Search Results (584)

Lychee (Lychee chinensis Sonn.) is a tropical fruit highly appreciated for its vivid red color, sweet flavor, and nutritional properties. However, it is highly perishable, with postharvest losses often due to oxidative browning and dehydration. This study evaluated the organic olive oil coating (OC), a natural lipidic system with the potential to act as a precursor for organogel development, combined with polyamide/polyethylene (PA/PE) packaging under passive modified atmosphere. Fruits were harvested at commercial maturity and divided into two groups: OC-treated and untreated control (CTR). Both groups were stored at 5 ± 1 °C and 90 ± 5% relative humidity and analyzed on days 0, 3, 6, and 9. The OC-treated fruits showed significantly better retention of physical, chemical, microbiological, and sensory qualities. The coating reduced oxidative stress and enzymatic browning, preserving color and firmness. The PA/PE packaging regulated gas exchange, lowering oxygen levels and delaying respiration and ripening. As a result, OC fruits had lower weight loss, a slower increase in browning index and maturity index, and better visual and sensory scores than the CTR group. This dual strategy proved effective in extending shelf life while maintaining the fruit’s appearance, flavor, and nutritional value. It represents a sustainable and natural approach to enhancing the postharvest stability of lychee. Full article

Background/Objectives: Tamoxifen, a selective estrogen receptor modulator widely used as an adjunct in the treatment of breast cancer, has known effects on bone metabolism, although its impact on osseointegration and cellular responses during early bone healing remains unclear. Understanding these effects is essential given the increasing use of dental implants in cancer survivors. The study aimed to observe the influence of tamoxifen on human osteosarcoma (SAOS-2) cells lines, as well on the osseointegration of titanium implants in ovariectomized female rats. Methods: SAOS-2 cells were incubated with Dulbecco’s modified growth medium. Six titanium (Ti) disks were used at each time point. The samples were divided into groups with the presence (TAM, n = 36) or not (CTR, n = 36) of tamoxifen in a concentration of 2 μM. In vivo, 72 animals were divided in groups with bilateral ovariectomy or SHAM and tamoxifen administration or not (15 mg/kg). Cell viability, mineralization rate, and collagen synthesis were assessed, as well as bone/implant contact (BIC) and bone ingrowth (BIN). Results: Tamoxifen caused a decrease in SAOS-2 viability, although an increase in the mineralization rate was observed. In vivo, the TAM groups presented higher BIC and BIN when compared to their control, but a lower percentage of mature collagen cells. Conclusions: Based on our findings, in vitro, the therapy with TAM slightly reduced the viability of SAOS-2 cells while significantly increasing the mineralization rate. In vivo, the therapy positively influenced BIC and BIN during the osseointegration phase. Full article

The acoustic performance of windows significantly influences evaluations of building quality, particularly in urban environments. This study presents the results of laboratory tests on the airborne sound insulation of windows with dimensions greater than those specified in ISO 10140-5:2021-10. The aim was to determine the impact of construction details and installation techniques on sound insulation, specifically R_w and R_w + C_tr values. The experimental variables included mounting methods (expansion tape versus low-pressure polyurethane foam), the presence or absence of a threshold in the lower frame, and the type of mullion (fixed versus movable). The tests involved two types of IGUs characterized by different acoustic properties. The findings indicate that the frame configuration, including threshold and mullion type, has a negligible influence on sound insulation. However, the standard method for estimating acoustic performance (EN 14351-1:2006 + A2:2017), which relies on IGU-based data, proved unreliable for modern window assemblies. The estimated values of R_w and R_w + C_tr were consistently lower than those obtained from direct laboratory measurements. These results highlight the need for verification through full-size window testing and suggest that reliance on simplified estimation procedures may lead to underperformance in real-world acoustic applications. Full article

The growing demand for functional and sustainable foods has driven food innovation, enhancing its nutritional value. This study aimed to develop a nutritious bread using local rye from the Trás-os-Montes region of Portugal and incorporating whey, a by-product of the dairy industry, as a replacement for water. Three bread formulations were tested: a traditional recipe with 37.5% rye flour and water (Control—CTR); the same recipe using whey instead of water (Rye Whey—RW); and a formulation with 100% local rye and whey replacing water (Full Rye Whey—FRW). Nutritional composition was assessed, including moisture, ash, protein, dietary fiber, sodium, potassium, lipids, and carbohydrates. Sensory analysis included both quantitative descriptive analysis and consumer acceptance testing. Microbiological quality was also evaluated. Whey-containing samples showed lower moisture and increased levels of ash, lipids, carbohydrates, and potassium. RW had the highest protein content (6.54 ± 0.28 g/100 g, p < 0.05), while FRW exhibited the highest dietary fiber (6.96 ± 0.15 g/100 g, p < 0.05). RW demonstrated a balanced nutritional and sensory profile, with high consumer acceptance. Overall, the combination of local rye and whey presents a promising strategy for producing nutritious bread while valorizing local agricultural resources and dairy by-products. These findings support sustainable food production practices and contribute to circular economy approaches. Full article

This study explores the application of spiking neural networks (SNNs) for click-through rate (CTR) prediction in personalized online advertising systems, introducing a novel hybrid model, the Temporal Rate Spike with Attention Neural Network (TRA–SNN). By leveraging the biological plausibility and energy efficiency of SNNs, combined with attention-based mechanisms, the TRA–SNN model captures temporal dynamics and rate-based patterns to achieve performance comparable to state-of-the-art Artificial Neural Network (ANN)-based models, such as Deep & Cross Network v2 (DCN-V2) and FinalMLP. The models were trained and evaluated on the Avazu and Digix datasets, using standard metrics like AUC-ROC and accuracy. Through rigorous hyperparameter tuning and standardized preprocessing, this study ensures fair comparisons across models, highlighting SNNs’ potential for scalable, sustainable deployment in resource-constrained environments like mobile devices and large-scale ad platforms. This work is the first to apply SNNs to CTR prediction, setting a new benchmark for energy-efficient predictive modeling and opening avenues for future research in hybrid SNN–ANN architectures across domains like finance, healthcare, and autonomous systems. Full article

Maternal obesity programs the fetus for increased risk of chronic disease development in early life and adulthood. We hypothesized that maternal nutrient excess leads to fetal inflammation and impairs offspring skeletal muscle mitochondrial biogenesis in non-human primates. At least 12 months before pregnancy, female baboons were fed a normal chow (CTR, 12% energy fat) or a maternal nutrient excess (MNE, 45% energy fat, and ad libitum fructose sodas) diet, with the latter to induce obesity. After 165 days of gestation (0.9 G), offspring baboons were delivered by cesarean section, and the soleus muscle was collected (CTR n = 16, MNE n = 5). At conception, MNE mothers presented increased body fat and weighed more than controls. The soleus muscle of MNE fetuses exhibited increased levels of stress signaling associated with inflammation (TLR4, TNFα, NF-kB p65, and p38), concomitant with reduced expression of key regulators of mitochondrial biogenesis, including PGC1α, both at the protein and transcript levels, as well as downregulation of PPARGC1B, PPARA, PPARB, CREB1, NOS3, SIRT1, SIRT3. Decreased transcript levels of NRF1 were observed alongside diminished mitochondrial DNA copy number, mitochondrial fusion elements (MFN1, MFN2), cytochrome C protein levels, and cytochrome C oxidase subunits I and II transcripts (cox1 and cox2). MNE coupled to MO-induced stress signaling in fetal baboon soleus muscle is associated with impaired mitochondrial biogenesis and lower mitochondrial content, resembling the changes observed in metabolic dysfunctions, such as diabetes. The observed fetal alterations may have important implications for postnatal development and metabolism, potentially increasing the risk of early-onset metabolic disorders and other non-communicable diseases. Full article

Background/Objectives: This prospective intervention study examined the learning effect of using 3D-printed phantom heads with cleft lip and palate (CLP) and upper jaw models with CLP and maxillary plates during a lecture for dental students in their fourth year at J. W. Goethe Frankfurt University. The primary aim was to evaluate the impact of 3D-printed models on students’ satisfaction levels along with their understanding and knowledge in dental education. Methods: Six life-sized phantom heads with removable mandibles (three with unilateral and three with bilateral CLP) were designed using ZBrush software (Pixologic Inc., Los Angeles, CA, USA) based on MRI images and printed with an Asiga Pro 4K 3D printer (Asiga, Sydney, Australia). Two groups of students (n = 81) participated in this study: the control (CTR) group (n = 39) attended a standard lecture on cleft lip and palate, while the intervention (INT) group (n = 42) participated in a hands-on seminar with the same theoretical content, supplemented by 3D-printed models. Before and after the session, students completed self-assessment questionnaires and a multiple-choice test to evaluate knowledge improvement. Data analysis was conducted using the chi-square test for individual questions and the Wilcoxon rank test for knowledge gain, with the significance level set at 0.05. Results: The study demonstrated a significant knowledge increase in both groups following the lecture (p < 0.001). Similarly, there were significant differences in students’ self-assessments before and after the session (p < 0.001). The knowledge gain in the INT group regarding the anatomical features of unilateral cleft lip and palate was significantly higher compared to that in the CTR group (p < 0.05). Conclusions: The results of this study demonstrate the measurable added value of using 3D-printed models in dental education, particularly in enhancing students’ understanding of the anatomy of cleft lip and palate. Full article

Konjac glucomannan (KGM), derived from Amorphophallus konjac, is increasingly utilized in food and pharmaceutical applications. However, inconsistent KGM production across cultivars jeopardizes its quality and market viability. Lanthanum (La) has been shown to promote KGM levels, but the underlying mechanism remains unclear. In this study, 20~80 mg L⁻¹ La significantly stimulated KGM accumulation compared with the control group. We performed a transcriptome analysis and found 21,047 differentially expressed genes (DEGs), predominantly enriched in carbohydrate and glycan metabolism pathways. A total of 48 DEGs were linked to KGM biosynthesis, with 20 genes (SuSy, INV1/3/5/6, HK1/2, FPK2, GPI3, PGM3, UGP2, GMPP1/4, CslA3~7, CslH2, and MSR1.2) showing significant positive correlations with KGM content. Interestingly, three key terminal pathway genes (UGP1, UGP3, and CslD3) exhibited strong upregulation (log2 fold change > 3). Seven DEGs were validated with qRT-PCR, aligning with the transcriptomic results. Furthermore, 12 hormone-responsive DEGs, including 4 ethylene-related genes (CTR1, EBF1/2, EIN3, and MPK6), 6 auxin-related genes (AUX/IAA1-3, SAUR1-2, and TIR1), and 2 gibberellin-related genes (DELLA1-2), were closely linked to KGM levels. Additionally, the transcription factors bHLH and AP2/ERF showed to be closely related to the biosynthesis of KGM. These results lay the foundation for a model wherein La (Ш) modulates KGM accumulation by coordinately regulating biosynthetic and hormonal pathways via specific transcription factors. Full article

Background: Epiretinal membrane (ERM) and proliferative diabetic retinopathy (PDR) belong to the group of vitreoretinal diseases, characterized by impairments at both the retina and the vitreous. The non-diabetic and diabetic forms of ERM (no-dERM and dERM) as well as the PDR are caused by microvascular disorder, which frequently occurs in association with inflammation and oxidative stress. To better characterize no-dERM, dERM, and PDR at the biomolecular level, we compared the expression of inflammatory, oxidative, lipidic peroxidation products, and complement receptors. Methods: Twenty-seven ocular fluids from patients who underwent phaco-vitrectomy were categorized as no-dERM (9, 4M/5F; 70.4 ± 6.4), dERM (6, 3M/3F; 73.2 ± 4.9), and PDR (6, 5M/1F; 63.7 ± 7.4). Six cataracts (CTR; 3M/3F; 77.7 ± 9.0) were collected for internal control of aqueous cells. Results: In aqueous cells, p65NFkB, iNOS, Nox1/Nox4, and Nrf2 were significantly upregulated, and Keap1 was downregulated in dERM compared with PDR and no-dERM. In aqueous cells, a significant upregulation for C3aR1mRNA, C5aR1mRNA, and CFHmRNA were observed in dERM. In vitreous, C3a, C5b9, and MDA levels were significantly increased in dERM compared with PDR and no-dERM. Conclusions: Inflammatory and ROS products, as well as C3aR1/C5aR1 and soluble MDA, appear of great interest, as their expression in aqueous and vitreous might have potential prognostic and therapeutic values. Full article

Deterministic random bit generators (DRBG) play a crucial role in device security because they generate secret information cryptographic systems, e.g., secret keys and parameters. Thus, attacks on DRBGs can result in the exposure of important secret values, which can threaten the entire cryptographic system of the target Internet of Things (IoT) equipment and smart devices. In 2020, Meyer proposed a side-channel attack (SCA) method that recovers the output random bits by analyzing the power consumption traces of the NIST standard AES CTR DRBG. In addition, most algorithmic countermeasures against SCAs also utilize random numbers; thus, such vulnerabilities are more critical than other SCAs on cryptographic modules. Meyer’s attack recovers the secret random number in four stages of the attack using only the power traces, which the CTR DRBG processes in 256 blocks. We present an approach that employs a clustering algorithm to enhance Meyer’s attack. The proposed attack increases the attack success rate and recovers more information using a clustering attack in the first step. In addition, it improves the attack accuracy in the third and fourth steps using the information obtained from the clustering process. These results lead to the possibility of attacks at higher noise levels and increase the diversity of target devices for attacking the CTR DRBG. Experiments were conducted on an Atmel XMEGA128D4 processor to evaluate the effectiveness of the proposed attack method. We also introduced artificial noise into the power traces to compare the proposed attack’s performance at different noise levels. Our results demonstrate that the first step of the proposed attack achieves a higher success rate than Meyer’s attack at all noise levels. For example, at high noise levels, the difference in the success rates is up to 50%. In steps 3 and 4, an average performance improvement of 18.5% greater than Meyer’s proposed method is obtained. The proposed attack effectively extends the target to more noisy environments than previous attacks, thereby increasing the threat of SCA on CTR DRBGs. Full article

Vermipsyllid parasitize the body surface of sheep, feeding on blood and transmitting diseases, causing severe economic losses to the livestock industry. An outbreak of sheep Vermipsyllid fleas in the mountain pastures of Xinjiang’s Altai region showed that several commonly used commercial anthelmintic drugs had poor therapeutic effects on the prevalent flea species. This study first conducted species identification of Vermipsyllid through morphological analysis of 200 female and 40 male specimens collected from the Altai region, followed by molecular biological identification of 6 randomly selected fleas (3 females and 3 males). Finally, pharmacodynamic experiments were performed to screen for highly effective anthelmintic drugs. Ninety Altai multiparous ewes infected with Vermipsyllid were divided into six groups (fifteen sheep per group): an untreated control group (Ctr), avermectin injection group (Group I), ivermectin injection group (Group II), moxidectin pour-on group (Group III), cypermethrin pour-on group (Group IV), and dichlorvos pour-on group (Group V), with a 14-day trial period. On Day 0, each group received a single treatment according to drug instructions and specified dosages. The number of fleas, flea population reduction rates, and cure rates were measured on Day 0, Day 3, Day 7, and Day 14 to screen for effective anthelmintic drugs. Results showed that unfed female and male fleas were grayish-brown. Engorged females reached 14.15 mm in size, appearing pale white or yellow, with their sterna maintaining the original size and shape despite abdominal distension. Female fleas had 20–21 segments on the labium, while males had 16–19 segments. The hind tibiae featured four notches, and each side of the tergite had 13 ± 1 cup-like indentations. Molecular biological identification indicated that the identified fleas belonged to D. ioffi (Vermipsyllidae, Dorcadia Ioff), showing 99.13% sequence similarity with D. ioffi from Xinjiang, China. In pharmacodynamic experiments, the number of D. ioffi in Groups IV and V decreased to zero on both Day 7 and Day 14, which was extremely significantly lower than other groups (p < 0.01). The flea population reduction rates in Groups IV and V reached 100% on both days. By Day 14, the cure rates of Groups I, II, and III were 0%, while those of Groups IV and V were 100%. Avermectin injection, ivermectin injection, and moxidectin pour-on showed poor anthelmintic effects, whereas cypermethrin and dichlorvos pour-on exhibited high anthelmintic activity against this flea species. Full article

The increasing threat of quantum computing to traditional cryptographic systems has prompted intense research into post-quantum schemes. Despite SNOVA’s potential for lightweight and secure digital signatures, its performance on embedded devices (e.g., ARMv8 platforms) remains underexplored. This research addresses this gap by presenting the optimal SNOVA implementations on embedded devices. This paper presents a performance-optimized implementation of the SNOVA post-quantum digital signature scheme on ARMv8 processors. SNOVA is a multivariate cryptographic algorithm under consideration in the NIST’s additional signature standardization. Our work targets the performance bottlenecks in the SNOVA scheme. Specifically, we employ matrix arithmetic over ${\mathbb{GF}}_{16}$ and AES-CTR-based pseudorandom number generation by exploiting the NEON SIMD extension and tailoring the computations to the matrix rank. At a low level, we develop rank-specific SIMD kernels for addition and multiplication. Rank 4 matrices (i.e., 16 bytes) are handled using fully vectorized instructions that align with 128-bit-wise registers, while rank 2 matrices (i.e., 4 bytes) are processed in batches of four to ensure full SIMD occupancy. At the high level, core routines such as key generation and signature evaluation are structurally refactored to provide aligned memory layouts for batched execution. This joint optimization across algorithmic layers reduces the overhead and enables seamless hardware acceleration. The resulting implementation supports 12 SNOVA parameter sets and demonstrates substantial efficiency improvements compared to the reference baseline. These results highlight that fine-grained SIMD adaptation is essential for the efficient deployment of multivariate cryptography on modern embedded platforms. Full article

This study evaluated the therapeutic potential of 70% ethanol extracts from six medicinal plants (Chenopodium album, Cassia tora, Cudrania tricuspidata, Dioscorea polystachya, Lonicera japonica, Solidago virgaurea subsp. gigantea) through their antibacterial, antioxidant, cytotoxic, and immunomodulatory activities, targeting applications in aquaculture. All extracts exhibited potent antibacterial activity (MIC ≤ 10 μg/mL) against Aeromonas spp. and Photobacterium damselae subsp. damselae, but limited efficacy against Streptococcus parauberis. C. tricuspidata (CTR) and C. tora (CTO) demonstrated superior antioxidant activity (IC₅₀ = 1292 μg/mL and IC₅₀ = 227 μg/mL, respectively), correlating with high polyphenol content (1498 and 1409 mg GAE/g). CTR displayed significant concentration-dependent cytotoxicity (IC₅₀ = 904.2 μg/mL), while C. album (CA) promoted cell proliferation (132.3% viability). In LPS-stimulated CHSE-214 cells, D. polystachya (DP) induced the highest IL-8 expression (207-fold), followed by Chenopodium album (CA) (194-fold IL-8, 49-fold TNF-α) and CTR (245-fold RIPK2), activating NF-κB, MAPK, and NOD-like receptor pathways critical for teleost immunity. Lonicera japonica (LJ) suppressed TNF-α (0.4-fold) and IRF1 (0.3-fold), indicating anti-inflammatory potential, while S. virgaurea subsp. gigantea (SV) showed biphasic TNF-α modulation (79-fold at 10 μg/mL, 5-fold at 100 μg/mL). These diverse bioactivities, particularly the robust immunomodulatory effects, highlight the promise of these extracts as natural therapeutic agents for fish health management in aquaculture. Full article

Melatonin (MT) has been reported to alleviate chilling injury (CI) in postharvest tomato fruit during low-temperature storage. In the present study, the DNA methylation profile changes in the CpG islands of ethylene signaling genes regulated by MT in postharvest tomato fruit during low-temperature storage were detected. The MT treatment increased the content of total soluble solids (TSS) and enhanced the ethylene production of tomato fruit. Moreover, it decreased titratable acidity (TA) content, inhibited the activity of polygalacturonase (PG), and kept the firmness of tomato fruit under low-temperature storage. In the MT-treated tomato fruit, significant changes in DNA methylation of CpG island of SlACS10, LeCTR1, LeEIN3, SlERF-A1, and LeERT10 genes were induced; the expression of LeCTR1 was inhibited; and the expression of SlACS10, LeEIN3, and SlERF-A1 genes was increased, by which the ethylene signaling might be influenced and the CI was alleviated. The present results provide evidence that the CI of postharvest tomato fruit alleviated by MT might be related to the changes in DNA methylation of ethylene-signaling genes. Full article

Plastic film mulching is widely used in protected agriculture. However, the residues of various types of plastic films, as a consequence, severely affect soil quality. The most widely promoted alternative strategy is the use of biodegradable plastic films. Nevertheless, the research on the effects of different types of plastic films on soil properties remains insufficient. This study explored the impacts of different plastic film mulching on the physicochemical properties and microbial communities of soils for pepper cultivation, with three treatments: traditional polyethylene film (PE-Ctr), PBAT biodegradable film (PBAT bio), and reinforced polyethylene film (RPE). The results showed that the soil pH value was the highest in PE-Ctr treatment, and the soil organic matter content was higher in the biodegradable film treatment, while the electrical conductivity (EC), nitrate, and some cations (Ca²⁺, Mg²⁺) were higher in the RPE treatment. The contents of available trace element Zn, Fe, and Mn increased in the PBAT bio treatment. The bacterial richness and evenness indices were higher in PBAT bio treatment than those of other treatments. The fungal community had a relatively high richness, but a lower evenness, compared to the PE-Ctr and PBAT bio treatments. The use of different plastic films significantly affected the composition of soil bacteria, while differences in the composition of soil fungi were only observed between the PBAT bio and RPE treatments. Proteobacteria, Acidobacteriota, and Actinobacteriota were the most dominant bacterial phyla, and Ascomycota and Mortierellomycota were the dominant fungal phylum across all treatments. FAPROTAX functional prediction showed that the abundances of multiple functions of soil bacteria were higher in the RPE treatment, and the chemoheterotrophy function was higher in the PE treatment. FUNGuild analysis indicated that the trophic types and ecological function groups of soil fungi were more abundant in the PBAT bio treatment. Full article