Special Issue "The Genetic Changes Induced by Engineered Manufactured Nanomaterials (EMNs)"
Deadline for manuscript submissions: 31 August 2021.
Interests: biotechnology; nanotechnology; bio-nanotechnology; phytoremediation; plant stress; heavy metals and metalloids; biochar
Special Issues and Collections in MDPI journals
The possibility that engineered manufactured nanomaterials (ENMs) can be harmful to the genetic materials of living individuals has been raised by several experiments, but it is, however, still controversial. In fact, there is also evidence that nanoparticles are not genotoxic and do not interfere with the genetic materials of organisms. It is of extreme importance to establish which nanomaterials have the potential to exert harmful effects on DNA in any type of living organisms, from simple prokaryotes to complex eukaryotes, starting from model organisms.
The aims and scopes of this Special Issue are to (1) highlight the research applications that find out which ENMs are genotoxic and which are the more susceptible organisms or cell lines, and (2) to pinpoint reliable methods to establish the genotoxicity of ENMs.
Dr. Marta Marmiroli
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Nanomaterials is an international peer-reviewed open access monthly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.
- Engineered manufactured nanomaterials (ENMs)
- DNA fingerprinting
- Model organisms
- Genomic interaction
The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.
Title: Comparative analysis of proteins regulated during cadmium sulphide quantum dots response in Arabidopsis thaliana wild type and tolerant mutants
Authors: Gallo Valentina, Villani Marco, Zappettini Andrea, Marmiroli Nelson, Marmiroli Marta Valentina Gallo1, Andrea Zappettini2, Marco Villani2, Nelson Marmiroli 1,3, Marta Marmiroli 1 *
Affiliation: 1 Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, 4 Parma, Italy 2 Institute of Materials for Electronics and Magnetism (IMEM), National Research Council (CNR), 6 Parma, Italy 3 The Italian National Interuniversity Consortium for Environmental Sciences (CINSA), Parma, Italy
Abstract: Understanding the interaction between plants and engineered nanomaterials (ENM) is of relevance as among the latter are large applications in agriculture whereas others are more suitable for industry and risk to become environmental pollutants. In a previous work, two independent A. thaliana Ac/Ds transposons insertional mutants, atnp01 and atnp02, displaying a greater level of tolerance than the wild type plant to cadmium sulphide quantum dots (CdS QDs) were isolated and the tolerance response was characterized at physiological, genetic and transcriptomic levels. In this work, a comparative analysis was performed on crude protein extracts, obtained from plantlets of the two mutants and of wild type, grown on agarized MS, treated with 80 mg/L-1 CdS QDs. A comparative protein analysis was performed by 2D- PAGE and proteins more relevant were characterized by MALDI-TOF/TOF mass spectrometry. The results obtained showed that the two mutants had a different response to treatments in terms of quantity and quality of up and down regulated proteins. This difference becomes more striking when considering the wild type for comparison. A network analysis of the differentially regulated proteins involved some of those encoded by putative genes affected by transposition in atnp01 and atnp02 and showed that the genes visited by the transposons are responsible for the regulation of some proteins found in this study like NFU3 (Nifu-like protein 3), involved in chloroplast assembly, ELO3 (Elongator Complex 3), involved in transcriptional elongation, CHLI2 (magnesium-chelate subunit2) involved in chlorophyll biosynthesis, and PP2C (Protein phosphatase 2C) that mediates abiotic stress response.
Title: A multi-target approach to investigate the potential of “genotoxicity-free” nanoparticles for environmental remediation
Authors: Margherita Bernardeschi1★, Patrizia Guidi1★, Mara Palumbo1, Massimo Genovese2, Michela Alfè3, Valentina Gargiulo3, Paolo Lucchesi1, Vittoria Scarcelli1, Elisa Bergami4, Barbara Bonelli5, Ilaria Corsi4, Giada Frenzilli1*
Affiliation: 1 Department of Clinical and Experimental Medicine, Section of Applied Biology and Genetics. University of Pisa, Pisa, Italy; [email protected] (P.G); [email protected] (M.B.); [email protected] (M.P.); [email protected] (V.S.); [email protected] (P.L.); [email protected] (G.F.) 2 Department of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, Florence, Italy; [email protected] 3 Institute of Science and Technology for Sustainable Energy and Mobility STEMS-CNR. Naples, Italy, [email protected], [email protected] 4 Department of Applied Science and Technology, Politecnic of Turin, Italy, [email protected] 5 Department of Physical, Earth and Environmental Sciences and INSTM Local Unit, University of Siena, Siena, Italy; [email protected] ★Both authors have equally contributed to this manuscript
Abstract: Benzo(a)pyrene (B(a)P) is a well-known genotoxic agent, whose removal from environmental matrices is mandatory, as well as the setting up of a cleaning strategy harmless for human and environmental health. The potential application of nanoparticles (NPs) in the remediation of polluted environments is of increasing interest. Genotoxicity of titanium dioxide (n-TiO2) in two crystalline forms (pure anatase and a mixture of anatase and rutile) and carbon black-derived hydrophilic NPs (HNP) was investigated; their potential use in reducing organic contaminant-induced genotoxicity was also assessed. Mussel (Mytilus galloprovincialis) gill biopsies were in vitro exposed to B(a)P (2 μg/ml) alone and in combination with the selected NPs (50 µg/ml n-TiO2, 10 µg/ml HNP). Primary DNA damage was evaluated through Comet assay. Micronucleated cells and morphological nuclear abnormalities were assessed using Micronucleus-Cytome assay. Cellular uptake was investigated by Transmission Electron Microscopy. Pure anatase n-TiO2 resulted as the most suitable for remediation, being able to reduce B(a)P-induced DNA damage without side-effects.