Special Issue "Fluorescence Based Biosensing Applications"
Deadline for manuscript submissions: 31 January 2021.
Interests: Fluorescence; Luminescence; Luciferase; Protein immobilization; Protein/peptide probes; Inorganic surface; Enzymatic sensors; Point-of-care testing; On-site detection; Microscopic analysis; Extracellular vesicles; Exosome
Fluorescence-based biosensing has been applied in various fields such as medical diagnostics, environmental monitoring, and inspection for food safety. Fluorescence detection is based on the use of fluorophores that emit light when excited by light of a shorter wavelength. In the simplest enzymatic fluorescence-based biosensing, an increase in the fluorescence intensity, resulting from the enzymatic conversion of a fluorogenic substrate to a fluorophore, is measured. Nowadays, numerous parameters besides the intensity, such as fluorescence anisotropy, decay time, energy transfer, and quenching have been explored in fluorescence-based biosensing. Aggregation-induced emission is a novel photophysical phenomenon which offers a new platform of fluorescence application. These fluorescence signals can also be used to monitor dynamic intracellular events of protein conformational changes upon protein-protein or protein-target interaction. Thus, fluorescence-based biosensing can be exploited to develop high-throughput screening for drug discovery. On the other hand, fluorescent labeling of a specific binder (e.g., antibodies or proteins) to a target allows the use of fluorescent microscopy, which has numerous advantages compared to other kinds of optical and even electron microscopy. The development of fluorescent specific probes could expand the possibilities of biosensing capabilities. This Special Issue aims to present the wide range of exciting applications using the latest technologies and methodologies developed in fluorescence-based biosensing.
Prof. Akio Kuroda
Manuscript Submission Information
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- Fluorescence-based biosensing
- Medical diagnostics
- Environmental monitoring
- Inspection for food safety
- Drug discovery
- Fluorescence anisotropy
- Fluorescence energy transfer
- Aggregation-induced emission
- Fluorescent microscopy