Innovation and Advanced Technology for Orchid Research

A special issue of Plants (ISSN 2223-7747).

Deadline for manuscript submissions: closed (10 March 2023) | Viewed by 3934

Special Issue Editors


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Guest Editor
Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Interests: orchid resources; genome evolution; environmental adaptation

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Guest Editor
Institute of environmental horticulture, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
Interests: orchid breeding; functional gene; floral development; tissue culture

Special Issue Information

Dear Colleagues,

Orchidaceae is one of the largest and most evolved families of monocot plants. Over the past 2000 years, more than 70,000 orchids have been cultivated as ornamental and medicinal plants, as well as food flavoring additives.

The present Special Issue encompasses the themes of this conference in the following research areas: orchid germplasm resources and ecological evolution; molecular biology of orchids; orchid breeding technology; tissue culture and rapid propagation technology; comprehensive control of diseases and pests; protected cultivation of orchids and intelligent agriculture; and orchid culture and science popularization. Please note that this Special Issue is also seeking original works that address this range of topics.

Dr. Zhongjian Liu
Dr. Fengxi Yang
Guest Editors

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Published Papers (1 paper)

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Research

13 pages, 1952 KiB  
Article
PhCHS5 and PhF3′5′H Genes Over-Expression in Petunia (Petunia hybrida) and Phalaenopsis (Phalaenopsis aphrodite) Regulate Flower Color and Branch Number
by Yuxia Lou, Qiyu Zhang, Qingyu Xu, Xinyu Yu, Wenxin Wang, Ruonan Gai and Feng Ming
Plants 2023, 12(11), 2204; https://doi.org/10.3390/plants12112204 - 2 Jun 2023
Cited by 6 | Viewed by 3257
Abstract
Flower breeders are continually refining their methods for producing high-quality flowers. Phalaenopsis species are considered the most important commercially grown orchids. Advances in genetic engineering technology have provided researchers with new tools that can be used along with traditional breeding methods to enhance [...] Read more.
Flower breeders are continually refining their methods for producing high-quality flowers. Phalaenopsis species are considered the most important commercially grown orchids. Advances in genetic engineering technology have provided researchers with new tools that can be used along with traditional breeding methods to enhance floral traits and quality. However, the application of molecular techniques for the breeding of new Phalaenopsis species has been relatively rare. In this study, we constructed recombinant plasmids carrying flower color-related genes, Phalaenopsis Chalcone synthase (PhCHS5) and/or Flavonoid 3′,5′-hydroxylase (PhF3′5′H). These genes were transformed into both Petunia and Phalaenopsis plants using a gene gun or an Agrobacterium tumefaciens-based method. Compared with WT, 35S::PhCHS5 and 35S::PhF3′5′H both had deeper color and higher anthocyanin content in Petunia plants. Additionally, a phenotypic comparison with wild-type controls indicated the PhCHS5 or PhF3′5′H-transgenic Phalaenopsis produced more branches, petals, and labial petals. Moreover, PhCHS5 or PhF3′5′H-transgenic Phalaenopsis both showed deepened lip color, compared with the control. However, the intensity of the coloration of the Phalaenopsis lips decreased when protocorms were co-transformed with both PhCHS5 and PhF3′5′H. The results of this study confirm that PhCHS5 and PhF3′5′H affect flower color in Phalaenopsis and may be relevant for the breeding of new orchid varieties with desirable flowering traits. Full article
(This article belongs to the Special Issue Innovation and Advanced Technology for Orchid Research)
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