Search Results (173)

Grapholita molesta is a globally significant fruit pest. Females achieve maximal reproductive output through efficient sperm utilization following a single copulation. Post-mating maturation of eupyrene sperm is a critical step in reproductive success. Here, we report that a male accessory gland-derived serine protease (named GmAGSP1) is essential for this process. GmAGSP1 was only distantly related to other identified sperm-activating SPs, and its transcript was highly expressed in the AG at 48 h after emergence. RNAi-mediated knockdown of GmAGSP1 in males did not affect courtship rate, copulation duration, or mating frequency, whereas male fertility decreased significantly. Mating with GmAGSP1-knockdown males markedly impaired eupyrene sperm maturation in the spermatophores, with phenotypes including failure of eupyrene sperm bundles to dissociate normally and marked reduction in viability of the dissociated eupyrene sperm. Finally, untargeted metabolomic analysis preliminarily demonstrated marked alterations in multiple metabolic pathways within the spermatophore following mating with GmAGSP1-knockdown males. This study advances our understanding of the regulatory mechanism of “sperm activation in the spermatophore’s metabolic microenvironment mediated by male AG-derived SP” while providing critical insights for the development of novel genetic control strategies targeting G. molesta. Full article

Pangong Tso, a typical plateau lake exhibiting an east-to-west gradient from freshwater to saline conditions, was used to simulate the migration and transformation of nitrogen compounds under different salinity conditions. This study systematically investigates the effects of salinity on nitrogen cycling and transformation in Pangong Tso sediments from 12 sites through controlled laboratory experiments and field monitoring across 120 sites. The data analysis method includes correlation analysis, ANOVA, structural equation modeling (SEM), and mixed-effects modeling (MEM). The results demonstrate that salinity significantly affects nitrogen cycling in plateau lakes. Salinity inhibits nitrification, resulting in an accumulation of ammonium nitrogen (NH₄⁺-N), while simultaneously suppressing gaseous nitrogen emissions through the inhibition of denitrification. Salinity has a significant negative effect on nitrite nitrogen (NO₂⁻-N), which is attributable to enhanced redox-driven transformations under hypersaline conditions. A salinity threshold of approximately 9‰ was identified, above which nitrite oxidation was strongly inhibited, consistent with the known high salinity sensitivity of nitrite-oxidizing bacteria (NOB). Higher salinity levels correlated positively with increased NH₄⁺-N and total nitrogen (TN) concentrations in overlying water (p < 0.01), and were further supported by observed increases in dissolved organic nitrogen (DON) and dissolved total nitrogen (DTN) along with rising salinity, and vice versa. Full article

Proton exchange membrane fuel cells (PEMFCs), recognized as promising sources of waste heat for space heating, domestic hot water supply, and industrial thermal applications, have garnered substantial interest owing to their environmentally benign operation and high energy conversion efficiency. Since the uniformity of oxygen diffusion toward catalytic layers critically governs electrochemical performance, this study establishes a three-dimensional, non-isothermal computational fluid dynamics (CFD) model to systematically optimize the cathode flow channel width distribution, targeting the maximization of power output through enhanced reactant homogeneity. Numerical results reveal that non-uniform flow channel geometries markedly improve oxygen distribution uniformity, reducing the flow inhomogeneity coefficient by 6.6% while elevating maximum power density and limiting current density by 9.1% and 7.8%, respectively, compared to conventional equal-width designs. There were improvements attributed to the establishment of longitudinal oxygen concentration gradients and we alleviated mass transfer limitations. Synergistic integration with gas diffusion layer (GDL) gradient porosity optimization further amplifies performance, yielding a 12.4% enhancement in maximum power density and a 10.4% increase in limiting current density. These findings validate the algorithm’s efficacy in resolving coupled transport constraints and underscore the necessity of multi-component optimization for advancing PEMFC design. Full article

FeSiBCuNb powders, produced via the gas–water atomization method, typically exhibit a broad particle size distribution and high sphericity. Nanocrystalline soft magnetic composites derived from these powders demonstrate exceptional service stability. In this study, a series of FeSiBCuNb/FeNi nanocrystalline magnetic powder cores (NMPCs) were fabricated under varying compaction pressures and heat treatment temperatures. The effects of these parameters on the soft magnetic properties were systematically analyzed. The findings reveal that optimizing compaction pressure and heat treatment temperature significantly enhances the density of the composite powders, leading to improved magnetic permeability and reduced core loss; when compaction pressure is 1800 MPa and heat treatment temperature is 550 °C, the NMPCs display outstanding magnetic properties with a low H_c of 6.32 Oe, high μ_e of 71.9, a low P_cv of 86.3 kW/m³ at 50 mT and 100 kHz, and 351.5 kW/m³ at 20 mT and 1000 kHz. Therefore, tailoring these processing conditions can enhance the soft magnetic performance of FeSiBCuNb nanocrystalline composites. Full article

Nanocrystalline powders, characterized by a biphasic amorphous nanocrystalline structure, demonstrate outstanding soft magnetic characteristics, including reduced coercivity (H_c), enhanced effective permeability (μ_e), and increased resistivity. However, their high hardness, poor formability, and significant core loss (P_cv) restrict their use in high-performance molded inductors. In this study, FeSiBCuNb/FeSiAl nanocrystalline soft magnetic composites (NSMCs) were fabricated, and the influence of varying the FeSiAl concentration on the microstructure, density, and soft magnetic characteristics of NSMCs was investigated. Then, the underlying mechanisms of these effects were explained. The results demonstrate that FeSiAl exhibits apparent deformation following compression, effectively filling the air gap between the FeSiBCuNb powder particles, thereby enhancing coupling among the magnetic particles. Consequently, the density of the NSMCs was enhanced, leading to a significant improvement in their overall soft magnetic properties. When 50 wt.% FeSiAl is added, the NSMCs display outstanding magnetic properties, including a low H_c of 4.36 Oe, a high μ_e of 48.7, a low P_cv of 119.35 kW/m³ at 50 mT and 100 kHz, and a high DC-bias performance of 73.29% at 100 Oe. Compared to NSMCs without FeSiAl, μ_e increased by 59.4% and P_cv decreased by 66.1%. Meanwhile, the incorporation of ultrafine FeSiAl powder was found to significantly improve the material properties, as the deformable FeSiAl particles effectively fill interparticle gaps during compaction, enhancing density and magnetic coupling. The 50 wt.% FeSiAl composition demonstrated exceptional properties. These advances address critical challenges in high-frequency power electronic applications and provide a practical material solution for next-generation power electronics. Full article

Forest bathing (Shinrin-Yoku in Japanese) is used as an intervention for improving mental health, with VR being used to create virtual forests for relaxation. Background/Objectives: In this research, we added therapeutic intent to a virtual forest with the goal of reducing social anxiety, with and without therapeutic instruction. Methods: Fifty-eight first-year psychology students were randomly assigned to one of three conditions: virtual forest only, therapeutic exercises only, and both combined. Results: All three conditions enhanced restorative effects equally. However, only the therapeutic exercise-only condition showed a tendency to reduce social anxiety. Participants in the combined condition reported more positive experiences and showed better comprehension of therapy content in the virtual forest. Conclusions: While the non-VR approach may offer immediate relaxation and possible anxiety reduction, combining the virtual forest with therapeutic exercises may yield better outcomes for sustained engagement and understanding over multiple therapeutic sessions. Full article

Layered sodium trititanate (Na₂Ti₃O₇) is a promising anode material for sodium-ion batteries due to its suitable charge/discharge plateaus, cost-effectiveness, and eco-friendliness. However, its slow Na⁺ diffusion kinetics, poor electron conductivity, and instability during cycling pose significant challenges for practical applications. To address these issues, we developed a template-free method to synthesize Na₂Ti₃O₇-C hollow microspheres. The synthesis began with polymerization-induced colloid aggregation to form a TiO₂–urea–formaldehyde (TiO₂-UF) precursor, which was then subjected to heat treatment to induce inward crystallization, creating hollow cavities within the microspheres. The hollow structure, combined with a conductive carbon matrix, significantly enhanced the cycling performance and rate capability of the material. When used as an anode, the Na₂Ti₃O₇-C hollow microspheres exhibited a high reversible capacity of 188 mAh g⁻¹ at 0.2C and retained 169 mAh g⁻¹ after 500 cycles. Additionally, the material demonstrated excellent rate performance with capacities of 157, 133, 105, 77, 62, and 45 mAh g⁻¹ at current densities of 0.5, 1, 2, 5, 10, and 20C, respectively. This innovative approach provides a new strategy for developing high-performance sodium-ion battery anodes and has the potential to significantly advance the field of energy storage. Full article

Black-odorous water (BOW) in urban areas poses significant risks to water safety and human health. Chemical oxygen demand (COD) is a critical parameter for the control and monitoring of BOW. However, traditional methods for COD determination are expensive, time-consuming, and involve the use of hazardous chemicals. In this study, reduced graphene oxide (rGO) and transitional metal particles (Cu, Ni) were used as working electrode materials for facile on-site determination of COD in BOW. Three composite materials (rGO/Cu, rGO/Ni, and rGO/Cu/Ni) were synthesized by one-step chemical reduction with different ratios, and their microstructure and chemical composition were characterized. Glucose solution and real water were used to evaluate the electrocatalytic performance of the different sensors. The ternary composite (rGO/Cu/Ni) screen-printed electrode sensor demonstrated excellent performance in COD analysis, with a low limit of detection (18.9 mg L⁻¹), a broad linear detection range from 53 to 1500 mg L⁻¹, and a 1.61% relative error for real water samples. The testing results were highly consistent with those obtained using the standard chromium sulfate method. This study offers promising prospects for the mass production of cost-effective COD electrochemical sensors, facilitating real-time, on-site monitoring of water bodies in major urban areas. Full article

In space gravitational wave detection, the phase information of interfering signals is read out by a phasemeter, typically output sampling at a MHz frequency. To transmit the phase information between space and ground, it must be downsampled; however, spectral aliasing during downsampling will affect the performance of the phasemeter.This paper presents a four-module cascaded downsampling filter (FCDF) with detailed module parameter design. On-board experiments conducted in a phasemeter environment demonstrate that the FCDF achieves a passband attenuation of less than $8.68\times {10}^{-6}$ dB and a stopband attenuation exceeding 160 dB, enabling downsampling from 80 MHz to 3.4 Hz. Additionally, the FCDF offers improved low-frequency noise suppression, which can enhance phasemeter performance. Full article

Freshwater fungi consist of a highly diverse group of organisms in freshwater habitats worldwide. During a survey of fungal diversity in freshwater habitats across different regions of Jiangxi Province, China, four freshwater fungi were collected. To study their phylogenetic relationships, the internal transcribed spacer (ITS1-5.8S-ITS2), large subunit (28S, LSU), small subunit (18S, SSU), and RNA polymerase II subunit (RPB2) genes were selected for phylogenetic analyses. Based on morphology coupled with phylogenetic analysis, these strains were confirmed to belong to Phaeoisaria, Pleurothecium, and Pseudodactylaria. Among them, three fungi were confirmed as the new species, namely, Pleurothecium lignicola (Pleurotheciaceae), Pseudodactylaria jiangxiensis (Pseudodactylariaceae), and Ps. lignicola (Pseudodactylariaceae). One species was identified as Phaeoisaria filiformis (Pleurotheciaceae), a new record of this species in China. All species were compared with other similar species, and detailed descriptions, illustrations, and phylogenetic data were provided. Full article

Toxoplasma gondii is a globally widespread pathogen of significant veterinary and medical importance, causing abortion or congenital disease in humans and other warm-blooded animals. Nevertheless, the current treatment options are restricted and sometimes result in toxic side effects. Hence, it is essential to discover drugs that demonstrate potent anti-Toxoplasma activity. Herein, we found that vorinostat, a pan-HDAC inhibitor, exhibited an IC₅₀ value of 260.1 nM against the T. gondii RH strain and a selectivity index (SI) > 800 with respect to HFF cells. Vorinostat disrupted the entire lytic cycle of T. gondii in vitro. Proteome analysis indicated that vorinostat remarkably perturbed the protein expression of T. gondii, and proteins involved in “DNA replication” and “membrane” were significantly dysregulated. Furthermore, we found that vorinostat significantly enhanced ROS production and induced parasite apoptosis. Importantly, vorinostat could prolong survival in a murine model. Our findings reveal that vorinostat is effective against T. gondii both in vitro and in vivo, suggesting its potential as a therapeutic option for human toxoplasmosis. Full article

Lichuan black tea (LBT) is a well-known congou black tea in China, but there is relatively little research on its processing technology. Echa No. 10 is the main tea tree variety for producing LBT. This study investigated the sensory quality, flavor components, and bioactivity of Echa No. 10 Lichuan black tea (LBT) at different drying temperatures (70, 80, 90, 100, 110, 120, and 130 °C). During 80–120 °C, increasing the drying temperature enabled a higher sweet aroma concentration and enhanced the sweetness in the taste, in contrast to reducing the floral, fruity, and sweet aromas, and increasing the bitterness and astringency, at >120 °C. Additionally, with an increasing drying temperature, the contents of tea polyphenols and total catechins significantly decreased, with the theaflavins decreasing first and then increasing, and the alcohols, aldehydes, esters, and hydrocarbons increasing first and then decreasing. Meanwhile, compounds (including linalool, (Z)-linalool oxide (furanoid), (E)-linalool oxide (furanoid), cis-β-Ocimene, and methyl salicylate) contribute more to the floral and fruity aromas at <110 °C. Furthermore, low-temperature drying favors the antioxidant and inhibitory effects of the α-amylase, α-glucosidase, and glucose absorption activity. Both the tea quality and bioactivity results revealed 80–110 °C as the optimal drying temperature range for LBT. Full article

To investigate the effect of combined end-and-shaft post-grouting on the vertical load-bearing performance of bridge-bored piles in the Dongting Lake area of Hunan, two post-grouted piles were subjected to bi-directional O-cell and top-down load tests before and after combined end-and-shaft grouting, based on the Wushi to Yiyang Expressway project. A comparative analysis was conducted on the bearing capacity, deformation characteristics, and load transfer behavior of the piles before and after grouting. This study also examined the conversion coefficient γ values of different soil layers obtained from the bi-directional O-cell test for bearing capacity calculations. Additionally, the characteristic values of the end bearing capacity, obtained from the bi-directional O-cell and top-down load tests, were compared with the values calculated using the relevant formulas in the current standards, which validated the accuracy of existing regulations and traditional loading methods. The results indicate that the stress distribution along the pile shaft differed between the two test methods. In the bi-directional O-cell test, the side resistance developed from the end to the head, while in the top-down load test, it developed from the head to the end. After combined post-grouting, the ultimate bearing capacity of the piles significantly increased, with side resistance increasing by up to 81.03% and end resistance by up to 105.66%. The conversion coefficients for the side resistance in silty sand and gravel before and after grouting are 0.86 and 0.80 and 0.81 and 0.69, respectively. The characteristic values of the end bearing capacity, as measured by the bi-directional O-cell and top-down load tests, were substantially higher than those calculated using the current highway bridge and culvert standards, showing increases of 133.63% and 86.15%, respectively. These findings suggest that the current standard formulas are overly conservative. Additionally, the measured values from the top-down load test may underestimate the actual bearing capacity of piles in engineering projects. Therefore, it is recommended that future pile foundation designs incorporate both bi-directional O-cell testing and combined post-grouting techniques to optimize design solutions. Full article

In this study, we analyzed the nutritional components and antioxidant capacity of 17 leaf mustard cultivars, including 13 cultivars from Leshan, 2 from Ya’an, and 2 from Yibin. Significant variations were observed among the cultivars in terms of their nutritional composition and antioxidant properties. Our findings revealed that cultivar LS12 exhibited the highest levels of total chlorophyll, total anthocyanidin, flavonoids, total phenolics, and ferric-reducing antioxidant power (FRAP). Cultivar YB2 showed higher contents of soluble sugar, soluble protein, ABTS antioxidant capacity, and total glucosinolates. On a regional basis, the cultivars from Leshan had higher total phenolics compared to those from Ya’an and Yibin, while cultivars from Yibin exhibited the highest total glucosinolate content. Additionally, purple-leaf mustard demonstrated superior flavonoid and total phenolic contents, as well as higher FRAP values, compared to the cultivars of green-leaf mustard. However, green-leaf mustard contained higher levels of total glucosinolates than their purple counterpart. Based on a comprehensive evaluation using a membership function analysis, LS12 was identified as the cultivar with the best nutritional quality and antioxidant capacity. This study provides valuable insights into the nutritional attributes and antioxidant capacity of different leaf mustard cultivars and offers guidance for selecting varieties to promote a healthy diet. Full article

Withering is one of the major processing steps critical for the quality of black tea. In this study, we investigated the mechanisms underlying the physicochemical changes in metabolites and gene expression during the withering process of black tea using metabolomic and transcriptomic approaches, respectively. Based on gas chromatography/mass spectrometry non-targeted metabolomic approaches (GC-MS) and ultra-high performance liquid chromatograph–tandem mass spectrometry (UHPLC-MS/MS), a total of 76 volatile compounds and 160 non-volatile compounds were identified from tea leaves, respectively. RNA-seq analysis revealed that the number of differentially expressed genes (DEGs) for the comparative combination of withering time (i.e., W4h, W6h, W8h, W10h, and W12h) compared with CK (i.e., fresh leaves) were 3634, 2906, 4127, 5736, and 7650, respectively. The core genes in starch metabolism, namely alpha-amylase (AMY) and beta-amylase (BAM), were upregulated as withering time increased. AMY and BAM contributed to the decomposition of starch to increase the soluble sugars. The content of tea leaf alcohols and aldehydes, which are the vital contributors for greenish aroma, gradually decreased as withering time increased due to the downregulation of associated genes while the compounds related to sweet and fruity characteristics increased due to the upregulated expression of related genes. Most DEGs involved in amino acids were significantly upregulated, leading to the increase in free amino acids content. However, DEGs involved in catechins metabolism were generally downregulated during withering, and resulted in a reduction in catechins content and the accumulation of theaflavins. The same trend was observed in alpha-linolenic acid metabolism-related genes that were downregulated and enhanced the reduction in grassy aroma in black tea. The weighted gene co-expression network analysis (WGCNA) of DEGs showed that one module can be associated with more components and one component can be regulated by various modules. Our findings provide new insights into the quality formation of black tea during the withering process. Full article