC 17025:2005. The parameters evaluated for the acceptance of the assay were in silico and in vitro specificity, quantitative phase analysis, reliability, and sensitivity. The assay passed validation criteria and yielded an efficiency of 95.8%, high repeatability, reproducibility, and a Limit of Detection and Quantification of at least 10 copies. Results from the validation of the MPXV dtec-qPCR kit were satisfactory. The use of the MONODOSE format (dehydrated single PCR-tubes, ready to use) provided considerable advantages allowing the detection of the Monkeypox virus to be accurately achieved. This detection kit may be considered a reliable, fast, simple, and universally available option. Full article

Paralytic Shellfish Toxins (PSTs) are marine biotoxins, primarily produced by dinoflagellates of the genera Gymnodinium spp., Alexandrium spp. They can accumulate in shellfish and, through the food chain, be assimilated by humans, giving rise to Paralytic Shellfish Poisoning. The maximum permitted level for PSTs in bivalves is 800 μg STX·2HCl eqv/kg (Reg. EC N° 853/2004). Until recently, the reference analytical method was the Mouse Bioassay, but Reg. EU N° 1709/2021 entered into force on 13 October 2021 and identified in the Standard EN14526:2017 or in any other internationally recognized validated method not entailing the use of live animals as official methods. Then the official control laboratories had urgently to fulfill the new requests, face out the Mouse Bioassay and implement instrumental analytical methods. The “EURLMB SOP for the analysis of PSTs by pre-column HPLC-FLD according to OMA AOAC 2005.06” also introduced a simplified semiquantitative approach to discriminate samples above and below the regulatory limit. The aim of the present paper is to present a new presence/absence test with a cut-off at 600 μg STX·2HCl eqv/kg enabling the fast discrimination of samples with very low PSTs levels from those to be submitted to the full quantitative confirmatory EN14526:2017 method. The method was implemented, avoiding the use of a large number of certified reference standards and long quantification procedures, resulting in an efficient, economical screening instrument available for official control laboratories. The protocol was fully validated, obtaining good performances in terms of repeatability (<11%) and recovery (53–106%) and accredited according to ISO/IEC 17025. The method was applied to mollusks collected from March 2021 to February 2022 along the Marche region in the frame of marine toxins official control. Full article

The paper presents the mode in which an accredited laboratory, according to SR EN ISO/IEC 17025, monitors its performance by comparing the results with the results of other laboratories. This article aims to analyse the results of the participation of an accredited laboratory in two ILC schemes, one organized in France and the other in Romania, on cement, discussing only the results of the determinations of the water-soluble hexavalent chromium content. Although chromium (VI) is not present in large amounts in cement, it is responsible for allergic reactions on the skin of workers. Thus, in EU countries, the maximum amount of water-soluble Cr (VI) in bagged cement or in the products based on cement is limited to a maximum of 0.0002% by mass of cement. The Chromium (VI) results obtained by the lab must meet the requirements of the standard SR EN 196-10 for the repeatability and, where appropriate, the reproducibility. Questions about the validity of the results arise when the laboratory obtains satisfactory results in the ILC scheme, but the standard deviation of the scheme is higher than that recommended in the standard method. The elements of the novelty of this paper are the interpretation of water-soluble Cr (VI) results and the use of information obtained from participating in interlaboratory testing schemes tom improve the validity of laboratory results. Full article

This study aims at developing models in analyzing the results of proficiency testing (PT) schemes for a limited number of participants. The models can determine the best estimators of location and dispersion using unsatisfactory results as a criterion by combining: (a) robust and classical estimators; (b) kernel density plots; (c) Z-factors; (d) Monte Carlo simulations; (e) distributions derived from the addition of one or two contaminating distributions and one main Gaussian. The standards ISO 13258:2015, ISO 5725:2:1994, and EN ISO/IEC 17043:2010 are the basis of the analysis. The study describes an algorithm solving the optimization problem for (a) Gaussian, bimodal or trimodal distributions; (b) participating labs from 10 to 30; (c) fraction of the contaminating population up to 0.10; (d) variation coefficient of the main distribution equal to 2; (e) equal standard deviations of all the distributions, and provide figures with the optimal estimators. We also developed a generalized algorithm using kernel density plots and the previous algorithm, which is not subject to restrictions (b)–(e) and implemented in the results of a PT for the 28-day strength of cement with 12–13 participants. Optimal estimators’ figures and the generalized algorithm are helpful for a PT expert in choosing robust estimators. Full article

The use of additive technologies keeps growing. Increasingly, flammable powder materials are also used in additive technologies, and there is a risk of explosion or fire when using them. The current article deals with the determination of fire parameters of a powder sample of polyamide Sinterit PA12 Smoth in accordance with the EN 14034 and EN ISO/IEC 80079-20-2 standards. For that purpose, a sample at a median size of 27.5 µm and a humidity of 0% wt. was used. The measurements showed that the maximum explosion pressure of the PA12 polyamide sample was 6.78 bar and the value of the explosion constant K_st was 112.2 bar·m·s⁻¹. It was not possible to determine the MIT value of the settled dust, since the melting point of polyamide sample is low. The MIT of the dispersed dust was 450 °C. Based on the measured results, it can be stated that the powdered polyamide PA12 poses a risk in terms of explosions and fires. Therefore, when using polyamide PA12 in additive technologies, it is necessary to ensure an effective explosion prevention. Full article

This article deals with the minimum requirements for quality management at Periodical Technical Inspection Stations (PTIS) for road vehicles in the Slovak Republic, as well as in selected countries of the European Union. Specifically, it contains research performed at all of the PTIS in the Slovak Republic with a focus on the established quality management system and the number of employees of companies operating these PTIS, as well as similar research in selected countries of the European Union. Based on the research results, the article contains an assessment of the influence of the implemented quality management system on the operation of PTIS. The analysis of the results showed that 86.7% of PTIs have an established system to meet the minimum requirements for quality management through a documented procedure (internal regulation), and 13.3% of the PTI have the certified quality management system according to the STN EN ISO/IEC 9001 standard. Unfortunately, no PTI in the Slovak Republic has the fulfilment of quality management requirements according to the standard STN EN ISO/IEC 17020. Full article

Q fever is a zoonosis caused by Coxiella burnetii, a Gram-negative pathogen with a complex life cycle and a high impact on public and animal health all over the world. The symptoms are indistinguishable from those belonging to other diseases, and the disease could be symptomless. For these reasons, reliable laboratory tests are essential for an accurate diagnosis. The aim of this study was to validate a novel enzyme-linked immunosorbent assay (ELISA) test, named the Chorus Q Fever Phase II IgG and IgM Kit (DIESSE, Diagnostica Senese S.p.A), which is performed by an instrument named Chorus, a new device in medical diagnostics. This diagnostic test is employed for the detection of antibodies against C. burnetii Phase II antigens in acute disease. Our validation protocol was performed according to the Italian Accreditation Body (ACCREDIA) (Regulation UNI CEI EN ISO/IEC 17025:2018 and 17043:2010), OIE (World Organization for Animal Health), and Statement for Reporting Studies of Diagnostic Accuracy (STARD). Operator performance was evaluated along with the analytical specificity and sensitivity (ASp and ASe) and diagnostic accuracy of the kit, with parameters such as diagnostic specificity and sensitivity (DSp and DSe) and positive and negative predictive values (PPV and NPV), in addition to the repeatability. According to the evaluated parameters, the diagnostic ELISA test was shown to be suitable for validation and commercialization as a screening method in human sera and a valid support for clinical diagnostics. Full article

A 7-min multifamily residue method for the simultaneous quantification and confirmation of 8 phytohormones and 27 acidic herbicides in fruit and vegetables using ultra high-performance liquid chromatography (UHPLC) coupled to tandem mass spectrometry (MS/MS) was developed, validated according to SANTE 12682/2019, and accredited according to UNE-EN-ISO/IEC 17025:2017. Due to the special characteristics of these kinds of compounds, a previous step of alkaline hydrolysis was carried out for breaking conjugates that were potentially formed due to the interactions of the analytes with other components present in the matrix. Sample treatment was based on QuEChERS extraction and optimum detection conditions were individually optimized for each analyte. Cucumber (for high water content commodities) and orange (for high acid and high water content samples) were selected as representative matrices. Matrix-matched calibration was used, and all the validation criteria established in the SANTE guidelines were satisfied. Uncertainty estimation for each target compound was included in the validation process. The proposed method was applied to the analysis of more than 450 samples of cucumber, orange, tomato, watermelon, and zucchini during one year. Several compounds, such as 2,4-dichlorophenoxyacetic acid (2,4-D), 4-(3-indolyl)butyric acid (IBA), dichlorprop (2,4-DP), 2-methyl-4-chlorophenoxy acetic acid (MCPA), and triclopyr were found, but always at concentrations lower than the maximum residue level (MRL) regulated by the EU. Full article

Combine harvesters are an important cause of fires worldwide. The purpose of this work has been to investigate the critical points associated with the risk of fire, identified through a survey distributed to combine owners in Aragon (Spain). Information was collected on the technical characteristics of the machines and, when appropriate, on the characteristics of the generated fires (crop, use of straw chopper, point in which the fire was originated, etc.). Based on the survey data, relationships between the characteristics of the machine and the ignition of a fire were analyzed, and the points of the harvester in which the fire originated were investigated. A statistically significant relationship of fire risk was only found with the number of hectares harvested, in such a way that the risk would be especially high for machines with more than 6000 accumulated ha. 32% of the fires were originated in the engine zone, compared with 31% in the cutting bar and 18% in the bearings and belts. The study was completed with on-site temperature measurements carried out on nine machines in 2018 and 2019, in which temperatures above 250 °C were recorded in the exhaust manifold and in the cutting bar. These temperatures exceeded the ignition thresholds obtained in the flammability studies conducted for wheat residues collected from the harvesters. Full article

Many direct and indirect methods, processes, and sensors available on the market today are used to monitor the occupancy of selected Intelligent Building (IB) premises and the living activities of IB residents. By recognizing the occupancy of individual spaces in IB, IB can be optimally automated in conjunction with energy savings. This article proposes a novel method of indirect occupancy monitoring using CO₂, temperature, and relative humidity measured by means of standard operating measurements using the KNX (Konnex (standard EN 50090, ISO/IEC 14543)) technology to monitor laboratory room occupancy in an intelligent building within the Internet of Things (IoT). The article further describes the design and creation of a Software (SW) tool for ensuring connectivity of the KNX technology and the IoT IBM Watson platform in real-time for storing and visualization of the values measured using a Message Queuing Telemetry Transport (MQTT) protocol and data storage into a CouchDB type database. As part of the proposed occupancy determination method, the prediction of the course of CO₂ concentration from the measured temperature and relative humidity values were performed using mathematical methods of Linear Regression, Neural Networks, and Random Tree (using IBM SPSS Modeler) with an accuracy higher than 90%. To increase the accuracy of the prediction, the application of suppression of additive noise from the CO₂ signal predicted by CO₂ using the Least mean squares (LMS) algorithm in adaptive filtering (AF) method was used within the newly designed method. In selected experiments, the prediction accuracy with LMS adaptive filtration was better than 95%. Full article