Search Results (121)

In this work, we demonstrate high-efficiency 385 nm AlGaN-based near-ultraviolet micro light emitting diode (NUV-Micro LED) arrays. The epi structure is prepared using a novel AlN-inserted superlattice electrical blocking layer which enhances hole spreading in the p-type region significantly. The NUV-Micro LED arrays in this work comprise 228 chips in parallel with wavelengths at 385 nm, and each single chip size is 15 × 30 μm². Compared with conventional bulk AlGaN-based EBL structures, the NUV-Micro LED arrays that implemented the new hole spreading enhanced superlattice electrical blocking layer (HSESL-EBL) structure proposed in this work had a remarkable increase in light output power (LOP) at current density, increasing the range down from 0.02 A/cm² to as high as 97 A/cm². The array’s light output power is increased up to 1540% at the lowest current density 0.02 A/cm², and up to 58% at the highest current density 97 A/cm², measured under room temperature (RT); consequently, the WPE is increased from 13.4% to a maximum of 30.18%. This AlN-inserted HESEL-EBL design significantly enhances both the lateral expansion efficiency and the hole injection efficiency into the multi quantum well (MQW) in the arrays, improving the concentration distribution of the holes in MQW while maintaining good suppression of electron leakage. The array’s efficiency droop has also been greatly reduced. Full article

The role of rhizosphere bacteria in facilitating plant invasion is increasingly acknowledged, yet the influence of specific microbial functional traits remains insufficiently understood. This study addresses this gap by isolating two bacterial strains, Bacillus sp. ScRB44 and Pseudomonas sp. ScRB22, from the rhizosphere of the invasive weed Solidago canadensis. We assessed their nitrogen utilization capacity and indoleacetic acid (IAA) production capabilities to evaluate their ecological functions. Our three-stage experimental design encompassed strain promotion, nutrient stress, and competition phases. Bacillus sp. ScRB44 demonstrated robust IAA production and significantly improved the nitrogen utilization efficiency, significantly enhancing S. canadensis growth, especially under nutrient-poor conditions, and promoting a shift in biomass allocation toward the roots, thereby conferring a competitive advantage over native species. Conversely, Pseudomonas sp. ScRB22 exhibited limited functional activity and a negligible impact on plant performance. These findings underscore that the ecological impact of rhizosphere bacteria on invasive weeds is closely linked to their specific growth-promoting functions. By enhancing stress adaptation and optimizing resource allocation, certain microorganisms may facilitate the establishment of invasive weeds in adverse environments. This study highlights the significance of microbial functional traits in invasion ecology and suggests novel approaches for microbiome-based invasive weed management, with potential applications in agricultural soil health improvement and ecological restoration. Full article

Atherosclerosis is a chronic inflammatory disease and a major pathological basis of numerous cardiovascular conditions, with a high global mortality rate. Macrophages play a pivotal role in its pathogenesis through phenotypic switching and foam cell formation. Prostaglandin E2 receptor subtype 4 (EP4) highly expressed on the macrophage surface, is involved in various pathophysiological processes, such as inflammation and lipid metabolism. However, the role of macrophage EP4 in the progression of atherosclerosis remains unclear. To determine whether macrophage EP4 affects the progression of atherosclerosis by regulating foam cell formation and macrophage polarization. Myeloid-specific EP4 knockout mice with an ApoE-deficient background were fed a Western diet for 16 weeks. Our results showed that EP4 expression was significantly downregulated during atherosclerosis. EP4 deficiency was found to exacerbate atherosclerotic plaque formation and destabilizes plaques. In vitro studies further demonstrated that loss of EP4 in myeloid cells promoted foam cell formation and M1 macrophage polarization. Both transcriptomic and proteomic analysis showed that EP4 may regulate these processes by regulating CD36 expression in macrophage, which was further confirmed by Western blot and qPCR. In summary, deficiency of EP4 receptor in macrophages enhance foam cell formation and M1 polarization by upregulating CD36 expression, thereby accelerating the progression of atherosclerosis. Full article

Background: Obesity is characterized by a chronic state of low-grade inflammation. Investigating immune-critical genes and their biological functions in the adipose tissue of postmenopausal obese women is crucial for elucidating the underlying mechanisms of immune dysregulation associated with obesity. Methods: In this study, microarray (GSE151839) and single-cell RNA-seq (GSE176171) datasets were obtained from the Gene Expression Omnibus (GEO). For microarray data analysis, weighted gene co-expression network analysis (WGCNA), protein–protein interaction network (PPI) analysis, and immune infiltration analysis (ssGSEA) were employed to identify obesity-related immune-critical genes. Subsequently, the candidate genes were validated using scRNA-seq data to explore their expression patterns at the single-cell level. Finally, the expression levels of these immune-critical genes were experimentally verified in adipose tissue from obese and control zebrafish models using RT-qPCR. Results: Analysis of microarray data through WGCNA, PPI and ssGSEA identified 16 obesity-related immune-critical genes, including IL7R, CD3E, CD2, CCR5, CD3D, MS4A1, TRAT1, SLAMF8, CCL3L1, SPP1, CCL5, IL2RG, CD3G, TLR8, ITK, and CCL3. Differential expression of SPP1, ITK and CCL5 was confirmed in scRNA-seq data, with ITK and CCL5 showing distinct expression patterns in natural killer (NK) cells. Furthermore, RT-qPCR analysis revealed upregulation of SPP1 and ITK in adipose tissue of obese zebrafish compared to lean controls. Conclusions: This study identifies SPP1, ITK and CCL5 as key immune hub genes in the adipose tissue of postmenopausal obese women, with NK cells playing a significant role in adipose tissue inflammation through the expression of these genes. These findings provide novel insights into potential therapeutic targets for the prevention and treatment of obesity in postmenopausal women. Full article

Background: Increasing evidence shows that HCY plays an important role in stress-induced cognitive dysfunction, and HCY significantly promotes the decline of cognitive function. Stress has been reported to cause elevated HCY in the hippocampus of mice. Cognitive flexibility refers to the ability of individuals to quickly adjust their neurobehavioral strategies to different situations or to solve different tasks. Aims: This study aims to explore the role of HCY in the impairment of cognitive flexibility induced by stress and its possible regulatory mechanism. Methods and Results: First, we examined changes in the protein and mRNA levels of the cognitive flexibility effector molecule, PIN1, during stress in mice. The results show that stress can cause a decline in cognitive flexibility in mice and lead to an increase in PIN1. Moreover, through the use of in vitro experiments, we found that HCY could induce an increase in PIN1 expression in neurons. Further in vivo experiments were used to investigate the effect of VitB on HCY and PIN1 and evaluated the therapeutic effect of VitB on stress-induced impairment of cognitive flexibility. The results show that VitB decreased the levels of HCY in plasma and the hippocampus, alleviated the stress-induced impairment of cognitive flexibility, and reduced the expression of PIN1. Conclusions: These results suggest that the impairment of cognitive flexibility induced by stress can be inhibited by regulating the content of HCY. Collectively, our findings highlight therapeutic strategies aimed at improving HCY treatment for impairments in cognitive flexibility. Full article

Pre-lithiation using Li–Si alloy-type additives is a promising technical approach to address the drawbacks of Si-based anodes, such as a low initial Coulombic efficiency (ICE) and inevitable capacity decay during cycling. However, its commercial application is limited by the air sensitivity of the highly reactive Li–Si alloys, which demands improved environmental stability. In this work, a protective membrane is constructed on Li₁₃Si₄ alloys using low-surface-energy paraffin and highly conductive carbon nanotubes through liquid-phase deposition, exhibiting enhanced hydrophobicity and improved Li⁺/e⁻ conductivity. The Li₁₃Si₄@Paraffin/carbon nanotubes (Li₁₃Si₄@P-CNTs) composite achieves a high pre-lithiation capacity of 970 mAh g⁻¹ and superb environmental stability, retaining 92.2% capacity after exposure to ambient air with 45% relative humidity. DFT calculations and in situ XRD measurements reveal that the paraffin-dominated coating membrane, featuring weak dipole–dipole interactions with water molecules, effectively reduces the moisture-induced oxidation kinetics of Li₁₃Si₄@P-CNTs in air. Electrochemical kinetic analysis and XPS depth profiling reveal the enhancement in charge transfer dynamics and surface Li⁺ transport kinetics (SEI rich in inorganic lithium salts) in P-SiO@C pre-lithiated by Li₁₃Si₄@P-CNTs pre-lithiation additives. Benefitting from pre-lithiation via Li₁₃Si₄@P-CNTs, the pre-lithiated SiO@C(P-SiO@C) delivers high ICE (103.7%), stable cycling performance (981 mAh g⁻¹ at 200 cycles) and superior rate performance (474.5 mAh g⁻¹ at 3C) in a half-cell system. The LFP||P-Gr pouch-type full cell exhibits a capacity retention of 83.2% (2500 cycles) and an energy density of 381 Wh kg⁻¹ after 2500 cycles. The Li₁₃Si₄@P-CNTs additives provide valuable design concepts for the development of pre-lithiation materials. Full article

Non-glaucous wheat can reduce solar light reflection in low-light cultivation regions, enhancing photosynthetic efficiency and potentially increasing yield. In previous work, a non-glaucous cuticular line, YL-429, was discovered in derivatives of pentaploid hybrids by crossing the synthetic wheat LM/AT23 (non-glaucous cuticular) with its tetraploid donor parent LM (glaucous) and selfing to F₇ generations. In the present study, multicolor fluorescence in situ hybridization was used to characterize the karyotype of the YL-429 line; genome resequencing was performed to identify the breakpoint of the 2D-2A chromosome translocation of YL-429; and bulk sequencing analysis was conducted to detect the SNP in the translocated fragment and accordingly develop specific kompetitive allele-specific PCR markers for use in breeding. The line YL-429 was preliminarily determined as a 2DS and 2AS translocation (LM T2DS-2AS.2AL) line through karyotyping. Genome alignment identified an approximately 13.8 Mb segment, including the wax inhibitor gene Iw2, in the telomeric region of the 2DS chromosome arm replacing an approximately 16.1 Mb segment in that of the 2AS chromosome arm. According to the bulk DNA sequencing data, 27 specific KASP markers were developed for detecting the translocated fragment from the 2DS of Aegilops tauschii. The LM T2DS-2AS.2AL translocation line YL-429 could be helpful in improving the photosynthesis of durum wheat cultivated in low-light cultivation regions. The developed markers can assist the screening of the T2DS-2AS.2AL translocation in breeding. Full article

Environmental accounting information disclosure is crucial for heavily polluting enterprises to strengthen environmental governance and realize sustainable development. However, some enterprises still suffer from weak disclosure awareness and low disclosure quality. Therefore, improving the quality of environmental accounting information disclosure in the digital era has become an urgent task to achieve China’s goal of a green and low-carbon economy. Using data from Shanghai and Shenzhen A-share listed companies in China’s polluting industries from 2013 to 2022, this study explores the impact and channels of influence of digital transformation and environmental accounting information disclosure. It has been found that digital transformation significantly impacts the quality of environmental accounting information disclosure. Further, based on the dual perspectives of internal empowerment and external monitoring, digital transformation improves environmental accounting information disclosure by promoting executive compensation incentives and enhancing analyst attention. Furthermore, the positive impact of digital transformation on environmental accounting information disclosure is more pronounced with the implementation of new environmental protection laws, high-quality audits and a high level of digital transformation, and non-state-owned enterprises. The findings provide theoretical support for the government to improve the environmental accounting information disclosure system and provide valuable policy insights to promote digitalization and green, low-carbon transformation paths for heavily polluting enterprises. Full article

Micro-size light-emitting diodes (μLEDs) are high-brightness, low-power optoelectronic devices with significant potential in display technology, lighting, and biomedical applications. AlGaInP-based red LEDs experience severe size-dependent effects when scaled to the micron level, and addressing the fabrication challenges of GaN-based red μLED arrays is crucial for achieving homogeneous integration. This study investigates the employment of KOH wet treatments to alleviate efficiency degradation caused by sidewall leakage currents. GaN-based red μLED arrays with pixel sizes ranging from 5 × 5 µm² to 20 × 20 µm² were grown using metal-organic chemical vapor deposition (MOCVD), and then fabricated via rapid thermal annealing, mesa etching, sidewall wet treatment, electrode deposition, sidewall passivation, chemical-mechanical polishing, and via processes. The arrays, with pixel densities ranging from 668 PPI (Pixel Per Inch) to 1336 PPI, consist of 10,000 to 40,000 emitting pixels, and their optoelectronic properties were systematically evaluated. The arrays with varying pixel sizes fabricated in this study were subjected to three distinct processing conditions: without KOH treatment, 3 min of KOH treatment, and 5 min of KOH treatment. Electrical characterization reveals that the 5-min KOH treatment significantly reduces leakage current, enhancing the electrical performance, as compared to the samples without KOH treatment or 3-min treatment. In terms of optical properties, while the arrays without any KOH treatment failed to emit light, the ones with 3- and 5-min KOH treatment exhibit excellent optical uniformity and negligible blue shift. Most arrays treated for 5 min demonstrate superior light output power (LOP) and optoelectronic efficiency, with the 5 µm pixel arrays exhibiting unexpectedly high performance. The results suggest that extending the KOH wet treatment time effectively mitigates sidewall defects, reduces non-radiative recombination, and enhances surface roughness, thereby minimizing optical losses. These findings provide valuable insights for optimizing the fabrication of high-performance GaN-based red μLEDs and contribute to the development of stable, high-quality small-pixel μLEDs for advanced display and lighting applications. Full article

Modified atmosphere packaging (MAP) is commonly used to prolong the shelf life and maintain the quality of perishable food. However, it may contribute to more severe juice loss and texture changes in salmon. To explore the reasons why, this study designed different ratios of O₂ (to inhibit anaerobic bacteria), CO₂ (to inhibit Gram-negative bacteria), and N₂ (to maintain the packaging shape) in order to investigate the effects of MAP on the properties, structure, and oxidation of salmon proteins. The experiments’ results showed that MAP with about 60% CO₂ could slow bacterial growth effectively, as well as the accumulation of total volatile basic nitrogen and cooking loss. The carbonyl content decreased with increasing CO₂ contents but increased with high contents of O₂. A low concentration of O₂ (10%) was also beneficial for the inhibition of oxidation and degradation of proteins, and the lowest carbonyl content was found in 60%CO₂/10%O₂/30%N₂ conditions, with 2.01 μmol/g protein on day 12. Overall, we report that MAP with 60%CO₂ and 10%O₂ is properly able to limit structure changes in the myofibrils of salmon fillets during cold storage. Full article

Background/Objectives: Regadenoson, a selective adenosine A_2A receptor agonist, is primarily prescribed for myocardial perfusion imaging (MPI). As its clinical use becomes more widespread in practice, assessing its safety in real-world settings is essential. Methods: In this research, disproportionality analysis was applied to evaluate the safety of Regadenoson by examining all adverse event (AE) reports since 2004 in the FDA Adverse Event Reporting System (FAERS), in which Regadenoson was identified as the primary suspected drug. The reporting odds ratio (ROR), proportional reporting ratio (PRR), multi-item gamma Poisson shrinker (MGPS), and Bayesian confidence propagation neural network (BCPNN) were used to analyze AEs associated with Regadenoson. The Weibull distribution was utilized to model the temporal risk of AEs. Results: The results confirmed some known adverse reactions, such as nausea, shortness of breath (dyspnea), palpitations/vomiting, headache, dizziness, chest pain, and flushing (facial redness or warmth), which were also listed on the drug’s label. New potential adverse reactions not mentioned in the label were identified, including micturition urgency, mental status changes, conversion disorder, eye movement disorder, and genital paraesthesia. This study highlighted the significance of monitoring AEs, particularly right after the start of Regadenoson administration. Conclusions: This study provides preliminary safety data on Regadenoson’s real-world use, corroborating known adverse effects while uncovering new potential risks. These findings offer valuable safety insights for clinicians when prescribing Regadenoson for the use of MPI. Full article

cgr-miR166 was observed to be significantly enhanced in Chrysanthemum under 200 mM NaCl treatment. Here, ten family members were identified by aligning cgr-miR166 with scaffold sequences from the Chrysanthemum nankingense genome database, naming them from cgr-miR166a to cgr-miR166j, and their precursors could form stable stem-loop structures. The mature regions were observed to be highly conserved, with the 3′ end being more conserved than the 5′ end. miR166s promoters have been found to contain cis-acting elements responsive to diverse stimuli like the phytohormones ABA and IAA. qRT-RCR results demonstrated that the transcriptome sequencing results were reliable and miR166 was present at different levels in the roots, stems, leaves and flowers of Chrysanthemum. Furthermore, the HD-ZipIII transcription factor was validated to be the target gene of Chrysanthemum miR166s by degradome sequencing. Taken together, the cgr-miR166 family exhibited both evolutionary conservation and diversification. The expression level of miR166 was upregulated in root under salt stress, while the expression level of the target gene HD-ZipIII was downregulated. These findings established the foundation for further understanding the mechanism of miR166-HD-ZipIII modules in salt response and tolerance. Full article

With the rapid development of China’s economy, urban domestic sewage and industrial wastewater treatment efficiency has improved, resulting in a significant increase in sludge production. Thermal drying is essential for reducing, safely disposing of, and resourcefully utilizing sludge. However, this drying process inevitably releases harmful pollutants, posing potential environmental risks that necessitate careful management. This work focused on the thermal drying of municipal sludge at five temperature intervals (90–210 °C) and examined the impact of calcium oxide on sludge drying properties. The results indicated that higher temperatures increased sludge drying rates, with optimal efficiency achieved at a 15% calcium oxide addition. Online detection of NH₃, H₂S, CO, and CH₄ in the exhaust gas revealed that pollutant generation was temperature-dependent. While calcium oxide addition had no significant effect on CH₄ and CO emissions, it significantly inhibited the generation of H₂S and NH₃. This work provided crucial insights into optimizing sludge treatment, which improved drying efficiency and mitigated the release of hazardous pollutants, thereby reducing potential environmental and health risks associated with sludge disposal. Full article

This study utilizes historical monitoring data from the Xu Fan small watershed spanning 1962 to 2021 and employs the K-means clustering algorithm to classify extreme rainfall events into three distinct categories: short-duration high-intensity rainfall, sustained moderate-intensity rainfall, and long-duration heavy rainfall. Through the application of the Random Forest model, key factors influencing flood characteristics are identified, including total rainfall, maximum rainfall intensity, the timing of maximum intensity, and rainfall duration. The comparative analysis of data before and after 1990 highlights that climate change has led to increased maximum rainfall intensity, reduced rainfall duration, and shifts in the temporal distribution of rainfall, thereby exerting a significant influence on the flood generation process. Full article

To elucidate the mechanisms of ozone gas (OG) and slight acid electrolyzed water (SA) on the quality changes in texture, water-holding capacity, and softening of salmon, the bacterial growth, total volatile basic nitrogen, thiobarbituric acid reactive substance, a* value, texture properties, carbonyl content and free sulfhydryl content, myofibrillar fragmentation index, and proteolytic activities of salmon treated by OG (1 mg/m³ for 10 min) and SA (ACC 30 mg/L, 5 min) individually and in combination were studied. The results showed that total viable counts of SA + OG (dipped in SAEW for 5 min, followed by exposure to ozone for 10 min) was about 3.36 log CFU/g lower than the control (CK) (dipped in distilled water for 5 min) on day 10. Further studies indicate that at the end of storage, the hardness of SA + OG fillets only decreased by 33.95%, while the drip loss and myofibrillar fragmentation index (MFI) were the lowest (i.e., 14.76% and 101.07). The activity of cathepsin D was extensively inhibited by SA + OG, which was only 2.063 U/g meat at the end. In addition, the carbonyl content was 1.90 μmol/g protein, and the free sulfhydryl content was 39.70 mg/mL in the SA + OG group, indicating that protein oxidation was also effectively inhibited. Correlation analysis shows that bacteria and endogenous proteases are the main causes of protein degradation. Overall, the combination of OG and SAEW is an effective way to maintain the muscle quality of salmon by inhibiting bacterial growth and endogenous enzymes. Full article