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Research on Plant Virus Resistance with Peptides and Small RNA...
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Research on Plant Virus Resistance with Peptides and Small RNA Interference

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Protection and Biotic Interactions".

Deadline for manuscript submissions: closed (30 June 2021) | Viewed by 2996

Special Issue Editor

Dr. Séverine Lacombe

E-Mail Website
Guest Editor
Interactions Plantes Microorganismes et Environnement (IPME), IRD, CIRAD, Université Montpellier, 911 Avenue Agropolis BP64501, CEDEX 5, 34394 Montpellier, France
Interests: root-knot nematodes; plant viruses; RNA silencing suppressor proteins in plant–microorganism–vector interactions; small RNA mediated virus resistance; rice viruses; legume nanoviruses

Special Issue Information

Dear Colleagues, 

Plant viruses can attack many plants varieties all around the world. When these viruses target plants of agronomic interest, it can cause significant economic loss. Plants can deploy genetic resistances if they are available; however, viruses rapidly overcome them. In several cases, the only efficient and effective treatments are expensive and non-environmentally friendly chemicals. Therefore, new, efficient, environmentally friendly, and durable strategies are fundamentally required to fight against these rapidly evolving pathogens. The discovery of RNA silencing in the 1990s revolutionized our knowledge of plant–virus interactions. Its exploitation as a resistance strategy against plant viruses gave rise to significant hopes of creating powerful viral resistance reducing the need for chemical treatments. It is crucial that efforts continue in this way in order to deploy efficient and durable genetic resistance into plants of agronomic interest. This plays a vital role in maintaining global food safety. 

In this Special Issue titled "Research on Plant Virus Resistance with Peptides and Small RNA Interference", both fundamental and applied articles describing original and efficient viral resistance strategies in all types of plant backgrounds are welcome.

Dr. Séverine Lacombe
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plant virus resistance
  • peptides
  • small RNA
  • RNA silencing suppressor proteins
  • silencing suppressors
  • virulence effectors
  • plant defense
  • environmentally friendly

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Published Papers (1 paper)

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Research

17 pages, 1919 KiB  
Article
Optimized RNA-Silencing Strategies for Rice Ragged Stunt Virus Resistance in Rice
by Severine Lacombe, Martine Bangratz, Hoang Anh Ta, Thanh Duc Nguyen, Pascal Gantet and Christophe Brugidou
Plants 2021, 10(10), 2008; https://doi.org/10.3390/plants10102008 - 24 Sep 2021
Cited by 6 | Viewed by 2494
Abstract
Rice ragged stunt virus (RRSV) is one of the most damaging viruses of the rice culture area in south and far-eastern Asia. To date, no genetic resistance has been identified and only expensive and non-environmentally friendly chemical treatments are deployed to fight this [...] Read more.
Rice ragged stunt virus (RRSV) is one of the most damaging viruses of the rice culture area in south and far-eastern Asia. To date, no genetic resistance has been identified and only expensive and non-environmentally friendly chemical treatments are deployed to fight this important disease. Non-chemical approaches based on RNA-silencing have been developed as resistance strategies against viruses. Here, we optimized classical miRNA and siRNA-based strategies to obtain efficient and durable resistance to RRSV. miRNA-based strategies are involved in producing artificial miRNA (amiR) targeting viral genomes in plants. Classically, only one amiR is produced from a single construct. We demonstrated for the first time that two amiRs targeting conserved regions of RRSV genomes could be transgenically produced in Nicotiana benthamiana and in rice for a single precursor. Transgenic rice plants producing either one or two amiR were produced. Despite efficient amiR accumulations, miRNA-based strategies with single or double amiRs failed to achieve efficient RRSV resistance in transformed rice plants. This suggests that this strategy may not be adapted to RRSV, which could rapidly evolve to counteract them. Another RNA-silencing-based method for viral resistance concerns producing several viral siRNAs targeting a viral fragment. These viral siRNAs are produced from an inverted repeat construct carrying the targeted viral fragment. Here, we optimized the inverted repeat construct using a chimeric fragment carrying conserved sequences of three different RRSV genes instead of one. Of the three selected homozygous transgenic plants, one failed to accumulate the expected siRNA. The two other ones accumulated siRNAs from either one or three fragments. A strong reduction of RRSV symptoms was observed only in transgenic plants expressing siRNAs. We consequently demonstrated, for the first time, an efficient and environmentally friendly resistance to RRSV in rice based on the siRNA-mediated strategy. Full article
(This article belongs to the Special Issue Research on Plant Virus Resistance with Peptides and Small RNA Interference)
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