Search Results (24)

This paper presents the development of two solutions for sandwich panels composed of a thin-layer alkali-activated composite (AAc) layer and a thicker insulation layer, formed by extruded polystyrene foam or expanded cork agglomerate (panels named AP_XPS or AP_ICB, respectively). The AAc combined ceramic waste from clay bricks and roof tiles (75%) with ladle furnace slag (25%), activated with sodium silicate. The AAc layer was further reinforced with polyacrylonitrile (PAN) fibers (1% content). The mechanical behavior was assessed by measuring the uniaxial compressive strength of cubic AAc specimens, shear bond strength, pull-off strength between the AAc layer and the insulation material, and the flexural behavior of the sandwich panels. The thermal performance was characterized by heat flux, inner surface temperatures, the thermal transmission coefficient, thermal resistance, and thermal conductivity. Mechanical test results indicated clear differences between the two proposed solutions. Although AP_XPS panels exhibited higher tensile bond strength values, the AP_ICB panels demonstrated superior interlayer bond performance. Similar findings were observed for the shear bond strength, where the irregular surface of the ICB positively influenced the adhesion to the AAc layer. In terms of flexural behavior, after the initial peak load, the AP_XPS exhibited a deflection-hardening response, achieving greater load-bearing capacity and energy absorption capacity compared to the AP_ICB. Finally, thermal resistance values of 1.02 m² °C/W and 1.14 m² °C/W for AP_ICB and AP_XPS were estimated, respectively, showing promising results in comparison to currently available building materials. Full article

This study addresses the unique challenge of the partition walls in subway stations, featuring high height, fire prevention, and located outside the main frames, by introducing a confined autoclaved aerated concrete (AAC) panel wall system. Different from studies on a main frame with infill walls, this study aimed to explore the seismic performance of partition walls, which were fabricated with confined high AAC panel walls and located outside the main frames. A custom-designed partition wall, measuring 6600 mm in height, 3400 mm in width, and 200 mm in thickness, underwent cyclic testing. A detailed analysis of specimen’s failure modes was conducted, focusing on seismic behavior such as hysteresis curves, envelope curves, ductility, stiffness degradation, and energy-dissipation capacity. Additionally, the study delved into shear deformation, relative slippage between AAC panels, and reinforcement strains within the specimen. Finally, the D-value method for calculating the initial stiffness of the confined high AAC panel walls and the weak sub-structural approach for determining the load-bearing capacity of confined high AAC panel walls were proposed and validated. The results indicate that the strength degradation factor of the confined high AAC panel walls ranges from 0.971 to 0.716. The drift of its upper portion accounts for 76.94–83.63% of the total drift, while the energy dissipation factor of its upper portion is 0.8–4.8% higher than that of the entire specimen. The yield and ultimate drift rotations of the entire confined high AAC panel wall and its upper portions satisfy the elastic and elastic-plastic inter-story drift rotation limits specified in the Chinese code. The calculated initial stiffness of the confining frame, obtained using the D-value method, closely aligns with experimental results, with a deviation of only 2.48%. Additionally, the load-bearing capacity calculated using the weak sub-structural approach deviates from the experimental average by just 4.30%. Full article

Background: Whole genome sequencing of clinical bacterial isolates holds promise in predicting their susceptibility to antibiotic therapy, based on a detailed understanding of the phenotypic manifestation of genotypic variation. The aac(6′) aminoglycoside acetyltransferase gene family is the most abundant aminoglycoside resistance determinant encountered in clinical practice. A variety of AAC(6′) isozymes have been described, suggesting a phenotypic distinction between subtype I, conferring resistance to amikacin (AMK), and subtype II, conferring resistance to gentamicin (GEN) instead. However, the epidemiology and thus clinical relevance of the various and diverse isozymes and their phenotypic distinction demand systematic and contemporary re-assessment to reliably predict bacterial susceptibility to aminoglycoside antibiotics. Methods: We analyzed the resistance gene annotations of 657,603 clinical bacterial isolates to assess the prevalence and diversity of aac(6′) genes. Seventeen unique aac(6′) amino acid sequences were cloned and expressed under defined promoter control in otherwise isogenic E. coli cells for phenotypic analysis with twenty distinct aminoglycoside antibiotics. A panel of clinical isolates was analyzed for the genotype–phenotype correlation of aac(6′). Results: An aac(6′) resistance gene annotation was found in 139,236 (21.2%) of the clinical isolates analyzed. AMK resistance-conferring aac(6′)-I genes dominated in Enterobacterales (28.5%). In Pseudomonas aeruginosa and Acinetobacter baumannii, a gene conferring the aac(6′)-II phenotype but annotated as aac(6′)-Ib₄ was the most prevalent. None of the aac(6′) genes were annotated as subtype III, but gene aac(6′)-Ii identified in Gram-positive isolates displayed a subtype III phenotype. Genes that were annotated as aac(6′)-Ib₁₁ in Enterobacterales conferred resistance to both AMK and GEN, which we propose constitutes a novel subtype IV when applying established nomenclature. A phenotypic assessment facilitated structural re-assessment of the substrate promiscuity of AAC(6′) enzymes. Conclusions: Our study provides the most comprehensive analysis of clinically relevant aac(6′) gene sequence variations to date, providing new insights into a differentiated substrate promiscuity across the genotypic spectrum of this gene family, thus translating into a critical contribution towards the development of amino acid sequence-based in silico antimicrobial susceptibility testing (AST). Full article

The clinical significance of numerous cardiovascular gene variants remains to be determined. CRISPR/Cas9 allows for the introduction and/or correction of a certain variant in induced pluripotent stem cells (iPSCs). The resulting isogenic iPSC lines can be differentiated into cardiomyocytes and used as a platform to assess the pathogenicity of the variant. In this study, isogenic iPSC lines were generated for a variant of unknown significance found previously in a patient with hypertrophic cardiomyopathy (HCM), p.N515del (c.1543_1545delAAC) in MYBPC3. The deletion was corrected with CRISPR/Cas9 in the patient-specific iPSCs. The iPSC lines with the corrected deletion in MYBPC3 maintained pluripotency and a normal karyotype and showed no off-target CRISPR/Cas9 activity. The isogenic iPSC lines, together with isogenic iPSC lines generated earlier via introducing the p.N515del (c.1543_1545delAAC) variant in MYBPC3 of iPSCs of a healthy donor, were differentiated into cardiomyocytes. The cardiomyocytes derived from both panels of the isogenic iPSCs showed an increased size in the presence of the deletion in MYBPC3, which is one of the HCM traits at the cellular level. This finding indicates the effectiveness of these iPSC lines for studying the impact of the variant on HCM development. Full article

Autoclaved aerated concrete (AAC) has gained widespread acceptance in construction as a lightweight solution for exterior and interior walls. However, traditional steel-reinforced autoclaved aerated concrete (SR-AAC) has limitations, including concerns over its ductility and difficulty in cutting during installation. The steel reinforcement also has high embodied carbon that does not align with the actions in the construction section to reach carbon neutrality shortly. This study investigated the material properties and mechanical performances of factory-produced fiber-reinforced autoclaved aerated concrete (FR-AAC) panels, aiming to examine their potential as an alternative solution. Full-scale FR-AAC panels with thicknesses of 100 mm, 150 mm, and 200 mm were manufactured and tested. Some panels were down-sampled to determine the dry density, water absorption, compressive strength, and flexural strength of the material, while the mechanical performances were evaluated through static and impact loading tests. The results showed that the average dry density and absorption of the FR-AAC material are 533 kg/m³ and 63%, respectively, with compressive strengths up to 3.79 MPa and flexural strengths reaching 0.97 MPa. All six panels tested under static uniformly distributed loading exceeded the self-weight limit by a factor of 1.5, satisfying standard requirements for load-bearing capacity. However, the brittle failure modes observed in some tests raise potential health and safety concerns. In contrast, the impact tests revealed that the panels have acceptable performances with the inclusion of fibers. Full article

Prefabricated panel-assembled wall systems, comprising a confining frame and infill lightweight panels of autoclaved aerated concrete (AAC), are widely employed in framed structures. Different from studies on a main frame with infill walls, this study aimed to explore the seismic performance of partition walls, which were fabricated with AAC panel-assembled walls and located outside of the main frames. Two full-scale specimens, one with a door opening and the other without, were constructed and cyclic loading tests were executed to examine the failure modes, hysteresis characteristics, envelope curves, ductility, strength and stiffness degradation, as well as energy dissipation capacity of the AAC panel-assembled walls. Additionally, a restoring-force model for the panel-assembled walls was developed and a method for predicting the lateral load-bearing capacity of the AAC panel-assembled walls was proposed. The findings indicated that the panels enhanced the system’s lateral resistance, energy dissipation capacity, and deformation capability. The door frame increased the initial stiffness, peak lateral load and energy dissipation capacity of the AAC panel-assembled wall compared to the wall without a door frame. Compared to the specimen without a door frame, the peak lateral load of the specimen with a door frame increased by 19.7–30.1%. The deformation capacity of the panel-assembled walls aligned with the requirements for concrete framed structures. Full article

Antimicrobial resistance (AMR) is an emerging public health threat and is one of the One Health priorities for humans, animals, and environmental health. Red foxes (Vulpes vulpes) are a widespread predator species with great ecological significance, and they may serve as a sentinel of antimicrobial resistance in the general environment. The present study was carried out to detect antimicrobial resistance, antimicrobial resistance genes, and genetic diversity in faecal isolates of red foxes (Vulpes vulpes). In total, 34 Enterococcus isolates, including E. faecium (n = 17), E. faecalis (n = 12), E. durans (n = 3), and E. hirae (n = 2), were isolated. Antimicrobial resistance to 12 antimicrobial agents was detected with EUVENC panels using the minimum inhibitory concentration (MIC). The presence of antimicrobial resistance genes (ARGs) was determined using whole-genome sequencing (WGS). Resistance to tetracycline (6/34), erythromycin (3/34), ciprofloxacin (2/34), tigecycline (2/34), and daptomycin (2/34) was identified in 44% (15/34) of Enterococcus isolates, while all the isolates were found to be susceptible to ampicillin, chloramphenicol, gentamicin, linezolid, teicoplanin, and vancomycin. No multi-resistant Enterococcus spp. were detected. A total of 12 ARGs were identified in Enterococcus spp., with the presence of at least 1 ARG in every isolate. The identified ARGs encoded resistance to aminoglycosides (aac(6′)-I, ant(6)-Ia, aac(6′)-Iih and spw), tetracyclines (tet(M), tet(L) and tet(S)), and macrolide–lincosamide–streptogramin AB (lnu(B,G), lsa(A,E), and msr(C)), and their presence was associated with phenotypical resistance. Core genome multilocus sequence typing (cgMLST) revealed the high diversity of E. faecalis and E. faecium isolates, even within the same geographical area. The distribution of resistant Enterococcus spp. in wild foxes in Latvia highlights the importance of a One Health approach in tackling AMR. Full article

Pseudomonas aeruginosa is among the most ubiquitous bacteria in the natural world, exhibiting metabolic and physiological versatility, which makes it highly adaptable. Imipenem + cilastatin and tetracycline are antibiotic combinations commonly used to treat infections caused by P. aeruginosa, including serious infections such as sepsis. In the context of bacterial infections, biofilm, formed by bacterial cells surrounded by extracellular substances forming a matrix, plays a pivotal role in the resistance of P. aeruginosa to antibiotics. This study aimed to characterize a representative panel of P. aeruginosa isolates from septicemias, assessing their susceptibility to various antibiotics, specifically, imipenem + cilastatin and tetracycline, and the impact of these treatments on biofilm formation. Results from antibiotic susceptibility tests revealed sensitivity in most isolates to six antibiotics, with four showing near or equal to 100% sensitivity. However, resistance was observed in some antibiotics, albeit at minimal levels. Notably, tetracycline showed a 100% resistance phenotype, while imipenem + cilastatin predominantly displayed an intermediate phenotype (85.72%), with some resistance (38.1%). Microdilution susceptibility testing identified effective combinations against different isolates. Regarding biofilm formation, P. aeruginosa demonstrated the ability to produce biofilms. The staining of microtiter plates confirmed that specific concentrations of imipenem + cilastatin and tetracycline could inhibit biofilm production. A significant proportion of isolates exhibited resistance to aminoglycoside antibiotics because of the presence of modifying genes (aac(3)-II and aac(3)-III), reducing their effectiveness. This study also explored various resistance genes, unveiling diverse resistance mechanisms among P. aeruginosa isolates. Several virulence genes were detected, including the las quorum-sensing system genes (lasI and lasR) in a significant proportion of isolates, contributing to virulence factor activation. However, genes related to the type IV pili (T4P) system (pilB and pilA) were found in limited isolates. In conclusion, this comprehensive study sheds light on the intricate dynamics of P. aeruginosa, a remarkably adaptable bacterium with a widespread presence in the natural world. Our findings provide valuable insights into the ongoing battle against P. aeruginosa infections, highlighting the need for tailored antibiotic therapies and innovative approaches to combat biofilm-related resistance. Full article

This study aimed to investigate the occurrence and characteristics of Salmonella isolates in salad vegetables in the United Arab Emirates (UAE). Out of 400 samples tested from retail, only 1.25% (95% confidence interval, 0.41–2.89) were found to be positive for Salmonella, all of which were from conventional local produce, presented at ambient temperature, and featured as loose items. The five Salmonella-positive samples were arugula (n = 3), dill (n = 1), and spinach (n = 1). The Salmonella isolates from the five samples were found to be pan-susceptible to a panel of 12 antimicrobials tested using a disc diffusion assay. Based on whole-genome sequencing (WGS) analysis, only two antimicrobial resistance genes were detected—one conferring resistance to aminoglycosides (aac(6′)-Iaa) and the other to fosfomycin (fosA7). WGS enabled the analysis of virulence determinants of the recovered Salmonella isolates from salad vegetables, revealing a range from 152 to 165 genes, collectively grouped under five categories, including secretion system, fimbrial adherence determinants, macrophage-inducible genes, magnesium uptake, and non-fimbrial adherence determinants. All isolates were found to possess genes associated with the type III secretion system (TTSS), encoded by Salmonella pathogenicity island-1 (SPI-1), but various genes associated with the second type III secretion system (TTSS-2), encoded by SPI-2, were absent in all isolates. Combining the mean prevalence of Salmonella with information regarding consumption in the UAE, an exposure of 0.0131 salmonellae consumed per person per day through transmission via salad vegetables was calculated. This exposure was used as an input in a beta-Poisson dose–response model, which estimated that there would be 10,584 cases of the Salmonella infection annually for the entire UAE population. In conclusion, salad vegetables sold in the UAE are generally safe for consumption regarding Salmonella occurrence, but occasional contamination is possible. The results of this study may be used for the future development of risk-based food safety surveillance systems in the UAE and to elaborate on the importance for producers, retailers, and consumers to follow good hygiene practices, particularly for raw food items such as leafy salad greens. Full article

We developed two multiplex polymerase chain reactions (PCRs) for the detection of extended-spectrum β-lactamases (ESBLs), plasmid-mediated AmpC β-lactamases, aac(6′)-Ib gene, and integrase genes (intI1, intI2, and intI3) in class 1, 2, and 3 integrons in Gram-negative bacteria. We evaluated the PCRs using 109 Gram-negative isolates from non-organic (ANO) and organic (AO) vegetables and fruits. Screening of ANO substances identified five SHV, one TEM-1, one CTX-M, 20 AmpC-CS, and two intI1 positives. DNA sequencing revealed CTX-M in Pantoea spp. was bla_RANH-2, a plasmid-mediated CTX-M related ESBL gene only found in Rahnella spp. Of the 20 AmpC-CS positives, 10 were CMY/MIR/ACT/EC (3 new variants), eight were ACT, one was AZECL, and one was new Pseudomonas-related AmpC family. Screening of AO substances identified 11 SHV, two TEM-1, three CTX-M (one OXY-2, two CTX-M-14/-15), two OXA-9, 13 AmpC-CS and one intI1 positives. The 13 AmpC-CS positives were five CMY/MIR/ACT/EC, three ACT, one MOX-12 variant, and four ADC (one ADC-25 and three new variants). We developed a rapid, easy-to-perform, low-cost, and reliable multiplex PCR system for screening clinically relevant β-lactamases and integrons in Gram-negative bacteria. We showed the prevalence of ESBLs and AmpC β-lactamases among our panel of ampicillin-resistant Gram-negative strains and detection of NDM and OXA carbapenemases. Full article

This study proposes a new energy dissipation connector (NEDC) to connect an external autoclaved aerated concrete (AAC) wall panel with an assembled steel frame. To investigate the seismic performance and working mechanism of the NEDC under seismic action, horizontal low-cyclic loading tests were conducted on two sets of steel frames with different connectors using an MTS actuator. Similarly, the seismic performance and working mechanism of the AAC wall panels were elucidated using horizontal low-cyclic loading tests. Test results revealed that the NEDC increased ductility by 10.69–21.12% and energy consumption by 101.14% when compared to those obtained using hook bolt connectors. Overall, the NEDC provides good seismic performance, large deformability, and high energy consumption capacity, thereby rendering it ideal for assembled steel buildings. Furthermore, the NEDC can reduce wall panel damage during earthquake action and enhance the seismic performance of composite frames. Full article

Augmentative and alternative communication (AAC) devices enable children and adolescents (CAD) with communication disorders to communicate competently and develop friendships through communicative competence (CC). Existing assessment tools are unable to indicate whether CAD aged 0 to 18 years would competently use the subsidized AAC devices provided by the Ministry of Health and Welfare in Taiwan. This study, thus, aimed to develop an assessment tool by using the International Classification of Functioning, Disability and Health (ICF) to measure CC in dyadic communication among CAD using AAC devices. Five speech-language pathologists (SLPs), five special education teachers, and four AAC experts (14 in total) selected codes relevant to the four domains of CC via the Delphi method. Next, they categorized the selected codes into one of the four domains of CC through a face-to-face expert panel. A total of 112 codes were listed in the tool and fully classified into the four domains of CC. Among these, seven codes were concurrently placed under two domains of CC. Consequently, this study developed an assessment tool by employing the ICF for children and youth core set using universal qualifiers to measure the relative levels of CC in dyadic communication among CAD who use AAC devices in their daily life. Full article

Development of novel anticancer therapeutic candidates is one of the key challenges in medicinal chemistry. Podophyllotoxin and its derivatives, as a potent cytotoxic agent, have been at the center of extensive chemical amendment and pharmacological investigation. Herein, a new series of podophyllotoxin-N-sulfonyl amidine hybrids (4a–4v, 5a–5f) were synthesized by a CuAAC/ring-opening procedure. All the synthesized podophyllotoxins derivatives were evaluated for in vitro cytotoxic activity against a panel of human lung (A-549) cancer cell lines. Different substituents’, or functional groups’ antiproliferative activities were discussed. The –CF₃ group performed best (IC₅₀: 1.65 μM) and exhibited more potent activity than etoposide. Furthermore, molecular docking and dynamics studies were also conducted for active compounds and the results were in good agreement with the observed IC₅₀ values. Full article

Background: Air pollution is one source of harm to the health of residents, and the impact of air pollution on health expenditure has become a hot topic worldwide. However, few studies aim at the spatial spillover effects of air pollution on the health expenditure of rural residents (HE-RR), including the impact on the health expenditure in neighboring areas. Objective: Based on the existing research, this paper further introduces the spatial dimension and uses the Spatial Durbin model to discuss the impact of environmental pollution on the health expenditure of rural residents (HE-RR). Methods: Based on provincial panel data during 2002–2015 in China, the Spatial Durbin model was used to investigate the spatial spillover effect of the average annual concentration of PM2.5 (AAC-PM2.5) on the health expenditure of rural residents (HE-RR). Results: There was a significant positive correlation between AAC-PM2.5 and health expenditure of rural residents (HE-RR) in neighboring areas at a significant level of 5% (COEF: 2.546, Z: 2.340), that is, AAC-PM2.5 has a spatial spillover effect on PC-HE-RR in neighboring areas, and the spatial spillover effect is greater than the direct effect. The migration and diffusion of PM2.5 pollution will affect the air quality of neighboring areas, leading to the health risk not only from the local PM2.5 pollution but also the nearby PM2.5 pollution. Conclusion: The results show a significant positive relationship between air pollution and HE-RR in neighboring areas, and the spatial spillover effect is greater than the direct effect. Full article

Mechanical response of textile-reinforced aerated concrete sandwich panels was investigated using instrumented three-point bending tests under quasi-static and low-velocity impact loads. Two types of core material were compared in the sandwich composite consisting of plain autoclaved aerated concrete (AAC) and fiber-reinforced aerated concrete (FRAC), and the stress skins were alkali-resistant glass (ARG) and textile reinforced concrete (TRC). The textile-reinforced layer promoted distributed cracking mechanisms and resulted in significant improvement in the flexural strength and ductility. Digital Image Correlation (DIC) was used to study the distributed cracking mechanism and obtain impact force-crack width response at different drop heights. A constitutive material model was also developed based on a multi-linear tension/compression strain hardening model for the stress-skin and an elastic, perfectly plastic compression model for the core. A detailed parametric study was used to address the effect of model parameters on the flexural response. The model was further applied to simulate the experimental flexural data from the static and impact tests on the plain aerated concrete and sandwich composite beams. Full article