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Review

39 Citations

8,505 Views

19 Pages

Compendium of Plant-Specific CRISPR Vectors and Their Technical Advantages

Anshu Alok,
Hanny Chauhan,
Santosh Kumar Upadhyay,
Ashutosh Pandey,
Jitendra Kumar and
Kashmir Singh

Life2021, 11(10), 1021;https://doi.org/10.3390/life11101021

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28 September 2021

CRISPR/Cas mediated genome editing is a revolutionary approach for manipulating the plant genome. However, the success of this technology is highly dependent on selection of a specific vector and the other components. A plant-specific CRISPR/Cas vect...

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Communication

12 Citations

5,336 Views

11 Pages

PARA: A New Platform for the Rapid Assembly of gRNA Arrays for Multiplexed CRISPR Technologies

Guoliang Yuan,
Stanton Martin,
Md Mahmudul Hassan,
Gerald A. Tuskan and
Xiaohan Yang

Cells2022, 11(16), 2467;https://doi.org/10.3390/cells11162467

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9 August 2022

Multiplexed CRISPR technologies have great potential for pathway engineering and genome editing. However, their applications are constrained by complex, laborious and time-consuming cloning steps. In this research, we developed a novel method, PARA,...

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