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Pathogens
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One Health Perspectives on Foodborne Pathogens: Evolution, Resistance, and Prevention
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One Health Perspectives on Foodborne Pathogens: Evolution, Resistance, and Prevention

A special issue of Pathogens (ISSN 2076-0817). This special issue belongs to the section "Bacterial Pathogens".

Deadline for manuscript submissions: 31 March 2026 | Viewed by 2991

Special Issue Editor

Dr. Daniel F. M. Monte

E-Mail Website
Guest Editor
Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, CVM Research Building RM 472, 1051 William Moore Dr., Raleigh, NC 27607, USA
Interests: genomic surveillance; foodborne pathogens; AMR; virulence; One Health

Special Issue Information

Dear Colleagues,

The One Health approach addresses foodborne pathogens by integrating human, animal, and environmental health perspectives to enhance public health outcomes. This framework acknowledges that foodborne pathogens, such as Salmonella and Campylobacter, circulate across these interconnected domains, evolving through gene transfer and adapting to antimicrobial agents used in agriculture and medicine. Antimicrobial resistance (AMR) in these pathogens poses a significant threat, as resistant strains can spread between animals, humans, and environments, complicating treatment efforts and exacerbating health risks. By adopting a One Health perspective, researchers and policymakers aim to monitor and mitigate AMR and pathogen transmission through coordinated surveillance, safer agricultural practices, and targeted interventions. This comprehensive view highlights the need for cross-sector collaboration to address food safety, develop effective prevention strategies, and ensure sustainable approaches to antimicrobial use.

Dr. Daniel F. M. Monte
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Pathogens is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • genomic surveillance
  • foodborne pathogens
  • AMR
  • virulence
  • One Health

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Published Papers (4 papers)

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Research

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13 pages, 4200 KB  
Article
AI-Enhanced FT-IR Spectroscopy: Evaluation of a Novel Tool for High-Throughput Serovar Typing of Salmonella enterica subsp. enterica in Croatia
by Maja Dopuđ, Sandra Šuto, Dora Tomašković, Lucija Hlebić, Lovran Peinović, Silvio Špičić, Maja Zdelar Tuk, Irena Reil, Andrea Humski, Gordan Kompes, Silvija Šoprek Strugar, Bojan Papić, Jana Avberšek, Andrzej Mikolajczak and Sanja Duvnjak
Pathogens 2025, 14(9), 856; https://doi.org/10.3390/pathogens14090856 - 28 Aug 2025
Viewed by 548
Abstract
Rapid and accurate serotyping of Salmonella (S.) enterica subsp. enterica serovars is essential for effective public health surveillance, outbreak control, and food safety management. Traditional serotyping, although considered the gold standard, is time-consuming, technically demanding, and costly. This study aimed to [...] Read more.
Rapid and accurate serotyping of Salmonella (S.) enterica subsp. enterica serovars is essential for effective public health surveillance, outbreak control, and food safety management. Traditional serotyping, although considered the gold standard, is time-consuming, technically demanding, and costly. This study aimed to evaluate the applicability of artificial intelligence (AI)-enhanced Fourier-transform infrared (FT-IR) spectroscopy using an IR Biotyper (Bruker Daltonics, Bremen, Germany) for the rapid and accurate serotyping of Salmonella enterica subsp. enterica isolates in Croatia. Materials and Methods: A total of 143 isolates representing different S. enterica serovars of human and food origin were analysed using the IR Biotyper. Each strain was tested in three biological and at least three technical replicates. The obtained results were compared with traditional serotyping according to the Kauffmann–White–Le Minor scheme. Isolate identification at the genus level was further confirmed by MALDI-TOF mass spectrometry. Results: The IR Biotyper demonstrated high reproducibility and complete concordance with standard serotyping methods, enabling accurate differentiation of the most prevalent S. enterica serovars in Croatia. Conclusions: Our findings demonstrate the applicability of FT-IR in routine laboratory work, with the potential to reduce typing time, reduce the number of strains, and lower overall costs required for epidemiological surveillance within the One Health approach. Full article
(This article belongs to the Special Issue One Health Perspectives on Foodborne Pathogens: Evolution, Resistance, and Prevention)
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19 pages, 979 KB  
Article
Genetic Factors of Campylobacter jejuni Required for Its Interactions with Free-Living Amoeba
by Deepti Pranay Samarth, Asim Z. Abbasi and Young Min Kwon
Pathogens 2025, 14(6), 546; https://doi.org/10.3390/pathogens14060546 - 31 May 2025
Viewed by 589
Abstract
Acanthamoeba, a free-living amoeba ubiquitous in environmental water, has been considered as the environmental reservoir of certain bacterial pathogens, including Campylobacter jejuni, an intracellular human pathogen causing self-limiting gastroenteritis. Acanthamoeba-C. jejuni interaction mechanisms may help clarify how the otherwise [...] Read more.
Acanthamoeba, a free-living amoeba ubiquitous in environmental water, has been considered as the environmental reservoir of certain bacterial pathogens, including Campylobacter jejuni, an intracellular human pathogen causing self-limiting gastroenteritis. Acanthamoeba-C. jejuni interaction mechanisms may help clarify how the otherwise fastidious bacterium C. jejuni survives in environmental waters. In this study, we constructed single deletion mutants of C. jejuni strain 81–176 for the 10 selected genes (motAB, ciaB, kpsE, virB11, cheY, flaAB, cstII, docB, sodB, and cadF) previously shown to be important for the interaction (invasion and intracellular survival) of C. jejuni with mammalian hosts. We used a modified gentamicin protection assay to quantify the internalization and intracellular survival of these mutants and the wild type with the two species of Acanthamoeba (A. castellanii and A. polyphaga). Both internalization and intracellular survival were significantly lower for all mutants compared to the wild type with both amoeba strains, except for ΔcstII in the internalization assay with A. castellanii (p < 0.05). The results of this study highlight that the mechanisms used by C. jejuni to interact with mammalian hosts are conserved in its interactions with amoeba hosts. This understanding may be useful in developing effective strategies to reduce the transmission of C. jejuni to chickens through drinking water. Full article
(This article belongs to the Special Issue One Health Perspectives on Foodborne Pathogens: Evolution, Resistance, and Prevention)
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20 pages, 2323 KB  
Article
Temperature Influences Antimicrobial Resistance and Virulence of Vibrio parahaemolyticus Clinical Isolates from Quebec, Canada
by Feriel C. Mahieddine, Annabelle Mathieu-Denoncourt and Marylise Duperthuy
Pathogens 2025, 14(6), 521; https://doi.org/10.3390/pathogens14060521 - 23 May 2025
Cited by 1 | Viewed by 1248
Abstract
Climate change and ocean warming have a huge impact on microbial communities, leading to an increased prevalence of Vibrio parahaemolyticus infections in northern America. V. parahaemolyticus is an inhabitant of aquatic environments and is associated with fishes and shellfishes, particularly with oysters. It [...] Read more.
Climate change and ocean warming have a huge impact on microbial communities, leading to an increased prevalence of Vibrio parahaemolyticus infections in northern America. V. parahaemolyticus is an inhabitant of aquatic environments and is associated with fishes and shellfishes, particularly with oysters. It causes gastrointestinal infection through consumption of contaminated seafood, as well as wound infections or septicemia. Temperature is known to affect virulence and persistence factors in V. parahaemolyticus. In this study, twenty clinical strains isolated form sick patients in Quebec, Canada, were characterized for persistence and virulence factor production at different temperatures to assess the impact of a switch from sea water to the human body on them. Their capacity to produce biofilm, hemolysins, and membrane vesicles as well as their motility and antibiotic resistance at 20 °C and 37 °C were assessed. Our results revealed that while temperature had little effect on vesicle production, it significantly influenced their growth, antimicrobial resistance, biofilm formation, and motility. Additionally, all V. parahaemolyticus strains produced hemolysins at 37 °C but not under environmental conditions. Full article
(This article belongs to the Special Issue One Health Perspectives on Foodborne Pathogens: Evolution, Resistance, and Prevention)
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Review

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14 pages, 376 KB  
Review
Microbial Quality of Leafy Greens Grown Under Soilless Production Systems
by Robert Korir Cheruiyot and Abraham Fikru Mechesso
Pathogens 2025, 14(9), 943; https://doi.org/10.3390/pathogens14090943 - 18 Sep 2025
Viewed by 133
Abstract
This review examines the microbiological diversity and food safety implications of soilless production systems, particularly aquaponics and hydroponics, which are gaining popularity as efficient methods for producing fresh produce in controlled environments. Despite their advantages, a limited understanding of the microbiological quality and [...] Read more.
This review examines the microbiological diversity and food safety implications of soilless production systems, particularly aquaponics and hydroponics, which are gaining popularity as efficient methods for producing fresh produce in controlled environments. Despite their advantages, a limited understanding of the microbiological quality and potential food safety risks associated with leafy greens grown in these systems remains. By analyzing published studies, we summarize evidence of microbial contamination in aquaponic and hydroponic environments and their crops, noting that various factors may facilitate pathogen survival and spread to edible plant parts. The operational practices and environmental conditions can promote pathogen introduction through multiple routes, including contaminated fingerlings, fish feed, recirculating contaminated water, pest intrusion, improper handling, and poor worker hygiene. The studies reviewed detected pathogens that pose public health risks, including Salmonella spp., Listeria monocytogenes, Pseudomonas aeruginosa, and Shiga toxin-producing Escherichia coli O157:H7, as well as various molds. These potentially contaminated fresh produces are often consumed raw, presenting significant food safety and public health risks that demand further investigation and mitigation strategies to ensure consumer protection while maintaining the benefits of soilless agriculture. Full article
(This article belongs to the Special Issue One Health Perspectives on Foodborne Pathogens: Evolution, Resistance, and Prevention)
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