Advanced Optical Microscopy and Imaging Technology

A special issue of Photonics (ISSN 2304-6732).

Deadline for manuscript submissions: closed (15 November 2024) | Viewed by 6517

Special Issue Editors


E-Mail Website
Guest Editor
School of Medicine, Yale University, 333 Cedar Street, New Haven, CT 06510, USA
Interests: super-resolution microscopy; isoSTED; femtosecond lasers; nonlinear optical microscopy; label-free fluorescence microscopy; cartilage; second harmonic generation microscopy; spectroscopy; biophotonics
CLEMSON-MUSC Bioengineering Program, Clemson University, 68 President Street, Charleston, SC 29425, USA
Interests: deep tissue imaging; three-dimensional imaging of tissue functions; confocal microscopy; nonlinear optical microscopy; STED microscopy; label-free imaging; aberration correction; deep/machine learning; clinical imaging; osteoarthritis

Special Issue Information

Dear Colleagues,

The area of optical microscopy and imaging technology is ever-evolving to extend the ability of researchers to elucidate intricate biological structures and functions. This is a key area in biology, biophysics, and medicine. There are continued and unmet needs for deeper, faster, and higher-resolution imaging across a multitude of biological specimens. Technology advances in photonics, optical systems, fluorescence labeling methods, and sample preparations continue to drive many important discoveries.

This Special Issue, entitled “Advanced Optical Microscopy and Imaging Technology”, aims to compile significant research and review articles on a wide range of optical microscopy technologies and imaging applications that reflect the current development and future directions of the field. We are pleased to invite you to submit your manuscripts to this Special Issue. Topics include, but are not limited to, the following:

  • Advances in super-resolution microscopy: STED, single-molecule localization microscopy, structured illumination microscopy, MINSTED, MINFLUX, etc.;
  • Developments in nonlinear optical microscopy: two-photon, three-photon fluorescence microscopy, second harmonic and third harmonic generation microscopy, stimulated Raman scattering microscopy;
  • Technological advances and applications of light-sheet microscopy;
  • Novel fluorescence labeling and sample preparation methods or applications: e.g.,: expansion microscopy, pan-expansion microscopy, exchangeable fluorescent labeling;
  • Applications of adaptive optics in optical microscopy aberration correction;
  • Progress and developments in confocal microscopy, computational imaging, and phase contrast imaging;
  • Deep learning-aided microscopy implementation technology, for example, super-resolution microscopy, denoise, and aberration correction technology;
  • Developments in multimodal optical correlative imaging.

We look forward to receiving your contributions.

Dr. Yang Li
Dr. Tong Ye
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Photonics is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • super-resolution microscopy
  • expansion microscopy
  • exchangeable fluorescent labeling
  • light-sheet microscopy
  • computational imaging
  • structural illumination microscopy
  • nonlinear optical microscopy
  • deep learning

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Published Papers (3 papers)

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Research

11 pages, 5796 KiB  
Article
Real-Time Resolution Enhancement of Confocal Laser Scanning Microscopy via Deep Learning
by Zhiying Cui, Yi Xing, Yunbo Chen, Xiu Zheng, Wenjie Liu, Cuifang Kuang and Youhua Chen
Photonics 2024, 11(10), 983; https://doi.org/10.3390/photonics11100983 - 19 Oct 2024
Cited by 1 | Viewed by 1052
Abstract
Confocal laser scanning microscopy is one of the most widely used tools for high-resolution imaging of biological cells. However, the imaging resolution of conventional confocal technology is limited by diffraction, and more complex optical principles and expensive optical-mechanical structures are usually required to [...] Read more.
Confocal laser scanning microscopy is one of the most widely used tools for high-resolution imaging of biological cells. However, the imaging resolution of conventional confocal technology is limited by diffraction, and more complex optical principles and expensive optical-mechanical structures are usually required to improve the resolution. This study proposed a deep residual neural network algorithm that can effectively improve the imaging resolution of the confocal microscopy in real time. The reliability and real-time performance of the algorithm were verified through imaging experiments on different biological structures, and an imaging resolution of less than 120 nm was achieved in a more cost-effective manner. This study contributes to the real-time improvement of the imaging resolution of confocal microscopy and expands the application scenarios of confocal microscopy in biological imaging. Full article
(This article belongs to the Special Issue Advanced Optical Microscopy and Imaging Technology)
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12 pages, 4657 KiB  
Article
In Situ Structural Characterization of Cardiomyocyte Microenvironment by Multimodal STED Microscopy
by Zhao Zhang, Bruce Z. Gao and Tong Ye
Photonics 2024, 11(6), 533; https://doi.org/10.3390/photonics11060533 - 3 Jun 2024
Viewed by 3411
Abstract
Within the myocardium, cardiomyocytes reside in a complex and dynamic extracellular matrix (ECM) consisting of a basement membrane (BM) and interstitial matrix. The interactions between cardiomyocytes and the myocardial ECM play a critical role in maintaining cardiac geometry and function throughout cardiac development [...] Read more.
Within the myocardium, cardiomyocytes reside in a complex and dynamic extracellular matrix (ECM) consisting of a basement membrane (BM) and interstitial matrix. The interactions between cardiomyocytes and the myocardial ECM play a critical role in maintaining cardiac geometry and function throughout cardiac development and in adult hearts. Understanding how the structural changes of the myocardial ECM affect cardiomyocyte function requires knowledge of pericellular structures. These structures are of a size beyond the resolution of conventional optical microscopy. Here, we demonstrated multi-scale and multi-aspect characterization of the cardiomyocyte microenvironment in myocardial tissue sections using multimodal stimulated emission depletion (STED) microscopy. Second harmonic generation and autofluorescence facilitated multiplexed imaging, enabling the interpretation of protein distribution in 3D. STED imaging modality revealed BM structures of cardiomyocytes and myocardial capillaries at the subdiffractional level. Moreover, meaningful measurements retrieved from acquired images, such as sarcomere length and capillary density, enabled quantitative assessment of myocardial structures. Full article
(This article belongs to the Special Issue Advanced Optical Microscopy and Imaging Technology)
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18 pages, 2242 KiB  
Article
Role of Phase Information Propagation in the Realisation of Super-Resolution Based on Speckle Interferometry
by Yasuhiko Arai
Photonics 2023, 10(12), 1306; https://doi.org/10.3390/photonics10121306 - 26 Nov 2023
Cited by 2 | Viewed by 1015
Abstract
Super-resolution technology is important not only in bio-related fields but also in nanotechnology, particularly in the semiconductor industry, where fine patterning is required and super-resolution is essential. However, observing microstructures beyond the diffraction limit proposed by Abbe and Rayleigh is considered impossible because [...] Read more.
Super-resolution technology is important not only in bio-related fields but also in nanotechnology, particularly in the semiconductor industry, where fine patterning is required and super-resolution is essential. However, observing microstructures beyond the diffraction limit proposed by Abbe and Rayleigh is considered impossible because of diffraction in traditional optical microscopy observation techniques. However, in recent years, it has been possible to observe microstructures beyond the Rayleigh criterion by analysing the phase distribution of light. This study investigated the physical reasons why phase analysis makes this new analysis technique possible using simulations. The results confirmed that the phase component of the zero-order diffracted light reflected from the microstructure and able to pass through the lens system contained phase information related to the shape of the measured object. Analysis of this information demonstrates the possibility of realising super-resolution based on speckle interferometry. Full article
(This article belongs to the Special Issue Advanced Optical Microscopy and Imaging Technology)
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