One Health Surveillance of Sand-Fly and Leishmania Transmission

A special issue of Pathogens (ISSN 2076-0817). This special issue belongs to the section "Parasitic Pathogens".

Deadline for manuscript submissions: 20 December 2026 | Viewed by 414

Editors


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Guest Editor
Centro de Ciências da Saúde e do Desporto, Universidade Federal do Acre, Rio Branco 69920-900, Brazil
Interests: sandflies; Leishmania; American cutaneous leishmaniasis

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Guest Editor
Instituto Evandro Chagas, Ananindeua 67130-000, Brazil
Interests: sandflies; Leishmania; American cutaneous leishmaniasis

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Guest Editor
Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina, Florianópolis 88040-900, Brazil
Interests: Leishmania; visceral Leishmaniasis; tegumentary leishmaniasis; diagnosis; sandflies

Special Issue Information

Dear Colleagues,

Phlebotomine sandflies are insects of public health importance because they transmit the causative agents of leishmaniasis, which connects humans, animals, and vectors in its transmission cycles. Given the wide variety of parasites and hosts involved in this complex of diseases, leishmaniasis is a key focus in the One Health approach, as its control and preventive measures must consider integrated actions aimed at human, animal, and environmental well-being.

This Special Issue will address One Health as a surveillance approach to sand flies and leishmaniasis and advance new insights on the epidemiological and environmental aspects of parasites and vectors. We invite you to submit original articles and review articles related to the above topics. 

Prof. Dr. Andreia Brilhante
Dr. Thiago Vasconcelos Dos Santos
Prof. Dr. Patrícia Flávia Quaresma
Guest Editors

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Keywords

  • phlebotomine sandflies
  • Leishmania
  • leishmaniasis
  • One Health
  • parasites and vectors

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Published Papers (1 paper)

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Research

13 pages, 664 KB  
Article
Baseline Characterization of the Gut Microbiota of Field and Colony Populations of Phlebotomus tobbi and Preliminary Assessment of the Anti-Leishmanial Activity of Cultivable Bacteria
by Mehmet Karakuş, Ayda Yılmaz, Mert Okbay, Metin Pekağırbaş and Ozge Erisoz Kasap
Pathogens 2026, 15(7), 658; https://doi.org/10.3390/pathogens15070658 (registering DOI) - 23 Jun 2026
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Abstract
Sand fly midgut microbiota plays a critical role in shaping Leishmania development and vector competence, yet functional evidence from natural vector populations remains limited. In this study, sand flies were collected between 2020 and 2022 in Cukurova region, Türkiye to characterize the gut [...] Read more.
Sand fly midgut microbiota plays a critical role in shaping Leishmania development and vector competence, yet functional evidence from natural vector populations remains limited. In this study, sand flies were collected between 2020 and 2022 in Cukurova region, Türkiye to characterize the gut bacterial composition of Phlebotomus tobbi and evaluate the anti-leishmanial potential of cultivable isolates. A total of 1739 sand flies were captured (878 females, 861 males), of which Ph. tobbi was the predominant species (n = 1312). 16S rRNA amplicon sequencing (V4–V6) showed that the gut microbiota was dominated by Proteobacteria, with Erwinia aphidicola/persicina representing the most abundant species across all analyzed groups. Fourteen cultivable bacterial species were identified by MALDI-TOF MS, including Serratia liquefaciens, Pantoea agglomerans, and Micrococcus luteus. Functional XTT assays against Leishmania infantum promastigotes demonstrated variable inhibitory activity among isolates. The strongest leishmanicidal effects were observed with S. liquefaciens (32.3%) and M. luteus (28.8%). Morphological examination confirmed promastigote rounding and cell death in isolates showing >25% activity. These findings define the gut bacterial landscape of Ph. tobbi in an endemic region and identify bacterial taxa with in vitro anti-leishmanial activity, highlighting their potential for future microbiota-based or paratransgenic control strategies. Full article
(This article belongs to the Special Issue One Health Surveillance of Sand-Fly and Leishmania Transmission)
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