Circadian Regulation of Growth, Development and Metabolism in Plants/Microorganisms

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Development and Morphogenesis".

Deadline for manuscript submissions: 31 January 2026 | Viewed by 951

Special Issue Editors


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Guest Editor
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, China
Interests: circadian regulation; growth and development; plants/microorganism
College of Horticulture, Yangling Sub-Center of National Center for Apple Improvement, Northwest A&F University, Xianyang, China
Interests: plants; metabolism; genome; morphological development

Special Issue Information

Dear Colleagues,

The circadian clock, which controls endogenous rhythms over approximately 24 hr, has evolved over 3.5 billion years to reach its present form in microorganisms (cyanobacteria). Three hundred years ago, De Mairan (1729) published the first scientific report on chronobiology and distinguished between the fundamental mechanisms of biological clocks and endogenous output rhythms (sleep movement) in plants (Mimosa pudica) under entrained and constant (free running) conditions. Expanding on the classical and long-term systemic research studies of Aschoff and Pittendrigh, knowledge of the canonical properties of circadian clocks (their free-running period, entrainment, and temperature compensation) has been gathered. However, our present understanding of the “circadian regulation mechanism” in plants/microorganisms is incomplete. In this Special Issue, studies should investigate input pathways, circadian oscillators, and output rhythms to further understand the regulation mechanism of growth, development, and metabolism (especially active secondary metabolites) in plant/microorganism circadian systems. In addition, plants and crops have inevitably faced and adapted to drastic changes in their environments owing to climate change. Articles are encouraged to discuss a wide range of issues concerning the circadian regulation of the physiological and biochemical processes of experimental systems in abiotic stresses and applications of “Chronbiotics” (e.g., melatonin and light) in biotechnology and agriculture through original experimental papers, opinions, and reviews.

Dr. Kamran Shah
Dr. Xiya Zuo
Guest Editors

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Keywords

  • circadian clock
  • regulation mechanism
  • growth and development
  • abiotic stresses
  • chronbiotics

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Published Papers (1 paper)

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Research

16 pages, 5032 KiB  
Article
Genome-Wide Analysis of the AT-Hook Gene Family in Malus sieversii and Functional Characterization of MsAHL13
by Da Zhang, Chao Zhao, Xin Liu, Han Wang, Bowei Zhu, Guodong Zhao, Dongmei Chen, Tongsheng Zhao, Haijiao Xu, Yingjie Wang, Chaohong Zhang and Xinsheng Zhang
Plants 2025, 14(17), 2625; https://doi.org/10.3390/plants14172625 (registering DOI) - 23 Aug 2025
Abstract
AT-hook motif nuclear-localized (AHL) proteins are pivotal in plant growth, development, and stress responses. Nevertheless, there is limited research on AHL proteins in Malus sieversii. Our study identified 25 AHL genes from the M. sieversii genome, named MsAHL1–MsAHL25. The encoded protein sequences [...] Read more.
AT-hook motif nuclear-localized (AHL) proteins are pivotal in plant growth, development, and stress responses. Nevertheless, there is limited research on AHL proteins in Malus sieversii. Our study identified 25 AHL genes from the M. sieversii genome, named MsAHL1–MsAHL25. The encoded protein sequences had lengths ranging from 195 to 554 amino acids, molecular weights from 19.17 to 58.53 kDa, and isoelectric points from 4.67 to 10.09. Chromosomal mapping revealed that these 25 genes were unevenly distributed across 10 chromosomes. Collinearity analysis of AHL genes in M. sieversii implied that gene loss might have occurred during its evolution. The phylogenetic tree classified the AHL proteins of M. sieversii into two subfamilies, showing a close relationship with multiple proteins of M. domestica. Promoter analysis indicated that the AHL genes in M. sieversii harbored numerous stress- and hormone-responsive elements, suggesting their potential role in various stress responses. qRT-PCR analysis of six representative MsAHLs under biotic and abiotic stresses demonstrated that the expression of MsAHL13, MsAHL15, and MsAHL17 was significantly upregulated under salt, drought, and cold stresses, while MsAHL01 expression was inhibited under low-temperature stress. All six MsAHLs were induced by the pathogen Valsa mali. Subcellular localization analysis of the specifically expressed protein MsAHL13 showed its nuclear location. Furthermore, luciferase and yeast two-hybrid assays confirmed the in vitro physical interaction between the MsAHL13 and MsMYB1 proteins. This research offers an important theoretical basis for further exploration of the functional mechanisms of this gene family in responding to environmental stresses. Full article
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