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Dentistry Journal
Volume 13
Issue 8
10.3390/dj13080351
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Behavior of Osteoblastic Lineage Cells When in the Presence of Tamoxifen: In Vitro and In Vivo Studies on Osseointegration

by
Luiz Guilherme Fiorin
1,2,
Emanuela Galliera
3,4,
Henrique R. Matheus
1,5,
Dolaji Henin
2,
Edilson Ervolino
6,
Gabriela Carrara Simionato
1,
Juliano Milanezi de Almeida
1,* and
Claudia Dellavia
2
1
Department of Diagnosis and Surgery, Division of Periodontics, São Paulo State University “Júlio de Mesquita Filho”—UNESP, Araçatuba 16015-050, Brazil
2
Thin Section Laboratory, Department of Biomedical, Surgical and Dental Sciences, Università degli Studi di Milano Statale (UNIMI), 7-20122 Milan, Italy
3
Department of Biomedical Sciences for Health, Università degli Studi di Milano, 7-20122 Milan, Italy
4
Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS)—Istituto Ortopedico Galeazzi, 20157 Milan, Italy
5
Discipline of Periodontics, School of Dentistry, University of São Paulo, São Paulo 05508-000, Brazil
6
Department of Basic Science, São Paulo State University (UNESP), School of Dentistry, Araçatuba 16018-805, Brazil
*
Author to whom correspondence should be addressed.
Dent. J. 2025, 13(8), 351; https://doi.org/10.3390/dj13080351 (registering DOI)
Submission received: 27 February 2025 / Revised: 17 July 2025 / Accepted: 25 July 2025 / Published: 1 August 2025
Versions Notes

Abstract

Background/Objectives: Tamoxifen, a selective estrogen receptor modulator widely used as an adjunct in the treatment of breast cancer, has known effects on bone metabolism, although its impact on osseointegration and cellular responses during early bone healing remains unclear. Understanding these effects is essential given the increasing use of dental implants in cancer survivors. The study aimed to observe the influence of tamoxifen on human osteosarcoma (SAOS-2) cells lines, as well on the osseointegration of titanium implants in ovariectomized female rats. Methods: SAOS-2 cells were incubated with Dulbecco’s modified growth medium. Six titanium (Ti) disks were used at each time point. The samples were divided into groups with the presence (TAM, n = 36) or not (CTR, n = 36) of tamoxifen in a concentration of 2 μM. In vivo, 72 animals were divided in groups with bilateral ovariectomy or SHAM and tamoxifen administration or not (15 mg/kg). Cell viability, mineralization rate, and collagen synthesis were assessed, as well as bone/implant contact (BIC) and bone ingrowth (BIN). Results: Tamoxifen caused a decrease in SAOS-2 viability, although an increase in the mineralization rate was observed. In vivo, the TAM groups presented higher BIC and BIN when compared to their control, but a lower percentage of mature collagen cells. Conclusions: Based on our findings, in vitro, the therapy with TAM slightly reduced the viability of SAOS-2 cells while significantly increasing the mineralization rate. In vivo, the therapy positively influenced BIC and BIN during the osseointegration phase.
Keywords: tamoxifen; selective estrogen receptor modulators; cell culture; titanium implants tamoxifen; selective estrogen receptor modulators; cell culture; titanium implants

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MDPI and ACS Style

Fiorin, L.G.; Galliera, E.; Matheus, H.R.; Henin, D.; Ervolino, E.; Simionato, G.C.; de Almeida, J.M.; Dellavia, C. Behavior of Osteoblastic Lineage Cells When in the Presence of Tamoxifen: In Vitro and In Vivo Studies on Osseointegration. Dent. J. 2025, 13, 351. https://doi.org/10.3390/dj13080351

AMA Style

Fiorin LG, Galliera E, Matheus HR, Henin D, Ervolino E, Simionato GC, de Almeida JM, Dellavia C. Behavior of Osteoblastic Lineage Cells When in the Presence of Tamoxifen: In Vitro and In Vivo Studies on Osseointegration. Dentistry Journal. 2025; 13(8):351. https://doi.org/10.3390/dj13080351

Chicago/Turabian Style

Fiorin, Luiz Guilherme, Emanuela Galliera, Henrique R. Matheus, Dolaji Henin, Edilson Ervolino, Gabriela Carrara Simionato, Juliano Milanezi de Almeida, and Claudia Dellavia. 2025. "Behavior of Osteoblastic Lineage Cells When in the Presence of Tamoxifen: In Vitro and In Vivo Studies on Osseointegration" Dentistry Journal 13, no. 8: 351. https://doi.org/10.3390/dj13080351

APA Style

Fiorin, L. G., Galliera, E., Matheus, H. R., Henin, D., Ervolino, E., Simionato, G. C., de Almeida, J. M., & Dellavia, C. (2025). Behavior of Osteoblastic Lineage Cells When in the Presence of Tamoxifen: In Vitro and In Vivo Studies on Osseointegration. Dentistry Journal, 13(8), 351. https://doi.org/10.3390/dj13080351

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