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Plants
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Plant Tissue Culture and Biotechnology: Bridging Fundamental Research and Global...
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Plant Tissue Culture and Biotechnology: Bridging Fundamental Research and Global Challenges

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: 31 May 2026 | Viewed by 2152

Special Issue Editors

Prof. Dr. Zhongxiong Lai

E-Mail Website
Guest Editor
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Interests: horticultural crops; genomics; tissue culture; molecular biology; germplasm; somatic embryogenesis
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Yuling Lin

E-Mail Website
Guest Editor
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Interests: genomics and biotechnology; plant tissue culture; non-coding RNA; molecular biology; somatic embryogenesis
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Yuqiong Guo

E-Mail Website
Guest Editor
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Interests: tea processing; tea biotechnology; tea biochemistry; genomics; molecular biology
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Xu XuHan

E-Mail Website
Guest Editor
Institut de la Recherche Interdisciplinaire de Toulouse (IRIT-ARI), 31300 Toulouse, France
Interests: embryology; in vitro regeneration; molecular cell biology; environment science; organic ecology

Special Issue Information

Dear Colleagues,

Plant tissue culture has long been a cornerstone of plant biotechnology, which offers powerful tools for propagation, genetic improvement, conservation, and phytochemical production. Recent developments in molecular biology, omics technologies, and genome editing have further expanded the potential of in vitro culture systems, enabling unprecedented precision and efficiency in plant research and the industrial applications of plants.

This Special Issue aims to bring together recent advances and innovative approaches in plant tissue culturing that cover a wide range of species and applications. We invite original research articles and comprehensive reviews on topics including, but not limited to, the following:

  • In vitro propagation and micropropagation of economically or ecologically important plants;
  • Somatic embryogenesis, organogenesis, and callus cultures;
  • Plant regeneration systems for genetic transformation and genome editing;
  • Secondary metabolite production in plant cell and tissue cultures;
  • Protoplast culture, fusion, and synthetic seed technology;
  • Tissue culture-based conservation of rare or endangered species;
  • Mechanisms of in vitro morphogenesis within physiology, biochemistry, molecular biology, genomics, etc.;
  • Challenges and solutions for scaling up tissue culture for commercial production.

This Special Issue seeks to serve as a platform on which researchers can showcase both the fundamental and applied aspects of plant tissue culture, contributing to sustainable agriculture, conservation biology, and bioindustry.

We warmly invite you to contribute your latest findings to this exciting Special Issue.

Prof. Dr. Zhongxiong Lai
Prof. Dr. Yuling Lin
Prof. Dr. Yuqiong Guo
Prof. Dr. Xu XuHan
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plant cell culture
  • plant tissue culture
  • transcriptomics
  • genome/gene editing
  • cryopreservation
  • CRISPR
  • somatic embryogenesis

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (2 papers)

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Research

25 pages, 4633 KB  
Article
Optimization of Micropropagation and Metabolomic Analysis Under Different Light Qualities in Mussaenda pubescens Ait.f
by Li Sun, Jiajia Wu, Zilu Yang, Roudi Cai, Xiaoping Xu, Jiahui Li, Ning Tong, Muhammad Awais, Yuling Lin and Zhongxiong Lai
Plants 2025, 14(21), 3268; https://doi.org/10.3390/plants14213268 - 26 Oct 2025
Viewed by 574
Abstract
The current investigation utilized stem nodes from pre-established aseptic lines of Mussaenda pubescens as explants to optimize an efficient in vitro propagation protocol and investigated the effect of different light qualities (white, red, blue, and green) on metabolite accumulation in micropropagated plantlets. The [...] Read more.
The current investigation utilized stem nodes from pre-established aseptic lines of Mussaenda pubescens as explants to optimize an efficient in vitro propagation protocol and investigated the effect of different light qualities (white, red, blue, and green) on metabolite accumulation in micropropagated plantlets. The findings demonstrated that the optimal medium for shoot proliferation was Murashige and Skoog basal medium supplemented with 6-Benzylaminopurine 2.0 mg·L−1 and α-naphthaleneacetic acid 0.2 mg·L−1, achieving a multiplication coefficient of 12.2 after 30 days. Rooting was more effective on Murashige and Skoog basal medium containing α-naphthaleneacetic acid 0.1 mg·L−1 and activated charcoal 1 g·L−1, resulting in a 100% rooting rate. During acclimatization, a substrate mixture of perlite:vermiculite: peat soil (1:1:1) promoted vigorous root development with a 100% survival rate at post-transplantation. Light quality significantly influenced plant morphology: red light stimulated stem elongation, while blue light increased biomass accumulation. Broad-target metabolomics revealed distinct metabolite profiles under different light spectra, with differentially accumulated metabolites primarily belonging to terpenoids, organic acids, lipids, and flavonoids. Specifically, red light enhanced the levels of terpenoids and lipids; blue light promoted the synthesis of specific triterpenoid saponins and lipids; while green light increased the content of certain terpenes and broadly upregulated a wide spectrum of lipids. This work provides a robust framework for the commercial micropropagation of Mussaenda pubescens and elucidates the strategic use of light quality to enhance the production of its valuable medicinal metabolites, including terpenoids and lipids. Full article
(This article belongs to the Special Issue Plant Tissue Culture and Biotechnology: Bridging Fundamental Research and Global Challenges)
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17 pages, 1473 KB  
Article
Cascade Oxygen Control Enhances Growth of Nicotiana benthamiana Cell Cultures in Stirred-Tank Bioreactors
by Fuensanta Verdú-Navarro, Juan Antonio Moreno-Cid, Julia Weiss and Marcos Egea-Cortines
Plants 2025, 14(18), 2879; https://doi.org/10.3390/plants14182879 - 16 Sep 2025
Viewed by 959
Abstract
Plant cell cultures offer a promising platform for producing valuable biomolecules, yet their use in bioreactors remains under-optimized. Compared to animal or microbial cells, plant cells grow more slowly, limiting productivity. A common bioprocess strategy to improve yields involves controlling dissolved oxygen (DO) [...] Read more.
Plant cell cultures offer a promising platform for producing valuable biomolecules, yet their use in bioreactors remains under-optimized. Compared to animal or microbial cells, plant cells grow more slowly, limiting productivity. A common bioprocess strategy to improve yields involves controlling dissolved oxygen (DO) levels. However, little research has focused on combining agitation and aeration to regulate oxygen in plant cell cultures within bioreactors. The aim of this study was to evaluate the impact of an oxygen cascade mixing agitation and aeration on plant cell growth in stirred-tank systems. By maintaining 30% DO through this approach, the specific growth rate (µ) increased from 0.082 d−1 to 0.144 d−1 on average in Nicotiana benthamiana cultures at the 2 L scale, decreasing batch lengths from 21 to 10 days. These conditions were successfully replicated in a 7 L stainless-steel pilot bioreactor using previous values of geometry, kLa and NRE as scale-up criteria, obtaining a µ of 0.161 d−1. These results demonstrate that plant cell cultures’ efficiency can be enhanced by using standard bioprocess parameters. While this work confirms the feasibility of cascade oxygen control for improvements in growth, further studies are needed to evaluate its specific impact on biomolecule production across different systems. Full article
(This article belongs to the Special Issue Plant Tissue Culture and Biotechnology: Bridging Fundamental Research and Global Challenges)
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