Search Results (40)

This study utilized a twisted wire rotating arc cladding method to in situ fabricate a Fe-containing multi-principal element alloy (HPEA) coating derived from NiCrCoTiMo stranded wire on 45 steel (equivalent to AISI 1045 steel). The macroscopic morphology, microstructure, mechanical properties, and electrochemical corrosion behavior of the prepared coatings were examined. The coating exhibited no visible cracks or pores and displayed a dual-phase face-centered cubic (FCC) + body-centered cubic (BCC) structure, with an average grain size of 78 μm for the FCC phase and 1 μm for the BCC phase. The microhardness of the coating is approximately 381.3 HV_0.1. Compared to 45 steel, the coating’s coefficient of friction (COF) decreased from 0.6265 to 0.5125, representing an 18.2% reduction. The calculated wear rate of the coating was 1.47 × 10⁻⁵ mm³/N·m, approximately six times lower than that of 45 steel (8.93 × 10⁻⁵ mm³/N·m). Electrochemical testing revealed that the coating’s open-circuit potential (OCP) was −0.405 V vs. the saturated calomel electrode (SCE), with a corrosion potential (E_corr) of −0.556 V vs. SCE and a corrosion current density (I_corr) of 4.458 × 10⁻⁶ A/cm². In comparison, 45 steel exhibited an OCP of −0.582 V vs. SCE, with corrosion parameters of E_corr = −0.840 V vs. SCE and I_corr = 1.302 × 10⁻⁵ A/cm². These results demonstrate the superior corrosion resistance and wear performance of the coating, underscoring its potential for applications in challenging environments that demand enhanced material durability. Full article

Mutualistic interactions between plants and beneficial fungi rely on extensive transcriptional reprogramming in both partners, yet the underlying regulatory mechanisms coordinating these responses remain incompletely understood. Here, we combined a transcriptomics analysis with a gene regulatory network (GRN) inference to dissect the interaction between Arabidopsis thaliana and the growth-promoting fungus Trichoderma atroviride. At an early but established stage of colonization (72 h post-inoculation), we identified widespread transcriptional changes in both of the organisms, including host activation of hypoxia, stress and root development-related pathways in Arabidopsis, and fungal reprogramming of membrane transport and primary metabolism. Using DNA-binding motifs and GENIE3-based regulatory inference, we reconstructed interaction-specific GRNs for each species. The subnetworks focused on the main differentially expressed biological processes and uncovered ERF-, WRKY-, NAC- and DOF-centered modules linking hypoxia responses with developmental remodeling in the plant, whereas the putative orthologs of TFs involved in developmental and stress-related TFs in fungi, such as CrzA, RME1, NsdC, PacC and RPN4, formed a regulatory core coordinating fungal transport and metabolic adjustment. In parallel, we uncovered contrasting sRNA dynamics between the partners. While the Arabidopsis sRNA changes were limited, T. atroviride exhibited a strong induction of 20–22 nt sRNAs, including a small set of high-confidence sRNA–mRNA interactions targeting host genes involved in root function and immunity. Together, our results extend previous pathway-based descriptions of the Arabidopsis–Trichoderma mutualism and provide a systems-level, testable framework for how coordinated regulatory programs in both of the partners support the interaction. Full article

Cryptococcus neoformans is a pathogenic yeast and the leading cause of cryptococcosis in humans. The calcium-calcineurin signaling pathway plays a central role in stress adaptation and virulence. To identify the uncharacterized regulators of fungal adaptation, we utilized an integrative systems biology approach, combining differential gene expression and network analysis using transcriptomic data from three key components of the calcium-calcineurin pathway (Cna1, Crz1, and Pmc1). Our workflow identified the CNAG_00522 gene product, which we designated tricalbin 1 (TCB1) due to its conserved calcium and lipid-binding C2 domains. TCB1 expression was found to be regulated by both Cna1 and Pmc1. Network analyses positioned Tcb1 as a bottleneck linking general stress response and cellular processes. Further molecular characterization confirmed that TCB1 expression is temperature and calcium-responsive. Functional studies of the tcb1Δ mutant revealed an enlarged capsule, increased GXM shedding, and enhanced viability under host-mimicking conditions. However, phenotypic screening demonstrated that the tcb1Δ mutant does not display sensitivity to cell wall or osmotic stressors, and TCB1 deletion did not attenuate virulence in the Tenebrio larval model. These findings suggest that TCB1 functions as a specialized regulator of fungal growth at 37 °C, capsule size, and GXM shedding. This study validates our integrative approach for guiding the identification of these complex regulators. Full article

The cell wall integrity pathway is activated in response to cell wall stress (CWS). The defense system in aspergilli employs three transcription factors—RlmA, MsnA, and CrzA—which also facilitate adaptation to various abiotic stressors and involve alterations in cytosolic osmolyte composition and membrane lipid profiles. However, their role in adaptation to CWS remains unclear. In Aspergillus niger, CWS induced by Congo red and calcofluor white caused a pronounced cessation of apical growth, accompanied by hyphal globular swelling and an increase in chitin and glucan content in the cell wall. Regarding the osmolyte composition, which predominantly consists of low levels of glycerol and mannitol, glycerol levels were reduced under CWS. Neither the composition nor the amounts of membrane and storage lipids changed following CWS; however, the degree of unsaturation of phospholipids increased due to a higher proportion of linolenic acid, potentially enhancing membrane fluidity. These minor rearrangements of membrane lipids and osmolytes do not confirm their involvement in the adaptation to CWS induced by Congo red and calcofluor white, contrary to previous assumptions based on studies of cell wall integrity pathways. Full article

Combustion at the meso-scale is constrained by large surface-to-volume ratios that shorten residence time and intensify wall heat loss. We perform steady, three-dimensional CFD of two asymmetric vortex combustors: Model A (compact) and Model B (larger-volume) over inlet-air mass flow rates $\dot{m}$ (40–170 mg s⁻¹) and equivalence ratios ϕ (0.7–1.5), using an Eddy-Dissipation closure for turbulence–chemistry interactions. A six-mesh independence study (the best mesh is 113,133 nodes) yields ≤ 1.5% variation in core fields and ~2.6% absolute temperature error at a benchmark station. Results show that swirl-induced CRZ governs mixing and flame anchoring: Model A develops higher swirl envelopes (S up to ~6.5) and strong near-inlet heat-flux density but becomes breakdown-prone at the highest loading; Model B maintains a centered, coherent Central Recirculation Zone (CRZ) with lower $u_{\theta }$ (~3.2 m s⁻¹) and S ≈ 1.2–1.6, distributing heat more uniformly downstream. Peak flame temperatures (~2100–2140 K) occur at ϕ ≈ 1.0–1.3, remaining sub-adiabatic due to wall heat loss and dilution. Within this regime and $\dot{m}$ ≈ 85–130 mg s⁻¹, the system balances intensity against flow coherence, defining a stable, thermally efficient operating window for portable micro-power and thermoelectric applications. Full article

Candida auris has emerged as a global public health threat due to its high mortality rates, multidrug resistance, and rapid transmission in healthcare settings. This study reports the first documented cases of C. auris candidemia in Portugal, comprising eight isolates from candidemia and colonised patients admitted to a major hospital in northern Portugal in 2023. Whole-genome sequencing (WGS) was performed to determine the phylogenetic relationships of the isolates, which were classified as belonging to Clade I. Genome sequencing also enabled the detection of missense mutations in antifungal resistance genes, which were correlated with antifungal susceptibility profiles determined according to EUCAST (European Committee on Antimicrobial Susceptibility Test) protocols and guidelines. All isolates exhibited resistance to fluconazole and amphotericin B according to the recently established EUCAST epidemiological cut-offs (ECOFFs). Most of the isolates showed a resistant phenotype to anidulafungin and micafungin. All isolates were resistant to caspofungin. Missense mutations identified included Y132F in ERG11, E709D in CDR1, A583S in TAC1b, K52N and E1464K in SNQ2, K74E in CIS2, M192I in ERG4, a novel mutation S237T in CRZ1, and variants in GCN5, a gene involved in chromatin remodelling and stress-response regulation. Identifying known and novel mutations highlights the evolution of antifungal resistance mechanisms in C. auris. These findings underscore the need for further research to understand C. auris resistance pathways and to guide effective clinical management strategies. Full article

Confinement with a rectangular cross-section is commonly used to simulate the role of a swirl combustor, yet its effect on swirling flows remains poorly understood. This study investigates the influence of confinement on the isothermal flow field of a counter-rotating swirler. A particle image velocimetry (PIV) system was employed to measure the swirling flow field under varying confinement ratios at an air pressure drop equivalent to 3% of atmospheric pressure. The results reveal two distinct flow patterns, delineated by a critical confinement ratio of approximately 8.92. Detailed analyses of the velocity components, contour distributions, and Reynolds shear stresses were conducted. The two flow patterns are attributed to the wall attachment effect and swirling intensity, respectively. Furthermore, the results confirm that the swirling flow field is primarily governed by the confinement ratio. Full article

Meso-scale combustors face persistent challenges in sustaining stable combustion and efficient heat transfer due to high surface-to-volume ratios and attendant heat losses. In contrast, larger outlet diameters exhibit weaker recirculation and more diffused temperature zones, resulting in reduced combustion efficiency and thermal confinement. The behavior of non-premixed flames in meso-scale combustor has been investigated through a comprehensive numerical study, utilizing computational fluid dynamics (CFD) under stoichiometric natural gas (methane)–air conditions; three outlet configurations (6 mm, 8 mm, and 10 mm) were analysed to evaluate their impact on temperature behaviour, vortex flow, swirl intensity, and central recirculation zone (CRZ) formation. Among the tested geometries, the 6 mm outlet produced the most robust central recirculation, intensifying reactant entrainment and mixing and yielding a sharply localised high-temperature core approaching 1880 K. The study highlights the critical role of geometric parameters in governing heat release distribution, with the 6 mm configuration achieving the highest exhaust temperature (920 K) and peak wall temperature (1020 K), making it particularly suitable for thermoelectric generator (TEG) integration. These findings underscore the interplay between combustor geometry, flow dynamics, and heat transfer mechanisms in meso-scale systems, providing valuable insights for optimizing portable power generation devices. Full article

The desert locust (Schistocerca gregaria) represents one of the most destructive agricultural pests globally, renowned for its ability to form massive swarms that can devastate crops and threaten food security across vast regions. Despite the widespread application of the CRISPR/Cas9 gene-editing system in several insect orders, its utilization in locusts, particularly in the desert locust, has remained relatively unexplored. We established a CRISPR/Cas9-mediated gene-editing workflow for the desert locust using gene encoding for neuropeptide corazonin (Crz) as a target. We also analyzed the phenotypic and physiological characteristics of the mutant using paraffin sectioning, HE staining, and chitin staining techniques. Our findings revealed that while Crz knockout desert locusts were viable and maintained normal fertility, they exhibited striking phenotypic alterations, including albinism and a significant reduction in cuticle thickness. These observations not only highlight the functional role of Crz in pigmentation and cuticle development but also underscore the potential of CRISPR/Cas9 as a powerful tool for dissecting gene function in locusts. Furthermore, the successful application of CRISPR/Cas9 in desert locusts also paves the way for similar genetic studies in other non-model insects, expanding the scope of functional genomics in entomology. Full article

Corazonin (Crz) is widely found in insects and crustaceans. In insects, Crz participates in the regulation of various physiological activities, including heartbeat, body color change, molting, and reproduction. However, the physiological effects of Crz in crustaceans remain largely unclear. In this study, the cDNAs encoding Crz and its putative receptor were isolated from the mud crab Scylla paramamosain. Tissue distribution analysis revealed that Sp-Crz was predominantly expressed in neural tissues, while its receptor (Sp-CrzR) was widely expressed in S. paramamosain, with a high expression level in the Y-organ. During ovarian development, Sp-Crz expression in the eyestalk ganglion was upregulated at the early and late vitellogenic stages, whereas its expression level in the cerebral ganglion displayed an initial downregulation at the early stage, followed by a remarkable upregulation at the late vitellogenic stage. The expression level of Sp-CrzR mRNA in the ovary increased significantly at the late vitellogenic stage. However, an opposite expression pattern was observed in the hepatopancreas and Y-organ. The immunohistochemistry result showed that Sp-Crz was distributed in the cells of the lamina ganglionaris, the medulla interna, and the X-organ of the eyestalk ganglion. It was revealed that the level of Sp-Vg in the hepatopancreas was not affected by the addition of Sp-Crz in vitro. However, the expression of Sp-VgR in ovarian explants was significantly induced by 6 h treatment with Sp-Crz at a concentration of 1 nM. In addition, the level of Sp-VgR in the ovary was significantly upregulated by 12 h injection of Sp-Crz. After long-term administration of Sp-Crz, the expression of Sp-VgR in the ovary, the E₂ content in hemolymph, the oocyte diameter, and the gonadosomatic index of S. paramamosain were significantly increased. In summary, these findings collectively indicate that the Sp-Crz signaling system participates in regulating the ovarian development of the mud crab. This study provides a new insight into the biological function of Crz during the ovarian development of the mud crab, which is of great significance for the sustainable development and utilization of mud crab resources. Full article

Alcohol consumption and addiction remain global health concerns, with significant loss of productivity, morbidity, and mortality. Drosophila melanogaster, a widely used model organism, offers valuable insights into the genetic and neuronal mechanisms underlying ethanol-induced behaviors (EIBs) such as sedation, recovery, and tolerance. This narrative review focuses on studies in the Drosophila model system suggesting an association between circadian rhythm genes as modulators of ethanol tolerance. Mutations in these genes disrupt both the circadian cycle and tolerance, underscoring the interplay between circadian rhythm and ethanol processing although the exact mechanisms remain largely unknown. Additionally, genes involved in stress response, gene expression regulation, neurotransmission, and synaptic activity were implicated in ethanol tolerance modulation. At the neuronal level, recent studies have highlighted the involvement of corazonin (CRZ) and neuropeptide F (NPF) neurons in modulating EIBs. Understanding the temporal dynamics of tolerance development is crucial for describing the molecular basis of ethanol tolerance. Ultimately, insights gained from Drosophila studies hold promise for elucidating the neurobiological underpinnings of alcohol use disorders and addiction, contributing to more effective interventions and treatments. Full article

The integration of communication and sensing (ICAS) in optical networks is an inevitable trend in building intelligent, multi-scenario, application-converged communication systems. However, due to the impact of nonlinear effects, co-fiber transmission of sensing signals and communication signals can cause interference to the communication signals, leading to an increased bit error rate (BER). This paper proposes a noncoherent solution based on the alternate polarization chirped return-to-zero frequency shift keying (Apol-CRZ-FSK) modulation format to realize a 4 × 100 Gbps dense wavelength division multiplexing (DWDM) optical network. Simulation results show that compared to traditional modulation formats, such as chirped return-to-zero frequency shift keying (CRZ-FSK) and differential quadrature phase shift keying (DQPSK), this solution demonstrates superior resistance to nonlinear effects, enabling longer transmission distances and better transmission performance. Moreover, to meet the transmission requirements and signal sensing and recognition needs in future optical networks, this study employs the Inception-ResNet-v2 convolutional neural network model to identify three modulation formats. Compared with six deep learning methods including AlexNet, ResNet50, GoogleNet, SqueezeNet, Inception-v4, and Xception, it achieves the highest performance. This research provides a low-cost, low-complexity, and high-performance solution for signal transmission and signal recognition in high-speed optical networks designed for integrated communication and sensing. Full article

Alternaria alternata, a causal agent of pear black spot, can recognize and respond to physicochemical signals from fruit surfaces through an intricate signaling network to initiate infection. Crz1 is an important transcription factor downstream of the calcium signaling pathway. In this study, we first investigated the infection structure formation process of the wild type (WT) and ΔAaCrz1 strains induced by the cuticular wax of the “Zaosu” pear by microscopic observation. We found that the infection process was delayed and the rate of appressorium formation and infection hyphae formation was significantly decreased in the ΔAaCrz1 strain. RNA-seq of WT and ΔAaCrz1 strains was analyzed after 6 h of induction with pear wax. A total of 893 up-regulated and 534 down-regulated genes were identified. Among them, genes related to cell wall degrading enzymes, ABC transporters, and ion homeostasis were down-regulated, and the autophagy pathway was induced and activated. In addition, disruption to the intracellular antioxidant system was also found after AaCrz1 knockdown. In summary, this study provides new information on the mechanism of the transcription factor AaCrz1 in the regulation of infection structure formation of A. alternata induced by pear peel wax signal, which can be used to develop new strategies for controlling fungal diseases in the future. Full article

The neuropeptide corazonin (Crz) exerts diverse physiological effects in insects, yet its role in crustaceans remains elusive. The abundant expression of Crz receptor (CrzR) in the Y-organs of several crustaceans suggests a potential involvement of Crz in regulating ecdysteroid synthesis. In this study, we examined the effects of PtCrz on ecdysteroid synthesis during the molting period of Portunus trituberculatus through PtCrz treatments and PtCrzR silencing. Our results showed that PtCrz peptide stimulates ecdysteroid levels and the gene expression involved in ecdysteroidogenesis both in vitro and in vivo, whereas dsPtCrzR treatments had opposite effects on ecdysteroid levels and associated gene expression. Thus, our study suggests that PtCrz may modulate ecdysteroid synthesis via Y-organ-expressed PtCrzR. Furthermore, we also discovered the involvement of PtCrz/PtCrzR signaling in regulating PtETH expression. Notably, the inhibitory effect of dsPtCrzR on ecdysteroid synthesis or PtETH expression can be reversed by PtCrz treatment, suggesting the potential existence of multiple receptors for PtCrz. This study provides new insights into the function of crustacean Crz and, for the first time, elucidates the presence of a neuropeptide that can stimulate ecdysteroid synthesis in crustaceans. Full article

Background: The pathogenic fungus Candida albicans is a leading agent of death in immunocompromised individuals with a growing trend of antifungal resistance. Methods: The purpose is to induce resistance to drugs in a sensitive C. albicans strain followed by whole-genome sequencing to determine mechanisms of resistance. Strains will be assayed for pathogenicity attributes such as ergosterol and chitin content, growth rate, virulence, and biofilm formation. Results: We observed sequential increases in ergosterol and chitin content in fluconazole-resistant isolates by 78% and 44%. Surface thickening prevents the entry of the drug, resulting in resistance. Resistance imposed a fitness trade-off that led to reduced growth rates, biofilm formation, and virulence in our isolates. Sequencing revealed mutations in genes involved in resistance and pathogenicity such as ERG11, CHS3, GSC2, CDR2, CRZ2, and MSH2. We observed an increase in the number of mutations in key genes with a sequential increase in drug-selective pressures as the organism increased its odds of adapting to inhospitable environments. In ALS4, we observed two mutations in the susceptible strain and five mutations in the resistant strain. Conclusion: This is the first study to induce resistance followed by genotypic and phenotypic analysis of isolates to determine mechanisms of drug resistance. Full article