Search Results (307)

This study investigates the effects of copper nanoparticles (CuNPs) on KRT19 gene expression in four breast cancer cell lines (MDA-MB-231, MDA-MB-468, MCF7, and T47D), representing triple-negative and luminal subtypes. Using cytotoxicity assays, quantitative RT-PCR, and bioinformatics tools (STRING, g:Profiler), we demonstrate subtype-specific, dose-dependent KRT19 suppression, with epithelial-like cell lines showing greater sensitivity. CuNPs, characterized by dynamic light scattering (DLS) and transmission electron microscopy (TEM) with a mean size of 179 ± 15 nm, exhibited dose-dependent cytotoxicity. Bioinformatics analyses suggest KRT19′s potential as a biomarker for CuNP-based therapies, pending in vivo and clinical validation. These findings highlight CuNPs’ therapeutic potential and the need for further studies to optimize their application in personalized breast cancer treatment. Full article

Sepsis is a life-threatening condition characterized by dysregulated immune responses to infection. To elucidate early transcriptional changes in sepsis, we conducted a case–control study profiling gene expression in whole blood from 20 early-stage sepsis patients and 9 healthy controls. Using Affymetrix Clariom D Human Arrays and robust preprocessing, we identified differentially expressed genes (DEGs) using standard bioinformatic pipelines. A total of 344 genes were significantly upregulated, while 9703 were significantly downregulated in sepsis patients (|log2FC| > 1, adjusted p < 0.05). Pathway enrichment and Gene Ontology analysis revealed activation of innate immune pathways, neutrophil degranulation, and cytokine signaling, alongside suppression of lymphocyte differentiation and antigen presentation. These results suggest a shift toward an innately driven inflammatory state in early sepsis. Our findings provide transcriptomic insights that may support the development of early diagnostic biomarkers and therapeutic targets. Full article

Background/Objectives: Despite its well-established health benefits, adherence to the Mediterranean lifestyle (MedLife) has declined globally, including in its region of origin, alongside a significant shift toward ultra-processed food consumption. Understanding the factors associated with MedLife adherence is essential for developing targeted interventions and tailored policy recommendations. As part of the MEDIET4ALL PRIMA project, this cross-sectional study aimed to comprehensively examine geo-demographic, socio-economic, psychological, behavioral, and barrier-related factors associated with and potentially contributing to MedLife adherence. Methods: Data were collected from 4010 participants aged 18 years and above across ten Mediterranean and neighboring countries using the multinational MEDIET4ALL e-survey, which included the validated MedLife index, along with various other questionnaires. Results: Results indicate that only 22% of respondents demonstrated high adherence to the Mediterranean lifestyle (MedLife), with significant variability observed across countries, age groups, education levels, and health statuses. Spain had the highest proportion of participants with high adherence (38%). Factors associated with significantly higher adherence rates include older age, living in the Mediterranean region, higher education levels, a greater awareness of MedLife principles, lower perceived barriers, normal BMI, better health status, and stable economic and marital conditions (p-values ranging from 0.04 to <0.001). Additionally, individuals with high MedLife adherence exhibited more socially and physically active lifestyles and experienced less psychological strain (p < 0.001). Regression analyses identified MedLife awareness as the strongest positive predictor of adherence (β = 0.206), followed by social participation (β = 0.194) and physical activity (β = 0.096). Additional positive contributors include life satisfaction, sleep quality, living in the Mediterranean region, age, and education (β ranging from 0.049 to 0.093). Conversely, factors that are negatively associated with adherence include sedentary behavior, living environment, and barriers such as low motivation, taste dislike, price unaffordability, limited availability, and the time-consuming nature of preparing Mediterranean food (MedFood; β ranging from −0.036 to −0.067). Conclusions: These findings indicate that fewer than one in four adults across Mediterranean and neighboring countries demonstrate high adherence to MedLife, supporting prior evidence of suboptimal adherence even within Mediterranean regions. This study identified a range of behavioral, socio-demographic, and environmental factors—both positive and negative predictors—that can help guide the design of targeted, culturally adapted interventions to promote MedLife behavior. Future research should incorporate objective measurements and longitudinal monitoring to better understand underlying mechanisms, establish causality, and develop sustainable strategies for enhancing MedLife adherence in diverse populations. Full article

The Arabian Gulf, bordered by major energy-producing nations, harbors diverse microalgal communities with strong potential for the bioremediation of environmental pollutants, particularly petroleum hydrocarbons. This review evaluates two key microalgal groups—micro-green algae and dinoflagellates—highlighting their distinct physiological traits and ecological roles in pollution mitigation. Dinoflagellates, including Prorocentrum and Protoperidinium, have demonstrated hydrocarbon-degrading abilities but are frequently linked to harmful algal blooms (HABs), marine toxins, and bioluminescence, posing ecological and health risks. The toxins produced by these algae can be hemolytic or neurotoxic and include compounds such as azaspiracids, brevetoxins, ciguatoxins, okadaic acid, saxitoxins, and yessotoxins. In contrast, micro-green algae such as Oedogonium and Pandorina are generally non-toxic, seldom associated with HABs, and typically found in clean freshwater and brackish environments. Some species, like Chlorogonium, indicate pollution tolerance, while Dunaliella has shown promise in remediating contaminated seawater. Both groups exhibit unique enzymatic pathways and metabolic mechanisms for degrading hydrocarbons and remediating heavy metals. Due to their respective phycoremediation capacities and environmental adaptability, these algae offer sustainable, nature-based solutions for pollution control in coastal, estuarine, and inland freshwater systems, particularly in mainland Qatar. This review compares their remediation efficacy, ecological impacts, and practical limitations to support the selection of effective algal candidates for eco-friendly strategies targeting petroleum-contaminated marine environments. Full article

In the vernacular architectural history of the Arabian Gulf region (the Gulf), the courtyard house is a common domestic typology. Islamic and sociological precepts regulate the design and the function of vernacular courtyard dwellings, resulting in homogeneity and harmony in the entire urban fabric of historic settlements. In this research endeavor, the aim is to investigate the degree to which the shaping of the spatial form in a sample of vernacular courtyard houses in the Gulf inscribes socio-cultural factors. It sheds light on visibility graph analysis, human behavior, and the system of activities in the domestic space. As a hypothesis, visibility and connectivity analysis could prove the existence of common spatial patterns among courtyards in the vernacular courtyard houses of the Gulf, attributing it to the similar socio-cultural context, the climatic effect, and the architectural and construction knowledge of the region. This study utilizes a collection of courtyard houses from the Gulf as a pilot study to investigate the emerging patterns in the spatial analysis and room layout, or in the distribution of activities or functions in the domestic space. It, therefore, offers a visual analysis (VGA) of six regional courtyard houses from each Gulf country that were built during the period 1850–1950. This study anticipates an affirmation of a direct inscription of socio-cultural factors in the spatial form of the courtyard house in the Gulf. Conclusively, a sustainable, organic linkage between architecture and society exists where the three criteria of housing spatial form, socio-cultural factors, and system of activities interact. Full article

Sustainable development is increasingly challenged by the growing threats of climate change. There is a close relationship between climate change, public health, and Sustainable Development Goals (SDGs). This study investigates the temperature anomalies in the Kingdom of Bahrain and their potential effects on human health. Furthermore, it proposes solutions to support Bahrain’s SDG-related goals. Data were collected from global studies and statistics and the Bahrain Meteorological Directorate over 50 years, which were then used to calculate the temperature anomalies and the heat indices, thereby exploring the past and present monthly and annual national temperature and sociated risks to human health. The results show that Bahrain is located in an area of high temperature anomalies and high rates of cardiovascular diseases. Furthermore, anomaly calculations indicate a critical rise in temperature, ranging from 1 to 4 °C higher than the averages recorded in the 1960s, 1970s, and 1980s. Such an increase could significantly affect human health, particularly since the heat index results show that summers consistently fall within the extreme danger ranges. In contrast, other seasons have occasionally reached the danger level or required extreme caution in certain years. Consequently, this study offers recommendations to help mitigate the rise in temperature and associated risks in the future. Full article

Sickle cell disease (SCD) is the most common hemoglobinopathy, caused by a mutation in the β-globin gene of hemoglobin. It predisposes patients to painful Vaso-occlusive crises (VOC) and multi-organ dysfunctions. The disease exhibits significant phenotypic variability, making it challenging to predict severity and outcomes. This study aimed to characterize the whole blood gene expression profile of Bahraini SCD patients, identifying differentially expressed genes during steady-state (n = 10) and VOC (n = 10) compared to healthy controls (n = 8). Analysis revealed 2073 and 3363 dysregulated genes during steady-state and VOC, respectively, compared to controls, with 1078 genes differentially expressed during VOC versus steady-state. Gene Ontology (GO) enrichment analysis highlighted significant deregulation in immune and hematopoietic pathways, including down-regulation of critical genes for immune modulation and hematopoietic balance. Notably, the transcription factor RUNX3, involved in immune cell differentiation and inflammation, was among the 668 down-regulated genes. RUNX3 was four-fold down-regulated in microarray analysis, three-fold in PCR, and showed a mean protein concentration of 11.13 pg/mL during VOC compared to 457.93 pg/mL during steady-state (p < 0.01). These findings suggest that RUNX3 may serve as a potential biomarker for VOC. Future large-scale validation, additional proteomic studies, and functional investigations are recommended to confirm its clinical utility and significance. Full article

Mangrove ecosystems are crucial coastal habitats that support life and regulate the Earth’s atmosphere. However, these ecosystems face prominent threats due to anthropogenic activities and environmental constraints. For instance, the Saudi Arabian coast is particularly vulnerable to species extinction and biodiversity loss due to the fragility of the ecosystem; this highlights the need to understand the spatial and temporal dynamics of mangrove forests in desert environments. Hence, this is the first national study to quantify mangrove forests and analyze at-risk zone-based land use along Saudi Arabian coasts over 40 years. Thus, the primary contents of this research were (1) to produce a new long-term dataset covering the entire Saudi coastline, (2) to identify the patterns, analyze the trends, and quantify the change of mangrove areas, and (3) to determine vulnerability zoning of mangrove area-based land use and transportation networks. This study used Landsat satellite imagery via Google Earth Engine for national-scale mangrove mapping of Saudi Arabia between 1985 and 2024. Visible and infrared bands and seven spectral indices were employed as input features for the random forest classifier. The two classes used were mangrove and non-mangrove; the latter class included non-mangrove land-use and land-cover areas. Then, the study employed the output mangrove mapping to delineate vulnerable mangrove forest-based land use. The overall results showed a substantial increase in mangrove areas, ranging from 27.74 to 59.31 km² in the Red Sea and from 1.05 to 8.65 km² in the Arabian Gulf between 1985 and 2024, respectively. However, within this decadal trend, there were noticeable periods of decline. The spatial coverage of mangroves was larger on Saudi Arabia’s western coasts, especially the southwestern coasts, than on its eastern coasts. The overall accuracy, conducted annually, ranged between 91.00% and 98.50%. The results also show that expanding land uses and transportation networks within at-risk zones of mangrove forests may have a high potential effect. This study aimed to benefit the government, conservation agencies, coastal planners, and policymakers concerned with the preservation of mangrove habitats. Full article

Mesenchymal stem cell-derived exosomes (MSC-Exos) play a key role in tissue repair, immune regulation, and cancer biology. Due to limitations in MSC expansion and source variability, interest has shifted to induced pluripotent stem cell-derived MSCs (iMSCs) as a promising alternative. This study compares effects of exosomes derived from iMSCs (iMSC-Exos) and Wharton’s jelly MSCs (WJMSC-Exos) on MCF7 and A549 cancer cells. Both types of exosomes reduced MCF7 proliferation and induced a senescence-like state, rather than apoptosis, although the antiproliferative effect was transient in A549 cells. Notably, WJMSC-Exos promoted migration in both MCF7 and A549, whereas iMSC-Exos did not exhibit this effect. Overall, WJMSC-Exos had a more robust impact on cancer cell proliferation and migration. These findings highlight the diverse effects of exosomes on cancer and the development of a senescence-like state as an important response to Exos exposure. Moreover, these findings invite for more careful evaluation of the therapeutic role of iMSC-derived Exos. Full article

The increase in temperature in the built environment impedes the utilization of outdoor amenities and non-motorized transportation by residents of Arabian Gulf cities throughout the prolonged hot season. The urban heat island (UHI) phenomenon, denoted by the substantial temperature difference between the city and its periphery, is associated with multiple parameters. Building heights, setbacks, and configurations influence the temperature within street canyons. Nowadays, it is vital for urban designers to understand the role of these parameters in UHI effect, and translate those insights into design guidelines and urban forms they propose. This study delves into the relationship between building geometry and urban heat island effects in the context of Doha City, using residential building areas as the basis for comparison. Using dual-pronged methodology, the study entails simulating the dry bulb temperature and the sky view factor, alongside field measurements for land surface temperature (LST), across two residential zones within the city. This analytical approach integrates both prescribed building regulations and the physical characteristics of the extant urban fabric and configuration. Climate data were collected from the weather station in the format of EnergyPlus weather data, and LST historical data were collected from satellite imagery datasets. The results show a correlation between building geometry and UHI-related metrics, particularly evident during nocturnal periods. Notably, a negative correlation was found between the sky view factor and temperature increments. The study concludes with a strong correlation between building geometry and UHI, underscoring the imperative of integrating the building geometry and configuration considerations within the broader context of urban environmental assessments. While similar studies have been undertaken in different regions, there is a research gap in UHI within the GCC region. This study aims to contribute valuable insights to understanding urban heat island dynamics in Gulf cities. Full article

(1) Background: The rapid expansion of information technology and globalization has significantly transformed psychosocial health, with excessive internet use and the pursuit of social acceptance via social media amplifying the impact of social factors, leading to both positive and negative psychological outcomes. This paper introduces “selective sociality,” a new psychological construct focused on mindful social engagement and digital interaction to maintain psychosocial health in the digital era, supported by a valid psychometric instrument: the Selective Sociality Scale (SSS). (2) Methods: The current research was conducted in a series of eight consecutive phases involving 1737 participants (Mean age = 24 years, SD = 7.66; women = 53.3%). The validation of the SSS involved exploratory and confirmatory factor analyses along with convergent, divergent, and predictive validity. (3) Results: The SSS demonstrated excellent reliability (α = 0.838; ICC = 0.954). The model fit indices, such as CFI (0.962), TLI (0.952), RMSEA (0.059), and SRMR (0.043), showed strong validity. The scale exhibited strong convergent validity with the Efficient Social Intelligence Scale. Selective sociality predicted positive personality traits and mental health but inversely predicted depression, anxiety, and stress. (4) Conclusions: The SSS offers valuable insights for both clinical practice and research. Full article

This study investigates the combined effects of mesoscale eddies and rough sea surfaces on acoustic propagation in the eastern Arabian Sea and Gulf of Aden during summer monsoon conditions. Utilizing three-dimensional sound speed fields derived from CMEMS data, sea surface spectra from the SWAN wave model validated by Jason-3 altimetry, and the BELLHOP ray-tracing model, we quantify their synergistic impacts on underwater sound. A Monte Carlo-based dynamic sea surface roughness model is integrated with BELLHOP to analyze multiphysics interactions. The results reveal that sea surface roughness significantly influences surface duct propagation, increasing transmission loss by approximately 20 dB compared to a smooth sea surface, while mesoscale eddies deepen the surface duct and widen convergence zones by up to 5 km. In deeper waters, eddies shift convergence zones and reduce peak sound intensity in the deep sound channel. These findings enhance sonar performance and underwater communication in dynamic, monsoon-influenced marine environments. Full article

Tissue preservation plays an essential role in biomedical research and histopathological applications. Traditional methods, despite their efficiency, are associated with compromised long-term tissue integrity and probable ecotoxicities. This study explores the application of silver nanoparticles (AgNPs), known for their antimicrobial properties, as a potential tissue preservative. In this work, AgNPs were synthesized via a chemical reduction method. Heart, liver, and kidney tissues were obtained from BALB/c mice and preserved using 10% neutral buffered formalin (NBF) and AgNPs solution for 72 h. Preservation efficiency was assessed by quantifying and measuring DNA and RNA integrity, evaluating protein stability, and conducting histopathological examinations. This study aimed to compare the performance of AgNPs against 10% NBF across these parameters to determine their suitability as an alternative fixative. Our results showed that AgNPs solution maintained consistent DNA, RNA, and protein concentrations/quality across all tissues over 72 h, whereas formalin treatment led to degradation over time. Conversely, 10% NBF demonstrated better preservation of tissue morphology. These results highlighted the differential strengths of each fixative, with AgNPs excelling in molecular preservation and NBF in structural integrity. Overall, AgNPs exhibited superior qualitative and quantitative preservation of nucleic acids and intracellular proteins, indicating their potential as an alternative to formalin for molecular testing. Despite their demonstrated efficacy in biomolecular preservation, further studies are needed to optimize tissue morphology preservation. Full article

Background/Objectives: CD47 is a cell surface glycoprotein moderately expressed in healthy cells and upregulated in cancer and viral infected cells. CD47’s interaction with signal regulatory protein alpha (SIRPα) inhibits phagocytic cells and its interaction with thrombospondin-1 inhibits T cell response. Experimental evidence has revealed that the blockade of CD47 resulted in the increased activation and function of both innate and adaptive immune cells, therefore exerting antitumoral and antiviral effects. Recent studies have shown that the combination of vaccines and immune checkpoint inhibitors could be a promising approach to increasing vaccine immunogenicity. Here, we investigated the vaccinal effect of anti-CD47 antibodies and discussed the possibilities of combining anti-CD47 treatments with vaccines. Methods: Using vesicular stomatitis virus (VSV), a widely used replication-competent vaccine vector, we evaluated the impact of the immunotherapeutic blockade of CD47 on cellular, humoral, and protective immunity. We infected C57BL/6 mice with VSV, treated them with anti-CD47 antibodies or an isotype, and evaluated the total immunoglobulin (Ig), IgG neutralizing antibodies, B cell activation, CD8+ T cell effector function, and survival of the mice. Results: We found that the treatments of anti-CD47 antibodies led to significantly increased Ig and IgG neutralizing antibody levels compared to the isotype treatment. Flow cytometric analysis of B cells revealed no difference in the number of circulating B cells; however, we observed an increased surface expression of CD80 and CD86 in B cells among anti-CD47-treated mice. Further analysis of the impact of CD47 blockade on T immunity revealed a significantly higher percentage of IFN-γ⁺ CD4 and IFN-γ⁺ CD8 T cells in anti-CD47-treated mice. Upon infecting mice with a lethal VSV dose, we observed a significantly higher survival rate among the anti-CD47-treated mice compared to control mice. Conclusions: Our results indicate that anti-CD47 treatment induces a stronger cellular and humoral immune response, leading to better protection. As such, immunotherapy by CD47 blockade in combination with vaccines could be a promising approach to improve vaccine efficacy. Full article

Background/Objectives: Beetroot juice (BJ), a natural source of dietary nitrate, has gained increasing attention for its potential to improve exercise performance and cardiovascular function. While its benefits are well documented in endurance contexts, less is known about its short-term effects on resistance training performance and recovery. Thus, this study investigated the effects of short-term BJ supplementation on strength performance, cardiovascular responses, muscle oxygenation, and post-exercise recovery in resistance-trained males. Methods: Twelve healthy men (age: 21.3 ± 1.9 years; body mass index: 21.42 ± 2.36 kg/m²) completed two supplementation protocols involving BJ, providing 450 mg of nitrate per day, and a nitrate-free placebo (PLA). Each protocol consisted of two laboratory visits, one to assess the acute ergogenic effects and another to evaluate recovery after 72 h, resulting in a total of four sessions over a two-week period. During the three consecutive days of supplementation, participants ingested a single 900 mL dose (15 g BJ powder/PLA) 2 h before the first session, followed by three daily 300 mL doses (5 g BJ each/PLA) over the next two days, and a final dose (15 g BJ powder/PLA) taken 2 h before the second session (72 h post-first session). Each testing session involved incremental back squat (BS) and bench press (BP) exercises at 60%, 70%, and 80% of the one-repetition maximum (1RM) performed to failure, with three-minute rest intervals between sets. Repetition to failure, movement velocity, peak power, peak heart rate (HR), and muscle oxygenation (SmO₂) were recorded during BP and BS exercises. Heart rate variability (HRV) and blood lactate were assessed before and after each training session. Lower-limb strength (CMJ and SJ) and delayed-onset muscle soreness (DOMS) were assessed daily during the 3-day supplementation period. Results: BJ significantly increased repetitions completed at 80% 1RM during BP and BS (p < 0.05) compared to the PLA. Peak movement velocity improved across all intensities using BJ with higher values compared to the PLA at 60–80% 1RM (p < 0.05). SmO₂ was higher in BJ at 70–80% 1RM) and further improved after 72 h of BJ supplementation (p < 0.05). Cardiovascular strain was reduced in BJ, evidenced by lower peak HRs and smaller post-exercise declines in HRV indices (p < 0.05). Post-exercise recovery favored BJ, with faster recovery in jump performance at 24 h and reduced upper-limb DOMS at 24–48 h (p < 0.05). Conclusions: Short-term BJ supplementation enhances high-intensity resistance performance, improves muscle oxygenation, attenuates cardiovascular strain, and accelerates neuromuscular recovery. These benefits highlight its potential as a practical strategy for athletes seeking to optimize training performance and recovery during periods of intense resistance training. Full article