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Plants
Special Issues
Plant Tissue Culture and Plant Regeneration—2nd Edition
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Plant Tissue Culture and Plant Regeneration—2nd Edition

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Development and Morphogenesis".

Deadline for manuscript submissions: 30 May 2026 | Viewed by 907

Special Issue Editors

Dr. Douglas A. Steinmacher

E-Mail Website
Guest Editor
Vivetech Agrociências, Marechal Cândido Rondon 85960-000, Brazil
Interests: plant tissue culture
Special Issues, Collections and Topics in MDPI journals
Dr. Taras Pasternak

E-Mail Website
Guest Editor
Instituto de Bioingeniería, Universidad Miguel Hernández, 03202 Elche, Spain
Interests: physiology and cell biology; plant tissue culture; molecular biology; cytochemistry; microscopy
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Plant tissue culture and plant regeneration constitute crucial facets of plant biotechnology, spanning several scientific and industrial domains. In recent years, we have witnessed significant advancements in understanding the underlying mechanisms controlling in vitro plant regeneration, accompanied by the rapid evolution of specialized equipment and strategies for enhancing this process. This development has garnered the attention of both plant scientists and industries. Nonetheless, an ongoing demand exists to develop further strategies to aid the selection and regeneration of superior genotypes, thereby refining protocols for enhanced reproducibility. As such, the creation of a meticulous fine-tuning process is imperative. Considering these developments, we are announcing a Special Issue covering all aspects of plant tissue culture and biotechnological processes, including the use of algorithms to improve protocols, aspects of somatic embryogenesis and organogenesis, cryopreservation, the use of temporary immersion bioreactors, and new gene-editing technology to enhance shoot proliferation.

This Special Issue will accept the submission of original research articles, short communications, and comprehensive reviews.

We look forward to receiving your contributions.

Dr. Douglas A. Steinmacher
Dr. Taras Pasternak
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plant biotechnology
  • micropropagation
  • organogenesis
  • somatic embryogenesis
  • temporary immersion system
  • data-driven model
  • shoot proliferation
  • gene editing

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Related Special Issue

Published Papers (2 papers)

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Research

16 pages, 801 KiB  
Article
Superior In Vitro Responses of a Native Rose Genotype to Driver Kuniyuki Walnut (DKW) Medium in a Comparative Study Using Natural and Synthetic Plant Growth Regulators
by Mahboubeh Davoudi Pahnekolayi, Zahra Parchianloo, Majid Babouyehdarabi and Meysam Ghasemi
Plants 2025, 14(16), 2606; https://doi.org/10.3390/plants14162606 - 21 Aug 2025
Abstract
Rosa canina is one of the precious native rose rootstocks with a high reputation among plant producers, which has potential horticultural and pharmacological properties related to the cosmetic values and the production of secondary metabolites. Due to high horticultural consumption, applying the plant [...] Read more.
Rosa canina is one of the precious native rose rootstocks with a high reputation among plant producers, which has potential horticultural and pharmacological properties related to the cosmetic values and the production of secondary metabolites. Due to high horticultural consumption, applying the plant tissue culture technique as a major tool for healthy and massive-scale production of R. canina plants is not unexpected. However, the response of R. canina in vitro plantlets to various plant tissue culture ingredients is not well understood to tender an efficient applied protocol for qualitative and quantitative in vitro propagation. In this regard, the main objective of this study is to investigate the influence of several abiotic in vitro variants including six plant tissue culture media formulations (McCown’s Woody Plant Medium (WPM), Murashige and Skoog (MS), Van der Salm (VS), Schenk and Hildebrant (SH), Driver Kuniyuki Walnut (DKW), and Gamburg B5 (B5)) in combination with four concentrations (0, 1.5, 3, 4 mgL−1) of two types of cytokinins (6-Benzyaminopurine (BAP) and Kinetin (Kin)) simultaneously. Notably, it is perceived that DKW culture medium containing 1.5 mgL−1 BAP and 0.1 mgL−1 NAA is the best treatment for both in vitro morphological and flowering properties. Full article
(This article belongs to the Special Issue Plant Tissue Culture and Plant Regeneration—2nd Edition)
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18 pages, 1967 KiB  
Article
Optimizing Growth Regulator Concentrations for Cannabis sativa L. Micropropagation
by Gabrielle A. Johnson, Carissa L. Jackson, Antonio Timoteo, Jr., Papaiah Sardaru, Michael H. Foland, Purushothaman Natarajan and Sadanand A. Dhekney
Plants 2025, 14(16), 2586; https://doi.org/10.3390/plants14162586 - 20 Aug 2025
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Abstract
In this study, the effect of growth regulators on shoot proliferation and rooting were evaluated to develop an efficient micropropagation protocol for the Cannabis sativa L. cultivars ‘Cherry Soda’ and ‘Purple’. Apical meristems were isolated from actively growing shoots of stock plants and [...] Read more.
In this study, the effect of growth regulators on shoot proliferation and rooting were evaluated to develop an efficient micropropagation protocol for the Cannabis sativa L. cultivars ‘Cherry Soda’ and ‘Purple’. Apical meristems were isolated from actively growing shoots of stock plants and transferred to Driver and Kuniyuki Walnut (DKW) culture medium containing either 0.0, 0.5, 1.0, 2.0, or 5.0 μM meta-Topolin to study their shoot proliferation response. Resulting shoot cultures were transferred to medium containing varying levels of Indole Acetic Acid (IAA), Indole Butyric Acid (IBA), or Naphthalene Acetic Acid (NAA), solely or in combination, and were subjected to a 10-day dark incubation followed by a 16 h/8 h light/dark period to identify the best treatment for root production. Among the different shoot proliferation treatments studied, the maximum number of shoots was produced on the control medium that was devoid of any meta-Topolin. Cultures grown on medium containing 5.0 μM meta-Topolin exhibited hyperhydricity, where shoots appeared translucent and pale green in color; were characterized by water-soaked lesions; and leaves appeared curled and brittle in contrast to healthy looking cultures. Among the various rooting treatments studied, shoots grown in the dark for 10 days exhibited the highest frequency of rooting on medium containing 4.0 μM NAA or 6.0 μM IBA + 1.0 μM NAA. Full developed plants with a robust shoot and root system were transferred to soil, acclimatized under conditions for high humidity, and then transferred to ambient conditions in 4 weeks. The micropropagation protocol developed here allows for rapid multiplication of disease-free plants in C. sativa cultivars. Full article
(This article belongs to the Special Issue Plant Tissue Culture and Plant Regeneration—2nd Edition)
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