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Optical Tweezers in Sensing Technologies

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Optical Sensors".

Deadline for manuscript submissions: closed (25 March 2026) | Viewed by 1392

Special Issue Editor


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Guest Editor
School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA
Interests: on-chip optical tweezers; optical manipulations in dry environments; metasurfaces; silicon photonics

Special Issue Information

Dear Colleagues,

Optical tweezers are a laser-based technique that uses light to trap and manipulate micro/nano-objects. Optical tweezers are developed as advanced tools for super-highly precise sensing, which can be used for the measurement of forces with subfemtonewton sensitivity, displacements with subnanometer sensitivity, and masses with attogram sensitivity, therefore enabling its wide applications in single molecules sensing, gravitational waves detection, and quantum behavior investigation of macroscopic mechanics. Miniaturized optical tweezers, including fiber optical tweezers, on-chip integrated tweezers, and metasurface tweezers, have been developed. These new types of optical tweezers are portable, autonomous, integrable, and able to interface with other existing technologies, including microfluidics and ion traps in a quantum chip, and show more advantages in sensing.

The aim of this Special Issue is to highlight the most recent research regarding optical tweezers in sensing technology. Potential topics include, but are not limited to, the following:

  • Optical tweezers;
  • Plasmonic tweezers;
  • Metalens tweezers;
  • On-chip actuation and sensing;
  • Microfluidics;
  • Single molecules detection;
  • Ultrasensitive sensing;
  • Quantum sensing.

Dr. Jinsheng Lu
Guest Editor

Manuscript Submission Information

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Published Papers (1 paper)

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Research

15 pages, 3599 KB  
Article
Real-Time Probing of Molecular Affinity Using Optical Tweezers
by Joana Teixeira, José A. Ribeiro, Marcus Monteiro, Nuno A. Silva and Pedro A. S. Jorge
Sensors 2026, 26(6), 1814; https://doi.org/10.3390/s26061814 - 13 Mar 2026
Viewed by 396
Abstract
The ability to assess molecular binding kinetics in real time is critical for advancing our understanding of molecular interactions in biochemical and biotechnological systems. This work presents a novel optical tweezer (OT)-based method to monitor molecular affinity in real time, focusing on the [...] Read more.
The ability to assess molecular binding kinetics in real time is critical for advancing our understanding of molecular interactions in biochemical and biotechnological systems. This work presents a novel optical tweezer (OT)-based method to monitor molecular affinity in real time, focusing on the high-affinity streptavidin–biotin system as a model. Transparent poly(methyl methacrylate) (PMMA) microparticles functionalized with streptavidin were trapped before, during, and after binding with biotinylated bovine serum albumin (biotin–BSA), enabling the analysis of forward-scattered signals to detect nanoscale changes in particle size. By applying the Power Spectral Density method, the friction coefficient of individual particles was calculated, allowing for real-time tracking of binding dynamics and the estimation of the association rate constant (kon106M1s1). These results are consistent with literature values and demonstrate the potential of this OT-based approach for non-invasive, label-free detection of molecular interactions. Compared to existing techniques, such as atomic force microscopy and cantilever-based sensors, this method offers significant advantages, including real-time monitoring, adaptability to different bioaffinity systems, and compatibility with miniaturized setups. This work establishes a foundation for using OT-based tools to monitor high-affinity molecular interactions in real time. While demonstrated here using biotinylated BSA as a model ligand, future studies will explore the method’s applicability to smaller ligands and more subtle surface modifications. Full article
(This article belongs to the Special Issue Optical Tweezers in Sensing Technologies)
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