Bioprospecting Plant Growth Promoting Rhizobacteria for Enhancing the Biological Properties and Phytochemical Composition of Medicinally Important Crops

Abstract

Traditionally, medicinal plants have long been used as a natural therapy. Plant-derived extracts or phytochemicals have been exploited as food additives and for curing many health-related ailments. The secondary metabolites produced by many plants have become an integral part of human health and have strengthened the value of plant extracts as herbal medicines. To fulfil the demand of health care systems, food and pharmaceutical industries, interest in the cultivation of precious medicinal plants to harvest bio-active compounds has increased considerably worldwide. To achieve maximum biomass and yield, growers generally apply chemical fertilizers which have detrimental impacts on the growth, development and phytoconstituents of such therapeutically important plants. Application of beneficial rhizosphere microbiota is an alternative strategy to enhance the production of valuable medicinal plants under both conventional and stressed conditions due to its low cost, environmentally friendly behaviour and non-destructive impact on fertility of soil, plants and human health. The microbiological approach improves plant growth by various direct and indirect mechanisms involving the abatement of various abiotic stresses. Given the negative impacts of fertilizers and multiple benefits of microbiological resources, the role of plant growth promoting rhizobacteria (PGPR) in the production of biomass and their impact on the quality of bio-active compounds (phytochemicals) and mitigation of abiotic stress to herbal plants have been described in this review. The PGPR based enhancement in the herbal products has potential for use as a low cost phytomedicine which can be used to improve health care systems.

1. Introduction

Medicinal plants, also called medicinal herbs, have been identified and herbal extracts have been used/practiced as traditional medicines since prehistoric times. Among herbal medicine practicing nations, India has a rich reserve of valuable medicinal plants due to its varying geographical conditions and climate diversity [1]. Almost all parts, including roots, stems, leaves and seeds, of medicinal plants have therapeutic potentials and can be used to treat different infections [2,3]. In addition, medicinal plants secrete several essential secondary metabolites or marker compounds having medicinal value that can be used for curing and preventing human illnesses [4,5,6]. Medicinal plants, in addition to their role in disease management, can also be used in cosmetics industries and other health care products to satisfy the user’s demands [7,8]. Due to this, medicinal plants are cultivated worldwide for both therapeutic purposes and to fulfil the demands of domestic industries. The World Health Organization (WHO) estimates that nearly 80% of the global population presently uses traditional herbal medicines for some aspect of primary health care [9]. In developing countries such as India, about 80% of the total population depends on conventional phytomedicines [10]. The traditional or classical medicines are widely used in many countries including India, China, Japan, Pakistan, Sri Lanka and Thailand. Thus, the interest in the development and recognition of the medicinal value of such an important group of plants in both industrialized and developing countries is increasing continuously [7]. The aromatic constituents and secondary metabolites such as flavonoids, polyphenols and other classes of phytochemicals are the two most important phytoconstituents that play important roles in treating various human diseases [11]. Due to this, the traditional herbal medicines are used throughout the world as an alternative to modern day medicines [12]. For instance, medicinal plants have been found effective in combating many human diseases including cancer [13] and other viral diseases such as AIDS, hepatitis, etc. [14,15,16]. The traditional plant-based medicines, therefore, occupy a significant place in the modern-day drug industries because they are safe, inexpensive and pharmacologically better compatible. In addition, the combination of various plant secondary metabolites has shown synergistic action and, hence, they are doubly effective [17]. Besides these, the compounds and metabolites secreted by herbal plant genotypes protect the producing plants from attack by microbes and insect pathogens [18,19].

Due to various therapeutic potentials and the presence of variable phytochemicals, medicinal plants hold a special significance in sustainable agriculture systems [20]. Therefore, the demand for cultivation of medicinal plants is steadily increasing worldwide. Hence, the focus has currently been shifted to the cultivation of different therapeutically useful medicinal plants such as ashwagandha, opium, aloe, isubgol, aonla, medicinal solanum, stevia, etc. using sustainable approaches [21]. To achieve these, chemical fertilization is practiced in the cultivation of herbal plants which, however, due to challenges such as cost and harmful impacts on the biomass, yield and valuable metabolites are avoided in medicinal plant production systems [22]. The application of low-cost natural resources and beneficial microbiological formulations (biofertilizers), especially those prepared from PGPR group organisms, either alone or in synergism with other agronomically beneficial microbiota, in this regard have been reported to optimize the production of good quality medicinal plants under both conventional and stressful conditions without depending on synthetic fertilizers [23,24,25]. Recently, the commercialization of various PGPR formulations has emerged as an inexpensive and non-hazardous microbiological tool to minimize the use of chemical input in the production of medicinal plants [26,27]. Acknowledging the importance of medicinal plants and herbal extracts in human health and the role of the microbiome in the yield optimization, the present review summarizes the current knowledge available on the mechanistic basis of PGPR in enhancing the quantity and quality of biomass, yield and bio-active compounds/phytochemicals of different important medicinal plants growing under normal and problem soils. The bio-antimicrobials secreted by different medicinal plants provide a low cost, ecofriendly and sustainable option to the public to improve health care systems.

2. Secondary Metabolites of Medicinal Plants: Importance for Human Health

Medicinal plants synthesize some specialized compounds or secondary metabolites, referred to as natural products, a rich source for modern pharmaceuticals and drug-based industries [28]. Such phytochemicals with therapeutic potentials play numerous important roles in maintaining the versatility of pharmacological effects. Based on their role in various metabolic processes, the phytochemicals have been grouped as primary and secondary metabolites. Of these, the primary metabolites affect basic physiological functions, whereas the secondary metabolites have the pharmacological effects. The secondary metabolites have also been found effective in disease management, which establishes their use in traditional medicines. In modern medicine, the secondary metabolites provide essential antimicrobial compounds needed for treating various diseases [29].

The bioactive compounds significantly influence the healthcare, food and cosmetics industries in terms of value addition and preservation [30,31]. The secondary metabolites play many crucial roles, for example, they: (i) act as a carbon resource for plants, (ii) defend plants against pathogen attack [32,33], (iii) impart colour to flowers which attracts pollinators and (iv) protect plants from harmful UV radiations [34]. Plant–environment interactions, their survival and propagation are largely dependent on the secondary metabolites of plants and, therefore, the plethora of different phytochemicals greatly affects the survival of plants [35,36]. Apart from these, the microbial and parasitic infections can effectively be cured by the extracts derived from several medicinal plants [37]. For instance, several antifungal proteins such as glucanase, chitinase, etc. and other non-enzymatic proteins have been found within the seeds of medicinal plants, which protects the developing embryo from many infections [38,39]. Shoemaker et al. [40] report in a study that over 400,000 species of plants exist on earth which possess huge reservoirs of bioactive compounds, but unfortunately, only a small fraction of these biomaterials has been explored for research studies. Following screening of several traditional medicinal plants, it was recorded that the plant-derived bioactive compounds exhibited a substantial number of therapeutic properties. Consequently, a large number of clinically important plant-derived compounds (bio-antimicrobials) are available today, which are used for the production of several antitumoural [41], antifungal [42,43] and anticancer drugs [44]. Due to the emergence of resistance among pathogens against various drugs and the adverse effects of modern medicines on human health, the medicinal plants and the compounds derived from them have received greater attention globally due both to their antimicrobial properties and almost total lack of side effects [45]. Recently, the essential oils of medicinal plants, for example, Callistemon lanceolatus, Ocimum gratissimum, Cymbopogon winterianus Jowit, Cymbopogon flexuosus, Mentha longifolia and Vitex negundo were reported to have variable inhibitory impacts on Gram positive (Staphylococcus aureus, Micrococcus luteus and Bacillus subtilis) and Gram negative (Escherichia coli and Klebsiella pneumoniae) bacteria, due to differences in their antioxidant and antimicrobial activities. Moreover, the essential oils increased the antibacterial and antioxidant efficiency of other compounds and caused a substantial decline in active doses when used in combination [46]. Furthermore, the phytocompounds, such as protocatechuic acid, 3,4-dihydroxyphenylacetic acid, (+)-catechin, chlorogenic acid, 4-hydroxybenzoic acid, caffeic acid, vanillic acid, syringic acid, 3-hydroxybenzoic acid, verbascoside, p-coumaric acid, ferulic acid, hesperidin, hyperoside and rosmarinic acid have been extracted from Camellia sinensis, Erica arborea, Ilex paraguariensis, Rosemarinus officinalis and Thymus vulgaris [47]. The extract derived from the rhizomes of rose plants, for example, acts as a stimulant. Rose root possesses many pharmacological and therapeutic properties. Salitroside tyrosol, cinnamic acid and glycosides (rosavin, rosarin, rosin) are some of the useful bio-active compounds derived from rose plants [48,49]. Drosera is yet another plant that produce various secondary metabolites, such as the most common naphthoquinones [50]. Similarly, the leaves and berries of bilberry (Vaccinium myrtillus) plants contain significant amounts of phenolic compounds [51]. Bilberries also secrete exceptionally high amounts of anthocyanins, which have strong antioxidant potential. The bilberry extract has shown promising results in the treatment of cancer and cardiovascular diseases. The flavonoids of bilberry plants also possess antimicrobial activities (antiviral, antibacterial and antifungal) and antiallergenic properties. Furthermore, the juice of cranberry can preclude urinary tract infections (UTI) in women [52]. The bio-efficacy of such metabolites, however, varies from plant genotype to genotype, source of extraction and chemical composition (

Table 1. Secondary metabolites synthesized by some important medicinal plants.

S. No.	Medicinal Plant	Secondary Metabolites/Bioactive Compounds					Ref.
1.	Mentha piperita (peppermint)	Hesperidin	Diosmin	Didymin	Rosmarinic acid		[53]
2.	Aloe vera (aloe)	Aloin Anthraquinones	Lupeol Cinnamic acid Salicylic acid		β-sitosterol or campestrol Fatty acids		[54,55]
3.	Ocimum sanctum (holy basil)	Eugenol	Carvacrol	Rosmarinic acid	Oleanolic acid		[56]
4.	Datura inoxia (pricklyburr)	Terpenoids Phenols	Flavonoids		Saponins		[57]
5.	Dracocephalum moldavica (Moldavian balm or dragonhead)	Phenolic acids (p-coumaric acid)	Chlorogenic acid		Ellagitannins (apigenin)	Flavonoids (gentisic acid)	[58]
6.	Piper nigrum (black pepper)	Piperic acid Piperine		Piperttine Piperamide			[59]
7.	Withania somnifera (ashwagandha)	Withanine		Somniferine Withanolides			[60,61]
8.	Zingiber officinale (ginger)	Geranial	Phenylpropanoid	Oleoresin Gingerols			[62]
9.	Trigonella foenum-graecum (fenugreek)	Coumarins Flavonoids	Polyphenols	Steroidal sapogenins (diosgenin) Alkaloids (protodioscin)			[63,64]
10.	Papaver Somniferum (opium poppy)	Morphine	Narceine	Thebaine	Papaverine		[65,66]
11.	Ocimum basilicum (sweet basil)	Various phenolic compounds					[67]
12.	Cuminum cyminum (cumin)	Coumarins	Anthraquinone	Steroids	Flavonoids		[68]
13.	Curcuma longa (turmeric)	Terpenoids	Flavonoids	Phenols	Curcumin		[69,70]

).

3. Plant Growth Promoting Rhizobacteria Influencing the Cultivation of Medicinal Plants

The medicinal plant growers adopt various cultivation practices such as rotation of crops, use of varieties resistant to pathogens and chemical fertilizers, etc., to optimize their production [71]. Largely, the growers have focused on the conventional practices of fertilization while the scientific community or progressive farmers have shown interest in the use of bioformulations to optimize the safe production of phytochemicals and their medicinal value. However, due to the undesirable impact of chemical fertilizers and emergence of resistance among pathogens [72,73], there is an urgent need to reduce the use of agrochemicals in cultivation practices for augmenting the yield of medicinal plants and maintaining the quality of secondary metabolites. However, there are few studies which have reported the use of low cost and environmentally non-hazardous microbial communities in the production of medicinal plants and protection/alleviation of abiotic stresses [74,75]. Due to this, the use of economical, environmentally friendly and sustainable alternatives such as those of plant growth promoting rhizobacteria have been highlighted.

3.1. Direct Mechanisms

3.1.1. Biological Nitrogen Fixation (BNF) and P-Solubilisation

Nitrogen, the most vital plant nutrient, can be supplied to plants by free living bacteria such as Azotobacter, associative bacterium Azospirillum or endophytic bacteria Gluconoacetobacter diazotrophicus [76,77]. Some of the other notable nitrogen-fixing PGPR are Herbaspirillum, Bacillus, Burkholderia, Paenibacillus, etc. [78]. Free-living nitrogen-fixing bacteria form a very close association with plants without penetrating the tissues and maintain sufficient numbers to supply available N to growing plants. Phosphorus, next only to N, is the other major plant nutrient which affects different growth and developmental processes such as photosynthesis, respiration, signal transduction and energy transfer of plants [79]. Various plant growth promoting rhizobacteria associated with insoluble P to soluble P transformation, and generally called phosphate solubilizing microorganisms (PSM), benefit plants by supplying P. The PSM inhabiting rhizospheres/phyllospheres/endospheres include bacteria, fungi and actinomycetes which, through different mechanisms, makes P available to plants [80,81]. The most widely accepted mechanism of P solubilisation by microbes in general includes the release of organic acids, OH⁻ ions, CO₂ and protons. Some of the important P-solubilizing genera are Pseudomonas [82], Bacillus [83], Burkholderia [84], etc.

3.1.2. Production of Phytohormones

Indoleacetic Acid, Cytokinins and Gibberellins

The phytohormone indole-3-acetic acid (IAA) is one of the major plant hormones excreted by more than 80% of the rhizosphere microbiomes [85,86], such as Bacillus sp. and Enterobacter sp. [87], which controls different physiological process of plants—(i) organogenesis, (ii) cellular activities such as growth, division and differentiation, (iii) gene regulation and (iv) responses to environmental variations for instance, light and gravity [88]. Moreover, the plants receiving IAA for extended periods have well-developed root systems which benefit the plants to absorb maximum nutrients and minerals from soils [89]. Cytokinins, another important phytohormone secreted by PGPR, are physiologically identical to IAA [78] and control cell division and the cell cycle in addition to activating many plant developmental processes [90]. The major stimulatory or inhibitory activity of cytokinin includes regulation of growth (root and shoot) and branching, control of shoot apical dominance, development of chloroplasts and controlling the relocation of nutrients from leaf to reproducing seeds [91]. In addition, cytokinin alters the size and activity of meristems through cell division activity of embryonic and mature plants [92]. Gibberellins (GAs) are the other group of important phytohormones that stimulate many metabolic events such as germination, flowering, stem elongation and fruit formation [93]. The synthesis of gibberellins among the microbiome is generally uncommon. However, species of Bacillus, for example, B. licheniformis and B. amyloliquefaciens, have been reported as gibberellin producers [94].

3.2. Indirect Mechanisms

3.2.1. Aminocyclopropane-1-carboxylic Acid (ACC) Deaminase

ACC deaminase among plant growth modulators is a significant growth regulator that modifies the biochemical reactions and influences the development of plants indirectly by decreasing the level of stress hormone, ethylene [95,96]. A divergent group of plant growth promoting rhizobacteria secretes ACC deaminase which splits ACC into ammonia and α-ketobutyrate and thus limits its conversion to ethylene. Bacteria synthesizing ACC deaminase belongs to genera Pseudomonas [97], Bacillus [98], Acinetobacter [99], Azospirillum [100], Achromobacter [101], Enterobacter [102], Burkholderia [103], Agrobacterium [104], Rhizobium [105], Serratia [106], etc.

3.2.2. Release of Siderophores, Cyanogenic Compounds and Ammonia

Siderophores are small molecular weight (≈200–2000 Daltons) compounds secreted by plant growth promoting bacteria [107,108], for example, Bacillus [109,110], Pseudomonas [111,112], etc., which can act as antagonists under iron-deficient conditions because they make iron inaccessible for uptake by harmful soil-borne phytopathogens. Siderophores thus control soil-borne phytopathogens by limiting iron availability [113] and, therefore, destroy their growth and disease-causing potential [114]. Among the different types of siderophores secreted by bacteria, pyoverdin, produced generally by pseudomonads [115], impedes the growth of phytopathogenic bacteria and fungi [116]. Pseudobactin siderophore, excreted by the P. putida strain B10, in contrast has been reported to destroy the growth and disease-causing ability of Fusarium oxysporum by restricting the iron supply. Collectively, the secretion of siderophores is one of the most important biological components of disease management and an indirect way of enhancing growth by siderophore positive PGPR. Apart from siderophores [117], different antibacterial compounds [118,119] produced by PGPR play important roles in preventing the damage caused by phytopathogens and concurrently augment the yield of crops.

The production of volatile cyanogenic substances such as hydrogen cyanide (HCN) through cyanogenesis by living organisms including PGPB [120,121] controls various plant diseases caused by bacterial or fungal phytopathogens, thereby assisting the plant growth promotion [122]. Mechanistically, HCN secreted generally by species of Pseudomonas [123] and Bacillus adversely affects the growth and spread of pathogenic microbes by inhibiting the transfer of electrons and supply of energy to the bacterial cells. For example, HCN positive bacterial strains have been found to have beneficial effects on seedling root growth of various plants by restricting the growth of phytopathogens [124]. In addition to producing cyanogens, many bacteria possess ammonia (NH₃) secreting ability, which is also considered a unique plant growth enhancing feature [125,126].

3.2.3. Secretion of Antibiotics and Lytic Enzymes

Synthesis and release of antibiotics by PGPR is yet another important antagonistic trait which they use to manage the specific phytopathogens [127,128]. Studies by different workers have proved that the diffusible antibacterial drugs such as pyrrolnitrin, phycocyanin, 2,4-diacetylphloroglucinol (DAPG), etc. by different PGPR resulted in the suppression of phytopathogens [129,130]. Mechanistically, the antibiotics impair the integrity of the membranes and formation of initiation complexes on the small subunit of the ribosome [131]. As an example, the 2,4-diacetylphloroglucinol, an active and widely studied antibiotic produced by several strains of pseudomonas spp. has been found to damage the plasma membrane, alter vacuolization and disintegrate the cellular contents of Pythium ultimum var. Sporangiiferum sp., and it concurrently inhibited the zoospore formation [132]. Similarly, phenazine secreted by pseudomonas bacteria exhibits redox activity which can control the populations and infective abilities of F. oxysporum and Gaeumannomyces graminis [132]. Other important antibiotics are polymyxin, circulin and colistin, produced by strains of Bacillus which inhibits the growth of pathogenic bacteria and fungi [133]. Secretion of a series of cell wall degrading enzymes such as chitinases [134], glucanases [135], β-1,3-glucanases [136], cellulases [137], proteases [138], lipases [139], etc. by PGPR are the other factors that contribute in controlling the damage caused by microbial phytopathogens (Figure 1).

4. Rhizobacteria Mediated Improvement in Growth and Phytochemicals of Selected Medicinal Plants: Inoculation Effects

Although PGPR have shown tremendous increase in the biological and chemical features of many food and industrial crops [140,141,142], the data on the beneficial impact of PGPR on the performance of medicinal plants are relatively scarce [143,144,145] because very few greenhouse/field experiments targeting specifically PGPR–medicinal plant interactions have been conducted. Further, the mechanisms/pathways concerning the synthesis of secondary plant metabolites have not yet been fully understood, but the PGPR may also interact with medicinal plants in ways similar to those adopted by other crops. Therefore, an effort is directed herein towards understanding the role of PGPR in the growth and yield optimization of medicinal plants, citing some relevant examples. The inoculation of PGPR enhances the quality of the bioactive compounds of medicinal plants (Figure 2), which eventually benefits the pharmaceutical industries [146,147].

4.1. Datura

The genus Datura, often touted as “thorn apple”, belonging to the family Solanaceae with nine different species [148] has narcotic and medicinal values [149,150]. Additionally, ingestion of datura extracts or plant parts may cause severe poisoning or hallucinations [151]. The tropane alkaloids viz. hyoscyamine, atropine and scopolamine are the toxic components of datura [152,153]. Of these, atropine and scopolamine possess anticholinergic activities which are used in very low doses. Furthermore, scopolamine is an antimuscarinic agent and is a good muscle relaxant. It also possesses anti-nauseant properties and is a known antispasmodic agent which is used for treating motion sickness and in medication before operation [154]. Some of the other human health problems that may arise due to the ingestion of atropine includes blurred vision, reduced salivation, vasodilation and increased heart rate [155]. It can also be used as an antidote to organophosphorus insecticides [156]. Despite the impressive medicinal value and realization of the detrimental impact of chemical fertilization, few studies focusing on PGPR-Datura interactions have been reported [157,158]. The application of biofertilizer consisting of a mixture of nitrogen fixers (Azotobacter chroococcum and Azospirillum brasilense) and phosphate dissolving bacteria (Bacillus megaterium var. phosphaticum) along with mineral fertilizers (N and P) under field conditions demonstrated the best vegetative (plant height, number and total area and dry matter accumulation in leaves) and reproductive growth, mineral status, yield and quality of seeds and leaf anatomy [157,158]. In addition, PGPR enhanced the concentration of tropane alkaloids of thorn apple. Importantly, the combination of 50% NP mineral fertilizer and biofertilizer produced the best results compared to 100% NP mineral fertilizer when applied alone. Thus, the application of PGPR reduced the level of mineral fertilizer in datura cultivation, which is considered safer and more economical and can also minimize the environmental pollution caused by repeated application of mineral fertilizers [159]. To further strengthen the role of PGPR, Rahmoune et al. [147], in an independent greenhouse experiment, observed that the inoculation of beneficial PGPR such as Pseudomonas plecoglossicida, Lysinibacillus fusiformis and Bacillus sp. dramatically increased the growth and development of datura plants. Furthermore, the C/N ratio of plants was increased substantially. The alkaloid production in D. stramonium leaf, however, did not differ significantly, but its composition was altered in shoots following PGPR inoculation. Besides alkaloids, PGPR can also influence the composition and concentrations of amino acids. The amino acids and their derivatives detected in roots or in foliage of D. stramonium by gas chromatography–electron impact/Time-of-flight Mass spectrometry (GC–EI/TOF MS) included alanine, alanine 3-cyano-, alanine beta-, arginine, asparagine, aspartic acid, butanoic acid 4-amino-, butyro-1,4-lactam, glutamic acid, glutamine, glycine, histidine, homocysteine, homoserine, isoleucine, leucine, lysine, ornithine, ornithine-1,5-lactam, phenylalanine, proline, pyroglutamic acid, serine, threonine, tryptophan, tyrosine and valine [160].

4.2. Aloe vera

Aloe vera (Aloe barbadensis Miller) is a perennial plant belonging to family Liliaceae. The juice extracted from A. vera is effective in relieving digestive problems. A. vera also serves as a bio-antimicrobial and a skin protectant against harmful ultraviolet (UV) rays [161,162,163]. Other medicinal applications of A. vera include increased blood flow to wounded areas and enhanced production of fibroblasts. Certain liver related problems, gastric problems, inflammatory bowel disease, etc., can also be cured by A. vera [164]. In the form of gel, A. vera is used for topical (wounds, burns, skin irritations, etc.) as well as for internal applications (for treating constipation, coughs, ulcers, diabetes, headaches, arthritis, etc.) [165,166]. In order to optimize the biomass production and to improve phytochemical quality of A. vera, PGPR, either alone [167] or in combination with other biofertilizers such as PSB or mycorrhizal fungi, have been used [168]. For example, an increase in aloin content of A. vera following single or co-inoculation of rhizobacteria Azospirillum, Azotobacter, Bacillus and Pseudomonas has been reported [169]. Mamta et al. [170] in an identical experiment recorded a noteworthy upsurge in root length, leaf length and number of foliage, volume and dry weight of gel and whole plants when bio-primed with Pseudomonas synxantha, Serratia marcescens, Burkholderia gladioli and Enterobacter hormaeche. In addition, the aloin-A content of A. vera plants was increased due to PGPR application.

4.3. Withania somnifera (Ashwagandha)

Withania somnifera (Ashwagandha), a member of family Solanaceae, having several medicinal properties, has been used as a remedy in the ancient system of Indian medicine [171,172]. The underground (roots) parts of this plants are dried and are used to cure various nervous and sexual disorders. The drug obtained from Ashwagandha contains many biologically active compounds termed as ‘Withanolides’ [173]. Among various withanolides, Withaferin-A is therapeutically active and is found in the leaves and roots of plants. Withaferin-A has significant anticancer properties [174]. This plant can also be used as a potent anti-stress adaptogen, a potent antimicrobial agent and in the treatment of hypothyroidism [175,176]. The monumental medicinal value prompted Rajasekar and Elango [177] to grow this plant using a mixture of Azospirillum, Azotobacter, Pseudomonas and Bacillus. These PGPR either alone or in combination considerably augmented the height and length (root) of plants and alkaloid content of inoculated W. somnifera plants. In a follow up greenhouse production system, the growth and yield of W. somnifera was improved substantially following single or dual inoculation of B. subtilis, P. fluorescens, A. chroococcum, A. brasilense, Methylobacterium radiotolerans, Exiguobacterium acetylicum, Paenibacillus polymyxa, Pantoea dispersa and B. sonorensis [178]. The PGPR applications, in general, substantially enhanced the dry matter accumulation in root and shoot and whole plants, N and P in root and shoot, respectively, Withaferin-A concentration in roots and total withanolide content in plants relative to control. Among all PGPR, B. sonorensis, however, had significantly maximum beneficial impact on all the measured biological factors such as height, girth, root and shoot dry weight of plants and withanolide concentration [178]. The increase in the plants’ activity was attributed to inoculation of B. sonorensis, which possessed several PGP activities such as P solubilisation, IAA secretion, siderophore, amylase, ammonia and HCN production. These growth regulators together contributed to the maximum enhancement in the plant biomass and phytochemical production of W. somnifera plants [178].

4.4. Fenugreek

Fenugreek (Trigonella foenum-graecum L.) is a precious herbal plant which is spread worldwide. It belongs to the family Fabaceae and, apart from being grown as a medicinal plant [179,180], it is cultivated both as a spice and vegetable crop. The anti-atherosclerotic properties of fenugreek have been well documented [181]. Fenugreek also has some nutritional importance, for example, its seeds are full of compounds such as diosgenin, tannic acid, trigocoumarin, trigonelline, alkaloids, trigomethyl coumarin, gitogenin, vitamin A, etc. [64,182], whereas its foliage is rich in iron, calcium, β-carotene and other essential vitamins, which suggests that fenugreek has both nutritional and medicinal value [183].

Plant growth promoting rhizobacteria, when applied under cultivation practices, have displayed beneficial effects on the overall performance of medicinal plants. For this reason, Sharghi et al. [184] applied biofertilizers Rhizobium meliloti, P. fluorescens and a combination of both bacterial cultures to assess their impact on physiological and morphological characteristics of fenugreek cultivated under drought conditions. The results showed a significant increase in foliage area, fresh and dry biomass of shoot and root, P and K concentration, and water use efficacy (WUE) following inoculation with single or a mixture of PGPR. The individual PGPR formulation was, however, more effective compared to dual application while seed yield decreased in PGPR treated plants. In a follow up study, the co-inoculation impact of Sinorhizobium meliloti and P. fluorescens on the growth of fenugreek plants was evaluated under varying soil water levels. The S. meliloti and P. fluorescens applied together with 100% soil water level maximally increased the seed weight per plant. However, when the soil water level was 40%, the inoculated PGPR strains maximally enhanced the nicotinic acid and trigonelline of fenugreek plants. The applied PGPR strains showed positive/beneficial impacts on the morphological, physiological and phytochemical characteristics of fenugreek plants [185].

4.5. Turmeric

Turmeric (Curcuma longa L.), a rhizomatous herbaceous perennial plant, belongs to family Zingiberaceae. Turmeric is widely used as a spice crop, though it possesses tremendous medicinal value and, therefore, is widely used as a traditional or folk medicine [186]. Turmeric plants have been used to treat different diseases such as asthma, bronchial hyperactivity, rheumatism, diabetic wounds, sinusitis, smallpox, skin cancer, urinary tract infection and liver ailments, etc. [187]. Moreover, it has also been found effective against jaundice, abdominal pain, etc. [181]. The pharmacological traits such as anti-inflammatory, antioxidant, antimalarial, anticancer, hypolipidemic and immune-enhancer properties of turmeric are all attributed to curcumin, which is a yellow-coloured substance derived from the plant and is an essential ingredient of curcuminoids [182,183]. The chemical compound ‘curlone’ of turmeric has ROS scavenging (antioxidant) and antimutagenic activities [184].

Chemical fertilization such as with N, P and K is a common agronomic practice to optimize turmeric production worldwide [185,186]. The long term and random application of synthetic fertilizers, however, hampers the production of turmeric and alters the texture and nutrient pool of soil. Therefore, the use of PGPR across different physiological groups seems highly beneficial in the context of growth and yield of turmeric and the fertility of soil. In this regard, turmeric rhizomes interact with variously distributed soil microbial communities both as rhizospheres and as endophytes [187,188]. In general, the conventional rhizosphere microflora and microbial communities colonizing inside plant tissues (endophytic species) modulate the morpho-anatomical growth, secretion of secondary metabolites, curcumin content, antioxidants and biotic stress management [187,189,190,191,192,193,194,195]. The inoculation of turmeric plants with rhizobacterial cell suspensions containing Pseudomonas and Bacillus sp. (1:1) significantly enhanced the yield of rhizomes by 21%, plant height by 5% and weight of rhizome by 60% as compared to uninoculated controls [196]. Furthermore, a significant improvement in the curcumin content of P. fluorescens and B. megaterium inoculated turmeric plants has been reported [197]. Likewise, an increase in the number of leaves, height and biomass of shoot, dry biomass of rhizome and curcumin content of turmeric was recorded when plants were bioprimed with asymbiotic N₂ fixer A. chroococcum [194]. Kumar et al. [198] also observed an identical increase in biological properties, yield attributes and curcumin content of P. fluorescens bacterized turmeric plants while antioxidant activity, flavanoids, phenol content and curcumin content were significantly enhanced when the rhizome of turmeric plants was inoculated with a dual culture of PGPR and AM-fungi [199].

4.6. Piper nigrum

Black pepper (Piper nigrum L.) is a commercial spice crop grown in different regions of the world. Both endophytic and biosensor bacteria can increase the growth and quality of black pepper [200]. The application of beneficial bacteria B. tequilensis strain NRRLB-41771 revealed a significantly greater root morphogenesis in black pepper when grown under greenhouse conditions. Moreover, an increment in the total N and P content in soil and plant tissues was recorded in rhizobacteria inoculated black pepper plants [201]. In addition, Serratia nematodiphila expressing P solubilisation activity, IAA secretion ability and siderophore excretion potential significantly influenced the growth of P. nigrum grown under greenhouse using sand: soil: FYM [202].

4.7. Basil

The genus Ocimum is a member of family Lamiaceae that contains over 150 cultivated aromatic perennial herb species, spread all over tropical and temperate regions [203]. Ocimum sp. is rich in essential oils consisting of various phenolic compounds and other natural products including polyphenols such as flavonoids and anthocyanins [204,205]. The leaves and flowering parts of sweet basil (Ocimum basilicum) have antispasmodic, aromatic, carminative and digestive properties [206]. Additionally, in various food preparations, basil is used as an ingredient in its fresh form, especially in Mediterranean cuisine. The essential oil derived from basil possesses antimicrobial [207] and insecticidal [208] properties while, due to its pleasant aroma, basil oil is used in food industries, pharmaceuticals, cosmetics, aromatherapy, etc. Considering the medicinal importance of basil and to facilitate the safe production of basil, Mangmang et al. [209] revealed that the A. brasilense inoculation increased the length, height and dry biomass of basil by 90%, 19%, and 44%, respectively, while plants also had more developed (25%) and bigger (61%) leaves. The bacterial inoculation altered the metabolic activities of inoculated plants and augmented the peroxidase activity and total concentration of P. Basil plants previously inoculated with bacterial strains and grown under an aquaponics system showed superior growth and upsurge in the leaf area, fresh herbage yield and root weight by 27, 11 and 11%, respectively. The increment in peroxidase activity (73%), IAA (27%) and protein contents (20%) following inoculation suggests that the PGPR, similar to other plants, could also serve as an important microbiological tool to facilitate the growth and yield stability of basil. Likewise, the P. putida strain 41, A. chroococcum strain 5 and A. lipoferum strain OF enhanced various biological and chemical parameters of basil plants such as dry and fresh weight of roots and shoots, height of shoots and N, P, K content. The essential oils content was also greatly enhanced relative to uninoculated plants [210].

4.8. Rosemary

Rosemary (Rosmarinus officinalis L.) belongs to family Labiatae and grows generally as a shrub or sometimes as herbaceous plants [211]. Morphologically, rosemary is an aromatic plant with dark green leaves and is cultivated mainly in the Mediterranean region. The leaves and flowering tops of rosemary plants are a rich source of flavonoids and phenolic acids, especially the rosmarinic acid and an essential oil which contains compounds such as pinene, camphene, cineole, borneol and camphor, all of which exhibit medicinal and stimulatory effects [212,213]. The oils extracted from rosemary have numerous therapeutic and antiseptic applications [214]; in addition, they are used in making soaps, perfumes, cosmetics, etc. They are also used in flavouring and preservation of foods [215]. Like many other plants, rosemary is also responsive to inoculations and has displayed improved yield following PGPR application [216]. The biological properties such as number of lateral of branches (43.95–46.39%), height of stem (29.04–38.57%), length of roots (32.31–37.14%), shoots (34.76–40.91%) and dry biomass of roots (62.89–70.70%) increased significantly due to inoculation of B. subtilis, P. aeruginosa and Cedecia lapagei. Additionally, the physiological features such as total chlorophyll, phenol and carotenoids of R. officinalis were augmented up to 31, 25 and 40% by bacterial inoculation relative to control. Besides this, a considerable improvement in plant nutrients (NPK) was also recorded [217]. The application of PGPR also significantly increased the essential oils of rosemary plants even while growing in salinized conditions [218]. The commercial biofertilizers A. chroococcum and P. fluorescens, when applied with humic and fulvic acids, had variable but significant impact on the number of stem, growth of plants, oil extract volume and plant height [219]. Studies by Kasmaei et al. [220] also revealed an obvious increment in dry biomass, nutrient mobilization, photosynthetic factors, carbohydrate, flavonoid and essential oils of rosemary by organic fertilizers, especially with composite application of PGPB and compost or biochar, but proline content declined in all treatments. This finding validated the positive impacts of PGPR, compost and boichar of Azolla on rosemary production and consequently increased the nutrient uptake and protected chlorophyll from degradation and enhanced the foliage quality [220].

4.9. Hyssopus

Hyssop (Hyssopus officinalis), which belongs to the family Lamiaceae [221], is one of the most important medicinal plant that produces essential oils and phenolic compounds. Phytoconstituents of this plant include quercetin-7-O-β-d-apiofuranosyl-(1→2)-β-d-xylopyranoside and quercetin-7-O-β-Dapiofuranosyl-(1→2)-β-d-xylopyranoside-3′-O-β-d-glucopyranoside. It also possesses antioxidant, anticonvulsant, antifungal, antimicrobial, antihemolytic, antiulcer, antispasmodic and many other medicinal properties [222]. The research conducted so far has shown that combination of organic materials and biofertilizers can be effective in enhancing the morphological features and productivity of hyssop. As an example, the organic manures (compost/vermicompost) and biofertilizer (Azotobacter and Azospirillum) used together had impressive positive influence on the biological and yield attributes, herb dry yield and concentration and yield of essential oil in Hyssop grown under field conditions [223]. Additionally, when Hyssopus plants were bacterized with PGPR, the growth regulators, plant metabolites and vitamins, etc. were increased which directly and significantly influenced the growth and development of plants. Among various rhizobacteria applied, Azospirillum demonstrated greatest valuable impact on the growth of Hyssopus plants, leading eventually to a substantial increase in all biological traits [224]. The beneficial impact of some PGPR on the overall growth and development of some medicinal plants is summarized in

Table 2. Impact of plant growth promoting rhizobacteria on bio-chemical properties and yield of selected medicinal plants.

S. No.	Medicinal Plant	Bioinoculant Used	Bio-Chemical Traits	References
1.	Limonium sinense	Bacillus flexus KLBMP 4941	Enhanced synthesis of chlorophyll and flavonoids, osmotic regulation, increased activity of antioxidant enzymes and regulation of sodium/potassium homeostasis	[225]
2.	Papaver somniferum	Pseudomonas putida (WPTe)	Indole acetic acid production, growth and yield improvement, increased chlorophyll synthesis and stomatal conductance	[226]
3.	Mentha pulegium L.	Azotobacter chroococcum and Azospirillum brasilense	Mitigation of drought resistance, enhanced production of ascorbic acid, soluble sugars, proteins, flavonoids, total phenolics, oxygenated monoterpenes and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity	[227]
4.	Foeniculum vulgare sp. vulgare Mill	Bacillus polymyxa, A. chroococcum, and Azospirillum lipoferum	Increased plant attributes such as length, number of branches/plant, fresh weight of fruits and herbs, fruit yield, oil components, total phenolic content and photosynthesis	[228]
5.	Dracocephalum moldavica	Micrococcus yunnanensis and Claroideoglomus etunicatum	Enhanced photosynthesis, nutrient and dry matter accumulation, higher content of rosmarinic acid, eugenol, hesperetin and p-coumaric acid	[229]
6.	Panax ginseng	Rhizobium panacihumi	Biomass accumulation, higher proline levels, increased soluble sugar and total phenolic contents, scavenging response to oxidative stress, mitigation of aluminium stress	[230]
7.	Panax ginseng Meyer	Mesorhizobium panacihumi DCY119T	Production of siderophores, scavenging of ROS to circumvent iron stress, enhanced IAA production	[231]
8.	Anoectochilus formosanus (Wall.) Lindl. (YYB) and Anoectochilus roxburghii (Wall.) Lindl. (MRH)	Bacillus velezensis strains (ZJ-11 and D2WM)	Increased plant biological parameters such as height and weight, significant increase in flavonoid and kinsenoside content and reduction in the population of pathogenic fungi	[136]
9.	Mentha piperita	Bacillus amyloliquefaciens (GB03) and Pseudomonas fluorescens WCS417r	Bacterial inoculation improved various plant growth parameters including leaf number, surface area, and biomass, reduction in membrane lipid peroxidation and oxidative stress and increased phenolic content	[232]
10.	Mentha piperita	Pseudomonas fluorescens WCS417r, P. putida SJ04 and B. subtilis GB03	Rise in jasmonic acid (JA) and salicylic acid (SA) leading to glandular trichome density	[233]
11.	Mentha piperita	Pantoea agglomerans and P. putida	Phosphate solubilisation, enhanced biological attributes such as leaf number and length, number of stems, overall dry biomass accumulation and photosynthetic pigments	[234]
12.	Mentha arvensis	Bacillus flexus (Sd-30), Stenotrophomonas spp. (Az-30), and Brevibacterium halotolerans (Sd-6)	Enhanced photosynthesis, higher oil content and nutrient accumulation	[235]
13.	Stevia rebaudiana	PGPR strains (CA1001, CA2003 and CA2004)	IAA production	[236]
14.	Mentha piperita	P. fluorescens, P. putida, and B. subtilis	Enhanced emission of volatile organic compounds and phenolics	[237]
15.	Ocimum basilicum L.	Two biofertilizers containing Pseudomonas sp., Azospirillum sp., Bacillus sp., Azotobacter sp.	Increased dry matter accumulation in shoots and essential oils	[238]
16.	Hyptis Suaveolens	Bacillus pumilus and Pseudomonas Pseudoalcaligenes	Reduction of salinity impact, enhanced carotenoids and chlorophyll pigment	[239]
17.	Handroanthus Ochraceus	Azospirillum brasilense	Increased root volume, dry matter accumulation, enhanced density or size of glandular trichomes	[240]
18.	Codonopsis pilosula (Franch.) Nannf.	Bacillus amyloliquefaciens GB03	Two-fold increase in content of lobetyolin	[241]

.

5. Abiotic Stress Alleviation in Medicinal Plants by Beneficial Rhizobacteria

Similar to many other plants, medicinal plants are also susceptible to various abiotic stresses such as drought, salinity, heavy metals, flooding, cold, nutrient deficiency [242,243] and biotic stresses [244] that have detrimental impact on growth, development and secretion of phytoconstituents. Findings indicate challenges akin to physiological imbalances such as overproduction of stress hormone, ethylene and nutritional imbalances that may influence the growth and therapeutic features of medicinal plants [245]. Reduction in the chlorophyll fluorescence (Fv/Fm) and relative water content (RWC) are some other harmful impacts on medicinal plants (e.g., Mentha pulegium) growing under stress. The antioxidant enzyme activity, lipid peroxidation, production of secondary metabolites and DPPH radical scavenging activity, however, increases in such valuable plants to combat stress. Application of rhizospheres/phyllospheres/endophytes PGPR can be useful in promoting the growth and yield stability while protecting the plants from harmful effects of single or multiple stress factors. In this context, inoculation of PGPR, A. chroococcum and A. brasilense substantially circumvented the lethal effects of drought stress and simultaneously improved the concentration of phytochemicals such as abcissic acid (ABA), soluble sugars, phenolic and flavonoids, etc. in pennyroyal plants even under severe drought stress conditions [227]. The alleviation of drought stress in Glycyrrhiza uralensis, a plant of extreme medicinal importance, by B. pumilus is also reported [34]. In this case, the chlorophyll content, photosynthetic rate and water state were improved in B. pumilus primed plants relative to non-inoculated plants, wherein the chloroplast membrane system was ruptured under water deficit conditions. The growth and survival rate of pepper plants was also enhanced following inoculation of IAA and ACC deaminase positive B. licheniformis strain K11 under drought stress [246]. The uninoculated pepper plants, however, did not survive 15 days after exposure to drought conditions, but the inoculated plants, on the contrary, survived for longer duration even under stress. A similar increase in the chlorophyll content and mineral uptake in sweet basil plants bacterized with Pseudomonas sp., B. lentus and A. brasilense under drought conditions is described [247]. Additionally, the PGPR inoculated Hyoscyamus niger, an important herbal plant which has high quantity of tropane alkaloids, demonstrated positive results. Inoculation with P. putida and P. fluorescens abated the toxic effects caused due to water scarcity in henbane plants and concurrently improved the growth and the content of tropane alkaloids [248].

Apart from drought stress, the performance of medicinal plants is also destructively influenced by high salt levels [249,250], which are though can be circumvented by PGPR inhabiting rhizospheres, phyllospheres or endospheres [245,251,252]. Recently, coriander seeds inoculated with composite cultures of A. brasilense and A. chroococcum grown with variable concentrations of salts displayed a noteworthy enhancement in the chlorophyll a and b content, grain yield, stem biomass and total plant biomass. Moreover, the dual inoculation significantly enhanced the antioxidant enzymes, CAT, but decreased the concentration of APX and GPX compared to uninoculated plants. A substantial decrease in Na but a considerable increase in K concentration in coriander foliage was also recorded in co-inoculated plants. Together, this finding can be used to solve coriander production problems under high salinity situations [253]. The interactions between PGPR and medicinal plants under various abiotic stresses are summarized in Figure 3.

Bidgoli et al. [218] showed that the yield of essential oil of Rosmarinus officinalis plants was enhanced when the plants were inoculated with rhizobacterial strain P. fluorescens over uninoculated plants, even under salinity stress conditions. The increment in the growth and yield of Mentha arvensis plants was recorded following inoculation with Halomonas desiderata and Exiguobacterium oxidotolerans, even under varying levels (100, 300 and 500 mM) of salts [254]. Generally, PGPR strains significantly increased the yield which, however, varied considerably with variation in salt concentrations. For instance, H. desiderata produced maximum yield at 100 and 300 mM NaCl, whereas E. oxidotolerans showed maximum yield at 500 mM NaCl concentration. Likewise, salt stress alleviation in medicinal plant Sylebum marianum and pepper following inoculation with halotolerant IAA positive P. aureantiaca strain TSAU22 and P. extremorientalis strain TSAU20 under saline conditions is reported [255,256]. Similar to salinity and drought stress, medicinal plants also suffer from toxicity of heavy metals which, however, can be relieved by the application of PGPR [257]. As an example, the siderophore-producing metal tolerant Mesorhizobium panacihumi strain DCY119T provided IAA to the plants, thereby promoting seedling growth and thus, eventually, conferring metal resistance to medicinal plant Panax ginseng. It was further established that M. panacihumi strain DCY119T could be employed for bioremediation purposes to improve the growth of ginseng plants in metal polluted soils [231].

6. Conclusions and Future Prospects

The pharmacological applications of medicinal plants depend largely on their secondary metabolites and bioactive phytochemicals. Due to the remarkable ability to influence human health positively, medicinal plants have become one of the most powerful and effective natural remedies in medical science. However, the cultivation of medicinal plants is severely threatened by abiotic stresses and injudicious applications of agrochemicals. The PGPR in this regard provides solutions to the problems of inexpensive and environmentally hazardous fertilizers that can enhance the overall growth, yield and quality of phytoconstituents of medicinal plants. In addition, stress tolerant PGPR also alleviate the various abiotic challenges and, in doing so, they allow the medicinal plants to grow efficiently even under a perturbed environment. Application of such beneficial microbes will provide four obvious benefits—(i) savings/reduction in fertilizers and pesticides input, (ii) protection of soil quality and biodiversity, (iii) restoration of declining herbal cultivation area and (iv) availability of natural medicines at reasonably low cost to every section of society and, therefore, the dramatic improvement in the human health systems. Therefore, PGPR mediated growth improvement of medicinal plants and yield optimization of bioactive phytochemicals opens up some new avenues in the development of low-cost herbal medicines, which may help the public during health care systems failures.