Search Results (2,840)

With the growing traffic volume in China, numerous existing highway bridges in seismic zones are constrained by outdated design standards that are inadequate against current seismic requirements. To address this issue, this study proposes a novel reinforcement technique using expansive concrete-filled steel tube (ECFST) for bridge piers. Through combined experimental and numerical investigations on ECFST columns, the effect of expansive agent (EA) content on steel tube strain was systematically examined. The monotonic quasi-static tests were conducted to evaluate the influence of steel tube thickness, concrete strength, reinforcement thickness, and EA content on the ultimate bearing capacity. The proposed method was implemented in a case study involving a reinforced concrete pier, with analysis focused on the “confinement–self-stress coupling mechanism” of ECFST. Results demonstrated good agreement between numerical simulations and experimental data. The optimal EA content was identified as 15%, achieving the most effective reinforcement. ECFST-reinforced piers exhibited significantly enhanced seismic performance, achieving up to 22.6% increase in peak bearing capacity compared to non-expansive concrete filling. While steel tube thickness considerably affected the reinforcement efficiency, concrete strength grade showed minimal impact. This research provides theoretical support and practical design guidelines for seismic retrofitting of similar bridge piers. Full article

Phosphate tailings (PTs) are typical industrial byproducts that can rapidly neutralize soil acidity. However, their acid-neutralizing efficacy, long-term application optimization mechanisms, and high-yield regulation pathways for crops remain unclear. This study conducted a corn-potato crop rotation field trial on acidic soils, investigating the effect of different PT application rates (T: CK, 0 t·ha⁻¹; PTs-1, 6 t·ha⁻¹; PTs-2, 9 t·ha⁻¹; PTs-3, 15 t·ha⁻¹) in a multiple cropping system (C: late autumn potatoes (LAP)-early spring potatoes (ESP)-summer maize (SM)). The results showed that two consecutive applications of 9 t·ha⁻¹ of PTs produced optimal results, increasing the LAP yield by 12.82% and the soil quality by 76.51%, while improving the ESP soil quality by 46.21%. The higher yield was mainly attributed to a significant increase in the soil pH (0.72–1.58 units) and enhanced chemical and biological properties (higher exchangeable calcium (ExCa), exchangeable magnesium (ExMg), the total exchangeable salt base ion (TEB), and catalase (CAT) and urease (UE) content and lower soil exchangeable acidity (EA), exchangeable hydrogen ion (ExH), and exchangeable aluminum (ExAl) levels). Notably, a synchronized increase in the total phosphorus (TP) and total potassium (TK) during LAP cultivation, combined with simultaneous growth of TP, available nitrogen (AN), and available phosphorus (AP) during ESP cultivation, and a significant increase in TP and AP during SM cultivation, effectively promoted crop yield. Furthermore, continuous PT application significantly enriched phosphorus (P)-soluble functional bacteria, such as Actinomycetes and Chloroflexota, and enhanced the stability of bacterial-fungal cross-boundary networks. In summary, optimal acidity levels and favorable soil texture improved soil quality, consequently increasing corn and potato yields. This study reveals for the first time that PTs can substantially increase crop production via a synergistic mechanism involving acid-base balance, structural improvement, and microbial activation. Not only does this provide a novel strategy for rapidly improving acidic soils, but it also establishes a solid theoretical and technical foundation for utilizing PT resources. Full article

This study mapped a wide range of naturally derived odors, including those derived from wood, on the two-dimensional axes of tense arousal (TA) and energetic arousal (EA), and examined whether quadrant differences influenced recovery following stress. In the context of Attention Restoration Theory and biophilic design, the study provided preliminary evidence that olfactory stimuli can be treated as a designable element in a functional and reproducible manner. In Experiment 1, wood flours, wood essential oils, and non-wood oils were mapped based on subjective ratings conducted under identical conditions, and differences in their TA–EA positions were revealed. Ratings of “naturalness” were associated with lower EA, suggesting that quadrant mapping can capture meaningful dimensions of odor perception. In Experiment 2, Hinoki and camphor were selected as contrasting stimuli. Hinoki facilitated initial recovery of autonomic nervous system activity, as shown by lower heart rate compared with no odor, whereas camphor showed no effect. These findings demonstrate that TA–EA quadrant mapping provides a practical framework for olfactory design in indoor environments. Full article

The complex life cycle, high reproductive potential and ability to quickly develop resistance to insecticides are key factors contributing to the destructiveness of the peach–potato aphid (Myzus persicae Sulzer) among pest species. Monitoring its population dynamics at a large scale allows us to better understand M. persicae biology and take relevant measures for pest management. For this purpose, reliable molecular tools are needed. Based on the analysis of 128,362 microsatellite loci, we developed four multiplex assays including 49 comprehensively characterised SSR markers. Internal validation confirmed the species specificity and low genotyping error (e_a = 0.8%, e_l = 0.99%, e_obs = 22.7%) of the assays. A total of 194 alleles were identified (mean = 4 alleles per locus, range = 2–8 alleles per locus) within a group of 365 aphid accessions collected in the Vysočina region (Czechia). The studied aphid population showed the typical characteristics expected of the species with clonal or partially clonal reproduction (heterozygote excess, negative F_IS, moderate-to-high linkage disequilibrium (LD), and distortion of the H-W equilibrium for most of the loci), and did not exhibit any stratification on a spatiotemporal level. Owing to the high discriminatory power of the markers, we discovered that the population sample was founded upon a small number of fundatrices, as only five dominating lineages comprising over 70% of all accessions were identified. In conclusion, this study identified a significant number of new high-quality markers with the high discriminatory power necessary for revealing the population structure and dynamics of M. persicae, which holds considerable potential in both general biological and agricultural research. Full article

The study presents research on the use of modified lignocellulosic biomass as a waste sorbent for the removal of anionic dyes from aqueous solutions. The sawdust used as sorption material was subjected to an acid-base modification and further functionalised by introducing amino groups into the biomass structure. Dynamic sorption experiments were carried out in two reactor types (airlift and column) with two sorbents: sawdust treated under acid-base conditions (S-AB) and sawdust aminated with epichlorohydrin after acid-base treatment and preactivation (S-AB-EA). The anionic dye Reactive Black 5 (RB5) was used as a sorbate. The experiments were carried out at two flow rates (0.1 and 0.5 dm³/h) and two feed concentrations (10 and 50 mg/dm³), maintaining the pH of the solution at 3, as determined in previous studies. The experimental data allowed the maximum sorption capacities of the tested sorbents to be determined under dynamic conditions and were described using the Thomas, Adams-Bohart and Yoon-Nelson models. The results showed that the flow rate, the dye concentration and the reactor type strongly influence the efficiency of dye removal. The highest capacity, 73.89 mg/g, was achieved in the airlift reactor for aminated sawdust and preactivation with epichlorohydrin (S-AB-EA) at a feed concentration of 50 mg/dm³ and a flow rate of 0.1 dm³/h. Full article

Phage therapy has garnered significant attention due to the rise of life-threatening multidrug-resistant pathogenic bacteria and the growing awareness of the transfer of resistance genes between pathogens. Considering this, phage therapy applications are being extended to target plant pathogenic bacteria, such as Erwinia amylovora, which causes fire blight in apple and pear orchards. Understanding the mechanisms involved in phage resistance is crucial for enhancing the effectiveness of phage therapy. Despite the challenges of naturally developing a bacteriophage-insensitive mutant (BIM) of E. amylovora (without traditional mutagenesis methods), this study successfully created a BIM against the podovirus ϕEa46-1-A1. The parent strain, E. amylovora D7, and the BIM B6-2 were extensively compared at genomic, transcriptomic, and phenotypic levels. The phenotypic comparison included the metabolic behavior, biofilm formation, and in planta evaluations of pathogenicity. The results revealed a mutation in strain B6-2 in the rcsB gene, which encodes a second regulator in the Rcs two-component phosphorelay system (TCS). This mutation resulted in significant changes in the B6-2 BIM, including downregulation of amylovoran gene expression (e.g., an average log₂ fold change of −4.35 across amsA-L), visible alterations in biofilm formation, increased sensitivity to antibiotics (22.4% more sensitive to streptomycin), and a loss of pathogenicity as assessed in an apple seedling virulence model in comparison to the wildtype strain. The findings presented in this study highlight the critical role of the Rcs phosphorelay system in phage resistance in E. amylovora. Based on these findings, we have proposed a model that explains the effect of the B6-2 rcsB mutation on the Rcs phosphorelay system and its contribution to the development of phage resistance in E. amylovora. Full article

Background: In pediatric age, the central airways are more flexible and mobile, with tracheal and bronchial walls easily tending to collapse, allowing partial or complete occlusion of the lumen: a situation described as tracheobronchomalacia (TBM). This is a condition that causes an increase in intrathoracic pressure that may accentuate airway collapse, and a biphasic or barking cough appears. Objectives: Although TBM is relatively frequent in pediatric age, the diagnostic criteria and subsequent treatment do not follow well-standardized criteria and often vary from pediatric center to center. Therefore, there is a need to standardize diagnostic procedures and the resulting medical or surgical treatments. Methods: We therefore organized a day of meetings to talk about TBM, inviting all Italian pediatricians and pediatric surgeons who diagnose and treat patients with this pathology on a daily basis. Results: This work, collecting all the meeting interventions, is a compendium that deals with all aspects of TBM, emphasizing the most correct criteria to diagnose and therefore best treat each pediatric patient with this clinical condition. We give particular emphasis to the need to perform static and dynamic videobronchoscopy (S/DVBS) to verify the patency of the tracheal lumen, so as to evaluate the severity of TBM. Conclusions: this work deals with TBM in all its diagnostic and treatment aspects and can be a valid help for all pediatricians who treat these patients. Full article

The aviation sector faces increasing pressure to address climate change as its contribution to global CO₂ emissions continues to rise. This study investigates how passengers’ awareness of environmental issues and perceptions of sustainable airline practices affect their Green Air Travel Behavior (GTB). Drawing upon the Theory of Planned Behavior (TPB) and extending it with constructs such as Environmental Awareness (EA), Perceived Service Quality (PSQ), and Green Trust (GT), the research examines their impact on GTB. Using a quantitative design, data were collected from 300 airline passengers and analyzed with Structural Equation Modeling (SEM). Results reveal that EA strongly influences PSQ, GT, Attitude (ATT), and Intention (ITN), highlighting its role as a key antecedent. PSQ significantly enhances GT, while both GT and ATT directly predict GTB. However, the effect of ITN on GTB was not significant, indicating an intention–behavior gap. The findings underscore the importance of awareness, trust, and service quality in promoting sustainable air travel, while also pointing to barriers that hinder intentions from becoming actions. Theoretically, the study extends TPB within green aviation, and practically, it provides guidance for airlines and policymakers seeking to advance SDG 13: Climate Action through sustainable air travel strategies. Full article

Breast cancer is the most common cancer among women in Canada. Its presentation and outcomes vary significantly by race/ethnicity. This study explores breast cancer incidence, age at diagnosis, stage, subtype, and mortality, comparing Black and White women aged 20 years and older, using the 2011 and 2016 Canadian Census Health and Environment Cohorts databases. Black women were disaggregated into Caribbean, Central/West African (C/WA), Southern/East African (S/EA), and “Other” ancestry groups. The Black female study population had a lower mean age (43.0 years) than the White (50.5 years). Black women had lower overall age-standardized breast cancer incidence than White women. The age-specific incidence in Black women ages 30–39 of Caribbean origin was higher (RR 95% CL, 1.36, 1.04–1.79; 58.7 vs. 43.1 cases/100,000 person-years) than in White. White women had 14.6% of cases diagnosed at ages 20–49 compared to over 50% in Black women of C/WA and S/EA origins, with highest proportions of diagnoses occurring at least 10 years earlier among Black women (C/WA 46, S/EA 48, Caribbean 57, White 67). Proportions of prognostic stage I diagnoses were less common among Black vs. White women (53.2% vs. 65.9%, p < 0.0001), and triple negative breast cancer was more frequent among Black women (17.1% vs. 9.9%, p < 0.0001), particularly those of Central/West African ancestry (21.8%). Higher age-specific mortality was observed among Black women with Caribbean origins aged 40–49 (RR 95% CL, 1.70, 1.19–2.42) and 50–59 (RR 95% CL, 1.42, 1.08–1.88) compared to White women. Breast cancer characteristics and outcomes vary substantially by ancestry within Canada’s Black population. Tailored screening strategies accounting for earlier onset and aggressive subtypes may help mitigate disparities. Full article

Recently, the state of the art of aerogels (AGs) has been reviewed, reporting first on their classification, based on the chemical origin of their precursors and the different methods existing to prepare them. Additionally, AGs of inorganic origin (IAGs) were contemplated, deeply discussing the properties, specific synthesis, and possible uses of silica and metal oxide-based AGs, since they are the most experimented and patented AGs already commercialized in several sectors. In this second part review, IAGs are examined again, but chalcogenide and metals AGs (CAGs and MAGs) are debated, since they are still too little studied, patented, and marketed, despite their nonpareil properties and vast range of possible applications. First, to give readers unaware of the previous work on AGs, a background about IAGs, all their main subclasses have been reported and their synthesis, including sol–gel, epoxide addition (EA), and dispersed inorganic (DIS) methods, as well as procedures involving the use of pre-synthesized nanoparticles as building blocks, have been discussed. Morphology and microstructure images of materials prepared by such synthetic method have been supplied. Conversely, the methods needed to prepare CAGs and MAGs, topics of this study, have been debated separately in the related sections, with illustrative SEM images. Their possible uses, properties, and some comparisons of their performance with that of other AGs and not AG materials traditionally tested for the same scopes, have also been disserted, reporting several case studies in reader-friendly tables. Full article

Background/Objectives: Exercise-induced hypertension (EIH) in runners predisposes them to cardiovascular diseases, including myocardial hypertrophy, arrhythmias, and coronary artery disease. Blood flow restriction (BFR) training has been reported to exert non-pharmacological benefits in runners with EIH by improving blood pressure, myocardial workload, and cardiorespiratory fitness. The purpose of this study was to investigate whether changes in myocardial structure and function accompany these effects of BFR training in middle-aged runners with EIH. Methods: Participants who exhibited a maximal systolic blood pressure of ≥210 mmHg during an exercise stress test were assigned either to a BFR training group (BFRTg, n = 15) or to a control group without BFR training (non-BFRTg, n = 14). The BFRTg underwent a two-month BFR training program, performed twice per week for 20 min per session. Cardiac structure and function were evaluated before and after the intervention, and exercise stress test data were obtained from secondary sources of a previous study. Results: Compared with controls, the BFR group showed lower maximal exercise SBP, longer exercise duration, and higher VO_2max. Echocardiography revealed reduced interventricular septal thickness and improved diastolic indices (higher E′/A′, lower E/E′), while systolic function remained unchanged. Conclusions: In conclusion, reductions in septal thickness and improvements in diastolic function induced by blood flow restriction training in runners with exercise-induced hypertension suggest a favorable cardiac adaptation, accompanied by concurrent improvements in exercise blood pressure and cardiorespiratory fitness. Full article

Background: Some studies have suggested that physical fitness and body composition may influence individual and collective performance. However, it is necessary to be able to define the relationships between these variables in soccer players of different ages. Objective: To determine the relation between physical fitness level, body composition, and somatotype in female youth soccer players in response to age. Materials and methods: A total of 56 players were evaluated: 19 early adolescents (EA–U13) with a body mass of 48.35 ± 5.67 kg and a height of 157.63 ± 5.55 cm, 21 middle adolescents (MA–U15) with a body mass of 54.02 ± 5.96 kg and a height of 160.37 ± 5.25 cm and 16 late adolescents (LA–U17) with a body mass of 55.37 ± 6.15 kg and a height of 162.39 ± 5.77 cm. The physical fitness tests were: Squat Jump (SJ), Countermovement Jump (CMJ), Countermovement Jump with Arms (CMJA), Single Leg Countermovement Jump, COD-Timer 5-0-5, COD-Timer 5+5, Speed 15 m, Hamstring Strength, and Running-Based Anaerobic Sprint Test (RAST). The International Society for the Advancement of Kinanthropometry (ISAK) protocols were used to determine anthropometric measurements (skinfolds, circumferences, bone diameters), and the Heath-Carter method was used to assess body composition and somatotype, with z-scores calculated using the Phantom strategy. Results: The analysis revealed that the most significant differences between groups were observed in general anthropometric measurements (ω² = 0.84), followed by sitting height (ω² = 0.51) and percentage of body fat according to Carter’s method (ω² = 0.24), all with large and statistically significant effect sizes (p < 0.05). Larger muscle and bone dimensions, especially in the hip, thigh, and calf, are closely related to better strength, power, and initial sprint speed performance in female soccer players. Conclusions: This study reaffirms that muscle mass is a key predictor of athletic performance, along with strength at high speeds, promoting improvements in power and sprinting in the initial meters. Adiposity is a limiting factor for youth soccer players. Age progression and biological maturation favor the development of the mesomorphic profile, optimizing strength and power. Full article

Hemodynamics significantly impact the biology of endothelial cells (ECs) lining the blood vessels. ECs are exposed to various hemodynamic forces, particularly frictional shear stress from flowing blood. While physiological flows are critical for the normal functioning of ECs, abnormal flow dynamics, known as disturbed flows, may trigger endothelial dysfunction leading to atherosclerosis and other vascular conditions. Such flows can occur due to sudden geometrical variations and vascular abnormalities in the cardiovascular system. In the current study, a microfluidic system was used to investigate the impact of different flow conditions (i.e, normal vs. disturbed) on ECs in vitro. We particularly explored the relationship between specific flow patterns and cellular pathways linked to oxidative stress and inflammation related to atherosclerosis. Here, we utilized a 2D cell culture perfusion system featuring an immortalized human vascular endothelial cell line (EA.hy926) connected to a modified peristaltic pump system to generate either steady laminar flows, representing healthy conditions, or disturbed oscillatory flows, representing diseased conditions. EA.hy926 were exposed to an oscillatory flow shear stress of 0.5 dynes/cm² or a laminar flow shear stress of 2 dynes/cm² up to 24 h. Following flow exposure, cells were harvested from the perfusion chamber for quantitative PCR analysis of gene expression. Reactive oxygen species (ROS) generation under various shear stress conditions was also measured using DCFDA/H2DCFDA fluorescent assays. Under oscillatory shear stress flow conditions (0.5 dynes/cm²), EA.hy926 ECs showed a 3.5-fold increase in the transcription factor nuclear factor (NFκ-B) and a remarkable 28.6-fold increase in cyclooxygenase-2 (COX-2) mRNA expression, which are both proinflammatory markers, compared to static culture. Transforming growth factor-beta (TGFβ) mRNA expression was downregulated in oscillatory and laminar flow conditions compared to the static culture. Apoptosis marker transcription factor Jun (C-Jun) mRNA expression increased in both flow conditions. Apoptosis marker C/EBP homologous protein (CHOP) mRNA levels increased significantly in oscillatory flow, with no difference in laminar flow. Endothelial nitric oxide synthase (eNOS) mRNA expression was significantly decreased in cells exposed to oscillatory flow, whereas there was no change in laminar flow. Endothelin-1 (ET-1) mRNA expression levels dropped significantly by 0.5- and 0.8-fold in cells exposed to oscillatory and laminar flow, respectively. ECs subjected to oscillatory flow exhibited a significant increase in ROS at both 4 and 24 h compared to the control and laminar flow. Laminar flow-treated cells exhibited a ROS generation pattern similar to that of static culture, but at a significantly lower level. Overall, by exposing ECs to disturbed and normal flows with varying shear stresses, significant changes in gene expression related to inflammation, endothelial function, and oxidative stress were observed. In this study, we present a practical, optimized system as an in vitro model that can be employed to investigate flow-associated diseases, such as atherosclerosis and aortic aneurysm, thereby supporting the understanding of the underlying molecular mechanisms. Full article

Compost amendments are widely recognized as an effective strategy for improving soil quality, modulating enzyme activities, and enhancing nitrogen cycling. Urease, a key enzyme in nitrogen transformation, is characterized by kinetic parameters such as the maximum reaction rate (V_max) and Michaelis constant (K_m), as well as thermodynamic attributes including temperature sensitivity (Q₁₀), activation energy (E_a), enthalpy change (ΔH), Gibbs free energy change (ΔG), and entropy change (ΔS). However, how different compost sources regulate urease kinetics, thermodynamics, and nitrogen availability remains poorly understood. In this study, we evaluated the effects of three compost amendments—mushroom residue (MR), mushroom residue–straw mixture (MSM), and leaf litter (LL)—on urease kinetics and thermodynamics in a temperate agroecosystem. The MSM treatment significantly enhanced urea hydrolysis capacity and catalytic efficiency. In contrast, LL treatment resulted in the highest Km value, indicating a substantially lower enzyme-substrate affinity. Furthermore, MSM reduced the E_a and increased the thermal stability of urease, thereby supporting enzymatic performance under fluctuating temperatures. Collectively, our findings highlight that compost composition is a critical determinant of urease function and nitrogen turnover. By elucidating the coupled kinetic and thermodynamic responses of urease to compost inputs, this study provides mechanistic insights to guide optimized soil management and sustainable nitrogen utilization in temperate agricultural systems. Full article

Laser cladding offers distinct advantages over traditional manufacturing methods, including low heat input, minimal dilution ratio, dense clad layers, and robust bonding. It is widely employed for surface strengthening of metals to enhance performance and repair failed components, thereby reducing material waste. This study investigates laser cladding repair of EA4T steel, focusing on examining the effects of laser power, scanning speed, and powder feed rate on melt pool dilution ratio and shape factor during cladding of IN718 material onto EA4T steel substrate. Orthogonal experiments were conducted to investigate the combined effects of different process parameters on dilution rate and shape factor. Optimal process parameters were determined by comprehensively evaluating melt pool cross-sectional morphology and internal defects. Based on this, theoretical lap calculations were performed, and the optimal theoretical lap ratio was obtained through experiments. Experiments indicated that the influence of process parameter variations on molten pool morphology parameters is not linear; the combined effects of all factors must be comprehensively considered. Full article