Search Results (148)

Microbially Induced Carbonate Precipitation (MICP) is a biochemical process that promotes the precipitation of calcium carbonate, mainly in the mineral form of calcite, using urease-producing bacteria. This method has numerous applications, particularly in the field of geotechnical engineering when it is adopted for soil improvement or for the consolidation of porous or cracked construction materials such as stone and concrete. One microfluidic platform made of polymethylmethacrylate (PMMA) was designed with multiple channels, and the minerals precipitated were visualized using an optical microscope. The precipitated mineral observed in all channels analyzed formed spherical mineral structures with a core and multiple external rings. The same spherical mineral structures were observed in the biocement layer precipitated on plates of the same material as that of the microfluidic platform and on limestone, following the same treatment protocol. SEM images of pieces of these layers, complemented with EDS and mineral analysis by XRD, have confirmed the existence of multiple layers of minerals with spherical structures, mainly vaterite, precipitated around a nucleation point. Overlapping minerals in both the confined microfluidic channels and the unconstrained plates indicate that overlap results from repeated injections rather than physical confinement. From the tests with the microfluidic devices, these studies revealed that crystallization depends on different factors, namely the size of the channels and the number of Sporosarcina pasteurii cells. The number of injections appeared to affect the number of rings precipitated around the inner core. Substrate effects on spatial distribution or adhesion may still exist but were not detectable in this study and require further investigation. The observation of similar mineralogical structures in both the microfluidic devices and the plates, particularly the limestone, demonstrates that microfluidic systems are effective tools for small-scale visualization of geological processes. Full article

Ferruginous bromalites (coprolites and cololites) occur in enormous quantities in the Upper Cretaceous Whitemud Formation of Saskatchewan, Canada, and in Miocene deposits in Madagascar and southwest Washington, USA. The origins of these specimens have been the subject of diverse and often conflicting interpretations. This paper includes some discussion of other localities, but the main focus is on specimens from Wilkes Formation at Salmon Creek, Lewis County, Washington State, USA. This locality is notable because the geologic setting and paleoenvironment are well-established, and the purported bromalites can be observed in situ, providing stratigraphic and taphonomic information that is not available for the Canada and Madagascar locations. Past research at Salmon Creek has a curious history. Supporters of the coprolite interpretation have relied on Salmon Creek specimens collected by others. In contrast, field-based investigators have concluded that the extruded objects are probably pseudofossils. Was the origin of these objects biotic excretion or abiotic extrusion? Past evidence is not sufficient for resolving this issue. New information strengthens the abiotic interpretation, but these ferruginous specimens remain as a geologic enigma. Full article

This study examines key bioprocess parameters influencing the reduction in hydraulic conductivity in porous media via Microbially-Induced Calcite Precipitation (MICP), highlighting its relevance to environmental engineering applications such as bio-barriers and landfill liners. Sporosarcina pasteurii was utilized as the ureolytic bacterium to induce calcium carbonate precipitation under controlled laboratory conditions. Experimental variables included bacterial cell density (OD₆₀₀), diameter of glass beads, concentrations of precipitation solution, bentonite, and yeast extract. A total of 42 experimental runs were conducted based on a custom design in Design-Expert software. Hydraulic conductivity was selected as the response variable to evaluate treatment performance. Response surface methodology (RSM) was applied to develop a second-order polynomial model, with statistical analyses indicating a strong model fit (R² = 0.948, adjusted R² = 0.929, predicted R² = 0.868). ANOVA confirmed the significance of the main effects and interactions, particularly those involving glass bead diameter and OD₆₀₀. Among the tested factors, the precipitation solution exhibited the strongest individual effect, while bentonite and yeast extract demonstrated supportive roles. Optimization revealed that a balanced combination of microbial density and chemical inputs minimized hydraulic conductivity to 0.0399 cm/s (≈95% reduction), with an overall desirability score of 1.000. Laboratory-scale experiments demonstrated field-scale applicability, underscoring the potential of biotechnological soil treatment and empirical modeling for developing sustainable low-permeability barriers. Full article

Serpulids are among the few annelid groups capable of building skeletal structures by secreting calcium carbonate. Compared with other biomineralizing organisms, their control over tube construction is relatively limited, making them vulnerable to environmental changes. To distinguish between intrinsic biological regulation and extrinsic environmental influence in tube formation, we examine the calcareous tube of Hydroides elegans, focusing on the tube ultrastructure, mineral composition, elemental distribution, organic-inorganic constituents, and biomineralization mechanism. The results show that the tube consists of three superimposed layers: an innermost organic sheet, an intermediate lamello-fibrillar calcite layer, and an outermost spherulitic prismatic calcite layer. The outer spherulitic prismatic layer frequently exhibits bioerosion, trapped sedimentary particles, and fan-shaped aragonite aggregates, indicating pronounced environmental influence. In contrast, the middle lamello-fibrillar calcite fabric is highly organized and closely integrated with the innermost organic sheet, indicating strictly biological controls. This study highlights the combined effect of biological controls and environmental influences in serpulid tube calcification, contributing to our understanding of their adaptive evolution in changing oceans. Full article

Identifying stratigraphic continuity across outcrops can sometimes be difficult, especially if they are dominated by discontinuous strata. Therefore, stratigraphers continue to seek new proxies for testing stratigraphic continuity, including fossiliferous horizons. We present a case study examining the potential of fossil bone trace element signatures as a novel proxy for lateral continuity. Specifically, we performed trace element analyses of Edmontosaurus bones from the Neufeld Quarry at Hanson Ranch (HR) in Wyoming, a stratigraphically verified lateral equivalent of the famous HR Bonebed exposed nearby in five “Main Quarries”, to evaluate if these chemical data would independently lead a researcher to the same conclusion of lateral equivalency. Bones from the “Main Quarries” and Neufeld were found to exhibit similar patterns of trace element alteration, including comparable magnitudes of enrichment, spatial patterns of rare earth element uptake, and proportions of specimens exhibiting various styles of diagenetic alteration. Many bones from both sites also exhibit redox signatures indicative of trace element uptake under reducing conditions. These numerous similarities in geochemical alteration patterns independently indicate that the fossil horizon at Neufeld is a lateral continuation of the nearby HR Bonebed. Our findings thus demonstrate the power of trace elements toward identifying laterally equivalent fossil assemblages. Full article

For paleoenvironmental reconstruction, paleontologists prefer large, well-preserved fossils. Yet, such specimens are rare, and countless small fragments, though abundant, often go unused. These fragments lack visible internal structure, thus requiring etching, a procedure not permitted on large, intact specimens. Our research introduces a three-step method to identify the nature of these small fragments. With their structures revealed, we can then analyze the chemical composition of identified tissues. The method was tested using samples of vertebrate fossils collected in Malawi. Even with a limited number of samples, multivariate analyses (Principal Component Analyses—PCA) of these chemical data effectively differentiate fossil and recent samples, as well as bone, dentin, and enamel. This approach successfully reveals the behavior of the mineralized tissues of fossil samples. Ultimately, by leveraging microstructural and chemical data, we can study previously unidentified fragments or rare fossils. This allows for the estimation of preservation state and helps to avoid biases in paleoenvironmental reconstructions. Full article

Calcium–magnesium (Ca–Mg) carbonates are among the most widely distributed carbonates in the Earth’s surface environment, and their formation mechanisms are of great significance for revealing geological environmental changes and carbon sequestration processes. In this study, the gas diffusion method was employed with L-glutamic acid, L-glycine, and L-lysine as nucleation templates for carbonate minerals to systematically investigate their regulatory effects on the mineralization of Ca–Mg carbonates. The results demonstrated that L-glycine, with the shortest length, was more conducive to forming aragonite, whereas acidic L-glutamic acid, which contains more carboxyl groups, was more beneficial for the structural stability of aragonite. The morphology of the Ca-Mg carbonate minerals became more diverse and promoted the formation of spherical and massive mineral aggregates under the action of amino acids. Moreover, the amino acids significantly increased the MgCO₃ content in Mg calcite (L-glutamic acid: 10.86% > L-glycine: 7.91% > L-lysine: 6.63%). The acidic L-glutamic acid likely promotes the dehydration and incorporation of Mg²⁺ into the Mg calcite lattice through the preferential adsorption of Mg²⁺ via its side-chain carboxyl groups. This study shows how amino acid functional groups influence Ca–Mg carbonate mineralization and provides insights into biogenic Mg-rich mineral origins and advanced mineral material synthesis. Full article

This study investigates the dynamic operation of a pilot-scale precipitation reactor designed to produce oxygenated phosphocalcium apatites with controlled composition and low crystallinity, closely mimicking the mineral phase of bone. Our approach is based on integrating kinetic monitoring and dynamic reactor control to direct the formation of apatites with tailored structural and chemical properties. Three synthesis routes were explored using CaCO₃, Ca(NO₃)₂, and CaCl₂ as calcium precursors, under optimized Ca/P molar ratios. The evolution of ionic concentrations (Ca²⁺, PO₄³⁻), peroxide and molecular oxygen incorporation, and carbonate content was monitored over a reaction time range of 2 min to 4 h. Characterization by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and chemical analysis revealed a time-dependent transformation of amorphous phases into poorly crystalline apatites with specific textures. After 60 min, the Ca/P atomic ratio stabilized at approximately 1.575, and the resulting apatites exhibited structural features comparable to those of human bone. This study highlights the influence of reactor operation time on precipitation kinetics and the properties of bioactive apatites in a scalable system. The results offer promising prospects for the large-scale production of bone-mimetic materials. However, the lack of biological validation remains a limitation. Future studies will assess the cytocompatibility and bioactivity of these materials to confirm their potential for biomedical applications. Full article

This study systematically investigates for the first time the effects of dual-site co-cultivation on spectral characteristics and trace element enrichment in marine-cultured Akoya pearls from Beihai, China. Akoya pearls were cultured over a one-year period, with the final 40-day stage designated as the terminal phase. During this period, two experimental groups of pearl oysters were established: Group Y remained in Beihai for continued local cultivation and harvest, while Group B was transferred to Weihai, Shandong Province, for terminal-stage farming under different thermal conditions. A series of comparative analyses were performed using Fourier-transform infrared (FTIR) spectroscopy, ultraviolet-visible (UV-Vis) spectroscopy, Raman spectroscopy, X-ray fluorescence (XRF), and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The FTIR results revealed distinct differences between the two groups in the distribution of amide and polysaccharide functional groups, particularly around 1643 cm⁻¹ and 1100 cm⁻¹. The UV-Vis spectra of Group B displayed characteristic absorption bands at 430 nm and 460 nm, associated with the organic matrix of the nacre. Raman spectroscopy further indicated a higher abundance of organic-related vibrational features in Group B. Additionally, both XRF and LA-ICP-MS analyses consistently showed significant differences in the concentrations and distributions of trace elements, particularly copper (Cu), cobalt (Co), and zinc (Zn). The findings demonstrate that the dual-site co-cultivation mode significantly impacts both the organic composition and trace element enrichment patterns in seawater Akoya pearls. This research provides valuable references for optimizing environmental parameters in pearl cultivation processes. Full article

Tubular fossils are a unique metazoan group emerging in the late Ediacaran Period and demonstrating early skeletogenesis and an increase in the diversity of early biocommunities. Among the known records, Sinotubulites is widely distributed and distinct in morphology and ultrastructure, holding important evolutionary and stratigraphic significance comparable to the well-known Cloudina. However, its biological affinity remains unclear until now. Among various reasons, taphonomic bias is one of the major factors responsible for this, as it not only altered the primary morphology but also modified the ultrastructure and composition of the fossil. Thus, a further study on its taphonomic process would help to decode the biological affinity of Sinotubulites. For this purpose, we conducted a taphonomic study on Sinotubulites from the top of the Shibantan Member of the Dengying Formation at the Zhongling section in the Yangtze Gorges area (Hubei Province, China). We applied electron backscatter diffraction (EBSD) and cathodoluminescence (CL) to reveal its mineralogical features. EBSD and CL analyses demonstrate that both the fossils and matrix are composed of unoriented calcite, and the matrix shows slight dolomitization with sporadic dolomite grains. The calcite crystals within the Sinotubulites tubes are significantly larger than those in the matrix, indicating that the tubular structure provided sufficient space for crystal growth. The absence of lamellar structures in the tubular walls further suggests that the original biogenic material may have been dissolved during diagenetic calcification. The absence of dolomitization in the fossils indicates that this process may have been inhibited by either their large calcite crystals or the enclosed space confined by the outer shell. The identical non-luminescent features of the matrix and fossils suggest that their calcification likely occurred during the same stage. This study demonstrates that taphonomic biases must be accounted for when analyzing the original structure and composition. Additionally, this research documents the occurrence of Sinotubulites in the Shibantan Member, representing its lowest stratigraphic horizon in the Yangtze Block. Full article

The growing scarcity of freshwater has accelerated the global deployment of desalination technologies, especially reverse osmosis (RO), as an alternative to meet increasing water demands. However, this process generates substantial quantities of brine—a hypersaline waste stream that can severely impact marine ecosystems if improperly managed. This perspective review explores the use of urease-driven Microbially Induced Carbonate Precipitation (MICP) as a biotechnological solution aligned with circular economy principles for the treatment and valorization of RO brines. Through the enzymatic activity of ureolytic microorganisms, MICP promotes the precipitation of calcium carbonate and other mineral phases, enabling the recovery of valuable elements and reducing environmental burdens. Beyond mineral capture, MICP shows promise in the stabilization of toxic metals and potential integration with microbial electrochemical systems for energy applications. This review summarizes current developments, identifies existing challenges, such as microbial performance in saline conditions and reliance on conventional urea sources, and proposes future directions focused on strain optimization, nutrient recycling, and process scalability for sustainable implementation. Full article

Concretions represent an exceptional mode of fossil preservation. This is attributed to their mineralized outer mantle, which exhibits low permeability and porosity, thereby limiting diagenetic alteration. The present research employs microscopic Raman spectroscopy to assess the thermal maturity of kerogen—a highly sensitive organic material—within concretions from northeast Taiwan. Comparative analysis of kerogen from the concretion’s core, rim, and surrounding matrix reveals differential preservation states. The organic matter in the core remains relatively unaltered, whereas the rim exhibits partial graphitization, albeit to a lesser extent than the surrounding matrix. These findings indicate a progressive diagenetic gradient, with the core influenced by the least thermal alteration, followed by the rim, and the surrounding matrix that experiences the highest degree of graphitization. Therefore, the present research underscores the role of concretionary encapsulation in mitigating diagenetic modification and enhancing organic matter preservation. Full article

Costa Rica is located along the narrow isthmus that connected South America to North America beginning in the mid-Cenozoic. The exchange of vertebrates between the two continents has received considerable study, but paleobotanical aspects are less known. The Pacific coast “ring of fire” volcanoes produced abundant hyaloclastic material that provided a source of silica for wood petrifaction, and the tropical forests contained diverse taxa. This combination resulted in the preservation of petrified wood at many sites in Costa Rica. Fossil wood ranges in age from Lower Miocene to Middle Pleistocene, but Miocene specimens are the most common. Our research involved the study of 54 specimens, with the goal of determining their mineral compositions and interpreting the fossilization processes. Data came from thin-section optical microscopy, SEM images, and X-ray diffraction. Two specimens were found to be mineralized with calcite, but most of the woods contained crystalline quartz and/or opal-CT. The preservation of anatomical detail is highly variable. Some specimens show evidence of decay or structural deformation that preceded mineralization, but other woods have well-preserved cell structures. This preliminary study demonstrates the abundance and botanical diversity of fossil wood in Costa Rica, hopefully opening a door into future studies that will consider the taxonomy and evolutionary aspects of the country’s fossil forests. Full article