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Human Protein Requirements and New Findings Regarding the Factors That Influence Them

A special issue of Nutrients (ISSN 2072-6643). This special issue belongs to the section "Proteins and Amino Acids".

Deadline for manuscript submissions: 20 January 2026 | Viewed by 945

Special Issue Editor


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Graduate School of Health and Sports Science, Juntendo University, Inzai 2701695, Chiba, Japan
Interests: human nutrition; gut microbiota; sports science; immunology; exercise physiology
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Special Issue Information

Dear Colleagues,

Various factors influence human nitrogen balance, including age, sex, climate, protein source, energy availability, and physical activity.

Many questions attract researchers:

  • The body can adapt to a consistent level of protein intake within up to two weeks. When the body adapts to a protein-free diet, some nitrogen loss is inevitable. In the nitrogen balance method, the amount of nitrogen required by the body is usually calculated based on the assumption that the miscellaneous loss of nitrogen from the skin and other sources is the same as the loss that occurs when the body adapts to a protein-free diet. However, when the body has fully adapted to a certain level of protein intake, is the miscellaneous loss the same as that occurring when the body is on a protein-free diet?
  • Do protein requirements change with age as resting metabolic rate decreases?
  • The texture of the diet influences the secretion of mucin, which is rich in threonine. Does the texture of the diet—whether regular, modified, or fluid—affect the body’s protein and amino acid requirements?
  • The gut microbiota can fix atmospheric nitrogen and/or produce essential amino acids. Does gut microbiota affect the requirement?
  • The latest recommended essential amino acid pattern was established by the WHO/FAO/UNU in 2007. Could new findings improve this scoring pattern?
  • Topics in sports nutrition include the protein requirements of physically active athletes and strategies to retain or add skeletal muscle during weight loss.

This Special Issue collects papers on human nitrogen/protein requirements from various perspectives. Novel findings related to this theme are also welcome.

Prof. Dr. Yoshio Suzuki
Guest Editor

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Keywords

  • nitrogen balance
  • nitrogen requirement
  • protein requirement
  • sex
  • climate
  • protein source
  • health
  • injured
  • physical activity
  • age
  • exercise
  • amino acid
  • weight loss
  • adaptation
  • energy availability

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Published Papers (1 paper)

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20 pages, 1144 KB  
Systematic Review
Nitrogen Requirements in Healthy Adults: A Systematic Review and Meta-Analysis of Nitrogen Balance Studies
by Daisuke Suzuki, Kohsuke Hayamizu, Chiharu Uno, Yoko Hasegawa, Masashi Kuwahata, Yasuhiro Kido and Yoshio Suzuki
Nutrients 2025, 17(16), 2615; https://doi.org/10.3390/nu17162615 - 12 Aug 2025
Viewed by 765
Abstract
Background: Nitrogen balance studies have traditionally been used to estimate protein requirements in adults. However, ethical and practical constraints have made new studies increasingly difficult to conduct. This systematic review and meta-analysis aimed to compile and analyze the most comprehensive individual-level dataset to [...] Read more.
Background: Nitrogen balance studies have traditionally been used to estimate protein requirements in adults. However, ethical and practical constraints have made new studies increasingly difficult to conduct. This systematic review and meta-analysis aimed to compile and analyze the most comprehensive individual-level dataset to date. Methods: We included 31 studies that reported nitrogen intake and excretion data for healthy adults. Studies were selected based on strict eligibility criteria that required data from at least three intake levels per individual. Nitrogen requirements were estimated using regression analysis. In total, data from 395 individuals were analyzed. We used a random effects model for the meta-analysis. Subgroup comparisons and meta-regression were conducted based on sex, age, climate, and protein source. Results: The overall mean nitrogen requirement was 104.2 mg N/kg/day. No significant differences were found by sex, age group (<60 vs. ≥60 years), climate (temperate vs. tropical), or protein source (animal, plant, or mixed). Subgroup and meta-regression analyses did not reveal consistent moderator effects. Substantial heterogeneity was observed (I2 > 90%). Conclusions: This analysis provides the most extensive compilation of individual-level nitrogen balance data to date. While the average nitrogen requirement was consistent with previous estimates, the high heterogeneity limits definitive conclusions. Nonetheless, this dataset provides a valuable foundation for revising protein intake recommendations and guiding future research on human nitrogen metabolism in the absence of new studies. Full article
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