Search Results (484)

Background and Objectives: This study aimed to evaluate the clinical effectiveness and safety of ultrasound-guided pharmacopuncture (UGP) in comparison to non-guided pharmacopuncture (NGP) for the treatment of acute cervical myofascial pain syndrome (C-MPS) in primary care settings. Materials and Methods: This multi-center, prospective observational study included 97 patients diagnosed with acute C-MPS. Participants received a single session of either UGP or NGP at one of seven primary care institutions. Pain intensity was measured using the Numerical Rating Scale (NRS), and cervical function was assessed through active Range of Motion (ROM) tests conducted before and after treatment. We conducted follow-up interviews within 48 h after treatment to monitor adverse events. Results: Both groups showed significant improvements in pain levels and cervical ROM after treatment. The UGP group showed a greater reduction in NRS scores compared to the NGP group (p < 0.001). Notable enhancements in cervical extension and rotation on the affected side were also observed in the UGP group (p < 0.01), whereas changes in flexion and lateral flexion were similar between the two groups. No serious adverse events were reported. Conclusions: UGP has shown superior pain reduction and a greater improvement in specific cervical motions compared to non-guided treatments, indicating enhanced precision and therapeutic efficacy. Furthermore, no serious adverse events were reported, suggesting that UGP is a safe and effective non-surgical intervention for acute C-MPS in real-world primary care settings. Full article

The aim of this study was to evaluate the agronomic performance and physicochemical characteristics of broccoli grown under different doses and sources of special phosphorus (P) fertilizers and their residual effect on the soil, in Cerrado mineiro. A randomized block design arranged in a split-plot scheme was employed, where three P sources—T1 = Conventional monoammonium phosphate (CMP); T2 = Polymerized monoammonium phosphate (PCMP); T3 = Granulated organomineral fertilizer (GOF)—along with four P₂O₅ rates—1–0 (No P); 2–50% (200 kg ha⁻¹ P₂O₅); 3–75% (300 kg ha⁻¹ P₂O₅); and 4–100% (400 kg ha⁻¹ P₂O₅)—were assessed. Evaluations included the number of leaves (NL), head fresh (HFM) and dry mass (HDM), yield (YLD), soil fertility at harvest, plant nutritional status, and the physicochemical quality of the harvested broccoli. It was observed that GOF provided the best agronomic performance (HFM, HDM and YLD) of the broccoli and the greatest residual effect in the soil compared to PCMP and CMP. The moisture, ash, protein, lipid, total titratable acid and ascorbic acid contents were not significantly (p < 0.05) affected by the fertilizers used, on the other hand, total soluble solids and hydrogen potential showed the highest and lowest values, respectively, with CMP. The best agronomic performance, the highest phosphorus content in the soil and plant and the best physical–chemical quality of the broccoli occurred at a dose of 100% (400 kg ha⁻¹ of P₂O₅) of the recommendation for the crop in all three fertilizers evaluated. Full article

Intestinal inflammation involves barrier impairment, immune hyperactivation, and oxidative stress imbalance. Bioactive polysaccharides universally alleviate inflammation via anti-inflammatory, antioxidant, and microbiota-modulating effects, yet exhibit distinct core mechanisms. Elucidating these differences is vital for targeted polysaccharide applications. This research examines distinct regulatory pathways through which diverse bioactive polysaccharides mitigate lipopolysaccharide-triggered intestinal inflammation in male Kunming (KM) mice. This experiment employed Lentinula edodes polysaccharide (LNT), Auricularia auricula polysaccharide (AAP), Cordyceps militaris polysaccharide (CMP), Lycium barbarum polysaccharide (LBP), and Brassica rapa polysaccharide (BRP). The expression levels of biomarkers associated with the TLR4 signaling pathway, oxidative stress, and intestinal barrier function were quantified, along with comprehensive gut microbiota profiling. The results showed that all five polysaccharides alleviated inflammatory responses in mice by inhibiting inflammatory cytokine release, reducing oxidative damage, and modulating gut microbiota, but their modes of action differed: LBP significantly suppressed the TLR-4/MyD88 signaling pathway and its downstream pro-inflammatory cytokine expression, thereby blocking inflammatory signal transduction and reducing oxidative damage; LNT and CMP enhanced the body’s antioxidant capacity by increasing antioxidant enzyme activities and decreasing malondialdehyde (MDA) levels; AAP and BRP enriched Akkermansia (Akk.) within the Verrucomicrobia (Ver.) phylum, upregulating tight junction protein expression to strengthen the intestinal mucosal barrier and indirectly reduce oxidative damage. This research demonstrates that different polysaccharides alleviate inflammation through multi-target synergistic mechanisms: LBP primarily inhibits inflammatory pathways; AAP and BRP focus on intestinal barrier protection and microbiota modulation; and LNT and CMP exert effects via antioxidant enzyme activation. These data support designing polysaccharide blends that leverage complementary inflammatory modulation mechanisms. Full article

The Qiangtang Basin (Tibetan Plateau) poses significant geophysical challenges for seismic exploration due to near-surface widespread permafrost and steeply dipping Mesozoic strata induced by the Cenozoic Indo-Eurasian collision. These seismic geological conditions considerably contribute to lower signal-to-noise ratios (SNRs) with complex wavefields, to some extent reducing the reliability of conventional seismic imaging and structural interpretation. To address this, the common-reflection-surface (CRS) stack method, derived from optical paraxial ray theory, is implemented to transcend horizontal layer model constraints, offering substantial improvements in high-SNR prestack gather generation and prestack depth migration (PSDM) imaging, notably for permafrost zones. Using 2D seismic data from the basin, we detailedly compare the CRS stack with conventional SNR enhancement techniques—common midpoint (CMP) FlexBinning, prestack random noise attenuation (PreRNA), and dip moveout (DMO)—evaluating both theoretical foundations and practical performance. The result reveals that CRS-processed prestack gathers yield superior SNR optimization and signal preservation, enabling more robust PSDM velocity model building, while comparative imaging demonstrates enhanced diffraction energy—particularly at medium (20–40%) and long (40–60%) offsets—critical for resolving faults and stratigraphic discontinuities in PSDM. This integrated validation establishes CRS stacking as an effective preprocessing foundation for the depth-domain imaging of complex permafrost geology, providing critical improvements in seismic structural resolution and reduced interpretation uncertainty for hydrocarbon exploration in permafrost-bearing basins. Full article

This article aims to offer a broad perspective on the use of non-invasive brain stimulation (NIBS) techniques in the context of chronic musculoskeletal pain (CMP) conditions. While NIBS has demonstrated promising efficacy in certain chronic pain populations, its application in the management of CMP remains limited. This paper examines the current evidence supporting the use of NIBS for pain relief in CMP, the rationale and proposed mechanisms of action, the importance of patient selection, common methodological limitations in the existing literature, and the potential adverse effects of these techniques. The authors argue that the current evidence is insufficient to support widespread clinical adoption of NIBS for CMP. Advancing the field will require more rigorous study designs, with adequately powered and properly blinded randomized controlled trials. Additionally, future research should address the identification of potential responders to brain stimulation, conduct economic evaluations, and carefully assess the benefit–risk ratio before NIBS can be integrated into routine clinical practice. Full article

To address the issue of surface glazing that occurs during prolonged polishing with gel tools, this study employs a triethanolamine (TEA)-based polishing fluid system to enhance the self-sharpening capability of the gel polishing disc. The inhibitory mechanism of TEA concentration on disc glazing is systematically analyzed, along with its impact on the gel disc’s frictional wear behaviour. Furthermore, the synergistic effects of process parameters on both surface quality and material removal rate (MRR) of SiC are examined. The results demonstrate that TEA concentration is a critical factor in regulating polishing performance. At an optimal concentration of 4 wt%, an ideal balance between chemical chelation and mechanical wear is achieved, effectively preventing glazing while avoiding excessive tool wear, thereby ensuring sustained self-sharpening capability and process stability. Through orthogonal experiment optimization, the best parameter combination for SiC polishing is determined: 4 wt% TEA concentration, 98 N polishing pressure, and 90 rpm rotational speed. This configuration delivers both superior surface quality and desirable MRR. Experimental data confirm that TEA significantly enhances the self-sharpening performance of gel discs through its unique complex reaction. During the rough polishing stage, the MRR increases by 34.9% to 0.85 μm/h, while the surface roughness S_a is reduced by 51.3% to 6.29 nm. After subsequent CMP fine polishing, an ultra-smooth surface with a final roughness of 2.33 nm is achieved. Full article

Hybrid materials based on porous organic polymers (POPs) and metal–organic frameworks (MOFs) are increasing attention for advanced separation processes due to the possibility to combine their properties. POPs provide high surface areas, chemical stability, and tunable porosity, while MOFs contribute a high variety of defined crystalline structures and enhanced separation characteristics. The combination (or hybridization) with PIMs gives rise to mixed-matrix membranes (MMMs) with improved permeability, selectivity, and long-term stability. However, interfacial compatibility remains a key limitation, often addressed through polymer functionalization or controlled dispersion of the MOF phase. MOF/COF hybrids are more used as biochemical sensors with elevated sensitivity, catalytic applications, and wastewater remediation. They are also very well known in the gas sorption and separation field, due to their tunable porosity and high electrical conductivity, which also makes them feasible for energy storage applications. Last but not less important, hybrids with other POPs, such as hyper-crosslinked polymers (HCPs), covalent triazine frameworks (CTFs), or conjugated microporous polymers (CMPs), offer enhanced functionality. MOF/HCP hybrids combine ease of synthesis and chemical robustness with tunable porosity. MOF/CTF hybrids provide superior thermal and chemical stability under harsh conditions, while MOF/CMP hybrids introduce π-conjugation for enhanced conductivity and photocatalytic activity. These and other findings confirm the potential of MOF-POP hybrids as next-generation materials for gas separation and carbon capture applications. Full article

Wafer cleaning after chemical mechanical planarization (CMP) is a critical processing step for copper metallization in integrated circuits. Post-CMP cleaning (PCMPC) commonly combines surface (electro)chemistry with the tribology of brush scrubbing to remove CMP residues from wafer surfaces. While the complex mechanisms of brush-operated PCMPC are supported by this combination, the conventional electroanalytical methods of assessing PCMPC efficiency are typically operated in the absence of surface brushing. Using a model experimental system with tartaric acid (TA) as a cost-effective cleaner of Cu-oxides, we illustrate here how post-CMP Cu samples can be electrochemically examined using brush cleaning to design/assess PCMPC test solutions. A pH-neutral cleaning solution is employed, where TA also serves as a partial dissolution suppressor of Cu, and CMP-treated wafer samples are scrubbed with a commercial PCMPC brush as sample surfaces are simultaneously probed with electrochemical measurements. The results show the active roles of tribology/lubrication and surface chemistry in the removal of CMP residues. The electrochemically determined residue removal efficiencies of PCMPC are found to be ~97% and ~56% in the presence and in the absence of surface brushing, respectively. The implications of these findings are explored in the general context of evaluating PCMPC formulations. Full article

Sudden cardiac death represents an unexpected death for which a strong underlying genetic background has been described. The primary causes are identified in cardiomyopathies and channelopathies, which are heart diseases of the muscle and electrical system, respectively, without coronary artery disease, hypertension, valvular disease, and congenital heart malformations. Genetic variants, especially single nucleotide variants and short insertions/deletions impacting essential myocardial functions, have shown that cardiomyopathies display high heritability. However, genetic heterogeneity, incomplete penetrance, and variable expression may complicate the interpretation of genetic findings, thus delaying the management of seriously at-risk patients. Moreover, recent studies show that the diagnostic yield related to genetic cardiomyopathies ranges from 28 to 40%, raising the need for further research. In this regard, investigating the occurrence of structural variants, especially copy number variants, may be crucial. Based on these considerations, this review aims to provide an overview of copy number variants identified in cardiomyopathies and discuss them, considering diagnostic yield. This review will ultimately address the necessity of incorporating copy number variants into routine genetic testing for cardiomyopathies and channelopathies, a process increasingly enabled by advances in next-generation sequencing technologies. Full article

High-Bandwidth Memory (HBM) enables the bandwidth required by modern AI and high-performance computing, yet its three dimensional stack traps heat and amplifies thermo mechanical stress. We first review how conventional solutions such as heat spreaders, microchannels, high density Through-Silicon Vias (TSVs), and Mass Reflow Molded Underfill (MR MUF) underfills lower but do not eliminate the internal thermal resistance that rises sharply beyond 12layer stacks. We then synthesize recent hybrid bonding studies, showing that an optimized Cu pad density, interface characteristic, and mechanical treatments can cut junction-to-junction thermal resistance by between 22.8% and 47%, raise vertical thermal conductivity by up to three times, and shrink the stack height by more than 15%. A meta-analysis identifies design thresholds such as at least 20% Cu coverage that balances heat flow, interfacial stress, and reliability. The review next traces the chain from Coefficient of Thermal Expansion (CTE) mismatch to Cu protrusion, delamination, and warpage and classifies mitigation strategies into (i) material selection including SiCN dielectrics, nano twinned Cu, and polymer composites, (ii) process technologies such as sub-200 °C plasma-activated bonding and Chemical Mechanical Polishing (CMP) anneal co-optimization, and (iii) the structural design, including staggered stack and filleted corners. Integrating these levers suppresses stress hotspots and extends fatigue life in more than 16layer stacks. Finally, we outline a research roadmap combining a multiscale simulation with high layer prototyping to co-optimize thermal, mechanical, and electrical metrics for next-generation 20-layer HBM. Full article

Nowadays, healthcare systems face two global challenges: the rise of multidrug-resistant pathogens and the growing incidence of cancer. Due to their broad spectrum of activities, antimicrobial peptides emerged as potential alternatives against both threats. Our group previously described the antifungal activity of the α-helical peptide Cm-p5, a derivative of the natural peptide Cm-p1, isolated from the coastal mollusk Cenchritis muricatus; however, its anti-cancer properties remained unexplored. Analyses through calorimetry and molecular dynamics simulations suggest the relevance of phosphatidylserine for the attachment of Cm-p5 to cancer cell membranes. Cm-p5 exhibited cytotoxic activity in a dose-dependent manner against A375 melanoma cells, without toxicity against non-malignant cells or hemolytic activity. DAPI/PI and DiSC3(5) staining confirmed permeabilization, disruption, and depolarization of A375 cytoplasmic membranes by Cm-p5. Furthermore, Annexin V-FITC/PI assay revealed the induction of cellular death in melanoma cells, which can result from the cumulative membrane damage and oxidative stress due to the overproduction of reactive oxygen species (ROS). Moreover, after the treatment, the proliferation of A375 cells was dampened for several days, suggesting that Cm-p5 might inhibit the recurrence of melanomas. These findings highlight the multifunctional nature of Cm-p5 and its potential for treating malignant melanoma. Full article

In this paper, a novel through-silicon via (TSV) fabrication strategy based on through-hole structures is proposed for low-cost and low-complexity manufacturing. Compared to conventional TSV fabrication processes, this method significantly simplifies the process flow by employing double-sided liner deposition, double-sided barrier layer/seed layer formation, and double-sided Cu electroplating. This method enhances the TSV stability by eliminating Cu contamination issues during chemical–mechanical polishing (CMP), which are a common challenge in traditional blind via fabrication processes. Additionally, the liner and barrier layer/seed layer achieve a high step coverage exceeding 80%, ensuring excellent conformality and structural integrity. For electroplating, a multi-stage bi-directional electroplating technique is introduced to enable void-free Cu filling in TSVs. The fabricated TSVs exhibit an ultra-low leakage current of 135 fA at 20 V, demonstrating their potential for advancing 3D integration technologies in heterogeneous integration. Full article

Conjugated microporous polymers (CMP) are advanced photocatalytic systems for degrading organic dyes. However, their potential and efficiency are often limited by rapid electron–hole pair (e⁻/h⁺) recombination. To overcome this limitation, this study proposes a strategy that involves designing a Mott–Schottky heterojunction and integrating graphene sheets with a near-zero bandgap into the CMP-1 framework, resulting in a non-covalent graphene/CMP (GCMP) heterojunction composite. GCMP serves two main functions: physical adsorption and photocatalytic absorption that uses visible light energy to trigger and degrade the organic dye. GCMP effectively degraded four dyes with both anionic and cationic properties (Rhodamine B; Nile Blue; Congo Red; and Orange II), demonstrating stable recyclability without losing its effectiveness. When exposed to visible light, GCMP generates reactive oxygen species (ROS), primarily singlet oxygen (¹O₂), and superoxide radicals (^•O₂), degrading the dye molecules. These findings highlight GCMP’s potential for real-world applications, offering a metal-free, cost-effective, and environmentally friendly solution for wastewater treatment. Full article

To address the challenge of assessing the thickness uniformity of epoxy asphalt layers on steel bridge decks, three-dimensional ground-penetrating radar (3D-GPR) was employed for non-destructive, full cross-sectional detection of the pavement layer’s thickness. The antenna array spacing was optimized using the common midpoint (CMP) method, enabling precise measurement of the relative permittivity of epoxy asphalt mixtures. A significant correlation between relative permittivity and the void ratio was established, providing a novel approach to identifying areas prone to coarse segregation and early-stage water damage. Grayscale maps of the thickness distribution enabled precise detection of regions with acceptable, under-thickness and over-thickness values. The uniformity of construction thickness was quantitatively evaluated using standard deviations and coefficients of variation. Results indicated that when the coefficient exceeds 12%, improvements in the pavement construction process are necessary. This research demonstrates the capability of 3D-GPR to effectively detect thickness variations, offering a valuable tool for enhancing pavement paving and compaction practices on steel bridge decks. Full article

To investigate the interaction effects of resting times (0, 6, and 12 h) and NaHCO₃ concentrations (0, 2, 4, and 6 g/kg) on chicken myofibrillar protein (CMP), this study analyzed the changes in solubility, active sulfhydryl groups, rheological behavior, fluorescence, and gel properties of CMP solutions (60 mg/mL). The results indicated that pH significantly increased with higher NaHCO₃ concentrations and longer resting times. Consequently, solubility, active sulfhydryl groups, apparent viscosity, shear stress, G’ value at 80 °C, hardness, springiness, and cohesiveness all significantly increased, while particle size, turbidity, and whiteness significantly decreased. However, these trends were not observed in samples treated with an amount of 6 g/kg NaHCO₃ and/or a resting time of 12 h. The findings suggest that treatment with 4 g/kg NaHCO₃ and a resting time of 6 h effectively reduced protein aggregation and enhanced solubility. Conversely, excessive NaHCO₃ or prolonged resting times resulted in decreased protein solubility and deteriorated textural properties. Full article